Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,941,867	T→C	Q234R (CAG→CGG)	gcvA ←	DNA‑binding transcriptional dual regulator GcvA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,941,867	0	T	C	100.0%	365.2 / NA	97	Q234R (CAG→CGG)	gcvA	DNA‑binding transcriptional dual regulator GcvA
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (53/44);  total (53/44)

TACCCAGTTCTGCCTGACTGTCATGACAAACCAGATAAAAAGCATTTTTACTGACCAGAACATCATTAAACGGGCAAACAAGACGTCCGGCCTCGATTTCAGATTGCGCCATCACGTTATTTGCCAGCGCCACTCCCTGCCCGTGGATAGCCGCTTGCAGCACCATGGCGCTATGGCTAAAAATTGGCCCTTGCTGAACGTTGATATGATTTAACCCCAACTGTCGGGTATATGTCTGCCAGTCACGGCGCGAAGCATCATGTAATAACG  >  NC_000913/2941730‑2941999                                                                                                                                          |                                                                                                                                     tACCCAGTTCTGCCTGACTGTCATGACAAACCAGATAAAAAGCATTTTTACTGACCAGAACATCATTAAACGGGCAAACAAGACGTCCGGCCTCGATTTCAGATTGCGCCATCACGTTATTTGCCAGCGCCACTCCCCGc                                                                                                                                    >  1:587380/1‑140 (MQ=255)        ttCTGCCTGACTGTCATGACAAACCAGATAAAAAGCATTTTTACTGACCAGAACATCATTAAACGGGCAAACAAGACGTCCGGCCTCGATTTCAGATTGCGCCATCACGTTATTTGCCAGCGCCACTCCCCGCCCGTGGa                                                                                                                             >  2:1245645/1‑140 (MQ=255)        ttCTGCCTGACTGTCATGACAAACCAGATAAAAAGCATTTTTACTGACCAGAACATCATTAAACGGGCAAACAAGACGTCCGGCCTCGATTTCAGATTGCGCCATCACGTTATTTGCCAGCGCCACTCCCCGCCCGTGGa                                                                                                                             >  1:536027/1‑140 (MQ=255)        ttCTGCCTGACTGTCATGACAAACCAGATAAAAAGCATTTTTACTGACCAGAACATCATTAAACGGGCAAACAAGACGTCCGGCCTCGATTTCAGATTGCGCCATCACGTTATTTGCCAGCGCCACTCCCCGCCCGTGGa                                                                                                                             >  2:1373314/1‑140 (MQ=255)        ttCTGCCTGACTGTCATGACAAACCAGATAAAAAGCATTTTTACTGACCAGAACATCATTAAACGGGCAAACAAGACGTCCGGCCTCGATTTCAGATTGCGCCATCACGTTATTTGCCAGCGCCACTCCCCGCCCGTGGa                                                                                                                             >  2:204529/1‑140 (MQ=255)        ttCTGCCTGACTGTCATGACAAACCAGATAAAAAGCATTTTTACTGACCAGAACATCATTAAACGGGCAAACAAGACGTCCGGCCTCGATTTCAGATTGCGCCATCACGTTATTTGCCAGCGCCACTCCCCGCCCGTGGa                                                                                                                             >  1:251039/1‑140 (MQ=255)           tGCCTGACTGTCATGACAAACCAGATAAAAAGCATTTTTACTGACAAGAACATCATTAAACGGGCAAACAAGACGTCCGGCCTCGATTTCAGATTGCGCCATCACGTTATTTGCCAGCGCCACTCCCCGCCCGTGGATAg                                                                                                                          <  2:959351/140‑1 (MQ=255)             ccTGACTGTCATGACAAACCAGATAAAAAGCATTTTTACTGACCAGAACATCATTAAACGGGCAAACAAGACGTCCGGCCTCGATTTCAGATTGCGCCATCACGTTATTTGCCAGCGCCACTCCCCGCCCGTGGATAGcc                                                                                                                        <  1:630000/140‑1 (MQ=255)             ccTGACTGTCATGACAAACCAGATAAAAAGCATTTTTACTGACCAGAACATCATTAAACGGGCAAACAAGACGTCCGGCCTCGATTTCAGATTGCGCCATCACGTTATTTGCCAGCGCCACTCCCCGCCCGTGGATAGcc                                                                                                                        <  1:295130/140‑1 (MQ=255)             ccTGACTGTCATGACAAACCAGATAAAAAGCATTTTTACTGACCAGAACATCATTAAACGGGCAAACAAGACGTCCGGCCTCGATTTCAGATTGCGCCATCACGTTATTTGCCAGCGCCACTCCCCGCCCGTGGATAGcc                                                                                                                        >  1:395001/1‑140 (MQ=255)                gACTGTCATGACAAACCAGATAAAAAGCATTTTTACTGACCAGAACATCATTAAACGGGCAAACAAGACGTCCGGCCTCGATTTCAGATTGCGCCATCACGTTATTTGCCAGCGCCACTCCCCGCCCGTGGATAGCCGCt                                                                                                                     <  2:587380/140‑1 (MQ=255)                  cTGTCATGACAAACCAGATAAAAAGCATTTTTACTGACCAGAACATCATTAAACGGGCAAACAAGACGTCCGGCCTCGATTTCAGATTGCGCCATCACGTTATTTGCCAGCGCCACTCCCCGCCCGTGGATAg                                                                                                                          <  1:897739/133‑1 (MQ=255)                  cTGTCATGACAAACCAGATAAAAAGCATTTTTACTGACCAGAACATCATTAAACGGGCAAACAAGACGTCCGGCCTCGATTTCAGATTGCGCCATCACGTTATTTGCCAGCGCCACTCCCCGCCCGTGGATAg                                                                                                                          >  2:897739/1‑133 (MQ=255)                  cTGTCATGACAAACCAGATAAAAAGCATTTTTACTGACCAGAACATCATTAAACGGGCAAACAAGACGTCCGGCCTCGATTTCAGATTGCGCCATCACGTTATTTGCCAGCGCCACTCCCCGCCCGTGGATAGCCGCTTg                                                                                                                   >  1:702626/1‑140 (MQ=255)                  cTGTCATGACAAACCAGATAAAAAGCATTTTTACTGACCAGAACATCATTAAACGGGCAAACAAGACGTCCGGCCTCGATTTCAGATTGCGCCATCACGTTATTTGCCAGCGCCACTCCCCGCCCGTGGATAGCCGCTTg                                                                                                                   >  2:393479/1‑140 (MQ=255)                  cTGTCATGACAAACCAGATAAAAAGCATTTTTACTGACCAGAACATCATTAAACGGGCAAACAAGACGTCCGGCCTCGATTTCAGATTGCGCCATCACGTTATTTGCCAGCGCCACTCCCCGCCCGTGGATAGCCGCTTg                                                                                                                   >  2:1046994/1‑140 (MQ=255)                  cTGTCATGACAAACCAGATAAAAAGCATTTTTACTGACCAGAACATCATTAAACGGGCAAACAAGACGTCCGGCCTCGATTTCAGATTGCGCCATCACGTTATTTGCCAGCGCCACTCCCCGCCCG‑GGATAGCCGCTTgc                                                                                                                  >  2:794226/1‑140 (MQ=255)                     tCATGACAAACCAGATAAAAAGCATTTTTACTGACCAGAACATCATTAAACGGGCAAACAAGACGTCCGGCCTCGATTTCAGATTGCGCCATCACGTTATTTGCCAGCGCCACTCCCCGCCCGTGGATAGCCGCTTgcag                                                                                                                >  1:102155/1‑140 (MQ=255)                       aTGACAAACCAGATAAAAAGCATTTTTACTGACCAGAACATCATTAAACGGGCAAACAAGACGTCCGGCCTCGATTTCAGATTGCGCCATCACGTTATTTGCCAGCGCCACTCCCCGCCCGTGGa                                                                                                                             >  2:479378/1‑125 (MQ=255)                       aTGACAAACCAGATAAAAAGCATTTTTACTGACCAGAACATCATTAAACGGGCAAACAAGACGTCCGGCCTCGATTTCAGATTGCGCCATCACGTTATTTGCCAGCGCCACTCCCCGCCCGTGGa                                                                                                                             <  1:479378/125‑1 (MQ=255)                          aCAAACCAGATAAAAAGCATTTTTACTGACCAGAACATCATTAAACGGGCAAACAAGACGTCCGGCCTCGATTTCAGATTGCGCCATCACGTTATTTGCCAGCGCCACTCCCCGCCCGTGGATAGCCGCTTGCAGCACCa                                                                                                           <  2:702626/140‑1 (MQ=255)                             aaCCAGATAAAAAGCATTTTTACTGACCAGAACATCATTAAACGGGCAAACAAGACGTCCGGCCTCGATTTCAGATTGCGCCATCACGTTATTTGCCAGCGCCACTCCCCGCCCGTGGATAGCCGCTTGCAGCACCATgg                                                                                                        >  2:678715/1‑140 (MQ=255)                               ccAGATAAAAAGCATTTTTGCTGACCAGAACATCATTAAACGGGCAAACAAGACGTCCGGCCTCGATTTCAGATTGCGCCATCACGTTATTTGCCAGCGCCACTCCCCGCCCGTGGATAGCCGCTTgcagca                                                                                                              <  2:587747/132‑1 (MQ=255)                               ccAGATAAAAAGCATTTTTACTGACCAGAACATCATTAAACGGGCAAACAAGACGTCCGGCCTCGATTTCAGATTGCGCCATCACGTTATTTGCCAGCGCCACTCCCCGCCCGTGGATAGCCGCTTgcagca                                                                                                              >  1:587747/1‑132 (MQ=255)                               ccAGATAAAAAGCATTTTTACTGACCAGAACATCATTAAACGGGCAAACAAGACGTCCGGCCTCGATTTCAGATTGCGCCATCACGTTATTTGCCAGCGCCACTCCCCGCCCGTGGATAGCCGCTTGCAGCACCATGgcg                                                                                                      >  1:385410/1‑140 (MQ=255)                                 aGATAAAAAGCATTTTTACTGACCAGAACATCATTAAACGGGCAAACAAGACGTCCGGCCTCGATTTCAGATTGCGCCATCACGTTATTTGCCAGCGCCACTCCCCGCCCGTGGATAGCCGCTTGCAGCACCATGGCGCt                                                                                                    >  1:200525/1‑140 (MQ=255)                                 aGATAAAAAGCATTTTTACTGACCAGAACATCATTAAACGGGCAAACAAGACGTCCGGCCTCGATTTCAGATTGCGCCATCACGTTATTTGCCAGCGCCACTCCCCGCCCGTGGATAGCCGCTTGCAGCACCATGGCGCt                                                                                                    <  1:1046994/140‑1 (MQ=255)                                   aTAAAAAGCATTTTTACTGACCAGAACATCATTAAACGGGCAAACAAGACGTCCGGCCTCGATTTCAGATTGCGCCATCACGTTATTTGCCAGCGCCACTCCCCGCCCGTGGATAGCCGCTTGCAGCACCATGGCGCTAt                                                                                                  >  2:715559/1‑140 (MQ=255)                                         aGCATTTTTACTGACCAGAACATCATTAAACGGGCAAACAAGACGTCCGGCCTCGATTTCAGATTGCGCCATCACGTTATTTGCCAGCGCCACTCCCCGCCCGTGGATAGCCGCTTGCAGCACCATGGCGCTATGGCTaa                                                                                            >  1:1224001/1‑140 (MQ=255)                                              ttttACTGACCAGAACATCATTAAACGGGCAAACAAGACGTCCGGCCTCGATTTCAGATTGCGCCATCACGTTATTTGCCAGCGCCACTCCCCGCCCGTGGATAGCCGCTTGCAGCACCATGGCGCTATGGCTAAAAAtt                                                                                       <  1:322568/140‑1 (MQ=255)                                                  aCTGACCAGAACATCATTAAACGGGCAAACAAGACGTCCGGCCTCGATTTCAGATTGCGCCATCACGTTATTTGCCAGCGCCACTCCCCGCCCGTGGATAGCCGCTTGCAGCACCATGGCGCTATGGCTAAAAATTGGcc                                                                                   <  1:962687/140‑1 (MQ=255)                                                     gACCAGAACATCATTAAACGGGCAAACAAGACGTCCGGCCTCGATTTCAGATTGCGCCATCACGTTATTTGCCAGCGCCACTCCCCGCCCGTGGATAGCCGCTTGCAGCACCATGGCGCTATGGCTAAAAATTGGCCCtt                                                                                >  2:378002/1‑140 (MQ=255)                                                           aaCATCATTAAACGGGCAAACAAGACGTCCGGCCTCGATTTCAGATTGCGCCATCACGTTATTTGCCAGCGCCACTCCCCGCCCGTGGATAGCCGCTTGCAGCACCATGGCGCTATGGCTAAAAATTGGCCCTTGCTGaa                                                                          >  1:1025823/1‑140 (MQ=255)                                                           aaCATCATTAAACGGGCAAACAAGACGTCCGGCCTCGATTTCAGATTGCGCCATCACGTTATTTGCCAGCGCCACTCCCCGCCCGTGGATAGCCGCTTGCAGCACCATGGCGCTATGGCTAAAAATTGGCCCTTGCTGaa                                                                          >  1:201325/1‑140 (MQ=255)                                                             catcatTAAACGGGCAAACAAGACGTCCGGCCTCGATTTCAGATTGCGCCATCACGTTATTTGCCAGCGCCACTCCCCGCCCGTGGATAGCCGCTTGCAGCACCATGGCGCTATGGCTAAAAATTGGCCCTTGCTGAACg                                                                        <  2:536027/140‑1 (MQ=255)                                                                catTAAACGGGCAAACAAGACGTCCGGCCTCGATTTCAGATTGCGCCATCACGTTATTTGCCAGCGCCACTCCCCGCCCGTGGATAGCCGCTTGCAGCACCATGGCGCTATGGCTAAAAATTGGCCCTTGCTGAACGTTg                                                                     >  1:150810/1‑140 (MQ=255)                                                                catTAAACGGGCAAACAAGACGTCCGGCCTCGATTTCAGATTGCGCCATCACGTTATTTGCCAGCGCCACTCCCCGCCCGTGGATAGCCGCTTGCAGCACCATGGCGCTATGGCTAAAAATTGGCCCTTGCTGAACGTTg                                                                     >  1:108491/1‑140 (MQ=255)                                                                catTAAACGGGCAAACAAGACGTCCGGCCTCGATTTCAGATTGCGCCATCACGTTATTTGCCAGCGCCACTCCCCGCCCGTGGATAGCCGCTTGCAGCACCATGGCGCTATGGCTAAAAATTGGCCCTTGCTGAACGTTg                                                                     >  1:121336/1‑140 (MQ=255)                                                                     aaCGGGCAAACAAGACGTCCGGCCTCGATTTCAGATTGCGCCATCACGTTATTTGCCAGCGCCACTCCCCGCCCGTGGATAGCCGCTTGCAGCACCATGGCGCTATGGCTAAAAATTGGCCCTTGCTGAACGTTGATATg                                                                >  1:728231/1‑140 (MQ=255)                                                                       cGGGCAAACAAGACGTCCGGCCTCGATTTCAGATTGCGCCATCACGTTATTTGCCAGCGCCACTCCCCGCCCGTGGATAGCCGCTTGCAGCACCATGGCGCTATGGCTAAAAATTGGCCCTTGCTGAACGTTGATATGAt                                                              <  1:1373314/140‑1 (MQ=255)                                                                       cGGGCAAACAAGACGTCCGGCCTCGATTTCAGATTGCGCCATCACGTTATTTGCCAGCGCCACTCCCCGCCCGTGGATAGCCGCTTGCAGCACCATGGCGCTATGGCTAAAAATTGGCCCTTGCTGAACGTTGATATGAt                                                              >  2:366350/1‑140 (MQ=255)                                                                       cGGGCAAACAAGACGTCCGGCCTCGATTTCAGATTGCGCCATCACGTTATTTGCCAGCGCCACTCCCCGCCCGTGGATAGCCGCTTGCAGCACCATGGCGCTATGGCTAAAAATTGGCCCTTGCTGAACGTTGATATGAt                                                              <  2:1025823/140‑1 (MQ=255)                                                                        gggCAAACAAGACGTCCGGCCTCGATTTCAGATTGCGCCATCACGTTATTTGCCAGCGCCACTCCCCGCCCGTGGATAGCCGCTTGCAGCACCATGGCGCTATGGCTAAAAATTGGCCCTTGCTGAACGTTGATATGAtt                                                             >  1:1303659/1‑140 (MQ=255)                                                                         ggCAAACAAGACGTCCGGCCTCGATTTCAGATTGCGCCATCACGTTATTTGCCAGCGCCACTCCCCGCCCGTGGATAGCCGCTTGCAGCACCATGGCGCTATGGCTAAAAATTGGCCCTTGCTGAACGTTGATATGAttt                                                            >  2:331926/1‑140 (MQ=255)                                                                          gCAAACAAGACGTCCGGCCTCGATTTCAGATTGCGCCATCACGTTATTTGCCAGCGCCGCTCCCCGCCCGTGGATAGCCGCTTGCAGCACCATGGCGCTATGGCTAAAAATTGGCCCTTGCTGAACGTTGATATGATTTa                                                           >  1:1243892/1‑140 (MQ=255)                                                                          gCAAACAAGACGTCCGGCCTCGATTTCAGATTGCGCCATCACGTTATTTGCCAGCGCCACTCCCCGCCCGTGGATAGCCGCTTGCAGCACCATGGCGCTATGGCTAAAAATTGGCCCTTGCTGAACGTTGATATGATTTa                                                           >  1:64562/1‑140 (MQ=255)                                                                          gCAAACAAGACGTCCGGCCTCGATTTCAGATTGCGCCATCACGTTATTTGCCAGCGCCACTCCCCGCCCGTGGATAGCCGCTTGCAGCACCATGGCGCTATGGCTAAAAATTGGCCCTTGCTGAACGTTGATATGATTTa                                                           >  1:669977/1‑140 (MQ=255)                                                                          gCAAACAAGACGTCCGGCCTCGATTTCAGATTGCGCCATCACGTTATTTGCCAGCGCCACTCCCCGCCCGTGGATAGCCGCTTGCAGCACCATGGCGCTATGGCTAAAAATTGGCCCTTGCTGAACGTTGATATGATTTa                                                           >  1:32933/1‑140 (MQ=255)                                                                           cAAACAAGACGTCCGGCCTCGATTTCAGATTGCGCCATCACGTTATTTGCCAGCGCCACTCCCCGCCCGTGGATAGCCGCTTGCAGCACCATGGCGCTATGGCTAAAAATTGGCCCTTGCTGAACGTTGATATGATTTaa                                                          >  2:1120822/1‑140 (MQ=255)                                                                              aCAAGACGTCCGGCCTCGATTTCAGATTGCGCCATCACGTTATTTGCCAGCGCCACTCCCCGCCCGTGGATAGCCGCTTGCAGCACCATGGCGCTATGGCTAAAAATTGGCCCTTGCTGAACGTTGATATGATTTAAccc                                                       <  1:331926/140‑1 (MQ=255)                                                                               cAAGACGTCCGGCCTCGATTTCAGATTGCGCCATCACGTTATTTGCCAGCGCCACTCCCCGCCCGTGGATAGCCGCTTGCAGCACCATGGCGCTATGGCTAAAAATTGGCCCTTGCTGAACGTTGATATGATTTAAcccc                                                      <  2:669977/140‑1 (MQ=255)                                                                                    cGTCCGGCCTCGATTTCAGATTGCGCCATCACGTTATTTGCCAGCGCCACTCCCCGCCCGTGGATAGCCGCTTGCAGCACCATGGCGCTATGGCTAAAAATTGGCCCTTGCTGAACGTTGATATGATTTAACCCCAACTg                                                 >  2:1422081/1‑140 (MQ=255)                                                                                    cGTCCGGCCTCGATTTCAGATTGCGCCATCACGTTATTTGCCAGCGCCACTCCCCGCCCGTGGATAGCCGCTTGCAGCACCATGGCGCTATGGCTAAAAATTGGCCCTTGCTGAACGTTGATATGATTTAACCCCAACTg                                                 <  1:393479/140‑1 (MQ=255)                                                                                     gTCCGGCCTCGATTTCAGATTGCGCCATCACGTTATTTGCCAGCGCCACTCCCCGCCCGTGGATAGCCGCTTGCAGCACCATGGCGCTATGGCTAAAAATTGGCCCTTGCTGAACGTTGATATGATTTAACCCCAACTGt                                                <  1:206231/140‑1 (MQ=255)                                                                                     gTCCGGCCTCGATTTCAGATTGCGCCATCACGTTATTTGCCAGCGCCACTCCCCGCCCGTGGATAGCCGCTTGCAGCACCATGGCGCTATGGCTAAAAATTGGCCCTTGCTGAACGTTGATATGATTTAACCCCAACTGt                                                <  2:592672/140‑1 (MQ=255)                                                                                     gTCCGGCCTCGATTTCAGATTGCGCCATCACGTTATTTGCCAGCGCCACTCCCCGCCCGTGGATAGCCGCTTGCAGCACCATGGCGCTATGGCTAAAAATTGGCCCTTGCTGAACGTTGATATGATTTAACCCCAACTGt                                                <  2:1303659/140‑1 (MQ=255)                                                                                      tCCGGCCTCGATTTCAGATTGCGCCATCACGTTATTTGCCAGCGCCACTCCCCGCCCGTGGATAGCCGCTTGCAGCACCATGGCGCTATGGCTAAAAATTGGCCCTTGCTGAACGTTGATATGATTTAACCCCAACTGTc                                               <  2:385410/140‑1 (MQ=255)                                                                                        cGGCCTCGATTTCAGATTGCGCCATCACGTTATTTGCCAGCGCCACTCCCCGCCCGTGGATAGCCGCTTGCAGCACCATGGCGCTATGGCTAAAAATTGGCCCTTGCTGAACGTTGATATGATTTAACCCCAACTGTCgg                                             >  1:1270587/1‑140 (MQ=255)                                                                                        cGGCCTCGATTTCAGATTGCGCCATCACGTTATTTGCCAGCGCCACTCCCCGCCCGTGGATAGCCGCTTGCAGCACCATGGCGCTATGGCTAAAAATTGGCCCTTGCTGAACGTTGATATGATTTAACCCCAACTGTCgg                                             >  1:26981/1‑140 (MQ=255)                                                                                        cGGCCTCGATTTCAGATTGCGCCATCACGTTATTTGCCAGCGCCACTCCCCGCCCGTGGATAGCCGCTTGCAGCACCATGGCGCTATGGCTAAAAATTGGCCCTTGCTGAACGTTGATATGATTTAACCCCAACTGTCgg                                             >  2:205298/1‑140 (MQ=255)                                                                                         ggCCTCGATTTCAGATTGCGCCATCACGTTATTTGCCAGCGCCACTCCCCGCCCGTGGATAGCCGCTTGCAGCACCATGGCGCTATGGCTAAAAATTGGCCCTTGCTGAACGTTGATATGATTTAACCCCAACTGTCggg                                            <  2:251039/140‑1 (MQ=255)                                                                                          gCCTCGATTTCAGATTGCGCCATCACGTTATTTGCCAGCGCCACTCCCCGCCCGTGGATAGCCGCTTGCAGCACCATGGCGCTATGGCTAAAAATTGGCCCTTGCTGAACGTTGATATGATTTAACCCCAACTGTCGGGt                                           <  2:201325/140‑1 (MQ=255)                                                                                          gCCTCGATTTCAGATTGCGCCATCACGTTATTTGCCAGCGCCACTCCCCGCCCGTGGATAGCCGCTTGCAGCACCATGGCGCTATGGCTAAAAATTGGCCCTTGCTGAACGTTGATATGATTTAACCCCAACTGTCGGGt                                           >  1:240889/1‑140 (MQ=255)                                                                                          gCCTCGATTTCAGATTGCGCCATCACGTTATTTGCCAGCGCCACTCCCCGCCCGTGGATAGCCGCTTGCAGCACCATGGCGCTATGGCTAAAAATTGGCCCTTGCTGAACGTTGATATGATTTAACCCCAACTGTCGGGt                                           <  1:204529/140‑1 (MQ=255)                                                                                              cGATTTCAGATTGCGCCATCACGTTATTTGCCAGCGCCACTCCCCGCCCGTGGATAGCCGCTTGCAGCACCATGGCGCTATGGCTAAAAATTGGCCCTTGCTGAACGTTGATATGATTTAACCCCAACTGTCGGGTatat                                       >  2:148473/1‑140 (MQ=255)                                                                                              cGATTTCAGATTGCGCCATCACGTTATTTGCCAGCGCCACTCCCCGCCCGTGGATAGCCGCTTGCAGCACCATGGCGCTATGGCTAAAAATTGGCCCTTGCTGAACGTTGATATGATTTAACCCCAACTGTCGGGTatat                                       <  2:240889/140‑1 (MQ=255)                                                                                                aTTTCAGATTGCGCCATCACGTTATTTGCCAGCGCCACTCCCCGCCCGTGGATAGCCGCTTGCAGCACCATGGCGCTATGGCTAAAAATTGGCCCTTGCTGAACGTTGATATGATTTAACCCCAACTGTCGGGTATATGt                                     >  1:371113/1‑140 (MQ=255)                                                                                                  ttCAGATTGCGCCATCACGTTATTTGCCAGCGCCACTCCCCGCCCGTGGATAGCCGCTTGCAGCACCATGGCGCTATGGCTAAAAATTGGCCCTTGCTGAACGTTGATATGATTTAACCCCAACTGTCGGGTATATGTCt                                   <  1:1422081/140‑1 (MQ=255)                                                                                                  ttCAGATTGCGCCATCACGTTATTTGCCAGCGCCACTCCCCGCCCGTGGATAGCCGCTTGCAGCACCATGGCGCTATGGCTAAAAATTGGCCCTTGCTGAACGTTGATATGATTTAACCCCAACTGTCGGGTATATGTCt                                   <  2:343930/140‑1 (MQ=255)                                                                                                         tGCGCCATCACGTTATTTGCCAGCGCCACTCCCCGCCCGTGGATAGCCGCTTGCAGCACCATGGCGCTATGGCTAAAAATTGGCCCTTGCTGAACGTTGATATGATTTAACCCCAACTGTCGGGTATATGTCTGCCAGTc                            >  1:14253/1‑140 (MQ=255)                                                                                                          gcgcCATCACGTTATTTGCCAGCGCCACTCCCCGCCCGTGGATAGCCGCTTGCAGCACCATGGCGCTATGGCTAAAAATTGGCCCTTGCTGAACGTTGATATGATTTAACCCCAACTGTCGGGTATATGTCTGCCAGTCa                           <  2:64562/140‑1 (MQ=255)                                                                                                            gcCATCACGTTATTTGCCAGCGCCACTCCCCGCCCGTGGATAGCCGCTTGCAGCACCATGGCGCTATGGCTAAAAATTGGCCCTTGCTGAACGTTGATATGATTTAACCCCAACTGTCGGGTATATGTCTGCCAGTCACg                         <  2:371113/140‑1 (MQ=255)                                                                                                                tCACGTTATTTGCCAGCGCCACTCCCCGCCCGTGGATAGCCGCTTGCAGCACCATGGCGCTATGGCTAAAAATTGGCCCTTGCTGAACGTTGATATGATTTAACCCCAACTGTCGGGTATATGTCTGCCAGTCACGgcgc                     <  2:728231/140‑1 (MQ=255)                                                                                                                 cACGTTATTTGCCAGCGCCACTCCCCGCCCGTGGATAGCCGCTTGCAGCACCATGGCGCTATGGCTAAAAATTGGCCCTTGCTGAACGTTGATATGATTTAACCCCAACTGTCGGGTATATGTCTGCCAGTCACGGcgcg                    >  1:1133185/1‑140 (MQ=255)                                                                                                                      tATTTGCCAGCGCCGCTCCCCGCCCGTGGATAGCCGCTTGCAGCACCATGGCGCTATGGCTAAAAATTGGCCCTTGCTGAACGTTGATATGATTTAACCCCAACTGTCGGGTATATGTCTGCCAGTCACGGCGCGAAGca               <  2:1243892/140‑1 (MQ=255)                                                                                                                      tATTTGCCAGCGCCACTCCCCGCCCGTGGATAGCCGCTTGCAGCACCATGGCGCTATGGCTAAAAATTGGCCCTTGCTGAACGTTGATATGATTTAACCCCAACTGTCGGGTATATGTCTGCCAGTCACGGCGCGAAGca               <  2:102155/140‑1 (MQ=255)                                                                                                                       aTTTGCCAGCGCCACTCCCCGCCCGTGGATAGCCGCTTGCAGCACCATGGCGCTATGGCTAAAAATTGGCCCTTGCTGAACGTTGATATGATTTAACCCCAACTGTCGGGTATATGTCTGCCAGTCACGGCGCGAAGcat              <  1:379142/140‑1 (MQ=255)                                                                                                                        tttGCCAGCGCCACTCCCCGCCCGTGGATAGCCGCTTGCAGCACCATGGCGCTATGGCTAAAAATTGGCCCTTGCTGAACGTTGATATGATTTAACCCCAACTGTCGGGTATATGTCTGCCAGTCACGGCGCGAAGcatc             <  2:32933/140‑1 (MQ=255)                                                                                                                          tGCCAGCGCCACTCCCCGCCCGTGGATAGCCGCTTGCAGCACCATGGCGCTATGGCTAAAAATTGGCCCTTGCTGAACGTTGATATGATTTAACCCCAACTGTCGGGTATATGTCTGCCAGTCACGGCGCGAAGcatcat           >  2:625538/1‑140 (MQ=255)                                                                                                                          tGCCAGCGCCACTCCCCGCCCGTGGATAGCCGCTTGCAGCACCATGGCGCTATGGCTAAAAATTGGCACTTGCTGAACGTTGATATGATTTAACCCCAACTGTCGGGTATATGTCTGCCAGTCACGGCGCGAAGcatcat           >  2:1292619/1‑140 (MQ=255)                                                                                                                           gCCAGCGCCACTCCCCGCCCGTGGATAGCCGCTTGCAGCACCATGGCGCTATGGCTAAAAATTGGCCCTTGCTGAACGTTGATATGATTTAACCCCAACTGTCGGGTATATGTCTGCCAGTCACGGCGCGAAGCATCATg          <  1:715559/140‑1 (MQ=255)                                                                                                                            ccAGCGCCACTCCCCGCCCGTGGATAGCCGCTTGCAGCACCATGGCGCTATGGCTAAAAATTGGCCCTTGCTGAACGTTGATATGATTTAACCCCAACTGTCGGGTATATGTCTGCCAGTCACGGCGCGAAGCATCATGt         >  1:236685/1‑140 (MQ=255)                                                                                                                             cAGCGCCACTCCCCGCCCGTGGATAGCCGCTTGCAGCACCATGGCGCTATGGCTAAAAATTAGCCCTTGCTGAACGTTGATATGATTTAACCCCAACTGTCGGGTATATGTCTGCCAGTCACGGCGCGAAGCATCATGta        <  2:55230/140‑1 (MQ=255)                                                                                                                              aGCGCCACTCCCCGCCCGTGGATAGCCGCTTGCAGCACCATGGCGCTATGGCTAAAAATTGGCCCTTGCTGAACGTTGATATGATTTAACCCCAACTGTCGGGTATATGTCTGCCAGTCACGGcgcg                    <  1:32807/127‑1 (MQ=255)                                                                                                                              aGCGCCACTCCCCGCCCGTGGATAGCCGCTTGCAGCACCATGGCGCTATGGCTAAAAATTGGCCCTTGCTGAACGTTGATATGATTTAACCCCAACTGTCGGGTATATGTCTGCCAGTCACGGcgcg                    >  2:32807/1‑127 (MQ=255)                                                                                                                              aGCGCCACTCCCCGCCCGTGGATAGCCGCTTGCAGCACCATGGCGCTATGGCTAAAAATTGGCCCTTGCTGAACGTTGATATGATTTAACCCCAACTGTCGGGTATATGTCTGCCAGTCACGGCGCGAAGCATCATGtaa       >  2:1325786/1‑140 (MQ=255)                                                                                                                               gcgcCACTCCCCGCCCGTGGATAGCCGCTTGCAGCACCATGGCGCTATGGCTAAAAATTGGCCCTTGCTGAACGTTGATATGATTTAACCCCAACTGTCGGGTATATGTCTGCCAGTCACGGCGCGAAGca               <  1:1089137/131‑1 (MQ=255)                                                                                                                               gcgcCACTCCCCGCCCGTGGATAGCCGCTTGCAGCACCATGGCGCTATGGCTAAAAATTGGCCCTTGCTGAACGTTGATATGATTTAACCCCAACTGTCGGGTATATGTCTGCCAGTCACGGCGCGAAGca               >  2:1089137/1‑131 (MQ=255)                                                                                                                               gcgcCACTCCCCGCCCGTGGATAGCCGCTTGCAGCACCATGGCGCTATGGCTAAAAATTGGCCCTTGCTGAACGTTGATATGATTTAACCCCAACTGTCGGGTATATGTCTGCCAGTCACGGCGCGAAGCATCATGtaat      <  1:547367/140‑1 (MQ=255)                                                                                                                                cgcCACTCCCCGCCCGTGGATAGCCGCTTGCAGCACCATGGCGCTATGGCTAAAAATTGGCCCTTGCTGAACGTTGATATGATTTAACCCCAACTGTCGGGTATATGTCTGCCAGTCACGGCGCGAAGCATCATGtaata     >  1:1318551/1‑140 (MQ=255)                                                                                                                                cgcCACTCCCCGCCCGTGGATAGCCGCTTGCAGCACCATGGCGCTATGGCTAAAAATTGGCCCTTGCTGAACGTTGATATGATTTAACCCCAACTGTCGGGTATATGTCTGCCAGTCACGGCGCGAAGCATCATGtaata     >  1:591163/1‑140 (MQ=255)                                                                                                                                cgcCACTCCCCGCCCGTGGATAGCCGCTTGCAGCACCATGGCGCTATGGCTAAAAATTGGCCCTTGCTGAACGTTGATATGATTTAACCCCAACTGTCGGGTATATGTCTGCCAGTCACGGCGCGAAGCATCATGtaa       >  2:622642/1‑138 (MQ=255)                                                                                                                                cgcCACTCCCCGCCCGTGGATAGCCGCTTGCAGCACCATGGCGCTATGGCTAAAAATTGGCCCTTGCTGAACGTTGATATGATTTAACCCCAACTGTCGGGTATATGTCTGCCAGTCACGGCGCGAAGCATCATGtaa       <  1:622642/138‑1 (MQ=255)                                                                                                                                   cACTCCCCGCCCGTGGATAGCCGCTTGCAGCACCATGGCGCTATGGCTAAAAATTGGCCCTTGCTGAACGTTGATATGATTTAACCCCAACTGTCGGGTATATGTCTGCCAGTCACGGCGCGAAGCATCATGTAATAACg  <  1:1120822/140‑1 (MQ=255)                                                                                                                                   cACTCCCCGCCCGTGGATAGCCGCTTGCAGCACCATGGCGCTATGGCTAAAAATTGGCCCTTGCTGAACGTTGATATGATTTAACCCCAACTGTCGGGTATATGTCTGCCAGTCACGGCGCGAAGCATCATGTAATAACg  <  1:678715/140‑1 (MQ=255)                                                                                                                                   cACTCCCCGCCCGTGGATAGCCGCTTGCAGCACCATGGCGCTATGGCTAAAAATTGGCCCTTGCTGAACGTTGATATGATTTAACCCCAACTGTCGGGTATATGTCTGCCAGTCACGGCGCGAAGCATCATGTAATAACg  <  2:236685/140‑1 (MQ=255)                                                                                                                                   cACTCCCCGCCCGTGGATAGCCGCTTGCAGCACCATGGCGCTATGGCTAAAAATTGGCCCTTGCTGAACGTTGATATGATTTAACCCCAACTGTCGGGTATATGTCTGCCAGTCACGGCGCGAAGCATCATGTAATAACg  <  1:1082138/140‑1 (MQ=255)                                                                                                                                          |                                                                                                                                     TACCCAGTTCTGCCTGACTGTCATGACAAACCAGATAAAAAGCATTTTTACTGACCAGAACATCATTAAACGGGCAAACAAGACGTCCGGCCTCGATTTCAGATTGCGCCATCACGTTATTTGCCAGCGCCACTCCCTGCCCGTGGATAGCCGCTTGCAGCACCATGGCGCTATGGCTAAAAATTGGCCCTTGCTGAACGTTGATATGATTTAACCCCAACTGTCGGGTATATGTCTGCCAGTCACGGCGCGAAGCATCATGTAATAACG  >  NC_000913/2941730‑2941999

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑