Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,464,089	C→T	A184V (GCC→GTC)	paaX →	DNA‑binding transcriptional repressor PaaX

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,464,089	0	C	T	100.0%	178.4 / NA	57	A184V (GCC→GTC)	paaX	DNA‑binding transcriptional repressor PaaX
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (30/27);  total (30/27)

ACCCAGCCTGATGGCATCGCCGTCGCAAAAACTGGCCGATGTACAGACACTTTTGCATGAAGCGGGTGTGGCGGATAACGTGATTTGTTTTGAAGCGCAAATACCACTGGCGCTTTCTCGCGCAGCACTGCGTGCCAGAGTAGAAGAGTGCTGGCATTTAACTGAACAAAATGCCATGTACGAAACCTTTATTCAGTCATTCCGCCCGCTGGTGCCGCTTTTAAAAGAGGCGGCAGACGAGTTAACCCCGGAGCGGGCATTTCATATTCA  >  NC_000913/1463955‑1464224                                                                                                                                       |                                                                                                                                        aCCCAGCCTGATGGCATCGCCGTCGCAAAAACTGGCCGATGTACAGACACTTTTGCATGAAGCGGGTGTGGCGGATAACGTGATTTGTTTTGAAGCGCAAATACCACTGGCGCTTTCTCGCGCAGCACTGCGTGTCagag                                                                                                                                    <  1:648130/140‑1 (MQ=255)       ccTGATGGCATCGCCGTCGCAAAAACTGGCCGATGTACAGACACTTTTGCATGAAGCGGGTGTGGCGGATAACGTGATTTGTTTTGAAGCGCAAATACCACTGGCGCTTTCTCGCGCAGCACTGCGTGTCAGAGTagaag                                                                                                                              >  2:1140722/1‑140 (MQ=255)       ccTGATGGCATCGCCGTCGCAAAAACTGGCCGATGTACAGACACTTTTGCATGAAGCGGGTGTGGCGGATAACGTGATTTGTTTTGAAGCGCAAATACCACTGGCGCTTTCTCGCGCAGCACTGCGTGTCAGAGTagaag                                                                                                                              >  2:470695/1‑140 (MQ=255)        cTGATGGCATCGCCGTCGCAAAAACTGGCCGATGTACAGACACTTTTGCATGAAGCGGGTGTGGCGGATAACGTGATTTGTTTTGAAGCGCAAATACCACTGGCGCTTTCTCGCGCAGCACTGCGTGTCAGAGTagaaga                                                                                                                             <  1:145904/140‑1 (MQ=255)            tGGCATCGCCGTCGCAAAAACTGGCCGATGTACAGACACTTTTGCATGAAGCGGGTGTGGCGGATAACGTGATTTGTTTTGAAGCGCAAATACCACTGGCGCTTTCTCGCGCAGCACTGCGTGTCAGAGTAGAAGAGTGc                                                                                                                         >  2:926867/1‑140 (MQ=255)                 tCGCCGTCGCAAAAACTGGCCGATGTACAGACACTTTTGCATGAAGCGGGTGTGGCGGATAACGTGATTTGTTTTGAAGCGCAAATACCACTGGCGCTTTCTCGCGCAGCACTGCGTGTCAGAGTAGAAGAGTGCTGGCa                                                                                                                    >  2:1173487/1‑140 (MQ=255)                 tCGCCGTCGCAAAAACTGGCCGATGTACAGACACTTTTGCATGAAGCGGGTGTGGCGGATAACGTGATTTGTTTTGAAGCGCAAATACCACTGGCGCTTTCTCGCGCAGCACTGCGTGTCAGAGTAGAAGAGTGCTGGCa                                                                                                                    >  2:1223271/1‑140 (MQ=255)                  cGCCGTCGCAAAAACTGGCCGATGTACAGACACTTTTGCATGAAGCGGGTGTGGCGGATAACGTGATTTGTTTTGAAGCGCAAATACCACTGGCGCTTTCTCGCGCAGCACTGCGTGTCAGAGTAGAAGAGTGCTGGCAt                                                                                                                   >  1:1373594/1‑140 (MQ=255)                                  ggCCGATGTACAGACACTTTTGCATGAAGCGGGTGTGGCGGATAACGTGATTTGTTTTGAAGCGCAAATACCACTGGCGCTTTCTCGCGCAGCACTGCGTGTCAGAGTAGAAGAGTGCTGGCATTTAACTGAACAAAATg                                                                                                   <  1:1140722/140‑1 (MQ=255)                                   gCCGATGTACAGACACTTTTGCATGAAGCGGGTGTGGCGGATAACGTGATTTGTTTTGAAGCGCAAATACCACTGGCGCTTTCTCGCGCAGCACTGCGTGTCAGAGTAGAAGAGTGCTGGCATTTAACTGAACAAAATGc                                                                                                  >  2:1158934/1‑140 (MQ=255)                                     cGATGTACAGACACTTTTGCATGAAGCGGGTGTGGCGGATAACGTGATTTGTTTTGAAGCGCAAATACCACTGGCGCTTTCTCGCGCAGCACTGCGTGTCAGAGTAGAAGAGTGCTGGCATTTAACTGAACAAAATGCCa                                                                                                >  2:1113102/1‑140 (MQ=255)                                         gTACAGACACTTTTGCATGAAGCGGGTGTGGCGGATAACGTGATTTGTTTTGAAGCGCAAATACCACTGGCGCTTTCTCGCGCAGCACTGCGTGTCAGAGTAGAAGAGTGCTGGCATTTAACTGAACAAAATGCCATGTa                                                                                            <  1:1173487/140‑1 (MQ=255)                                            cAGACACTTTTGCATGAAGCGGGTGTGGCGGATAACGTGATTTGTTTTGAAGCGCAAATACCACTGGCGCTTTCTCGCGCAGCACTGCGTGTCAGAGTAGAAGAGTGCTGGCATTTAACTGAACAAAATGCCATGTACGa                                                                                         <  2:1373594/140‑1 (MQ=255)                                            cAGACACTTTTGCATGAAGCGGGTGTGGCGGATAACGTGATTTGTTTTGAAGCGCAAATACCACTGGCGCTTTCTCGCGCAGCACTGCGTGTCAGAGTAGAAGAGTGCTGGCATTTAACTGAACAAAATGCCATGTACGa                                                                                         <  1:926867/140‑1 (MQ=255)                                                cacTTTTGCATGAAGCGGGTGTGGCGGATAACGTGATTTGTTTTGAAGCGCAAATACCACTGGCGCTTTCTCGCGCAGCACTGCGTGTCAGAGGAGAAGAGTGCTGGCATTTAACTGAACAAAATGCCATGTACGAAAcc                                                                                     >  2:1287416/1‑140 (MQ=255)                                                  cTTTTGCATGAAGCGGGTGTGGCGGATAACGTGATTTGTTTTTAAGCGCAAATACCACTGGCGCTTTCTCGCGCAGCACTGCGTGTCAGAGGAGAAGAGTGCTGGCATTTAACTGAACAAAATGCCa                                                                                                >  2:648637/1‑127 (MQ=255)                                                  cTTTTGCATGAAGCGGGTGTGGCGGATAACGTGATTTGTTTTGAAGCGCAAATACCACTGGCGCTTTCTCGCGCAGCACTGCGTGTCAGAGTAGAAGAGTGCTGGCATTTAACTGAACAAAATGCCa                                                                                                <  2:56761/127‑1 (MQ=255)                                                  cTTTTGCATGAAGCGGGTGTGGCGGATAACGTGATTTGTTTTGAAGCGCAAATACCACTGGCGCTTTCTCGCGCAGCACTGCGTGTCAGAGTAGAAGAGTGCTGGCATTTAACTGAACAAAATGCCa                                                                                                <  1:648637/127‑1 (MQ=255)                                                  cTTTTGCATGAAGCGGGTGTGGCGGATAACGTGATTTGTTTTGAAGCGCAAATACCACTGGCGCTTTCTCGCGCAGCACTGCGTGTCAGAGTAGAAGAGTGCTGGCATTTAACTGAACAAAATGCCa                                                                                                >  1:56761/1‑127 (MQ=255)                                                  cTTTTGCATGAAGCGGGTGTGGCGGATAACGTGATTTGTTTTGAAGCGCAAATACCACTGGCGCTTTCTCGCGCAGCACTGCGTGTCAGAGTAGAAGAGTGCTGGCATTTAACTGAACAAAATGCCATGTACGAAACCtt                                                                                   >  1:1192930/1‑140 (MQ=255)                                                   ttttGCATGAAGCGGGTGTGGCGGATAACGTGATTTGTTTTGAAGCGCAAATACCACTGGCGCTTTCTCGCGCAGCACTGCGTGTCAGAGTAGAAGAGTGCTGGCATTTAACTGAACAAAATGCCATGTACGAAACCttt                                                                                  >  1:937028/1‑140 (MQ=255)                                                   ttttGCATGAAGCGGGTGTGGCGGATAACGTGATTTGTTTTGAAGCGCAAATACCACTGGCGCTTTCTCGCGCAGCACTGCGTGTCAGAGTAGAAGAGTGCTGGCATTTAACTGAACAAAATGCCATGTACGAAACCttt                                                                                  >  1:1394721/1‑140 (MQ=255)                                                    tttGCATGAAGCGGGTGTGGCGGATAACGTGATTTGTTTTGAAGCGCAAATACCACTGGCGCTTTCTCGCGCAGCACTGCGTGTCAGAGTAGAAGAGTGCTGGCATTTAACTGAACAAAATGCCATGTACGAAACCTTTa                                                                                 >  1:259287/1‑140 (MQ=255)                                                    tttGCATGAAGCGGGTGTGGCGGATAACGTGATTTGTTTTGAAGCGCAAATACCACTGGCGCTTTCTCGCGCAGCACTGCGTGTCAGAGTAGAAGAGTGCTGGCATTTAACTGAACAAAATGCCATGTACGAAACCTTTa                                                                                 >  1:845851/1‑140 (MQ=255)                                                    tttGCATGAAGCGGGTGTGGCGGATAACGTGATTTGTTTTGAAGCGCAAATACCACTGGCGCTTTCTCGCGCAGCACTGCGTGTAAGAGTAGAAGAGTGCTGGCATTTAACTGAACAAAATGCCATGTACGAAACCTTTa                                                                                 <  1:553923/140‑1 (MQ=255)                                                        cATGAAGCGGGTGTGGCGGATAACGTGATTTGTTTTGAAGCGCAAATACCACTGGCGCTTTCTCGCGCAGCACTGCGTGTCAGAGTAGAAGAGTGCTGGCATTTAACTGAACAAAATGCCATGTACGAAACCTTTATTCa                                                                             <  1:1223271/140‑1 (MQ=255)                                                             aGCGGGTGTGGCGGATAACGTGATTTGTTTTGAAGCGCAAATACCACTGGCGCTTTCTCGCGCAGCACTGCGTGTCAGAGTAGAAGAGTGCTGGCATTTAACTGAACAAAATGCCATGTACGAAACCTTTATTCAGTCAt                                                                        >  2:1105161/1‑140 (MQ=255)                                                                       gCGGATAACGTGATTTGTTTTGAAGCGCAAATACCACTGGCGCTTTCTCGCGCAGCACTGCGTGTCAGAGTAGAAGAGTGCTGGCATTTAACTGAACAAAATGCCATGTACGAAACCTTTATTCAGTCATTCCGCCCGCt                                                              >  1:452864/1‑140 (MQ=255)                                                                       gCGGATAACGTGATTTGTTTTGAAGCGCAAATACCACTGGCGCTTTCTCGCGCAGCACTGCGTGTCAGAGTAGAAGAGTGCTGGCATTTAACTGAACAAAATGCCATGTACGAAACCTTTATTCAGTCATTCCGCCCGCt                                                              >  1:245839/1‑140 (MQ=255)                                                                       gCGGATAACGTGATTTGTTTTGAAGCGCAAATACCACTGGCGCTTTCTCGCGCAGCACTGCGTGTCAGAGTAGAAGAGTGCTGGCATTTAACTGAACAAAATGCCATGTACGAAACCCTTATTCAGTCATTCCGCCCGCt                                                              >  1:760096/1‑140 (MQ=255)                                                                            tAACGTGATTTGTTTTGAAGCGCAAATACCACTGGCGCTTTCTCGCGCAGCACTGCGTGTCAGAGTAGAAGAGTGCTGGCATTTAACTGAACAAAATGCCATGTACGAAACCTTTATTCAGTCATTCCGCCCGCTGGTGc                                                         <  1:729455/140‑1 (MQ=255)                                                                            tAACGTGATTTGTTTTGAAGCGCAAATACCACTGGCGCTTTCTCGCGCAGCACTGCGTGTCAGAGTAGAAGAGTGCTGGCATTTAACTGAACAAAATGCCATGTACGAAACCTTTATTCAGTCATTCCGCCCGCTGGTGc                                                         >  2:901658/1‑140 (MQ=255)                                                                             aaCGTGATTTGTTTTGAAGCGCAAATACCACTGGCGCTTTCTCGCGCAGCACTGCGTGTCAGAGTAGAAGAGTGCTGGCATTTAACTGAACAAAATGCCATGTACGAAACCTTTATTCAGTCATTCCGCCCGCTGGTGcc                                                        >  2:403666/1‑140 (MQ=255)                                                                                  gATTTGTTTTGAAGCGCAAATACCACTGGCGCTTTCTCGCGCAGCACTGCGTGTCAGAGTAGAAGAGTGCTGGCATTTAACTGAACAAAATGCCATGTACGAAACCTTTATTCAGTCATTCCGCCCGCTGGTGCCGCttt                                                   >  2:562023/1‑140 (MQ=255)                                                                                       gTTTTGAAGCGCAAATACCACTGGCGCTTTCTCGCGCAGCACTGCGTGTCAGAGTAGAAGAGTGCTGGCATTTAACTGAACAAAATGCCATGTACGAAACCTTTATTCAGTCATTCCGCCCGCTGGTGCCGCTTTTaaaa                                              <  2:1405439/140‑1 (MQ=255)                                                                                                  cAAATACCACTGGCGCTTTCTCGCGCAGCACTGCGTGTCAGAGTAGAAGAGTGCTGGCATTTAACTGAACAAAATGCCATGTACGAAACCTTTATTCAGTCATTCCGCCCGCTGGTGCCGCTTTTAAAAGAGGCGGCAGa                                   >  1:316424/1‑140 (MQ=255)                                                                                                  cAAATACCACTGGCGCTTTCTCGCGCAGCACTGCGTGTCAGAGTAGAAGAGTGCTGGCATTTAACTGAACAAAATGCCATGTACGAAACCTTTATTCAGTCATTCCGCCCGCTGGTGCCGCTTTTAAAAGAGGCGGCAGa                                   <  1:562023/140‑1 (MQ=255)                                                                                                  cAAATACCACTGGCGCTTTCTCGCGCAGCACTGCGTGTCAGAGTAGAAGAGTGCTGGCATTTAACTGAACAAAATGCCATGTACGAAACCTTTATTCAGTCATTCCGCCCGCTGGTGCCGCTTTTAAAAGAGGCGGCAGa                                   >  1:972557/1‑140 (MQ=255)                                                                                                  cAAATACCACTGGCGCTTTCTCGCGCAGCACTGCGTGTCAGAGTAGAAGAGTGCTGGCATTTAACTGAACAAAATGCCATGTACGAAACCCTTATTCAGTCATTCCGCCCGCTGGTGCCGCTTTTAAAAGAGGCGGCAGa                                   <  2:760096/140‑1 (MQ=255)                                                                                                        ccACTGGCGCTTTCTCGCGCAGCACTGCGTGTCAGAGTAGAAGAGTGCTGGCATTTAACTGAACAAAATGCCATGTACGAAACCTTTATTCAGTCATTCCGCCCGCTGGTGCCGCTTTTAAAAGAGGCGGCAGACGAGtt                             <  1:658904/140‑1 (MQ=255)                                                                                                           cTGGCGCTTTCTCGCGCAGCACTGCGTGTCAGAGTAGAAGAGTGCTGGCATTTAACTGAACAAAATGCCATGTACGAAACCTTTATTCAGTCATTCCGCCCGCTGGTGCCGCTTTTAAAAGAGGCGGCAGa                                   <  2:1293602/131‑1 (MQ=255)                                                                                                           cTGGCGCTTTCTCGCGCAGCACTGCGTGTCAGAGTAGAAGAGTGCTGGCATTTAACTGAACAAAATGCCATGTACGAAACCTTTATTCAGTCATTCCGCCCGCTGGTGCCGCTTTTAAAAGAGGCGGCAGa                                   >  1:1293602/1‑131 (MQ=255)                                                                                                                 cTTTCTCGCGCAGCACTGCGTGTCAGAGTAGAAGAGTGCTGGCATTTAACTGAACAAAATGCCATGTACGAAACCTTTATTCAGTCATTCCGCCCGCTGGTGCCGCTTTTAAAAGAGGCGGCAGACGAGTTAACCCCGGa                    >  2:1275028/1‑140 (MQ=255)                                                                                                                  tttCTCGCGCAGCACTGCGTGTCAGAGTAGAAGAGTGCTGGCATTTAACTGAACAAAATGCCATGTACGAAACCTTTATTCAGTCATTCCGCCCGCTGGTGCCGCTTTTAAAAGAGGCGGCAGACGAGTTAACCCCGGAg                   <  1:1158934/140‑1 (MQ=255)                                                                                                                    tctcGCGCAGCACTGCGTGTCAGAGTAGAAGAGTGCTGGCATTTAACTGAACAAAATGCCATGTACGAAACCTTTATTCAGTCATTCCGCCCGCTGGTGCCGCTTTTAAAAGAGGCGGCAGACGAGTTAACCCCGGAGCg                 >  1:26722/1‑140 (MQ=255)                                                                                                                    tctcGCGCAGCACTGCGTGTCAGAGTAGAAGAGTGCTGGCATTTAACTGAACAAAATGCCATGTACGAAACCTTTATTCAGTCATTCCGCCCGCTGGTGCCGCTTTTAAAAGAGGCGGCAGACGAGTTAACCCCGGAGCg                 <  2:845851/140‑1 (MQ=255)                                                                                                                            agcaCTGCGTGTCAGAGTAGAAGAGTGCTGGCATTTAACTGAACAAAATGCCATGTACGAAACCTTTATTCAGTCATTCCGCCCGCTGGTGCCGCTTTTAAAAGAGGCGGCAGACGAGTTAACCCCGGAGCGGGCATTTc         <  2:1394721/140‑1 (MQ=255)                                                                                                                            agcaCTGCGTGTCAGAGTAGAAGAGTGCTGGCATTTAACTGAACAAAATGCCATGTACGAAACCTTTATTCAGTCATTCCGCCCGCTGGTGCCGCTTTTAAAAGAGGCGGCAGACGAGTTAACCCCGGAGCGGGCATTTc         <  2:951183/140‑1 (MQ=255)                                                                                                                              caCTGCGTGTCAGAGTAGAAGAGTGCTGGCATTTAACTGAACAAAATGCCATGTACGAAACCTTTATTCAGTCATTCCGCCCGCTGGTGCCGCTTTTAAAAGAGGCGGCAGACGAGTTAACCCCGGAGCGGGc              <  1:855093/133‑1 (MQ=255)                                                                                                                              caCTGCGTGTCAGAGTAGAAGAGTGCTGGCATTTAACTGAACAAAATGCCATGTACGAAACCTTTATTCAGTCATTCCGCCCGCTGGTGCCGCTTTTAAAAGAGGCGGCAGACGAGTTAACCCCGGAGCGGGc              >  2:855093/1‑133 (MQ=255)                                                                                                                              caCTGCGTGTCAGAGTAGAAGAGTGCTGGCATTTAACTGAACAAAATGCCATGTACGAAACCTTTATTCAGTCATTCCGCCCGCTGGTGCCGCTTTTAAAAGAGGCGGCAGACGAGTTAACCCCGGAGCGGGCATTTCat       <  2:972557/140‑1 (MQ=255)                                                                                                                              caCTGCGTGTCAGAGTAGAAGAGTGCTGGCATTTAACTGAACAAAATGCCATGTACGAAACCTTTATTCAGTCATTCCGCCCGCTGGTGCCGCTTTTAAAAGAGGCGGCAGACGAGTTAACCCCGGAGCGGGCATTTCat       >  1:533298/1‑140 (MQ=255)                                                                                                                                cTGCGTGTCAGAGTAGAAGAGTGCTGGCATTTAACTGAACAAAATGCCATGTACGAAACCTTTATTCAGTCATTCCGCCCGCTGGTGCCGCTTTTAAAAGAGGCGGCAGACGAGTTAACCCCGGAGCGGGCATTTCatat     <  1:1275028/140‑1 (MQ=255)                                                                                                                                cTGCGTGTCAGAGTAGAAGAGTGCTGGCATTTAACTGAACAAAATGCCATGTACGAAACCTTTATTCAGTCATTCCGCCCGCTGGTGCCGCTTTTAAAAGAGGCGGCAGACGAGTTAACCCCGGAGCGGGCATTTCatat     <  2:452864/140‑1 (MQ=255)                                                                                                                                cTGCGTGTCAGAGTAGAAGAGTGCTGGCATTTAACTGAACAAAATGCCATGTACGAAACCTTTATTCAGTCATTCCGCCCGCTGGTGCCGCTTTTAAAAGAGGCGGCAGACGAGTTAACCCCGGAGCGGGCATTTCatat     <  2:937028/140‑1 (MQ=255)                                                                                                                                cTGCGTGTCAGAGTAGAAGAGTGCTGGCATTTAACTGAACAAAATGCCATGTACGAAACCTTTATTCAGTCATTCCGCCCGCTGGTGCCGCTTTTAAAAGAGGCGGCAGACGAGTTAACCCCGGAGCGGGCATTTCatat     <  2:26722/140‑1 (MQ=255)                                                                                                                                cTGCGTGTCAGAGTAGAAGAGTGCTGGCATTTAACTGAACAAAATGCCATGTACGAAACCTTTATTCAGTCATTCCGCCCGCTGGTGCCGCTTTTAAAAGAGGCGGCAGACGAGTTAACCCCGGAGCGGGCATTTCatat     <  1:403666/140‑1 (MQ=255)                                                                                                                                 tGCGTGTCAGAGTAGAAGAGTGCTGGCATTTAACTGAACAAAATGCCATGTACGAAACCTTTATTCAGTCATTCCGCCCGCTGGTGCCGCTTTTAAAAGAGGCGGCAGACGAGTTAACCCCGGAGCGGGCATTTCATAtt    <  1:1105161/140‑1 (MQ=255)                                                                                                                                   cGTGTCAGAGTAGAAGAGTGCTGGCATTTAACTGAACAAAATGCCATGTACGAAACCTTTATTCAGTCATTCCGCCCGCTGGTGCCGCTTTTAAAAGAGGCGGCAGACGAGTTAACCCCGGAGCGGGCATTTCATATTCa  >  2:630015/1‑140 (MQ=255)                                                                                                                                       |                                                                                                                                        ACCCAGCCTGATGGCATCGCCGTCGCAAAAACTGGCCGATGTACAGACACTTTTGCATGAAGCGGGTGTGGCGGATAACGTGATTTGTTTTGAAGCGCAAATACCACTGGCGCTTTCTCGCGCAGCACTGCGTGCCAGAGTAGAAGAGTGCTGGCATTTAACTGAACAAAATGCCATGTACGAAACCTTTATTCAGTCATTCCGCCCGCTGGTGCCGCTTTTAAAAGAGGCGGCAGACGAGTTAACCCCGGAGCGGGCATTTCATATTCA  >  NC_000913/1463955‑1464224

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑