Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,130,566	T→C	T105A (ACA→GCA)	wcaD ←	putative colanic acid polymerase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,130,566	0	T	C	100.0%	339.6 / NA	90	T105A (ACA→GCA)	wcaD	putative colanic acid polymerase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (36/54);  total (36/54)

TTCGAAATCGACTCCATAATACTGCTGCCACCGGTGAGGATAATTTGTGCCATCTCCACCGCCGCCAGCGCCACCACCAGCCCGACCACCAGATAAAAGAAACGTAATATCTTGCGATGGTTGTGTGGCGAAATTGTTTTAAAACGAATACTCCACACCATGCCAATAATGATCACAATATAGACAAACAGCATAGTTGAAGTGACGTATTTACTGGCATCCAGCGACTGACCAAACAGATAGTTAAACGCCGTGAGTCCCGCGCCAATCCCT  >  NC_000913/2130430‑2130702                                                                                                                                         |                                                                                                                                         ttCGAAATCGACTCCATAATACTGCTGCCACCGGTGAGGATAATTTGTGCCATCTCCACCGCCGCCAGCGCCACCACCAGCCCGACCACCAGATAAAAGAAACGTAATATCTTGCGATGGTTGTGTGGCGAAATTGcttt                                                                                                                                       >  2:143493/1‑140 (MQ=255)   cGAAATCGACTCCATAATACTGCTGCCACCGGTGAGGATAATTTGTGCCATCTCCACCGCCGCCAGCGCCACCACCAGCCCGACCACCAGATAAAAGAAACGTAATATCTTGCGATGGTTGTGTGGCGAAATTGCTTTaa                                                                                                                                     <  1:111138/140‑1 (MQ=255)        tCGACTCCATAATACTGCTGCCACCGGTGAGGATAATTTGTGCCATCTCCACCGCCGCCAGCGCCACCACCAGCCCGACCACCAGATAAAAGAAACGTAATATCTTGCGATGGTTGTGTGGCGAAATTGCTTTAAAACGa                                                                                                                                <  1:1121895/140‑1 (MQ=255)        tCGACTCCATAATACTGCTGCCACCGGTGAGGATAATTTGTGCCATCTCCACCGCCGCCAGCGCCACCACCAGCCCGACCACCAGATAAAAGAAACGTAATATCTTGCGATGGTTGTGTGGCGAAATTGCTTTAAAACGa                                                                                                                                <  1:195742/140‑1 (MQ=255)        tCGACTCCATAATACTGCTGCCACCGGTGAGGATAATTTGTGCCATCTCCACCGCCGCCAGCGCCACCACCAGCCCGACCACCAGATAAAAGAAACGTAATATCTTGCGATGGTTGTGTGGCGAAATTGCTTTAAAACGa                                                                                                                                <  1:143493/140‑1 (MQ=255)        tCGACTCCATAATACTGCTGCCACCGGTGAGGATAATTTGTGCCATCTCCACCGCCGCCAGCGCCACCACCAGCCCGACCACCAGATAAAAGAAACGTAATATCTTGCGATGGTTGTGTGGCGAAATTGCTTTAAAACGa                                                                                                                                <  1:415693/140‑1 (MQ=255)        tCGACTCCATAATACTGCTGCCACCGGTGAGGATAATTTGTGCCATCTCCACCGCCGCCAGCGCCACCACCAGCCCGACCACCAGATAAAAGAAACGTAATATCTTGCGATGGTTGTGTGGCGAAATTGCTTTAAAACGa                                                                                                                                <  1:1154209/140‑1 (MQ=255)        tCGACTCCATAATACTGCTGCCACCGGTGAGGATAATTTGTGCCATCTCCACCGCCGCCAGCGCCACCACCAGCCCGACCACCAGATAAAAGAAACGTAATATCTTGCGATGGTTGTGTGGCGAAATTGCTTTAAAACGa                                                                                                                                <  1:497497/140‑1 (MQ=255)        tCGACTCCATAATACTGCTGCCACCGGTGAGGATAATTTGTGCCATCTCCACCGCCGCCAGCGCCACCACCAGCCCGACCACCAGATAAAAGAAACGTAATATCTTGCGATGGTTGTGTGGCGAAATTGCTTTAAAACGa                                                                                                                                <  1:802093/140‑1 (MQ=255)         cGACTCCATAATACTGCTGCCACCGGTGAGGATAATTTGTGCCATCTCCACCGCCGCCAGCGCCACCACCAGCCCGACCACCAGATAAAAGAAACGTAATATCTTGCGATGGTTGTGTGGCGAAATTGCTTTAAAACGaa                                                                                                                               <  1:1092244/140‑1 (MQ=255)         cGACTCCATAATACTGCTGCCACCGGTGAGGATAATTTGTGCCATCTCCACCGCCGCCAGCGCCACCACCAGCCCGACCACCAGATAAAAGAAACGTAATATCTTGCGATGGTTGTGTGGCGAAATTGCTTTAAAACGaa                                                                                                                               <  2:1084175/140‑1 (MQ=255)         cGACTCCATAATACTGCTGCCACCGGTGAGGATAATTTGTGCCATCTCCACCGCCGCCAGCGCCACCACCAGCCCGACCACCAGATAAAAGAAACGTAATATCTTGCGATGGTTGTGTGGCGAAATTGCTTTAAAACGaa                                                                                                                               <  1:88826/140‑1 (MQ=255)         cGACTCCATAATACTGCTGCCACCGGTGAGGATAATTTGTGCCATCTCCACCGCCGCCAGCGCCACCACCAGCCCGACCACCAGATAAAAGAAACGTAATATCTTGCGATGGTTGTGTGGCGAAATTGCTTTAAAACGaa                                                                                                                               <  2:984625/140‑1 (MQ=255)           aCTCCATAATACTGCTGCCACCGGTGAGGATAATTTGTGCCATCTCCACCGCCGCCAGCGCCACCACCAGCCCGACCACCAGATAAAAGAAACGTAATATCTTGCGATGGTTGTGTGGCGAAATTGCTTTAAAACGa                                                                                                                                <  1:110438/137‑1 (MQ=255)           aCTCCATAATACTGCTGCCACCGGTGAGGATAATTTGTGCCATCTCCACCGCCGCCAGCGCCACCACCAGCCCGACCACCAGATAAAAGAAACGTAATATCTTGCGATGGTTGTGTGGCGAAATTGCTTTAAAACGa                                                                                                                                >  2:110438/1‑137 (MQ=255)           aCTCCATAATACTGCTGCCACCGGTGAGGATAATTTGTGCCATCTCCACCGCCGCCAGCGCCACCACCAGCCCGACCACCAGATAAAAGAAACGTAATATCTTGCGATGGTTGTGTGGCGAAATTGCTTTAAAACGAATa                                                                                                                             >  1:919841/1‑140 (MQ=255)                  aataCTGCTGCCACCGGTGAGGATAATTTGTGCCATCTCCACCGCCGCCAGCGCCACCACCAGCCCGACCACCAGATAAAAGAAACGTAATATCTTGCGATGGTTGTGTGGCGAAATTGCTTTAAAACGAATACTCcaca                                                                                                                      <  2:1285980/140‑1 (MQ=255)                         ctgcCACCGGTGAGGATAATTTGTGCCATCTCCACCGCCGCCAGCGCCACCACCAGCCCGACCACCAGATAAAAGAAACGTAATATCTTGCGATGGTTGTGTGGCGAAATTGCTTTAAAACGaa                                                                                                                               >  2:1006728/1‑124 (MQ=255)                         ctgcCACCGGTGAGGATAATTTGTGCCATCTCCACCGCCGCCAGCGCCACCACCAGCCCGACCACCAGATAAAAGAAACGTAATATCTTGCGATGGTTGTGTGGCGAAATTGCTTTAAAACGaa                                                                                                                               >  1:453104/1‑124 (MQ=255)                         ctgcCACCGGTGAGGATAATTTGTGCCATCTCCACCGCCGCCAGCGCCACCACCAGCCCGACCACCAGATAAAAGAAACGTAATATCTTGCGATGGTTGTGTGGCGAAATTGCTTTAAAACGaa                                                                                                                               <  2:453104/124‑1 (MQ=255)                         ctgcCACCGGTGAGGATAATTTGTGCCATCTCCACCGCCGCCAGCGCCACCACCAGCCCGACCACCAGATAAAAGAAACGTAATATCTTGCGATGGTTGTGTGGCGAAATTGCTTTAAAACGaa                                                                                                                               <  1:1006728/124‑1 (MQ=255)                         ctgcCACCGGTGAGGATAATTTGTGCCATCTCCACCGCCGCCAGCGCCACCACCAGCCCGACCACCAGATAAAAGAAACGTAATATCTTGCGATGGTTGTGTGGCGAAATTGCTTTAAAACGa                                                                                                                                >  1:756788/1‑123 (MQ=255)                         ctgcCACCGGTGAGGATAATTTGTGCCATCTCCACCGCCGCCAGCGCCACCACCAGCCCGACCACCAGATAAAAGAAACGTAATATCTTGCGATGGTTGTGTGGCGAAATTGCTTTAAAACGa                                                                                                                                <  2:756788/123‑1 (MQ=255)                         ctgcCACCGGTGAGGATAATTTGTGCCATCTCCACCGCCGCCAGCGCCACCACCAGCCCGACCACCAGATAAAAGAAACGTAATATCTTGCGATGGTTGTGTGGCGAAATTGCTTTAAAACGAATACTCca                                                                                                                        >  1:1017378/1‑131 (MQ=255)                         ctgcCACCGGTGAGGATAATTTGTGCCATCTCCACCGCCGCCAGCGCCACCACCAGCCCGACCACCAGATAAAAGAAACGTAATATCTTGCGATGGTTGTGTGGCGAAATTGCTTTAAAACGAATACTCca                                                                                                                        <  2:1017378/131‑1 (MQ=255)                         ctgcCACCGGTGAGGATAATTTGTGCCATCTCCACCGCCGCCAGCGCCACCACCAGCCCGACCACCAGATAAAAGAAACGTAATATCTTGCGATGGTTGTGTGGCGAAATTGCTTTAAAACGAATACTCCacac                                                                                                                     >  2:448347/1‑134 (MQ=255)                         ctgcCACCGGTGAGGATAATTTGTGCCATCTCCACCGCCGCCAGCGCCACCACCAGCCCGACCACCAGATAAAAGAAACGTAATATCTTGCGATGGTTGTGTGGCGAAATTGCTTTAAAACGAATACTCCacac                                                                                                                     <  1:448347/134‑1 (MQ=255)                         ctgcCACCGGTGAGGATAATTTGTGCCATCTCCACCGCCGCCAGCGCCACCACCAGCCCGACCACCAGATAAAAGAAACGTAATATCTTGCGATGGTTGTGTGGCGAAATTGCTTTAAAACGAATACTCCACACCATGcc                                                                                                               >  2:1351903/1‑140 (MQ=255)                         ctgcCACCGGTGAGGATAATTTGTGCCATCTCCACCGCCGCCAGCGCCACCACCAGCCCGACCACCAGATAAAAGAAACGTAATATCTTGCGATGGTTGTGTGGCGAAATTGCTTTAAAACGAATACTCCACACCATGcc                                                                                                               >  1:1393503/1‑140 (MQ=255)                         ctgcCACCGGTGAGGATAATTTGTGCCATCTCCACCGCCGCCAGCGCCACCACCAGCCCGACCACCAGATAAAAGAAACGTAATATCTTGCGATGGTTGTGTGGCGAAATTGCTTTAAAACGAATACTCCACACCATGcc                                                                                                               >  2:1112946/1‑140 (MQ=255)                         ctgcCACCGGTGAGGATAATTTGTGCCATCTCCACCGCCGCCAGCGCCACCACCAGCCCGACCACCAGATAAAAGAAACGTAATATCTTGCGATGGTTGTGTGGCGAAATTGCTTTAAAACGAATACTCCACACCATGcc                                                                                                               >  2:912979/1‑140 (MQ=255)                         ctgcCACCGGTGAGGATAATTTGTGCCATCTCCACCGCCGCCAGCGCCACCACCAGCCCGACCACCAGATAAAAGAAACGTAATATCTTGCGATGGTTGTGTGGCGAAATTGCTTTAAAACGAATACTCCACACCATGcc                                                                                                               >  2:589219/1‑140 (MQ=255)                         ctgcCACCGGTGAGGATAATTTGTGCCATCTCCACCGCCGCCAGCGCCACCACCAGCCCGACCACCAGATAAAAGAAACGTAATATCTTGCGATGGTTGTGTGGCGAAATTGCTTTAAAACGAATACTCCACACCATGcc                                                                                                               >  2:707946/1‑140 (MQ=255)                          tgcCACCGGTGAGGATAATTTGTGCCATCTCCACCGCCGCCAGCGCCACCACCAGCCCGACCACCAGATAAAAGAAACGTAATATCTTGCGATGGTTGTGTGGCGAAATTGCTTTAAAACGAATACTCCACACCATGCCa                                                                                                              <  1:665993/140‑1 (MQ=255)                           gcCACCGGTGAGGATAATTTGTGCCATCTCCACCGCCGCCAGCGCCACCACCAGCCCGACCACCAGATAAAAGAAACGTAATATCTTGCGATGGTTGTGTGGCGAAATTGCTTTAAAACGaa                                                                                                                               <  1:1262761/122‑1 (MQ=255)                           gcCACCGGTGAGGATAATTTGTGCCATCTCCACCGCCGCCAGCGCCACCACCAGCCCGACCACCAGATAAAAGAAACGTAATATCTTGCGATGGTTGTGTGGCGAAATTGCTTTAAAACGaa                                                                                                                               >  2:1262761/1‑122 (MQ=255)                           gcCACCGGTGAGGATAATTTGTGCCATCTCCACCGCCGCCAGCGCCACCACCAGCCCGACCACCAGATAAAAGAAACGTAATATCTTGCGATGGTTGTGTGGCGAAATTGCTTTAAAACGAATACTCCACACCATGCCaa                                                                                                             >  1:1418560/1‑140 (MQ=255)                            ccACCGGTGAGGATAATTTGTGCCATCTCCACCGCCGCCAGCGCCACCACCAGCCCGACCACCAGATAAAAGAAACGTAATATCTTGCGATGGTTTTGTGGCGAAATTGCTTTAAAACGAATACTCCACACCATGCCaat                                                                                                            >  2:426014/1‑140 (MQ=255)                            ccACCGGTGAGGATAATTTGTGCCATCTCCACCGCCGCCAGCGCCACCACCAGCCCGACCACCAGATAAAAGAAACGTAATATCTTGCGATGGTTGTGTGGCGAAATTGCTTTAAAACGAATACTCCACACCATGCCaat                                                                                                            >  2:508752/1‑140 (MQ=255)                             cACCGGTGAGGATAATTTGTGCCATCTCCACCGCCGCCAGCGCCACCACCAGCCCGACCACCAGATAAAAGAAACGTAATATCTTGCGATGGTTGTGTGGCGAAATTGCTTTAAAACGAATACTCCACACCATGCCaata                                                                                                           <  1:1015303/140‑1 (MQ=255)                              aCCGGTGAGGATAATTTGTGCCATCTCCACCGCCGCCAGCGCCACCACCAGCCCGACCACCAGATAAAAGAAACGTAATATCTTGCGATGGTTGTGTGGCGAAATTGCTTTAAAACGAATACTCCACACCATGCCaataa                                                                                                          >  2:774284/1‑140 (MQ=255)                              aCCGGTGAGGATAATTTGTGCCATCTCCACCGCCGCCAGCGCCACCACCAGCCCGACCACCAGATAAAAGAAACGTAATATCTTGCGATGGTTGTGTGGCGAAATTGCTTTAAAACGAATACTCCACACCATGCCaataa                                                                                                          >  1:939786/1‑140 (MQ=255)                               ccGGTGAGGATAATTTGTGCCATCTCCACCGCCGCCAGCGCCACCACCAGCCCGACCACCAGATAAAAGAAACGTAATATCTTGCGATGGTTGTGTGGCGAAATTGCTTTAAAACGAATACTCCACACCATGCCaataat                                                                                                         <  2:512818/140‑1 (MQ=255)                                  gTGAGGATAATTTGTGCCATCTCCACCGCCGCCAGCGCCACCACCAGCCCGACCACCAGATAAAAGAAACGTAATATCTTGCGATGGTTGTGTGGCGAAATTGCTTTAAAACGAATACTCCACACCATGCCAATAATGAt                                                                                                      <  1:912979/140‑1 (MQ=255)                                  gTGAGGATAATTTGTGCCATCTCCACCGCCGCCAGCGCCACCACCAGCCCGACCACCAGATAAAAGAAACGTAATATCTTGCGATGGTTGTGTGGCGAAATTGCTTTAAAACGAATACTCCACACCATGCCAATAATGAt                                                                                                      <  1:1079551/140‑1 (MQ=255)                                   tGAGGATAATTTGTGCCATCTCCACCGCCGCCAGCGCCACCACCAGCCCGACCACCAGATAAAAGAAACGTAATATCTTGCGATGGTTGTGTGGCGAAATTGCTTTAAAGCGAATACTCCACACCATGCCAATAATGATc                                                                                                     <  2:1364324/140‑1 (MQ=255)                                   tGAGGATAATTTGTGCCATCTCCACCGCCGCCAGCGCCACCACCAGCCCGACCACCAGATAAAAGAAACGTAATATCTTGCGATGGTTGTGTGGCGAAATTGCTTTAAAACGAATACTCCACACCATGCCAATAATGATc                                                                                                     <  2:919841/140‑1 (MQ=255)                                   tGAGGATAATTTGTGCCATCTCCACCGCCGCCAGCGCCACCACCAGCCCGACCACCAGATAAAAGAAACGTAATATCTTGCGATGGTTGTGTGGCGAAATTGCTTTAAAACGAATACTCCACACCATGCCAATAATGATc                                                                                                     <  2:1137049/140‑1 (MQ=255)                                   tGAGGATAATTTGTGCCATCTCCACCGCCGCCAGCGCCACCACCAGCCCGACCACCAGATAAAAGAAACGTAATATCTTGCGATGGTTGTGTGGCGAAATTGCTTTAAAACGAATACTCCACACCATGCCAATAATGATc                                                                                                     <  1:1112946/140‑1 (MQ=255)                                   tGAGGATAATTTGTGCCATCTCCACCGCCGCCAGCGCCACCACCAGCCCGACCACCAGATAAAAGAAACGTAATATCTTGCGATGGTTGTGTGGCGAAATTGCTTTAAAACGAATACTCCACACCATGCCAATAATGATc                                                                                                     <  1:128681/140‑1 (MQ=255)                                   tGAGGATAATTTGTGCCATCTCCACCGCCGCCAGCGCCACCACCAGCCCGACCACCAGATAAAAGAAACGTAATATCTTGCGATGGTTGTGTGGCGAAATTGCTTTAAAACGAATACTCCACACCATGCCAATAATGATc                                                                                                     >  2:963254/1‑140 (MQ=255)                                    gAGGATAATTTGTGCCATCTCCACCGCCGCCAGCGCCACCACCAGCCCGACCACCAGATAAAAGAAACGTAATATCTTGCGATGGTTGTGTGGCGAAATTGCTTTAAAACGAATACTCCACACCATGCCAATAATGATca                                                                                                    <  1:964128/140‑1 (MQ=255)                                    gAGGATAATTTGTGCCATCTCCACCGCCGCCAGCGCCACCACCAGCCCGACCACCAGATAAAAGAAACGTAATATCTTGCGATGGTTGTGTGGCGAAATTGCTTTAAAACGAATACTCCACACCATGCCAATAATGATca                                                                                                    <  1:1322867/140‑1 (MQ=255)                                        aTAATTTGTGCCATCTCCACCGCCGCCAGCGCCACCACCAGCCCGACCACCAGATAAAAGAAACGTAATATCTTGCGATGGTTGTGTGGCGAAATTGCTTTAAAACGAATACTCCACACCATGCCAATAATGATCACAat                                                                                                >  2:1106288/1‑140 (MQ=255)                                            tttGTGCCATCTCCACCGCCGCCAGCGCCACCACCAGCCCGACCACCAGATAAAAGAAACGTAATATCTTGCGATGGTTGTGTGGCGAAATTGCTTTAAAACGAATACTCCACACCATGCCAATAATGATCACAATATAg                                                                                            >  2:941197/1‑140 (MQ=255)                                               gtgCCATTTCTGCCGCCGCCCGCGCCACCACCAGCCCGACCACCAGATAAAAGAAACGTAATATCTTGCGATGGTTGTGTGGCGAAATTGCTTTAAAACGAATACTCCACACCATGCCAATAATGATCACAATATAGACa                                                                                         <  2:939786/140‑1 (MQ=255)                                               gtgCCATCTCCACCGCCGCCAGCGCCACCACCAGCCCGACCACCAGATAAAAGAAACGTAATATCTTGCGATGGTTGTGTGGCGAAATTGCTTTAAAACGAATACTCCACACCATGCCAATAATGATCACAATATAGACa                                                                                         <  2:1393503/140‑1 (MQ=255)                                                 gCCATCTCCACCGCCGCCAGCGCCACCACCAGCCCGACCACCAGATAAAAGAAACGTAATATCTTGCGATGGTTGTGTGGCGAAATTGCTTTAAAACGAATACTCCACACCATGCCAATAATGATCACAATATAGACaaa                                                                                       >  1:362386/1‑140 (MQ=255)                                                    aTCTCCACCGCCGCCAGCGCCACCACCAGCCCGACCACCAGATAAAAGAAACGTAATATCTTGCGATGGTTGTGTGGCGAAATTGCTTTAAAACGAATACTCCACACCATGCCAATAATGATCACAATATAGACAAACAg                                                                                    <  2:1418560/140‑1 (MQ=255)                                                     tctcCACCGCCGCCAGCGCCACCACCAGCCCGACCACCAGATAAAAGAAACGTAATATCTTGCGATGGTTGTGTGGCGAAATTGCTTTAAAACGAATACTCCACACCATGCCAATAATGATca                                                                                                    <  2:298288/123‑1 (MQ=255)                                                     tctcCACCGCCGCCAGCGCCACCACCAGCCCGACCACCAGATAAAAGAAACGTAATATCTTGCGATGGTTGTGTGGCGAAATTGCTTTAAAACGAATACTCCACACCATGCCAATAATGATca                                                                                                    >  1:298288/1‑123 (MQ=255)                                                          aCCGCCGCCAGCGCCACCACCAGCCCGACCACCAGATAAAAGAAACGTAATATCCTGCGATGGTTGTGTGGCGAAATTGCTTTAAAACGAATACTCCACACCATGCCAATAATGATCACAATATAGACAAACAGCATAGt                                                                              <  2:1355836/140‑1 (MQ=255)                                                             gccgccAGCGCCACCACCAGCCCGACCACCAGATAAAAGAAACGTAATATCTTGCGATGGTTGTGTGGCGAAATTGCTTTAAAACGAATACTCCACACCATGCCAATAATGATCACAATATAGACAAACAGCATAGTTGa                                                                           >  1:495824/1‑140 (MQ=255)                                                                gccAGCGCCACCACCAGCCCGACCACCAGATAAAAGAAACGTAATATCTTGCGATGGTTGTGTGGCGAAATTGCTTTAAAACGAATACTCCACACCATGCCAATAATGATCACAATATAGACAAACAGCATAGTTGAAGt                                                                        <  2:340745/140‑1 (MQ=255)                                                                gccAGCGCCACCACCAGCCCGACCACCAGATAAAAGAAACGTAATATCTTGCGATGGTTGTGTGGCGAAATTGCTTTAAAACGAATACTCCACACCATGCCAATAATGATCACAATATAGACAAACAGCATAGTTGAAGt                                                                        <  1:1106288/140‑1 (MQ=255)                                                                gccAGCGCCACCACCAGCCCGACCACCAGATAAAAGAAACGTAATATCTTGCGATGGTTGTGTGGCGAAATTGCTTTAAAACGAATACTCCACACCATGCCAATAATGATCACAATATAGACAAACAGCATAGTTGAAGt                                                                        <  1:589219/140‑1 (MQ=255)                                                                   aGCGCCACCACCAGCCCGACCACCAGATAAAAGAAACGTAATATCTTGCGATGGTTGTGTGGCGAAATTGCTTTAAAACGAATACTCCACACCATGCCAATAATGATCACAATATAGACAAACAGCATAGTTGAAGTGAc                                                                     >  1:60333/1‑140 (MQ=255)                                                                      gcCACCACCAGCCCGACCACCAGATAAAAGAAACGTAATATCTTGCGATGGTTGTGTGGCGAAATTGCTTTAAAACGAATACTCCACACCATGCCAATAATGATCACAATATAGACAAACAGCATAGTTGAAGTGACGTa                                                                  <  1:707946/140‑1 (MQ=255)                                                                              caGCCCGACCACCAGATAAAAGAAACGTAATATCTTGCGATGGTTGTGTGGCGAAATTGCTTTAAAACGAATACTCCACACCATGCCAATAATGATCACAATATAGACAAACAGCATAGTTGAACTGACGTATTTACTgg                                                          <  1:426014/140‑1 (MQ=255)                                                                               aGCCCGACCACCAGATAAAAGAAACGTAATATCTTGCGATGGTTGTGTGGCGAAATTGCTTTAAAACGAATACTCCACACCATGCCAATAATGATCACAATATAGACAAACAGCATAGTTGAAGTGACGTATTTACTGGc                                                         >  2:698666/1‑140 (MQ=255)                                                                                     accaccAGATAAAAGAAACGTAATATCTTGCGATGGTTGTGTGGCGAAATTGCTTTAAAACGAATACTCCACACCATGCCAATAATGATCACAATATAGACAAACAGCATAGTTGAAGCGACGCATTTACTGGCATCCAg                                                   >  2:315478/1‑140 (MQ=255)                                                                                              tAAAAGAAACGTAATATCTTGCGATGGTTGTGTGGCGAAATTGCTTTAAAACGAATACTCCACACCATGCCAATAATGATCACAATATAGACAAACAGCATAGTTGAAGTGACGTATTTACTGGCATCCAGCGACTGAcc                                          <  2:1109394/140‑1 (MQ=255)                                                                                                 aaGAAACGTAATATCTTGCGATGGTTGTGTGGCGAAATTGCTTTAAAACGAATACTCCACACCATGCCAATAATGATCACAATATAGACAAACAGCATAGTTGAAGTGACGTATTTACTGGCATCCAGCGACTGACCaaa                                       >  1:226217/1‑140 (MQ=255)                                                                                                 aaGAAACGTAATATCTTGCGATGGTTGTGTGGCGAAATTGCTTTAAAACGAATACTCCACACCATGCCAATAATGATCACAATATAGACAAACAGCATAGTTGAAGTGACGTATTTACTGGCATCCAGCGACTGACCaaa                                       <  1:941197/140‑1 (MQ=255)                                                                                                   gAAACGTAATATCTTGCGATGGTTGTGTGGCGAAATTGCTTTAAAACGAATACTCCACACCATGCCAATAATGATCACAATATAGACAAACAGCATAGTTGAAGTGACGTATTTACTGGCATCCAGCGACTGACCAAACa                                     <  1:774284/140‑1 (MQ=255)                                                                                                      aCGTAATATCTTGCGATGGTTGTGTGGCGAAATTGCTTTAAAACGAATACTCCACACCATGCCAATAATGATCACAATATAGACAAACAGCATAGTTGAAGTGACGTATTTACTGGCATCCAGCGACTGACCAAACAGAt                                  >  2:129072/1‑140 (MQ=255)                                                                                                           atatCTTGCGATGGTTGTGTGGCGAAATTGCTTTAAAACGAATACTCCACACCATGCCAATAATGATCACAATATAGACAAACAGCATAGTTGAAGTGACGTATTTACTGGCATCCAGCGACTGACCAAACAGATAGTTa                             >  1:611862/1‑140 (MQ=255)                                                                                                                   cGATGGTTGTGTGGCGAAATTGCTTTAAAACGAATACTCCACACCATGCCAATAATGATCACAATATAGACAAACAGCATAGTTGAAGTGACGTATTTACTGGCATCCAGCGACTGACCAAACAGATAGTTAAACGCCGt                     <  1:57451/140‑1 (MQ=255)                                                                                                                        gTTGTGTGGCGAAATTGCTTTAAAACGAATACTCCACACCATGCCAATAATGATCACAATATAGACAAACAGCATAGTTGAAGTGACGTATTTACTGGCATCCAGCGACTGACCAAACAGATAGTTAAACGCCGTGAGTc                >  1:427209/1‑140 (MQ=255)                                                                                                                         ttGTGTGGCGAAATTGCTTTAAAACGAATACTCCACACCATGCCAATAATGATCACAATATAGACAAACAGCATAGTTGAAGTGACGTATTTACTGGCATCCAGCGACTGACCAAACAGATAGTTAAACGCCGTGAGTcc               >  1:1027716/1‑140 (MQ=255)                                                                                                                             gtgGCGAAATTGCTTTAAAACGAATACTCCACACCATGCCAATAATGATCACAATATAGACAAACAGCATAGTTGAAGTGACGTATTTACTGGCATCCAGCGACTGACCAAACAGATAGTTAAACGCCGTGAGTCCcgcg           >  2:881957/1‑140 (MQ=255)                                                                                                                              tgGCGAAATTGCTTTAAAACGAATACTCCACACCATGCCAATAATGATCACAATATAGACAAACAGCATAGTTGAAGTGACGTATTTACTGGCATCCAGCGACTGACCAAACAGATAGTTAAACGCCGTGAGTCCCgcgc          >  1:985288/1‑140 (MQ=255)                                                                                                                                gCGAAATTGCTTTAAAACGAATACTCCACACCATGCCAATAATGATCACAATATAGACAAACAGCATAGTTGAAGTGACGTATTTACTGGCATCCAGCGACTGACCAAACAGATAGTTAAACGCCGTGAGTCCCGCGCCa        <  2:985288/140‑1 (MQ=255)                                                                                                                                gCGAAATTGCTTTAAAACGAATACTCCACACCATGCCAATAATGATCACAATATAGACAAACAGCATAGTTGAAGTGACGTATTTACTGGCATCCAGCGACTGACCAAACAGATAGTTAAACGCCGTGAGTCCCGCGCCa        <  1:129072/140‑1 (MQ=255)                                                                                                                                gCGAAATTGCTTTAAAACGAATACTCCACACCATGCCAATAATGATCACAATATAGACAAACAGCATAGTTGAAGTGACGTATTTACTGGCATCCAGCGACTGACCAAACAGATAGTTAAACGCCGTGAGTCCCGCGCCa        <  1:698666/140‑1 (MQ=255)                                                                                                                                gCGAAATTGCTTTAAAACGAATACTCCACACCATGCCAATAATGATCACAATATAGACAAACAGCATAGTTGAAGTGACGTATTTACTGGCATCCAGCGACTGACCAAACAGATAGTTAAACGCCGTGAGTCCCGCGCCa        <  1:881957/140‑1 (MQ=255)                                                                                                                                gCGAAATTGCTTTAAAACGAATACTCCACACCATGCCAATAATGATCACAATATAGACAAACAGCATAGTTGAAGTGACGTATTTACTGGCATCCAGCGACTGACCAAACAGATAGTTAAACGCCGTGAGTCCCGCGCCa        <  1:315478/140‑1 (MQ=255)                                                                                                                                gCGAAATTGCTTTAAAACGAATACTCCACACCATGCCAATAATGATCACAATATAGACAAACAGCATAGTTGAAGTGACGTATTTACTGGCATCCAGCGACTGACCAAACAGATAGTTAAACGCCGTGAGTCCCGCGCCa        >  1:1113796/1‑140 (MQ=255)                                                                                                                                gCGAAATTGCTTTAAAACGAATACTCCACACCATGCCAATAATGATCACAATATAGACAAACAGCATAGTTGAAGTGACGTATTTACTGGCATCCAGCGACTGACCAAACAGATAGTTAAACGCCGTGAGTCCCGCGCCa        <  2:495824/140‑1 (MQ=255)                                                                                                                                   aaaTTGCTTTAAAACGAATACTCCACACCATGCCAATAATGATCACAATATAGACAAACAGCATAGTTGAAGTGACGTATTTACTGGCATCCAGCGACTGACCAAACAGATAGTTAAACGCCGTGAGTCCCGCGCCAATc     <  2:362386/140‑1 (MQ=255)                                                                                                                                      ttGCTTTAAAACGAATACTCCACACCATGCCAATAATGATCACAATATAGACAAACAGCATAGTTGAAGTGACGTATTTACTGGCATCCAGCGACTGACCAAACAGATAGTTAAACGCCGTGAGTCCCGCGCCAATCCCt  <  1:963254/140‑1 (MQ=255)                                                                                                                                      ttGCTTTAAAACGAATACTCCACACCATGCCAATAATGATCACAATATAGACAAACAGCATAGTTGAAGTGACGTATTTACTGGCATCCAGCGACTGACCAAACAGATAGTTAAACGCCGTGAGTCCCGCGCCAATCCCt  >  1:154530/1‑140 (MQ=255)                                                                                                                                         |                                                                                                                                         TTCGAAATCGACTCCATAATACTGCTGCCACCGGTGAGGATAATTTGTGCCATCTCCACCGCCGCCAGCGCCACCACCAGCCCGACCACCAGATAAAAGAAACGTAATATCTTGCGATGGTTGTGTGGCGAAATTGTTTTAAAACGAATACTCCACACCATGCCAATAATGATCACAATATAGACAAACAGCATAGTTGAAGTGACGTATTTACTGGCATCCAGCGACTGACCAAACAGATAGTTAAACGCCGTGAGTCCCGCGCCAATCCCT  >  NC_000913/2130430‑2130702

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑