Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,840,211	T→C	C230R (TGC→CGC)	ascF →	beta‑glucoside specific PTS enzyme IIBC component

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,840,211	0	T	C	100.0%	286.7 / NA	78	C230R (TGC→CGC)	ascF	beta‑glucoside specific PTS enzyme IIBC component
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (43/35);  total (43/35)

CGGGTGTGCTGGTACATCCGAGTTTTATTGAACTGATGGCGAAAGCGGCCCAGGGTGAACATGTCGAATTTGCCCTGATTCCGGTCACCGCGGTGAAATACACCTACACGGTGATCCCGGCGCTGGTGATGACCTGGTGCCTGTCATATATCGAACGCTGGGTGGACAGCATTACACCGGCGGTGACCAAAAACTTCCTCAAACCAATGCTGATTGTGTTGATTGCCGCACCGCTGGCAATCCTGCTGATTGGCCCGATTGGTATCTGGA  >  NC_000913/2840074‑2840343                                                                                                                                          |                                                                                                                                     cgGGTGTGCTGGTACATCCGAGTTTTATTGAACTGATGGCGAAAGCGGCCCAGGGTGAACATGTCGAATTTGCCCTGATTCCGGTCACCGCGGTGAAATACACCTACACGGTGATCCCGGCGCTGGTGATGACCTGgcgc                                                                                                                                    <  2:289145/140‑1 (MQ=255) cgGGTGTGCTGGTACATCCGAGTTTTATTGAACTGATGGCGAAAGCGGCCCAGGGTGAACATGTCGAATTTGCCCTGATTCCGGTCACCGCGGTGAAATACACCTACACGGTGATCCCGGCGCTGGTGATGACCTGgcgc                                                                                                                                    <  2:183004/140‑1 (MQ=255) cgGGTGTGCTGGTACATCCGAGTTTTATTGAACTGATGGCGAAAGCGGCCCAGGGTGAACATGTCGAATTTGCCCTGATTCCGGTCACCGCGGTGAAATACACCTACACGGTGATCCCGGCGCTGGTGATGACCTGgcgc                                                                                                                                    <  2:774744/140‑1 (MQ=255)        gCTGGTACATCCGAGTTTTATTGAACTGATGGCGAAAGCGGCCCAGGGTGAACATGTCGAATTTGCCCTGATTCCGGTCACCGCGGTGAAATACACCTACACGGTGATCCCGGCGCTGGTGATGACCTGGCGCCTGTCat                                                                                                                             <  2:1322953/140‑1 (MQ=255)                 tCCGAGTTTTATTGAACTGATGGCGAAAGCGGCCCAGGGTGAACATGTCGAATTTGCCCTGATTCCGGTCACCGCGGTGAAATACACCTACACGGTGATCCCGGCGCTGGTGATGACCTGgcgc                                                                                                                                    >  2:666689/1‑124 (MQ=255)                 tCCGAGTTTTATTGAACTGATGGCGAAAGCGGCCCAGGGTGAACATGTCGAATTTGCCCTGATTCCGGTCACCGCGGTGAAATACACCTACACGGTGATCCCGGCGCTGGTGATGACCTGGCGCCt                                                                                                                                  >  1:1256294/1‑126 (MQ=255)                 tCCGAGTTTTATTGAACTGATGGCGAAAGCGGCCCAGGGTGAACATGTCGAATTTGCCCTGATTCCGGTCACCGCGGTGAAATACACCTACACGGTGATCCCGGCGCTGGTGATGACCTGGCGCCt                                                                                                                                  <  2:1256294/126‑1 (MQ=255)                 tCCGAGTTTTATTGAACTGATGGCGAAAGCGGCCCAGGGTGAACATGTCGAATTTGCCCTGATTCCGGTCACCGCGGTGAAATACACCTACACGGTGATCCCGGCGCTGGTGATGACCTGGCGCCTGTCATATATCGAAc                                                                                                                    >  2:246533/1‑140 (MQ=255)                 tCCGAGTTTTATTGAACTGATGGCGAAAGCGGCCCAGGGTGAACATGTCGAATTTGCCCTGATTCCGGTCACCGCGGTGAAATACACCTACACGGTGATCCCGGCGCTGGTGATGACCTGGCGCCTGTCATATATCGAAc                                                                                                                    >  2:792610/1‑140 (MQ=255)                  ccGAGTTTTATTGAACTGATGGCGAAAGCGGCCCAGGGTGAACATGTCGAATTTGCCCTGATTCCGGTCACCGCGGTGAAATACACCTACACGGTGATCCCGGCGCTGGTGATGACCTGGCGCCTGTCATATATCGAACg                                                                                                                   <  1:837890/140‑1 (MQ=255)                  ccGAGTTTTATTGAACTGATGGCGAAAGCGGCCCAGGGTGAACATGTCGAATTTGCCCTGATTCCGGTCACCGCGGTGAAATACACCTACACGGTGATCCCGGCGCTGGTGATGACCTGGCGCCTGTCATATATCGAACg                                                                                                                   >  2:388083/1‑140 (MQ=255)                   cGAGTTTTATTGAACTGATGGCGAAAGCGGCCCAGGGTGAACATGTCGAATTTGCCCTGATTCCGGTCACCGCGGTGAAATACACCTACACGGTGATCCCGGCGCTGGTGATGACCTGGCGCCTGTCATATATCGAACGc                                                                                                                  >  1:669739/1‑140 (MQ=255)                      gTTTTATTGAACTGATGGCGAAAGCGGCCCAGGGTGAACATGTCGAATTTGCCCTGATTCCGGTCACCGCGGTGAAATACACCTACACGGTGATCCCGGCGCTGGTGATTACCTGGCGCCTGTCATATATCGAACGCTgg                                                                                                               >  1:1177726/1‑140 (MQ=255)                           aTTGAACTGATGGCGAAAGCGGCCCAGGGTGAACATGTCGAATTTGCCCTGATTCCGGTCACCGCGGTGAAATACACCTACACGGTGATCCCGGCGCTGGTGATGACCTGGCGCCTGTCATATATCGAACGCTGGGTGGa                                                                                                          <  1:675192/140‑1 (MQ=255)                           aTTGAACTGATGGCGAAAGCGGCCCAGGGTGAACATGTCGAATTTGCCCTGATTCCGGTCACCGCGGTGAAATACACCTACACGGTGATCCCGGCGCTGGTGATGACCTGGCGCCTGTCATATATCGAACGCTGGGTGGa                                                                                                          <  1:326863/140‑1 (MQ=255)                           aTTGAACTGATGGCGAAAGCGGCCCAGGGTGAACATGTCGAATTTGCCCTGATTCCGGTCACCGCGGTGAAATACACCTACACGGTGATCCCGGCGCTGGTGATGACCTGGCGCCTGTCATATATCGAACGCTGGGTGGa                                                                                                          <  2:450071/140‑1 (MQ=255)                           aTTGAACTGATGGCGAAAGCGGCCCAGGGTGAACATGTCGAATTTGCCCTGATTCCGGTCACCGCGGTGAAATACACCTACACGGTGATCCCGGCGCTGGTGATGACCTGGCGCCTGTCATATATCGAACGCTGGGTGGa                                                                                                          <  2:1337728/140‑1 (MQ=255)                                    aTGGCGAAAGCGGCCCAGGGTGAACATGTCGAATTTGCCCTGATTCCGGTCACCGCGGTGAAATACACCTACACGGTGATCCCGGCGCTGGTGATGACCTGGCGCCTGTCATATATCGAACGCTGGGTGGACAGCATTac                                                                                                 >  2:498544/1‑140 (MQ=255)                                    aTGGCGAAAGCGGCCCAGGGTGAACATGTCGAATTTGCCCTGATTCCGGTCACCGCGGTGAAATACACCTACACGGTGATCCCGGCGCTGGTGATGACCTGGCGCCTGTCATATATCGAACGCTGGGTGGACAGCATTac                                                                                                 >  1:1402580/1‑140 (MQ=255)                                            aGCGGCCCAGGGTGAACATGTCGAATTTGCCCTGATTCCGGTCACCGCGGTGAAATACACCTACACGGTGATCCCGGCGCTGGTGATGACCTGGCGCCTGTCATATATCGAACGCTGGGTGGACAGCATTACACcggcgg                                                                                         >  2:436552/1‑140 (MQ=255)                                                 cccAGGGTGAACATGTCGAATTTGCCCTGATTCCGGTCACCGCGGTGAAATACACCTACACGGTGATCCCGGCGCTGGTGATGACCTGGCGCCTGTCATATATCGAACGCTGGGTGGACAGCATTACACCGGCGGTGAcc                                                                                    >  2:1118862/1‑140 (MQ=255)                                                           aCATGTCGAATTTGCCCTGATTCCGGTCACCGCGGTGAAATACACCTACACGGTGATCCCGGCGCTGGTGATGACCTGGCGCCTGTCATATATCGAACGCTGGGTGGACAGCATTACACCGGCGGTGACCAAAAACTTcc                                                                          >  1:955893/1‑140 (MQ=255)                                                               gTCGAATTTGCCCTGATTCCGGTCACCGCGGTGAAATACACCTACACGGTGATCCCGGCGCTGGTGATGACCTGGCGCCTGTCATATATCGAACGCTGGGTGGACAGCATTACACCGGCGGTGACCAAAAACTTCCTCaa                                                                      >  1:1059693/1‑140 (MQ=255)                                                               gTCGAATTTGCCCTGATTCCGGTCACCGCGGTGAAATACACCTACACGGTGATCCCGGCGCTGGTGATGACCTGGCGCCTGTCATATATCGAACGCTGGGTGGACAGCATTACACCGGCGGTGACCAAAAACTTCCTCaa                                                                      >  1:899457/1‑140 (MQ=255)                                                               gTCGAATTTGCCCTGATTCCGGTCACCGCGGTGAAATACACCTACACGGTGATCCCGGCGCTGGTGATGACCTGGCGCCTGTCATATATCGAACGCTGGGTGGACAGCATTACACCGGCGGTGACCAAAAACTTCCTCaa                                                                      >  2:906968/1‑140 (MQ=255)                                                               gTCGAATTTGCCCTGATTCCGGTCACCGCGGTGAAATACACCTACACGGTGATCCCGGCGCTGGTGATGACCTGGCGCCTGTCATATATCGAACGCTGGGTGGACAGCATTACACCGGCGGTGACCAAAAACTTCCTCaa                                                                      >  1:1420954/1‑140 (MQ=255)                                                                 cGAATTTGCCCTGATTCCGGTCACCGCGGTGAAATACACCTACACGGTGATCCCGGCGCTGGTGATTACCTGGCGCCTGTCATATATCGAACGCTGGGTGGACAGCATTACACCGGCGGTGACCAAAAACTTCCTCAAAc                                                                    <  2:1177726/140‑1 (MQ=255)                                                                 cGAATTTGCCCTGATTCCGGTCACCGCGGTGAAATACACCTACACGGTGATCCCGGCGCTGGTGATGACCTGGCGCCTGTCATATATCGAACGCTGGGTGGACAGCATTACCCCGGCGGTGACCAAAAACTTCCTCAAAc                                                                    <  2:374884/140‑1 (MQ=255)                                                                  gAATTTGCCCTGATTCCGGTCACCGCGGTGAAATACACCTACACGGTGATCCCGGCGCTGGTGATGACCTGGCGCCTGTCATATATCGAACGCTGGGTGGACAGCATTACACCGGCGGTGACCAAAAACTTCCTCAAAcc                                                                   <  1:418071/140‑1 (MQ=255)                                                                         cccTGATTCCGGTCACCGCGGTGAAATACACCTACACGGTGATCCCGGCGCTGGTGATGACCTGGCGCCTGTCATATATCGAACGCTGGGTGGACAGCATTACACCGGCGGTGACCAAAAACTTCCTCAAACCAATGCTg                                                            >  2:202865/1‑140 (MQ=255)                                                                                 ccGGTCACCGCGGTGAAATACACCTACACGGTGATCCCGGCGCTGGTGATGACCTGGCGCCTGTCATATATCGAACGCTGGGTGGACAGCATTACACCGGCGGTGACCAAAAACTTCCTCAAACCAATGCTGATTGTGtt                                                    <  2:669739/140‑1 (MQ=255)                                                                                  cGGTCACCGCGGTGAAATACACCTACACGGTGATCCCGGCGCTGGTGATGACCTGGCGCCTGTCATATATCGAACGCTGGGTGGACAGCATTACACCGGCGGTGACCAAAAACTTCCTCAAACCAATGCTGATTGTGTTg                                                   >  2:388503/1‑140 (MQ=255)                                                                                    gTCACCGCGGTGAAATACACCTACACGGTGATCCCGGCGCTGGTGATGACCTGGCGCCTGTCATATATCGAACGCTGGGTGGACAGCATTACACCGGCGGTGACCAAAAACTTCCTCAAACCAATGCTGATTGTGTTGAt                                                 >  1:1203553/1‑140 (MQ=255)                                                                                    gTCACCGCGGTGAAATACACCTACACGGTGATCCCGGCGCTGGTGATGACCTGGCGCCTGTCATATATCGAACGCTGGGTGGACAGCATTACACCGGCGGTGACCAAAAACTTCCTCAAACCAATGCTGATTGTGTTGAt                                                 <  1:388083/140‑1 (MQ=255)                                                                                        ccGCGGTGAAATACACCTACACGGTGATCCCGGCGCTGGTGATGACCTGGCGCCTGTCATATATCGAACGCTGGGTGGACAGCATTACACCGGCGGTGACCAAAAACTTCCTCAAACCAATGCTGATTGTGTTGATTGcc                                             >  2:716032/1‑140 (MQ=255)                                                                                        ccGCGGTGAAATACACCTACACGGTGATCCCGGCGCTGGTGATGACCTGGCGCCTGTCATATATCGAACGCTGGGTGGACAGCATTACACCGGCGGTGACCAAAAACTTCCTCAAACCAATGCTGATTGTGTTGATTGcc                                             >  1:114245/1‑140 (MQ=255)                                                                                        ccGCGGTGAAATACACCTACACGGTGATCCCGGCGCTGGTGATGACCTGGCGCCTGTCATATATCGAACGCTGGGTGGACAGCATTACACCGGCGGTGACCAAAAACTTCCTCAAACCAATGCTGATTGTGTTGATTGcc                                             >  2:94387/1‑140 (MQ=255)                                                                                         cgcgGTGAAATACACCTACACGGTGATCCCGGCGCTGGTGATGACCTGGCGCCTGTCATATATCGAACGCTGGGTGGACAGCATTACACCGGCGGTGACCAAAAACTTCCTCAAACCAATGCTGATTGTGTTGATTGCCg                                            <  2:924539/140‑1 (MQ=255)                                                                                         cgcgGTGAAATACACCTACACGGTGATCCCGGCGCTGGTGATGACCTGGCGCCTGTCATATATCGAACGCTGGGTGGACAGCATTACACCGGCGGTGACCAAAAACTTCCTCAAACCAATGCTGATTGTGTTGATTGCCg                                            >  2:1312306/1‑140 (MQ=255)                                                                                         cgcgGTGAAATACACCTACACGGTGATCCCGGCGCTGGTGATGACCTGGCGCCTGTCATATATCGAACGCTGGGTGGACAGCATTACACCGGCGGTGACCAAAAACTTCCTCAAACCAATGCTGATTGTGTTGATTGCCg                                            >  1:1038434/1‑140 (MQ=255)                                                                                             gTGAAATACACCTACACGGTGATCCCGGCGCTGGTGATGACCTGGCGCCTGTCATATATCGAACGCTGGGTGGACAGCATTACACCGGCGGTGACCAAAAACTTCCTCAAACCAATGCTGATTGTGTTGATTGCCGCa                                          >  2:300504/1‑138 (MQ=255)                                                                                             gTGAAATACACCTACACGGTGATCCCGGCGCTGGTGATGACCTGGCGCCTGTCATATATCGAACGCTGGGTGGACAGCATTACACCGGCGGTGACCAAAAACTTCCTCAAACCAATGCTGATTGTGTTGATTGCCGCa                                          <  1:300504/138‑1 (MQ=255)                                                                                             gTGAAATACACCTACACGGTGATCCCGGCGCTGGTGATGACCTGGCGCCTGCCATATATCGAACGCTGGGTGGACAGCATTACACCGGCGGTGACCAAAAACTTCCTCAAACCAATGCTGATTGTGTTGATTGCCGCa                                          <  1:1187253/138‑1 (MQ=255)                                                                                             gTGAAATACACCTACACGGTGATCCCGGCGCTGGTGATGACCTGGCGCCTGCCATATATCGAACGCTGGGTGGACAGCATTACACCGGCGGTGACCAAAAACTTCCTCAAACCAATGCTGATTGTGTTGATTGCCGCa                                          >  2:1187253/1‑138 (MQ=255)                                                                                                  aTACACCTACACGGTGATCCCGGCGCTGGTGATGACCTGGCGCCTGTCATATATCGAACGCTGGGTGGACAGCATTACACCGGCGGTGACCAAAAACTTCCTCAAACCAATGCTGATTGTGTTGATTGCCGCACCGCTgg                                   <  1:906968/140‑1 (MQ=255)                                                                                                    acacCTACACGGTGATCCCGGCGCTGGTGATGACCTGGCGCCTGTCATATATCGAACGCTGGGTGGACAGCATTACACCGGCGGTGACCAAAAACTTCCTCAAACCAATGCTGATTGTGTTGATTGCCGCACCGCTGGCa                                 <  1:716032/140‑1 (MQ=255)                                                                                                          acacGGTGATCCCGGCGCTGGTGATGACCTGGCGCCTGTCATATATCGAACGCTGGGTGGACAGCATTACACCGGCGGTGACCAAAAACTTCCTCAAACCAATGCTGATTGTGTTGATTGCCGCACCGCTGGCAATCctg                           >  1:224522/1‑140 (MQ=255)                                                                                                          acacGGTGATCCCGGCGCTGGTGATGACCTGGCGCCTGTCATATATCGAACGCTGGGTGGACAGCATTACACCGGCGGTGACCAAAAACTTCCTCAAACCAATGCTGATTGTGTTGATTGCCGCACCGCTGGCAATCctg                           >  2:452896/1‑140 (MQ=255)                                                                                                          acacGGTGATCCCGGCGCTGGTGATGACCTGGCGCCTGTCATATATCGAACGCTGGGTGGACAGCATTACACCGGCGGTGACCAAAAACTTCCTCAAACCAATGCTGATTGTGTTGATTGCCGCACCGCTGGCAATCctg                           >  1:899012/1‑140 (MQ=255)                                                                                                           cacGGTGATCCCGGCGCTGGTGATGACCTGGCGCCTGTCATATATCGAACGCTGGGTGGACAGCATTACACCGGCGGTGACCAAAAACTTCCTCAAACCAATGCTGATTGTGTTGATTGCCGCACCGCTGGCAATCctgc                          >  2:994987/1‑140 (MQ=255)                                                                                                           cacGGTGATCCCGGCGCTGGTGATGACCTGGCGCCTGTCATATATCGAACGCTGGGTGGACAGCATTACACCGGCGGTGACCAAAAACTTCCTCAAACCAATGCTGATTGTGTTGATTGCCGCACCGCTGGCAATCctgc                          >  1:434200/1‑140 (MQ=255)                                                                                                           cacGGTGATCCCGGCGCTGGTGATGACCTGGCGCCTGTCATATATCGAACGCTGGGCGGACAGCATTACACCGGCGGTGACCAAAAACTTCCTCAAACCAATGCTGATTGTGTTGATTGCCGCACCGCTGGCAATCctgc                          >  2:918358/1‑140 (MQ=255)                                                                                                             cGGTGATCCCGGCGCTGGTGATGACCTGGCGCCTGTCATATATCGAACGCTGGGTGGACAGCATTACACCGGCGGTGACCAAAAACTTCCTCAAACCAATGCTGATTGTGTTGATTGCCGCACCGCTGGCAATCctgctg                        >  1:245917/1‑140 (MQ=255)                                                                                                             cGGTGATCCCGGCGCTGGTGATGACCTGGCGCCTGTCATATATCGAACGCTGGGTGGACAGCATTACACCGGCGGTGACCAAAAACTTCCTCAAACCAATGCTGATTGTGTTGATTGCCGCACCGCTGGCAATCctgctg                        >  1:809081/1‑140 (MQ=255)                                                                                                             cGGTGATCCCGGCGCTGGTGATGACCTGGCGCCTGTCATATATCGAACGCTGGGTGGACAGCATTACACCGGCGGTGACCAAAAACTTCCTCAAACCAATGCTGATTGTGTTGATTGCCGCACCGCTGGCAATCctgctg                        >  2:286798/1‑140 (MQ=255)                                                                                                              ggTGATCCCGGCGCTGGTGATGACCTGGCGCCTGTCATATATCGAACGCTGGGTGGACAGCATTACACCGGCGGTGACCAAAAACTTCCTCAAACCAATGCTGATTGTGTTGATTGCCGCACCGCTGGCAATCCTGCTGa                       <  1:1118862/140‑1 (MQ=255)                                                                                                                tGATCCCGGCGCTGGTGATGACCTGGCGCCTGTCATATATCGAACGCTGGGTGGACAGCATTACACCGGCGGTGACCAAAAACTTCCTCAAACCAATGCTGATTGTGTTGATTGCCGCACCGCTGGCAATCCTGCTGAtt                     >  1:1115740/1‑140 (MQ=255)                                                                                                                  aTCCCGGCGCTGGTGATGACCTGGCGCCTGTCATATATCGAACGCTGGGTGGACAGCATTACACCGGCGGTGACCAAAAACTTCCTCAAACCAATGCTGATTGTGTTGATTGCCGCACCGCTGGCAATCct                            >  2:82714/1‑131 (MQ=255)                                                                                                                  aTCCCGGCGCTGGTGATGACCTGGCGCCTGTCATATATCGAACGCTGGGTGGACAGCATTACACCGGCGGTGACCAAAAACTTCCTCAAACCAATGCTGATTGTGTTGATTGCCGCACCGCTGGCAATCct                            <  1:82714/131‑1 (MQ=255)                                                                                                                  aTCCCGGCGCTGGTGATGACCTGGCGCCTGTCATATATCGAACGCTGGGTGGACAGCATTACACCGGCGGTGACCAAAAACTTCCTCAAACCAATGCTGATTGTGTTGATTGCCGCACCGCTGGCAATCCTGCTGATTgg                   >  1:464056/1‑140 (MQ=255)                                                                                                                    cccGGCGCTGGTGATGACCTGGCGCCTGTCATATATCGAACGCTGGGTGGACAGCATTACACCGGCGGTGACCAAAAACTTCCTCAAACCAATGCTGATTGTGTTGATTGCCGCACCGCTGGCAATCCTGCTGATTGGcc                 <  2:1038434/140‑1 (MQ=255)                                                                                                                    cccGGCGCTGGTGATGACCTGGCGCCTGTCATATATCGAACGCTGGGTGGACAGCATTACACCGGCGGTGACCAAAAACTTCCTCAAACCAATGCTGATTGTGTTGATTGCCGCACCGCTGGCAATCCTGCTGATTGGcc                 <  1:961708/140‑1 (MQ=255)                                                                                                                    cccGGCGCTGGTGATGACCTGGCGCCTGTCATATATCGAACGCTGGGTGGACAGCATTACACCGGCGGTGACCAAAAACTTCCTCAAACCAATGCTGATTGTGTTGATTGCCGCACCGCTGGCAATCCTGCTGATTGGcc                 <  1:429992/140‑1 (MQ=255)                                                                                                                     ccGGCGCTGGTGATGACCTGGCGCCTGTCATATATCGAACGCTGGGTGGACAGCATTACACCGGCGGTGACCAAAAACTTCCTCAAACCAATGCTGATTGTGTTGATTGCCGCACCGCTGGCAATCCTGCTGATTGGccc                >  1:732753/1‑140 (MQ=255)                                                                                                                      cGGCGCTGGTGATGACCTGGCGCCTGTCATATATCGAACGCTGGGTGGACAGCATTACACCGGCGGTGACCAAAAACTTCCTCAAACCAATGCTGATTGTGTTGATTGCCGCACCGCTGGCAATCCTGCTGATTGGcc                 <  1:217700/138‑1 (MQ=255)                                                                                                                      cGGCGCTGGTGATGACCTGGCGCCTGTCATATATCGAACGCTGGGTGGACAGCATTACACCGGCGGTGACCAAAAACTTCCTCAAACCAATGCTGATTGTGTTGATTGCCGCACCGCTGGCAATCCTGCTGATTGGcc                 >  2:217700/1‑138 (MQ=255)                                                                                                                        gcgcTGGTGATGACCTGGCGCCTGTCATATATCGAACGCTGGGTGGACAGCATTACACCGGCGGTGACCAAAAACTTCCTCAAACCAATGCTGATTGTGTTGATTGCCGCACCGCTGGCAATCCTGCTGATTGGc                  >  2:460397/1‑135 (MQ=255)                                                                                                                        gcgcTGGTGATGACCTGGCGCCTGTCATATATCGAACGCTGGGTGGACAGCATTACACCGGCGGTGACCAAAAACTTCCTCAAACCAATGCTGATTGTGTTGATTGCCGCACCGCTGGCAATCCTGCTGATTGGc                  <  1:460397/135‑1 (MQ=255)                                                                                                                        gcgcTGGTGATGACCTGGCGCCTGTCATATATCGAACGCTGGGTGGACAGCATTACACCGGCGGTGACCAAAAACTTCCTCAAACCAATGCTGATTGTGTTGATTGCCGCACCGCTGGCAATCCTGCTGATTGGTCCGAt             <  1:94387/140‑1 (MQ=255)                                                                                                                        gcgcTGGTGATGACCTGGCGCCTGTCATATATCGAACGCTGGGTGGACAGCATTACACCGGCGGTGACCAAAAACTTCCTCAAACCAATGCTGATTGTGTTGATTGCCGCACCGCTGGCAATCCTGCTGATTGGCCCGAt             <  2:1420954/140‑1 (MQ=255)                                                                                                                        gcgcTGGTGATGACCTGGCGCCTGTCATATATCGAACGCTGGGTGGACAGCATTACACCGGCGGTGACCAAAAACTTCCTCAAACCAATGCTGATTGTGTTGATTGCCGCACCGCTGGCAATCCTGCTGATTGGCCCGAt             <  2:1149479/140‑1 (MQ=255)                                                                                                                        gcgcTGGTGATGACCTGGCGCCTGTCATATATCGAACGCTGGGTGGACAGCATTACACCGGCGGTGACCAAAAACTTCCTCAAACCAATGCTGATTGTGTTGATTGCCGCACCGCTGGCAATCCTGCTGATTGGCCCGAt             <  1:246533/140‑1 (MQ=255)                                                                                                                         cgcTGGTGATGACCTGGCGCCTGTCATATATCGAACGCTGGGTGGACAGCATTACACCGGCGGTGACCAAAAACTTCCTCAAACCAATGCTGATTGTGTTGATTGCCGCACCGCTGGCAATCCTGCTGATTGGCCCGAtt            <  1:1312306/140‑1 (MQ=255)                                                                                                                         cgcTGGTGATGACCTGGCGCCTGTCATATATCGAACGCTGGGTGGACAGCATTACACCGGCGGTGACCAAAAACTTCCTCAAACCAATGCTGATTGTGTTGATTGCCGCACCGCTGGCAATCCTGCTGATTGGCCCGAtt            <  2:809081/140‑1 (MQ=255)                                                                                                                           cTGGTGATGACCTGGCGCCTGTCATATATCGAACGCTGGGTGGACAGCATTACACCGGCGGTGACCAAAAACTTCCTCAAACCAATGCTGATTGTGTTGATTGCCGCACCGCTGGCAATCCTGCTGATTGGCCCGATTgg          <  1:498544/140‑1 (MQ=255)                                                                                                                            tGGTGATGACCTGGCGCCTGTCATATATCGAACGCTGGGTGGACAGCATTACACCGGCGGTGACCAAAAACTTCCTCAAACCAATGCTGATTGTGTTGATTGCCGCACCGCTGGCAATCCTGCTGATTGGCCCGATTGGt         >  1:731164/1‑140 (MQ=255)                                                                                                                            tGGTGATGACCTGGCGCCTGTCATATATCGAACGCTGGGTGGACAGCATTACACCGGCGGTGACCAAAAACTTCCTCAAACCAATGCTGATTGTGTTGATTGCCGCACCGCTGGCAATCCTGCTGATTGGCCCGATTGGt         <  2:899457/140‑1 (MQ=255)                                                                                                                            tGGTGATGACCTGGCGCCTGTCATATATCGAACGCTGGGTGGACAGCATTACACCGGCGGTGACCAAAAACTTCCTCAAACCAATGCTGATTGTGTTGATTGCCGCACCGCTGGCAATCCTGCTGATTGGCCCGATTGGt         <  2:1059693/140‑1 (MQ=255)                                                                                                                             ggTGATGACCTGGCGCCTGTCATATATCGAACGCTGGGTGGACAGCATTACACCGGCGGTGACCAAAAACTTCCTCAAACCAATGCTGATTGTGTTGATTGCCGCACCGCTGGCAATCCTGCTGATTGGCCCGATTGGTa        <  1:994987/140‑1 (MQ=255)                                                                                                                              gTGATGACCTGGCGCCTGTCATATATCGAACGCTGGGTGGACAGCATTACACCGGCGGTGACCAAAAACTTCCTCAAACCAATGCTGATTGTGTTGATTGCCGCACCGCTGGCAATCCTGCTGATTGGCCCGAt             >  1:1333934/1‑134 (MQ=255)                                                                                                                              gTGATGACCTGGCGCCTGTCATATATCGAACGCTGGGTGGACAGCATTACACCGGCGGTGACCAAAAACTTCCTCAAACCAATGCTGATTGTGTTGATTGCCGCACCGCTGGCAATCCTGCTGATTGGCCCGAt             <  2:1333934/134‑1 (MQ=255)                                                                                                                                   gaCCTGGCGCCTGTCATATATCGAACGCTGGGTGGACAGCATTACACCGGCGGTGACCAAAAACTTCCTCAAACCAATGCTGATTGTGTTGATTGCCGCACCGCTGGCAATCCTGCTGATTGGCCCGATTGGTATCTGGa  >  2:998493/1‑140 (MQ=255)                                                                                                                                          |                                                                                                                                     CGGGTGTGCTGGTACATCCGAGTTTTATTGAACTGATGGCGAAAGCGGCCCAGGGTGAACATGTCGAATTTGCCCTGATTCCGGTCACCGCGGTGAAATACACCTACACGGTGATCCCGGCGCTGGTGATGACCTGGTGCCTGTCATATATCGAACGCTGGGTGGACAGCATTACACCGGCGGTGACCAAAAACTTCCTCAAACCAATGCTGATTGTGTTGATTGCCGCACCGCTGGCAATCCTGCTGATTGGCCCGATTGGTATCTGGA  >  NC_000913/2840074‑2840343

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑