Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,971,074	T→C	F219L (TTC→CTC)	ygdQ →	UPF0053 inner membrane protein YgdQ

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,971,074	0	T	C	100.0%	319.0 / NA	85	F219L (TTC→CTC)	ygdQ	UPF0053 inner membrane protein YgdQ
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (42/43);  total (42/43)

ATTGGTGATTTTGTCGAACGCCATCCTTCGGTAAAAATGCTGGCGCTCTCTTTCCTGATTCTGGTGGGCTTTACCCTGATTCTGGAAAGTTTCGACATCCACGTACCGAAAGGTTACATCTACTTCGCGATGTTCTTCTCTATTGCGGTTGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCAAGA  >  NC_000913/2970942‑2971210                                                                                                                                     |                                                                                                                                         aTTGGTGATTTTGTCGAACGCCATCCTTCGGTAAAAATGCTGGCGCTCTCTTTCCTGATTCTGGTGGGCTTTACCCTGATTCTGGAAAGTTTCTACATCCACGTCCCGAAAGGTTACAACTACTTCGCGATGATATtctc                                                                                                                                   <  2:771340/140‑1 (MQ=255)   tGGTGATTTTGTCGAACGCCATCCTTCGGTAAAAATGCTGGCGCTCTCTTTCCTGATTCTGGTGGGCTTTACCCTGATTCTGGAAAGTTTCGACATCCACGTACCGAAAGGTTACATCTACTTCGCGATGCTCTTCTCTa                                                                                                                                 <  2:1349170/140‑1 (MQ=255)            tGTCGAACGCCATCCTTCGGTAAAAATGCTGGCGCTCTCTTTCCTGATTCTGGTGGGCTTTACCCTGATTCTGGAAAGTTTCGACATCCACGTACCGAAAGGTTACATCTACTTCGCGATGCTCTTCTCTAt                                                                                                                                >  2:1309018/1‑132 (MQ=255)            tGTCGAACGCCATCCTTCGGTAAAAATGCTGGCGCTCTCTTTCCTGATTCTGGTGGGCTTTACCCTGATTCTGGAAAGTTTCGACATCCACGTACCGAAAGGTTACATCTACTTCGCGATGCTCTTCTCTAt                                                                                                                                <  1:1309018/132‑1 (MQ=255)            tGTCGAACGCCATCCTTCGGTAAAAATGCTGGCGCTCTCTTTCCTGATTCTGGTGGGCTTTACCCTGATTCTGGAAAGTTTCGACATCCACGTACCGAAAGGTTACATCTACTTCGCGATGCTCTTCTCTATTGCGGTTg                                                                                                                        >  1:168680/1‑140 (MQ=255)              tCGAACGCCATCCTTCGGTAAAAATGCTGGCGCTCTCTTTCCTGATTCTGGTGGGCTTTACCCTGATTCTGGAAAGTTTCGACATCCACGTACCGAAAGGTTACATCTACTTCGCGATGCTCTTCTCTATTGCGGTTGaa                                                                                                                      <  2:1164616/140‑1 (MQ=255)                        tCCTTCGGTAAAAATGCTGGCGCTCTCTTTCCTGATTCTGGTGGGCTTTACCCTGATTCTGGAAAGTTTCGACATCCACGTACCGAAAGGTTACATCTACTTCGCGATGCTCTTCTCTATTGCGGTTGAAAGCCTCAACt                                                                                                            >  1:148264/1‑140 (MQ=255)                              ggTAAAAATGCTGGCGCTCTCTTTCCTGATTCTGGTGGGCTTTACCCTGATTCTGGAAAGTTTCGACATCCACGTACCGAAAGGTTACATCTACTTCGCGATGCTCTTCTCTATTGCGGTTGAAAGCCTCAACTTGATTc                                                                                                      <  2:439576/140‑1 (MQ=255)                              ggTAAAAATGCTGGCGCTCTCTTTCCTGATTCTGGTGGGCTTTACCCTGATTCTGGAAAGTTTCGACATCCACGTACCGAAAGGTTACATCTACTTCGCGATGCTCTTCTCTATTGCGGTTGAAAGCCTCAACTTGATTc                                                                                                      <  1:1103523/140‑1 (MQ=255)                                   aaaTGCTGGCGCTCTCTTTCCTGATTCTGGTGGGCTTTACCCTGATTCTGGAAAGTTTCGACATCCACGTACCGAAAGGTTACATCTACTTCGCGATGCTCTTCTCTATTGCGGTTGAAAGCCTCAACTTGATTCGcaac                                                                                                 >  1:215503/1‑140 (MQ=255)                                          ggCGCTCTCTTTCCTGATTCTGGTGGGCTTTACCCTGATTCTGGAAAGTTTCGACATCCACGTACCGAAAGGTTACATCTACTTCGCGATGCTCTTCTCTATTGCGGTTGAAAGCCTCAACTTGATTCGCAACAAAAAGa                                                                                          >  1:557407/1‑140 (MQ=255)                                           gcgcTCTCTTTCCTGATTCTGGTGGGCTTTACCCTGATTCTGGAAAGTTTCGACATCCACGTACCGAAAGGTTACATCTACTTCGCGATGCTCTTCTCTATTGCGGTTGAAAGCCTCAACTTGATTCGCAACAAAAAGaa                                                                                         <  2:148264/140‑1 (MQ=255)                                             gcTCTCTTTCCTGATTCTGGTGGGCTTTACCCTGATTCTGGAAAGTTTCGACATCCACGTACCGAAAGGTTACATCTACTTCGCGATGCTCTTCTCTATTGCGGTTGAAAGCCTCAACTTGATTCGCAACAAAAAGAATc                                                                                       <  2:466198/140‑1 (MQ=255)                                             gcTCTCTTTCCTGATTCTGGTGGGCTTTACCCTGATTCTGGAAAGTTTCGACATCCACGTACCGAAAGGTTACATCTACTTCGCGATGCTCTTCTCTATTGCGGTTGAAAGCCTCAACTTGATTCGCAACAAAAAGAATc                                                                                       <  2:289697/140‑1 (MQ=255)                                               tctctTTCCTGATTCTGGTGGGCTTTACCCTGATTCTGGAAAGTTTCGACATCCACGTACCGAAAGGTTACATCTACTTCGCGATGCTCTTCTCTATTGCGGTTGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCg                                                                                     <  1:1200770/140‑1 (MQ=255)                                               tctctTTCCTGATTCTGGTGGGCTTTACCCTGATTCTGGAAAGTTTCGACATCCACGTACCGAAAGGTTACATCTACTTCGCGATGCTCTTCTCTATTGCGGTTGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCg                                                                                     <  2:303473/140‑1 (MQ=255)                                               tctctTTCCTGATTCTGGTGGGCTTTACCCTGATTCTGGAAAGTTTCGACATCCACGTACCGAAAGGTTACATCTACTTCGCGATGCTCTTCTCTATTGCGGTTGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCg                                                                                     <  2:168680/140‑1 (MQ=255)                                                 tctTTCCTGATTCTGGTGGGCTTTACCCTGATTCTGGAAAGTTTCGACATCCACGTACCGAAAGGTTACATCTACTTCGCGATGCTCTTCTCTATTGCGGTTGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGct                                                                                   <  2:556047/140‑1 (MQ=255)                                                 tctTTCCTGATTCTGGTGGGCTTTACCCTGATTCTGGAAAGTTTCGACATCCACGTACCGAAAGGTTACATCTACTTCGCGATGCTCTTCTCTATTGCGGTTGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGct                                                                                   <  1:278317/140‑1 (MQ=255)                                                     tCCTGATTCTGGTGGGCTTTACCCTGATTCTGGAAAGTTTCGACATCCACGTACCGAAAGGTTACATCTACTTCGCGATGCTCTTCTCTATTGCGGTTGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGa                                                                               >  2:255362/1‑140 (MQ=255)                                                      ccTGATTCTGGTGGGCTTTACCCTGATTCTGGAAAGTTTCGACATCCACGTACCGAAAGGTTACATCTACTTCGCGATGCTCTTCTCTATTGCGGTTGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGAt                                                                              <  1:223023/140‑1 (MQ=255)                                                        tGATTCTGGTGGGCTTTACCCTGATTCTGGAAAGTTTCGACATCCACGTACCGAAAGGTTACATCTACTTCGCGATGCTCTTCTCTATTGCGGTTGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATAc                                                                            >  2:1072239/1‑140 (MQ=255)                                                          aTTCTGGTGGGCTTTACCCTGATTCTGGAAAGTTTCGACATCCACGTACCGAAAGGTTACATCTACTTCGCGATGCTCTTCTCTATTGCGGTTGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTc                                                                          >  1:400971/1‑140 (MQ=255)                                                          aTTCTGGTGGGCTTTACCCTGATTCTGGAAAGTTTCGACATCCACGTACCGAAAGGTTACATCTACTTCGCGATGCTCTTCTCTATTGCGGTTGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTc                                                                          >  1:1356016/1‑140 (MQ=255)                                                          aTTCTGGTGGGCTTTACCCTGATTCTGGAAAGTTTCGACATCCACGTACCGAAAGGTTACATCTACTTCGCGATGCTCTTCTCTATTGCGGTTGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTc                                                                          >  1:1234703/1‑140 (MQ=255)                                                           ttCTGGTGGGCTTTACCCTGATTCTGGAAAGTTTCGACATCCACGTACCGAAAGGTTACATCTACTTCGCGATGCTCTTCTCTATTGCGGTTGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGct                                                                                   >  1:372983/1‑130 (MQ=255)                                                           ttCTGGTGGGCTTTACCCTGATTCTGGAAAGTTTCGACATCCACGTACCGAAAGGTTACATCTACTTCGCGATGCTCTTCTCTATTGCGGTTGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGct                                                                                   <  2:372983/130‑1 (MQ=255)                                                           ttCTGGTGGGCTTTACCCTGATTCTGGAAAGTTTCGACATCCACGTACCGAAAGGTTACATCTACTTCGCGATGCTCTTCTCTATTGCGGTTGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTcc                                                                         >  1:1196146/1‑140 (MQ=255)                                                           ttCTGGTGGGCTTTACCCTGATTCTGGAAAGTTTCGACATCCACGTACCGAAAGGTTACATCTACTTCGCGATGCTCTTCTCTATTGCGGTTGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTcc                                                                         >  2:200641/1‑140 (MQ=255)                                                           ttCTGGTGGGCTTTACCCTGATTCTGGAAAGTTTCGACATCCACGTACCGAAAGGTTACATCTACTTCGCGATGCTCTTCTCTATTGCGGTTGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTcc                                                                         >  1:1374872/1‑140 (MQ=255)                                                           ttCTGGTGGGCTTTACCCTGATTCTGGAAAGTTTCGACATCCACGTACCGAAAGGTTACATCTACTTCGCGATGCTCTTCTCTATTGCGGTTGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTcc                                                                         <  1:1103828/140‑1 (MQ=255)                                                           ttCTGGTGGGCTTTACCCTGATTCTGGAAAGTTTCGACATCCACGTACCGAAAGGTTACATCTACTTCGCGATGCTCTTCTCTATTGCGGTTGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTcc                                                                         >  2:1039381/1‑140 (MQ=255)                                                             cTGGTGGGCTTTACCCTGATTCTGGAAAGTTTCGACATCCACGTACCGAAAGGTTACATCTACTTCGCGATGCTCTTCTCTATTGCGGTTGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGt                                                                       >  1:1412719/1‑140 (MQ=255)                                                               ggtggGCTTTACCCTGATTCTGGAAAGTTTCGACATCCACGTACCGAAAGGTTACATCTACTTCGCGATGCTCTTCTCTATTGCGGTTGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATa                                                                             >  2:1034225/1‑132 (MQ=255)                                                               ggtggGCTTTACCCTGATTCTGGAAAGTTTCGACATCCACGTACCGAAAGGTTACATCTACTTCGCGATGCTCTTCTCTATTGCGGTTGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATa                                                                             <  1:1034225/132‑1 (MQ=255)                                                               ggtggGCTTTACCCTGATTCTGGAAAGTTTCGACATCCACGTACCGAAAGGTTACATCTACTTCGCGATGCTCTTCTCTATTGCGGTTGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTAc                                                                     >  1:437736/1‑140 (MQ=255)                                                               ggtggGCTTTACCCTGATTCTGGAAAGTTTCGACATCCACGTACCGAAAGGTTACATCTACTTCGCGATGCTCTTCTCTATTGCGGTTGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTAc                                                                     >  1:863602/1‑140 (MQ=255)                                                                gtggGCTTTACCCTGATTCTGGAAAGTTTCGACATCCACGTACCGAAAGGTTACATCTACTTCGCGATGCTCTTCTCTATTGCGGTTGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACg                                                                    >  2:719029/1‑140 (MQ=255)                                                                gtggGCTTTACCCTGATTCTGGAAAGTTTCGACATCCACGTACCGAAAGGTTACATCTACTTCGCGATGCTCTTCTCTATTGCGGTTGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACg                                                                    >  2:628757/1‑140 (MQ=255)                                                                gtggGCTTTACCCTGATTCTGGAAAGTTTCGACATCCACGTACCGAAAGGGTACATCTACTTCGCGATGCTATTCTCTATTGCGGTTGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACg                                                                    <  2:690491/140‑1 (MQ=255)                                                                 tggGCTTTACCCTGATTCTGGAAAGTTTCGACATCCACGTACCGAAAGGTTACATCTACTTCGCGATGCTCTTCTCTATTGCGGTTGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGc                                                                   <  2:1177400/140‑1 (MQ=255)                                                                     cTTTACCCTGATTCTGGAAAGTTTCGACATCCACGTACCGAAAGGTTACATCTACTTCGCGATGCTCTTCTCTATTGCGGTTGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCtctc                                                               <  2:863602/140‑1 (MQ=255)                                                                     cTTTACCCTGATTCTGGAAAGTTTCGACATCCACGTACCGAAAGGTTACATCTACTTCGCGATGCTCTTCTCTATTGCGGTTGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCtctc                                                               <  2:437736/140‑1 (MQ=255)                                                                        tACCCTGATTCTGGAAAGTTTCGACATCCACGTACCGAAAGGTTACATCTACTTCGCGATGCTCTTCTCTATTGCGGTTGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTg                                                            >  2:985683/1‑140 (MQ=255)                                                                          cccTGATTCTGGAAAGTTTCGACATCCACGTACCGAAAGGTTACATCTACTTCGCGATGCTCTTCTCTATTGCGGTTGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCg                                                          >  1:592688/1‑140 (MQ=255)                                                                            cTGATTCTGGAAAGTTTCGACATCCACGTACCGAAAGGTTACATCTACTTCGCGATGCTCTTCTCTATTGCGGTTGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCggg                                                        <  2:1356016/140‑1 (MQ=255)                                                                            cTGATTCTGGAAAGTTTCGACATCCACGTACCGAAAGGTTACATCTACTTCGCGATGCTCTTCTCTATTGCGGTTGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCggg                                                        <  2:400971/140‑1 (MQ=255)                                                                               aTTCTGGAAAGTTTCGACATCCACGTACCGAAAGGTTACATCTACTTCGCGATGCTCTTCTCTATTGCGGTTGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAgg                                                     >  2:341675/1‑140 (MQ=255)                                                                               aTTCTGGAAAGTTTCGACATCCACGTACCGAAAGGTTACATCTACTTCGCGATGCTCTTCTCTATTGCGGTTGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAgg                                                     >  1:1111491/1‑140 (MQ=255)                                                                                   tGGAAAGTTTCGACATCCACGTACCGAAAGGTTACATCTACTTCGCGATGCTCTTCTCTATTGCGGTTGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGa                                                       >  2:1227782/1‑134 (MQ=255)                                                                                   tGGAAAGTTTCGACATCCACGTACCGAAAGGTTACATCTACTTCGCGATGCTCTTCTCTATTGCGGTTGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGa                                                       <  1:1227782/134‑1 (MQ=255)                                                                                    ggAAAGTTTCGACATCCACGTACCGATAGGTTACATCTACTTCGCGATGCTCTTCTCTATTGCGGTTGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTa                                                <  2:1376151/140‑1 (MQ=255)                                                                                      aaaGTTTCGACATCCACGTACCGAAAGGTTACATCTACTTCGCGATGCTCTTCTCTATTGCGGTTGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTact                                              <  1:753559/140‑1 (MQ=255)                                                                                        aGTTTCGACATCCACGTACCGAAAGGTTACATCTACTTCGCGATGCTCTTCTCTATTGCGGTTGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTactca                                            <  1:341675/140‑1 (MQ=255)                                                                                         gTTTCGACATCCACGTACCGAAAGGTTACATCTACTTCGCGATGCTCTTCTCTATTGCGGTTGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTactcac                                           <  2:590655/140‑1 (MQ=255)                                                                                         gTTTCGACATCCACGTACCGAAAGGTTACATCTACTTCGCGATGCTCTTCTCTATTGCGGTTGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTactcac                                           >  1:1364654/1‑140 (MQ=255)                                                                                          tttCGACATCCACGTACCGAAAGGTTACATCTACTTCGCGATGCTCTTCTCTATTGCGGTTGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTactcact                                          >  2:776191/1‑140 (MQ=255)                                                                                             cGACATCCACGTACCGAAAGGTTACATCTACTTCGCGATGCTCTTCTCTATTGCGGTTGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCt                                       <  1:1383979/140‑1 (MQ=255)                                                                                               aCATCCACGTACCGAAAGGTTACATCTACTTCGCGATGCTCTTCTCTATTGCGGTTGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCttt                                     <  1:719029/140‑1 (MQ=255)                                                                                                   ccACGTACCGAAAGGTTACATCTACTTCGCGATGCTCTTCTCTATTGCGGTTGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTAcc                                 >  1:346587/1‑140 (MQ=255)                                                                                                    cACGTACCGAAAGGTTACATCTACTTCGCGATGCTCTTCTCTATTGCGGTTGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGGTACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTAcac                                >  2:835922/1‑138 (MQ=255)                                                                                                      cGTACCGAAAGGTTACATCTAATTCGCGATGCTCTTCTCTATTGCGGTTGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTc                              >  2:357506/1‑140 (MQ=255)                                                                                                       gTACCGAAAGGTTACATCTACTTCGCGATGCTCTTCTCTATTGCGGTTGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTca                             <  1:985683/140‑1 (MQ=255)                                                                                                           cGAAAGGTTACATCTACTTCGCGATGCTCTTCTCTATTGCGGTTGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCttt                                     <  2:732437/128‑1 (MQ=255)                                                                                                           cGAAAGGTTACATCTACTTCGCGATGCTCTTCTCTATTGCGGTTGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCttt                                     >  1:732437/1‑128 (MQ=255)                                                                                                             aaaGGTTACATCTACTTCGCGATGCTCTTCTCTATTGCGGTTGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGttt                       >  2:357236/1‑140 (MQ=255)                                                                                                              aaGGTTACATCTACTTCGCGATGCTCTTCTCTATTGCGGTTGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTa                      <  2:881971/140‑1 (MQ=255)                                                                                                              aaGGTTACATCTACTTCGCGATGCTCTTCTCTATTGCGGTTGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTa                      <  2:592688/140‑1 (MQ=255)                                                                                                                   tACATCTACTTCGCGATGCTCTTCTCTATTGCGGTTGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGAtt                 >  2:695249/1‑140 (MQ=255)                                                                                                                     cATCTACTTCGCGATGCTCTTCTCTATTGCGGTTGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGAtttt               >  2:266908/1‑140 (MQ=255)                                                                                                                     cATCTACTTCGCGATGCTCTTCTCTATTGCGGTTGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGAtttt               >  2:942159/1‑140 (MQ=255)                                                                                                                      aTCTACTTCGCGATGCTCTTCTCTATTGCGGTTGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTc              <  1:357236/140‑1 (MQ=255)                                                                                                                      aTCTACTTCGCGATGCTCTTCTCTATTGCGGTTGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTc              <  2:557407/140‑1 (MQ=255)                                                                                                                        cTACTTCGCGATGCTCTTCTCTATTGCGGTTGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTTTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCt            <  1:1072239/140‑1 (MQ=255)                                                                                                                        cTACTTCGCGATGCTCTTCTCTATTGCGGTTGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCt            <  1:536500/140‑1 (MQ=255)                                                                                                                           cTTCGCGATGCTCTTCTCTATTGCGGTTGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCt         >  2:120710/1‑140 (MQ=255)                                                                                                                            ttCGCGATGCTCTTCTCTATTGCGGTTGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCtt        >  2:939955/1‑140 (MQ=255)                                                                                                                             tCGCGATGCTCTTCTCTATTGCGGTTGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCt            <  2:1234205/135‑1 (MQ=255)                                                                                                                             tCGCGATGCTCTTCTCTATTGCGGTTGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCt            >  1:1234205/1‑135 (MQ=255)                                                                                                                              cgcgATGCTCTTCTCTATTGCGGTTGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTc      >  2:690788/1‑140 (MQ=255)                                                                                                                              cgcgATGCTCTTCTCTATTGCGGTTGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTc      >  2:171268/1‑140 (MQ=255)                                                                                                                               gcgATGCTCTTCTCTATTGCGGTTGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCa     <  2:215503/140‑1 (MQ=255)                                                                                                                                cgATGCTCTTCTCTATTGCGGTTGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCaa    <  2:1364654/140‑1 (MQ=255)                                                                                                                                cgATGCTCTTCTCTATTGCGGTTGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCaa    <  1:835990/140‑1 (MQ=255)                                                                                                                                cgATGCTCTTCTCTATTGCGGTTGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCaa    >  1:382354/1‑140 (MQ=255)                                                                                                                                 gATGCTCTTCTCTATTGCGGTTGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGGTACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACACTCACAGTTTAAGATTTTCATGCTTTCAAg   <  1:835922/140‑1 (MQ=255)                                                                                                                                  aTGCTCTTCTCTATTGCGGTTGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCAAGa  <  2:146342/140‑1 (MQ=255)                                                                                                                                     |                                                                                                                                         ATTGGTGATTTTGTCGAACGCCATCCTTCGGTAAAAATGCTGGCGCTCTCTTTCCTGATTCTGGTGGGCTTTACCCTGATTCTGGAAAGTTTCGACATCCACGTACCGAAAGGTTACATCTACTTCGCGATGTTCTTCTCTATTGCGGTTGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCAAGA  >  NC_000913/2970942‑2971210

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑