Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,666,967	C→T	A85T (GCG→ACG)	ynfK ←	putative dethiobiotin synthetase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,666,967	0	C	T	100.0%	328.4 / NA	91	A85T (GCG→ACG)	ynfK	putative dethiobiotin synthetase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (44/47);  total (44/47)

ATTCAGAGAGTGGACGCAAATCATTCATCAGACTGCGCCAGCCGCCAGTCCCTTCTACCACGACATGATCGACTTTTTCGGTCAGGTTTGCCAGGCCGTTTGAAATGAGGGTGTAATTGATTGGGCAACTGTGCGCCACGCTACTTTCTTCTTCGCTTAACGCGATAGGATTAACTGCTTCATAAGGCAGTTCGATGGTTGAAACACTCTGCAACACCAGGGCATCTTTATTACGCAGCCCTTCGGGTGTCTCTTTGCTCCCCTTCGCTAC  >  NC_000913/1666832‑1667102                                                                                                                                        |                                                                                                                                        aTTCAGAGAGTGGACGCAAATCATTCATCAGACTGCGCCAGCCGCCAGTCCCTTCTACCACGACATGATCGACTTTTTCGGTCAGGTTTGCCAGGCCGTTTGAAATGAGGGTGTAATTGATTGGGCAACTGTGCGTCACg                                                                                                                                     <  1:416182/140‑1 (MQ=255) aTTCAGAGAGTGGACGCAAATCATTCATCAGACTGCGCCAGCCGCCAGTCCCTTCTACCACGACATGATCGACTTTTTCGGTCAGGTTTGCCAGGCCGTTTGAAATGAGGGTGTAATTGATTGGGCAACTGTGCGTCACg                                                                                                                                     >  2:1368714/1‑140 (MQ=255)  ttCAGAGAGTGGACGCAAATCATTCATCAGACTGCGCCAGCCGCCAGTCCCTTCTACCACGACATGATCGACTTTTTCGGTCAGGTTTGCCAGGCCGTTTGAAATGAGGGTGTAATTGATTGGGCAACTGTGCGTCACGc                                                                                                                                    <  2:1368160/140‑1 (MQ=255)      gagagTGGACGCAAATCATTCATCAGACTGCGCCAGCCGCCAGTCCCTTCTACCACGACATGATCGACTTTTTCGGTCAGGTTTGCCAGGCCGTTTGAAATGAGGGTGTAATTGATTGGGCAACTGTGCGTCACGCTACt                                                                                                                                >  1:260980/1‑140 (MQ=255)        gagTGGACGCAAATCATTCATCAGACTGCGCCAGCCGCCAGTCCCTTCTACCACGACATGATCGACTTTTTCGGTCAGGTTTGCCAGGCCGTTTGAAATGAGGGTGTAATTGATTGGGCAACTGTGCGTCACGCTACttt                                                                                                                              >  1:714101/1‑140 (MQ=255)           tGGACGCAAATCATTCATCAGACTGCGCCAGCCGCCAGTCCCTTCTACCACGACATGATCGACTTTTTCGGTCAGGTTTGCCAGGCCGTTTGAAATGAGGGTGTAATTGATTGGGCAACTGTGCGTCACGCTACTttctt                                                                                                                           >  1:25683/1‑140 (MQ=255)           tGGACGCAAATCATTCATCAGACTGCGCCAGCCGCCAGTCCCTTCTACCACGACATGATCGACTTTTTCGGTCAGGTTTGCCAGGCCGTTTGAAATGAGGGTGTAATTGATTGGGCAACTGTGCGTCACGCTACTttctt                                                                                                                           >  2:502148/1‑140 (MQ=255)           tGGACGCAAATCATTCATCAGACTGCGCCAGCCGCCAGTCCCTTCTACCACGACATGATCGACTTTTTCGGTCAGGTTTGCCAGGCCGTTTGAAATGAGGGTGTAATTGATTGGGCAACTGTGCGTCACGCTACTttctt                                                                                                                           >  1:918888/1‑140 (MQ=255)             gACGCAAATCATTCATCAGACTGCGCCAGCCGCCAGTCCCTTCTACCACGACATGATCGACTTTTTCGGTCAGGTTTGCCAGGCCGTTTGAAATGAGGGTGTAATTGATTGGGCAACTGTGCGTCACGCTACTttcttct                                                                                                                         >  2:1410671/1‑140 (MQ=255)             gACGCAAATCATTCATCAGACTGCGCCAGCCGCCAGTCCCTTCTACCACGACATGATCGACTTTTTCGGTCAGGTTTGCCAGGCCGTTTGAAATGAGGGTGTAATTGATTGGGCAACTGTGCGTCACGCTACTttcttct                                                                                                                         >  1:130961/1‑140 (MQ=255)             gACGCAAATCATTCATCAGACTGCGCCAGCCGCCAGTCCCTTCTACCACGACATGATCGACTTTTTCGGTCAGGTTTGCCAGGCCGTTTGAAATGAGGGTGTAATTGATTGGGCAACTGTGCGTCACGCTACTttcttct                                                                                                                         <  1:1368714/140‑1 (MQ=255)             gACGCAAATCATTCATCAGACTGCGCCAGCCGCCAGTCCCTTCTACCACGACATGATCGACTTTTTCGGTCAGGTTTGCCAGGCCGTTTGAAATGAGGGTGTAATTGATTGGGCAACTGTGCGTCACGCTACTttcttct                                                                                                                         >  2:83632/1‑140 (MQ=255)                     tcattcatCAGACTGCGCCAGCCGCCAGTCCCTTCTACCACGACATGATCGACTTTTTCGGTCAGGTTTGCCAGGCCGTTTGAAATGAGGGTGTAATTGATTGGGCAACTGTGCGTCACGCTACTTTCTTCTTCGCTTaa                                                                                                                 <  1:722222/140‑1 (MQ=255)                     tcattcatCAGACTGCGCCAGCCGCCAGTCCCTTCTACCACGACATGATCGACTTTTTCGGTCAGGTTTGCCAGGCCGTTTGAAATGAGGGTGTAATTGATTGGGCAACTGTGCGTCACGCTACTTTCTTCTTCGCTTaa                                                                                                                 >  2:345019/1‑140 (MQ=255)                         tcatcaGACTGCGCCAGCCGCCAGTCCCTTCTACCACGACATGATCGACTTTTTCGGTCAGGTTTGCCAGGCCGTTTGAAATGAGGGTGTAATTGATTGGGCAACTGTGCGTCACGCTACTTTCTTCTTCGCTTAAcgcg                                                                                                             <  2:323970/140‑1 (MQ=255)                         tcatcaGACTGCGCCAGCCGCCAGTCCCTTCTACCACGACATGATCGACTTTTTCGGTCAGGTTTGCCAGGCCGTTTGAAATGAGGGTGTAATTGATTGGGCAACTGTGCGTCACGCTACTTTCTTCTTCGCTTAAcgcg                                                                                                             <  1:1275369/140‑1 (MQ=255)                          catcaGACTGCGCCAGCCGCCAGTCCCTTCTACCACAACATGATCGACTTTTTCGGTCAGGTTTGCCAGGCCGTTTGAAATGAGGGTGTAATTGATTGGGCAACTGTGCGTCACGCTACTTTCTTCTTCGCTTAACGCGa                                                                                                            >  1:112412/1‑140 (MQ=255)                              aGACTGCGCCAGCCGCCAGTCCCTTCTACCACGACATGATCGACTTTTTCGGTCAGGTTTGCCAGGCCGTTTGAAATGAGGGTGTAATTGATTGGGCAACTGTGCGTCACGCTACTTTCTTCTTCGCTTaa                                                                                                                 >  1:852926/1‑131 (MQ=255)                              aGACTGCGCCAGCCGCCAGTCCCTTCTACCACGACATGATCGACTTTTTCGGTCAGGTTTGCCAGGCCGTTTGAAATGAGGGTGTAATTGATTGGGCAACTGTGCGTCACGCTACTTTCTTCTTCGCTTaa                                                                                                                 <  2:852926/131‑1 (MQ=255)                                 cTGCGCCAGCCGCCAGTCCCTTCTACCACGACATGATCGACTTTTTCGGTCAGGTTTGCCAGGCCGTTTGAAATGAGGGTGTAATTGATTGGGCAACTGTGCGTCACGCTACTTTCTTCTTCGCTTAACGCGATAGGAtt                                                                                                     <  2:112412/140‑1 (MQ=255)                                 cTGCGCCAGCCGCCAGTCCCTTCTACCACGACATGATCGACTTTTTCGGTCAGGTTTGCCAGGCCGTTTGAAATGAGGGTGTAATTGATTGGGCAACTGTGCGTCACGCTACTTTCTTCTTCGCTTAACGCGATAGGAtt                                                                                                     <  2:331057/140‑1 (MQ=255)                                  tGCGCCAGCCGCCAGTCCCTTCTACCACGACATGATCGACTTTTTCGGTCAGGTTTGCCAGGCCGTTTGAAATGAGGGTGTAATTGATTGGGCAACTGTGCGTCACGCTACTTTCTTCTTCGCTTAACGCGATAGGATTa                                                                                                    >  2:550475/1‑140 (MQ=255)                                  tGCGCCAGCCGCCAGTCCCTTCTACCACGACATGATCGACTTTTTCGGTCAGGTTTGCCAGGCCGTTTGAAATGAGGGTGTAATTGATTGGGCAACTGTGCGTCACGCTACTTTCTTCTTCGCTTAACGCGATAGGATTa                                                                                                    >  2:165158/1‑140 (MQ=255)                                   gcgcCAGCCGCCAGTCCCTTCTACCACGACATGATCGACTTTTTCGGTCAGGTTTGCCAGGCCGTTTGAAATGAGGGTGTAATTGATTGGGCAACTGTGCGTCACGCTACTTTCTTCTTCGCTTAACGCGATAGGATTaa                                                                                                   >  1:1417204/1‑140 (MQ=255)                                   gcgcCAGCCGCCAGTCCCTTCTACCACGACATGATCGACTTTTTCGGTCAGGTTTGCCAGGCCGTTTGAAATGAGGGTGTAATTGATTGGGCAACTGTGCGTCACGCTACTTTCTTCTTCGCTTAACGCGATAGGATTaa                                                                                                   <  2:1283466/140‑1 (MQ=255)                                   gcgcCAGCCGCCAGTCCCTTCTACCACGACATGATCGACTTTTTCGGTCAGGTTTGCCAGGCCGTTTGAAATGAGGGTGTAATTGATTGGGCAACTGTGCGTCACGCTACTTTCTTCTTCGCTTAACGCGATAGGATTaa                                                                                                   >  2:198959/1‑140 (MQ=255)                                   gcgcCAGCCGCCAGTCCCTTCTACCACGACATGATCGACTTTTTCGGTCAGGTTTGCCAGGCCGTTTGAAATGAGGGTGTAATTGATTGGGCAACTGTGCGTCACGCTACTTTCTTCTTCGCTTAACGCGATAGGATTaa                                                                                                   <  2:419595/140‑1 (MQ=255)                                   gcgcCAGCCGCCAGTCCCTTCTACCACGACATGATCGACTTTTTCGGTCAGGTTTGCCAGGCCGTTTGAAATGAGGGTGTAATTGATTGGGCAACTGTGCGTCACGCTACTTTCTTCTTCGCTTAACGCGATAGGATTaa                                                                                                   <  2:1417901/140‑1 (MQ=255)                                    cgcCAGCCGCCATTCCCTTCTACCACGACATGATCGACTTTTTCGGTCAGGTTTGCCAGGCCGTTTGAAATGAGGGTGTAATTGATTGGGCAACTGTGCGTCACGCTACTTTCTTCTTCGCTTAACGCGATAGGATTAAc                                                                                                  >  1:882092/1‑140 (MQ=255)                                    cgcCAGCCGCCAGTCCCTTCTACCACGACATGATCGACTTTTTCGGTCAGGTTTGCCAGGCCGTTTGAAATGAGGGTGTAATTGATTGGGCAACTGTGCGTCACGCTACTTTCTTCTTCGCTTAACGCGATAGGATTAAc                                                                                                  >  2:521117/1‑140 (MQ=255)                                    cgcCAGCCGCCAGTCCCTTCTACCACGACATGATCGACTTTTTCGGTCAGGTTTGCCAGGCCGTTTGAAATGAGGGTGTAATTGATTGGGCAACTGTGCGTCACGCTACTTTCTTCTTCGCTTAACGCGATAGGATTAAc                                                                                                  >  1:371350/1‑140 (MQ=255)                                    cgcCAGCCGCCAGTCCCTTCTACCACGACATGATCGACTTTTTCGGTCAGGTTTGCCAGGCCGTTTGAAATGAGGGTGTAATTGATTGGGCAACTGTGCGTCACGCTACTTTCTTCTTCGCTTAACGCGATAGGATTAAc                                                                                                  >  2:591430/1‑140 (MQ=255)                                     gcCAGCCGCCAGTCCCTTCTACCACGACATGATCGACTTTTTCGGTCAGGTTTGCCAGGCCGTTTGAAATGAGGGTGTAATTGATTGGGCAACTGTGCGTCACGCTACTTTCTTCTTCGCTTAACGCGATAGGATTAACt                                                                                                 >  2:984271/1‑140 (MQ=255)                                     gcCAGCCGCCAGTCCCTTCTACCACGACATGATCGACTTTTTCGGTCAGGTTTGCCAGGCCGTTTGAAATGAGGGTGTAATTGATTGGGCAACTGTGCGTCACGCTACTTTCTTCTTCGCTTAACGCGATAGGATTAACt                                                                                                 >  1:138166/1‑140 (MQ=255)                                     gcCAGCCGCCAGTCCCTTCTACCACGACATGATCGACTTTTTCGGTCAGGTTTGCCAGGCCGTTTGAAATGAGGGTGTAATTGATTGGGCAACTGTGCGTCACGCTACTTTCTTCTTCGCTTAACGCGATAGGATTAACt                                                                                                 >  2:819842/1‑140 (MQ=255)                                      ccAGCCGCCAGTCCCTTCTACCACGACATGATCGACTTTTTCGGTCAGGTTTGCCAGGCCGTTTGAAATGAGGGTGTAATTGATTGGGCAACTGTGCGTCACGCTACTTTCTTCTTCGCTTAACGCGATAGGATTAACTg                                                                                                <  1:1268983/140‑1 (MQ=255)                                      ccAGCCGCCAGTCCCTTCTACCACGACATGATCGACTTTTTCGGTCAGGTTTGCCAGGCCGTTTGAAATGAGGGTGTAATTGATTGGGCAACTGTGCGTCACGCTACTTTCTTCTTCGCTTAACGCGATAGGATTAACTg                                                                                                <  1:165158/140‑1 (MQ=255)                                          ccgccAGTCCCTTCTACCACGACATGATCGACTTTTTCGGTCAGGTTTGCCAGGCCGTTTGAAATGAGGGTGTAATTGATTGGGCAACTGTGCGTCACGCTACTTTCTTCTTCGCTTAACGCGATAGGAtt                                                                                                     >  2:285993/1‑131 (MQ=255)                                          ccgccAGTCCCTTCTACCACGACATGATCGACTTTTTCGGTCAGGTTTGCCAGGCCGTTTGAAATGAGGGTGTAATTGATTGGGCAACTGTGCGTCACGCTACTTTCTTCTTCGCTTAACGCGATAGGAtt                                                                                                     <  1:285993/131‑1 (MQ=255)                                           cgccAGTCCCTTCTACCACGACATGATCGACTTTTTCGGTCAGGTTTGCCAGGCCGTTTGAAATGAGGGTGTAATTGATTGGGCAACTGTGCGTCACGCTACTTTCTTCTTCGCTTAACGCGATAGGATTAACTGCTTCa                                                                                           <  2:25683/140‑1 (MQ=255)                                                      tCTACCACGACATGATCGACTTTTTCGGTCAGGTTTGCCAGGCCGTTTGAAATGAGGGTGTAATTGATTGGGCAACTGTGCGTCACGCTACTTTCTTCTTCGCTTAACGCGATAGGATTAACTGCTTCATAAGGCAGTTc                                                                                >  2:1071532/1‑140 (MQ=255)                                                       cTACCACGACATGATCGACTTTTTCGGTCAGGTTTGCCAGGCCGTTTGAAATGAGGGTGTAATTGATTGGGCAACTGTGCGTCACGCTACTTTCTTCTTCGCTTAACGCGATAGGATTAACTGCTTCATAAGGCAGTTCg                                                                               <  1:669876/140‑1 (MQ=255)                                                         aCCACGACATGATCGACTTTTTCGGTCAGGTTTGCCAGGCCGTTTGAAATGAGGGTGTAATTGATTGGGCAACTGTGCGTCACGCTACTTTCTTCTTCGCTTAACGCGATAGGATTAACTGCTTCATAAGGCAGTTCGAt                                                                             <  2:672631/140‑1 (MQ=255)                                                         aCCACGACATGATCGACTTTTTCGGTCAGGTTTGCCAGGCCGTTTGAAATGAGGGTGTAATTGATTGGGCAACTGTGCGTCACGCTACTTTCTTCTTCGCTTAACGCGATAGGATTAACTGCTTCATAAGGCAGTTCGAt                                                                             <  2:918888/140‑1 (MQ=255)                                                         aCCACGACATGATCGACTTTTTCGGTCAGGTTTGCCAGGCCGTTTGAAATGAGGGTGTAATTGATTGGGCAACTGTGCGTCACGCTACTTTCTTCTTCGCTTAACGCGATAGGATTAACTGCTTCATAAGGCAGTTCGAt                                                                             <  1:1410671/140‑1 (MQ=255)                                                             cGACATGATCGACTTTTTCGGTCAGGTTTGCCAGGCCGTTTGAAATGAGGGTGTAATTGATTGGGCAACTGTGCGTCACGCTACTTTCTTCTTCGCTTAACGCGATAGGATTAACTGCTTCATAAGGCAGTTCGATGGtt                                                                         >  1:1380567/1‑140 (MQ=255)                                                               aCATGATCGACTTTTTCGGTCAGGTTTGCCAGGCCGTTTGAAATGAGGGTGTAATTGATTGGGCAACTGTGCGTCACGCTACTTTCTTCTTCGCTTAACGCGATAGGATTAACTGCTTCATAAGGCAGTTCGATGGTTGa                                                                       >  2:771733/1‑140 (MQ=255)                                                                cATGATCGACTTTTTCGGTCAGGTTTGCCAGGCCGTTTGAAATGAGGGTGTAATTGATTGGGCAACTGTGCGTCACGCTACTTTCTTCTTCGCTTAACGCGATAGGATTAACTGCTTCATAAGGCAGTTCGATGGTTGaa                                                                      >  2:353146/1‑140 (MQ=255)                                                                  tGATCGACTTTTTCGGTCAGGTTTGCCAGGCCGTTTGAAATGAGGGTGTAATTGATTGGGCAACTGTGCGTCACGCTACTTTCTTCTTCGCTTAACGCGATAGGATTAACTGCTTCATAAGGCAGTTCGATGGTTGAAac                                                                    >  1:1408458/1‑140 (MQ=255)                                                                   gATCGACTTTTTCGGTCAGGTTTGCCAGGCCGTTTGAAATGAGGGTGTAATTGATTGGGCAACTGTGCGTCACGCTACTTTCTTCTTCGCTTAACGCGATAGGATTAACTGCTTCATAAGGCAGTTCGATGGTTGAAaca                                                                   <  2:251580/140‑1 (MQ=255)                                                                    aTCGACTTTTTCGGTCAGGTTTGCCAGGCCGTTTGAAATGAGGGTGTAATTGATTGGGCAACTGTGCGTCACGCTACTTTCTTCTTCGCTTAACGCGATAGGATTAACTGCTTCATAAGGCAGTTCGATGGTTGAAacac                                                                  >  2:570587/1‑140 (MQ=255)                                                                    aTCGACTTTTTCGGTCAGGTTTGCCAGGCCGTTTGAAATGAGGGTGTAATTGATTGGGCAACTGTGCGTCACGCTACTTTCTTCTTCGCTTAACGCGATAGGATTAACTGCTTCATAAGGCAGTTCGATGGTTGAAacac                                                                  <  2:1417204/140‑1 (MQ=255)                                                                         cTTTTTCGGTCAGGTTTGCCAGGCCGTTTGAAATGAGGGTGTAATTGATTGGGCAACTGTGCGTCACGCTACTTTCTTCTTCGCTTAACGCGATAGGATTAACTGCTTCATAAGGCAGTTCGATGGTTTAAACACTCTGc                                                             <  1:83632/140‑1 (MQ=255)                                                                         cTTTTTCGGTCAGGTTTGCCAGGCCGTTTGAAATGAGGGTGTAATTGATTGGGCAACTGTGCGTCACGCTACTTTCTTCTTCGCTTAACGCGATAGGATTAACTGCTTCATAAGGCAGTTCGATGGTTGAAACACTCTGc                                                             <  1:560763/140‑1 (MQ=255)                                                                             ttCGGTCAGGTTTGCCAGGCCGTTTGAAATGAGGGTGTAATTGATTGGGCAACTGTGCGTCACGCTACTTTCTTCTTCGCTTAACGCGATAGGATTAACTGCTTCATAAGGCAGTTCGATGGTTGAAACACTCTGCAaca                                                         <  2:801459/140‑1 (MQ=255)                                                                               cGGTCAGGTTTGCCAGGCCGTTTGAAATGAGGGTGTAATTGATTGGGCAACTGTGCGTCACGCTACTTTCTTCTTCGCTTAACGCGATAGGATTAACTGCTTCATAAGGCAGTTCGATGGTTGAAACACTCTg                                                              <  1:1409970/133‑1 (MQ=255)                                                                               cGGTCAGGTTTGCCAGGCCGTTTGAAATGAGGGTGTAATTGATTGGGCAACTGTGCGTCACGCTACTTTCTTCTTCGCTTAACGCGATAGGATTAACTGCTTCATAAGGCAGTTCGATGGTTGAAACACTCTg                                                              >  2:1409970/1‑133 (MQ=255)                                                                                 gTCAGGTTTGCCAGGCCGTTTGAAATGAGGGTGTAATTGATTGGGCAACTGTGCGTCACGCTACTTTCTTCTTCGCTTAACGCGATAGGATTAACTGCTTCATAAGGCAGTTCGATGGTTGAAACACTCTGCAACACCAg                                                     <  1:502148/140‑1 (MQ=255)                                                                                 gTCAGGTTTGCCAGGCCGTTTGAAATGAGGGTGTAATTGATTGGGCAACTGTGCGTCACGCTACTTTCTTCTTCGCTTAACGCGATAGGATTAACTGCTTCATAAGGCAGTTCGATGGTTGAAACACTCTGCAACACCAg                                                     >  1:136951/1‑140 (MQ=255)                                                                                   cAGGTTTGCCAGGCCGTTTGAAATGAGGGTGTAATTGATTGGGCAACTGTGCGTCACGCTACTTTCTTCTTCGCTTAACGCGATAGGATTAACTGCTTCATAAGGCAGTTCGATGGTTGAAACACTCTGCAACACCAggg                                                   <  1:984271/140‑1 (MQ=255)                                                                                   cAGGTTTGCCAGGCCGTTTGAAATGAGGGTGTAATTGATTGGGCAACTGTGCGTCACGCTACTTTCTTCTTCGCTTAACGCGATAGGATTAACTGCTTCATAAGGCAGTTCGATGGTTGAAACACTCTGCAACACCAggg                                                   <  2:260980/140‑1 (MQ=255)                                                                                   cAGGTTTGCCAGGCCGTTTGAAATGAGGGTGTAATTGATTGGGCAACTGTGCGTCACGCTACTTTCTTCTTCGCTTAACGCGATAGGATTAACTGCTTCATAAGGCAGTTCGATGGTTGAAACACTCTGCAACACCAggg                                                   <  1:819842/140‑1 (MQ=255)                                                                                    aGGTTTGCCAGGCCGTTTGAAATGAGGGTGTAATTGATTGGGCAACTGTGCGTCACGCTACTTTCTTCTTCGCTTAACGCGATAGGATTAACTGCTTCATAAGGCAGTTCGATGGTTGAAACACTCTGCAACACCAGGGc                                                  <  1:932561/140‑1 (MQ=255)                                                                                      gTTTGCCAGGCCGTTTGAAATGAGGGTGTAATTGATTGGGCAACTGTGCGTCACGCTACTTTCTTCTTCGCTTAACGCGATAGGATTAACTGCTTCATAAGGCAGTTCGATGGTTGAAACACTCTGCAACACCAGGGCAt                                                <  2:1408458/140‑1 (MQ=255)                                                                                      gTTTGCCAGGCCGTTTGAAATGAGGGTGTAATTGATTGGGCAACTGTGCGTCACGCTACTTTCTTCTTCGCTTAACGCGATAGGATTAACTGCTTCATAAGGCAGTTCGATGGTTGAAACACTCTGCAACACCAGGGCAt                                                <  1:345019/140‑1 (MQ=255)                                                                                      gTTTGCCAGGCCGTTTGAAATGAGGGTGTAATTGATTGGGCAACTGTGCGTCACGCTACTTTCTTCTTCGCTTAACGCGATAGGATTAACTGCTTCATAAGGCAGTTCGATGGTTGAAACACTCTGCAACACCAGGGCAt                                                <  1:353146/140‑1 (MQ=255)                                                                                               gCCGTTTGAAATGAGGGTGTAATTGATTGGGCAACTGTGCGTCACGCTACTTTCTTCTTCGCTTAACGCGATAGGATTAACTGCTTCATAAGGCAGTTCGATGGTTGAAACACTCTGCAACACCAGGGCATCTTTATTAc                                       >  2:583732/1‑140 (MQ=255)                                                                                                ccGTTTGAAATGAGGGTGTAATTGATTGGGCAACTGTGCGTCACGCTACTTTCTTCTTCGCTTAACGCGATAGGATTAACTGCTTCATAAGGCAGTTCGATGGTTGAAACACTCTGCAACACCAGGGCATCTTTATTAca                                      >  1:230961/1‑139 (MQ=255)                                                                                                 cGTTTGAAATGAGGGTGTAATTGATTGGGCAACTGTGCGTCACGCTACTTTCTTCTTCGCTTAACGCGATAGGATTAACTGCTTCATAAGGCAGTTCGATGGTTGAAACACTCTGCAACACCAGGGCATCTTTATTAcac                                     <  2:230961/140‑3 (MQ=255)                                                                                                     tGAAATGAGGGTGTAATTGATTGGGCAACTGTGCGTCACGCTACTTTCTTCTTCGCTTAACGCGATAGGATTAACTGCTTCATAAGGCAGTTCGATGGTTGAAACACTCTGCAACACCAGGGCATCTTTATTACGCAGcc                                 >  1:202270/1‑140 (MQ=255)                                                                                                       aaaTGAGGGTGTAATTGATTGGGCAACTGTGCGTCACGCTACTTTCTTCTTCGCTTAACGCGATAGGATTAACTGCTTCATAAGGCAGTTCGATGGTTGAAACACTCTGCAACACCAGGGCATCTTTATTACGCAGCCCt                               <  2:1380567/140‑1 (MQ=255)                                                                                                         aTGAGGGTGTAATTGATTGGGCAACTGTGCGTCACGCTACTTTCTTCTTCGCTTAACGCGATAGGATTAACTGCTTCATAAGGCAGTTCGATGGTTGAAACACTCTGCAACACCAGGGCATCTTTATTACGCAGCCCTTc                             <  1:550475/140‑1 (MQ=255)                                                                                                             gggTGTAATTGATTGGGCAACTGTGCGTCACGCTACTTTCTTCTTCGCTTAACGCGATAGGATTAACTGCTTCATAAGGCAGTTCGATGGTTGAAACACTCTGCAACACCAGGGCATCTTTATTACGCAGCCCTTCGGgt                         >  1:1197821/1‑140 (MQ=255)                                                                                                             gggTGTAATTGATTGGGCAACTGTGCGTCACGCTACTTTCTTCTTCGCTTAACGCGATAGGATTAACTGCTTCATAAGGCAGTTCGATGGTTGAAACACTCTGCAACACCAGGGCATCTTTATTACGCAGCCCTTCGGgt                         <  2:1197821/140‑1 (MQ=255)                                                                                                              ggTGTAATTGATTGGGCAACTGTGCGTCACGCTACTTTCTTCTTCGCTTAACGCGATAGGATTAACTGCTTCATAAGGCAGTTCGATGGTTGAAACACTCTGCAACACCAGGGCATCTTTATTACGCAGCCCTTCGGgtg                        <  1:521117/140‑1 (MQ=255)                                                                                                               gtgtAATTGATTGGGCAACTGTGCGTCACGCTACTTTCTTCTTCGCTTAACGCGATAGGATTAACTGCTTCATAAGGCAGTTCGATGGTTGAAACACTCTGCAACACCAGGGCATCTTTATTACGCAGCCCTTCGGgtgt                       <  1:681059/140‑1 (MQ=255)                                                                                                                  tAATTGATTGGGCAACTGTGCGTCACGCTACTTTCTTCTTCGCTTAACGCGATAGGATTAACTGCTTCATAAGGCAGTTCGATGGTTGAAACACTCTGCAACACCAGGGCATCTTTATTACGCAGCCCTTCGGGTGtctc                    >  2:455478/1‑140 (MQ=255)                                                                                                                   aaTTGATTGGGCAACTGTGCGTCACGCTACTTTCTTCTTCGCTTAACGCGATAGGATTAACTGCTTCATAAGGCAGTTCGATGGTTGAAACACTCTGCAACACCAGGGCATCTTTATTACGCAGCCCTTCGGGTGTctct                   <  2:138166/140‑1 (MQ=255)                                                                                                                         ttgGGCAACTGTGCGTCACGCTACTTTCTTCTTCGCTTAACGCGATAGGATTAACTGCTTCATAAGGCAGTTCGATGGTTGAAACACTCTGCAACACCAGGGCATCTTTATTACGCAGCCCTTCGGGTGTCTCTTTGCTc             <  2:1389954/140‑1 (MQ=255)                                                                                                                          tgGGCAACTGTGCGTCACGCTACTTTCTTCTTCGCTTAACGCGATAGGATTAACTGCTTCATAAGGCAGTTCGATGGTTGAAACACTCTGCAACACCAGGGCATCTTTATTACGCAGCCCTTCGGGTGTCTCTTTGCTcc            >  1:25632/1‑140 (MQ=255)                                                                                                                            ggCAACTGTGCGTCACGCTACTTTCTTCTTCGCTTAACGCGATAGGATTAACTGCTTCATAAGGCAGTTCGATGGTTGAAACACTCTGCAACACCAGGGCATCTTTATTACGCAGCCCTTCGGGTGtct                     <  2:1413853/129‑1 (MQ=255)                                                                                                                            ggCAACTGTGCGTCACGCTACTTTCTTCTTCGCTTAACGCGATAGGATTAACTGCTTCATAAGGCAGTTCGATGGTTGAAACACTCTGCAACACCAGGGCATCTTTATTACGCAGCCCTTCGGGTGtct                     <  1:1004512/129‑1 (MQ=255)                                                                                                                            ggCAACTGTGCGTCACGCTACTTTCTTCTTCGCTTAACGCGATAGGATTAACTGCTTCATAAGGCAGTTCGATGGTTGAAACACTCTGCAACACCAGGGCATCTTTATTACGCAGCCCTTCGGGTGtct                     >  2:1004512/1‑129 (MQ=255)                                                                                                                            ggCAACTGTGCGTCACGCTACTTTCTTCTTCGCTTAACGCGATAGGATTAACTGCTTCATAAGGCAGTTCGATGGTTGAAACACTCTGCAACACCAGGGCATCTTTATTACGCAGCCCTTCGGGTGtct                     >  1:1413853/1‑129 (MQ=255)                                                                                                                             gCAACTGTGCGTCACGCTACTTTCTTCTTCGCTTAACGCGATAGGATTAACTGCTTCATAAGGCAGTTCGATGGTTGAAACACTCTGCAACACCAGGGCATCTTTATTACGCAGCCCTTCGGGTGTCTCTTTGCTCCCCt         <  1:455478/140‑1 (MQ=255)                                                                                                                              cAACTGTGCGTCACGCTACTTTCTTCTTCGCTTAACGCGATAGGATTAACTGCTTCATAAGGCAGTTCGATGGTTGAAACACTCTGCAACACCAGGGCATCTTTATTACGCAGCCCTTCGGGTGTCTCTTTGCTCCCCtt        <  2:882092/140‑1 (MQ=255)                                                                                                                                  tgtgCGTCACGCTACTTTCTTCTTCGCTTAACGCGATAGGATTAACTGCTTCATAAGGCAGTTCGATGGTTGAAACACTCTGCAACACCAGGGCATCTTTATTACGCAGCCCTTCGGGTGTCTCTTTGCTCCCCTTCGCt    >  1:395714/1‑140 (MQ=255)                                                                                                                                  tgtgCGTCACGCTACTTTCTTCTTCGCTTAACGCGATAGGATTAACTGCTTCATAAGGCAGTTCGATGGTTGAAACACTCTGCAACACCAGGGCATCTTTATTACGCAGCCCTTCGGGTGTCTCTTTGCTCCCCTTCGCt    >  1:1058329/1‑140 (MQ=255)                                                                                                                                   gtgCGTCACGCTACTTTCTTCTTCGCTTAACGCGATAGGATTAACTGCTTCATAAGGCAGTTCGATGGTTGAAACACTCTGCAACACCAGGGCATCTTTATTACGCAGCCCTTCGGGTGTCTCTTTGCTCCCCTTCGCTa   <  1:570587/140‑1 (MQ=255)                                                                                                                                    tgCGTCACGCTACTTTCTTCTTCGCTTAACGCGATAGGATTAACTGCTTCATAAGGCAGTTCGATGGTTGAAACACTCTGCAACACCAGGGCATCTTTATTACGCAGCCCTTCGGGTGTCTCTTTGCTCCCCTTCGCTAc  >  1:841216/1‑140 (MQ=255)                                                                                                                                    tgCGTCACGCTACTTTCTTCTTCGCTTAACGCGATAGGATTAACTGCTTCATAAGGCAGTTCGATGGTTGAAACACTCTGCAACACCAGGGCATCTTTATTACGCAGCCCTTCGGGTGTCTCTTTGCTCCCCTTCGCTAc  >  1:1100437/1‑140 (MQ=255)                                                                                                                                        |                                                                                                                                        ATTCAGAGAGTGGACGCAAATCATTCATCAGACTGCGCCAGCCGCCAGTCCCTTCTACCACGACATGATCGACTTTTTCGGTCAGGTTTGCCAGGCCGTTTGAAATGAGGGTGTAATTGATTGGGCAACTGTGCGCCACGCTACTTTCTTCTTCGCTTAACGCGATAGGATTAACTGCTTCATAAGGCAGTTCGATGGTTGAAACACTCTGCAACACCAGGGCATCTTTATTACGCAGCCCTTCGGGTGTCTCTTTGCTCCCCTTCGCTAC  >  NC_000913/1666832‑1667102

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑