Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,810,965	T→C	V11A (GTG→GCG)	tcyP →	cystine/sulfocysteine:cation symporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,810,965	0	T	C	100.0%	115.1 / NA	32	V11A (GTG→GCG)	tcyP	cystine/sulfocysteine:cation symporter
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (26/6);  total (26/6)

ATAAGCAATTCCATTTGAATATAAGAGCCAGCTCACAGTTCTGTTAATCTTGCGCCAACACTATGACTGCTACGCAGTGATAGAAATAATAAGATCAGGAGAACGGGGATGAACTTTCCATTAATTGCGAACATCGTGGTGTTCGTTGTACTGCTGTTTGCGCTGGCTCAGACCCGCCATAAACAGTGGAGTCTGGCGAAAAAAGTGCTGGTGGGTCTGGTGATGGGTGTGGTTTTTGGCCTTGCCCTGCATACCATTTATGGTTCTGA  >  NC_000913/1810826‑1811094                                                                                                                                            |                                                                                                                                  aTAAGCAATTCCATTTGAATATAAGAGCCAGCTCACAGTTCTGTTAATCTTGCGCCAACACTATGACTGCTACGCAGTGATAGAAATAATAAGATCAGGAGAACGGGGATGAACTTTCCATTAATTGCGAACATCgtggt                                                                                                                                   <  1:659446/140‑1 (MQ=255)    aGCAATTCCATTTGAATATAAGAGCCAGCTCACAGTTCTGTTAATCTTGCGCCAACACTATGACTGCTACGCAGTGATAGAAATAATAAGATCAGGAGAACGGGGATGAACTTTCCATTAATTGCGAACATCGTGGCGtt                                                                                                                                >  1:1277599/1‑140 (MQ=255)      cAATTCCATTTGAATATAAGAGCCAGCTCACAGTTCTGTTAATCTTGCGCCAACACTATGACTGCTACGCAGTGATAGAAATAATAAGATCAGGAGAACGGGGATGAACTTTCCATTAATTGCGAACATCGTGGCGTTCg                                                                                                                              <  2:916974/140‑1 (MQ=255)      cAATTCCATTTGAATATAAGAGCCAGCTCACAGTTCTGTTAATCTTGCGCCAACACTATGACTGCTACGCAGTGATAGAAATAATAAGATCAGGAGAACGGGGATGAACTTTCCATTAATTGCGAACATCGTGGCGTTCg                                                                                                                              <  2:426845/140‑1 (MQ=255)              tttGAATATAAGAGCCAGCTCACAGTTCTGTTAATCTTGCGCCAACACTATGACTGCTACGCAGTGATAGAAATAATAAGATCAGGAGAACGGGGATGAACTTTCCATTAATTGCGAACATCGTGGCGTTCGTTGTActg                                                                                                                      >  1:1154518/1‑140 (MQ=255)              tttGAATATAAGAGCCAGCTCACAGTTCTGTTAATCTTGCGCCAACACTATGACTGCTACGCAGTGATAGAAATAATAAGATCAGGAGAACGGGGATGAACTTTCCATTAATTGCGAACATCGTGGCGTTCGTTGTActg                                                                                                                      >  1:741704/1‑140 (MQ=255)              tttGAATATAAGAGCCAGCTCACAGTTCTGTTAATCTTGCGCCAACACTATGACTGCTACGCAGTGATAGAAATAATAAGATCAGGAGAACGGGGATGAACTTTCCATTAATTGCGAACATCGTGGCGTTCGTTGTActg                                                                                                                      >  1:1353760/1‑140 (MQ=255)                       aaGAGCCAGCTCACAGTTCTGTTAATCTTGCGCCAACACTATGACTGCTACGCAGTGATAGAAATAATAAGATCAGGAGAACGGGGATGAACTTTCCATTAATTGCGAACATCGTGGCGTTCGTTGTACTGCTGTTTgcg                                                                                                             <  1:1041423/140‑1 (MQ=255)                       aaGAGCCAGCTCACAGTTCTGTTAATCTTGCGCCAACACTATGACTGCTACGCAGTGATAGAAATAATAAGATCAGGAGAACGGGGATGAACTTTCCATTAATTGCGAACATCGTGGCGTTCGTTGTACTGCTGTTTgcg                                                                                                             <  2:741704/140‑1 (MQ=255)                       aaGAGCCAGCTCACAGTTCTGTTAATCTTGCGCCAACACTATGACTGCTACGCAGTGATAGAAATAATAAGATCAGGAGAACGGGGATGAACTTTCCATTAATTGCGAACATCGTGGCGTTCGTTGTACTGCTGTTTgcg                                                                                                             <  2:1277599/140‑1 (MQ=255)                       aaGAGCCAGCTCACAGTTCTGTTAATCTTGCGCCAACACTATGACTGCTACGCAGTGATAGAAATAATAAGATCAGGAGAACGGGGATGAACTTTCCATTAATTGCGAACATCGTGGCGTTCGTTGTACTGCTGTTTgcg                                                                                                             <  2:1397400/140‑1 (MQ=255)                                cTCACAGTTCTGTTAATCTTGCGCCAACACTATGACTGCTACGCAGTGATAGAAATAATAAGATCAGGAGAACGGGGATGAACTTTCCATTAATTGCGAACATCGTGGCGTTCGTTGTACTGCTGTTTGCGCTGGCTCAg                                                                                                    >  2:497992/1‑140 (MQ=255)                                        tCTGTTAATCTTGCGCCAACACTATGACTGCTACGCAGTGATAGAAATAATAAGATCAGGAGAACGGGGATGAACTTTCCATTAATTGCGAACATCGTGGCGTTCGTTGTACTGCTGTTTGCGCTGGCTCAGACCCGCCa                                                                                            >  1:1318382/1‑140 (MQ=255)                                          tttaattCTAGCCCCAACACTATGACTGCTACGAAGTAAAAAAAAAAAAAAAATGAGGAGAGGGGGGTTGACTTTTCAATAAATTGCAAACATCGGGGCTTTCTTTGTATTGTTTTTTGCGCTGGCTAAGACCCGCCATa                                                                                          <  2:31369/134‑1 (MQ=255)                                            ttAATCTTGCGCCAACACTATGACTGCTACGCAGTGATAGAAATAATAAGATCAGGAGAACGGGGATGAACTTTCCATTAATTGCGAACATCGTGGCGTTCGCTGTACTGCTGTTTGCGCTGGCTCAGACCCGCCATaaa                                                                                        >  1:809598/1‑140 (MQ=255)                                                 cTTGCGCCAACACTATGACTGCTACGCAGTGATAGAAATAATAAGATCAGGAGAACGGGGATGAACTTTCCATTAATTGCGAACATCGTGGCGTTCGTTGTACTGCTGTTTGCGCTGGCTCAGACCCGCCATAAACAGTg                                                                                   >  2:1063130/1‑140 (MQ=255)                                                    gcgcCAACACTATGACTGCTACGCAGTGATAGAAATAATAAGATCAGGAGAACGGGGATGAACTTTCCATTAATTGCGAACATCGTGGCGTTCGTTGTACTGCTGTTTGCGCTGGCTCAGACCCGCCATAAACAGTGGAg                                                                                >  1:392546/1‑140 (MQ=255)                                                                tGACTGCTACGCAGTGATAGAAATAATAAGATCAGGAGAACGGGGATGAACTTTCCATTAATTGCGAACATCGTGGCGTTCGTTGTACTGCTGTTTGCGCTGGCTCAGACCCGCCATAAACAGTGGAGTCTGGCGaaaaa                                                                    >  1:1143406/1‑140 (MQ=255)                                                                     gCTACGCAGTGATAGAAATAATAAGATCAGGAGAACGGGGATGAACTTTCCATTAATTGCGAACATCGTGGCGTTCGTTGTACTGCTGTTTGCGCTGGCTCAGACCCGCCATAAACAGTGGAGTCTGGCGAAAAAAGTGc                                                               >  1:302926/1‑140 (MQ=255)                                                                      cTACGCAGTGATAGAAATAATAAGATCAGGAGAACGGGGATGAACTTTCCATTAATTGCGAACATCGTGGCGTTCGTTGTACTGCTGTTTGCGCTGGCTCAGACCCGCCATAAACAGTGGAGTCTGGCGAAAAAAGTGCt                                                              >  1:875066/1‑140 (MQ=255)                                                                      cTACGCAGTGATAGAAATAATAAGATCAGGAGAACGGGGATGAACTTTCCATTAATTGCGAACATCGTGGCGTTCGTTGTACTGCTGTTTGCGCTGGCTCAGACCCGCCATAAACAGTGGAGTCTGGCGAAAAAAGTGCt                                                              >  2:569684/1‑140 (MQ=255)                                                                      cTACGCAGTGATAGAAATAATAAGATCAGGAGAACGGGGATGAACTTTCCATTAATTGCGAACATCGTGGCGTTCGTTGTACTGCTGTTTGCGCTGGCTCAGACCCGCCATAAACAGTGGAGTCTGGCGAAAAAAGTGCt                                                              >  1:967325/1‑140 (MQ=255)                                                                      cTACGCAGTGATAGAAATAATAAGATCAGGAGAACGGGGATGAACTTTCCATTAATTGCGAACATCGTGGCGTTCGTTGTACTGCTGTTTGCGCTGGCTCAGACCCGCCATAAACAGTGGAGTCTGGCGAAAAAAGTGCt                                                              >  1:1164114/1‑140 (MQ=255)                                                                              tGATAGAAATAATAAGATCAGGAGAACGGGGATGAACTTTCCATTAATTGCGAACATCGTGGCGTTCGTTGTACTGCTGTTTGCGCTGGCTCAGACCCGCCATAAACAGTGGAGTCTGGCGAAAAAAGTGCTGGTGGGTc                                                      >  2:659285/1‑140 (MQ=255)                                                                              tGATAGAAATAATAAGATCAGGAGAACGGGGATGAACTTTCCATTAATTGCGAACATCGTGGCGTTCGTTGTACTGCTGTTTGCGCTGGCTCAGACCCGCCATAAACAGTGGAGTCTGGCGAAAAAAGTGCTGGTGGGTc                                                      >  2:237270/1‑140 (MQ=255)                                                                                   aaaaaaaaaAGAGCAGGGGAGGGGGGATAATTTTTCAATTAATGGCAAACAGGGTGGGTTTGTTTTTACTGTTTTTGGCGCGGCCCCAGACCCGCCATAAACAGTGGAGTCTGGCGAAAAAAGTGCTGGTGGGTCTGGTg                                                 <  2:122495/139‑1 (MQ=255)                                                                                        aataaGATCAGGAGAACGGGGATGAACTTTCCATTAATTGCGAACATCGTGGCGTTCGTTGTACTGCTGTTTGCGCTGGCTCAGACCCGCCATAAACAGTGGAGTCTGGCGAAAAAAGTGCTGGTGGGTCTggctgtctc                                            >  1:177533/1‑133 (MQ=255)                                                                                        aataaGATCAGGAGAACGGGGATGAACTTTCCATTAATTGCGAACATCGTGGCGTTCGTTGTACTGCTGTTTGCGCTGGCTCAGACCCGCCATAAACAGTGGAGTCTGGCGAAAAAAGTGCTGGTGGGTCTGGTGATggg                                            >  1:1190601/1‑140 (MQ=255)                                                                                         ataaGATCAGGAGAACGGGGATGAACTTTCCATTAATTGCGAACATCGTGGCGTTCGTTGTACTGCTGTTTGCGCTGGCTCAGACCCGCCATAAACAGTGGAGTCTGGCGAAAAAAGTGCTGGTGGGTCTGGTGATGGgt                                           >  1:1398211/1‑140 (MQ=255)                                                                                              aTCAGGAGAACGGGGATGAACTTTCCATTAATTGCGAACATCGTGGCGTTCGTTGTACTGCTGTTTGCGCTGGCTCAGACCCGCCATAAACAGTGGAGTCTGGCGAAAAAAGTGCTGGTGGGTCTGGTGATGGGTGTGGt                                      >  1:78966/1‑140 (MQ=255)                                                                                                cAGGAGAACGGGGATGAACTTTCCATTAATTGCGAACATCGTGGCGTTCGTTGTACTGCTGTTTGCGCTGGCTCAGACCCGCCATAAACAGTGGAGTCTGGCGAAAAAAGTGCTGGTGGGTCTGGTGATGGGTGTGGttt                                    >  2:659675/1‑140 (MQ=255)                                                                                                     gaACGGGGATGAACTTTCCATTAATTGCGAACATCGTGGCGTTCGTTGTACTGCTGTTTGCGCTGGCTCAGACCCGCCATAAACAGTGGAGTCTGGCGAAAAAAGTGCTGGTGGGTCTGGTGATGGGTGTGGTTTTTGGc                               >  1:912626/1‑140 (MQ=255)                                                                                                                atttttCAATTAATTGCGACCGTGGGGGCGTTCTTTTTCCTGTTGTTGGCGCGGGCCCAGCCCCGCCATAAACAGTGGAGTCTGGCGAAAAAAGTGCTGGTGGGTCTGGTGATGGGTGTGGTTTTTGGCCTTGCCCTGCa                    <  2:912626/137‑1 (MQ=255)                                                                                                                    ttataTTAATTGCGAAGGTGGGGGCGTTGTTTTTCTTGTTGTTGGCGTTCGCCCACCCCCGCCATAAACAGTGGAGTCTGGCGAAAAAAGTGCTGGTGGGTCTGGTGATGGGTGTGGTTTTTGGCCTTGCCCTGCATAcc                <  1:1063130/136‑1 (MQ=255)                                                                                                                     tCCATTAATTGCGAACATCGTGGCGTTCGTTGTACTGCTGTTTGCGCTGGCTCAGACCCGCCATAAACAGTGGAGTCTGGCGAAAAAAGTGCTGGTGGGTCTGGTGATGGGTGTGGTTTTTGGCCTTGCCCTGCATACCa               >  1:205629/1‑140 (MQ=255)                                                                                                                     tCCATTAATTGCGAACATCGTGGCGTTCGTTGTACTGCTGTTTGCGCTGGCTCAGACCCGCCATAAACAGTGGAGTCTGGCGAAAAAAGTGCTGGTGGGTCTGGTGATGGGTGTGGTTTTTGGCCTTGCCCTGCATACCa               >  1:22542/1‑140 (MQ=255)                                                                                                                     tCCATTAATTGCGAACATCGTGGCGTTCGTTGTACTGCTGTTTGCGCTGGCTCAGACCCGCCATAAACAGTGGAGTCTGGCGAAAAAAGTGCTGGTGGGTCTGGTGATGGGTGTGGTTTTTGGCCTTGCCCTGCATACCa               >  1:721951/1‑140 (MQ=255)                                                                                                                        tttAATTGCGAACGTGGTGGCTTTTGTTGTCTTGTTGTTGGCGCTGGCCCAGCCCCGCCATAAACAGTGGAGTCTGGCGAAAAAAGTGCTGGTGGGTCTGGTGATGGGTGTGGTTTTTGGCCTTGCCCTGCATACCAttt            <  2:1190601/139‑1 (MQ=255)                                                                                                                        ttaaagtGCGAATCTGGTGGTTTTTTTTGTTTTGGTGTTTGGGGTGCCGCAGCCCCGCCATAAACAGTGGAGTCTGGCGAAAAAAGTGCTGGTGGGTCTGGTGATGGGTGTGGTTTTTGGCCTTGCCCTGCATACCAttt            <  2:392546/134‑1 (MQ=255)                                                                                                                                  aagtgcGTGGTTTTTTTTGTCCTGTTTTTTGCGCGGCCCCAGCCCCGCCATAAACAGTGGAGTCTGGCGAAAAAAGTGCTGGTGGGTCTGGTGATGGGTGTGGTTTTTGGCCTTGCCCTGCATACCATTTATGGTTCTGa  <  2:427551/135‑1 (MQ=255)                                                                                                                                            |                                                                                                                                  ATAAGCAATTCCATTTGAATATAAGAGCCAGCTCACAGTTCTGTTAATCTTGCGCCAACACTATGACTGCTACGCAGTGATAGAAATAATAAGATCAGGAGAACGGGGATGAACTTTCCATTAATTGCGAACATCGTGGTGTTCGTTGTACTGCTGTTTGCGCTGGCTCAGACCCGCCATAAACAGTGGAGTCTGGCGAAAAAAGTGCTGGTGGGTCTGGTGATGGGTGTGGTTTTTGGCCTTGCCCTGCATACCATTTATGGTTCTGA  >  NC_000913/1810826‑1811094

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑