Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	179,819	A→G	T195A (ACA→GCA)	dgt →	dGTP triphosphohydrolase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	179,819	0	A	G	100.0%	208.0 / NA	56	T195A (ACA→GCA)	dgt	dGTP triphosphohydrolase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (27/29);  total (27/29)

TCTGACTGACGATCGCTGCAGCGTGGCGGCACTACGTTTACGGGACGGGGAAGAACCGCTTAACGAGCTGCGGCGCAAGATTCGTCAGGACTTATGTCATTTTGAGGGGAATGCACAAGGCATTCGCCTGGTGCATACATTGATGCGGATGAATCTCACCTGGGCACAGGTTGGCGGTATTTTAAAATATACCCGTCCGGCGTGGTGGCGTGGCGAAACGCCTGAGACACATCACTATTTAATGAAAAAGCCGGGTTATTATCTTTCTGAA  >  NC_000913/179683‑179953                                                                                                                                         |                                                                                                                                       tctGACTGACGATCGCTGCAGCGTGGCGGCACTACGTTTACGGGACGGGGAAGAACCGCTTAACGAGCTGCGGCGCAAGATTCGTCAGGACTTATGTCATTTTGAGGGGAATGCACAAGGCATTCGCCTGGTgcatgcat                                                                                                                                     <  2:450614/140‑1 (MQ=255)         acGATCGCTGCAGCGTGGCGGCACTACGTTTACGGGACGGGGAAGAACCGCTTAACGAGCTGCGGCGCAAGATTCGTCAGGACTTATGTCATTTTGAGGGGAATGCACAAGGCATTCGCCTGGTGCATGCATTGATGCgg                                                                                                                             <  1:649160/140‑1 (MQ=255)          cGATCGCTGCAGCGTGGCGGCACTACGTTTACGGGACGGGGAAGAACCGCTTAACGAGCTGCGGCGCAAGATTCGTCAGGACTTATGTCATTTTGAGGGGAATGCACAAGGCATTCGCCTGGTGCATGCATTGATGCGGa                                                                                                                            >  1:758408/1‑140 (MQ=255)          cGATCGCTGCAGCGTGGCGGCACTACGTTTACGGGACGGGGAAGAACCGCTTAACGAGCTGCGGCGCAAGATTCGTCAGGACTTATGTCATTTTGAGGGGAATGCACAAGGCATTCGCCTGGTGCATGCATTGATGCGGa                                                                                                                            <  1:482492/140‑1 (MQ=255)           gATCGCTGCAGCGTGGCGGCACTACGTTTACGGGACGGGGAAGAACCGCTTAACGAGCTGCGGCGCAAGATTCGTCAGGACTTATGTCATTTTGAGGGGAATGCACAAGGCATTCGCCTGGTGCATGCATTGATGCGGAt                                                                                                                           >  2:305453/1‑140 (MQ=255)            aTCGCTGCAGCGTGGCGGCACTACGTTTACGGGACGGGGAAGAACCGCTTAACGAGCTGCGGCGCAAGATTCGTCAGGACTTATGTCATTTTGAGGGGAATGCACAAGGCATTCGCCTGGTGCATGCATTGATGCGGATg                                                                                                                          >  2:1344287/1‑140 (MQ=255)               gCTGCAGCGTGGCGGCACTACGTTTACGGGACGGGGAAGAACCGCTTAACGAGCTGCGGCGCAAGATTCGTCAGGACTTATGTCATTTTGAGGGGAATGCACAAGGCATTCGCCTGGTGCATGCATTGATGCGGATGAAt                                                                                                                       <  1:1073563/140‑1 (MQ=255)                   cAGCGTGGCGGCACTACGTTTACGGGACGGGGAAGAACCGCTTAACGAGCTGCGGCGCAAGATTCGTCAGGACTTATGTCATTTTGAGGGGAATGCACAAGGCATTCGCCTGGTGCATGCATTGATGCGGATGAATTTCa                                                                                                                   >  1:412963/1‑140 (MQ=255)                    aGCGTGGCGGCACTACGTTTACGGGACGGGGAAGAACCGCTTAACGAGCTGCGGCGCAAGATTCGTCAGGACTTATGTCATTTTGAGGGGAATGCACAAGGCATTCGCCTGGTGCATGCATTGATGCGGATGAATCTCAc                                                                                                                  >  1:1404709/1‑140 (MQ=255)                    aGCGTGGCGGCACTACGTTTACGGGACGGGGAAGAACCGCTTAACGAGCTGCGGCGCAAGATTCGTCAGGACTTATGTCATTTTGAGGGGAATGCACAAGGCATTCGCCTGGTGCATGCATTGATGCGGATGAATCTCAc                                                                                                                  <  2:146082/140‑1 (MQ=255)                      cGTGGCGGCACTACGTTTACGGGACGGGGAAGAACCGCTTAACGAGCTGCGGCGCAAGATTCGTCAGGACTTATGTCATTTTGAGGGGAATGCACAAGGCATTCGCCTGGTGCATGCATTGATGCGGATGAATCTCACCt                                                                                                                >  1:3343/1‑140 (MQ=255)                        tGGCGGCACTACGTTTACGGGACGGGGAAGAACCGCTTAACGAGCTGCGGCGCAAGATTCGTCAGGACTTATGTCATTTTGAGGGGAATGCACAAGGCATTCGCCTGGTGCATGCATTGATGCGGATGAATCTCACCTgg                                                                                                              >  1:259321/1‑140 (MQ=255)                        tGGCGGCACTACGTTTACGGGACGGGGAAGAACCGCTTAACGAGCTGCGGCGCAAGATTCGTCAGGACTTATGTCATTTTGAGGGGAATGCACAAGGCATTCGCCTGGTGCATGCATTGATGCGGATGAATCTCACCTgg                                                                                                              >  1:307456/1‑140 (MQ=255)                         ggcggcACTACGTTTACGGGACGGGGAAGAACCGCTTAACGAGCTGCGGCGCAAGATTCGTCAGGACTTATGTCATTTTGAGGGGAATGCACAAGGCATTCGCCTGGTGCATGCATTGATGCGGATGAATCTCACCTggg                                                                                                             <  1:601653/140‑1 (MQ=255)                           cggcACTACGTTTACGGGACGGGGAAGAACCGCTTAACGAGCTGCGGCGCAAGATTCGTCAGGACTTATGTCATTTTGAGGGGAATGCACAAGGCATTCGCCTGGTGCATGCATTGATGCGGATGAATCTCACCTGGGca                                                                                                           >  1:723416/1‑140 (MQ=255)                           cggcACTACGTTTACGGGACGGGGAAGAACCGCTTAACGAGCTGCGGCGCAAGATTCGTCAGGACTTATGTCATTTTGAGGGGAATGCACAAGGCATTCGCCTGGTGCATGCATTGATGCGGATGAATCTCACCTGGGca                                                                                                           <  2:1393284/140‑1 (MQ=255)                           cggcACTACGTTTACGGGACGGGGAAGAACCGCTTAACGAGCTGCGGCGCAAGATTCGTCAGGACTTATGTCATTTTGAGGGGAATGCACAAGGCATTCGCCTGGTGCATGCATTGATGCGGATGAATCTCACCTGGGca                                                                                                           >  2:302349/1‑140 (MQ=255)                                   cGTTTACGGGACGGGGAAGAACCGCTTAACGAGCTGCGGCGCAAGATTCGTCAGGACTTATGTCATTTTGAGGGGAATGCACAAGGCATTCGCCTGGTGCATGCATTGATGCGGATGAATCTCACCTGGGCACAGGt                                                                                                      <  2:1286357/137‑1 (MQ=255)                                   cGTTTACGGGACGGGGAAGAACCGCTTAACGAGCTGCGGCGCAAGATTCGTCAGGACTTATGTCATTTTGAGGGGAATGCACAAGGCATTCGCCTGGTGCATGCATTGATGCGGATGAATCTCACCTGGGCACAGGt                                                                                                      >  1:1286357/1‑137 (MQ=255)                                     tttACGGGACGGGGAAGAACCGCTTAACGAGCTGCGGCGCAAGATTCGTCAGGACTTATGTCATTTTGAGGGGAATGCACAAGGCATTCGCCTGGTGCATGCATTGATGCGGATGAATCTCACCTGGGCACAGGTTGGCg                                                                                                 <  1:1416019/140‑1 (MQ=255)                                      ttACGGGACGGGGAAGAACCGCTTAACGAGCTGCGGCGCAAGATTCGTCAGGACTTATGTCATTTTGAGGGGAATGCACAAGGCATTCGCCTGGTGCATGCATTGATGCGGATGAATCTCACCTGGGCACAGGTTGGCgg                                                                                                <  2:758408/140‑1 (MQ=255)                                      ttACGGGACGGGGAAGAACCGCTTAACGAGCTGCGGCGCAAGATTCGTCAGGACTTATGTCATTTTGAGGGGAATGCACAAGGCATTCGCCTGGTGCATGCATTGATGCGGATGAATCTCACCTGGGCACAGGTTGGCgg                                                                                                <  1:305453/140‑1 (MQ=255)                                      ttACGGGACGGGGAAGAACCGCTTAACGAGCTGCGGCGCAAGATTCGTCAGGACTTATGTCATTTTGAGGGGAATGCACAAGGCATTCGCCTGGTGCATGCATTGATGCGGATGAATCTCACCTGGGCACAGGTTGGCgg                                                                                                <  2:307456/140‑1 (MQ=255)                                           ggacgggGAAGAACCGCTTAACGAGCTGCGGCGCAAGATTCGTCAGGACTTATGTCATTTTGAGGGGAATGCACAAGGCATTCGCCTGGTGCATGCATTGATGCGGATGAATCTCACCTGGTCACAGGTTGGCGGTAttt                                                                                           <  2:1404709/140‑1 (MQ=255)                                             acgggGAAGAACCGCTTAACGAGCTGCGGCGCAAGATTCGTCAGGACTTATGTCATTTTGAGGGGAATGCACAAGGCATTCGCCTGGTGCATGCATTGATGCGGATGAATCTCACCTGGGCACAGGTTGGCGGTATTTTa                                                                                         >  2:1297292/1‑140 (MQ=255)                                              cgggGAAGAACCGCTTAACGAGCTGCGGCGCAAGATTCGTCAGGACTTATGTCATTTTGAGGGGAATGCACAAGGCATTCGCCTGGTGCATGCATTGATGCGGATGAATCTCACCTGGGCACAGGTTGGCGGTATTTTaa                                                                                        >  1:98628/1‑140 (MQ=255)                                                gggAAGAACCGCTTAACGAGCTGCGGCGCAAGATTCGTCAGGACTTATGTCATTTTGAGGGGAATGCACAAGGCATTCGCCTGGTGCATGCATTGATGCGGATGAATCTCACCTGGGCACAGGTTGGCGGTATTTTaaaa                                                                                      <  1:1094148/140‑1 (MQ=255)                                                 ggAAGAACCGCTTAACAAGCTGCGGCGCAAGATTCGTCAGGACTTATGTCATTTTGAGGGGAATGCACAAGGCATTCGCCTGGTGCATGCATTGATGCGGATGAATCTCACCTGGGCACAGGTTGGCGGTATTTTAAAat                                                                                     >  2:330593/1‑140 (MQ=255)                                                           cTTAACGAGCTGCGGCGCAAGATTCGTCAGGACTTATGTCATTTTGAGGGGAATGCACAAGGCATTCGCCTGGTGCATGCATTGATGCGGATGAATCTCACCTGGGCACAGGTTGGCGGTATTTTAAAATATACCCGTcc                                                                           >  1:698157/1‑140 (MQ=255)                                                           cTTAACGAGCTGCGGCGCAAGATTCGTCAGGACTTATGTCATTTTGAGGGGAATGCACAAGGCATTCGCCTGGTGCATGCATTGATGCGGATGAATCTCACCTGGGCACAGGTTGGCGGTATTTTAAAATATACCCGTcc                                                                           >  1:702624/1‑140 (MQ=255)                                                                    cTGCGGCGCAAGATTCGTCAGGACTTATGTCATTTTGAGGGGAATGCACAAGGCATTCGCCTGGTGCATGCATTGATGCGGATGAATCTCACCTGGGCACAGGTTGGCGGTATTTTAAAATATACCCGTCCGGCgtggtg                                                                  <  2:98628/140‑1 (MQ=255)                                                                         gcgcAAGATTCGTCAGGACTTATGTCATTTTGAGGGGAATGCACAAGGCATTCGCCTGGTGCATGCATTGATGCGGATGAATCTCACCTGGGCACAGGTTGGCGGTATTTTAAAATATACCCGTCCGGCGTGgtggcgtg                                                             >  2:1328619/1‑140 (MQ=255)                                                                              aGATTCGTCAGGACTTATGTCATTTTGAGGGGAATGCACAAGGCATTCGCCTGGTGCATGCATTGATGCGGATGAATTTCACCTGGGCACAGGTTGGCGGTATTTTAAAATATACCCGTCCGGCGTGGTGGCGTGGCGaa                                                        <  2:412963/140‑1 (MQ=255)                                                                              aGATTCGTCAGGACTTATGTCATTTTGAGGGGAATGCACAAGGCATTCGCCTGGTGCATGCATTGATGCGGATGAATCTCACCTGGGCACAGGTTGGCGGTATTTTAAAATATACCCGTCCGGCGTGGTGGCGTGGCGaa                                                        <  1:1297292/140‑1 (MQ=255)                                                                              aGATTCGTCAGGACTTATGTCATTTTGAGGGGAATGCACAAGGCATTCGCCTGGTGCATGCATTGATGCGGATGAATCTCACCTGGGCACAGGTTGGCGGTATTTTAAAATATACCCGTCCGGCGTGGTGGCGTGGCGaa                                                        <  2:259321/140‑1 (MQ=255)                                                                                     tCAGGACTTATGTCATTTTGAGGGGAATGCACAAGGCATTCGCCTGGTGCATGCATTGATGCGGATGAATCTCACCTGGGCACAGGTTGGCGGTATTTTAAAATATACCCGTCCGGCGTGGTGGCGTGGCGAAACGCCTg                                                 <  2:3343/140‑1 (MQ=255)                                                                                        ggACTTATGTCATTTTGAGGGGAATGCACAAGGCATTCGCCTGGTGCATGCATTGATGCGGATGAATCTCACCTGGGCACAGGTTGGCGGTATTTTAAAATATACCCGTCCGGCGTGGTGGCGTGGCGAAACGCCTgaga                                              <  1:576480/140‑1 (MQ=255)                                                                                         gACTTATGTCATTTTGAGGGGAATGCACAAGGCATTCGCCTGGTGCATGCATTGATGCGGATGAATCTCACCTGGGCACAGGTTGGCGGTATTTTAAAATATACCCGTCCGGCGTGGTGGCGTGGCGAAACGCCTGAGac                                             >  2:767115/1‑140 (MQ=255)                                                                                          gcTTATGTCATTTTGAGGGGAATGCACAAGGCATTCGCCTGGTGCATGCATTGATGCGGATGAATCTCACCTGGGCACAGGTTGGCGGTATTTTAAAATATACCCGTCCGGCGTGGTGGCGTGGCGAAACGCCTGAGaca                                            <  2:814609/139‑1 (MQ=255)                                                                                          aCTTATGTCATTTTGAGGGGAATGCACAAGGCATTCGCCTGGTGCATGCATTGATGCGGATGAATCTCACCTGGGCACAGGTTGGCGGTATTTTAAAATATACCCGTCCGGCGTGGTGGCGTGGCGAAACGCCTGAGaca                                            <  2:698157/140‑1 (MQ=255)                                                                                              aTGTCATTTTGAGGGGAATGCACAAGGCATTCGCCTGGTGCATGCATTGATGCGGATGAATCTCACCTGGGCACAGGTTGGCGGTATTTTAAAATATACCCGTCCGGCGTGGTGGCGTGGCGAAACGCCTGAGACACATc                                        >  1:573604/1‑140 (MQ=255)                                                                                               tGTCATTTTGAGGGGAATGCACAAGGCATTCGCCTGGTGCATGCATTGATGCGGATGAATCTCACCTGGGCACAGGTTGGCGGTATTTTAAAATATACCCGTCCGGCGTGGTGGCGTGGCGAAACGCCTGAGACACATCa                                       <  2:702624/140‑1 (MQ=255)                                                                                               tGTCATTTTGAGGGGAATGCACAAGGCATTCGCCTGGTGCATGCATTGATGCGGATGAATCTCACCTGGGCAAAGGTTGGGGGTATTTTAAAATATACCCGTCCGGCGTGGTGGCGTGGCGAAACGCCTGAGACACATCa                                       <  2:397934/140‑1 (MQ=255)                                                                                                    ttttGAGGGGAATGCACAAGGCATTCGCCTGGTGCATGCATTGATGCGGATGAATCTCACCTGGGCACAGGTTGGCGGTATTTTAAAATATACCCGTCCGGCGTGGTGGCGTGGCGAAACGCCTGAGACACATCa                                       <  1:1008783/135‑1 (MQ=255)                                                                                                    ttttGAGGGGAATGCACAAGGAATTCGCCTGGTGCATGCATTGATGCGGATGAATCTCACCTGGGCACAGGTTGGCGGTATTTTAAAATATACCCGTCCGGCGTGGTGGCGTGGCGAAACGCCTGAGACACATCa                                       >  2:1008783/1‑135 (MQ=255)                                                                                                                tGCACAAGGCATTCGCCTGGTGCATGCATTGATGCGGATGAATCTCACCTGGGCACAGGTTGGCGGTATTTTAAAATATACCCGTCCGGCGTGGTGGCGTGGCGAAACGCCTGAGACACATCACTATTTAATGAAAAAGc                      >  2:432709/1‑140 (MQ=255)                                                                                                                 gCACAAGGCATTCGCCTGGTGCATGCATTGATGCGGATGAATCTCACCTGGGCACAGGTTGGCGGTATTTTAAAATATACCCGTCCGGCGTGGTGGCGTGGCGAAACGCCTGAGACACATCACTATTTAATGAAAAAGcc                     <  1:227761/140‑1 (MQ=255)                                                                                                                 gCACAAGGCATTCGCCTGGTGCATGCATTGATGCGGATGAATCTCACCTGGGCACAGGTTGGCGGTATTTTAAAATATACCCGTCCGGCGTGGTGGCGTGGCGAAACGCCTGAGACACATCACTATTTAATGAAAAAGcc                     <  2:641303/140‑1 (MQ=255)                                                                                                                   acaAGGCATTCGCCTGGTGCATGCATTGATGCGGATGAATCTCACCTGGGCACAGGTTGGCGGTATTTTAAAATATACCCGTCCGGCGTGGTGGCGTGGCGAAACGCCTGAGACACATCACTATTTAATGAAAAAGc                      >  2:655313/1‑137 (MQ=255)                                                                                                                   acaAGGCATTCGCCTGGTGCATGCATTGATGCGGATGAATCTCACCTGGGCACAGGTTGGCGGTATTTTAAAATATACCCGTCCGGCGTGGTGGCGTGGCGAAACGCCTGAGACACATCACTATTTAATGAAAAAGc                      <  1:655313/137‑1 (MQ=255)                                                                                                                       ggCATTCGCCTGGTGCATGCATTGATGCGGATGAATCTCACCTGGGCACAGGTTGGCGGTATTTTAAAATATACCCGTCCGGCGTGGTGGCGTGGCGAAACGCCTGAGACACATCACTATTTAATGAAAAAGCCGGGtta               >  1:1384065/1‑140 (MQ=255)                                                                                                                       ggCATTCGCCTGGTGCATGCATTGATGCGGATGAATCTCACCTGGGCACAGGTTGGCGGTATTTTAAAATATACCCGTCCGGCGTGGTGGCGTGGCGAAACGCCTGAGACACATCACTATTTAATGAAAAAGCCGGGtta               >  1:937285/1‑140 (MQ=255)                                                                                                                          aTTCGCCTGGTGCATGCATTGATGCGGATGAATCTCACCTGGGCACAGGTTGGCGGTATTTTAAAATATACCCGTCCGGCGTGGTAGCGTGGCGAAACGCCTGAGACACATCACTATTTAATGAAAAAGCCGGGttatta            <  1:302349/140‑1 (MQ=255)                                                                                                                            tCGCCTGGTGCATGCATTGATGCGGATGAATCTCACCTGGGCACAGGTTGGCGGTATTTTAAAATATACCCGTCCGGCGTGGTGGCGTGGCGAAACGCCTGAGACACATCACTATTTAATGaaa                          <  2:632762/124‑1 (MQ=255)                                                                                                                            tCGCCTGGTGCATGCATTGATGCGGATGAATCTCACCTGGGCACAGGTTGGCGGTATTTTAAAATATACCCGTCCGGCGTGGTGGCGTGGCGAAACGCCTGAGACACATCACTATTTAATGaaa                          >  1:632762/1‑124 (MQ=255)                                                                                                                            tCGCCTGGTGCATGCATTGATGCGGATGAATCTCACCTGGGCACAGGTTGGCGGTATTTTAAAATATACCCGTCCGGCGTGGTGGCGTGGCGAAACGCCTGAGACACATCACTATTTAATGAAAAAGCCGGGTTATTATc          >  1:532892/1‑140 (MQ=255)                                                                                                                            tCGCCTGGTGCATGCATTGATGCGGATGAATCTCACCTGGGCACAGGTTGGCGGTATTTTAAAATATACCCGTCCGGCGTGGTGGCGTGGCGAAACGCCTGAGACACATCACTATTTAATGAAAAAGCCGGGTTATTATc          >  2:784288/1‑140 (MQ=255)                                                                                                                            tCGCCTGGTGCATGCATTGATGCGGATGAATCTCACCTGGGCACAGGTTGGCGGTATTTTAAAATATACCCGTCCGGCGTGGTGGCGTGGCGAAACGCCTGAGACACATCACTATTTAATGAAAAAGCCGGGTTATTATc          >  2:953501/1‑140 (MQ=255)                                                                                                                                    tgcatgcaTTGATGCGGATGAATCTCACCTGGGCACAGGTTGGCGGTATTTTAAAATATACCCGTCCGGCGTGGTGGCGTGGCGAAACGCCTGAGACACATCACTATTTAATGAAAAAGCCGGGTTATTATCTTTCTgaa  <  2:532892/140‑1 (MQ=255)                                                                                                                                    tgcatgcaTTGATGCGGATGAATCTCACCTGGGCACAGGTTGGCGGTATTTTAAAATATACCCGTCCGGCGTGGTGGCGTGGCGAAACGCCTGAGACACATCACTATTTAATGAAAAAGCCGGGTTATTATCTTTCTgaa  <  2:937285/140‑1 (MQ=255)                                                                                                                                    tgcatgcaTTGATGCGGATGAATCTCACCTGGGCACAGGTTGGCGGTATTTTAAAATATACCCGTCCGGCGTGGTGGCGTGGCGAAACGCCTGAGACACATCACTATTTAATGAAAAAGCCGGGTTATTATCTTTCTgaa  <  1:953501/140‑1 (MQ=255)                                                                                                                                         |                                                                                                                                       TCTGACTGACGATCGCTGCAGCGTGGCGGCACTACGTTTACGGGACGGGGAAGAACCGCTTAACGAGCTGCGGCGCAAGATTCGTCAGGACTTATGTCATTTTGAGGGGAATGCACAAGGCATTCGCCTGGTGCATACATTGATGCGGATGAATCTCACCTGGGCACAGGTTGGCGGTATTTTAAAATATACCCGTCCGGCGTGGTGGCGTGGCGAAACGCCTGAGACACATCACTATTTAATGAAAAAGCCGGGTTATTATCTTTCTGAA  >  NC_000913/179683‑179953

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑