Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,067,537	T→C	intergenic (‑216/‑1098)	insH‑6 ← / → yoeA	CP4‑44 prophage; IS5 transposase and trans‑activator/CP4‑44 prophage; TonB‑dependent receptor plug domain‑containing protein YoeA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,067,537	0	T	C	100.0%	277.9 / NA	75	intergenic (‑216/‑1098)	insH‑6/yoeA	CP4‑44 prophage; IS5 transposase and trans‑activator/CP4‑44 prophage; TonB‑dependent receptor plug domain‑containing protein YoeA
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (37/38);  total (37/38)

CAACAGAATGTGACTTGCCAGATTTATTAGCGCCACCAGATGTTTAACCGGGTAACCACGACCCAGTCGGGGATATATTCCATCTTCTCGCTGACATCATCAAGAATAAAAAGGTTTATCACACTCAGAGCAACGTTCTTCAGCAGGCGGGATAATTCTTCAACATTTAGAAGATGTGTTATTAAAGGCATACAAACTGGACGTATTGTATTTTCTTTTGGTGGTTGACCTAAGGTAGCAGTTTATCCTGATGCGCTGAGATTTCTGT  >  NC_000913/2067402‑2067669                                                                                                                                        |                                                                                                                                     cAACAGAATGTGACTTGCCAGATTTATTAGCGCCACCAGATGTTTAACCGGGTAACCACGACCCAGTCGGGGATATATTCCATCTTCTCGCTGACATCATCAAGAATAAAAAGGTTTATCACACTCAGAGCAACGctctt                                                                                                                                  >  2:725709/1‑140 (MQ=255)     aGAATGTGACTTGCCAGATTTATTAGCGCCACCAGATGTTTAACCGGGTAACCACGACCCAGTCGGGGATATATTCCATCTTCTCGCTGACATCATCAAGAATAAAAAGGTTTATCACACTCAGAGCAACGCTCTTcagc                                                                                                                              <  2:326824/140‑1 (MQ=255)        aTGTGACTTGCCAGATTTATTAGCGCCACCAGATGTTTAACCGGGTAACCACGACCCAGTCGGGGATATATTCCATCTTCTCGCTGACATCATCAAGAATAAAAAGGTTTATCACACTCAGAGCAACGCTCTTCAGCAgg                                                                                                                           <  2:1195293/140‑1 (MQ=255)         tgtgACTTGCCAGATTTATTAGCGCCACCAGATGTTTAACCGGGTAACCACGACCCAGTCGGGGATATATTCCATCTTCTCGCTGACATCATCAAGAATAAAAAGGTTTATCACACTCAGAGCAACGCTCTTcagca                                                                                                                             >  2:1394520/1‑137 (MQ=255)         tgtgACTTGCCAGATTTATTAGCGCCACCAGATGTTTAACCGGGTAACCACGACCCAGTCGGGGATATATTCCATCTTCTCGCTGACATCATCAAGAATAAAAAGGTTTATCACACTCAGAGCAACGCTCTTcagca                                                                                                                             <  1:1394520/137‑1 (MQ=255)         tgtgACTTGCCAGATTTATTAGCGCCACCAGATGTTTAACCGGGTAACCACGACCCAGTCGGGGATATATTCCATCTTCTCGCTGACATCATCAAGAATAAAAAGGTTTATCACACTCAGAGCAACGCTCTTCAGCAGGc                                                                                                                          >  2:294262/1‑140 (MQ=255)              cTTGCCAGTTTTATTAGCGCCACCAGATGTTTAACCGGGTAACCACGACCCAGTCGGGGATATATTCCATCTGCTCGCTGACATCATGAAGAATAAAAAGGTTTATCACACTCAGAGCAACGCTCTTCAGCAGGCGGGAt                                                                                                                     <  2:876761/140‑1 (MQ=255)              cTTGCCAGATTTATTAGCGCCACCAGATGTTTAACCGGGTAACCACGACCCAGTCGGGGATATATTCCATCTTCTCGCTGACATCATCAAGAATAAAAAGGTTTATCACACTCAGAGCAACGCTCTTCAGCAGGCGGGAt                                                                                                                     <  1:725709/140‑1 (MQ=255)              cTTGCCAGATTTATTAGCGCCACCAGATGTTTAACCGGGTAACCACGACCCAGTCGGGGATATATTCCATCTTCTCGCTGACATCATCAAGAATAAAAAGGTTTATCACACTCAGAGCAACGCTCTTCAGCAGGCGGGAt                                                                                                                     <  1:57031/140‑1 (MQ=255)                 gCCAGATTTATTAGCGCCACCAGATGTTTAACCGGGTAACCACGACCCAGTCGGGGATATATTCCATCTTCTCGCTGACATCATCAAGAATAAAAAGGTTTATCACACTCAGAGCAACGCTCTTCAGCAGGCGGGATAAt                                                                                                                  >  2:594230/1‑140 (MQ=255)                 gCCAGATTTATTAGCGCCACCAGATGTTTAACCGGGTAACCACGACCCAGTCGGGGATATATTCCATCTTCTCGCTGACATCATCAAGAATAAAAAGGTTTATCACACTCAGAGCAACGCTCTTCAGCAGGCGGGATAAt                                                                                                                  >  1:644564/1‑140 (MQ=255)                     gATTTATTAGCGCCACCAGATGTTTAACCGGGTAACCACGACCCAGTCGGGGATATATTCCATCTTCTCGCTGACATCATCAAGAATAAAAAGGTTTATCACACTCAGAGCAACGCTCTTCAGCAGGCGGGATAAttctt                                                                                                              <  2:934922/140‑1 (MQ=255)                     gATTTATTAGCGCCACCAGATGTTTAACCGGGTAACCACGACCCAGTCGGGGATATATTCCATCTTCTCGCTGACATCATCAAGAATAAAAAGGTTTATCACACTCAGAGCAACGCTCTTCAGCAGGCGGGATAAttctt                                                                                                              <  2:195304/140‑1 (MQ=255)                       tttATTAGCGCCACCAGATGTTTAACCGGGTAACCACGACCCAGTCGGGGATATATTCCATCTTCTCGCTGACATCATCAAGAATAAAAAGGTTTATCACACTCAGAGCAACGCTCTTCAGCAGGCGGGATAATTCTTCa                                                                                                            <  1:764491/140‑1 (MQ=255)                          attaGCGCCACCAGATGTTTAACCGGGTAACCACGACCCAGTCGGGGATATATTCCATCTTCTCGCTGACATCATCAAGAATAAAAAGGTTTATCACACTCAGAGCAACGCTCTTCAGCAGGCGGGATAATTCTTCAACa                                                                                                         <  2:770946/140‑1 (MQ=255)                            taGCGCCACCAGATGTTTAACCGGGTAACCACGACCCAGTCGGGGATATATTCCATCTTCTCGCTGACATCATCAAGAATAAAAAGGTTTATCACACTCAGAGCAACGCTCTTCAGCAGGCGGGATAATTCTTCAACAtt                                                                                                       <  1:1299155/140‑1 (MQ=255)                               cgcCACCAGATGTTTAACCGGGTAACCACGACCCAGTCGGGGATATATTCCATCTTCTCGCTGACATCATCAAGAATAAAAAGGTTTATCACACTCAGAGCAACGCTCTTCAGCAGGCGGGATAATTCTTCAACATTTag                                                                                                    >  1:1368570/1‑140 (MQ=255)                               cgcCACCAGATGTTTAACCGGGTAACCACGACCCAGTCGGGGATATATTCCATCTTCTCGCTGACATCATCAAGAATAAAAAGGTTTATCACACTCAGAGCAACGCTCTTCAGCAGGCGGGATAATTCTTCAACATTTag                                                                                                    <  2:356959/140‑1 (MQ=255)                                gcCACCAGATGTTTAACCGGGTAACCACGACCCAGTCGGGGATATATTCCATCTTCTCGCTGACATCATCAAGAATAAAAAGGTTTATCACACTCAGAGCAACGCTCTTCAGCAGGCGGGATAAttcttc                                                                                                             >  1:98232/1‑130 (MQ=255)                                gcCACCAGATGTTTAACCGGGTAACCACGACCCAGTCGGGGATATATTCCATCTTCTCGCTGACATCATCAAGAATAAAAAGGTTTATCACACTCAGAGCAACGCTCTTCAGCAGGCGGGATAAttcttc                                                                                                             <  2:98232/130‑1 (MQ=255)                                  caccaGATGTTTAACCGGGTAACCACGACCCAGTCGGGGATATATTCCATCTTCTCGCTGACATCATCAAGAATAAAAAGGTTTATCACACTCAGAGCAACGCTCTTCAGCAGGCGGGATAATTCTTCAACATTTagaag                                                                                                 <  1:202359/140‑1 (MQ=255)                                  caccaGATGTTTAACCGGGTAACCACGACCCAGTCGGGGATATATTCCATCTTCTCGCTGACATCATCAAGAATAAAAAGGTTTATCACACTCAGAGCAACGCTCTTCAGCAGGCGGGATAATTCTTCAACATTTagaag                                                                                                 <  1:594230/140‑1 (MQ=255)                                             tAACCGGGTAACCACGACCCAGTCGGGGATATATTCCATCTTCTCGCTGACATCATCAAGAATAAAAAGGTTTATCACACTCAGAGCAACGCTCTTCAGCAGGCGGGATAATTCTTCAACATTTAGAAGATGTGttatta                                                                                      >  1:254587/1‑140 (MQ=255)                                             tAACCGGGTAACCACGACCCAGTCGGGGATATATTCCATCTTCTCGCTGACATCATCAAGAATAAAAAGGTTTATCACACTCAGAGCAACGCTCTTCAGCAGGCGGGATAATTCTTCAACATTTAGAAGATGTGttatta                                                                                      <  1:592217/140‑1 (MQ=255)                                                 cGGGTAACCACGACCCAGTCGGGGATATATTCCATCTTCTCGCTGACATCATCAAGAATAAAAAGGTTTATCACACTCAGAGCAACGCTCTTCAGCAGGCGGGATAATTCTTCAACATTTAGAAGAt                                                                                               <  2:771585/127‑1 (MQ=255)                                                 cGGGTAACCACGACCCAGTCGGGGATATATTCCATCTTCTCGCTGACATCATCAAGAATAAAAAGGTTTATCACACTCAGAGCAACGCTCTTCAGCAGGCGGGATAATTCTTCAACATTTAGAAGAt                                                                                               >  1:771585/1‑127 (MQ=255)                                                  gggTAACCACGACCCAGTCGGGGATATATTCCATCTTCTCGCTGACATCATCAAGAATAAAAAGGTTTATCACACTCAGAGCAACGCTCTTCAGCAGGCGGGATAATTCTTCAACATTTAGAAGAtgtg                                                                                            <  2:839714/129‑1 (MQ=255)                                                  gggTAACCACGACCCAGTCGGGGATATATTCCATCTTCTCGCTGACATCATCAAGAATAAAAAGGTTTATCACACTCAGAGCAACGCTCTTCAGCAGGCGGGATAATTCTTCAACATTTAGAAGAtgtg                                                                                            >  1:839714/1‑129 (MQ=255)                                                   ggTAACCACGACCCAGTCGGGGATATATTCCATCTTCTCGCTGACATCATCAAGAATAAAAAGGTTTATCACACTCAGAGCAACGCTCTTCAGCAGGCGGGATAATTCTTCAACATTTAGAAGATGTGTTATTAAAGGCa                                                                                >  2:356495/1‑140 (MQ=255)                                                       aCCACGACCCAGTCGGGGATATATTCCATCTTCTCGCTGACATCATCAAGAATAAAAAGGTTTATCACACTCAGAGCAACGCTCTTCAGCAGGCGGGATAATTCTTCAACATTTAGAAGATGTGTTATTAAAGGCATACa                                                                            >  1:180113/1‑140 (MQ=255)                                                         cACGACCCAGTCGGGGATATATTCCATCTTCTCGCTGACATCATCAAGAATAAAAAGGTTTATCACACTCAGAGCAACGCTCTTCAGCAGGCGGGATAATTCTTCAACATTTAGAAGATGTGTTATTAAAGGCATACaaa                                                                          >  1:268714/1‑140 (MQ=255)                                                          aCGACCCAGTCGGGGATATATTCCATCTTCTCGCTGACATCATCAAGAATAAAAAGGTTTATCACACTCAGAGCAACGCTCTTCAGCAGGCGGGATAATTCTTCAACATTTAGAAGATGTGTTATTAAAGGCATACAAAc                                                                         <  2:1405139/140‑1 (MQ=255)                                                          aCGACCCAGTCGGGGATATATTCCATCTTCTCGCTGACATCATCAAGAATAAAAAGGTTTATCACACTCAGAGCAACGCTCTTCAGCAGGCGGGATAATTCTTCAACATTTAGAAGATGTGTTATTAAAGGCATACAAAc                                                                         >  2:341462/1‑140 (MQ=255)                                                          aCGACCCAGTCGGGGATATATTCCATCTTCTCGCTGACATCATCAAGAATAAAAAGGTTTATCACACTCAGAGCAACGCTCTTCAGCAGGCGGGATAATTCTTCAACATTTAGAAGATGTGTTATTAAAGGCATACAAAc                                                                         <  1:829341/140‑1 (MQ=255)                                                             aCCCAGTCGGGGATATATTCCATCTTCTCGCTGACATCATCAAGAATAAAAAGGTTTATCACACTCAGAGCAACGCTCTTCAGCAGGCGGGATAATTCTTCAACATTTAGAAGATGTGTTATTAAAGGCATACAAACTgg                                                                      <  1:123970/140‑1 (MQ=255)                                                             aCCCAGTCGGGGATATATTCCATCTTCTCGCTGACATCATCAAGAATAAAAAGGTTTATCACACTCAGAGCAACGCTCTTCAGCAGGCGGGATAATTCTTCAACATTTAGAAGATGTGTTATTAAAGGCATACAAACTgg                                                                      >  1:150901/1‑140 (MQ=255)                                                              cccAGTCGGGGATATATTCCATCTTCTCGCTGACATCATCAAGAATAAAAAGGTTTATCACACTCAGAGCAACGCTCTTCAGCAGGCGGGATAATTCTTCAACATTTAGAAGATGTGTTATTAAAGGCATACAAACTggg                                                                     >  2:697846/1‑139 (MQ=255)                                                              cccAGTCGGGGATATATTCCATCTTCTCGCTGACATCATCAAGAATAAAAAGGTTTATCACACTCAGAGCAACGCTCTTCAGCAGGCGGGATAATTCTTCAACATTTAGAAGATGTGTTATTAAAGGCATACAAACTGGa                                                                     >  2:998577/1‑140 (MQ=255)                                                                 aGTCGGGGATATATTCCATCTTCTCGCTGACATCATCAAGAATAAAAAGGTTTATCACACTCAGAGCAACGCTCTTCAGCAGGCGGGATAATTCTTCAACATTTAGAAGATGTGTTATTAAAGGCAt                                                                               <  2:481170/127‑1 (MQ=255)                                                                 aGTCGGGGATATATTCCATCTTCTCGCTGACATCATCAAGAATAAAAAGGTTTATCACACTCAGAGCAACGCTCTTCAGCAGGCGGGATAATTCTTCAACATTTAGAAGATGTGTTATTAAAGGCAt                                                                               <  2:1052196/127‑1 (MQ=255)                                                                 aGTCGGGGATATATTCCATCTTCTCGCTGACATCATCAAGAATAAAAAGGTTTATCACACTCAGAGCAACGCTCTTCAGCAGGCGGGATAATTCTTCAACATTTAGAAGATGTGTTATTAAAGGCAt                                                                               >  1:1052196/1‑127 (MQ=255)                                                                 aGTCGGGGATATATTCCATCTTCTCGCTGACATCATCAAGAATAAAAAGGTTTATCACACTCAGAGCAACGCTCTTCAGCAGGCGGGATAATTCTTCAACATTTAGAAGATGTGTTATTAAAGGCATACAAACTGGACgt                                                                  >  1:1235576/1‑140 (MQ=255)                                                                 aGTCGGGGATATATTCCATCTTCTCGCTGACATCATCAAGAATAAAAAGGATTATCACACTCAGAGCAACGCTCTTCAGCAGGCGGGATAATTCTTCAACATTTAGAAGATGTGTTATTAAAGGCAt                                                                               >  1:481170/1‑127 (MQ=255)                                                                  gTCGGGGATATATTCCATCTTCTCGCTGACATCATCAAGAATAAAAAGGTTTATCACACTCAGAGCAACGCTCTTCAGCAGGCGGGATAATTCTTCAACATTTAGAAGATGTGTTATTAAAGGCATACAAACTGGACgta                                                                 <  2:644564/140‑1 (MQ=255)                                                                   tCGGGGATATATTCCATCTTCTCGCTGACATCATCAAGAATAAAAAGGTTTATCACACTCAGAGCAACGCTCTTCAGCAGGCGGGATAATTCTTCAACATTTAGAAGATGTGTTATTAAAGGCATACAAACTGGACgtat                                                                <  2:150901/140‑1 (MQ=255)                                                                        gATATATTCCATCTTCTCGCTGACATCATCAAGAATAAAAAGGTTTATCACACTCAGAGCAACGCTCTTCAGCAGGCGGGATAATTCTTCAACATTTAGAAGATGTGTTATTAAAGGCATACAAACTGGACgtattgtat                                                           <  2:541880/140‑1 (MQ=255)                                                                        gATATATTCCATCTTCTCGCTGACATCATCAAGAATAAAAAGGTTTATCACACTCAGAGCAACGCTCTTCAGCAGGCGGGATAATTCTTCAACATTTAGAAGATGTGTTATTAAAGGCATACAAACTGGACgtattgtat                                                           >  1:1189171/1‑140 (MQ=255)                                                                             atTCCATCTTCTCGCTGACATCATCAAGAATAAAAAGGTTTATCACACTCAGAGCAACGCTCTTCAGCAGGCGGGATAATTCTTCAACATTTAGAAGATGTGTTATTAAAGGCATACAAACTGGACgta                                                                 >  1:916051/1‑129 (MQ=255)                                                                             atTCCATCTTCTCGCTGACATCATCAAGAATAAAAAGGTTTATCACACTCAGAGCAACGCTCTTCAGCAGGCGGGATAATTCTTCAACATTTAGAAGATGTGTTATTAAAGGCATACAAACTGGACgta                                                                 <  2:916051/129‑1 (MQ=255)                                                                               tCCATCTTCTCGCTGACATCATCAAGAATAAAAAGGTTTATCACACTCAGAGCAACGCTCTTCAGCAGGCGGGATAATTCTTCAACATTTAGAAGATGTGTTATTAAAGGCATACAAACTGGACGTATTGTATTTTCttt                                                    <  2:1373139/140‑1 (MQ=255)                                                                                 cATCTTCTCGCTGACATCATCAAGAATAAAAAGGTTTATCACACTCAGAGCAACGCTCTTCAGCAGGCGGGATAATTCTTCAACATTTAGAAGATGTGTTATTAAAGGCATACAAACTGGACGTATTGTATTTTCTTTtg                                                  >  1:1363028/1‑140 (MQ=255)                                                                                 cATCTTCTCGCTGACATCATCAAGAATAAAAAGGTTTATCACACTCAGAGCAACGCTCTTCAGCAGGCGGGATAATTCTTCAACATTTAGAAGATGTGTTATTAAAGGCATACAAACTGGACGTATTGTATTTTCTTTtg                                                  >  2:1062248/1‑140 (MQ=255)                                                                                 cATCTTCTCGCTGACATCATCAAGAATAAAAAGGTTTATCACACTCAGAGCAACGCTCTTCAGCAGGCGGGATAATTCTTCAACAATTAGAAGATGTGTTATTAAAGGCATACAAACTGGACGTATTGTATTTTCTTTtg                                                  >  2:937592/1‑140 (MQ=255)                                                                                     ttctCGCTGACATCATCAAGAATAAAAAGGTTTATCACACTCAGAGCAACGCTCTTCAGCAGGCGGGATAATTCTTCAACATTTAGAAGATGTGTTATTAAAGGCATACAAACTGGACGTATTGTATTTTCTTTtggtgg                                              <  2:180113/140‑1 (MQ=255)                                                                                      tctcGCTGACATCATCAAGAATAAAAAGGTTTATCACACTCAGAGCAACGCTCTTCAGCAGGCGGGATAATTCTTCAACATTTAGAAGATGTGTTATTAAAGGCATACAAACTGGACGTATTGTATTTTCTTTTggtggt                                             <  1:1062248/140‑1 (MQ=255)                                                                                           cTGACATCATCAAGAATAAAAAGGTTTATCACACTCAGAGCAACGCTCTTCAGCAGGCGGGATAATTCTTCAACATTTAGAAGATGTGTTATTAAAGGCATACAAACTGGACGTATTGTATTTTCTTTTGGTGGTTGAcc                                        <  2:254587/140‑1 (MQ=255)                                                                                             gACATCATCAAGAATAAAAAGGTTTATCACACTCAGAGCAACGCTCTTCAGCAGGCGGGATAATTCTTCAACATTTAGAAGATGTGTTATTAAAGGCATACAAACTGGACGTATTGTATTTTCTTTTggtggt                                             <  2:349218/133‑1 (MQ=255)                                                                                             gACATCATCAAGAATAAAAAGGTTTATCACACTCAGAGCAACGCTCTTCAGCAGGCGGGATAATTCTTCAACATTTAGAAGATGTGTTATTAAAGGCATACAAACTGGACGTATTGTATTTTCTTTTggtggt                                             >  1:349218/1‑133 (MQ=255)                                                                                             gACATCATCAAGAATAAAAAGGTTTATCACACTCAGAGCAACGCTCTTCAGCAGGCGGGATAATTCTTCAACATTTAGAAGATGTGTTATTAAAGGCATACAAACTGGACGTATTGTATTTTCTTTTGGTGGTTGACCTa                                      <  1:937592/140‑1 (MQ=255)                                                                                                  catcaAGAATAAAAAGGTTTATCACACTCAGAGCAACGCTCTTCAGCAGGCGGGATAATTCTTCAACATTTAGAAGATGTGTTATTGAAGGCATACAAACTGGACGTATTGTATTTTCTTTTGGTGGTTGACCTAAGGTa                                 <  2:581756/140‑1 (MQ=255)                                                                                                  catcaAGAATAAAAAGGTTTATCACACTCAGAGCAACGCTCTTCAGCAGGCGGGATAATTCTTCAACATTTAGAAGATGTGTTATTAAAGGCATACAAACTGGACGTATTGTATTTTCTTTTGGTGGTTGACCTAAGGTa                                 <  1:1073875/140‑1 (MQ=255)                                                                                                    tcaAGAATAAAAAGGTTTATCACACTCAGAGCAACGCTCTTCAGCAGGCGGGATAATTCTTCAACATTTAGAAGATGTGTTATTAAAGGCATACAAACTGGACGTATTGTATTTTCTTTTggtggt                                             >  2:842001/1‑126 (MQ=255)                                                                                                    tcaAGAATAAAAAGGTTTATCACACTCAGAGCAACGCTCTTCAGCAGGCGGGATAATTCTTCAACATTTAGAAGATGTGTTATTAAAGGCATACAAACTGGACGTATTGTATTTTCTTTTggtggt                                             <  1:842001/126‑1 (MQ=255)                                                                                                       aGAATAAAAAGGTTTATCACACTCAGAGCAACGCTCTTCAGCAGGCGGGATAATTCTTCAACATTTAGAAGATGTGTTATTAAAGGCATACAAACTGGACGTATTGTATTTTCTTTTGGTGGTTGACCTAAGGTAGCAGt                            <  2:268714/140‑1 (MQ=255)                                                                                                        gAATAAAAAGGTTTATCACACTCAGAGCAACGCTCTTCAGCAGGCGGGATAATTCTTCAACATTTAGAAGATGTGTTATTAAAGGCATACAAACTGGACGTATTGTATTTTCTTTTGGTGGTTGACCTAAGGTAGCAGtt                           >  2:1031563/1‑140 (MQ=255)                                                                                                        gAATAAAAAGGTTTATCACACTCAGAGCAACACTCTTCAGCAGGCGGGATAATTCTTCAACATTTAGAAGATGTGTTATTAAAGGCATACAAACTGGACGTATTGTATTTTCTTTTGGTGGTTGACCTAAGGTAGCAGtt                           >  1:50089/1‑140 (MQ=255)                                                                                                          aTAAAAAGGTTTATCACACTCAGAGCAACGCTCTTCAGCAGGCGGGATAATTCTTCAACATTTAGAAGATGTGTTATTAAAGGCATACAAACTGGACGTATTGTATTTTCTTTTGGTGGTTGACCTAAGGTAGCAGTTTa                         >  1:419964/1‑140 (MQ=255)                                                                                                          aTAAAAAGGTTTATCACACTCAGAGCAACGCTCTTCAGCAGGCGGGATAATTCTTCAACATTTAGAAGATGTGTTATTAAAGGCATACAAACTGGACGTATTGTATTTTCTTTTGGTGGTTGACCTAAGGTAGCAGTTTa                         >  2:469014/1‑140 (MQ=255)                                                                                                             aaaaGGTTTATCACACTCAGAGCAACGCTCTTCAGCAGGCGGGATAATTCTTCAACATTTAGAAGATGTGTTATTAAAGGCATACAAACTGGACGTATTGTATTTTTTTTTGGTGGTTGACCTAAGGTAGCAGTTTATcc                      >  1:1014751/1‑140 (MQ=255)                                                                                                                            cTCAGAGCAACGCTCTTCAGCAGGCGGGATAATTCTTCAACATTTAGAAGATGTGTTATTAAAGGCATACAAACTGGACGTATTGTATTTTCTTTTGGTGGTTGACCTAAGGTAGCAGTTTATCCTGATGCGCTGAGAtt       >  1:1021694/1‑140 (MQ=255)                                                                                                                            cTCAGAGCAACGCTCTTCAGCAGGCGGGATAATTCTTCAACATTTAGAAGATGTGTTATTAAAGGCATACAAACTGGACGTATT‑TATTTTCTTTTGGTGGTTGACCTAAGGTAGCAGTTTATCCTGATGCGCTGAGAttt      >  1:897822/1‑140 (MQ=255)                                                                                                                             tCAGAGCAACGCTCTTCAGCAGGCGGGATAATTCTTCAACATTTAGAAGATGTGTTATTAAAGGCATACAAACTGGACGTATTGTATTTTCTTTTGGTGGTTGACCTAAGGTAGCAGTTTATCCTGATGCGCTGAGAttt      >  2:717731/1‑140 (MQ=255)                                                                                                                             tCAGAGCAACGCTCTTCAGCAGGCGGGATAATTCTTCAACATTTAGAAGATGTGTTATTAAAGGCATACAAACTGGACGTATTGTATTTTCTTTTGGTGGTTGACCTAAGGTAGCAGTTTATCCTGATGCGCTGAGAttt      <  1:356495/140‑1 (MQ=255)                                                                                                                               agagCAACGCTCTTCAGCAGGCGGGATAATTATTCAACATTTAGAAGATGTGTTATTAAAGGCATACAAACTGGACGTATTGTATTTTCTTTTGGTGGTTGACCTAAGGTAGCAGTTTATCCTGATGCGCTGAGATTTCt    >  2:441391/1‑140 (MQ=255)                                                                                                                                 agCAACGCTCTTCAGCAGGCGGGATAATTCTTCAACATTTAGAAGATGTGTTATTAAAGGCATACAAACTGGACGTATTGTATTTTCTTTTGGTGGTTGACCTAAGGTAGCAGTTTATCCTGATGCGCTGAGATTTCTGt  >  1:8122/1‑140 (MQ=255)                                                                                                                                 agCAACGCTCTTCAGCAGGCGGGATAATTCTTCAACATTTAGAAGATGTGTTATTAAAGGCATACAAACTGGACGTATTGTATTTTCTTTTGGTGGTTGACCTAAGGTAGCAGTTTATCCTGATGCGCTGAGATTTCTGt  >  2:1251535/1‑140 (MQ=255)                                                                                                                                        |                                                                                                                                     CAACAGAATGTGACTTGCCAGATTTATTAGCGCCACCAGATGTTTAACCGGGTAACCACGACCCAGTCGGGGATATATTCCATCTTCTCGCTGACATCATCAAGAATAAAAAGGTTTATCACACTCAGAGCAACGTTCTTCAGCAGGCGGGATAATTCTTCAACATTTAGAAGATGTGTTATTAAAGGCATACAAACTGGACGTATTGTATTTTCTTTTGGTGGTTGACCTAAGGTAGCAGTTTATCCTGATGCGCTGAGATTTCTGT  >  NC_000913/2067402‑2067669

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑