Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,612,587	C→T	G59G (GGC→GGT)	yjjU →	putative patatin‑like phospholipase YjjU

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,612,587	0	C	T	100.0%	201.4 / NA	58	G59G (GGC→GGT)	yjjU	putative patatin‑like phospholipase YjjU
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (28/30);  total (28/30)

GGCCGTTCCAGCCTGGACGAATAGCTCTGGTGTGCGAAGGCGGCGGACAGCGTGGAATTTTCACGGCTGGCGTGCTGGATGAGTTTATGCGCGCGCAGTTTAATCCTTTCGATCTTTATCTCGGCACATCTGCCGGGGCGCAGAACCTCTCGGCGTTTATCTGCAATCAGCCCGGTTACGCGCGCAAAGTCATCATGCGCTATACCACAAAACGCGAATTTTTCGATCCATTGCGCTTTGTCCGTGGAGGAAATCTTAT  >  NC_000913/4612463‑4612721                                                                                                                             |                                                                                                                                       ggCCGTTCCAGCCTGGACGAATAGCTCTGGTGTGCGAAGGCGGCGGACAGCGTGGAATTTTCACGGCTGGCGTGCTGGATGAGTTTATGCGCGCGCAGTTTAATCCTTTCGATCTTTATCTCGGTACATCTGCCg                                                                                                                              <  1:474248/135‑1 (MQ=255) ggCCGTTCCAGCCTGGACGAATAGCTCTGGTGTGCGAAGGCGGCGGACAGCGTGGAATTTTCACGGCTGGCGTGCTGGATGAGTTTATGCGCGCGCAGTTTAATCCTTTCGATCTTTATCTCGGTACATCTGCCg                                                                                                                              >  2:474248/1‑135 (MQ=255) ggCCGTTCCAGCCTGGACGAATAGCTCTGGTGTGCGAAGGCGGCGGACAGCGTGGAATTTTCACGGCTGGCGTGCTGGATGAGTTTATGCGCGCGCAGTTTAATCCTTTCGATCTTTATCTCGGTACATCTGCCGGGgcg                                                                                                                         >  2:1109487/1‑140 (MQ=255)    cGTTCCAGCCTGGACGAATAGCTCTGGTGTGCGAAGGCGGCGGACAGCGTGGAATTTTCACGGCTGGCGTGCTGGATGAGTTTATGCGCGCGCAGTTTAATCCTTTCGATCTTTATCTCGGTACATCTGCCGGGGCGCAg                                                                                                                      <  2:732764/140‑1 (MQ=255)      ttCCAGCCTGGACGAATAGCTCTGGTGTGTGAAGGCGGCGGACAGCGTGGAATTTTCACGGCTGGCGTGCTGGATGAGTTTATGCGCGCGCAGTTTAATCCTTTCGATCTTTATCTCGGTACATCTGCCGGGGCGCAGaa                                                                                                                    <  1:1221384/140‑1 (MQ=255)      ttCCAGCCTGGACGAATAGCTCTGGTGTGCGAAGGCGGCGGACAGCGTGGAATTTTCACGGCTGGCGTGCTGGATGAGTTTATGCGCGCGCAGTTTAATCCTTTCGATCTTTATCTCGGTACATCTGCCgg                                                                                                                             >  1:1070107/1‑131 (MQ=255)      ttCCAGCCTGGACGAATAGCTCTGGTGTGCGAAGGCGGCGGACAGCGTGGAATTTTCACGGCTGGCGTGCTGGATGAGTTTATGCGCGCGCAGTTTAATCCTTTCGATCTTTATCTCGGTACATCTGCCgg                                                                                                                             <  2:1070107/131‑1 (MQ=255)      ttCCAGCCTGGACGAATAGCTCTGGTGTGCGAAGGCGGCGGACAGCGTGGAATTTTCACGGCTGGCGTGCTGGATGAGTTTATGCGCGCGCAGTTTAATCCTTTCGATCTTTATCTCGGTACATCTGCCGGGGCGCAGaa                                                                                                                    <  2:289317/140‑1 (MQ=255)           gCCTGGACGAATAGCTCTGGTGTGCGAAGGCGGCGGACAGCGTGGAATTTTCACGGCTGGCGTGCTGGATGAGTTTATGCGCGCGCAGTTTAATCCTTTCGATCTTTATCTCGGTACATCTGCCGGGGCGCAGAACctct                                                                                                               <  1:108797/140‑1 (MQ=255)            ccTGGACGAATAGCTCTGGTGTGCGAAGGCGGCGGACAGCGTGGAATTTTCACGGCTGGCGTGCTGGATGAGTTTATGCGCGCGCAGTTTAATCCTTTCGATCTTTATCTCGGTACATCTGCCGGGGCGCAGAACCtctc                                                                                                              >  1:707334/1‑140 (MQ=255)            ccTGGACGAATAGCTCTGGTGTGCGAAGGCGGCGGACAGCGTGGAATTTTCACGGCTGGCGTGCTGGATGAGTTTATGCGCGCGCAGTTTAATCCTTTCGATCTTTATCTCGGTACATCTGCCGGGGCGCAGAACCtctc                                                                                                              >  1:601292/1‑140 (MQ=255)               ggACGAATAGCTCTGGTGTGCGAAGGCGGCGGACAGCGTGGAATTTTCACGGCTGGCGTGCTGGATGAGTTTATGCGCGCGCAGTTTAATCCTTTCGATCTTTATCTCGGTACATCTGCCGGGGCGCAGAACCTCTCGGc                                                                                                           >  1:1055138/1‑140 (MQ=255)                 aCGAATAGCTCTGGTGTGCGAAGGCGGCGGACAGCGTGGAATTTTCACGGCTGGCGTGCTGGATGAGTTTATGCGCGCGCAGTTTAATCCTTTCGATCTTTATCTCGGTACATCTGCCGGGGCGCAGAACCTCTCGGCGt                                                                                                         >  2:916756/1‑140 (MQ=255)                  cGAATAGCTCTGGTGTGCGAAGGCGGCGGACAGCGTGGAATTTTCACGGCTGGCGTGCTGGATGAGTTTATGCGCGCGCAGTTTAATCCTTTCGATCTTTATCTCGGTACATCTGCCGGGGCGCAGAACCTCTCGGCGtt                                                                                                        <  2:601292/140‑1 (MQ=255)                     aTAGCTCTGGTGTGCGAAGGCGGCGGACAGCGTGGAATTTTCACGGCTGGCGTGCTGGATGAGTTTATGCGCGCGCAGTTTAATCCTTTCGATCTTTATCTCGGTACATCTGCCGGGGCGCAGAACCTCTCGGCGTTTAt                                                                                                     >  2:726264/1‑140 (MQ=255)                              gtgtGCGAAGGCGGCGGACAGCGTGGAATTTTCACGGCTGGCGTGCTGGATGAGTTTATGCGCGCGCAGTTTAATCCTTTCGATCTTTATCTCGGTACATCTGCCGGGGCGCAGAACCTCTCGGCGTTTATCTGCAATCa                                                                                            >  1:843081/1‑140 (MQ=255)                                gtgCGAAGGCGGCGGACAGCGTGGAATTTTCACGGCTGGCGTGCTGGATGAGTTTATGCGCGCGCAGTTTAATCCTTTCGATCTTTATCTCGGTACATCTGCCGGGGCGCAGAACCTCTCGGCGTTTATCTGCAAt                                                                                              >  1:1176578/1‑136 (MQ=255)                                gtgCGAAGGCGGCGGACAGCGTGGAATTTTCACGGCTGGCGTGCTGGATGAGTTTATGCGCGCGCAGTTTAATCCTTTCGATCTTTATCTCGGTACATCTGCCGGGGCGCAGAACCTCTCGGCGTTTATCTGCAAt                                                                                              <  2:1176578/136‑1 (MQ=255)                                 tgCGAAGGCGGCGGACAGCGTGGAATTTTCACGGCTGGCGTGCTGGATGAGTTTATGCGCGCGCAGTTTAATCCTTTCGATCTTTATCTCGGTACATCTGCCGGGGCGCAGAACCTCTCGGCGTTTATCTGCAATCAGcc                                                                                         >  1:285682/1‑140 (MQ=255)                                 tgCGAAGGCGGCGGACAGCGTGGAATTTTCACGGCTGGCGTGCTGGATGAGTTTATGCGCGCGCAGTTTAATCCTTTCGATCTTTATCTCGGTACATCTGCCGGGGCGCAGAACCTCTCGGCGTTTATCTGCAATCAGcc                                                                                         >  1:1219720/1‑140 (MQ=255)                                     aaGGCGGCGGACAGCGTGGAATTTTCACGGCTGGCGTGCTGGATGAGTTTATGCGCGCGCAGTTTAATCCTTTCGATCTTTATCTCGGTACATCTGCCGGGGCGCAGAACCTCTCGGCGTTTATCTGCAATCAGCCCGGt                                                                                     <  1:916756/140‑1 (MQ=255)                                      aGGCGGCGGACAGCGTGGAATTTTCACGGCTGGCGTGCTGGATGAGTTTATGCGCGCGCAGTTTAATCCTTTCGATCTTTATCTCGGTACATCTGCCGGGGCGCAGAACCTCTCGGCGTTTATCTGCAATCAGCCCGGtt                                                                                    >  1:1288047/1‑140 (MQ=255)                                               aCAGCGTGGAATTTTCACGGCTGGCGTGCTGGATGAGTTTATGCGCGCGCAGTTTAATCCTTTCGATCTTTATCTCGGTACATCTGCCGGGGCGCAGAACCTCTCGGCGTTTATCTGCAATCAGCCCGGTTACGCGCGCa                                                                           <  1:1221369/140‑1 (MQ=255)                                                   cGTGGAATTTTCACGGCTGGCGTGCTGGATGAGTTTATGCGCGCGCAGTTTAATCCTTTCGATCTTTATCTCGGTACATCTGCCGGGGCGCAGAACCTCTCGGCGTTTATCTGCAATCAGCCCGGTTACGCGCGCAAAGt                                                                       >  1:523232/1‑140 (MQ=255)                                                     tGGAATTTTCACGGCTGGCGTGCTGGATGAGTTTATGCGCGCGCAGTTTAATCCTTTCGATCTTTATCTCGGTACATCTGCCGGGGCGCAGAACCTCTCGGCGTTTATCTGCAATCAGCCCGGTTACGCGCGCAAAGtca                                                                     <  2:1083131/140‑1 (MQ=255)                                                       gAATTTTCACGGCTGGCGTGCTGGATGAGTTTATGCGCGCGCAGTTTAATCCTTTCGATCTTTATCTCGGTACATCTGCCGGGGCGCAGAACCTCTCGGCGTTTATCTGCAATCAGCCCGGTTACGCGCGCAAAGtcatc                                                                   <  2:1055138/140‑1 (MQ=255)                                                       gAATTTTCACGGCTGGCGTGCTGGATGAGTTTATGCGCGCGCAGTTTAATCCTTTCGATCTTTATCTCGGTACATCTGCCGGGGCGCAGAACCTCTCGGCGTTTATCTGCAATCAGCCCGGTTACGCGCGCAAAGtcatc                                                                   <  1:1311337/140‑1 (MQ=255)                                                         aTTTTCACGGCTGGCGTGCTGGATGAGTTTATGCGCGCGCAGTTTAATCCTTTCGATCTTTATCTCGGTACATCTGCCGGGGCGCAGAACCTCTCGGCGTTTATCTGCAATCAGCCCGGTTAcgc                                                                                <  1:1190564/125‑1 (MQ=255)                                                         aTTTTCACGGCTGGCGTGCTGGATGAGTTTATGCGCGCGCAGTTTAATCCTTTCGATCTTTATCTCGGTACATCTGCCGGGGCGCAGAACCTCTCGGCGTTTATCTGCAATCAGCCCGGTTAcgc                                                                                >  2:1190564/1‑125 (MQ=255)                                                          ttttCACGGCTGGCGTGCTGGATGAGTTTATGCGCGCGCAGTTTAATCCTTTCGATCTTTATCTCGGTACATCTGCCGGGGCGCAGAACCTCTCGGCGTTTATCTGCAATCAGCCCGGTTACGCGCGCAAAGTCATCATg                                                                <  1:639816/140‑1 (MQ=255)                                                          ttttCACGGCTGGCGTGCTGGATGAGTTTATGCGCGCGCAGTTTAATCCTTTCGATCTTTATCTCGGTACATCTGCCGGGGCGCAGAACCTCTCGGCGTTTATCTGCAATCAGCCCGGTTACGCGCGCAAAGTCATCATg                                                                <  2:498011/140‑1 (MQ=255)                                                          ttttCACGGCTGGCGTGCTGGATGAGTTTATGCGCGCGCAGTTTAATCCTTTCGATCTTTATCTCGGTACATCTGCCGGGGCGCAGAACCTCTCGGCGTTTATCTGCAATCAGCCCGGTTACGCGCGCAAAGTCATCATg                                                                <  2:202177/140‑1 (MQ=255)                                                          ttttCACGGCTGGCGTGCTGGATGAGTTTATGCGCGCGCAGTTTAATCCTTTCGATCTTTATCTCGGTACATCTGCCGGGGCGCAGAACCTCTCGGCGTTTATCTGCAATCAGCCCGGTTACGCGCGCAAAGTCATCATg                                                                <  2:12958/140‑1 (MQ=255)                                                            ttCACGGCTGGCGTGCTGGATGAGTTTATGCGCGCGCAGTTTAATCCTTTCGATCTTTATCTCGGTACATCTGCCGGGGCGCAGAACCTCTCGGCGTTTATCTGCAATCAGCCCGGTTACGCGCGCAAAGTCATCATgcg                                                              <  1:726264/140‑1 (MQ=255)                                                            ttCACGGCTGGCGTGCTGGATGAGTTTATGCGCGCGCAGTTTAATCCTTTCGATCTTTATCTCGGTACATCTGCCGGGGCGCAGAACCTCTCGGCGTTTATCTGCAATCAGCCCGGTTACGCGCGCAAAGTCATCATgcg                                                              <  2:707334/140‑1 (MQ=255)                                                            ttCACGGCTGGCGTGCTGGATGAGTTTATGCGCGCGCAGTTTAATCCTTTCGATCTTTATCTCGGTACATCTGCCGGGGCGCAGAACCTCTCGGCGTTTATCTGCAATCAGCCCGGTTACGCGCGCAAAGTCATCATgcg                                                              <  2:285682/140‑1 (MQ=255)                                                            ttCACGGCTGGCGTGCTGGATGAGTTTATGCGCGCGCAGTTTAATCCTTTCGATCTTTATCTCGGTACATCTGCCGGGGCGCAGAACCTCTCGGCGTTTATCTGCAATCAGCCCGGTTACGCGCGCAAAGTCATCATgcg                                                              <  2:1219720/140‑1 (MQ=255)                                                               aCGGCTGGCGTGCTGGATGAGTTTATGCGCGCGCAGTTTAATCCTTTCGATCTTTATCTCGGTACATCTGCCGGGGCGCAGAACCTCTCGGCGTTTATCTGCAATCAGCCCGGTTACGCGCGCAAAGTCATCATGCGCta                                                           <  2:523232/140‑1 (MQ=255)                                                                               aTGAGTTTATGCGCGCGCAGTTTAATCCTTTCGATCTTTATCTCGGTACATCTGCCGGGGCGCAGAACCTCTCGGCGTTTATCTGCAATCAGCCCGGTTACGCGCGCAAAGTCATCATGCGCTATACCACAAAACGCGaa                                           <  2:42404/140‑1 (MQ=255)                                                                               aTGAGTTTATGCGCGCGCAGTTTAATCCTTTCGATCTTTATCTCGGTACATCTGCCGGGGCGCAGAACCTCTCGGCGTTTATCTGCAATCAGCCCGGTTACGCGCGCAAAGTCATCATGCGCTATACCACAAAACGCGaa                                           <  2:306105/140‑1 (MQ=255)                                                                                tGAGTTTATGCGCGCGCAGTTTAATCCTTTCGATCTTTATCTCGGTACATCTGCCGGGGCGCAGAACCTCTCGGCGTTTATCTGCAATCAGCCCGGTTACGCGCGCAAAGTCATCATGCGCTATACCACAAAACGCGAAt                                          >  2:999525/1‑140 (MQ=255)                                                                                tGAGTTTATGCGCGCGCAGTTTAATCCTTTCGATCTTTATCTCGGTACATCTGCCGGGGCGCAGAACCTCTCGGCGTTTATCTGCAATCAGCCCGGTTACGCGCGCAAAGTCATCATGCGCTATACCACAAAACGCGAAt                                          >  2:1239503/1‑140 (MQ=255)                                                                                     ttATGCGCGCGCAGTTTAATCCTTTCGATCTTTATCTCGGTACATCTGCCGGGGCGCAGAACCTCTCGGCGTTTATCTGCAATCAGCCCGGTTACGCGCGCAAAGTCATCATGCGCTATACCACAAAACGCGAATTTTTc                                     >  1:1202723/1‑140 (MQ=255)                                                                                      tATGCGCGCGCAGTTTAATCCTTTCGATCTTTATCTCGGTACATCTGCCGGGGCGCAGAACCTCTCGGCGTTTATCTGCAATCAGCCCGGTTACGCGCGCAAAGTCATCATGCGCTATACCACAAAACGCGAATTTTTCg                                    >  1:1419651/1‑140 (MQ=255)                                                                                        tGCGCGCGCAGTTTAATCCTTTCGATCTTTATCTCGGTACATCTGCCGGGGCGCAGAACCTCTCGGCGTTTATCTGCAATCAGCCCGGTTACGCGCGCAAAGTCATCATGCGCTATACCACAAAACGCGAATTTTTCGAt                                  >  1:681703/1‑140 (MQ=255)                                                                                           gcgcgcAGTTTAATCCTTTCGATCTTTATCTCGGTACATCTGCCGGGGCGCAGAACCTCTCGGCGTTTATCTGCAATCAGCCCGGTTACGCGCGCAAAGTCATCATGCGCTATACCACAAAACGCGAATTTTTCGATCCa                               <  2:1376947/140‑1 (MQ=255)                                                                                             gcgcAGTTTAATCCTTTCGATCTTTATCTCGGTACATCTGCCGGGGCGCAGAACCTCTCGGCGTTTATCTGCAATCAGCCCGGTTACGCGCGCAAAGTCATCATGCGCTATACCACAAAACGCGAATTTTTCGATCCAtt                             >  2:77852/1‑140 (MQ=255)                                                                                                       aTCCTTTCGATCTTTATCTCGGTACATCTGCCGGGGCGCAGAACCTCTCGGCGTTTATCTGCAATCAGCCCGGTTACGCGCGCAAAGTCATCATGCGCTATACCACAAAACGCGAATTTTTCGATCCATTGCGCTTTGTc                   >  2:737439/1‑140 (MQ=255)                                                                                                            ttCGATCTTTATCTCGGTACATCTGCCGGGGCGCAGAACCTCTCGGCGTTTATCTGCAATCAGCCCGGTTACGCGCGCAAAGTCATCATGCGCTATACCACAAAACGCGAATTTTTCGATCCATTGCGCTTTGTCCGTgg              >  1:365136/1‑140 (MQ=255)                                                                                                             tCGATCTTTATCTCGGTACATCTGCCGGGGCGCAGAACCTCTCGGCGTTTATCTGCAATCAGCCCGGTTACGCGCGCAAAGTCATCATGCGCTATACCACAAAACGCGAATTTTTCGATCCATTGCGCTTTGTCCGTgga             >  1:1411655/1‑140 (MQ=255)                                                                                                             tCGATCTTTATCTCGGTACATCTGCCGGGGCGCAGAACCTCTCGGCGTTTATCTGCAATCAGCCCGGTTACGCGCGCAAAGTCATCATGCGCTATACCACAAAACGCGAATTTTTCGATCCATTGCGCTTTGTCCGTgga             >  1:1291974/1‑140 (MQ=255)                                                                                                             tCGATCTTTATCTCGGTACATCTGCCGGGGCGCAGAACCTCTCGGCGTTTATCTGCAATCAGCCCGGTTACGCGCGCAAAGTCATCATGCGCTATACCACAAAACGCGAATTTTTCGATCCATTGCGCTTTGTCCGTgga             >  2:1295916/1‑140 (MQ=255)                                                                                                                      aTCTCGGTACATCTGCCGGGGCGCAGAACCTCTCGGCGTTTATCTGCAATCAGCCCGGTTACGCGCGCAAAGTCATCATGCGCTATACCACAAAACGCGAATTTTTCGATCCATTGCGCTTTGTCCGTGGAGGAAATCtt    >  1:599784/1‑140 (MQ=255)                                                                                                                       tctcGGTACATCTGCCGGGGCGCAGAACCTCTCGGCGTTTATCTGCAATCAGCCCGGTTACGCGCGCAAAGTCATCATGCGCTATACCACAAAACGCGAATTTTTCGATCCATTGCGCTTTGTCCGTGGAGGAAATCTTa   <  1:1239503/140‑1 (MQ=255)                                                                                                                       tctcGGTACATCTGCCGGGGCGCAGAACCTCTCGGCGTTTATCTGCAATCAGCCCGGTTACGCGCGCAAAGTCATCATGCGCTATACCACAAAACGCGAATTTTTCGATCCATTGCGCTTTGTCCGTGGAGGAAATCTTa   <  1:737439/140‑1 (MQ=255)                                                                                                                        ctcGGTACATCTGCCGGGGCGCAGAACCTCTCGGCGTTTATCTGCAATCAGCCCGGTTACGCGCGCAAAGTCATCATGCGCTATACCACAAAACGCGAATTTTTCGATCCATTGCGCTTTGTCCGTGGAGGAAATCTTAt  <  2:843081/140‑1 (MQ=255)                                                                                                                          cGGTACATCTGCCGGGGCGCAGAACCTCTCGGCGTTTATCTGCAATCAGCCCGGTTACGCGCGCAAAGTCATCATGCGCTATACCACAAAACGCGAATTTTTCGATCCATTGCGCTTTGTCCGTGGAGGaa         <  1:185274/131‑1 (MQ=255)                                                                                                                          cGGTACATCTGCCGGGGCGCAGAACCTCTCGGCGTTTATCTGCAATCAGCCCGGTTACGCGCGCAAAGTCATCATGCGCTATACCACAAAACGCGAATTTTTCGATCCATTGCGCTTTGTCCGTGGAGGaa         >  2:185274/1‑131 (MQ=255)                                                                                                                             |                                                                                                                                       GGCCGTTCCAGCCTGGACGAATAGCTCTGGTGTGCGAAGGCGGCGGACAGCGTGGAATTTTCACGGCTGGCGTGCTGGATGAGTTTATGCGCGCGCAGTTTAATCCTTTCGATCTTTATCTCGGCACATCTGCCGGGGCGCAGAACCTCTCGGCGTTTATCTGCAATCAGCCCGGTTACGCGCGCAAAGTCATCATGCGCTATACCACAAAACGCGAATTTTTCGATCCATTGCGCTTTGTCCGTGGAGGAAATCTTAT  >  NC_000913/4612463‑4612721

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑