Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,056,632	T→C	S124S (AGT→AGC)	torT →	periplasmic trimethylamine‑N‑oxide binding protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,056,632	0	T	C	100.0%	301.5 / NA	81	S124S (AGT→AGC)	torT	periplasmic trimethylamine‑N‑oxide binding protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (40/41);  total (40/41)

TGCTGGAGGCAGGCGGCTACAGCCAGTTGGCTACCCAGCAAGCACAAATCGACCAGTGTAAACAGTGGGGCGCAGAGGCCATTTTGCTCGGTAGTAGCACGACCTCATTTCCCGACCTGCAAAAGCAGGTAGCAAGTCTGCCGGTGATCGAACTGGTAAATGCTATTGATGCTCCCCAGGTGAAAAGCCGCGTTGGTGTGCCCTGGTTTCAGATGGGCTATCAACCGGGGCGATATCTGGTGCAATGGGCGCACGGTAAACCACTGAATGTG  >  NC_000913/1056496‑1056767                                                                                                                                         |                                                                                                                                        tGCTGGAGGCAGGCGGCTACAGCCAGTTGGCTACCCAGCAAGCACAAATCGACCAGTGTAAACAGTGGGGCGCAGAGGCCATTTTGCTCGGTAGTAGCCCGACCTCATTTCCCGACCTGCAAAAGCAGGTAGCAAGCCtc                                                                                                                                      >  2:748522/1‑139 (MQ=255)       aggcaggcGGCTACAGCCAGTTGGCTACCCAGCAAGCACAAATCGACCAGTGTAAACAGTGGGGCGCAGAGGCCATTTTGCTCGGTAGTAGCACGACCTCATTTCCCGACCTGCAAAAGCAGGTAGCAAGCCTGCCGGTg                                                                                                                                <  1:577647/140‑1 (MQ=255)           aggcGGCTACAGCCAGTTGGCTACCCAGCAAGCACAAATCGACCAGTGTAAACAGTGGGGCGCAGAGGCCATTTTGCTCGGTAGTAGCACGACCTCATTTCCCGACCAGCAAAAGCAGGTAGCAAGCCTGCCGGTGATCg                                                                                                                            >  1:956951/1‑140 (MQ=255)             gcggcTACAGCCAGTTGGCTACCCAGCAAGCACAAATCGACCAGTGTAAACAGTGGGGCGCAGAGGCCATTTTGCTCGGTAGTAGCACGACCTCATTTCCCGACCTGCAAAAGCAGGTAGCAAGCCTGCCGGTGATCGaa                                                                                                                          <  1:290706/140‑1 (MQ=255)                  tACAGCCAGTTGGCTACCCAGCAAGCACAAATCGACCAGTGTAAACAGTGGGGCGCAGAGGCCATTTTGCTCGGTAGTAGCACGACCTCATTTCCCGACCTGCAAAAGCAGGTAGCAAGCCTGCCGGTGATCGAACTGGt                                                                                                                     <  1:811943/140‑1 (MQ=255)                  tACAGCCAGTTGGCTACCCAGCAAGCACAAATCGACCAGTGTAAACAGTGGGGCGCAGAGGCCATTTTGCTCGGTAGTAGCACGACCTCATTTCCCGACCTGCAAAAGCAGGTAGCAAGCCTGCCGGTGATCGAACTGGt                                                                                                                     <  1:1408424/140‑1 (MQ=255)                   aCAGCCAGTTGGCTACCCAGCAAGCACAAATCGACCAGTGTAAACAGTGGGGCGCAGAGGCCATTTTGCTCGGTAGTAGCACGACCTCATTTCCCGACCTGCAAAAGCAGGTAGCAAGCCTGCCGGTGATCGAACTGGTa                                                                                                                    <  2:804553/140‑1 (MQ=255)                       ccAGTTGGCTACCCAGCAAGCACAAATCGACCAGTGTAAACAGTGGGGCGCAGAGGCCATTTTGCTCGGTAGTAGCACGACCTCATTTCCCGACCTGCAAAAGCAGGTAGCAAGCCTGCCGGTGATCGAACTGGTAAATg                                                                                                                >  2:1113918/1‑140 (MQ=255)                           ttGGCTACCCAGCAAGCACAAATCGACCAGTGTAAACAGTGGGGCGCAGAGGCCATTTTGCTCGGTAGTAGCACGACCTCATTTCCCGACCTGCAAAAGCAGGTAGCAAGCCTGCCGGTGATCGAACTGGTAAATGCTAt                                                                                                            <  2:1065435/140‑1 (MQ=255)                           ttGGCTACCCAGCAAGCACAAATCGACCAGTGTAAACAGTGGGGCGCAGAGGCCATTTTGCTCGGTAGTAGCACGACCTCATTTCCCGACCTGCAAAAGCAGGTAGCAAGCCTGCCGGTGATCGAACTGGTAAATGCTAt                                                                                                            <  1:371277/140‑1 (MQ=255)                           ttGGCTACCCAGCAAGCACAAATCGACCAGTGTAAACAGTGGGGCGCAGAGGCCATTTTGCTCGGTAGTAGCACGACCTCATTTCCCGACCTGCAAAAGCAGGTAGCAAGCCTGCCGGTGATCGAACTGGTAAATGCTAt                                                                                                            >  1:53967/1‑140 (MQ=255)                           ttGGCTACCCAGCAAGCACAAATCGACCAGTGTAAACAGTGGGGCGCAGAGGCCATTTTGCTCGGTAGTAGCACGACCTCATTTCCCGACCTGCAAAAGCAGGTAGCAAGCCTGCCGGTGATCGAACTGGTAAATGCTAt                                                                                                            >  2:751413/1‑140 (MQ=255)                              gCTACCCAGCAAGCACAAATCGACCAGTGTAAACAGTGGGGCGCAGAGGCCATTTTGCTCGGTAGTAGCACGACCTCATTTCCCGACCTGCAAAAGCAGGTAGCAAGCCTGCCGGTGATCGAACTGGTAAATGCTATTGa                                                                                                         >  1:59658/1‑140 (MQ=255)                                    cAGCAAGCACAAATCGACCAGTGTAAACAGTGGGGCGCAGAGGCCATTTTGCTCGGTAGTAGCACGACCTCATTTCCCGACCTGCAAAAGCAGGTAGCAAGCCTGCCGGTGATCGAACTGGTAAATGCTATTGATGCTcc                                                                                                   <  2:747091/140‑1 (MQ=255)                                      gcaagcaCAAATCGACCAGTGTAAACAGTGGGGCGCAGAGGCCATTTTGCTCGGTAGTAGCACGACCTCATTTCCCGACCTGCAAAAGCAGGTAGCAAGCCTGCCGGTGATCGAACTGGTAAATGCTATTGATGCTcccc                                                                                                 <  2:59658/140‑1 (MQ=255)                                      gcaagcaCAAATCGACCAGTGTAAACAGTGGGGCGCAGAGGCCATTTTGCTCGGTAGTAGCACGACCTCATTTCCCGACCTGCAAAAGCAGGTAGCAAGCCTGCCGGTGATCGAACTGGTAAATGCTATTGATGCTcccc                                                                                                 <  2:108009/140‑1 (MQ=255)                                                     ccAGTGTAAACAGTGGGGCGCAGAGGCCATTTTGCTCGGTAGTAGCACGACCTCATTTCCCGACCTGCAAAAGCAGGTAGCAAGCCTGCCGGTGATCGAACTGGTAAATGCTATTGATGCTCCCCAGGTGAAAAGCcgcg                                                                                  <  1:700837/140‑1 (MQ=255)                                                          gtAAACAGTGGGGCGCAGAGGCCATTTTGCTCGGTAGTAGCACGACCTCATTTCCCGACCTGCAAAAGCAGGTAGCAAGCCTGCCGGTGATCGAACTGGTAAATGCTATTGATGCTCCCCAGGTGAAAAGCCGCGTTGgt                                                                             <  2:1297679/140‑1 (MQ=255)                                                           gaatcAGTGGGGCTGAGAGGACATTTTGCTGGGTAGTAGCGCGACCTCATTTCCCGTCCTGCAAAAGCAGGTAGCAAGCGTGCCGGTGATCGAACTGGTAAATGCTATTGATGCTCCCCAGGTGAAAAGCCGCGTTGgtg                                                                            <  2:733527/136‑1 (MQ=255)                                                               gaGTGCGGCGCAGAGGCCATCTTGTTCAGTAGTAGCCCGCGCTCATTTCCCGACCTGCAAAAGCAGGTAGAAAGCCTGCCGGTGATAGAACTGGTAAATGCTATTGATGCGCCCCAGGTGAAAAGCCGCGTTGGtgtgac                                                                        <  2:1148677/139‑3 (MQ=255)                                                               cAGTGGGGCGCAGAGGCCATTTTGCTCGGTAGTAGCACGACCTCATTTCCCGACCTGCAAAAGCAGGTAGCAAGCCTGCCGGTGATCGAACTGGTAAATGCTATTGATGCTCCCCAGGTGAAAAGCCGCGTTGGTGTGcc                                                                        <  2:376986/140‑1 (MQ=255)                                                               cAGTGGGGCGCAGAGGCCATTTTGCTCGGTAGTAGCACGACCTCATTTCCCGACCTGCAAAAGCAGGTAGCAAGCCTGCCGGTGATCGAACTGGTAAATGCTATTGATGCTCCCCAGGTGAAAAGCCGCGTTGGTGTGcc                                                                        <  2:944592/140‑1 (MQ=255)                                                                  tGGGGCGCAGAGGCCATTTTGCTCGGTAGTAGCACGACCTCATTTCCCGACCTGCAAAAGCAGGTAGCAAGCCTGCCGGTGATCGAACTGGTAAATGCTATTGATGCTCCCCAGGTGAAAAGCCGCGTTGGTGTGccc                                                                       <  2:1103744/138‑1 (MQ=255)                                                                  tGGGGCGCAGAGGCCATTTTGCTCGGTAGTAGCACGACCTCATTTCCCGACCTGCAAAAGCAGGTAGCAAGCCTGCCGGTGATCGAACTGGTAAATGCTATTGATGCTCCCCAGGTGAAAAGCCGCGTTGGTGTGccc                                                                       >  1:1103744/1‑138 (MQ=255)                                                                  tGGGGCGCAGAGGCCATTTTGCTCGGTAGTAGCACGACCTCATTTCCCGACCTGCAAAAGCAGGTAGCAAGCCTGCCGGTGATCGAACTGGTAAATGCTATTGATGCTCCCCAGGTGAAAAGCCGCGTTGGTGTGCCCTg                                                                     >  2:646208/1‑140 (MQ=255)                                                                   ggggCGCAGAGGCCATTTTGCTCGGTAGTAGCTCGACCTGATTTCCCGAGCTACAAAAGCAGTTAGCAAGCCTGCCGGGGTGCGAGCTGGTAAATGCTATTGATTCTCCCCAGGGGAAGAGCCGCGCGGCTGTGCCCTgg                                                                    >  2:939119/1‑140 (MQ=255)                                                                   ggggCGCAGAGGCCATTTTGCTCGGTAGTAGCACGACCTCATTTCCCGACCTGCAAAAGCAGGTAGCAAGCCTGCCGGTGATCGAACTGGTAAATGCTATTGATGCTCCCCAGGTGAAAAGCCGCGTTGGTGTGCCCTgg                                                                    >  1:634701/1‑140 (MQ=255)                                                                   ggggCGCAGAGGCCATTTTGCTCGGTAGTAGCACGACCTCATTTCCCGACCTGCAAAAGCAGGTAGCAAGCCTGCCGGTGATCGAACTGGTAAATGCTATTGATGCTCCCCAGGTGAAAAGCCGCGTTGGTGTGCCCTgg                                                                    >  1:237373/1‑140 (MQ=255)                                                                   ggggCGCAGAGGCCATTTTGCTCGGTAGTAGCACGACCTCATTTCCCGACCTGCAAAAGCAGGTAGCAAGCCTGCCGGTGATCGAACTGGTAAATGCTATTGATGCTCCCCAGGTGAAAAGCCGCGTTGGTGTGCCCTgg                                                                    >  2:133422/1‑140 (MQ=255)                                                                   ggggCGCAGAGGCCATTTTGCTCGGTAGTAGCACGACCTCATTTCCCGACCTGCAAAAGCAGGTAGCAAGCCTGCCGGTGATCGAACTGGTAAATGCTATTGATGCTCCCCAGGTGAAAAGCCGCGTTGGTGTGCCCTgg                                                                    >  1:659504/1‑140 (MQ=255)                                                                   ggggCGCAGAGGCCATTTTGCTCGGTAGTAGCACGACCTCATTTCCCGACCTGCAAAAGCAGGTAGCAAGCCTGCCGGTGATCGAACTGGTAAATGCTATTGATGCTCCCCAGGTGAAAAGCCGCGTTGGTGTGCCCTgg                                                                    >  2:298193/1‑140 (MQ=255)                                                                   ggggCGCAGAGGCCATTTTGCTCGGTAGTAGCACGACCTCATTTCCCGACCTGCAAAAGCAGGTAGCAAGCCTGCCGGTGATCGAACTGGTAAATGCTATTGATGCTCCCCAGGTGAAAAGCCGCGTTGGTGTGCCCTgg                                                                    >  1:165327/1‑140 (MQ=255)                                                                   ggggCGCAGAGGCCATTTTGCTCGGTAGTAGCACGACCTCATTTCCCGACCTGCAAAAGCAGGTAGCAAGCCTGCCGGTGATCGAACTGGTAAATGCTATTGATGCTCCCCAGGTGAAAAGCCGCGTTGGTGTGCCCTgg                                                                    >  1:837235/1‑140 (MQ=255)                                                                   ggggCGCAGAGGCCATTTTGCTCGGTAGTAGCACGACCTCATTTCCCGACCTGCAAAAGCAGGTAGCAAGCCTGCCGGTGATCGAACTGGTAAATGCTATTGATGCTCCCCAGGTGAAAAGCCGCGTTGGTGTGCCCTgg                                                                    >  1:141063/1‑140 (MQ=255)                                                                    gggCGCAGAGGCCATTTTGCTCGGTAGTAGCACGACCTCATTTCCCGACCTGCAAAAGCAGGTAGCAAGCCTGCCGGTGATCGAACTGGTAAATGCTATTGATGCTCCCCAGGTGAAAAGCCGCGTTGGTGTGCCCTGGt                                                                   >  2:793986/1‑140 (MQ=255)                                                                         cAGAGGCCATTTTGCTCGGTAGTAGCACGACCTCATTTCCCGACCTGCAAAAGCAGGTAGCAAGCCTGCCGGTGATCGAACTGGTAAATGCTATTGATGCTCCCCAGGTGAAAAGCCGCGTTGGTGTGCCCTGGTTTCAg                                                              <  1:793986/140‑1 (MQ=255)                                                                           gagGCCATTTTGCTCGGTAGTAGCACGACCTCATTTCCCGACCTGCAAAAGCAGGTAGCAAGCCTGCCGGTGATCGAACTGGTAAATGCTATTGATGCTCCCCAGGTGAAAAGCCGCGTTGGTGTGCCCTGGTTTCAGAt                                                            <  2:892447/140‑1 (MQ=255)                                                                           gagGCCATTTTGCTCGGTAGTAGCACGACCTCATTTCCCGACCTGCAAAAGCAGGTAGCAAGCCTGCCGGTGATCGAACTGGTAAATGCTATTGATGCTCCCCAGGTGAAAAGCCGCGTTGGTGTGCCCTGGTTTCAGAt                                                            <  1:1113918/140‑1 (MQ=255)                                                                                      gCTCGGTAGTAGCACGACCTCATTTCCCGACCTGCAAAAGCAGGTAGCAAGCCTGCCGGTGATCGAACTGGTAAATGCTATTGATGCTCCCCAGGTGAAAAGCCGCGTTGGTGTGCCCTGGTTTCAGATGGGCTATCAAc                                                 <  2:53967/140‑1 (MQ=255)                                                                                        tCGGTAGTAGCACGACCTCATTTCCCGACCTGCAAAAGCAGGTAGCAAGCCTGCCGGTGATCGAACTGGTAAATGCTATTGATGCTCCCCAGGTGAAAAGCCGCGTTGGTGTGCCCTGGTTTCAGATGGGCTATCAACCg                                               >  1:1099729/1‑140 (MQ=255)                                                                                             agtagCACGACCTCATTTCCCGACCTGCAAAAGCAGGTAGCAAGCCTGCCGGTGATCGAACTGGTAAATGCTATTGATGCTCCCCAGGTGAAAAGCCGCGTTGGTGTGCCCTGGTTTCAGATGGGCTATCAACCGGGGCg                                          <  2:1166015/140‑1 (MQ=255)                                                                                               tagCACGACCTCATTTCCCGACCTGCAAAAGCAGGTAGCAAGCCTGCCGGTGATCGAACTGGTAAATGCTATTGATGCTCCCCAGGTGAAAAGCCGCGTTGGTGTGCCCTGGTTTCAGATGGGCTATCAACCGGGGCGat                                        >  1:1099956/1‑140 (MQ=255)                                                                                               tagCACGACCTCATTTCCCGACCTGCAAAAGCAGGTAGCAAGCCTGCCGGTGATCGAACTGGTAAATGCTATTGATGCTCCCCAGGTGAAAAGCCGCGTTGGTGTGCCCTGGTTTCAGATGGGCTATCAACCGGGGCGat                                        <  1:748125/140‑1 (MQ=255)                                                                                               tagCACGACCTCATTTCCCGACCTGCAAAAGCAGGTAGCAAGCCTGCCGGTGATCGAACTGGTAAATGCTATTGATGCTCCCCAGGTGAAAAGCCGCGTTGGTGTGCCCTGGTTTCAGATGGGCTATCAACCGGGGCGat                                        <  1:763070/140‑1 (MQ=255)                                                                                                    cGACCTCATTTCCCGACCTGCAAAAGCAGGTAGCAAGCCTGCCGGTGATCGAACTGGTAAATGCTATTGATGCTCCCCAGGTGAAAAGCCGCGTTGGTGTGCCCTGGTTTCAGATGGGCTATCAACCGGGGCGATATCTg                                   >  1:711696/1‑140 (MQ=255)                                                                                                      aCCTCATTTCCCGACCTGCAAAAGCAGGTAGCAAGCCTGCCGGTGATCGAACTGGTAAATGCTATTGATGCTCCCCAGGTGAAAAGCCGCGTTGGTGTGCCCTGGTTTCAGATGGGCTATCAACCGGGGCGATATCTggg                                 >  2:283867/1‑139 (MQ=255)                                                                                                      aCCTCATTTCCCGACCTGCAAAAGCAGGTAGCAAGCCTGCCGGTGATCGAACTGGTAAATGCTATTGATGCTCCCCAGGTGAAAAGCCGCGTTGGTGTGCCCTGGTTTCAGATGGGCTATCAACCGGGGCGATATCTGGt                                 >  1:1027092/1‑140 (MQ=255)                                                                                                      aCCTCATTTCCCGACCTGCAAAAGCAGGTAGCAAGCCTGCCGGTGATCGAACTGGTAAATGCTATTGATGCTCCCCAGGTGAAAAGCCGCGTTGGTGTGCCCTGGTTTCAGATGGGCTATCAACCGGGGCGATATCTGGt                                 >  2:682835/1‑140 (MQ=255)                                                                                                      aCCTCATTTCCCGACCTGCAAAAGCAGGTAGCAAGCCTGCCGGTGATCGAACTGGTAAATGCTATTGATGCTCCCCAGGTGAAAAGCCGCGTTGGTGTGCCCTGGTTTCAGATGGGCTATCAACCGGGGCGATATCTGGt                                 >  1:848767/1‑140 (MQ=255)                                                                                                      aCCTCATTTCCCGACCTGCAAAAGCAGGTAGCAAGCCTGCCGGTGATCGAACTGGTAAATGCTATTGATGCTCCCCAGGTGAAAAGCCGCGTTGGTGTGCCCTGGTTTCAGATGGGCTATCAACCGGGGCGATATCTGGt                                 >  2:901519/1‑140 (MQ=255)                                                                                                      aCCTCATTTCCCGACCTGCAAAAGCAGGTAGCAAGCCTGCCGGTGATCGAACTGGTAAATGCTATTGATGCTCCCCAGGTGAAAAGCCGCGTTGGTGTGCCCTGGTTTCAGATGGGCTATCAACCGGGGCGATATCTGGt                                 >  2:134441/1‑140 (MQ=255)                                                                                                      aCCTCATTTCCCGACCTGCAAAAGCAGGTAGCAAGCCTGCCGGTGATCGAACTGGTAAATGCTATTGATGCTCCCCAGGTGAAAAGCCGCGTTGGTGTGCCCTGGTTTCAGATGGGCTATCAACCGGGGCGATATCTGGt                                 >  2:152058/1‑140 (MQ=255)                                                                                                       ccTCATTTCCCGACCTGCAAAAGCAGGTAGCAAGCCTGCCGGTGATCGAACTGGTAAATGCTATTGATGCTCCCCAGGTGAAAAGCCGCGTTGGTGTGCCCTGGTTTCAGATGGGCTATCAACCGGGGCGATATCTGGTg                                <  2:1099729/140‑1 (MQ=255)                                                                                                         tCATTTCCCGACCTGCAAAAGCAGGTAGCAAGCCTGCCGGTGATCGAACTGGTAAATGCTATTGATGCTCCCCAGGTGAAAAGCCGCGTTGGTGTGCCCTGGTTTCAGATGGGCTATCAACCGGGGCGATATCTGGTGCa                              >  2:100524/1‑140 (MQ=255)                                                                                                           aTTTCCCGACCTGCAAAAGCAGGTAGCAAGCCTGCCGGTGATCGAACTGGTAAATGCTATTGATGCTCCCCAGGTGAAAAGCCGCGTTGGTGTGCCCTGGTTTCAGATGGGCTATCAACCGGGGCGATATCTGGTGCAAt                            <  1:751413/140‑1 (MQ=255)                                                                                                           aTTTCCCGACCTGCAAAAGCAGGTAGCAAGCCTGCCGGTGATCGAACTGGTAAATGCTATTGATGCTCCCCAGGTGAAAAGCCGCGTTGGTGTGCCCTGGTTTCAGATGGGCTATCAACCGGGGCGATATCTGGTGCAAt                            <  2:141063/140‑1 (MQ=255)                                                                                                              tCCCGACCTGCAAAAGCAGGTAGCAAGCCTGCCGGTGATCGAACTGGTAAATGCTATTGATGCTCCCCAGGTGAAAAGCCGCGTTGGTGTGCCCTGGTTTCAGATGGGCTATCAACCGGGGCGATATCTGGTGCAATggg                         <  2:251385/140‑1 (MQ=255)                                                                                                                ccGACCTGCAAAAGCAGGTAGCAAGCCTGCCGGTGATCGAACTGGTAAATGCTATTGATGCTCCCCAGGTGAAAAGCCGCGTTGGTGTGCCCTGGTTTCAGATGGGCTATCAACCGGGGCGATATCTGGTGCAATGGgcg                       <  1:748522/140‑1 (MQ=255)                                                                                                                ccGACCTGCAAAAGCAGGTAGCAAGCCTGCCGGTGATCGAACTGGTAAATGCTATTGATGCTCCCCAGGTGAAAAGCCGCGTTGGTGTGCCCTGGTTTCAGATGGGCTATCAACCGGGGCGATATCTGGTGCAATGGgcg                       <  2:659504/140‑1 (MQ=255)                                                                                                                ccGACCTGCAAAAGCAGGTAGCAAGCCTGCCGGTGATCGAACTGGTAAATGCTATTGATGCTCCCCAGGTGAAAAGCCGCGTTGGTGTGCCCTGGTTTCAGATGGGCTATCAACCGGGGCGATATCTGGTGCAATGGgcg                       <  2:848767/140‑1 (MQ=255)                                                                                                                 cGACCTGCAAAAGCAGGTAGCAAGCCTGCCGGTGATCGAACTGGTAAATGCTATTGATGCTCCCCAGGTGAAAAGCCGCGTTGGTGTGCCCTGGTTTCAGATGGGCTATCAACCGGGGCGATATCTGGTGCAATGGgcgc                      >  1:348156/1‑140 (MQ=255)                                                                                                                 cGACCTGCAAAAGCAGGTAGCAAGCCTGCCGGTGATCGAACTGGTAAATGCTATTGATGCTCCCCAGGTGAAAAGCCGCGTTGGTGTGCCCTGGTTTCAGATGGGCTATCAACCGGGGCGATATCTGGTGCAATGGgcgc                      >  1:334176/1‑140 (MQ=255)                                                                                                                 cGACCTGCAAAAGCAGGTAGCAAGCCTGCCGGTGATCGAACTGGTAAATGCTATTGATGCTCCCCAGGTGAAAAGCCGCGTTGGTGTGCCCTGGTTTCAGATGGGCTATCAACCGGGGCGATATCTGGTGCAATGGgcgc                      >  2:572554/1‑140 (MQ=255)                                                                                                                 cGACCTGCAAAAGCAGGTAGCAAGCCTGCCGGTGATCGAACTGGTAAATGCTATTGATGCTCCCCAGGTGAAAAGCCGCGTTGGTGTGCCCTGGTTTCAGATGGGCTATCAACCGGGGCGATATCTGGTGCAATGGgcgc                      >  1:546478/1‑140 (MQ=255)                                                                                                                  gACCTGCAAAAGCAGGTAGCAAGCCTGCCGGTGATCGAACTGGTAAATGCTATTGATGCTCCCCAGGTGAAAAGCCGCGTTGGTGTGCCCTGGTTTCAGATGGGCTATCAACCGGGGCGATATCTGGTGCAATGGGCGCa                     <  1:901519/140‑1 (MQ=255)                                                                                                                  gACCTGCAAAAGCAGGTAGCAAGCCTGCCGGTGATCGAACTGGTAAATGCTATTGATGCTCCCCAGGTGAAAAGCCGCGTTGGTGTGCCCTGGTTTCAGATGGGCTATCAACCGGGGCGATATCTGGTGCAATGGGCGCa                     <  2:693041/140‑1 (MQ=255)                                                                                                                  gACCTGCAAAAGCAGGTAGCAAGCCTGCCGGTGATCGAACTGGTAAATGCTATTGATGCTCCCCAGGTGAAAAGCCGCGTTAGTGTGCCCTGGTTTCAGATGGGCTATCAACCGGGGCGATATCTGGTGCAATGGGCGCa                     >  2:1244770/1‑140 (MQ=255)                                                                                                                   aCCTGCAAAAGCAGGTAGCAAGCCTGCCGGTGATCGAACTGGTAAATGCTATTGATGCTCCCCAGGTGAAAAGCCGCGTTGGTGTGCCCTGGTTTCAGATGGGCTATCAACCGGGGCGATATCTGGTGCAATGGGCGCAc                    >  2:774921/1‑140 (MQ=255)                                                                                                                    ccTGCAAAAGCAGGTAGCAAGCCTGCCGGTGATCGAACTGGTAAATGCTATTGATGCTCCCCAGGTGAAAAGCCGCGTTGGTGTGCCCTGGTTTCAGATGGGCTATCAACCGGGGCGATATCTGGTGCAATGGGCGCACg                   <  2:348156/140‑1 (MQ=255)                                                                                                                     cTGCAAAAGCAGGTAGCAAGCCTGCCGGTGATCGAACTGGTAAATGCTATTGATGCTCCCCAGGTGAAAAGCCGCGTTGGTGTGCCCTGGTTTCAGATGGGCTATCAACCGGGGCGATATCTGGTGCAATGGGCGCACgg                  >  2:120330/1‑140 (MQ=255)                                                                                                                     cTGCAAAAGCAGGTAGCAAGCCTGCCGGTGATCGAACTGGTAAATGCTATTGATGCTCCCCAGGTGAAAAGCCGCGTTGGTGTGCCCTGGTTTCAGATGGGCTATCAACCGGGGCGATATCTGGTGCAATGGGCGCACgg                  >  2:1071060/1‑140 (MQ=255)                                                                                                                      tGCAAAAGCAGGTAGCAAGCCTGCCGGTGATCGAACTGGTAAATGCTATTGATGCTCCCCAGGTGAAAAGCCGCGTTGGTGTGCCCTGGTTTCAGATGGGCTATCAACCGGGGCGATATCTGGTGCAATGGGCGCACGGt                 >  2:199186/1‑140 (MQ=255)                                                                                                                       gCAAAAGCAGGTAGCAAGCCTGCCGGTGATCGAACTGGTAAATGCTATTGATGCTCCCCAGGTGAAAAGCCGCGTTGGTGTGCCCTGGTTTCAGATGGGCTATCAACCGGGGCGATATCTGGTGCAATGGGCGCACGGTa                <  1:572554/140‑1 (MQ=255)                                                                                                                       gCAAAAGCAGGTAGCAAGCCTGCCGGTGATCGAACTGGTAAATGCTATTGATGCTCCCCAGGTGAAAAGCCGCGTTGGTGTGCCCTGGTTTCAGATGGGCTATCAACCGGGGCGATATCTGGTGCAATGGGCGCACGGTa                >  1:190034/1‑140 (MQ=255)                                                                                                                       gCAAAAGCAGGTAGCAAGCCTGCCGGTGATCGAACTGGTAAATGCTATTGATGCTCCCCAGGTGAAAAGCCGCGTTGGTGTGCCCTGGTTTCAGATGGGCTATCAACCGGGGCGATATCTGGTGCAATGGGCGCACGGTa                <  2:797348/140‑1 (MQ=255)                                                                                                                       gCAAAAGCAGGTAGCAAGCCTGCCGGTGATCGAACTGGTAAATGCTATTGATGCTCCCCAGGTGAAAAGCCGCGTTGGTGTGCCCTGGTTTCAGATGGGCTATCAACCGGGGCGATATCTGGTGCAATGGGCGCACGGTa                >  1:989/1‑140 (MQ=255)                                                                                                                        cAAAAGCAGGTAGCAAGCCTGCCGGTGATCGAACTGGTAAATGCTATTGATGCTCCCCAGGTGAAAAGCCGCGTTGGTGTGCCCTGGTTTCAGATGGGCTATCAACCGGGGCGATATCTGGTGCAATGGGCGCACGGTaa               <  2:634701/140‑1 (MQ=255)                                                                                                                        cAAAAGCAGGTAGCAAGCCTGCCGGTGATCGAACTGGTAAATGCTATTGATGCTCCCCAGGTGAAAAGCCGCGTTGGTGTGCCCTGGTTTCAGATGGGCTATCAACCGGGGCGATATCTGGTGCAATGGGCGCACGGTaa               >  2:1335735/1‑140 (MQ=255)                                                                                                                           aaGCAGGTAGCAAGCCTGCCGGTGATCGAACTGGTAAATGCTATTGATGCTCCCCAGGTGAAAAGCCGCGTTGGTGTGCCCTGGTTTCAGATGGGCTATCAACCGGGGCGATATCTGGTGCAATGGGCGCACGGTAAAcc            <  2:190034/140‑1 (MQ=255)                                                                                                                              cAGGTAGCAAGCCTGCCGGTGATCGAACTGGTAAATGCTATTGATGCTCCCCAGGTGAAAAGCCGCGTTGGTGTGCCCTGGTTTCAGATGGGCTATCAACCGGGGCGATATCTGGTGCAATGGGCGCACGGTAAACCACt         <  2:546478/140‑1 (MQ=255)                                                                                                                              cAGGTAGCAAGCCTGCCGGTGATCGAACTGGTAAATGCTATTGATGCTCCCCAGGTGAAAAGCCGCGTTGGTGTGCCCTGGTTTCAGATGGGCTATCAACCGGGGCGATATCTGGTGCAATGGGCGCACGGTAAACCACt         <  2:334176/140‑1 (MQ=255)                                                                                                                              cAGGTAGCAAGCCTGCCGGTGATCGAACTGGTAAATGCTATTGATGCTCCCCAGGTGAAAAGCCGCGTTGGTGTGCCCTGGTTTCAGATGGGCTATCAACCGGGGCGATATCTGGTGCAATGGGCGCACGGTAAACCACt         <  1:1071060/140‑1 (MQ=255)                                                                                                                                 gTAGCAAGCCTGCCGGTGATCGAACTGGTAAATGCTATTGATGCTCCCCAGGTGAAAAGCCGCGTTGGTGTGCCCTGGTTTCAGATGGGCTATCAACCGGGGCGATATCTGGTGCAATGGGCGCACGGTAAACCACTGaa      >  1:515197/1‑140 (MQ=255)                                                                                                                                     cAAGCCTGCCGGTGATCGAACTGGTAAATGCTATTGATGCTCCCCAGGTGAAAAGCCGCGTTGGTGTGCCCTGGTTTCAGATGGGCTATCAACCGGGGCGATATCTGGTGCAATGGGCGCACGGTAAACCACTGAAtgtg  <  2:837235/140‑1 (MQ=255)                                                                                                                                     cAAGCCTGCCGGTGATCGAACTGGTAAATGCTATTGATGCTCCCCAGGTGAAAAGCCGCGTTGGTGTGCCCTGGTTTCAGATGGGCTATCAACCGGGGCGATATCTGGTGCAATGGGCGCACGGTAAACCACTGAAtgtg  <  1:152058/140‑1 (MQ=255)                                                                                                                                         |                                                                                                                                        TGCTGGAGGCAGGCGGCTACAGCCAGTTGGCTACCCAGCAAGCACAAATCGACCAGTGTAAACAGTGGGGCGCAGAGGCCATTTTGCTCGGTAGTAGCACGACCTCATTTCCCGACCTGCAAAAGCAGGTAGCAAGTCTGCCGGTGATCGAACTGGTAAATGCTATTGATGCTCCCCAGGTGAAAAGCCGCGTTGGTGTGCCCTGGTTTCAGATGGGCTATCAACCGGGGCGATATCTGGTGCAATGGGCGCACGGTAAACCACTGAATGTG  >  NC_000913/1056496‑1056767

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑