Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,489,210	T→C	K324R (AAG→AGG)	argD ←	N‑acetylornithine aminotransferase/N‑succinyldiaminopimelate aminotransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,489,210	0	T	C	100.0%	265.0 / NA	71	K324R (AAG→AGG)	argD	N‑acetylornithine aminotransferase/N‑succinyldiaminopimelate aminotransferase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (33/38);  total (33/38)

CACCATTACGCCAGCCTCTGCGCCCGCATACAGGAAATCACGCGCCCGACCTTTGTACTGTGGTTTCAGCTCTGCGCCAATCAACAGCCCCATACCGCGAATATCGCTAAATACATCGTACTGCTGATCGATCTTCTGCAGATGGTC‑AACAAAACGCTGGCGTTTCGCCTGAATGCCTTCCAGCACTTCAGGGGTATTGATGATATCAAACGCCGCCCCCGCTACTGCACAGGCCAGAGGATTACCGCCGTAGGTGGAACCGTGAGAACC  >  NC_000913/3489077‑3489346                                                                                                                                      |                                                                                                                                          cACCATTACGCCAGCCTCTGCGCCCGCATACAGGAAATCACGCGCCCGACCTTTGTACTGTGGTTTCAGCTCTGCGCCAATCAACAGCCCCATACCGCGAATATCGCTAAATACATCGTACTGCTGATCGATCCTCTGCa                                                                                                                                     <  2:958757/140‑1 (MQ=255)      ttACGCCAGCCTCTGCGCCCGCATACAGGAAATCACGCGCCCGACCTTTGTACTGTGGTTTCAGCTCTGCGCCAATCAACAGCCCCATACCGCGAATATCGCTAAATACATCGTACTGCTGATCGATCCTCTGCAGATgg                                                                                                                                >  1:548022/1‑140 (MQ=255)      ttACGCCAGCCTCTGCGCCCGCATACAGGAAATCACGCGCCCGACCTTTGTACTGTGGTTTCAGCTCTGCGCCAATCAACAGCCCCATACCGCGAATATCGCTAAATACATCGTACTGCTGATCGATCCTCTGCAGATgg                                                                                                                                >  2:650782/1‑140 (MQ=255)       tACGCCAGCCTCTGCGCCCGCATACAGGAAATCACGCGCCCGACCTTTGTACTGTGGTTTCAGCTCTGCGCCAATCAACAGCCCCATACCGCGAATATCGCTAAATACATCGTACTGCTGATCGATCCTCTGCAGATGGt                                                                                                                               <  1:1081234/140‑1 (MQ=255)         cGCCAGCCTCTGCGCCCGCATACAGGAAATCACGCGCCCGACCTTTGTACTGTGGTTTCAGCTCTGCGCCAATCAACAGCCCCATACCGCGAATATCGCTAAATACATCGTACTGCTGATCGATCCTCTGCAGATGGTc‑a                                                                                                                            >  2:508199/1‑140 (MQ=255)              gCCTCTGCGCCCGCATACAGGAAATCACGCGCCCGACCTTTGTACTGTGGTTTCAGCTCTGCGCCAATCAACAGCCCCATACCGCGAATATCGCTAAATACATCGTACTGCTGATCGATCCTCTGCAGATGGTc‑aaca                                                                                                                         >  1:590545/1‑138 (MQ=255)              gCCTCTGCGCCCGCATACAGGAAATCACGCGCCCGACCTTTGTACTGTGGTTTCAGCTCTGCGCCAATCAACAGCCCCATACCGCGAATATCGCTAAATACATCGTACTGCTGATCGATCCTCTGCAGATGGTc‑aaca                                                                                                                         <  2:590545/138‑1 (MQ=255)              gCCTCTGCGCCCGCATACAGGAAATCACGCGCCCGACCTTTGTACTGTGGTTTCAGCTCTGCGCCAATCAACAGCCCCATACCGCGAATATCGCTAAATACATCGTACTGCTGATCGATCCTCTGCAGATGGTC‑AACaaa                                                                                                                       >  2:892552/1‑140 (MQ=255)              gCCTCTGCGCCCGCATACAGGAAATCACGCGCCCGACCTTTGTACTGTGGTTTCAGCTCTGCGCCAATCAACAGCCCCATACCGCGAATATCGCTAAATACATCGTACTGCTGATCGATCCTCTGCAGATGGTC‑AACaaa                                                                                                                       >  1:139577/1‑140 (MQ=255)              gCCTCTGCGCCCGCATACAGGAAATCACGCGCCCGACCTTTGTACTGTGGTTTCAGCTCTGCGCCAATCAACAGCCCCATACCGCGAATATCGCTAAATACATCGTACTGCTGATCGATCCTCTGCAGATGGTC‑AACaaa                                                                                                                       >  2:842373/1‑140 (MQ=255)              gCCTCTGCGCCCGCATACAGGAAATCACGCACCCGACCTTTGTACTGTGGTTTCAGCTCTGCGCCAATCAACAGCCCCATACCGCGAATATCGCTAAATACATCGTACTGCTGATCGATCCTCTGCAGATGGTC‑AACaaa                                                                                                                       >  1:34797/1‑140 (MQ=255)                          gCATACAGGAAATCACGCGCCCGACCTTTGTACTGTGGTTTCAGCTCTGCGCCAATCAACAGCCCCATACCGCGAATATCGCTAAATACATCGTACTGCTGATCGATCCTCTGCAGATGGTC‑AACAAAACGCTGGCGttt                                                                                                           >  2:179785/1‑140 (MQ=255)                           cATACAGGAAATCACGCGCCCGACCTTTGTACTGTGGTTTCAGCTCTGCGCCAATCAACAGCCCCATACCGCGAATATCGCTAAATACATCGTACTGCTGATCGATCCTCTGCAGATGGTC‑AACAAAACGCTGGCGTTTc                                                                                                          >  2:990578/1‑140 (MQ=255)                           cATACAGGAAATCACGCGCCCGACCTTTGTACTGTGGTTTCAGCTCTGCGCCAATCAACAGCCCCATACCGCGAATATCGCTAAATACATCGTACTGCTGATCGATCCTCTGCAGATGGTC‑AACAAAACGCTGGCGTTTc                                                                                                          >  2:1405241/1‑140 (MQ=255)                                  gAAATCACGCGCCCGACCTTTGTACTGTGGTTTCAGCTCTGCGCCAATCAACAGCCCCATACCGCGAATATCGCTAAATACATCGTACTGCTGATCGATCCTCTGCAGATGGTC‑AACAAAACGCTGGCGTTTCGCCTGaa                                                                                                   <  1:179785/140‑1 (MQ=255)                                  gAAATCACGCGCCCGACCTTTGTACTGTGGTTTCAGCTCTGCGCCAATCAACAGCCCCATACCGCGAATATCGCTAAATACATCGTACTGCTGATCGATCCTCTGCAGATGGTC‑AACAAAACGCTGGCGTTTCGCCTGaa                                                                                                   <  1:1152514/140‑1 (MQ=255)                                      tCACGCGCCCGACCTTTGTACTGTGGTTTCAGCTCTGCGCCAATCAACAGCCCCATACCGCGAATATCGCTAAATACATCGTACTGCTGATCGATCCTCTGCAGATGGTC‑AACAAAACGCTGGCGTTTCGCCTGAATg                                                                                                 >  2:596907/1‑138 (MQ=255)                                      tCACGCGCCCGACCTTTGTACTGTGGTTTCAGCTCTGCGCCAATCAACAGCCCCATACCGCGAATATCGCTAAATACATCGTACTGCTGATCGATCCTCTGCAGATGGTC‑AACAAAACGCTGGCGTTTCGCCTGAATg                                                                                                 <  1:596907/138‑1 (MQ=255)                                       cACGCGCCCGACCTTTGTACTGTGGTTTCAGCTCTGCGCCAATCAACAGCCCCATACCGCGAATATCGCTAAATACATCGTACTGCTGATTGATCCTCTGCAGATGGTC‑AACAAAACGCTGGCGTTTCGCCTGAATGCCt                                                                                              <  2:821180/140‑1 (MQ=255)                                         cgcgCCCGACCTTTGTACTGTGGTTTCAGCTCTGCGCCAATCAACAGCCCCATACCGCGAATATCGCTAAATACATCGTACTGCTGATCGATCCTCTGCAGATGGTC‑AACAAAACGCTGGCGTTTCGCCTGAATGCCTTc                                                                                            <  2:1384337/140‑1 (MQ=255)                                          gcgcCCGACCTTTGTACTGTGGTTTCAGCTCTGCGCCAATCAACAGCCCCATACCGCGAATATCGCTAAATACATCGTACTGCTGATCGATCCTCTGCAGATGGTC‑AACAAAACGCTGGCGTTTCGCCTGAATGCCTTcc                                                                                           >  1:1272959/1‑140 (MQ=255)                                            gcCCGACCTTTGTACTGTGGTTTCAGCTCTGCGCCAATCAACAGCCCCATACCGCGAATATCGCTAAATACATCGTACTGCTGATCGATCCTCTGCAGATGGTC‑AACAAAACGCTGGCGTTTCGCCTGAATGCCTTCCAg                                                                                         <  1:508199/140‑1 (MQ=255)                                            gcCCGACCTTTGTACTGTGGTTTCAGCTCTGCGCCAATCAACAGCCCCATACCGCGAATATCGCTAAATACATCGTACTGCTGATCGATCCTCTGCAGATGGTC‑AACAAAACGCTGGCGTTTCGCCTGAATGCCTTCCAg                                                                                         <  1:840959/140‑1 (MQ=255)                                            gcCCGACCTTTGTACTGTGGTTTCAGCTCTGCGCCAATCAACAGCCCCATACCGCGAATATCGCTAAATACATCGTACTGCTGATCGATCCTCTGCAGATGGTC‑AACAAAACGCTGGCGTTTCGCCTGAATGCCTTCCAg                                                                                         <  2:969500/140‑1 (MQ=255)                                                  ccTTTGTACTGTGGTTTCAGCTCTGCGCCAATCAACAGCCCCATACCGCGAATATCGCTAAATACATCGTACTGCTGATCGATCCTCTGCAGATGGTC‑AACAAAACGCTGGCGTTTCGCCTGAATGCCTTCCAGCACTTc                                                                                   <  1:537620/140‑1 (MQ=255)                                                    tttGTACTGTGGTTTCAGCTCTGCGCCAATCAACAGCCCCATACCGCGAATATCGCTAAATACATCGTACTGCTGATCGATCCTCTGCAGATGGTC‑AACAAAACGCTGGCGTTTCGCCTGAATGCCTTCCAGCACTTCAg                                                                                 <  1:463222/140‑1 (MQ=255)                                                      tGTACTGTGGTTTCAGCTCTGCGCCAATCAACAGCCCCATACCGCGAATATCGCTAAATACATCGTACTGCTGATCGATCCTCTGCAGATGGTC‑AACAAAACGCTGGCGTTTCGCCTGAATGCCTTCCAGCACTTCAggg                                                                               <  1:650782/140‑1 (MQ=255)                                                        tACTGTGGTTTCAGCTCTGCGCCAATCAACAGCCCCATACCGCGAATATCGCTAAATACATCGTACTGCTGATCGATCCTCTGCAGATGGTC‑AACAAAACGCTGGCGTTTCGCCTGAATGCCTTCCAGCACTTCAggg                                                                               >  2:759542/1‑138 (MQ=255)                                                        tACTGTGGTTTCAGCTCTGCGCCAATCAACAGCCCCATACCGCGAATATCGCTAAATACATCGTACTGCTGATCGATCCTCTGCAGATGGTC‑AACAAAACGCTGGCGTTTCGCCTGAATGCCTTCCAGCACTTCAggg                                                                               <  1:759542/138‑1 (MQ=255)                                                             tgGTTTCAGCTCTGCGCCAATCAACAGCCCCATACCGCGAATATCGCTAAATACATCGTACTGCTGATCGATCCTCTGCAGATGGTC‑AACAAAACGCTGGCGTTTCGCCTGAATGCCTTCCAGCACTTCAGGGGTa                                                                            >  2:1077830/1‑136 (MQ=255)                                                             tgGTTTCAGCTCTGCGCCAATCAACAGCCCCATACCGCGAATATCGCTAAATACATCGTACTGCTGATCGATCCTCTGCAGATGGTC‑AACAAAACGCTGGCGTTTCGCCTGAATGCCTTCCAGCACTTCAGGGGTa                                                                            <  1:1077830/136‑1 (MQ=255)                                                             tgGTTTCAGCTCTGCGCCAATCAACAGCCCCATACCGCGAATATCGCTAAATACATCGTACTGCTGATCGATCCTCTGCAGATGGTC‑AACAAAACGCTGGCGTTTCGCCTGAATGCCTTCCAGCACTTCAGGGGTATtga                                                                        <  1:892552/140‑1 (MQ=255)                                                             tgGTTTCAGCTCTGCGCCAATCAACAGCCCCATACCGCGAATATCGCTAAATACATCGTACTGCTGATCGATCCTCTGCAGATGGTC‑AACAAAACGCTGGCGTTTCGCCTGAATGCCTTCCAGCACTTCAGGGGTATtga                                                                        >  1:1329644/1‑140 (MQ=255)                                                                tttCAGCTCTGCGCCAATCAACAGCCCCATACCGCGAATATCGCTAAATACATCGTACTGCTGATCGATCCTCTGCAGATGGTC‑AACAAAACGCTGGCGTTTCGCCTGAATGCCTTCCAGCACTTCAg                                                                                 <  1:840507/128‑1 (MQ=255)                                                                tttCAGCTCTGCGCCAATCAACAGCCCCATACCGCGAATATCGCTAAATACATCGTACTGCTGATCGATCCTCTGCAGATGGTC‑AACAAAACGCTGGCGTTTCGCCTGAATGCCTTCCAGCACTTCAg                                                                                 >  2:840507/1‑128 (MQ=255)                                                                tttCAGCTCTGCGCCAATCAACAGCCCCATACCGCGAATATCGCTAAATACATCGTACTGCTGATCGATCCTCTGCAGATGGTC‑AACAAAACGCTGGCGTTTCGCCTGAATGCCTTCCAGCACTTCAGGGGTATtgatga                                                                     >  2:1398189/1‑140 (MQ=255)                                                                    aGCTCTGCGCCAATCAACAGCCCCATACCGCGAATATCGCTAAATACATCGTACTGCTGATCGATCCTCTGCAGATGGTC‑AACAAAACGCTGGCGTTTCGCCTGAATGCCTTCCAGCACTTCAGGGGTATTGATGATATc                                                                 >  1:590560/1‑140 (MQ=255)                                                                       tctGCGCCAATCAACAGCCCCATACCGCGAATATCGCTAAATACATCGTACTGCTGATCGATCCTCTGCAGATGGTC‑AACAAAACGCTGGCGTTTCGCCTGAATGCCTTCCAGCACTTCAGGGGTATTGATGATATCaaa                                                              >  2:569825/1‑140 (MQ=255)                                                                             ccAATCAACAGCCCCATACCGCGAATATCGCTAAATACATCGTACTGCTGATCGATCCTCTGCAGATGGTC‑AACAAAACGCTGGCGTTTCGCCTGAATGCCTTCCAGCACTTCAGGGGTATTGATGATATCAAAcgccgc                                                        <  1:842373/140‑1 (MQ=255)                                                                             ccAATCAACAGCCCCATACCGCGAATATCGCTAAATACATCGTACTGCTGATCGATCCTCTGCAGATGGTC‑AACAAAACGCTGGCGTTTCGCCTGAATGCCTTCCAGCACTTCAGGGGTATTGATGATATCAAAcgccgc                                                        <  2:548022/140‑1 (MQ=255)                                                                                aTCAACAGCCCCATACCGCGAATATCGCTAAATACATCGTACTGCTGATCGATCCTCTGCAGATGGTC‑AACAAAACGCTGGCGTTTCGCCTGAATGCCTTCCAGCACTTCAGGGGTATTGATGATATCAAACGCCGcccc                                                     <  2:34797/140‑1 (MQ=255)                                                                                aTCAACAGCCCCATACCGCGAATATCGCTAAATACATCGTACTGCTGATCGATCCTCTGCAGATGGTC‑AACAAAACGCTGGCGTTTCGCCTGAATGCCTTCCAGCACTTCAGGGGTATTGATGATATCAAACGCCGcccc                                                     <  1:1389523/140‑1 (MQ=255)                                                                                aTCAACAGCCCCATACCGCGAATATCGCTAAATACATCGTACTGCTGATCGATCCTCTGCAGATGGTC‑AACAAAACGCTGGCGTTTCGCCTGAATGCCTTCCAGCACTTCAGGGGTATTGATGATATCAAACGCCGcccc                                                     <  1:1398189/140‑1 (MQ=255)                                                                                aTCAACAGCCCCATACCGCGAATATCGCTAAATACATCGTACTGCTGATCGATCCTCTGCAGATGGTC‑AACAAAACGCTGGCGTTTCGCCTGAATGCCTTCCAGCACTTCAGGGGTATTGATGATATCAAACGCCGcccc                                                     <  1:255224/140‑1 (MQ=255)                                                                                aTCAACAGCCCCATACCGCGAATATCGCTAAATACATCGTACTGCTGATCGATCCTCTGCAGATGGTC‑AACAAAACGCTGGCGTTTCGCCTGAATGCCTTCCAGCACTTCAGGGGTATTGATGATATCAAACGCCGcccc                                                     <  2:437857/140‑1 (MQ=255)                                                                                aTCAACAGCCCCATACCGCGAATATCGCTAAATACATCGTACTGCTGATCGATCCTCTGCAGATGGTC‑AACAAAACGCTGGCGTTTCGCCTGAATGCCTTCCAGCACTTCAGGGGTATTGATGATATCAAACGCCGcccc                                                     >  2:255224/1‑140 (MQ=255)                                                                                aTCAACAGCCCCATACCGCGAATATCGCTAAATACATCGTACTGCTGATCGATCCTCTGCAGATGGTC‑AACAAAACGCTGGCGTTTCGCCTGAATGCCTTCCAGCACTTCAGGGGTATTGATGATATCAAACGCCGcccc                                                     <  2:1272959/140‑1 (MQ=255)                                                                                aTCAACAGCCCCATACCGCGAATATCGCTAAATACATCGTACTGCTGATCGATCCTCTGCAGATGGTC‑AACAAAACGCTGGCGTTTCGCCTGAATGCCTTCCAGCACTTCAGGGGTATTGATGATATCAAACGCCGcccc                                                     <  1:661166/140‑1 (MQ=255)                                                                                aTCAACAGCCCCATACCGCGAATATCGCTAAATACATCGTACTGCTGATCGATCCTCTGCAGATGGTC‑AACAAAACGCTGGCGTTTCGCCTGAATGCCTTCCAGCACTTCAGGGGTATTGATGATATCAAACGCCGcccc                                                     <  1:990578/140‑1 (MQ=255)                                                                                     cAGCCCCATACCGCGAATATCGCTAAATACATCGTACTGCTGATCGATCCTCTGCAGATGGTC‑AACAAAACGCTGGCGTTTCGCCTGAATGCCTTCCAGCACTTCGGGGGTATTGATGATATCAAACGCCGCCCCCGCTa                                                <  1:648284/140‑1 (MQ=255)                                                                                        ccccATACCGCGAATATCGCTAAATACATCGTACTGCTGATCGATCCTCTGCAGATGGTC‑AACAAAACGCTGGCGTTTCGCCTGAATGCCTTCCAGCACTTCAGGGGTATTGATGATATCAAACGCCGCCCCCGCTACTg                                             >  2:992969/1‑140 (MQ=255)                                                                                          ccATACCGCGAATATCGCTAAATACATCGTACTGCTGATCGATCCTCTGCAGATGGTC‑AACAAAACGCTGGCGTTTCGCCTGAATGCCTTCCAGCACTTCAGGGGTATTGATGATATCAAACGCCGCCCCCGCTACTGca                                           <  1:55827/140‑1 (MQ=255)                                                                                          ccATACCGCGAATATCGCTAAATACATCGTACTGCTGATCGATCCTCTGCAGATGGTC‑AACAAAACGCTGGCGTTTCGCCTGAATGCCTTCCAGCACTTCAGGGGTATTGATGATATCAAACGCCGCCCCCGCTACTGca                                           <  2:590560/140‑1 (MQ=255)                                                                                           cATACCGCGAATATCGCTAAATACATCGTACTGCTGATCGATCCTCTGCAGATGGTC‑AACAAAACGCTGGCGTTTCGCCTGAATGCCTTCCAGCACTTCAGGGGTATTGATGATATCAAACGCCGcccc                                                     >  1:552281/1‑129 (MQ=255)                                                                                           cATACCGCGAATATCGCTAAATACATCGTACTGCTGATCGATCCTCTGCAGATGGTC‑AACAAAACGCTGGCGTTTCGCCTGAATGCCTTCCAGCACTTCAGGGGTATTGATGATATCAAACGCCGcccc                                                     <  2:552281/129‑1 (MQ=255)                                                                                           cATACCGCGAATATCGCTAAATACATCGTACTGCTGATCGATCCTCTGCAGATGGTC‑AACAAAACGCTGGCGTTTCGCCTGAATGCCTTCCAGCACTTCAGGGGTATTGATGATATCAAACGCCGCCCCCGCTACTGcac                                          <  1:1216060/140‑1 (MQ=255)                                                                                             tACCGCGAATATCGCTAAATACATCGTACTGCTGATCGATCCTCTGCAGATGGTC‑AACAAAACGCTGGCGTTTCGCCTGAATGCCTTCCAGCACTTCAGGGGTATTGATGATATCAAACGCCGCCCCCGCTACTGca                                           <  1:5150/137‑1 (MQ=255)                                                                                             tACCGCGAATATCGCTAAATACATCGTACTGCTGATCGATCCTCTGCAGATGGTC‑AACAAAACGCTGGCGTTTCGCCTGAATGCCTTCCAGCACTTCAGGGGTATTGATGATATCAAACGCCGCCCCCGCTACTGca                                           >  2:5150/1‑137 (MQ=255)                                                                                               ccGCGAATATCGCTAAATACATCGTACTGCTGATCGATCCTCTGCAGATGGTC‑AACAAAACGCTGGCGTTTCGCCTGAATGCCTTCCAGCACTTCAGGGGTATTGATGATATCAAACGCCGCCCCCGCTACTGCACAGGc                                      <  2:1020834/140‑1 (MQ=255)                                                                                                  cgAATATCGCTAAATACATCGTACTGCTGATCGATCCTCTGCAGATGGTC‑AACAAAACGCTGGCGTTTCGCCTGAATGCCTTCCAGCACTTCAGGGGTATTGATGATATCAAACGCCGCCCCCGCTACTGCACAGGCCag                                   >  1:20848/1‑140 (MQ=255)                                                                                                  cgAATATCGCTAAATACATCGTACTGCTGATCGATCCTCTGCAGATGGTC‑AACAAAACGCTGGCGTTTCGCCTGAATGCCTTCCAGCACTTCAGGGGTATTGATGATATCAAACGCCGCCCCCGCTACTGCACAGGCCag                                   >  2:1014395/1‑140 (MQ=255)                                                                                                  cgAATATCGCTAAATACATCGTACTGCTGATCGATCCTCTGCAGATGGTC‑AACAAAACGCTGGCGTTTCGCCTGAATGCCTTCCAGCACTTCAGGGGTATTGATGATATCAAACGCCGCCCCCGCTACTGCACAGGCCag                                   >  2:174504/1‑140 (MQ=255)                                                                                                         cGCTAAATACATCGTACTGCTGATCGATCCTCTGCAGATGGTC‑AACAAAACGCTGGCGTTTCGCCTGAATGCCTTCCAGCACTTCAGGGGTATTGATGATATCAAACGCCGCCCCCGCTACTGCACAGGCCAGAGGATTa                            <  1:569825/140‑1 (MQ=255)                                                                                                            tAAATACATCGTCCTGCTGATCGATCCTTCGCA‑AGCGTCAAACAAAACGCTGGCGTTTCGCCTGAATGCCTTCCTTTACTACAGGGGTATTGATGATATAAAAGGCCGCCCCCGCTACGGCAGAGCGCAGAGGTTTAccg                         <  2:1223437/140‑1 (MQ=255)                                                                                                              aaTACATCGTACTGCTGATCGATCCTCTGCAGATGGTC‑AACAAAACGCTGGCGTTTCGCCTGAATGCCTTCCAGCACTTCAGGGGTATTGATGATATCAAACGCCGCCCCCGCTACTGCACAGGCCAGAGGATTAccgcc                       >  2:265963/1‑140 (MQ=255)                                                                                                                     cGTACTGCTGATCGATCCTCTGCAGATGGTC‑AACAAAACGCTGGCGTTTCGCCTGAATGCCTTCCAGCACTTCAGGGGTATTGATGATATCAAACGCCGCCCCCGCTACTGCACAGGCCAGAGGATTACCGCCGTAGGTg                >  2:1183046/1‑140 (MQ=255)                                                                                                                       tACTGCTGATCGATCCTCTGCAGATGGTC‑AACAAAACGCTGGCGTTCCGCCTGAATGCCTTCCAGCACTTCAGGGTTATGGATGATATCAACCGCCGCCCCCGCTCCGGCACAGGCCAGAGGATTACCGCCGTAGGTGGa              <  1:610820/140‑1 (MQ=255)                                                                                                                        aCTGCTGATCGATCCTCTGCAGATGGTC‑AACAAAACGCTGGCGTTTCGCCTGAATGCCTTCCAGCACTTCAGGGGTATTGATGATATCAAACGCCGCCCCCGCTACTGCACAGGCCAGAGGATTACCGCCGTAGGTGGaa             <  1:180948/140‑1 (MQ=255)                                                                                                                            ctgATCGATCCTCTGCAGATGGTC‑AACAAAACGCTGGCGTTTCGCCTGAATGCCTTCCAGCACTTCAGGGGTATTGATGATATCAAACGCCGCCCCCGCTACTGCACAGGCCAGAGGATTACCGCCGTAGGTGGAACCGt         >  2:610820/1‑140 (MQ=255)                                                                                                                            ctgATCGATCCTCTGCAGATGGTC‑AACAAAACGCTGGCGTTTCGCCTGAATGCCTTCCAGCACTTCAGGGGTATTGATGATATCAAACGCCGCCCCCGCTACTGCACAGGCCAGAGGATTACCGCCGTAGGTGGAACCGt         >  1:204300/1‑140 (MQ=255)                                                                                                                               atcgatcCTCTGCAGATGGTC‑AACAAAACGCTGGCGTTTCGCCTGAATGCCTTCCAGCACTTCAGGGGTATTGATGATATCAAACGCCGCCCCCGCTACTGCACAGGCCAGAGGATTAccgcc                       >  2:1273785/1‑123 (MQ=255)                                                                                                                               atcgatcCTCTGCAGATGGTC‑AACAAAACGCTGGCGTTTCGCCTGAATGCCTTCCAGCACTTCAGGGGTATTGATGATATCAAACGCCGCCCCCGCTACTGCACAGGCCAGAGGATTAccgcc                       <  1:1273785/123‑1 (MQ=255)                                                                                                                                   atcCTCTGCAGATGGTC‑AACAAAACGCTGGCGTTTCGCCTGAATGCCTTCCAGCACTTCAGGGGTATTGATGATATCAAACGCCGCCCCCGCTACTGCACAGGCCAGAGGATTACCGCCGTAGGTGGAACCGTGAGAAcc  >  1:679547/1‑140 (MQ=255)                                                                                                                                      |                                                                                                                                          CACCATTACGCCAGCCTCTGCGCCCGCATACAGGAAATCACGCGCCCGACCTTTGTACTGTGGTTTCAGCTCTGCGCCAATCAACAGCCCCATACCGCGAATATCGCTAAATACATCGTACTGCTGATCGATCTTCTGCAGATGGTC‑AACAAAACGCTGGCGTTTCGCCTGAATGCCTTCCAGCACTTCAGGGGTATTGATGATATCAAACGCCGCCCCCGCTACTGCACAGGCCAGAGGATTACCGCCGTAGGTGGAACCGTGAGAACC  >  NC_000913/3489077‑3489346

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑