Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	619,112	G→T	A287S (GCG→TCG)	fepE →	polysaccharide co‑polymerase family protein FepE

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	619,112	0	G	T	100.0%	302.8 / NA	84	A287S (GCG→TCG)	fepE	polysaccharide co‑polymerase family protein FepE
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (42/42);  total (42/42)

TATTCACTCGACATTGCCAACGCGGCAGGAATTAAAAAGCCCGTTTACAGTAATGGTCAGGCCGTTAAAGATGACCCCGATTTTTCTATTTCTCTCGGTGCAGACGGTATTGAACGCAAACTGGAAATAGAAAAAGCGGTCACTGACGTTGCGGAACTGAACGGTGAATTACGTAATCGGCAGTATCTTGTCGAGCAATTAACAAAAGCACATGTCAACGATGTGAATTTTACGCCGTTTAAATATCAGTTAAGCCCGTCATTGCCAGTGAA  >  NC_000913/618977‑619248                                                                                                                                        |                                                                                                                                         tattCACTCGACATTGCCAACGCGGCAGGAATTAAAAAGCCCGTTTACAGTAATGGTCAGGCCGTTAAAGATGACCCCGATTTTTCTATTTCTCTCGGTGCAGACGGTATTGAACGCAAACTGGAAATAGAAAAATCGGt                                                                                                                                      <  2:566864/140‑1 (MQ=255)     cACTCGACATTGCCAACGCGGCAGGAATTAAAAAGCCCGTTTACAGTAATGGTCAGGCCGTTAAAGATGACCCCGATTTTTCTATTTCTCTCGGTGCAGACGGTATTGAACGCAAACTGGAAATAGAAAAATCGGTCACt                                                                                                                                  >  2:1344237/1‑140 (MQ=255)       cTCGACATTGCCAGCGCGGCAGGAATTAAAAAGCCCGTTTACAGTAATGGTCAGGCCGTTAAAGATGACCCCGATTTTTCTATTTCTCTCGGTGCAGACGGTATTGAACGCAAACTGGAAATAGAAAAATCGGTCACTGa                                                                                                                                <  2:938628/140‑1 (MQ=255)               tGCCAACGCGGCAGGAATTAAAAAGCCCGTTTACAGTAATGGTCAGGCCGTTAAAGATGACCCCGATTTTTCTATTTCTCTCGGTGCAGACGGTATTGAACGCAAACTGGAAATAGAAAAATCGGTCACTGACGTTGCgg                                                                                                                        >  2:256063/1‑140 (MQ=255)                gCCAACGCGGCAGGAATTAAAAAGCCCGTTTACAGTAATGGTCAGGCCGTTAAAGATGACCCCGATTTTTCTATTTCTCTCGGTGCAGACGGTATTGAACGCAAACTGGAAATAGAAAAATCGGTCACTGACGTTGCGGa                                                                                                                       >  1:1378234/1‑140 (MQ=255)                 ccAACGCGGCAGGAATTAAAAAGCCCGTTTACAGTAATGGTCAGGCCGTTAAAGATGACCCCGATTTTTCTATTTCTCTCGGTGCAGACGGTATTGAACGCAAACTGGAAATAGAAAAATCGGTCACTGACGTTGCGGaa                                                                                                                      >  2:1342581/1‑140 (MQ=255)                   aaCGCGGCAGGAATTAAAAAGCCCGTTTACAGTAATGGTCAGGCCGTTAAAGATGACCCCGATTTTTCTATTTCTCTCGGTGCAGACGGTATTGAACGCAAACTGGAAATAGAAAAATCGGTCACTGACGTTGCGGAACt                                                                                                                    <  1:1300370/140‑1 (MQ=255)                    aCGCGGCAGGAATTAAAAAGCCCGTTTACAGTAATGGTCAGGCCGTTAAAGATGACCCCGATTTTTCTATTTCTCTCGGTGCAGACGGTATTGAACGCAAACTGGAAATAGAAAAATCGGTCACTGACGTTGCGGAACTg                                                                                                                   <  2:1298487/140‑1 (MQ=255)                    aCGCGGCAGGAATTAAAAAGCCCGTTTACAGTAATGGTCAGGCCGTTAAAGATGACCCCGATTTTTCTATTTCTCTCGGTGCAGACGGTATTGAACGCAAACTGGAAATAGAAAAATCGGTCACTGACGTTGCGGAACTg                                                                                                                   >  1:1298487/1‑140 (MQ=255)                      gcgGCAGGAATTAAAAAGCCCGTTTACAGTAATGGTCAGGCCGTTAAAGATGACCCCGATTTTTCTATTTCTCTCGGTGCAGACGGTATTGAACGCAAACTGGAAATAGAAAAATCGGTCACTGACGTTGCGGAACTGaa                                                                                                                 >  2:1229005/1‑140 (MQ=255)                      gcgGCAGGAATTAAAAAGCCCGTTTACAGTAATGGTCAGGCCGTTAAAGATGACCCCGATTTTTCTATTTCTCTCGGTGCAGACGGTATTGAACGCAAACTGGAAATAGAAAAATCGGTCACTGACGTTGCGGAACTGaa                                                                                                                 <  2:1049215/140‑1 (MQ=255)                      gcgGCAGGAATTAAAAAGCCCGTTTACAGTAATGGTCAGGCCGTTAAAGATGACCCCGATTTTTCTATTTCTCTCGGTGCAGACGGTATTGAACGCAAACTGGAAATAGAAAAATCGGTCACTGACGTTGCGGAACTGa                                                                                                                  >  2:816213/1‑139 (MQ=255)                      gcgGCAGGAATTAAAAAGCCCGTTTACAGTAATGGTCAGGCCGTTAAAGATGACCCCGATTTTTCTATTTCTCTCGGTGCAGACGGTATTGAACGCAAACTGGAAATAGAAAAATCGGTCACTGACGTTGCGGAACTGa                                                                                                                  <  1:816213/139‑1 (MQ=255)                         gCAGGAATTAAAAAGCCCGTTTACAGTAATGGTCAGGCCGTTAAAGATGACCCCGATTTTTCTATTTCTCTCGGTGCAGACGGTATTGAACGCAAACTGGAAATAGAAAAATCGGTCACTGACGTTGCGGAACTGAACgg                                                                                                              >  1:1236056/1‑140 (MQ=255)                         gCAGGAATTAAAAAGCCCGTTTACAGTAATGGTCAGGCCGTTAAAGATGACCCCGATTTTTCTATTTCTCTCGGTGCAGACGGTATTGAACGCAAACTGGAAATAGAAAAATCGGTCACTGACGTTGCGGAACTGAACgg                                                                                                              >  2:455283/1‑140 (MQ=255)                         gCAGGAATTAAAAAGCCCGTTTACAGTAATGGTCAGGCCGTTAAAGATGACCCCGATTTTTCTATTTCTCTCGGTGCAGACGGTATTGAACGCAAACTGGAAATAGAAAAATCGGTCACTGACGTTGCGGAACTGAACg                                                                                                               <  2:337594/139‑1 (MQ=255)                         gCAGGAATTAAAAAGCCCGTTTACAGTAATGGTCAGGCCGTTAAAGATGACCCCGATTTTTCTATTTCTCTCGGTGCAGACGGTATTGAACGCAAACTGGAAATAGAAAAATCGGTCACTGACGTTGCGGAACTGAACg                                                                                                               >  1:337594/1‑139 (MQ=255)                          cAGGAATTAAAAAGCCCGTTTACAGTAATGGTCAGGCCGTTAAAGATGACCCCGATTTTTCTATTTCTCTCGGTGCAGACGGTATTGAACGCAAACTGGAAATAGAAAAATCGGTCACTGACGTTGCGGAACTGAACGGt                                                                                                             >  1:374398/1‑140 (MQ=255)                                    aaaGCCCGTTTACAGTAATGGTCAGGCCGTTAAAGATGACCCCGATTTTTCTATTTCTCTCGGTGCAGACGGTATTGAACGCAAACTGGAAATAGAAAAATCGGTCACTGACGTTGCGGAACTGAACGGTGAATTACGTa                                                                                                   <  1:455283/140‑1 (MQ=255)                                    aaaGCCCGTTTACAGTAATGGTCAGGCCGTTAAAGATGACCCCGATTTTTCTATTTCTCTCGGTGCAGACGGTATTGAACGCAAACTGGAAATAGAAAAATCGGTCACTGACGTTGCGGAACTGAACGGTGAATTACGTa                                                                                                   <  2:688597/140‑1 (MQ=255)                                     aaGCCCGTTTACAGTAATGGTCAGGCCGTTAAAGATGACCCCGATTTTTCTATTTCTCTCGGTGCAGACGGTATTGAACGCAAACTGGAAATAGAAAAATCGGTCACTGACGTTGCGGAACTGAACGGTGAATTACGTaa                                                                                                  <  1:1342581/140‑1 (MQ=255)                                      aGCCCGTTTACAGTAATGGTCAGGCCGTTAAAGATGACCCCGATTTTTCTATTTCTCTCGGTGCAGACGGTATTGAACGCAAACTGGAAATAGAAAAATCGGTCACTGACGTTGCGGAACTGAACGGTGAATTACGTAAt                                                                                                 <  2:568968/140‑1 (MQ=255)                                         ccGTTTACAGTAATGGTCAGGCCGTTAAAGATGACCCCGATTTTTCTATTTCTCTCGGTGCAGACGGTATTGAACGCAAACTGGAAATAGAAAAATCGGTCACTGACGTTGCGGAACTGAACGGTGAATTACGTAATCgg                                                                                              <  2:772424/140‑1 (MQ=255)                                         ccGTTTACAGTAATGGTCAGGCCGTTAAAGATGACCCCGATTTTTCTATTTCTCTCGGTGCAGACGGTATTGAACGCAAACTGGAAATAGAAAAATCGGTCACTGACGTTGCGGAACTGAACGGTGAATTACGTAATCgg                                                                                              >  2:1085055/1‑140 (MQ=255)                                         ccGTTTACAGTAATGGTCAGGCCGTTAAAGATGACCCCGATTTTTCTATTTCTCTCGGTGCAGACGGTATTGAACGCAAACTGGAAATAGAAAAATCGGTCACTGACGTTGCGGAACTGAACGGTGAATTACGTAATCgg                                                                                              <  1:207440/140‑1 (MQ=255)                                           gTTTACAGTAATGGTCAGGCCGTTAAAGATGACCCCGATTTTTCTATTTCTCTCGGTGCAGACGGTATTGAACGCAAACTGGAAATAGAAAAATCGGTCACTGACGTTGCGGAACTGAACGGTGAATTACGTAATCGGCa                                                                                            <  1:775800/140‑1 (MQ=255)                                           gTTTACAGTAATGGTCAGGCCGTTAAAGATGACCCCGATTTTTCTATTTCTCTCGGTGCAGACGGTATTGAACGCAAACTGGAAATAGAAAAATCGGTCACTGACGTTGCGGAACTGAACGGTGAATTACGTAATCGGCa                                                                                            <  2:866463/140‑1 (MQ=255)                                                      tGGTCAGGCCGTTAAAGATGACCCCGATTTTTCTATTTCTCTCGGTGCAGACGGTATTGAACGCAAACTGGAAATAGAAAAATCGGTCACTGACGTTGCGGAACTGAACGGTGAATTACGTAATCGGCAGTATCTTGTCg                                                                                 >  1:1323510/1‑140 (MQ=255)                                                        gTCAGGCCGTTAAAGATGACCCCGATTTTTCTATTTCTCTCGGTGCAGACGGTATTGAACGCAAACTGGAAATAGAAAAATCGGTCACTGACGTTGCGGAACTGAACGGTGAATTACGTAATCGGCAGTATCTTGTCGAg                                                                               <  1:200929/140‑1 (MQ=255)                                                        gTCAGGCCGTTAAAGATGACCCCGATTTTTCTATTTCTCTCGGTGCAGACGGTATTGAACGCAAACTGGAAATAGAAAAATCGGTCACTGACGTTGCGGAACTGAACGGTGAATTACGTAATCGGCAGTATCTTGTCGAg                                                                               >  2:200929/1‑140 (MQ=255)                                                        gTCAGGCCGTTAAAGATGACCCCGATTTTTCTATTTCTCTCGGTGCAGACGGTATTGAACGCAAACTGGAAATAGAAAAATCGGTCACTGACGTTGCGGAACTGAACGGTGAATTACGTAATCGGCAGTATCTTGTCGAg                                                                               >  2:746415/1‑140 (MQ=255)                                                           aGGCCGTTAAAGATGACCCCGATTTTTCTATTTCTCTCGGTGCAGACGGTATTGAACGCAAACTGGAAATAGAAAAATCGGTCACTGACGTTGCGGAACTGAACGGTGAATTACGTAATCGGCAGTATCTTGTCGAGCaa                                                                            >  1:1159818/1‑140 (MQ=255)                                                             gCCGTTAAAGATGACCCCGATTTTTCTATTTCTCTCGGTGCAGACGGTATTGAACGCAAACTGGAAATAGAAAAATCGGTCACTGACGTTGCGGAACTGAACGGTGAATTACGTAATCGGCAGTATCTTGTCGAGCAAtt                                                                          >  1:1206801/1‑140 (MQ=255)                                                               cGTTAAAGATGACCCCGATTTTTCTATTTCTCTCGGTGCAGACGGTATTGAACGCAAACTGGAAATAGAAAAATCGGTCACTGACGTTGCGGAACTGAACGGTGAATTACGTAATCGGCAGTATCTTGTCGAGCAATTaa                                                                        >  1:100539/1‑140 (MQ=255)                                                                 ttAAAGATGACCCCGATTTTTCTATTTCTCTCGGTGCAGACGGTATTGAACGCAAACTGGAAATAGAAAAATCGGTCACTGACGTTGCGGAACTGAACGGTGAATTACGTAATCGGCAGTATCTTGTCGAGCAATTAACa                                                                      >  2:1416023/1‑140 (MQ=255)                                                                 ttAAAGATGACCCCGATTTTTCTATTTCTCTCGGTGCAGACGGTATTGAACGCAAACTGGAAATAGAAAAATCGGTCACTGACGTTGCGGAACTGAACGGTGAATTACGTAATCGGCAGTATCTTGTCGAGCAATTAACa                                                                      >  2:1372966/1‑140 (MQ=255)                                                                  tAAAGATGACCCCGATTTTTCTATTTCTCTCGGTGCAGACGGTATTGAACGCAAACTGGAAATAGAAAAATCGGTCACTGACGTTGCGGAACTGAACGGTGAATTACGTAATCGGCAGTATCTTGTCGAGCAATTAACaa                                                                     >  1:402811/1‑140 (MQ=255)                                                                     aGATGACCCCGATTTTTCTATTTCTCTCGGTGCAGACGGTATTGAACGCAAACTGGAAATAGAAAAATCGGTCACTGACGTTGCGGAACTGAACGGTGAATTACGTAATCGGCAGTATCTTGTCGAGCAATTAACAAAAg                                                                  <  1:102700/140‑1 (MQ=255)                                                                     aGATGACCCCGATTTTTCTATTTCTCTCGGTGCAGACGGTATTGAACGCAAACTGGAAATAGAAAAATCGGTCACTGACGTTGCGGAACTGAACGGTGAATTACGTAATCGGCAGTATCTTGTCGAGCAATTAACAAAAg                                                                  <  2:402811/140‑1 (MQ=255)                                                                     aGATGACCCCGATTTTTCTATTTCTCTCGGTGCAGACGGTATTGAACGCAAACTGGAAATAGAAAAATCGGTCACTGACGTTGCGGAACTGAACGGTGAATTACGTAATCGGCAGTATCTTGTCGAGCAATTAACAAAAg                                                                  <  1:1125046/140‑1 (MQ=255)                                                                     aGATGACCCCGATTTTTCTATTTCTCTCGGTGCAGACGGTATTGAACGCAAACTGGAAATAGAAAAATCGGTCACTGACGTTGCGGAACTGAACGGTGAATTACGTAATCGGCAGTATCTTGTCGAGCAATTAACAAAAg                                                                  <  1:486906/140‑1 (MQ=255)                                                                     aGATGACCCCGATTTTTCTATTTCTCTCGGTGCAGACGGTATTGAACGCAAACTGGAAATAGAAAAATCGGTCACTGACGTTGCGGAACTGAACGGTGAATTACGTAATCGGCAGTATCTTGTCGAGCAATTAACAAAAg                                                                  <  2:411698/140‑1 (MQ=255)                                                                       aTGACCCCGATTTTTCTATTTCTCTCGGTGCAGACGGTATTGAACGCAAACTGGAAATAGAAAAATCGGTCACTGACGTTGCGGAACTGAACGGTGAATTACGTAATCGGCAGTATCTTGTCGAg                                                                               >  1:180687/1‑125 (MQ=255)                                                                       aTGACCCCGATTTTTCTATTTCTCTCGGTGCAGACGGTATTGAACGCAAACTGGAAATAGAAAAATCGGTCACTGACGTTGCGGAACTGAACGGTGAATTACGTAATCGGCAGTATCTTGTCGAg                                                                               <  2:180687/125‑1 (MQ=255)                                                                       aTGACCCCGATTTTTCTATTTCTCTCGGTGCAGACGGTATTGAACGCAAACTGGAAATAGAAAAATCGGTCACTGACGTTGCGGAACTGAACGGTGAATTACGTAATCGGCAGTATCTTGTCGAGCAATTAACAAAAGca                                                                <  1:746415/140‑1 (MQ=255)                                                                       aTGACCCCGATTTTTCTATTTCTCTCGGTGCAGACGGTATTGAACGCAAACTGGAAATAGAAAAATCGGTCACTGACGTTGCGGAACTGAACGGTGAATTACGTAATCGGCAGTATCTTGTCGAGCAATTAACAAAAGca                                                                <  2:1323510/140‑1 (MQ=255)                                                                       aTGACCCCGATTTTTCTATTTCTCTCGGTGCAGACGGTATTGAACGCAAACTGGAAATAGAAAAATCGGTCACTGACGTTGCGGAACTGAACGGTGAATTACGTAATCGGCAGTATCTTGTCGAGCAATTAACAAAAGca                                                                <  1:1372966/140‑1 (MQ=255)                                                                       aTGACCCCGATTTTTCTATTTCTCTCGGTGCAGACGGTATTGAACGCAAACTGGAAATAGAAAAATCGGTCACTGACGTTGCGGAACTGAACGGTGAATTACGTAATCGGCAGTATCTTGTCGAGCAATTAACAAAAGca                                                                <  1:1416023/140‑1 (MQ=255)                                                                       aTGACCCCGATTTTTCTATTTCTCTCGGTGCAGACGGTATTGAACGCAAACTGGAAATAGAAAAATCGGTCACTGACGTTGCGGAACTGAACGGTGAATTACGTAATCGGCAGTATCTTGTCGAGCAATTAACAAAAGca                                                                <  1:84356/140‑1 (MQ=255)                                                                           ccccGATTTTTCTATTTCTCTCGGTGCAGACGGTATTGAACGCAAACTGGAAATAGAAAAATCGGTCACTGACGTTGCGGAACTGAACGGTGAATTACGTAATCGGCAGTATCTTGTCGAGCAATTAACAAAAGCACATg                                                            >  2:681143/1‑140 (MQ=255)                                                                           ccccGATTTTTCTATTTCTCTCGGTGCAGACGGTATTGAACGCAAACTGGAAATAGAAAAATCGGTCACTGACGTTGCGGAACTGAACGGTGAATTACGTAATCGGCAGTATCTTGTCGAGCAATTAACAAAAGCACATg                                                            >  1:313101/1‑140 (MQ=255)                                                                           ccccGATTTTTCTATTTCTCTCGGTGCAGACGGTATTGAACGCAAACTGGAAATAGAAAAATCGGTCACTGACGTTGCGGAACTGAACGGTGAATTACGTAATCGGCAGTATCTTGTCGAGCAATTAACAAAAGCACATg                                                            >  1:112440/1‑140 (MQ=255)                                                                           ccccGATTTTTCTATTTCTCTCGGTGCAGACGGTATTGAACGCAAACTGGAAATAGAAAAATCGGTCACTGACGTTGCGGAACTGAACGGTGAATTACGTAATCGGCAGTATCTTGTCGAGCAATTAACAAAAGCACATg                                                            >  1:748268/1‑140 (MQ=255)                                                                              cGATTTTTCTATTTCTCTCGGTGCAGACGGTATTGAACGCAAACTGGAAATAGAAAAATCGGTCACTGACGTTGCGGAACTGAACGGTGAATTACGTAATCGGCAGTATCTTGTCGAGCAATTAACAAAAGCACATGTCa                                                         <  2:313101/140‑1 (MQ=255)                                                                                 tttttCTATTTCTCTCGGTGCAGACGGTATTGAACGCAAACTGGAAATAGAAAAATCGGTCACTGACGTTGCGGAACTGAACGGTGAATTACGTAATCGGCAGTATCTTGTCGAGCAATTAACAAAAGCACATGTCAACg                                                      >  2:975515/1‑140 (MQ=255)                                                                                     tCTATTTCTCTCGGTGCAGACGGTATTGAACGCAAACTGGAAATAGAAAAATCGGTCACTGACGTTGCGGAACTGAACGGTGAATTACGTAATCGGCAGTATCTTGTCGAGCAATTAACAAAAGCACATGTCAACGAtgt                                                  <  1:681143/140‑1 (MQ=255)                                                                                         tttCTCTCGGTGCAGACGGTATTGAACGCAAACTGGAAATAGAAAAATCGGTCACTGACGTTGCGGAACTGAACGGTGAATTACGTAATCGGCAGTATCTTGTCGAGCAATTAACAAAAGCACATGTCAACGATGTGAAt                                              >  2:2911/1‑140 (MQ=255)                                                                                               tcagTGCAGACGGTATTGAACGCAAACTGGAAATAGAAAAATCGGTCACTGACGTTGCGGAACTGAACGGTGAATTACGTAATCGGCAGTATCTTGTCGAGCAATTAACCAAAGCACATGTCAACGATGTGAATTTTACg                                        <  2:1298000/137‑1 (MQ=255)                                                                                               tcGGTGCAGACGGTATTGAACGCAAACTGGAAATAGAAAAATCGGTCACTGACGTTGCGGAACTGAACGGTGAATTACGTAATCGGCAGTATCTTGTCGAGCAATTAACAAAAGCACATGTCAACGATGTGAATTTTACg                                        <  1:1229005/140‑1 (MQ=255)                                                                                               tcGGTGCAGACGGTATTGAACGCAAACTGGAAATAGAAAAATCGGTCACTGACGTTGCGGAACTGAACGGTGAATTACGTAATCGGCAGTATCTTGTCGAGCAATTAACAAAAGCACATGTCAACGATGTGAATTTTACg                                        <  2:1378234/140‑1 (MQ=255)                                                                                               tcGGTGCAGACGGTATTGAACGCAAACTGGAAATAGAAAAATCGGTCACTGACGTTGCGGAACTGAACGGTGAATTACGTAATCGGCAGTATCTTGTCGAGCAATTAACAAAAGCACATGTCAACGATGTGAATTTTACg                                        <  2:1206801/140‑1 (MQ=255)                                                                                               tcGGTGCAGACGGTATTGAACGCAAACTGGAAATAGAAAAATCGGTCACTGACGTTGCGGAACTGAACGGTGAATTACGTAATCGGCAGTATCTTGTCGAGCAATTAACAAAAGCACATGTCAACGATGTGAATTTTACg                                        <  1:256063/140‑1 (MQ=255)                                                                                               tcGGTGCAGACGGTATTGAACGCAAACTGGAAATAGAAAAATCGGTCACTGACGTTGCGGAACTGAACGGTGAATTACGTAATCGGCAGTATCTTGTCGAGCAATTAACAAAAGCACATGTCAACGATGTGAATTTTACg                                        <  2:457167/140‑1 (MQ=255)                                                                                                 ggTGCAGACGGTATTGAACGCAAACTGGAAATAGAAAAATCGGTCACTGACGTTGCGGAACTGAACGGTGAATTACGTAATCGGCAGTATCTTGTCGAGCAATTAACAAAAGCACATGTCAACGATGTGAATTTTACGcc                                      <  2:100539/140‑1 (MQ=255)                                                                                                      aGACGGTATTGAACGCAAACTGGAAATAGAAAAATCGGTCACTGACGTTGCGGAACTGAACGGTGAATTACGTAATCGGCAGTATCTTGTCGAGCAATTAACAAAAGCACATGTCAACGATGTGAATTTTACGCCGTTTa                                 <  2:374398/140‑1 (MQ=255)                                                                                                        aCGGTATTGAACGCAAACTGGAAATAGAAAAATCGGTCACTGACGTTGCGGAACTGAACGGTGAATTACGTAATCGGCAGTATCTTGTCGAGCAATTAACAAAAGCACATGTCAACGATGTGAATTTTACGCCGTTTaaa                               >  1:209407/1‑140 (MQ=255)                                                                                                          ggTATTGAACGCAAACTGGAAATAGAAAAATCGGTCACTGACGTTGCGGAACTGAACGGTGAATTACGTAATCGGCAGTATCTTGTCGAGCAATTAACAAAAGCACATGTCAACGATGTGAATTTTACGCCGTTTAAata                             <  2:112440/140‑1 (MQ=255)                                                                                                             aTTGAACGCAAACTGGAAATAGAAAAATCGGTCACTGACGTTGCGGAACTGAACGGTGAATTACGTAATCGGCAGTATCTTGTCGAGCAATTAACAAAAGCACATGTCAACGATGTGAATTTTACGCCGTTTAAATATCa                          >  2:1356787/1‑140 (MQ=255)                                                                                                             aTTGAACGCAAACTGGAAATAGAAAAATCGGTCACTGACGGTGCGGAACTGAACGGTGAATTACGTAATCGGCAGTATCTTGTCGAGCAATTAACAAAAGCACATGTCAACGATGTGAATTTTACGCCGTTTAAATATCa                          >  2:1036658/1‑140 (MQ=255)                                                                                                                    gCAAACTGGAAATAGAAAAATCGGTCACTGACGTTGCGGAACTGAACGGTGAATTACGTAATCGGCAGTATCTTGTCGAGCAATTAACAAAAGCACATGTCAACGATGTGAATTTTACGCCGTTTAAATATCAGTTAAGc                   <  1:39027/140‑1 (MQ=255)                                                                                                                      aaaCTGGAAATAGAAAAATCGGTCACTGACGTTGCGGAACTGAACGGTGAATTACGTAATCGGCAGTATCTTGTCGAGCAATTAACAAAAGCACATGTCAACGATGTGAATTTTACGCCGTTTAAata                             >  1:426194/1‑128 (MQ=255)                                                                                                                      aaaCTGGAAATAGAAAAATCGGTCACTGACGTTGCGGAACTGAACGGTGAATTACGTAATCGGCAGTATCTTGTCGAGCAATTAACAAAAGCACATGTCAACGATGTGAATTTTACGCCGTTTAAata                             <  2:426194/128‑1 (MQ=255)                                                                                                                      aaaCTGGAAATAGAAAAATCGGTCACTGACGTTGCGGAACTGAACGGTGAATTACGTAATCGGCAGTATCTTGTCGAGCAATTAACAAAAGCACATGTCAACGATGTGAATTTTACGCCGTTTAAATATCAGTTAAGccc                 >  2:13769/1‑140 (MQ=255)                                                                                                                      aaaCTGGAAATAGAAAAATCGGTCACTGACGTTGCGGAACTGAACGGTGAATTACGTAATCGGCAGTATCTTGTCGAGCAATTAACAAAAGCACATGTCAACGATGTGAATTTTACGCCGTTTAAATATCAGTTAAGccc                 >  2:666326/1‑140 (MQ=255)                                                                                                                      aaaCTGGAAATAGAAAAATCGGTCACTGACGTTGCGGAACTGAACGGTGAATTACGTAATCGGCAGTATCTTGTCGAGCAATTAACAAAAGCACATGTCAACGATGTGAATTTTACGCCGTTTAAATATCAGTTAAGccc                 >  1:1327557/1‑140 (MQ=255)                                                                                                                      aaaCTGGAAATAGAAAAATCGGTCACTGACGTTGCGGAACTGAACGGTGAATTACGTAATCGGCAGTATCTTGTCGAGCAATTAACAAAAGCACATGTCAACGATGTGAATTTTACGCCGTTTAAATATCAGTTAAGccc                 >  2:1267823/1‑140 (MQ=255)                                                                                                                      aaaCTGGAAATAGAAAAATCGGTCACTGACGTTGCGGAACTGAACGGTGAATTACGTAATCGGCAGTATCTTGTCGAGCAATTAACAAAAGCACATGTCAACGATGTGAATTTTACGCCGTTTAAATATCAGTTAAGccc                 >  1:1090063/1‑140 (MQ=255)                                                                                                                      aaaCTGGAAATAGAAAAATCGGTCACTGACGTTGCGGAACTGAACGGTGAATTACGTAATCGGCAGTATCTTGTCGAGCAATTAACAAAAGCACATGTCAACGATGTGAATTTTACGCCGTTTAAATATCAGTTAAGc                   <  2:164895/138‑1 (MQ=255)                                                                                                                      aaaCTGGAAATAGAAAAATCGGTCACTGACGTTGCGGAACTGAACGGTGAATTACGTAATCGGCAGTATCTTGTCGAGCAATTAACAAAAGCACATGTCAACGATGTGAATTTTACGCCGTTTAAATATCAGTTAAGc                   >  1:164895/1‑138 (MQ=255)                                                                                                                        aCTGGAAATAGAAAAATCGGTCACTGACGTTGCGGAACTGAACGGTGAATTACGTAATCGGCAGTATCTTGTCGAGCAATTAACAAAAGCACATGTCAACGATGTGAATTTTACGCCGTTTAAATATCAGTTAAGCCCGt               >  1:125384/1‑140 (MQ=255)                                                                                                                                  gAAAAATCGGTCACTGACGTTGCGGAACTGAACGGTGAATTACGTAATCGGCAGTATCTTGTCGAGCAATTAACAAAAGCACATGTCAACGATGTGAATTTTACGCCGTTTAAATATCAGTTAAGCCCGTCATTGCCAGt     >  1:167826/1‑140 (MQ=255)                                                                                                                                  gAAAAATCGGTCACTGACGTTGCGGAACTGAACGGTGAATTACGTAATCGGCAGTATCTTGTCGAGCAATTAACAAAAGCACATGTCAACGATGTGAATTTTACGCCGTTTAAATATCAGTTAAGCCCGTCATTGCCAGt     >  1:1360490/1‑140 (MQ=255)                                                                                                                                    aaaaTCGGTCACTGACGTTGCGGAACTGAACGGTGAATTACGTAATCGGCAGTATCTTGTCGAGCAATTAACAAAAGCACATGTCAACGATGTGAATTTTACGCCGTTTAAATATCAGTTAAGCCCGTCATTGCCAGTGa   >  1:790342/1‑140 (MQ=255)                                                                                                                                    aaaaTCGGTCACTGACGTTGCGGAACTGAACGGTGAATTACGTAATCGGCAGTATCTTGTCGAGCAATTAACAAAAGCACATGTCAACGATGTGAATTTTACGCCGTTTAAATATCAGTTAAGCCCGTCATTGCCAGTGa   <  1:975515/140‑1 (MQ=255)                                                                                                                                     aaaTCGGTCACTGACGTTGCGGAACTGAACGGTGAATTACGTAATCGGCAGTATCTTGTCGAGCAATTAACAAAAGCACATGTCAACGATGTGAATTTTACGCCGTTTAAATATCAGTTAAGCCCGTCATTGCCAGTGaa  <  1:1344237/140‑1 (MQ=255)                                                                                                                                        |                                                                                                                                         TATTCACTCGACATTGCCAACGCGGCAGGAATTAAAAAGCCCGTTTACAGTAATGGTCAGGCCGTTAAAGATGACCCCGATTTTTCTATTTCTCTCGGTGCAGACGGTATTGAACGCAAACTGGAAATAGAAAAAGCGGTCACTGACGTTGCGGAACTGAACGGTGAATTACGTAATCGGCAGTATCTTGTCGAGCAATTAACAAAAGCACATGTCAACGATGTGAATTTTACGCCGTTTAAATATCAGTTAAGCCCGTCATTGCCAGTGAA  >  NC_000913/618977‑619248

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑