Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	616,051	C→T	P632L (CCG→CTG)	entF →	apo‑serine activating enzyme

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	616,051	0	C	T	100.0%	173.0 / NA	49	P632L (CCG→CTG)	entF	apo‑serine activating enzyme
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (24/25);  total (24/25)

GCGCCGCTGCAACTTTCACAACCGCATCACACGGCTTATATCATCTTTACCTCTGGCTCCACCGGCAGGCCGAAAGGGGTAATGGTCGGGCAGACGGCTATCGTCAACCGCCTGCTTTGGATGCAAAATCATTATCCGCTTACAGGCGAAGATGTCGTTGCCCAAAAAACGCCGTGCAGTTTTGATGTCTCGGTGTGGGAGTTTTTCTGGCCGTTTATCGCAGGGGCAAAACTGGTGATGGCTGAACCGGAAGCGCACCGCGACCCGCTCG  >  NC_000913/615915‑616185                                                                                                                                         |                                                                                                                                       gcgcCGCTGCAACTTTCACAACCGCATCACACGGCTTATATCATCTTTACCTCTGGCTCCACCGGCAGGCCGAAAGGGGTAATGGTCGTGCAGACGGCTATCGTCAACCGCCTGCTTTGGATGCAAAATCATTATCTGCt                                                                                                                                     <  2:992218/140‑1 (MQ=255)   gccgcTGCAATTTTCACACCCGCATCACACGGTTTATATCATTTTTCCCTCTGGCTCCCCCGGCAGGCCGAAAGGGGTAATGGTGGGGCAGACGGTTATCGTCAACCGCCTGCTTTGGATGCAAAATCATTATCTGCTTa                                                                                                                                   <  2:708710/140‑1 (MQ=255)     cgcTGCAACTTTCACAACCGCATCACACGGCTTATATCATCTTTACCTCTGGCTCCACCGGCAGGCCGAAAGGGGTAATGGTCGGGCAGACGGCTATCGTCAACCGCCTGCTTTGGATGCAAAATCATTATCTGCTTACa                                                                                                                                 <  1:235313/140‑1 (MQ=255)       cTGCAACTTTCACAACCGCATCACACGGCTTATATCATCTTTACCTCTGGCTCCACCGGCAGGCCGAAAGGGGTAATGGTCGGGCAGACGGCTATCGTCAACCGCCTGCTTTGGATGCAAAATCATTATCTGCTTACAgg                                                                                                                               >  2:1004721/1‑140 (MQ=255)                       cGCATCACACGGCTTATATCATCTTTACCTCTGGCTCCACCGGCAGGCCGAAAGGGGTAATGGTCGGGCAGACGGCTATCGTCAACCGCCTGCTTTGGATGCAAAATCATTATCTGCTTACAGGCGAAGATGTCGTTGcc                                                                                                               >  2:144648/1‑140 (MQ=255)                         cATCACACGGCTTATATCATCTTTACCTCTGGCTCCACCGGCAGGCCGAAAGGGGTAATGGTCGGGCAGACGGCTATCGTCAACCGCCTGCTTTGGATGCAAAATCATTATCTGCTTACAGGCGAAGa                                                                                                                         <  2:291262/128‑1 (MQ=255)                         cATCACACGGCTTATATCATCTTTACCTCTGGCTCCACCGGCAGGCCGAAAGGGGTAATGGTCGGGCAGACGGCTATCGTCAACCGCCTGCTTTGGATGCAAAATCATTATCTGCTTACAGGCGAAGa                                                                                                                         >  1:291262/1‑128 (MQ=255)                          aTCACACGGCTTATATCATCTTTACCTCTGGCTCCACCGGCAGGCCGAAAGGGGTAATGGTCGGGCAGACGGCTATCGTCAACCGCCTGCTTTGGATGCAAAATCATTATCTGCTTACAGGCGAAGATGTCGTTGCCCaa                                                                                                            >  2:1068092/1‑140 (MQ=255)                                cGGCTTATATCATCTTTACCTCTGGCTCCACCGGCAGGCCGAAAGGGGTAATGGTCGGGCAGACGGCTATCGTCAACCGCCTGCTTTGGATGCAAAATCATTATCTGCTTACAGGCGAAGATGTCGTTGCCCAAAAAACg                                                                                                      <  2:613605/140‑1 (MQ=255)                                   cTTATATCATCTTTACCTCTGGCTCCACCGGCAGGCCGAAAGGGGTAATGGTCGGGCAGACGGCTATCGTCAACCGCCTGCTTTGGATGCAAAATCATTATCTGCTTACAGGCGAAGATGTCGTTGCCCAAAAAACGCCg                                                                                                   >  1:630987/1‑140 (MQ=255)                                            tcTTTACCTCTGGCTCCACCGGCAGGCCGAAAGGGGTAATGGTCGGGCAGACGGCTATCGTCAACCGCCTGCTTTGGATGCAAAATCATTATCTGCTTACAGGCGAAGATGTCGTTGCCCAAAAAACGCCGTGCAGtttt                                                                                          >  1:333925/1‑140 (MQ=255)                                             cTTTACCTCTGGCTCCACCGGCAGGCCGAAAGGGGTAATGGTCGGGCAGACGGCTATCGTCAACCGCCTGCTTTGGATGCAAAATCATTATCTGCTTACAGGCGAAGATGTCGTTGCCCAAAAAACGCCGTg                                                                                                 <  2:461115/132‑1 (MQ=255)                                             cTTTACCTCTGGCTCCACCGGCAGGCCGAAAGGGGTAATGGTCGGGCAGACGGCTATCGTCAACCGCCTGCTTTGGATGCAAAATCATTATCTGCTTACAGGCGAAGATGTCGTTGCCCAAAAAACGCCGTg                                                                                                 >  1:461115/1‑132 (MQ=255)                                             cTTTACCTCTGGCTCCACCGGCAGGCCGAAAGGGGTAATGGTCGGGCAGACGGCTATCGTCAACCGCCTGCTTTGGATGCAAAATCATTATCTGCTTACAGGCGAAGATGTCGTTGCCCAAAAAACGCCGTGCAGTTTTg                                                                                         >  2:1164931/1‑140 (MQ=255)                                             cTTTACCTCTGGCTCCACCGGCAGGCCGAAAGGGGTAATGGTCGGGCAGACGGCTATCGTCAACCGCCTGCTTTGGATGCAAAATCATTATCTGCTTACAGGCGAAGATGTCGTTGCCCAAAAAACGCCGTGCAGTTTTg                                                                                         >  2:346908/1‑140 (MQ=255)                                             cTTTACCTCTGGCTCCACCGGCAGGCCGAAAGGGGTAATGGTCGGGCAGACGGCTATCGTCAACCGCCTGCTTTGGATGCAAAATCATTATCTGCTTACAGGCGAAGATGTCGTTGCCCAAAAAACGCCGTGCAGTTTTg                                                                                         >  2:172552/1‑140 (MQ=255)                                               ttACCTCTGGCTCCACCGGCAGGCCGAAAGGGGTAATGGTCGGGCAGACGGCTATCGTCAACCGCCTGCTTTGGATGCAAAATCATTATCTGCTTACAGGCGAAGATGTCGTTGCCCAAAAAACGCCGTGCAGTTTTGAt                                                                                       <  2:333925/140‑1 (MQ=255)                                                 aCCTCTGGCTCCACCGGCAGGCCGAAAGGGGTAATGGTCGGGCAGACGGCTATCGTCAACCGCCTGCTTTGGATGCAAAATCATTATCTGCTTACAGGCGAAGATGTCGTTGCCCAAAAAACGCCGTGCAGTTTTGAt                                                                                       >  1:1199383/1‑138 (MQ=255)                                                 aCCTCTGGCTCCACCGGCAGGCCGAAAGGGGTAATGGTCGGGCAGACGGCTATCGTCAACCGCCTGCTTTGGATGCAAAATCATTATCTGCTTACAGGCGAAGATGTCGTTGCCCAAAAAACGCCGTGCAGTTTTGAt                                                                                       <  2:1199383/138‑1 (MQ=255)                                                 aCCTCTGGCTCCACCGGCAGGCCGAAAGGGGTAATGGTCGGGCAGACGGCTATCGTCAACCGCCTGCTTTGGATGCAAAATCATTATCTGCTTACAGGCGAAGATGTCGTTGCCCAAAAAACGCCGTGCAGTTTTGATGt                                                                                     >  2:397452/1‑140 (MQ=255)                                                 aCCTCTGGCTCCACCGGCAGGCCGAAAGGGGTAATGGTCGGGCAGACGGCTATCGTCAACCGCCTGCTTTGGATGCAAAATCATTATCTGCTTACAGGCGAAGATGTCGTTGCCCAAAAAACGCCGTGCAGTTTTGATGt                                                                                     >  2:520431/1‑140 (MQ=255)                                                 aCCTCTGGCTCCACCGGCAGGCCGAAAGGGGTAATGGTCGGGCAGACGGCTATCGTCAACCGCCTGCTTTGGATGCAAAATCATTATCTGCTTACAGGCGAAGATGTCGTTGCCCAAAAAACGCCGTGCAGTTTTGATGt                                                                                     >  1:230595/1‑140 (MQ=255)                                                  ccTCTGGCTCCACCGGCAGGCCGAAAGGGGTAATGGTCGGGCAGACGGCTATCGTCAACCGCCTGCTTTGGATGCAAAATCATTATCTGCTTACAGGCGAAGATGTCGTTGCCCAAAAAACGCCGTGCAGTTTTGATGtc                                                                                    >  2:90354/1‑140 (MQ=255)                                                   ctctGGCTCCACCGGCAGGCCGAAAGGGGTAATGGTCGGGCAGACGGCTATCGTCAACCGCCTGCTTTGGATGCAAAATCATTATCTGCTTACAGGCGAAGATGTCGTTGCCCAAAAAACGCCGTGCAGTTTTGATGtct                                                                                   <  1:346908/140‑1 (MQ=255)                                                     ctGGCTCCACCGGCAGGCCGAAAGGGGTAATGGTCGGGCAGACGGCTATCGTCAACCGCCTGCTTTGGATGCAAAATCATTATCTGCTTACAGGCGAAGATGTCGTTGCCCAAAAAACGCCGTGCAGTTTTGATGTCTCg                                                                                 <  1:1164931/140‑1 (MQ=255)                                                       ggCTCCACCGGCAGGCCGAAAGGGGTAATGGTCGGGCAGACGGCTATCGTCAACCGCCTGCTTTGGATGCAAAATCATTATCTGCTTGCAGGCGAAGATGTCGTTGCCCAAAAAACGCCGTGCAGTTTTGATGTCTCGgt                                                                               >  1:859449/1‑140 (MQ=255)                                                                   aGGCCGAAAGGGGTAATGGTCGGGCAGACGGCTATCGTCAACCGCCTGCTTTGGATGCAAAATCATTATCTGCTTACAGGCGAAGATGTCGTTGCCCAAAAAACGCCGTGCAGTTTTGATGTCTCGGTGTGGGAGttttt                                                                   >  2:74189/1‑140 (MQ=255)                                                                      ccGAAAGGGGTAATGGTCGGGCAGACGGCTATCGTCAACCGCCTGCTTTGGATGCAAAATCATTATCTGCTTACAGGCGAAGATGTCGTTGCCCAAAAAACGCCGTGCAGTTTTGATGTCTCGGTGTGGGGGTTTTTCTg                                                                >  1:1414249/1‑140 (MQ=255)                                                                      ccGAAAGGGGTAATGGTCGGGCAGACGGCTATCGTCAACCGCCTGCTTTGGATGCAAAATCATTATCTGCTTACAGGCGAAGATGTCGTTGCCCAAAAAACGCCGTGCAGTTTTGATGTCTCGGTGTGGGAGTTTTTCTg                                                                >  1:230892/1‑140 (MQ=255)                                                                       cGAAAGGGGTAATGGTCGGGCAGACGGCTATCGTCAACCGCCTGCTTTGGATGCAAAATCATTATCTGCTTACAGGCGAAGATGTCGTTGCCCAAAAAACGCCGTGCAGTTTTGATGTCTCGGTGTGGGAGTTTTTCTgg                                                               >  1:977950/1‑140 (MQ=255)                                                                       cGAAAGGGGTAATGGTCGGGCAGACGGCTATCGTCAACCGCCTGCTTTGGATGCAAAATCATTATCTGCTTACAGGCGAAGATGTCGTTGCCCAAAAAACGCCGTGCAGTTTTGATGTCTCGGTGTGGGAGTTTTTCTgg                                                               <  1:90354/140‑1 (MQ=255)                                                                       cGAAAGGGGTAATGGTCGGGCAGACGGCTATCGTCAACCGCCTGCTTTGGATGCAAAATCATTATCTGCTTACAGGCGAAGATGTCGTTGCCCAAAAAACGCCGTGCAGTTTTGATGTCTCGGTGTGGGAGTTTTTCTgg                                                               <  1:513023/140‑1 (MQ=255)                                                                       cGAAAGGGGTAAGGGTGGGGCAGACGGCTATCGTCAACCGCCTGCTTTGGATGCAAAATCATTATCTGCTTACAGGCGAAGATGTCGTTGCCCAAAAAACGCCGTGCAGTTTTGATGTCTCGGTGTGGGAGTTTTTCTgg                                                               <  1:177700/140‑1 (MQ=255)                                                                               gTAATGGTCGGGCAGACGGCTATCGTCAACCGCCTGCTTTGGATGCAAAATCATTATCTGCTTACAGGCGAAGATGTCGTTGCCCAAAAAACGCCGTGCAGTTTTGATGTCTCGGTGTGGGAGTTTTTCTGGCCGTTTAt                                                       >  1:173868/1‑140 (MQ=255)                                                                                 aaTGGTCGGGCAGACGGCTATCGTCAACCGCCTGCTTTGGATGCAAAATCATTATCTGCTTACAGGCGAAGATGTCGTTGCCCCAAAAACGCCGTGCAGTTTTGATGTCTCGGTGTGGGAG‑TTTTCTGGCCGTTTATCGc                                                    <  2:668294/140‑1 (MQ=255)                                                                                       cGGGCAGACGGCTATCGTCAACCGCCTGCTTTGGATGCAAAATCATTATCTGCTTACAGGCGAAGATGTCGTTGCCCAAAAAACGCCGTGCAGTTTTGATGTCTCGGTGTGGGAGTTTTTCTGGCCGTTTATCGCAgggg                                               <  2:230892/140‑1 (MQ=255)                                                                                          gCAGACGGCTATCGTCAACCGCCTGCTTTGGATGCAAAATCATTATCTGCTTACAGGCGAAGATGTCGTTGCCCAAAAAACGCCGTGCAGTTTTGATGTCTCGGTGTGGGAGTTTTTCTGGCCGTTTATCGCAGGGGCaa                                            <  2:230595/140‑1 (MQ=255)                                                                                            aGACGGCTATCGTCAACCGCCTGCTTTGGATGCAAAATCATTATCTGCTTACAGGCGAAGATGTCGTTGCCCAAAAAACGCCGTGCAGTTTTGATGTCTCGGTGTGGGAGTTTTTCTGGCCGTTTATCGCAGGGGCaaaa                                          <  2:728149/140‑1 (MQ=255)                                                                                                  cTATCGTCAACCGCCTGCTTTGGATGCAAAATCATCATCTGCTTACAGGCGAAGATGTCGTTGCCCAAAAAACGCCGTGCAGTTTTGATGTCTCGGTGTGGGAGTTTTTCTGGCCGTTTATCGCAGGGGCAAAACTGGTg                                    <  2:977950/140‑1 (MQ=255)                                                                                                       gTCAACCGCCTGCTTTGGATGCAAAATCATTATCTGCTTACAGGCGAAGATGTCGTTGCCCAAAAAACGCCGTGCAGTTTTGATGTCTCGGTGTGGGAGTTTTTCTGGCCGTTTATCGCAGGGGCAAAACTGGTGATGGc                               <  2:630987/140‑1 (MQ=255)                                                                                                        tCAACCGCCTGCTTTGGATGCAAAATCATTATCTGCTTACAGGCGAAGATGTCGTTGCCCAAAAAACGCCGTGCAGTTTTGATGTCTCGGTGTGGGAGTTTTTCTGGCCGTTTATCGCAGGGGCAAAACTGGTGATGGCt                              >  2:400510/1‑140 (MQ=255)                                                                                                        tCAACCGCCTGCTTTGGATGCAAAATCATTATCTGCTTACAGGCGAAGATGTCGTTGCCCAAAAAACGCCGTGCAGTTTTGATGTCTCGGTGTGGAAGTTTTTCTGGCCGTTTATCGCAGGGGCAAAACTGGTGATGGCt                              <  1:1004721/140‑1 (MQ=255)                                                                                                               ccTGCTTTGGATGCAAAATCATTATCTGCTTACAGGCGAAGATGTCGTTGCCCAAAAAACGCCGTGCAGTTTTGATGTCTCGGTGTGGGAGTTTTTCTGGCCGTTTATCGCAGGGGCAAAACTGGTGATGGCTGAACCgg                       <  1:520431/140‑1 (MQ=255)                                                                                                               ccTGCTTTGGATGCAAAATCATTATCTGCTTACAGGCGAAGATGTCGTTGCCCAAAAAACGCCGTGCAGTTTTGATGTCTCGGTGTGGGAGTTTTTCTGGCCGTTTATCGCAGGGGCAAAACTGGTGATGGCTGAACCgg                       <  1:397452/140‑1 (MQ=255)                                                                                                                    tttGGATGCAAAATCATTATCTGCTTACAGGCGAAGATGTCGTTGCCCAAAAAACGCCGTGCAGTTTTGATGTCTCGGTGTGGGGGTTTTTCTGGCCGTTTATCGCAGGGGCAAAACTGGTGATGGCTGAACCGGAAgcg                  <  2:1414249/140‑1 (MQ=255)                                                                                                                       ggATGCAAAATCATTATCTGCTTGCAGGCGAAGATGTCGTTGCCCAAAAAACGCCGTGCAGTTTTGATGTCTCGGTGTGGGAGTTTTTCTGGCCGTTTATCGCAGGGGCAAAACTGGTGATGGCTGAACCGGAAGCGCAc               <  2:859449/140‑1 (MQ=255)                                                                                                                             aaaaTCATTATCTGCTTACAGGCGAAGATGTCGTTGCCCAAAAAACGCCGTGTAGTTTTGATGTCTCGGTGTGGGAGTTTTTCTGGCCGTTTATCGCAGGGGCAAAACTGGTGATGGCTGAACCGGAAGCGCACCGCGAc         >  1:627462/1‑140 (MQ=255)                                                                                                                             aaaaTCATTATCTGCTTACAGGCGAAGATGTCGTTGCCCAAAAAACGCCGTGCAGTTTTGATGTCTCGGTGTGGGAGTTTTTCTGGCCGTTTATCGCAGGGGCAAAACTGGTGATGGCTGAACCGGAAGCGCACCGCGAc         >  2:1379724/1‑140 (MQ=255)                                                                                                                                    ttATCTGCTTACAGGCGAAGATGTCGTTGCCCAAAAAACGCCGTGCAGTTTTGATGTCTCGGTGTGGGAGTTTTTCTGGCCGTTTATCGCAGGGGCAAAACTGGTGATGGCTGAACCGGAAGCGCACCGCGACCcgctcg  <  1:144648/140‑1 (MQ=255)                                                                                                                                         |                                                                                                                                       GCGCCGCTGCAACTTTCACAACCGCATCACACGGCTTATATCATCTTTACCTCTGGCTCCACCGGCAGGCCGAAAGGGGTAATGGTCGGGCAGACGGCTATCGTCAACCGCCTGCTTTGGATGCAAAATCATTATCCGCTTACAGGCGAAGATGTCGTTGCCCAAAAAACGCCGTGCAGTTTTGATGTCTCGGTGTGGGAGTTTTTCTGGCCGTTTATCGCAGGGGCAAAACTGGTGATGGCTGAACCGGAAGCGCACCGCGACCCGCTCG  >  NC_000913/615915‑616185

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑