Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	133,173	C→T	A520V (GCA→GTA)	acnB →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	133,173	0	C	T	100.0%	218.1 / NA	67	A520V (GCA→GTA)	acnB	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (34/33);  total (34/33)

GGTGACGGCGTCATTCACTCCTGGCTGAACCGTATGCTGCTGCCGGATACCGTCGGTACCGGTGGTGACTCCCATACCCGTTTCCCGATCGGTATCTCTTTCCCGGCGGGTTCTGGTCTGGTGGCGTTTGCTGCCGCAACTGGCGTAATGCCGCTTGATATGCCGGAATCCGTTCTGGTGCGCTTCAAAGGCAAAATGCAGCCGGGCATCACCCTGCGCGATCTGGTACACGCTATTCCGCTGTATGCGATCAAACAAGGTCTGCTGACCG  >  NC_000913/133037‑133307                                                                                                                                         |                                                                                                                                       ggTGACGGCGTCATTCACTCCTGGCTGAACCGTATGCTGCTGCCGGATACCGTCGGTACCGGTGGTGACTCCCATACCCGTTTCCCGATCGGTATCTCTTTCCCGGCGGGTTCTGGTCTGGTGGCGTTTGCTGCCGTAAc                                                                                                                                     <  2:581178/140‑1 (MQ=255) ggTGACGGCGTCATTCACTCCTGGCTGAACCGTATGCTGCTGCCGGATACCGTCGGTACCGGTGGTGACTCCCATACCCGTTTCCCGATCGGTATCTCTTTCCCGGCGGGTTCTGGTCTGGTGGCGTTTGCTGCCGTAAc                                                                                                                                     <  1:596104/140‑1 (MQ=255)     aCGGCGTCATTCACTCCTGGCTGAACCGTATGCTGCTGCCGGATACCGTCGGTACCGGTGGTGACTCCCATACCCGTTTCCCGATCGGTATCTCTTTCCCGGCGGGTTCTGGTCTGGTGGCGTTTGCTGCCGTAACTGGc                                                                                                                                 >  2:865055/1‑140 (MQ=255)     aCGGCGTCATTCACTCCTGGCTGAACCGTATGCTGCTGCCGGATACCGTCGGTACCGGTGGTGACTCCCATACCCGTTTCCCGATCGGTATCTCTTTCCCGGCGGGTTCTGGTCTGGTGGCGTTTGCTGCCGTAACTGGc                                                                                                                                 <  2:1119704/140‑1 (MQ=255)        gCGTCATTCACTCCTGGCTGAACCGTATGCTGCTGCCGGATACCGTCGGTACCGGTGGTGACTCCCATACCCGTTTCCCGATCGGTATCTCTTTCCCGGCGGGTTCTGGTCTGGTGGCGTTTGCTGCCGTAACTGGCGTa                                                                                                                              >  2:339263/1‑140 (MQ=255)         cGTCATTCACTCCTGGCTGAACCGTATGCTGCTGCCGGATACCGTCGGTACCGGTGGTGACTCCCATACCCGTTTCCCGATCGGTATCTCTTTCCCGGCGGGTTCTGGTCTGGTGGCGTTTGCTGCCGTAACTGGCGTaa                                                                                                                             <  1:339263/140‑1 (MQ=255)         cGTCATTCACTCCTGGCTGAACCGTATGCTGCTGCCGGATACCGTCGGTACCGGTGGTGACTCCCATACCCGTTTCCCGATCGGTATCTCTTTCCCGGCGGGTTCTGGTCTGGTGGCGTTTGCTGCCGTAACTGGCGTaa                                                                                                                             <  1:698179/140‑1 (MQ=255)          gTCATTCACTCCTGGCTGAACCGTATGCTGCTGCCGGATACCGTCGGTACCGGTGGTGACTCCCATACCCGTTTCCCGATCGGTATCTCTTTCCCGGCGGGGTCTGGTCCGGGGGCGTTTGCTGCCGTAACTGGCGTAAt                                                                                                                            >  2:679469/1‑140 (MQ=255)             aTTCACTCCTGGCTGAACCGTATGCTGCTGCCGGATACCGTCGGTACCGGTGGTGACTCCCATACCCGTTTCCCGATCGGTATCTCTTTCCCGGCGGGTTCTGGTCTGGTGGCGTTTGCTGCCGTAACTGGCGTAATGcc                                                                                                                         <  1:586637/140‑1 (MQ=255)                cACTCCTGGCTGAACCGTATGCTGCTGCCGGATACCGTCGGTACCGGTGGTGACTCCCATACCCGTTTCCCGATCGGTATCTCTTTCCCGGCGGGTTCTGGTCTGGTGGCGTTTGCTGCCGTAACTGGCGTAATGCCGCt                                                                                                                      <  1:1327583/140‑1 (MQ=255)                   tCCTGGCTGAACCGTATGCTGCTGCCGGATACCGTCGGTACCGGTGGTGACTCCCATACCCGTTTCCCGATCGGTATCTCTTTCCCGGCGGGTTCTGGTCTGGTGGCGTTTGCTGCCGTAACTGGCGTAATGCCGCTTGa                                                                                                                   >  1:251734/1‑140 (MQ=255)                        gCTGAACCGTATGCTGCTGCCGGATACCGTCGGTACCGGTGGTGACTCCCATACCCGTTTCCCGATCGGTATCTCTTTCCCGGCGGGTTCTGGTCTGGTGGCGTTTGCTGCCGTAACTGGCGTAATGCCGCTTGATATGc                                                                                                              >  1:1172286/1‑140 (MQ=255)                            aaCCGTATGCTGCTGCCGGATACCGTCGGTACCGGTGGTGACTCCCATACCCGTTTCCCGATCGGTATCTCTTTCCCGGCGGGTTCTGGTCTGGTGGCGTTTGCTGCCGTAACTGGCGTAATGCCGCTTGATATGCCGGa                                                                                                          <  1:1369809/140‑1 (MQ=255)                             aCCGTATGCTGCTGCCGGATACCGTCGGTACCGGTGGTGACTCCCATACCCGTTTCCCGATCGGTATCTCTTTCCCGGCGGGGTCTGGTCTGGTGGCGTTTGCTGCCGTAACTGGCGTAATGCCGCTTGATATGCCGGaa                                                                                                         >  2:1260967/1‑140 (MQ=255)                             aCCGTATGCTGCTGCCGGATACCGTCGGTACCGGTGGTGACTCCCATACCCGTTTCCCGATCGGTATCTCTTTCCCGGCGGATTCTGGTCTGGTGGCGTTTGCTGCCGTAACTGGCGTAATGCCGCTTGATATGCCGGaa                                                                                                         >  2:1243458/1‑140 (MQ=255)                                 tATGCTGCTGCCGGATACCGTCGGTACCGGTGGTGACTCCCATACCCGTTTCCCGATCGGTATCTCTTTCCCGGCGGGTTCTGGTCTGGTGGCGTTTGCTGCCGTAACTGGCGTAATGCCGCTTGATATGCCGGAATCCg                                                                                                     >  2:286382/1‑140 (MQ=255)                                 tATGCTGCTGCCGGATACCGTCGGTACCGGTGGTGACTCCCATACCCGTTTCCCGATCGGTATCTCTTTCCCGGCGGGTTCTGGTCTGGTGGCGTTTGCTGCCGTAACTGGCGTAATGCCGCTTGATATGCCGGAATCCg                                                                                                     >  2:448910/1‑140 (MQ=255)                                   tgctgctgcCGGATACCGTCGGTACCGGTGGTGACTCCCATACCCGTTTCCCGATCGGTATCTCTTTCCCGGCGGGGTCTGGTCTGGTGGCGTTTGCTGCCGTAACTGGCGTAATGCCGCTTGATATGCCGGAATCCGtt                                                                                                   >  2:580973/1‑140 (MQ=255)                                      tgctgcCGGATACCGTCGGTACCGGTGGTGACTCCCATACCCGTTTCCCGATCGGTATCTCTTTCCCGGCGGGTTCTGGTCTGGTGGCGTTTGCTGCCGTAACTGGCGTAATGCCGCTTGATATGCCg                                                                                                            <  2:130637/128‑1 (MQ=255)                                      tgctgcCGGATACCGTCGGTACCGGTGGTGACTCCCATACCCGTTTCCCGATCGGTATCTCTTTCCCGGCGGGTTCTGGTCTGGTGGCGTTTGCTGCCGTAACTGGCGTAATGCCGCTTGATATGCCg                                                                                                            >  1:130637/1‑128 (MQ=255)                                       gctgcCGGATACCGTCGGTACCGGTGGTGACTCCCATACCCGTTTCCCGATCGGTATCTCTTTCCCGGCGGGTTCTGGTCTGGTGGCGTTTGCTGCCGTAACTGGCGTAATGCCGCTTGATATGCCGGAATCCGTTCTgg                                                                                               <  2:376827/140‑1 (MQ=255)                                         tgcCGGATACCGTCGGTACCGGTGGTGACTCCCATACCCGTTTCCCGATCGGTATCTCTTTCCCGGCGGGTTTTGGTCTGGTGGCGTTTGCTGCCGCAACTGGGGCAATGCCGCTTGATATGCCGGAATCCGTTCTgggg                                                                                             >  2:1109617/1‑138 (MQ=255)                                           ccGGATACCGTCGGTACCGGTGGTGACTCCCATACCCGTTTCCCGATCGGTATCTCTTTCCCGGCGGGGTCTGGTCTGGTGGCGTTTGCTGCCGTAACTGGCGTAATGCCGCTTGATATGCCGGAATCCGTTCTGGTgcg                                                                                           >  2:43814/1‑140 (MQ=255)                                                     tCGGTACCGGTGGTGACTCCCATACCCGTTTCCCGATCGGTATCTCTTTCCCGGCGGGTTCTGGTCTGGTGGCGTTTGCTGCCGTAACTGGCGTAATGCCGCTTGATATGCCGGAATCCGTTCTGGTGCGCTTCAAAGGc                                                                                 >  1:780500/1‑140 (MQ=255)                                                      cGGTACCGGTGGTGACTCCCATACCCGTTTCCCGATCGGTATCTCTTTCCCGGCGGGTTCTGGTCTGGTGGCGTTTGCTGCCGTAACTGGCGTAATGCCGCTTGATATGCCGGAATCCGTTCTGGTGCGCTTCAAAGGCa                                                                                >  2:940965/1‑140 (MQ=255)                                                             ggtggtGACTCCCATACCCGTTTCCCGATCGGTATCTCTTTCCCGGCGGGTTCTGGTCTGGTGGCGTTTGCTGCCGTAACTGGCGTAATGCCGCTTGATATGCCGGAATCCGTTCTGGTGCGCTTCAAAGGCAAAATGCa                                                                         >  2:900295/1‑140 (MQ=255)                                                              gtggtgACTCCCATACCCGTTTCCCGATCGGTATCTCTTTCCCGGCGGGTTCTGGTCTGGTGGCGTTTGCTGCCGTAACTGGCGTAATGCCGCTTGATATGCCGGAATCCGTTCTGGTGCGCTTCAAAGGCAAAATGCAg                                                                        <  1:900295/140‑1 (MQ=255)                                                               tggtgACTCCCATACCCGTTTCCCGATCGGTATCTCTTTCCCGGCGGGTTCTGGTCTGGTGGCGTTTGCTGCCGTAACTGGCGTAATGCCGCTTGATATGCCGGAATCCGTTCTGGTGCGCTTCAAAGGGAAAATGCAGc                                                                       >  1:972492/1‑140 (MQ=255)                                                               tggtgACTCCCATACCCGTTTCCCGATCGGTATCTCTTTCCCGGCGGGTTCTGGGCTAGGGGCGCTTTCTTCCGTAACTGGCGTAATGCCGCTTGATATTACGGAATTCGTTCTGGGGCGCCTCAAAGGCAAAATGCAGc                                                                       >  2:978199/1‑140 (MQ=255)                                                                ggtgACTCCCATACCCGTTTCCCGATCGGTATCTCTTTCCCGGCGGGTTCTGGTCTGGTGGCGTTTGCTGCCGTAACTGGCGTAATGCCGCTTGATATGCCGGAATCCGTTCTGGTGCGCTTCAAAGGCAAAATGCAGcc                                                                      <  2:780500/140‑1 (MQ=255)                                                                   gACTCCCATACCCGTTTCCCGATCGGTATCTCTTTCCCGGCGGGTTCTGGTCTGGTGGCGTTTGCTGCCGTAACTGGCGTAATGCCGCTTGATATGCCGGAATCCGTTCTGGTGCGCTTCAAAGGCAAAATGCAg                                                                        <  2:1381833/135‑1 (MQ=255)                                                                   gACTCCCATACCCGTTTCCCGATCGGTATCTCTTTCCCGGCGGGTTCTGGTCTGGTGGCGTTTGCTGCCGTAACTGGCGTAATGCCGCTTGATATGCCGGAATCCGTTCTGGTGCGCTTCAAAGGCAAAATGCAg                                                                        >  1:1381833/1‑135 (MQ=255)                                                                     cTCCCATACCCGTTTCCCGATCGGTATCTCTTTCCCGGCGGGTTCTGGTCTGGTGGCGTTTGCTGCCGTAACTGGCGTAATGCCGCTTGATATGCCGGAATCCGTTCTGGTGCGCTTCAAAGGCAAAATGCAGCCGGGCa                                                                 <  2:963460/140‑1 (MQ=255)                                                                        ccATACCCGTTTCCCGATCGGTATCTCTTTCCCGGCGGGTTCTGGTCTGGTGGCGTTTGCTGCCGTAACTGGCGTAATGCCGCTTGATATGCCGGAATCCGTTCTGGTGCGCTTCAAAGGCAAAATGCAGCCGGGCATCa                                                              >  2:463089/1‑140 (MQ=255)                                                                               cGTTTCCCGATCGGTATCTCTTTCCCGGCGGGTTCTGGTCTGGTGGCGTTTGCTGCCGTAACTGGCGTAATGCCGCTTGATATGCCGGAATCCGTTCTGGTGCGCTTCAAAGGCAAAATGCAGCCGGGCATCACCCTgcg                                                       <  1:679469/140‑1 (MQ=255)                                                                               cGTTTCCCGATCGGTATCTCTTTCCCGGCGGGTTCTGGTCTGGTGGCGTTTGCTGCCGTAACTGGCGTAATGCCGCTTGATATGCCGGAATCCGTTCTGGTGCGCTTCAAAGGCAAAATGCAGCCGGGCATCACCCTgcg                                                       <  1:875787/140‑1 (MQ=255)                                                                                 tttCCCGATCGGTATCTCTTTCCCGGCGGGTTCTGGTCTGGTGGCGTTTGCTGCCGTAACTGGCGTAATGCCGCTTGATATGCCGGAATCCGTTCTGGTGCGCTTCAAAGGCAAAATGCAGCCGGGCATCACCCTGcgcg                                                     >  2:212039/1‑140 (MQ=255)                                                                                 tttCCCGATCGGTATCTCTTTCCCGGCGGGTTCTGGTCTGGTGGCGTTTGCTGCCGTAACTGGCGTAATGCCGCTTGATATGCCGGAATCCGTTCTGGTGCGCTTCAAAGGCAAAATGCAGCCGGGCATCACCCTGcgcg                                                     <  1:30446/140‑1 (MQ=255)                                                                                  ttCCCGATCGGTATCTCTTTCCCGGCGGGTTCTGGTCTGGTGGCGTTTGCTGCCGTAACTGGCGTTATGCCGCTTGATATGCCGGAATCCGTTCTGGTGCGCTTCAAAGGCAAAATGCAGCCGGGCATCACCCTGCGCGa                                                    >  2:1249199/1‑140 (MQ=255)                                                                                  ttCCCGATCGGTATCTCTTTCCCGGCGGGTTCTGGTCTGGTGGCGTTTGCTGCCGTAACTGGCGTAATGCCGCTTGATATGCCGGAATCCGTTCTGGTGCGCTTCAAAGGCAAAATGCAGCCGGGCATCACCCTGCGCGa                                                    >  1:1361133/1‑140 (MQ=255)                                                                                  ttCCCGATCGGTATCTCTTTCCCGGCGGGTTCTGGTCTGGTGGCGTTTGCTGCCGTAACTGGCGTAATGCCGCTTGATATGCCGGAATCCGTTCTGGTGCGCTTCAAAGGCAAAATGCAGCCGGGCATCACCCTGCGCGa                                                    >  2:1206154/1‑140 (MQ=255)                                                                                   tCCCGATCGGTATCTCTTTCCCGGCGGGTTCTGGTCTGGTGGCGTTTGCTGCCGTAACTGGCGTAATGCCGCTTGATATGCCGGAATCCGTTCTGGTGCGCTTCAAAGGCAAAATGCAGCCGGGCATCACCCTGCGCGAt                                                   <  1:1206154/140‑1 (MQ=255)                                                                                   tCCCGATCGGTATCTCTTTCCCGGCGGGTTCTGGTCTGGTGGCGTTTGCTGCCGTAACTGGCGTAATGCCGCTTGATATGCCGGAATCCGTTCTGGTGCGCTTCAAAGGCAAAATGCAGCCGGGCATCACCCTGCGCGAt                                                   >  1:491414/1‑140 (MQ=255)                                                                                        aTCGGTATCTCTTTCCCGGCGGGTTCTGGTCTGGTGGCGTTTGCTGCCGTAACTGGCGTAATGCCGCTTGATATTCCGGAATCCGTTCTGGTGCGCTTCAAAGGCAAAATGCAGCCGGGCATCACCCTGCGCGATCTggg                                              >  2:1111880/1‑139 (MQ=255)                                                                                        aTCGGTATCTCTTTCCCGGCGGGTTCTGGTCTGGTGGCGTTTGCTGCCGTAACTGGCGTAATGCCGCTTGATATGCCGGAATCCGTTCTGGTGCGCTTCAAAGGCAAAATGCAGCCGGGCATCACCCTGCGCGATCTGGt                                              <  1:987402/140‑1 (MQ=255)                                                                                        aTCGGTATCTCTTTCCCGGCGGGTTCTGGTCTGGTGGCGTTTGCTGCCGTAACTGGCGTAATGCCGCTTGATATGCCGGAATCCGTTCTGGTGCGCTTCAAAGGCAAAATGCAGCCGGGCATCACCCTGCGCGATCTGGt                                              <  2:251734/140‑1 (MQ=255)                                                                                        aTCGGTATCTCTTTCCCGGCGGGTTCTGGTCTGGTGGCGTTTGCTGCCGTAACTGGCGTAATGCCGCTTGATATGCCGGAATCCGTTCTGGTGCGCTTCAAAGGCAAAATGCAGCCGGGCATCACCCTGCGCGATCTGGt                                              >  1:570815/1‑140 (MQ=255)                                                                                           ggTATCTCTTTCCCGGCGGGTTCTGGTCTGGTGGCGTTTGCTGCCGTAACTGGCGTAATGCCGCTTGATATGCCGGAATCCGTTCTGGTGCGCTTCAAAGGCAAAATGCAGCCGGGCATCACCCTGCGCGATCTGGTaca                                           <  1:286382/140‑1 (MQ=255)                                                                                             tATCTCTTTCCCGGCGGGTTCTGGTCTGGTGGCGTTTGCTGCCGTAACTGGCGTAATGCCGCTTGATATGCCGGAATCCGTTCTGGTGCGCTTCAAAGGCAAAATGCAGCCGGGCATCACCCTGCGCGATCTGGTACACg                                         <  2:570815/140‑1 (MQ=255)                                                                                                 tctTTCCCGGCGGGTTCTGGTCTGGTGGCGTTTGCTGCCGTAACTGGCGTAATGCCGCTTGATATGCCGGAATCCGTTCTGGTGCGCTTCAAAGGCAAAATGCAGCCGGGCATCACCCTGCGCGATCTGGTACACGCTAt                                     <  1:1055315/140‑1 (MQ=255)                                                                                                    ttCCCGGCGGGTTCTGGTCTGGTGGCGTTTGCTGCCGTAACTGGCGTAATGCCGCTTGATATGCCGGAATCCGTTCTGGTGCGCTTCAAAGGCAAAATGCAGCCGGGCATCACCCTGCGCGATCTGGTACACGCTATTcc                                  >  1:612694/1‑140 (MQ=255)                                                                                                       ccGGCGGGTTCTGGTCTGGTGGCGTTTGCTGCCGTAACTGGCGTAATGCCGCTTGATATGCCGGAATCCGTTCTGGTGCGCTTCAAAGGCAAAATGCAGCCGGGCATCACCCTGCGCGATCTGGTACACGCTATTCCGCt                               >  2:519368/1‑140 (MQ=255)                                                                                                         ggcggGTTCTGGTCTGGTGGCGTTTGCTGCCGTAACTGGCGTAATGCCGCTTGATATGCCGGAATCCGTTCTGGTGCGCTTCAAAGGCAAAATGCAGCCGGGCATCACCCTGCGCGATCTGGTACACGCTATTCCGCTGt                             >  2:413512/1‑140 (MQ=255)                                                                                                          gcggGTTCTGGTCTGGTGGCGTTTGCTGCCGTAACTGGCGTAATGCCGCTTGATATGCCGGAATCCGTTCTGGTGCGCTTCAAAGGCAAAATGCAGCCGGGCATCACCCTGCGCGATCTGGTACACGCTATTCCGCTGTa                            >  1:1344354/1‑140 (MQ=255)                                                                                                          gcggGTTCTGGTCTGGTGGCGTTTGCTGCCGTAACTGGCGTAATGCCGCTTGATATGCCGGAATCCGTTCTGGTGCGCTTCAAAGGCAAAATGCAGCCGGGCATCACCCTGCGCGATCTGGTACACGCTATTCCGCTGTa                            <  1:620815/140‑1 (MQ=255)                                                                                                           cggGTTCTGGTCTGGTGGCGTTTGCTGCCGTAACTGGCGTAATGCCGCTTGATATGCCGGAATCCGTTCTGGTGCGCTTCAAAGGCAAAATGCAGCCGGGCATCACCCTGCGCGATCTGGTACACGCTATTCCGCTGTAt                           >  1:1282585/1‑140 (MQ=255)                                                                                                           cggGTTCTGGTCTGGTGGCGTTTGCTGCCGTAACTGGCGTAATGCCGCTTGATATGCCGGAATCCGTTCTGGTGCGCTTCAAAGGCAAAATGCAGCCGGGCATCACCCTGCGCGATCTGGTACACGCTATTCCGCTGTAt                           >  2:584009/1‑140 (MQ=255)                                                                                                           cggGTTCTGGTCTGGTGGCGTTTGCTGCCGTAACTGGCGTAATGCCGCTTGATATGCCGGAATCCGTTCTGGTGCGCTTCAAAGGCAAAATGCAGCCGGGCATCACCCTGCGCGATCTGGTACACGCTATTCCGCTGTAt                           >  1:120777/1‑140 (MQ=255)                                                                                                              gTTCTGGTCTGGTGGCGTTTGCTGCCGTAACTGGCGTAATGCCGCTTGATATGCCGGAATCCGTTCTGGTGCGCTTCAAAGGCAAAATGCAGCCGGGCATCACCCTGCGCGATCTGGTACACGCTATTCCGCTGTATGCg                        <  1:413512/140‑1 (MQ=255)                                                                                                                tctggtctggTGGCGTTTGCTGCCGTAACTGGCGTAATGCCGCTTGATATGCCGGAATCCGTTCTGGTGCGCTTCAAAGGCAAAATGCAGCCGGGCATCACCCTGCGCGATCTGGTACACGCTATTCCGCTGTATGCGAt                      <  1:820370/140‑1 (MQ=255)                                                                                                                tctggtctggTGGCGTTTGCTGCCGTAACTGGCGTAATGCCGCTTGATATGCCGGAATCCGTTCTGGTGCGCTTCAAAGGCAAAATGCAGCCGGGCATCACCCTGCGCGATCTGGTACACGCTATTCCGCTGTATGCGAt                      <  1:212039/140‑1 (MQ=255)                                                                                                                   ggtctggtGGCGTTTGCTGCCGTAACTGGCGTAATGCCGCTTGATATGCCGGAATCCGTTCTGGTGCGCTTCAAAGGCAAAATGCAGCCGGGCATCACCCTGCGCGATCTGGTACACGCTATTCCGCTGTATGCGATCaa                   <  1:463089/140‑1 (MQ=255)                                                                                                                    gtctggtGGCGTTTGCTGCCGTAACTGGCGTAATGCCGCTTGATATGCCGGAATCCGTTCTGGTGCGCTTCAAAGGCAAAATGCAGCCGGGCATCACCCTGCGCGATCTGGTACACGCTATTCCGCTGTATGCGATCaaa                  <  1:940965/140‑1 (MQ=255)                                                                                                                        ggtggCGTTTGCTGCCGTAACTGGCGTAATGCCGCTTGATATGCCGGAATCCGTTCTGGTGCGCTTCAAAGGCAAAATGCAGCCGGGCATCACCCTGCGCGATCTGGTACACGCTATTCCGCTGTATGCGATCAAACAAg              <  1:584009/140‑1 (MQ=255)                                                                                                                        ggtggCGTTTGCTGCCGTAACTGGCGTAATGCCGCTTGATATGCCGGAATCCGTTCTGGTGCGCTTCAAAGGCAAAATGCAGCCGGGCATCACCCTGCGCGATCTGGTACACGCTATTCCGCTGTATGCGATCAAACAAg              >  1:1067799/1‑140 (MQ=255)                                                                                                                          tggCGTTTGCTGCCGTAACTGGCGTAATGCCGCTTGATATGCCGGAATCCGTTCTGGTGCGCTTCAAAGGCAAAATGCAGCCGGGCATCACCCTGCGCGATCTGGTACACGCTATTCCGCTGTATGCGATCAAACAAGGt            <  1:43814/140‑1 (MQ=255)                                                                                                                            gCGTTTGCTGCCGTAACTGGCGTAATGCCGCTTGATATGCCGGAATCCGTTCTGGTGCGCTTCAAAGGCAAAATGCAGCCGGGCATCACCCTGCGCGATCTGGTACACGCTATTCCGCTGTATGCGATCAAACAAGGTct          >  1:1027723/1‑140 (MQ=255)                                                                                                                              gTTTGCTGCCGTAACTGGCGTAATGCCGCTTGATATGCCGGAATCCGTTCTGGTGCGCTTCAAAGGCAAAATGCAGCCGGGCATCACCCTGCGCGATCTGGTACACGCTATTCCGCTGTATGCGATCAAACAAGGt            <  1:1196014/136‑1 (MQ=255)                                                                                                                              gTTTGCTGCCGTAACTGGCGTAATGCCGCTTGATATGCCGGAATCCGTTCTGGTGCGCTTCAAAGGCAAAATGCAGCCGGGCATCACCCTGCGCGATCTGGTACACGCTATTCCGCTGTATGCGATCAAACAAGGt            >  2:1196014/1‑136 (MQ=255)                                                                                                                               tttGCTGCCGTAACTGGCGAAATGACGCTTGATATGCCGGAATCCGTTCTGGTGCGCTTCAAAGGCAAAATGCAGCCGGGCATCACCCTGCGCGATCTGGTACACGCTATTCCGCTGTATGCGATCAAACAAGGTctgct       >  2:55977/1‑140 (MQ=255)                                                                                                                                    tgcCGTAACTGGCGTAATGCCGCTTGATATGCCGGAATCCGTTCTGGTGCGCTTCAAAGGCAAAATGCAGCCGGGCATCACCCTGCGCGATCTGGTACACGCTATTCCGCTGTATGCGATCAAACAAGGTCTGCTGACCg  <  2:1361133/140‑1 (MQ=255)                                                                                                                                         |                                                                                                                                       GGTGACGGCGTCATTCACTCCTGGCTGAACCGTATGCTGCTGCCGGATACCGTCGGTACCGGTGGTGACTCCCATACCCGTTTCCCGATCGGTATCTCTTTCCCGGCGGGTTCTGGTCTGGTGGCGTTTGCTGCCGCAACTGGCGTAATGCCGCTTGATATGCCGGAATCCGTTCTGGTGCGCTTCAAAGGCAAAATGCAGCCGGGCATCACCCTGCGCGATCTGGTACACGCTATTCCGCTGTATGCGATCAAACAAGGTCTGCTGACCG  >  NC_000913/133037‑133307

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑