Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,283,138	C→T	P1092L (CCG→CTG)	narG →	nitrate reductase A subunit alpha

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,283,138	0	C	T	100.0%	210.6 / NA	59	P1092L (CCG→CTG)	narG	nitrate reductase A subunit alpha
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (23/36);  total (23/36)

AGGATCACCAGTGGATGCGTGATTTCGGTGAAAGCCTGCTGGTTTATCGTCCGCCGATCGACACCCGTTCGGTGAAAGAAGTGATAGGCCAGAAATCCAACGGCAACCAGGAAAAAGCGCTCAACTTCCTGACGCCGCACCAGAAGTGGGGTATCCACTCCACCTACAGCGACAACCTGCTGATGCTGACTTTAGGTCGCGGTGGTCCGGTGGTCTGGTTGAGTGAAGCCGATGCCAAAGATCTGGGTATCGCCGATAACGACTGGATTGAA  >  NC_000913/1283003‑1283274                                                                                                                                        |                                                                                                                                         aGGATCACCAGTGGATGCGTGATTTCGGTGAAAGCCTGCTGGTTTATCGTCCGCCGATCGACACCCGTTCGGTGAAAGAAGTGATAGGCCAGAAATCCAACGGCAACCAGGAAAAAGCGCTCAACTTCCCGACGCTGCAc                                                                                                                                      <  1:838552/140‑1 (MQ=255)    aTCACCAGTGGATGCGTGATTTCGGTGAAAGCCTGCTGGTTTATCGTCCGCCGATCGACACCCGTTCGGTGAAAGAAGTGATAGGCCAGAAATCCAACGGCAACCAGGAAAAAGCGCTCAACTTCCTGACGCTGCACCAg                                                                                                                                   <  1:209206/140‑1 (MQ=255)    aTCACCAGTGGATGCGTGATTTCGGTGAAAGCCTGCTGGTTTATCGTCCGCCGATCGACACCCGTTCGGTGAAAGAAGTGATAGGCCAGAAATCCAACGGCAACCAGGAAAAAGCGCTCAACTTCCTGACGCTGCACCAg                                                                                                                                   >  2:1418820/1‑140 (MQ=255)     tCACCAGTGGATGCGTGATTTCGGTGAAAGCCTGCTGGTTTATCGTCCGCCGATCGACACCCGTTCGGTGAAAGAAGTGATAGGCCAGAAATCCAACGGCAACCAGGAAAAAGCGCTCAACTTCCTGACGCTGCACCAGa                                                                                                                                  >  2:276901/1‑140 (MQ=255)     tCACCAGTGGATGCGTGATTTCGGTGAAAGCCTGCTGGTTTATCGTCCGCCGATCGACACCCGTTCGGTGAAAGAAGTGATAGGCCAGAAATCCAACGGCAACCAGGAAAAAGCGCTCAACTTCCTGACGCTGCACCAGa                                                                                                                                  >  2:1200836/1‑140 (MQ=255)      cACCAGTGGATGCGTGATTTCGGTGAAAGCCTGCTGGTTTATCGTCCGCCGATCGACACCCGTTCGGTGAAAGAAGTGATAGGCCAGAAATCCAACGGCAACCAGGAAAAAGCGCTCAACTTCCTGACGCTGCACCAGaa                                                                                                                                 <  1:636530/140‑1 (MQ=255)       aCCAGTGGATGCGTGATTTCGGTGAAAGCCTGCTGGTTTATCGTCCGCCGATCGACACCCGTTCGGTGAAAGAAGTGATAGGCCAGAAATCCAACGGCAACCAGGAAAAAGCGCTCAACTTCCTGACGCTGCACCAGAAg                                                                                                                                >  1:914826/1‑140 (MQ=255)       aCCAGTGGATGCGTGATTTCGGTGAAAGCCTGCTGGTTTATCGTCCGCCGATCGACACCCGTTCGGTGAAAGAAGTGATAGGCCAGAAATCCAACGGCAACCAGGAAAAAGCGCTCAACTTCCTGACGCTGCACCAGAAg                                                                                                                                >  1:388122/1‑140 (MQ=255)       aCCAGTGGATGCGTGATTTCGGTGAAAGCCTGCTGGTTTATCGTCCGCCGATCGACACCCGTTCGGTGAAAGAAGTGATAGGCCAGAAATCCAACGGCAACCAGGAAAAAGCGCTCAACTTCCTGACGCTGCACCAGAAg                                                                                                                                >  2:1108036/1‑140 (MQ=255)       aCCAGTGGATGCGTGATTTCGGTGAAAGCCTGCTGGTTTATCGTCCGCCGATCGACACCCGTTCGGTGAAAGAAGTGATAGGCCAGAAATCCAACGGCAACCAGGAAAAAGCGCTCAACTTCCTGACGCTGCACCAGAAg                                                                                                                                >  2:1116399/1‑140 (MQ=255)       aCCAGTGGATGCGTGATTTCGGTGAAAGCCTGCTGGTTTATCGTCCGCCGATCGACACCCGTTCGGTGAAAGAAGTGATAGGCCAGAAATCCAACGGCAACCAGGAAAAAGCGCTCAACTTCCTGACGCTGCACCAGAAg                                                                                                                                >  2:1367266/1‑140 (MQ=255)       aCCAGTGGATGCGTGATTTCGGTGAAAGCCTGCTGGTTTATCGTCCGCCGATCGACACCCGTTCGGTGAAAGAAGTGATAGGCCAGAAATCCAACGGCAACCAGGAAAAAGCGCTCAACTTCCTGACGCTGCACCAGAAg                                                                                                                                >  2:250080/1‑140 (MQ=255)       aCCAGTGGATGCGTGATTTCGGTGAAAGCCTGCTGGTTTATCGTCCGCCGATCGACACCCGTTCGGTGAAAGAAGTGATAGGCCAGAAATCCAACGGCAACCAGGAAAAAGCGCTCAACTTCCTGACGCTGCACCAGAAg                                                                                                                                >  1:1225964/1‑140 (MQ=255)       aCCAGTGGATGCGTGATTTCGGTGAAAGCCTGCTGGTTTATCGTCCGCCGATCGACACCCGTTCGGTGAAAGAAGTGATAGGCCAGAAATCCAACGGCAACCAGGAAAAAGCGCTCAACTTCCTGACGCTGCACCAGAAg                                                                                                                                >  2:440546/1‑140 (MQ=255)        ccAGTGGATGCGTGATTTCGGTGAAAGCCTGCTGGTTTATCGTCCGCCGATCGACACCCGTTCGGTGAAAGAAGTGATAGGCCAGAAATCCAACGGCAACCAGGAAAAAGCGCTCAACTTCCTGACGCTGCACCAGAAGt                                                                                                                               <  1:590251/140‑1 (MQ=255)        ccAGTGGATGCGTGATTTCGGTGAAAGCCTGCTGGTTTATCGTCCGCCGATCGACACCCGTTCGGTGAAAGAAGTGATAGGCCAGAAATCCAACGGCAACCAGGAAAAAGCGCTCAACTTCCTGACGCTGCACCAGAAGt                                                                                                                               <  1:270621/140‑1 (MQ=255)        ccAGTGGATGCGTGATTTCGGTGAAAGCCTGCTGGTTTATCGTCCGCCGATCGACACCCGTTCGGTGAAAGAAGTGATAGGCCAGAAATCCAACGGCAACCAGGAAAAAGCGCTCAACTTCCTGACGCTGCACCAGAAGt                                                                                                                               <  1:1134924/140‑1 (MQ=255)        ccAGTGGATGCGTGATTTCGGTGAAAGCCTGCTGGTTTATCGTCCGCCGATCGACACCCGTTCGGTGAAAGAAGTGATAGGCCAGAAATCCAACGGCAACCAGGAAAAAGCGCTCAACTTCCTGACGCTGCACCAGAAGt                                                                                                                               <  1:427250/140‑1 (MQ=255)             ggATGCGTGATTTCGGTGAAAGCCTGCTGGTTTATCGTCCGCCGATCGACACCCGTTCGGTGAAAGAAGTGATAGGCCAGAAATCCAACGGCAACCAGGAAAAAGCGCTCAACTTCCTGACGCTGCACCAGAAGTGGGGt                                                                                                                          >  2:64114/1‑140 (MQ=255)               aTGCGTGATTTCGGTGAAAGCCTGCTGGTTTATCGTCCGCCGATCGACACCCGTTCGGTGAAAGAAGTGATAGGCCAGAAATCCAACGGCAACCAGGAAAAAGCGCTCAACTTCCTGACGCTGCACCAGAAGTGGGGTAt                                                                                                                        <  1:1101875/140‑1 (MQ=255)               aTGCGTGATTTCGGTGAAAGCCTGCTGGTTTATCGTCCGCCGATCGACACCCGTTCGGTGAAAGAAGTGATAGGCCAGAAATCCAACGGCAACCAGGAAAAAGCGCTCAACTTCCTGACGCTGCACCAGAAGTGGGGTAt                                                                                                                        <  1:440546/140‑1 (MQ=255)                 gCGTGATTTCGGTGAAAGCCTGCTGGTTTATCGTCCGCCGATCGACACCCGTTCGGTGAAAGAAGTGATAGGCCAGAAATCCAACGGCAACCAGGAAAAAGCGCTCAACTTCCTGACGCTGCACCAGAAGTGGGGTAtcc                                                                                                                      <  2:1419808/140‑1 (MQ=255)                 gCGTGATTTCGGTGAAAGCCTGCTGGTTTATCGTCCGCCGATCGACACCCGTTCGGTGAAAGAAGTGATAGGCCAGAAATCCAACGGCAACCAGGAAAAAGCGCTCAACTTCCTGACGCTGCACCAGAAGTGGGGTAtcc                                                                                                                      <  2:174413/140‑1 (MQ=255)                 gCGTGATTTCGGTGAAAGCCTGCTGGTTTATCGTCCGCCGATCGACACCCGTTCGGTGAAAGAAGTGATAGGCCAGAAATCCAACGGCAACCAGGAAAAAGCGCTCAACTTCCTGACGCTGCACCAGAAGTGGGGTAtcc                                                                                                                      >  1:64644/1‑140 (MQ=255)                   gTGATTTCGGTGAAAGCCTGCTGGTTTATCGTCCGCCGATCGACACCCGTTCGGTGAAAGAAGTGATAGGCCAGAAATCCAACGGCAACCAGGAAAAAGCGCTCAACTTCCTGACGCTGCACCAGAAGTGGGGTAtccac                                                                                                                    >  1:830685/1‑140 (MQ=255)                   gTGATTTCGGTGAAAGCCTGCTGGTTTATCGTCCGCCGATCGACACCCGTTCGGTGAAAGAAGTGATAGGCCAGAAATCCAACGGCAACCAGGAAAAAGCGCTCAACTTCCTGACGCTGCACCAGAAGTGGGGTAtccac                                                                                                                    >  1:778361/1‑140 (MQ=255)                     gATTTCGGTGAAAGCCTGCTGGTTTATCGTCCGCCGATCGACACCCGTTCGGTGAAAGAAGTGATAGGCCAGAAATCCAACGGCAACCAGGAAAAAGCGCTCAACTTCCTGACGCTGCACCAGAAGTGGGGTAtccactc                                                                                                                  <  1:609121/140‑1 (MQ=255)                           ggTGAAAGCCTGCTGGTTTATCGTCCGCCGATCGACACCCGTTCGGTGAAAGAAGTGATAGGCCAGAAATCCAACGGCAACCAGGAAAAAGCGCTCAACTTCCTGACGCTGCACCAGAAGTGGGGTATCCACTCCACCTa                                                                                                            <  2:664408/140‑1 (MQ=255)                           ggTGAAAGCCTGCTGGTTTATCGTCCGCCGATCGACACCCGTTCGGTGAAAGAAGTGATAGGCCAGAAATCCAACGGCAACCAGGAAAAAGCGCTCAACTTCCTGACGCTGCACCAGAAGTGGGGTATCCACTCCACCTa                                                                                                            <  1:1108036/140‑1 (MQ=255)                           ggTGAAAGCCTGCTGGTTTATCGTCCGCCGATCGACACCCGTTCGGTGAAAGAAGTGATAGGCCAGAAATCCAACGGCAACCAGGAAAAAGCGCTCAACTTCCTGACGCTGCACCAGAAGTGGGGTATCCACTCCACCTa                                                                                                            <  1:198894/140‑1 (MQ=255)                                 aGCCTGCTGGTTTATCGTCCGCCGATCGACACCCGTTCGGTGAAAGAAGTGATAGGCCAGAAATCCAACGGCAACCAGGAAAAAGCGCTCAACTTCCTGACGCTGCACCAGAAGTGTGGTATCCACTCCACCTACAGCGa                                                                                                      <  2:409717/140‑1 (MQ=255)                                 aGCCTGCTGGTTTATCGTCCGCCGATCGACACCCGTTCGGTGAAAGAAGTGATAGGCCAGAAATCCAACGGCAACCAGGAAAAAGCGCTCAACTTCCTGACGCTGCACCAGAAGTGGGGTATCCACTCCACCTACAGCGa                                                                                                      <  1:1367266/140‑1 (MQ=255)                                        tgGTTTATCGTCCGCCGATCGACACCCGTTCGGTGAAAGAAGTGATAGGCCAGAAATCCAACGGCAACCAGGAAAAAGCGCTCAACTTCCTGACGCTGCACCAGAAGTGGGGTATCCACTCCACCTACAGCGACAACctg                                                                                               <  2:914826/140‑1 (MQ=255)                                               tCGTCCGCCGATCGACACCCGTTCGGTGAAAGAAGTGATAGGCCAGAAATCCAACGGCAACCAGGAAAAAGCGCTCAACTTCCTGACGCTGCACCAGAAGTGGGGTATCCACTCCACCTACAGCGACAACCtgctgatgc                                                                                        <  1:1376436/140‑1 (MQ=255)                                                cGTCCGCCGATCGACACCCGTTCGGTGAAAGAAGTGATAGGCCAGAAATCCAACGGCAACCAGGAAAAAGCGCTCAACTTCCTGACGCTGCACCAGAAGTGGGGTATCCACTCCACCTACAGCGACAACCtgctgatgct                                                                                       <  1:250080/140‑1 (MQ=255)                                                    cgccgATCGACACCCGTTCGGTGAAAGAAGTGATAGGCCAGAAATCCAACGGCAACCAGGAAAAAGCGCTCAACTTCCTGACGCTGCACCAGAAGTGGGGTATCCACTCCACCTACAGCGACAACCTGCTGATGCTGACt                                                                                   <  1:64114/140‑1 (MQ=255)                                                    cgccgATCGACACCCGTTCGGTGAAAGAAGTGATAGGCCAGAAATCCAACGGCAACCAGGAAAAAGCGCTCAACTTCCTGACGCTGCACCAGAAGTGGGGTATCCACTCCACCTACAGCGACAACCTGCTGATGCTGACt                                                                                   >  2:680419/1‑140 (MQ=255)                                                       cgATCGACACCCGTTCGGTGAAAGAAGTGATAGGCCAGAAATCCAACGGCAACCAGGAAAAAGCGCTCAACTTCCTGACGCTGCACCAGAAGTGGGGTATCCACTCCACCTACAGCGACAACCTGCTGATGCTGACTTTa                                                                                <  1:1200836/140‑1 (MQ=255)                                                         aTCGACACCCGTTCGGTGAAAGAAGTGATAGGCCAGAAATCCAACGGCAACCAGGAAAAAGCGCTCAACTTCCTGACGCTGCACCAGAAGTGGGGTATCCACTCCACCTACAGCGACAACCTGCTGATGCTGACttaggt                                                                              <  2:388122/140‑5 (MQ=255)                                                            gACACCCGTTCGGTGAAAGAAGTGATAGGCCAGAAATCCAACGGCAACCAGGAAAAAGCGCTCAACTTCCTGACGCTGCACCAGAAGTGGGGTATCCACTCCACCTACAGCGACAACCTGCTGATGCTGACTTTAGGTcg                                                                           <  1:1281530/140‑1 (MQ=255)                                                              cacCCGTTCGGTGAAAGAAGTGATAGGCCAGAAATCCAACGGCAACCAGGAAAAAGCGCTCAACTTCCTGACGCTGCACCAGAAGTGGGGTATCCACTCCACCTACAGCGACAACCTGCTGATGCTGACTTTAGGTcgcg                                                                         <  1:276901/140‑1 (MQ=255)                                                                 ccGTTCGGTGAAAGAAGTGATAGGCCAGAAATCCAACGGCAACCAGGAAAAAGCGCTCAACTTCCTGACGCTGCACCAGAAGTGGGGTATCCACTCCACCTACAGCGACAACCTGCTGATGCTGACTTTAGGTCGcggtg                                                                      >  2:1344964/1‑140 (MQ=255)                                                                  cGTTCGGTGAAAGAAGTGATAGGCCAGAAATCCAACGGCAACCAGGAAAAAGCGCTCAACTTCCTGACGCTGCACCAGAAGTGGGGTATCCACTCCACCTACAGCGACAACCTGCTGATGCTGACTTTAGGTCGcggtgg                                                                     <  2:159887/140‑1 (MQ=255)                                                                    ttCGGTGAAAGAAGTGATAGGCCAGAAATCCAACGGCAACCAGGAAAAAGCGCTCAACTTCCTGACGCTGCACCAGAAGTGGGGTATCCACTCCACCTACAGCGACAACCTGCTGATGCTGACTTTAGGTCGcggtggtc                                                                   >  2:441630/1‑140 (MQ=255)                                                                       ggTGAAAGAAGTGATAGGCCAGAAATCCAACGGCAACCAGGAAAAAGCGCTCAACTTCCTGACGCTGCACCAGAAGTGGGGTATCCACTCCACCTACAGCGACAACCTGCTGATGCTGACTTTAGGTCGcggtggtccgg                                                                <  2:830685/140‑1 (MQ=255)                                                                        gTGAAAGAAGTGATAGGCCAGAAATCCAACGGCAACCAGGAAAAAGCGCTCAACTTCCTGACGCTGCACCAGAAGTGGGGTATCCACTCCACCTACAGCGACAACCTGCTGATGCTGACTTTAGGTCGcggtggtccgtt                                                               >  2:98395/1‑138 (MQ=255)                                                                            aagaagTGATAGGCCAGAAATCCAACGGCAACCAGGAAAAAGCGCTCAACTTCCTGACGCTGCACCAGAAGTGGGGTATCCACTCCACCTACAGCGACAACCTGCTGATGCTGACTTTAGGTCGcggtggtccggtggtc                                                           <  1:159616/140‑1 (MQ=255)                                                                                   gATAGGCCAGAAATCCAACGGCAACCAGGAAAAAGCGCTCAACTTCCTGACGCTGCACCAGAAGTGGGGTATCCACTCCACCTACAGCGACAACCTGCTGATGCTGACTTTAGGTCGCGGTGGTCCGGTGGTCTGGTTGa                                                    >  2:195278/1‑140 (MQ=255)                                                                                        gCCAGAAATCCAACGGCAACCAGGAAAAAGCGCTCAACTTCCTGACGCTGCACCAGAAGTGGGGTATCCACTCCACCTACAGCGACAACCTGCTGATGCTGACTTTAGGTCGCGGTGGTCCGGTGGTCTGGTTGAGTGaa                                               >  1:952199/1‑140 (MQ=255)                                                                                             aaaTCCAACGGCAACCAGGAAAAAGCGCTCAACTTCCTGACGCTGCACCAGAAGTGGGGTATCCACTCCACCTACAGCGACAACCTGCTGATGCTGACTTTAGGTCGCGGTGGTCCGGTGGTCTGGTTGAGTGAAGCCGa                                          <  1:1116399/140‑1 (MQ=255)                                                                                                  cAACGGCAACCAGGAAAAAGCGCTCAACTTCCTGACGCTGCACCAGAAGTGGGGTATCCACTCCACCTACAGCGACAACCTGCTGATGCTGACTTTAGGTCGCGGTGGTCCGGTGGTCTGGTTGAGTGAAGCCGATGCCa                                     <  2:952199/140‑1 (MQ=255)                                                                                                    aCGGCAACCAGGAAAAAGCGCTCAACTTCCTGACGCTGCACCAGAAGTGGGGTATCCACTCCACCTACAGCGACAACCTGCTGATGCTGACTTTAGGTCGCGGTGGTCCGGTGGTCTGGTTGAGTGAAGCCGATGCCaaa                                   <  1:441630/140‑1 (MQ=255)                                                                                                           ccAGGAAAAAGCGCTCAACTTCCTGACGCTGCACCAGAAGTGGGGTATCCACTCCACCTACAGCGACAACCTGCTGATGCTGACTTTAGGTCGCGGTGGTCCGGTGGTCTGGTTGAGTGAAGCCGATGCCAAAGATCTgg                            >  2:837144/1‑140 (MQ=255)                                                                                                              ggAAAAAGCGCTCAACTTCCTGACGCTGCACCAGAAGTGGGGTATCCACTCCACCTACAGCGACAACCTGCTGATGCTGACTTTAGGTCGCGGTGGTCCGGTGGTCTGGTTGAGTGAAGCCGATGCCAAAGATCTGGGTa                         <  1:195278/140‑1 (MQ=255)                                                                                                                 aaaaGCGCTCAACTTCCTGACGCTGCACCAGAAGTGGGGTATCCACTCCACCTACAGCGACAACCTGCTGATGCTGACTTTAGGTCGCGGTGGTCCGGTGGTCTGGTTGAGTGAAGCCGATGCCAAAGATCTGGGTATCg                      <  1:680419/140‑1 (MQ=255)                                                                                                                  aaaGCGCTCAACTTCCTGACGCTGCACCAGAAGTGGGGTATCCACTCCACCTACAGCGACAACCTGCTGATGCTGACTTTAGGTCGCGGTGGTCCGGTGGTCTGGTTGAGTGAAGCCGATGCCAAAGATCTGGGTATCGc                     <  2:778361/140‑1 (MQ=255)                                                                                                                      cgcTCAACTTCCTGACGCTGCACCAGAAGTGGGGTATCCACTCCACCTACAGCGACAACCTGCTGATGCTGACTTTAGGTCGCGGTGGTCCGGTGGTCTGGTTGAGTGAAGCCGATGCCAAAGATCTGGGTATCGCCGAt                 <  1:1217739/140‑1 (MQ=255)                                                                                                                         tCAACTTCCTGACGCTGCACCAGAAGTGGGGTATCCACTCCACCTACAGCGACAACCTGCTGATGCTGACTTTAGGTCGCGGTGGTCCGGTGGTCTGGTTGAGTGAAGCCGATGCCAAAGATCTGGGTATCGCCGATAAc              >  2:515380/1‑140 (MQ=255)                                                                                                                                   gACGCTGCACCAGAAGTGGGGTATCCACTCCACCTACAGCGACAACATGCTGATGCTGACTTTAGGTCGCGGTGGTCCGGTGGTCTGGTTGAGTGAAGCCGATGCCAAAGATCTGGGTATCGCCGATAACGACTGGATTg    <  1:1418820/140‑1 (MQ=255)                                                                                                                                     cgctgcACCAGAAGTGGGGTATCCACTCCACCTACAGCGACAACCTGCTGATGCTGACTTTAGGTCGCGGTGGTCCGGTGGTCTGGTTGAGTGAAGCCGATGCCAAAGATCTGGGTATCGCCGATAACGACTGGATTGaa  <  2:1249322/140‑1 (MQ=255)                                                                                                                                        |                                                                                                                                         AGGATCACCAGTGGATGCGTGATTTCGGTGAAAGCCTGCTGGTTTATCGTCCGCCGATCGACACCCGTTCGGTGAAAGAAGTGATAGGCCAGAAATCCAACGGCAACCAGGAAAAAGCGCTCAACTTCCTGACGCCGCACCAGAAGTGGGGTATCCACTCCACCTACAGCGACAACCTGCTGATGCTGACTTTAGGTCGCGGTGGTCCGGTGGTCTGGTTGAGTGAAGCCGATGCCAAAGATCTGGGTATCGCCGATAACGACTGGATTGAA  >  NC_000913/1283003‑1283274

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑