Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,758,494	C→T	A456A (GCG→GCA)	selB ←	selenocysteyl‑tRNA‑specific translation elongation factor

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,758,494	0	C	T	100.0%	298.7 / NA	84	A456A (GCG→GCA)	selB	selenocysteyl‑tRNA‑specific translation elongation factor
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (40/44);  total (40/44)

AACAGTGGCTCTGCTTTTTGCCAGATGGCCTGCTGCTCTTCGCTGAAGCCCGCTTTGTGATCTGGCAGATGCAGCCAGCCGTGATGGCTGTGGATGTCGCCGCTTTCGCGCATCTTTTCAATCAGCAACAGTACCAGCGCTTCATCTTCCATTGGCAACGCCATACGTCGCAGACGTTCGCGCCCAGGGCCAGGTTCATCGCGATGTTGCTCATGATAAGTCGCTAATGTGTCGAGAATTTTCCGCTGCCAGCGGGCGGCAACCGGCGCATTCA  >  NC_000913/3758357‑3758630                                                                                                                                          |                                                                                                                                         aaCAGTGGCTCTGCTTTTTGCCAGATGGCCTGCTGCTCTTCGCTGAAGCCCGCTTTGTGATCTGGCAGATGCAGCCAGCCGTGATGGCTGTGGATGTCGCCGCTTTCGCGCATCTTTTCAATCAGCAACAGTACCAgtgc                                                                                                                                        >  2:1401581/1‑140 (MQ=255)     gTGGCTCTGCTTTTTGCCAGATGGCCTGCTGCTCTTCGCTGAAGCCCGCTTTGTGATCTGGCAGATGCAGCCAGCCGTGATGGCTGTGGATGTCGCCGCTTTCGCGCATCTTTTCAATCAGCAACAGTACCAGTGCTTCa                                                                                                                                    <  1:1401581/140‑1 (MQ=255)      tGGCTCTGCTTTTTGCCAGATGGCCTGCTGCTCTTCGCTGAAGCCCGCTTTGTGATCTGGCAGATGCAGCCAGCCGTGATGGCTGTGGATGTCGCCGCTTTCGCGCATCTTTTCAATCAGCAACAGTACCAGTGCTTCAt                                                                                                                                   >  1:548284/1‑140 (MQ=255)      tGGCTCTGCTTTTTGCCAGATGGCCTGCTGCTCTTCGCTGAAGCCCGCTTTGTGATCTGGCAGATGCAGCCAGCCGTGATGGCTGTGGATGTCGCCGCTTTCGCGCATCTTTTCAATCAGCAACAGTACCAGTGCTTCAt                                                                                                                                   >  2:330510/1‑140 (MQ=255)      tGGCTCTGCTTTTTGCCAGATGGCCTGCTGCTCTTCGCTGAAGCCCGCTTTGTGATCTGGCAGATGCAGCCAGCCGTGATGGCTGTGGATGTCGCCGCTTTCGCGCATCTTTTCAATCAGCAACAGTACCAGTGCTTCAt                                                                                                                                   >  2:534726/1‑140 (MQ=255)      tGGCTCTGCTTTTTGCCAGATGGCCTGCTGCTCTTCGCTGAAGCCCGCTTTGTGATCTGGCAGATGCAGCCAGCCGTGATGGCTGTGGATGTCGCCGCTTTCGCGCATCTTTTCAATCAGCAACAGTACCAGTGCTTCAt                                                                                                                                   >  2:236998/1‑140 (MQ=255)      tGGCTCTGCTTTTTGCCAGATGGCCTGCTGCTCTTCGCTGAAGCCCGCTTTGTGATCTGGCAGATGCAGCCAGCCGTGATGGCTGTGGATGTCGCCGCTTTCGCGCATCTTTTCAATCAGCAACAGTACCAGTGCTTCAt                                                                                                                                   >  2:1303324/1‑140 (MQ=255)      tGGCTCTGCTTTTTGCCAGATGGCCTGCTGCTCTTCGCTGAAGCCCGCTTTGTGATCTGGCAGATGCAGCCAGCCGTGATGGCTGTGGATGTCGCCGCTTTCGCGCATCTTTTCAATCAGCAACAGTACCAGTGCTTCAt                                                                                                                                   <  2:79531/140‑1 (MQ=255)      tGGCTCTGCTTTTTGCCAGATGGCCTGCTGCTCTTCGCTGAAGCCCGCTTTGTGATCTGGCAGATGCAGCCAGCCGTGATGGCTGTGGATGTCGCCGCTTTCGCGCATCTTTTCAATCAGCAACAGTACCAGTGCTTCAt                                                                                                                                   >  1:306650/1‑140 (MQ=255)      tGGCTCTGCTTTTTGCCAGATGGCCTGCTGCTCTTCGCTGAAGCCCGCTTTGTGATCTGGCAGATGCAGCCAGCCGTGATGGCTGTGGATGTCGCCGCTTTCGCGCATCTTTTCAATCAGCAACAGTACCAGTGCTTCAt                                                                                                                                   >  2:112093/1‑140 (MQ=255)      tGGCTCTGCTTTTTGCCAGATGGCCTGCTGCTCTTCGCTGAAGCCCGCTTTGTGATCTGGCAGATGCAGCCAGCCGTGATGGCTGTGGATGTCGCCGCTTTCGCGCATCTTTTCAATCAGCAACAGTACCAGTGCTTCAt                                                                                                                                   >  2:1077113/1‑140 (MQ=255)      tGGCTCTGCTTTTTGCCAGATGGCCTGCTGCTCTTCGCTGAAGCCCGCTTTGTGATCTGGCAGATGCAGCCAGCCGTGATGGCTGTGGATGTCGCCGCTTTCGCGCATCTTTTCAATCAGCAACAGTACCAGTGCTTCAt                                                                                                                                   <  1:86719/140‑1 (MQ=255)      tGGCTCTGCTTTTTGCCAGATGGCCTGCTGCTCTTCGCTGAAGCCCGCTTTGTGATCTGGCAGATGCAGCCAGCCGTGATGGCTGTGGATGTCGCCGCTTTCGCGCATCTTTTCAATCAGCAACAGTACCAGTGCTTCAt                                                                                                                                   >  2:93044/1‑140 (MQ=255)       ggCTCTGCTTTTTGCCAGATGGCCTGCTGCTCTTCGCTGAAGCCCGCTTTGTGATCTGGCAGATGCAGCCAGCCGTGATGGCTGTGGATGTCGCCGCTTTCGCGCATCTTTTCAATCAGCAACAGTACCAGTGCTTCATc                                                                                                                                  <  1:897839/140‑1 (MQ=255)         ctctGCTTTTTGCCAGATGGCCTGCTGCTCTTCGCTGAAGCCCGCTTTGTGATCTGGCAGATGCAGCCAGCCGTGATGGCTGTGGATGTCGCCGCTTTCGCGCATCTTTTCAATCAGCAACAGTACCAGTGCTTCATCtt                                                                                                                                >  2:810553/1‑140 (MQ=255)         ctctGCTTTTTGCCAGATGGCCTGCTGCTCTTCGCTGAAGCCCGCTTTGTGATCTGGCAGATGCAGCCAGCCGTGATGGCTGTGGATGTCGCCGCTTTCGCGCATCTTTTCAATCAGCAACAGTACCAGTGCTTCATCtt                                                                                                                                >  2:477242/1‑140 (MQ=255)            tGCTTTTTGCCAGATGGCCTGCTGCTCTTCGCTGAAGCCCGCTTTGTGATCTGGCAGATGCAGCCAGCCGTGATGGCTGTGGATGTCGCCGCTTTCGCGCATCTTTTCAATCAGCAACAGTACCAGTGCTTCATCTTCCa                                                                                                                             <  1:1120183/140‑1 (MQ=255)             gCTTTTTGCCAGATGGCCTGCTGCTCTTCGCTGAAGCCCGCTTTGTGATCTGGCAGATGCAGCCAGCCGTGATGGCTGTGGATGTCGCCGCTTTCGCGCATCTTTTCAATCAGCAACAGTACCAGTGCTTCATCTTCCAt                                                                                                                            <  2:605856/140‑1 (MQ=255)                ttttGCCAGATGGCCTGCTGCTCTTCGCTGAAGCCCGCTTTGTGATCTGGCAGATGCAGCCAGCCGTGATGGCTGTGGATGTCGCCGCTTTCGCGCATCTTTTCAATCAGCAACAGTACCAGTGCTTCATCTTCCATTgg                                                                                                                         <  1:825902/140‑1 (MQ=255)                ttttGCCAGATGGCCTGCTGCTCTTCGCTGAAGCCCGCTTTGTGATCTGGCAGATGCAGCCAGCCGTGATGGCTGTGGATGTCGCCGCTTTCGCGCATCTTTTCAATCAGCAACAGTACCAGTGCTTCATCTTCCATTgg                                                                                                                         <  2:805493/140‑1 (MQ=255)                ttttGCCAGATGGCCTGCTGCTCTTCGCTGAAGCCCGCTTTGTGATCTGGCAGATGCAGCCAGCCGTGATGGCTGTGGATGTCGCCGCTTTCGCGCATCTTTTCAATCAGCAACAGTACCAGTGCTTCATCTTCCATTgg                                                                                                                         <  1:1019580/140‑1 (MQ=255)                        gATGGCCTGCTGCTCTTCGCTGAAGCCCGCTTTGTGATCTGGCAGATGCAGCCAGCCGTGATGGCTGTGGATGTCGCCGCTTTCGCGCATCTTTTCAATCAGCAACAGTACCAGTGCTTCATCTTCCATTGGCa                                                                                                                       >  2:136717/1‑134 (MQ=255)                        gATGGCCTGCTGCTCTTCGCTGAAGCCCGCTTTGTGATCTGGCAGATGCAGCCAGCCGTGATGGCTGTGGATGTCGCCGCTTTCGCGCATCTTTTCAATCAGCAACAGTACCAGTGCTTCATCTTCCATTGGCa                                                                                                                       <  1:136717/134‑1 (MQ=255)                        gATGGCCTGCTGCTCTTCGCTGAAGCCCGCTTTGTGATCTGGCAGATGCAGCCAGCCGTGATGGCTGTGGATGTCGCCGCTTTCGCGCATCTTTTCAATCAGCAACAGTACCAGTGCTTCATCTTCCATTGGCAACGCCa                                                                                                                 <  1:765565/140‑1 (MQ=255)                        gATGGCCTGCTGCTCTTCGCTGAAGCCCGCTTTGTGATCTGGCAGATGCAGCCAGCCGTGATGGCTGTGGATGTCGCCACTTTCGCGCATCTTTTCAATCAGCAACAGTACCAGTGCTTCATCTTCCATTGGCAACGCCa                                                                                                                 >  2:799614/1‑140 (MQ=255)                          tGGCCTGCTGCTCTTCGCTGAAGCCCGCTTTGTGATCTGGCAGATGCAGCCAGCCGTGATGGCTGTGGATGTCGCCGCTTTCGCGCATCTTTTCAATCAGCAACAGTACCAGTGCTTCATCTTCCATTGGCAACGCCATa                                                                                                               <  1:594363/140‑1 (MQ=255)                             ccTGCTGCTCTTCGCTGAAGCCCGCTTTGTGATCTGGCAGATGCAGCCAGCCGTGATGGCTGTGGATGTCGCCGCTTTCGCGCATCTTTTCAATCAGCAACAGTACCAGTGCTTCATCTTCCATTGGCAACGCCATACGt                                                                                                            >  1:428483/1‑140 (MQ=255)                               tgctgcTCTTCGCTGAAGCCCGCTTTGTGATCTGGCAGATGCAGCCAGCCGTGATGGCTGTGGATGTCGCCGCTTTCGCGCATCTTTTCAATCAGCAACAGTACCAGTGCTTCATCTTCCATTGGCa                                                                                                                       <  1:779262/127‑1 (MQ=255)                               tgctgcTCTTCGCTGAAGCCCGCTTTGTGATCTGGCAGATGCAGCCAGCCGTGATGGCTGTGGATGTCGCCGCTTTCGCGCATCTTTTCAATCAGCAACAGTACCAGTGCTTCATCTTCCATTGGCa                                                                                                                       >  2:779262/1‑127 (MQ=255)                                         cGCTGAAGCCCGCTTTGTGATCTGGCAGATGCAGCCAGCCGTGATGGCTGTGGATGTCGCCGCTTTCGCGCATCTTTTCAATCAGCAACAGTACCAGTGCTTCATCTTCCATTGGCAACGCCATACGTCGCAGACGTTcg                                                                                                >  1:1119858/1‑140 (MQ=255)                                         cGCTGAAGCCCGCTTTGTGATCTGGCAGATGCAGCCAGCCGTGATGGCTGTGGATGTCGCCGCTTTCGCGCATCTTTTCAATCAGCAACAGTACCAGTGCTTCATCTTCCATTGGCAACGCCATACGTCGCAGACGTTcg                                                                                                <  2:628246/140‑1 (MQ=255)                                             gAAGCCCGCTTTGTGATCTGGCAGATGCAGCCAGCCGTGATGGCTGTGGATGTCGCCGCTTTCGCGCATCTTTTCAATCAGCAACAGTACCAGTGCTTCATCTTCCATTGGCAACGCCATACGTCGCAGACGTTCGCGcc                                                                                            <  1:286222/140‑1 (MQ=255)                                             gAAGCCCGCTTTGTGATCTGGCAGATGCAGCCAGCCGTGATGGCTGTGGATGTCGCCGCTTTCGCGCATCTTTTCAATCAGCAACAGTACCAGTGCTTCATCTTCCATTGGCAACGCCATACGTCGCAGACGTTCGCGcc                                                                                            <  1:1303324/140‑1 (MQ=255)                                             gAAGCCCGCTTTGTGATCTGGCAGATGCAGCCAGCCGTGATGGCTGTGGATGTCGCCGCTTTCGCGCATCTTTTCAATCAGCAACAGTACCAGTGCTTCATCTTCCATTGGCAACGCCATACGTCGCAGACGTTCGCGcc                                                                                            <  1:945159/140‑1 (MQ=255)                                             gAAGCCCGCTTTGTGATCTGGCAGATGCAGCCAGCCGTGATGGCTGTGGATGTCGCCGCTTTCGCGCATCTTTTCAATCAGCAACAGTACCAGTGCTTCATCTTCCATTGGCAACGCCATACGTCGCAGACGTTCGCGcc                                                                                            <  1:477242/140‑1 (MQ=255)                                              aaGCCCGCTTTGTGATCTGGCAGATGCAGCCAGCCGTGATGGCTGTGGATGTCGCCGCTTTCGCGCATCTTTTCAATCAGCAACAGTACCAGTGCTTCATCTTCCATTGGCAACGCCATACGTCGCAGACGTTCGCGccc                                                                                           <  1:1110921/140‑1 (MQ=255)                                               aGCCCGCTTTGTGATCTGGCAGATGCAGCCAGCCGTGATGGCTGTGGATGTCGCCGCTTTCGCGCATCTTTTCAATCAGCAACAGTACCAGTGCTTCATCTTCCATTGGCAACGCCATACGTCGCAGACGTTCGCGCCCa                                                                                          <  2:280481/140‑1 (MQ=255)                                               aGCCCGCTTTGTGATCTGGCAGATGCAGCCAGCCGTGATGGCTGTGGATGTCGCCGCTTTCGCGCATCTTTTCAATCAGCAACAGTACCAGTGCTTCATCTTCCATTGGCAACGCCATACGTCGCAGACGTTCGCGCCCa                                                                                          <  2:548284/140‑1 (MQ=255)                                               aGCCCGCTTTGTGATCTGGCAGATGCAGCCAGCCGTGATGGCTGTGGATGTCGCCGCTTTCGCGCATCTTTTCAATCAGCAACAGTACCAGTGCTTCATCTTCCATTGGCAACGCCATACGTCGCAGACGTTCGCGCCCa                                                                                          <  2:657335/140‑1 (MQ=255)                                                   cGCTTTGTGATCTGGCAGATGCAGCCAGCCGTGATGGCTGTGGATGTCGCCGCTTTCGCGCATCTTTTCAATCAGCAACAGTACCAGTGCTTCATCTTCCATTGGCAACGCCATACGTCGCAGACGTTCGCGCCCAGGGc                                                                                      >  1:1214525/1‑140 (MQ=255)                                                   cGCTTTGTGATCTGGCAGATGCAGCCAGCCGTGATGGCTGTGGATGTCGCCGCTTTCGCGCATCTTTTCAATCAGCAACAGTACCAGTGCTTCATCTTCCATTGGCAACGCCATACGTCGCAGACGTTCGCGCCCAGGGc                                                                                      >  1:1075601/1‑140 (MQ=255)                                                    gCTTTGTGATCTGGCAGATGCAGCCAGCCGTGATGGCTGTGGATGTCGCCGCTTTCGCGCATCTTTTCAATCAGCAACAGTACCAGTGCTTCATCTTCCATTGGCAACGCCATACGTCGCAGACGTTCGCGCCCAGGGcc                                                                                     <  1:810553/140‑1 (MQ=255)                                                              cTGGCAGATGCAGCCAGCCGTGATGGCTGTGGATGTCGCCGCTTTCGCGCATCTTTTCAATCAGCAACAGTACCAGTGCTTCATCTTCCATTGGCAACGCCATACGTCGCAGACGTTCGCGCCCAGGGCCAGGTTCATcg                                                                           <  2:1191867/140‑1 (MQ=255)                                                                ggCAGATGCAGCCAGCCGTGATGGCTGTGGATGTCGCCGCTTTCGCGCATCTTTTCAATCAGCAACAGTGCCAGTGCTTCATCTTCCATTGGCAACGCCATACGTCGCAGACGTTCGCGCCCAGGGCCAGGTTCATcgcg                                                                         >  2:74870/1‑140 (MQ=255)                                                                ggCAGATGCAGCCAGCCGTGATGGCTGTGGATGTCGCCGCTTTCGCGCATCTTTTCAATCAGCAACAGTACCAGTGCTTCATCTTCCATTGGCAACGCCATACGTCGCAGACGTTCGCGCCCAGGGCCAGGTTCATcgcg                                                                         >  1:935663/1‑140 (MQ=255)                                                                 gCAGATGCAGCCAGCCGTGATGGCTGTGGATGTCGCCGCTTTCGCGCATCTTTTCAATCAGCAACAGTACCAGTGCTTCATCTTCCATTGGCAACGCCATACGTCGCAGACGTTCGCGCCCAGGGCCAGGTTCATCGCGa                                                                        <  2:428483/140‑1 (MQ=255)                                                                  cAGATGCAGCCAGCCGTGATGGCTGTGGATGTCGCCGCTTTCGCGCATCTTTTCAATCAGCAACAGTACCAGTGCTTCATCTTCCATTGGCAACGCCATACGTCGCAGACGTTCGCGCCCAGGGCCAGGTTCATCGCGAt                                                                       <  2:463629/140‑1 (MQ=255)                                                                   aGATGCAGCCAGCCGTGATGGCTGTGGATGTCGCCGCTTTCGCGCATCTTTTCAATCAGCAACAGTACCAGTGCTTCATCTTCCATTGGCAACGCCATACGTCGCAGACGTTCGCGCCCAGGGCCAGGTTCATCGCGATg                                                                      <  2:1149807/140‑1 (MQ=255)                                                                   aGATGCAGCCAGCCGTGATGGCTGTGGATGTCGCCGCTTTCGCGCATCTTTTCAATCAGCAACAGTACCAGTGCTTCATCTTCCATTGGCAACGCCATACGTCGCAGACGTTCGCGCCCAGGGCCAGGTTCATCGCGATg                                                                      <  2:383998/140‑1 (MQ=255)                                                                      tGCAGCCAGCCGTGATGGCTGTGGATGTCGCCGCTTTCGCGCATCTTTTCAATCAGCAACAGTACCAGTGCTTCATCTTCCATTGGCAACGCCATACGTCGCAGACGTTCGCGCCCAGGGCCAGGTTCATCGCGATGTTg                                                                   <  1:1077113/140‑1 (MQ=255)                                                                         agccagccGTGATGGCTGTGGATGTCGCCGCTTTCGCGCATCTTTTCAATCAGCAACAGTACCAGTGCTTCATCTTCCATTGGCAACGCCATACGTCGCAGACGTTCGCGCCCAGGGCCAGGTTCATCGCGATGTTGCTc                                                                >  2:84975/1‑140 (MQ=255)                                                                           ccagccGTGATGGCTGTGGATGTCGCCGCTTTCGCGCATCTTTTCAATCAGCAACAGTACCAGTGCTTCATCTTCCATTGGCAACGCCATACGTCGCAGACGTTCGCGCCCAGGGCCAGGTTCATCGCGATGTTGCTCAt                                                              >  1:502481/1‑140 (MQ=255)                                                                           ccagccGTGATGGCTGTGGATGTCGCCGCTTTCGCGCATCTTTTCAATCAGCAACAGTACCAGTGCTTCATCTTCCATTGGCAACGCCATACGTCGCAGACGTTCGCGCCCAGGGCCAGGTTCATCGCGATGTTGCTCAt                                                              >  1:1270376/1‑140 (MQ=255)                                                                              gccGTGATGGCTGTGGATGTCGCCGCTTTCGCGCATCTTTTCAATCAGCAACAGTACCAGTGCTTCATCTTCCATTGGCAACGCCATACGTCGCAGACGTTCGCGCCCAGGGCCAGGTTCATCGCGATGTTGCTCATGAt                                                           <  2:474029/140‑1 (MQ=255)                                                                               ccGTGATGGCTGTGGATGTCGCCGCTTTCGCGCATCTTTTCAATCAGCAACAGTACCAGTGCTTCATCTTCCATTGGCAACGCCATACGTCGCAGACGTTCGCGCCCAGGGCCAGGTTCATCGCGATGTTGCTCATGATa                                                          <  1:1235633/140‑1 (MQ=255)                                                                               ccGTGATGGCTGTGGATGTCGCCGCTTTCGCGCATCTTTTCAATCAGCAACAGTACCAGTGCTTCATCTTCCATTGGCAACGCCATACGTCGCAGACGTTCGCGCCCAGGGCCAGGTTCATCGCGATGTTGCTCATGATa                                                          <  1:84975/140‑1 (MQ=255)                                                                                  tGATGGCTGTGGATGTCGCCGCTTTCGCGCATCTTTTCAATCAGCAACAGTACCAGTGCTTCATCTTCCATTGGCAACGCCATACGTCGCAGACGTTCGCGCCCAGGGCCAGGTTCATCGCGATGTTGCTCATGATAAGt                                                       <  2:306650/140‑1 (MQ=255)                                                                                      ggCTGTGGATGTCGCCGCTTTCGCGCATCTTTTCAATCAGCAACAGTACCAGTGCTTCATCTTCCATTGGCAACGCCATACGTCGCAGACGTTCGCGCCCAGGGCCAGGTTCATCGCGATGTTGCTCATGATAAGTCGCt                                                   <  2:513802/140‑1 (MQ=255)                                                                                       gCTGTGGATGTCGCCACTTTCGCGCATCTTTTCAATCAGCAACAGTACCAGTGCTTCATCTTCCATTGGCAACGCCATACGTCGCAGACGTTCGCGCCCAGGGCCAGGTTCATCGCGATGTTGCTCATGATAAGTCGCTa                                                  <  1:799614/140‑1 (MQ=255)                                                                                         tgtgGATGTCGCCGCTTTCGCGCATCTTTTCAATCAGCAACAGTACCAGTGCTTCATCTTCCATTGGCAACGCCATACGTCGCAGACGTTCGCGCCCAGGGCCAGGTTCATCGCGATGTTGCTCATGa                                                            <  1:81521/128‑1 (MQ=255)                                                                                         tgtgGATGTCGCCGCTTTCGCGCATCTTTTCAATCAGCAACAGTACCAGTGCTTCATCTTCCATTGGCAACGCCATACGTCGCAGACGTTCGCGCCCAGGGCCAGGTTCATCGCGATGTTGCTCATGa                                                            >  2:81521/1‑128 (MQ=255)                                                                                         tgtgGATGTCGCCGCTTTCGCGCATCTTTTCAATCAGCAACAGTACCAGTGCTTCATCTTCCATTGGCAACGCCATACGTCGCAGACGTTCGCGCCCAGGGCCAGGTTCATCGCGATGTTGCTCATGATAAGTCGCTAAt                                                >  2:965654/1‑140 (MQ=255)                                                                                           tgGATGTCGCCGCTTTCGCGCATCTTTTCAATCAGCAACAGTACCAGTGCTTCATCTTCCATTGGCAACGCCATACGTCGCAGACGTTCGCGCCCAGGGCCAGGTTCATCGCGATGTTGCTCATGATAAGt                                                       <  1:47262/131‑1 (MQ=255)                                                                                           tgGATGTCGCCGCTTTCGCGCATCTTTTCAATCAGCAACAGTACCAGTGCTTCATCTTCCATTGGCAACGCCATACGTCGCAGACGTTCGCGCCCAGGGCCAGGTTCATCGCGATGTTGCTCATGATAAGt                                                       >  2:47262/1‑131 (MQ=255)                                                                                                  cgccgcTTTCGCGCATCTTTTCAATCAGCAACAGTACCAGTGCTTCATCTTCCATTGGCAACGCCATACGTCGCAGACGTTCGCGCCCAGGGCCAGGTTCATCGCGATGTTGCTCATGATAAGTCGCTAATGTGTCgaga                                       >  1:102121/1‑140 (MQ=255)                                                                                                  cgccgcTTTCGCGCATCTTTTCAATCAGCAACAGTACCAGTGCTTCATCTTCCATTGGCAACGCCATACGTCGCAGACGTTCGCGCCCAGGGCCAGGTTCATCGCGATGTTGCTCATGATAAGTCGCTAATGTGTCgaga                                       >  2:648221/1‑140 (MQ=255)                                                                                                          tCGCGCATCTTTTCAATCAGCAACAGTACCAGTGCTTCATCTTCCATTGGCAACGCCATACGTCGCAGACGTTCGCGCCCAGGGCCAGGTTCATCGCGATGTTGCTCATGATAAGTCGCTAATGTGTCGAGAATTTTCCg                               <  2:1214525/140‑1 (MQ=255)                                                                                                                 tCTTTTCAATCAGCAACAGTACCAGTGCTTCATCTTCCATTGGCAACGCCATACGTCGCAGACGTTCGCGCCCAGGGCCAGGTTCATCGCGATGTTGCTCATGATAAGTCGCTAATGTGTCGAGAATTTTCCGCTGCCAg                        <  2:1075601/140‑1 (MQ=255)                                                                                                                 tCTTTTCAATCAGCAACAGTACCAGTGCTTCATCTTCCATTGGCAACGCCATACGTCGCAGACGTTCGCGCCCAGGGCCAGGTTCATCGCGATGTTGCTCATGATAAGTCGCTAATGTGTCGAGAATTTTCCGCTGCCAg                        <  1:965654/140‑1 (MQ=255)                                                                                                                           cAGCAACAGTGCCAGTGCTTCATCTTCCATTGGCAACGCCATACGTCGCAGACGTTCGCGCCCAGGGCCAGGTTCATCGCGATGTTGCTCATGATAAGTCGCTAATGTGTCGAGAATTTTCCGCTGCCAGCGGGCGGCaa              <  1:74870/140‑1 (MQ=255)                                                                                                                            aGCAACAGTACCAGTGCTTCATCTTCCATTGGCAACGCCATACGTCGCAGACGTTCGCGCCCAGGGCCAGGTTCATCGCGATGTTGCTCATGATAAGTCGCTAATGTGTCGAGAATTTTCCGCTGCCAGCGGGCGGCAAc             >  1:397322/1‑140 (MQ=255)                                                                                                                            aGCAACAGTACCAGTGCTTCATCTTCCATTGGCAACGCCATACGTCGCAGACGTTCGCGCCCAGGGCCAGGTTCATCGCGATGTTGCTCATGATAAGTCGCTAATGTGTCGAGAATTTTCCGCTGCCAGCGGGCGGCAAc             >  2:1134478/1‑140 (MQ=255)                                                                                                                            aGCAACAGTACCAGTGCTTCATCTTCCATTGGCAACGCCATACGTCGCAGACGTTCGCGCCCAGGGCCAGGTTCATCGCGATGTTACTCATGATAAGTCGCTAATGTGTCGAGAATTTTCCGCTGCCAGCGGGCGGCAAc             >  1:616600/1‑140 (MQ=255)                                                                                                                             gCAACAGTACCAGTGCTTCATCTTCCATTGGCAACGCCATACGTCGCAGACGTTCGCGCCCAGGGCCAGGTTCATCGCGATGTTGCTCATGATAAGTCGCTAATGTGTCGAGAATTTTCCGCTGCCAGCGGGCGGCAAcc            >  1:386743/1‑140 (MQ=255)                                                                                                                              cAACAGTACCAGTGCTTCATCTTCCATTGGCAACGCCATACGTCGCAGACGTTCGCGCCCAGGGCCAGGTTCATCGCGATGTTGCTCATGATAAGTCGCTAATGTGTCGAGAATTTTCCGCTGCCAg                        >  1:1064984/1‑127 (MQ=255)                                                                                                                              cAACAGTACCAGTGCTTCATCTTCCATTGGCAACGCCATACGTCGCAGACGTTCGCGCCCAGGGCCAGGTTCATCGCGATGTTGCTCATGATAAGTCGCTAATGTGTCGAGAATTTTCCGCTGCCAg                        <  2:1064984/127‑1 (MQ=255)                                                                                                                              cAACAGTACCAGTGCTTCATCTTCCATTGGCAACGCCATACGTCGCAGACGTTCGCGCCCAGGGCCAGGTTCATCGCGATGTTGCTCATGATAAGTCGCTAATGTGTCGAGAATTTTCCGCTGCCAGCGGGCGGCAACCg           >  2:612535/1‑140 (MQ=255)                                                                                                                              cAACAGTACCAGTGCTTCATCTTCCATTGGCAACGCCATACGTCGCAGACGTTCGCGCCCAGGGCCAGGTTCATCGCGATGTTGCTCATGATAAGTCGCTAATGTGTCGAGAATTTTCCGCTGCCAGCGGGCGGCAACCg           >  2:259065/1‑140 (MQ=255)                                                                                                                              cAACAGTACCAGTGCTTCATCTTCCATTGGCAACGCCATACGTCGCAGACGTTCGCGCCCAGGGCCAGGTTCATCGCGATGTTGCTCATGATAAGTCGCTAATGTGTCGAGAATTTTCCGCTGCCAGCGGGCGGCAACCg           >  2:422696/1‑140 (MQ=255)                                                                                                                              cAACAGTACCAGTGCTTCATCTTCCATTGGCAACGCCATACGTCGCAGACGTTCGCGCCCAGGGCCAGGTTCATCGCGATGTTGCTCATGATAAGTCGCTAATGTGTCGAGAATTTTCCGCTGCCAGCGGGCGGCAACCg           >  2:477048/1‑140 (MQ=255)                                                                                                                               aaCAGTACCAGTGCTTCATCTTCCATTGGCAACGCCATACGTCGCAGACGCTCGCGCCCAGGGCCAGGTTCATCGCGATGTTGCTCATGATAAGTCGCTAATGTGTCGAGAATTTTCCGCTGCCAGCGGGCGGCAACCgg          <  2:1270376/140‑1 (MQ=255)                                                                                                                                    tACCAGTGCTTCATCTTCCATTGGCAACGCCATACGTCGCAGACGTTCGCGCCCAGGGCCAGGTTCATCGCGATGTTGCTCATGATAAGTCGCTAATGTGTCGAGAATTTTCCGCTGCCAGCGGGCGGCAACCGGCGCAt     <  1:162560/140‑1 (MQ=255)                                                                                                                                      ccAGTGCTTCATCTTCCATTGGCAACGCCATACGTCGCAGACGTTCGCGCCCAGGGCCAGGTTCATCGCGATGTTGCTCATGATAAGTCGCTAATGTGTCGAGAATTTTCCGCTGCCAGCGGGCGGCAACCGGCGCATTc   >  1:581314/1‑140 (MQ=255)                                                                                                                                      ccAGTGCTTCATCTTCCATTGGCAACGCCATACGTCGCAGACGTTCGCGCCCAGGGCCAGGTTCATCGCGATGTTGCTCATGATAAGTCGCTAATGTGTCGAGAATTTTCCGCTGCCAGCGGGCGGCAACCGGCGCATTc   >  1:523074/1‑140 (MQ=255)                                                                                                                                       cAGTGCTTCATCTTCCATTGGCAACGCCATACGTCGCAGACGTTCGCGCCCAGGGCCAGGTTCATCGCGATGTTGCTCATGATAAGTCGCTAATGTGTCGAGAATTTTCCGCTGCCAGCGGGCGGCAACCGGCGCATTca  >  1:505553/1‑140 (MQ=255)                                                                                                                                          |                                                                                                                                         AACAGTGGCTCTGCTTTTTGCCAGATGGCCTGCTGCTCTTCGCTGAAGCCCGCTTTGTGATCTGGCAGATGCAGCCAGCCGTGATGGCTGTGGATGTCGCCGCTTTCGCGCATCTTTTCAATCAGCAACAGTACCAGCGCTTCATCTTCCATTGGCAACGCCATACGTCGCAGACGTTCGCGCCCAGGGCCAGGTTCATCGCGATGTTGCTCATGATAAGTCGCTAATGTGTCGAGAATTTTCCGCTGCCAGCGGGCGGCAACCGGCGCATTCA  >  NC_000913/3758357‑3758630

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑