Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	350,425	A→G	K138K (AAA→AAG)	prpC →	2‑methylcitrate synthase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	350,425	0	A	G	100.0%	214.8 / NA	59	K138K (AAA→AAG)	prpC	2‑methylcitrate synthase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (25/34);  total (25/34)

GTACCGTGCTGGAAGCCTTACCGGCGGCGTCGCACCCGATGGATGTTATGCGCACCGGTGTTTCCGCGCTCGGCTGCACGCTGCCAGAAAAAGAGGGGCATACCGTCTCTGGCGCGCGGGATATTGCCGACAAACTGCTGGCGTCGCTTAGCTCGATTCTCCTTTATTGGTATCACTACAGCCACAACGGCGAACGCATCCAACCGGAAACCGATGACGACTCCATCGGCGGTCACTTCCTGCATCTGCTGCACGGCGAAAAGCCATCG  >  NC_000913/350292‑350560                                                                                                                                      |                                                                                                                                        gTACCGTGCTGGAAGCCTTACCGGCGGCGTCGCACCCGATGGATGTTATGCGCACCGGTGTTTCCGCGCTCGGGTGCACGCTGCCAGAAAAAGAGGGGCATACCGTCTCTGGCGCGCGGGATATTGCCGACAAGctgctg                                                                                                                                   >  2:1228159/1‑140 (MQ=255)  tACCGTGCTGGAAGCCTTACCGGCGGCGTCGCACCCGATGGATGTTATGCGCACCGGTGTTTCCGCGCTCGGCTGCACGCTGCCAGAAAAAGAGGGGCATACCGTCTCTGGCGCGCGGGATATTGCCGACAAGCTGCTgg                                                                                                                                  >  1:770807/1‑140 (MQ=255)   aCCGTGCTGGAAGCCTTACCGGCGGCGTCGCACCCGATGGATGTTATGCGCACCGGTGTTTCCGCGCTCGGCTGCACGCTGCCAGAAAAAGAGGGGCATACCGTCTCTGGCGCGCGGGATATTGCCGACAAGCTGCTGGc                                                                                                                                 <  1:1012602/140‑1 (MQ=255)     cGTGCTGGAAGCCTTACCGGCGGCGTCGCACCCGATGGATGTTATGCGCACCGGTGTTTCCGCGCTCGGCTGCACGCTGCCAGAAAAAGAGGGGCATACCGTCTCTGGCGCGCGGGATATTGCCGACAAGCTGCTGGCGt                                                                                                                               >  2:1224032/1‑140 (MQ=255)       tGCTGGAAGCCTTACCGGCGGCGTCGCACCCGATGGATGTTATGCGCACCGGTGTTTCCGCGCTCGGCTGCACGCTGCCAGAAAAAGAGGGGCATACCGTCTCTGGCGCGCGGGATATTGCCGACAAGCTGCTGGCGTCg                                                                                                                             >  2:1106924/1‑140 (MQ=255)       tGCTGGAAGCCTTACCGGCGGCGTCGCACCCGATGGATGTTATGCGCACCGGTGTTTCCGCGCTCGGCTGCACGCTGCCAGAAAAAGAGGGGCATACCGTCTCTGGCGCGCGGGATATTGCCGACAAGCTGCTGGCGTCg                                                                                                                             >  1:311677/1‑140 (MQ=255)       tGCTGGAAGCCTTACCGGCGGCGTCGCACCCGATGGATGTTATGCGCACCGGTGTTTCCGCGCTCGGCTGCACGCTGCCAGAAAAAGAGGGGCATACCGTCTCTGGCGCGCGGGATATTGCCGACAAGCTGCTGGCGTCg                                                                                                                             >  2:207205/1‑140 (MQ=255)            gAAGCCTTACCGGCGGCGTCGCACCCGATGGATGTTATGCGCACCGGTGTTTCCGCGCTCGGCTGCACGCTGCCAGAAAAAGAGGGGCATACCGTCTCTGGCGCGCGGGATATTGCCGACAAGCTGCTGGCGTCGCTTAg                                                                                                                        <  1:1224032/140‑1 (MQ=255)            gAAGCCTTACCGGCGGCGTCGCACCCGATGGATGTTATGCGCACCGGTGTTTCCGCGCTCGGCTGCACGCTGCCAGAAAAAGAGGGGCATACCGTCTCTGGCGCGCGGGATATTGCCGACAAGCTGCTGGCGTCGCTTAg                                                                                                                        <  1:22382/140‑1 (MQ=255)              aGCCTTACCGGCGGCGTCGCACCCGATGGATGTTATGCGCACCGGTGTTTCCGCGCTCGGCTGCACGCTGCCAGAAAAAGAGGGGCATACCGTCTCTGGCGCGCGGGATATTGCCGACAAGCTGCTGGCGTCGCTTAGCt                                                                                                                      <  2:554416/140‑1 (MQ=255)                 cTTACCGGCGGCGTCGCACCCGATGGATGTTATGCGCACCGGTGTTTCCGCGCTCGGCTGCACGCTGCCAGAAAAAGAGGGGCATACCGTCTCTGGCGCGCGGGATATTGCCGACAAGCTGCTGGCGTCGCt                                                                                                                           >  1:968040/1‑132 (MQ=255)                 cTTACCGGCGGCGTCGCACCCGATGGATGTTATGCGCACCGGTGTTTCCGCGCTCGGCTGCACGCTGCCAGAAAAAGAGGGGCATACCGTCTCTGGCGCGCGGGATATTGCCGACAAGCTGCTGGCGTCGCt                                                                                                                           <  2:968040/132‑1 (MQ=255)                 cTTACCGGCGGCGTCGCACCCGATGGATGTTATGCGCACCGGTGTTTCCGCGCTCGGCTGCACGCTGCCAGAAAAAGAGGGGCATACCGTCTCTGGCGCGCGGGATATTGCCGACAAGCTGCTGGCGTCGCTTAGCTCGa                                                                                                                   >  1:1126556/1‑140 (MQ=255)                   tACCGGCGGCGTCGCACCCGATGGATGTTATGCGCACCGGTGTTTCCGCGCTCGGCTGCACGCTGCCAGAAAAAGAGGGGCATACCGTCTCTGGCGCGCGGGATATTGCCGACAAGCTGCTGGCGTCGCTTAGCTCGAtt                                                                                                                 <  1:1372463/140‑1 (MQ=255)                   tACCGGCGGCGTCGCACCCGATGGATGTTATGCGCACCGGTGTTTCCGCGCTCGGCTGCACGCTGCCAGAAAAAGAGGGGCATACCGTCTCTGGCGCGCGGGATATTGCCGACAAGCTGCTGGCGTCGCTTAGCTCGAtt                                                                                                                 <  1:98172/140‑1 (MQ=255)                   tACCGGCGGCGTCGCACCCGATGGATGTTATGCGCACCGGTGTTTCCGCGCTCGGCTGCACGCTGCCAGAAAAAGAGGGGCATACCGTCTCTGGCGCGCGGGATATTGCCGACAAGCTGCTGGCGTCGCTTAGCTCGAtt                                                                                                                 <  1:1200074/140‑1 (MQ=255)                    aCCGGCGGCGTCGCACCCGATGGATGTTATGCGCACCGGTGTTTCCGCGCTCGGCTGCACGCTGCCAGAAAAAGAGGGGCATACCGTCTCTGGCGCGCGGGATATTGCCGACAAGCTGCTGGCGTCGCTTAGCTCGATtc                                                                                                                >  2:1235742/1‑140 (MQ=255)                     ccGGCGGCGTCGCACCCGATGGATGTTATGCGCACCGGTGTTTCCGCGCTCGGCTGCACGCTGCCAGAAAAAGAGGGGCATACCGTCTCTGGCGCGCGGGATATTGCCGACAAGCTGCTGGCGTCGCTTAGCTCGATtct                                                                                                               <  2:49320/140‑1 (MQ=255)                      cggcggCGTCGCACCCGATGGATGTTATGCGCACCGGTGTTTCCGCGCTCGGCTGCACGCTGCCAGAAAAAGAGGGGCATACCGTCTCTGGCGCGCGGGATATTGCCGACAAGCTGCTGGCGTCGCTTAGCt                                                                                                                      >  1:1394152/1‑132 (MQ=255)                      cggcggCGTCGCACCCGATGGATGTTATGCGCACCGGTGTTTCCGCGCTCGGCTGCACGCTGCCAGAAAAAGAGGGGCATACCGTCTCTGGCGCGCGGGATATTGCCGACAAGCTGCTGGCGTCGCTTAGCt                                                                                                                      <  2:1394152/132‑1 (MQ=255)                       ggcggcGTCGCACCCGATGGATGTTATGCGCACCGGTGTTTCCGCGCTCGGCTGCACGCTGCCAGAAAAAGAGGGGCATACCGTCTCTGGCGCGCGGGATATTGCCGACAAGCTGCTGGCGTCGCTTAGCTCGATTCTcc                                                                                                             <  2:247052/140‑1 (MQ=255)                        gcggcgTCGCACCCGATGGATGTTATGCGCACCGGTGTTTCCGCGCTCGGCTGCACGCTGCCAGAAAAAGAGGGGCATACCGTCTCTGGCGCGCGGGATATTGCCGACAAGCTGCTGGCGTCGCTTAGCTCGATTCTCCt                                                                                                            <  1:881412/140‑1 (MQ=255)                           gcgTCGCACCCGATGGATGTTATGCGCACCGGTGTTTCCGCGCTCGGCTGCACGCTGCCAGAAAAAGAGGGGCATACCGTCTCTGGCGCGCGGGATATTGCCGACAAGCTGCTGGCGTCGCTTAGCTCGATTCTCCTTTa                                                                                                         <  2:311677/140‑1 (MQ=255)                            cgTCGCACCCGATGGATGTTATGCGCACCGGTGTTTCCGCGCTCGGGTGCACGCTGCCAGAAAAAGAGGGGCATACCGTCTCTGGCGCGCGGGATATTGCCGACAAGCTGCTGGCGTCGCTTAGCTCGATTCTCCTTTAt                                                                                                        <  1:1228159/140‑1 (MQ=255)                            cgTCGCACCCGATGGATGTTATGCGCACCGGTGTTTCCGCGCTCGGCTGCACGCTGCCAGAAAAAGAGGGGCATACCGTCTCTGGCGCGCGGGATATTGCCGACAAGCTGCTGGCGTCGCTTAGCTCGATTCTCCTTTAt                                                                                                        >  2:469035/1‑140 (MQ=255)                            cgTCGCACCCGATGGATGTTATGCGCACCGGTGTTTCCGCGCTCGGCTGCACGCTGCCAGAAAAAGAGGGGCATACCGTCTCTGGCGCGCGGGATATTGCCGACAAGCTGCTGGCGTCGCTTAGCTCGATTCTCCTTTAt                                                                                                        <  1:734402/140‑1 (MQ=255)                            cgTCGCACCCGATGGATGTTATGCGCACCGGTGTTTCCGCGCTCGGCTGCACGCTGCCAGAAAAAGAGCGGCATACCGTCTCTGGCGCGCGGGATATTGCCGACAAGCTGCTGGCGTCGCTTAGCTCGATTCTCCTTTAt                                                                                                        <  2:987773/140‑1 (MQ=255)                             gTCGCACCCGATGGATGTTATGCGCACCGGTGTTTCCGCGCTCGGCTGCACGCTGCCAGAAAAAGAGGGGCATACCGTCTCTGGCGCGCGGGATATTGCCGACAAGCTGCTGGCGTCGCTTAGCTCGATTCTCCTTTAtt                                                                                                       <  1:620396/140‑1 (MQ=255)                             gTCGCACCCGATGGATGTTATGCGCACCGGTGTTTCCGCGCTCGGCTGCACGCTGCCAGAAAAAGAGGGGCATACCGTCTCTGGCGCGCGGGATATTGCCGACAAGCTGCTGGCGTCGCTTAGCTCGATTCTCCTTTAtt                                                                                                       <  1:269555/140‑1 (MQ=255)                              tCGCACCCGATGGATGTTATGCGCACCGGTGTTTCCGCGCTCGGCTGCACGCTGCCAGAAAAAGAGGGGCATACCGTCTCTGGCGCGCGGGATATTGCCGACAAGCTGCTGGCGTCGCTTAGCTCGATTCTCCTTTATTg                                                                                                      >  1:754779/1‑140 (MQ=255)                                gCACCCGATGGATGTTATGCGCACCGGTGTTTCCGCGCTCGGCTGCACGCTGCCAGAAAAAGAGGGGCATACCGTCTCTGGCGCGCGGGATATTGCCGACAAGCTGCTGGCGTCGCTTAGCTCGATTCTCCTTTATTGGt                                                                                                    <  2:1174071/140‑1 (MQ=255)                                   cccGATGGATGTTATGCGCACCGGTGTTTCCGCGCTCGGCTGCACGCTGCCAGAAAAAGAGGGGCATACCGTCTCTGGCGCGCGGGATATTGCCGACAAGCTGCTGGCGTCGCTTAGCTCGATTCTCCTTTa                                                                                                         >  2:260496/1‑132 (MQ=255)                                   cccGATGGATGTTATGCGCACCGGTGTTTCCGCGCTCGGCTGCACGCTGCCAGAAAAAGAGGGGCATACCGTCTCTGGCGCGCGGGATATTGCCGACAAGCTGCTGGCGTCGCTTAGCTCGATTCTCCTTTa                                                                                                         <  1:260496/132‑1 (MQ=255)                                    ccGATGGATGTTATGCGCACCGGTGTTTCCGCGCTCGGCTGCACGCTGCCAGAAAAAGAGGGGCATACCGTCTCTGGCGCGCGGGATATTGCCGACAAGCTGCTGGCGTCGCTTAGCTCGATTCTCCTTTATTGGTATCa                                                                                                <  2:1315671/140‑1 (MQ=255)                                             gTTATGCGCACCGGTGTTTCCGCGCTCGGCTGCACGCTGCCAGAAAAAGAGGGGCATACCGTCTCTGGCGCGCGGGATATTGCCGACAAGCTGCTGGCGTCGCTTAGCTCGATTCTCCTTTATTGGTATCACTACAGCca                                                                                       <  1:1106924/140‑1 (MQ=255)                                                   cgcACCGGTGTTTCCGCGCTCGGCTGCACGCTGCCAGAAAAAGAGGGGCATACCGTCTCTGGCGCGCGGGATATTGCCGACAAGCTGCTGGCGTCGCTTAGCTCGATTCTCCTTTATTGGTATCACTACAGCCACAACgg                                                                                 <  1:469035/140‑1 (MQ=255)                                                        cGGTGTTTCCGCGCTCGGCTGCACGCTGCCAGAAAAAGAGGGGCATACCGTCTCTGGCGCGCGGGATATTGCCGACAAGCTGCTGGCGTCGCTTAGCTCGATTCTCCTTTATTGGTATCACTACAGCCACAACgg                                                                                 >  2:342344/1‑135 (MQ=255)                                                        cGGTGTTTCCGCGCTCGGCTGCACGCTGCCAGAAAAAGAGGGGCATACCGTCTCTGGCGCGCGGGATATTGCCGACAAGCTGCTGGCGTCGCTTAGCTCGATTCTCCTTTATTGGTATCACTACAGCCACAACgg                                                                                 <  1:342344/135‑1 (MQ=255)                                                             tttCCGCGCTCGGCTGCACGCTGCCAGAAAAAGAGGGGCATACCGTCTCTGGCGCGCGGGATATTGCCGACAAGCTGCTGGCGTCGCTTAGCTCGATTCTCCTTTATTGGTATCACTACAGCCACAACGGCGAACGCATc                                                                       <  1:1235742/140‑1 (MQ=255)                                                             tttCCGCGCTCGGCTGCACGCTGCCAGAAAAAGAGGGGCATACCGTCTCTGGCGCGCGGGATATTGCCGACAAGCTGCTGGCGTCGCTTAGCTCGATTCTCCTTTATTGGTATCACTACAGCCACAACGGCGAACGCATc                                                                       <  2:1126556/140‑1 (MQ=255)                                                                  gcgcTCGGCTGCACGCTGCCAGAAAAAGAGGGGCATACCGTCTCTGGCGCGCGGGATATTGCCGACAAGCTGCTGGCGTCGCTTAGCTCGATTCTCCTTTATTGGTATCACTACAGCCACAACgg                                                                                 >  2:1263280/1‑125 (MQ=255)                                                                  gcgcTCGGCTGCACGCTGCCAGAAAAAGAGGGGCATACCGTCTCTGGCGCGCGGGATATTGCCGACAAGCTGCTGGCGTCGCTTAGCTCGATTCTCCTTTATTGGTATCACTACAGCCACAACgg                                                                                 <  1:1263280/125‑1 (MQ=255)                                                                  gcgcTCGGCTGCACGCTGCCAGAAAAAGAGGGGCATACCGTCTCTGGCGCGCGGGATATTGCCGACAAGCTGCTGGCGTCGCTTAGCTCGATTCTCCTTTATTGGTATCACTACAGCCACAACGGCGAACGCATCCAAcc                                                                  >  1:366014/1‑140 (MQ=255)                                                                  gcgcTCGGCTGCACGCTGCCAGAAAAAGAGGGGCATACCGTCTCTGGCGCGCGGGATATTGCCGACAAGCTGCTGGCGTCGCTTAGCTCGATTCTCCTTTATTGGTATCACTACAGCCACAACGGCGAACGCATCCAAcc                                                                  <  2:754779/140‑1 (MQ=255)                                                                     cTCGGCTGCACGCTGCCAGAAAAAGAGGGGCATACCGTCTCTGGCGCGCGGGATATTGCCGACAAGCTGCTGGCGTCGCTTAGCTCGATTCTCCTTTATTGGTATCACTACAGCCACAACGGCGAACGCATCCAACCGGa                                                               >  2:917936/1‑140 (MQ=255)                                                                            gCACGCTGCCAGAAAAAGAGGGGCATACCGTCTCTGGCGCGCGGGATATTGCCGACAAGCTGCTGGCGTCGCTTAGCTCGATTCTCCTTTATTGGTATCACTACAGCCACAACGGCGAACGCATCCAACCGGAAACCGAt                                                        <  2:770807/140‑1 (MQ=255)                                                                                    ccAGAAAAAGAGGGGCATACCGTCTCTGGCGCGCGGGATATTGCCGACAAGCTGCTGGCGTCGCTTAGCTCGATTATCCTTTATTGGTATCACTACAGCCACAACGGCGAACGCATCCAACCGGAAACCGATGACGACTc                                                >  2:1132476/1‑140 (MQ=255)                                                                                                  gCATACCGTCTCTGGCGCGCGGGATATTGCCGACAAGCTGCTGGCGTCGCTTAGCTCGATTCTCCTTTATTGGTATCACTACAGCCACAACGGCGAACGCATCCAACCGGAAACCGATGACGACTCCATCGGCGGTcccc                                  >  2:261496/1‑137 (MQ=255)                                                                                                     tACCGTCTCTGGCGCGCGGGATATTGCCGACAAGCTGCTGGCGTCGCTTAGCTCGATTCTCCTTTATTGGTATCACTACAGCCACAACGGCGAACGCATCCAACCGGAAACCGATGACGACTCCATCGGCGGTCACTTcc                               >  2:1087288/1‑140 (MQ=255)                                                                                                      aCCGTCTCTGGCGCGCGGGATATTGCCGACAAGCTGCTGGCGTCGCTTAGCTCGATTCTCCTTTATTGGTATCACTACAGCCACAACGGCGAACGCATCCAACCGGAAACCGATGACGACTCCATCGGCGGTCACTTCCt                              <  1:207205/140‑1 (MQ=255)                                                                                                        cGTCTCTGGCGCGCGGGATATTGCCGACAAGCTGCTGGCGTCGCTTAGCTCGATTCTCCTTTATTGGTATCACTACAGCCACAACGGCGAACGCATCCAACCGGAAACCGATGACGACTCCATCGGCGGTCACTTCCTGc                            <  2:1046716/140‑1 (MQ=255)                                                                                                        cGTCTCTGGCGCGCGGGATATTGCCGACAAGCTGCTGGCGTCGCTTAGCTCGATTCTCCTTTATTGGTATCACTACAGCCACAACGGCGAACGCATCCAACCGGAAACCGATGACGACTCCATCGGCGGTCACTTCCTGc                            >  1:1106037/1‑140 (MQ=255)                                                                                                          tctcTGGCGCGCGGGATATTGCCGACAAGCTGCTGGCGTCGCTTAGCTCGATTCTCCTTTATTGGTATCACTACAGCCACAACGGCGAACGCATCCAACCGGAAACCGATGACGACTCCATCGGCGGTCACTTCCTGCAt                          >  2:1401443/1‑140 (MQ=255)                                                                                                                gcgcgcGGGATATTGCCGACAAGCTGCTGGCGTCGCTTAGCTCGATTCTCCTTTATTGGTATCACTACAGCCACAACGGCGAACGCATCCAACCGGAAACCGATGACGACTCCATCGGCGGTCACTTCCTGCATctgctg                    >  2:182710/1‑140 (MQ=255)                                                                                                                   cgcgGGATATTGCCGACAAGCTGCTGGCGTCGCTTAGCTCGATTCTCCTTTATTGGTATCACTACAGCCACAACGGCGAACGCATCCAACCGGAAACCGATGACGACTCCATCGGCGGTCACTTCCTGCATCTGCTGCAc                 <  1:1295845/140‑1 (MQ=255)                                                                                                                   cgcgGGATATTGCCGACAAGCTGCTGGCGTCGCTTAGCTCGATTCTCCTTTATTGGTATCACTACAGCCACAACGGCGAACGCATCCAACCGGAAACCGATGACGACTCCATCGGCGGTCACTTCCTGCATCTGCTGCAc                 <  1:917936/140‑1 (MQ=255)                                                                                                                     cgGGATATTGCCGACAAGCTGCTGGCGTCGCTTAGCTCGATTCTCCTTTATTGGTATCACTACAGCCACAACGGCGAACGCATCCAACCGGAAACCGATGACGACTCCATCGGCGGTCACTTCCTGCATCTGCTGCACgg               >  1:1123475/1‑140 (MQ=255)                                                                                                                                  aCAAGCTGCTGGCGTCGCTTAGCTCGATTCTCCTTTATTGGTATCACTACAGCCACAACGGCGAACGCATCCAACCGGAAACCGATGACGACTCCATCGGCGGTCACTTCCTGCATCTGCTGCACGGCGAAAAGCCATCg  >  1:280136/1‑140 (MQ=255)                                                                                                                                  aCAAGCTGCTGGCGTCGCTTAGCTCGATTCTCCTTTATTGGTATCACTACAGCCACAACGGCGAACGCATCCAACCGGAAACCGATGACGACTCCATCGGCGGTCACTTCCTGCATCTGCTGCACGGCGAAAAGCCATCg  <  1:578060/140‑1 (MQ=255)                                                                                                                                      |                                                                                                                                        GTACCGTGCTGGAAGCCTTACCGGCGGCGTCGCACCCGATGGATGTTATGCGCACCGGTGTTTCCGCGCTCGGCTGCACGCTGCCAGAAAAAGAGGGGCATACCGTCTCTGGCGCGCGGGATATTGCCGACAAACTGCTGGCGTCGCTTAGCTCGATTCTCCTTTATTGGTATCACTACAGCCACAACGGCGAACGCATCCAACCGGAAACCGATGACGACTCCATCGGCGGTCACTTCCTGCATCTGCTGCACGGCGAAAAGCCATCG  >  NC_000913/350292‑350560

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑