Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,017,986	G→A	G643S (GGC→AGC)	ygfK →	putative oxidoreductase, Fe‑S subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,017,986	0	G	A	100.0%	277.3 / NA	77	G643S (GGC→AGC)	ygfK	putative oxidoreductase, Fe‑S subunit
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (49/28);  total (49/28)

TATTCCTGCAGAGTTAATTCAGCACGATATCGATTTTGTTGCCGCTCACGGCGTGAAATTTGAGTATGGCTGCTCACCCGATTTAACCATTGAGCAGTTAAAAAATCAGGGCTTCCACTATGTTCTGATTGCCACCGGCACTGATAAAAATAGCGGTGTGAAACTGGCGGGCGACAACCAAAATGTCTGGAAATCACTCCCCTTCCTGCGTGAATACAACAAGGGTACAGCGCTCAAGCTGGGCAAACATGTGGTCGTTGTCGGGGCGGGTAA  >  NC_000913/3017850‑3018122                                                                                                                                         |                                                                                                                                         tATTCCTGCAGAGTTAATTCAGCACGATATCGATTTTGTTGCCGCTCACGGCGTGAAATTTGAGTATGGCTGCTCACCCGATTTAACCATTGAGCAGTTAAAAAATCAGGGCTTCCACTATGTTCTGATTGCCACCAGCa                                                                                                                                       <  2:897763/140‑1 (MQ=255) tATTCCTGCAGAGTTAATTCAGCACGATATCGATTTTGTTGCCGCTCACGGCGTGAAATTTGAGTATGGCTGCTCACCCGATTTAACCATTGAGCAGTTAAAAAATCAGGGCTTCCACTATGTTCTGATTGCCACCAGCa                                                                                                                                       >  2:1275611/1‑140 (MQ=255)    tCCTGCAGAGTTAATTCAGCACGATATCGATTTTGTTGCCGCTCACGGCGTGAAATTTGAGTATGGCTGCTCACCCGATTTAACCATTGAGCAGTTAAAAAATCAGGGCTTCCACTATGTTCTGATTGCCACCAGCACTg                                                                                                                                    >  1:1326847/1‑140 (MQ=255)        gCAGAGTTAATTCAGCACGATATCGATTTTGTTGCCGCTCACGGCGTGAAATTTGAGTATGGCTGCTCACCCGATTTAACCATTGAGCAGTTAAAAAATCAGGGCTTCCACTATGTTCTGATTGCCACCAGCACTGATaa                                                                                                                                >  2:582387/1‑140 (MQ=255)           gagTTAATTCAGCACGATATCGATTTTGTTGCCGCTCACGGCGTGAAATTTGAGTATGGCTGCTCACCCGATTTAACCATTGAGCAGTTAAAAAATCAGGGCTTCCACTATGTTCTGATTGCCACCAGCACTGAt                                                                                                                                  >  2:1312967/1‑135 (MQ=255)           gagTTAATTCAGCACGATATCGATTTTGTTGCCGCTCACGGCGTGAAATTTGAGTATGGCTGCTCACCCGATTTAACCATTGAGCAGTTAAAAAATCAGGGCTTCCACTATGTTCTGATTGCCACCAGCACTGAt                                                                                                                                  <  1:1312967/135‑1 (MQ=255)           gagTTAATTCAGCACGATATCGATTTTGTTGCCGCTCACGGCGTGAAATTTGAGTATGGCTGCTCACCCGATTTAACCATTGAGCAGTTAAAAAATCAGGGCTTCCACTATGTTCTGATTGCCACCAGCACTGATaaaaa                                                                                                                             <  1:489270/140‑1 (MQ=255)           gagTTAATTCAGCACGATATCGATTTTGTTGCCGCTCACGGCGTGAAATTTGAGTATGGCTGCTCACCCGATTTAACCATTGAGCAGTTAAAAAATCAGGGCTTCCACTATGTTCTGATTGCCACCAGCACTGATaaaaa                                                                                                                             >  2:1079435/1‑140 (MQ=255)              ttAATTCAGCACGATATCGATTTTGTTGCCGCTCACGGCGTGAAATTTGAGTATGGCTGCTCACCCGATTTAACCATTGAGCAGTTAAAAAATCAGGGCTTCCACTATGTTCTGATTGCCACCAGCACTGATAAAAATAg                                                                                                                          >  1:995585/1‑140 (MQ=255)              ttAATTCAGCACGATATCGATTTTGTTGCCGCTCACGGCGTGAAATTTGAGTATGGCTGCTCACCCGATTTAACCATTGAGCAGTTAAAAAATCAGGGCTTCCACTATGTTCTGATTGCCACCAGCACTGATAAAAATAg                                                                                                                          >  1:122622/1‑140 (MQ=255)              ttAATTCAGCACGATATCGATTTTGTTGCCGCTCACGGCGTGAAATTTGAGTATGGCTGCTCACCCGATTTAACCATTGAGCAGTTAAAAAATCAGGGCTTCCACTATGTTCTGATTGCCACCAGCACTGATAAAAATAg                                                                                                                          >  2:1060855/1‑140 (MQ=255)                      gCACGATATCGATTTTGTTGCCGCTCACGGCGTGAAATTTGAGTATGGCTGCTCACCCGATTTAACCATTGAGCAGTTAAAAAATCAGGGCTTCCACTATGTTCTGATTGCCACCAGCACTGATAAAAATAGCGGTGTGa                                                                                                                  >  1:877296/1‑140 (MQ=255)                        aCGATATCGATTTTGTTGCCGCTCACGGCGTGAAATTTGAGTATGGCTGCTCACCCGATTTAACCATTGAGCAGTTAAAAAATCAGGGCTTCCACTATGTTCTGATTGCCACCAGCACTGATAAAAATAGCGGTGTGaaa                                                                                                                >  1:860344/1‑140 (MQ=255)                        aCGATATCGATTTTGTTGCCGCTCACGGCGTGAAATTTGAATATGGCTGCTCACCCGATTTAACCATTGAGCAGTTAAAAAATCAGGGCTTCCACTATGTTCTGATTGCCACCAGCACTGATAAAAATAGCGGTGTGaaa                                                                                                                >  1:1054832/1‑140 (MQ=255)                            tatCGATTTTGTTGCCGCTCACGGCGTGAAATTTGAGTATGGCTGCTCACCCGATTTAACCATTGAGCAGTTAAAAAATCAGGGCTTCCACTATGTTCTGATTGCCACCAGCACTGATAAAAATAGCGGTGTGAAACTgg                                                                                                            >  1:716086/1‑140 (MQ=255)                            tatCGATTTTGTTGCCGCTCACGGCGTGAAATTTGAGTATGGCTGCTCACCCGATTTAACCATTGAGCAGTTAAAAAATCAGGGCTTCCACTATGTTCTGATTGCCACCAGCACTGATAAAAATAGCGGTGTGAAACTgg                                                                                                            >  1:408794/1‑140 (MQ=255)                                           cGCTCACGGCGTGAAATTTGAGTATGGCTGCTCACCCGATTTAACCATTGAGCAGTTAAAAAATCAGGGCTTCCACTATGTTCTGATTGCCACCAGCACTGATAAAAATAGCGGTGTGAAACTGGCGGGCGACAACCaaa                                                                                             <  2:1326847/140‑1 (MQ=255)                                                    cGTGAAATTTGAGTATGGCTGCTCACCCGATTTAACCATTGAGCAGTTAAAAAATCAGGGCTTCCACTATGTTCTGATTGCCACCAGCACTGATAAAAATAGCGGTGTGAAACTGGCGGGCGACAACCAAAATGTCTGGa                                                                                    <  2:716086/140‑1 (MQ=255)                                                                   tGGCTGCTCACCCGATTTAACCATTGAGCAGTTAAAAAATCAGGGCTTCCACTATGTTCTGATTGCCACCAGCACTGATAAAAATAGCGGTGTGAAACTGGCGGGCGACAACCAAAATGTCTGGAAATCACTCCCCTTcc                                                                     >  2:126451/1‑140 (MQ=255)                                                                      ctgctCACCCGATTTAACCATTGAGCAGTTAAAAAATCAGGGCTTCCACTATGTTCTGATTGCCACCAGCACTGATAAAAATAGCGGTGTGAAACTGGCGGGCGACAACCAAAATGTCTGGAAATCACTCCCCTTCCTGc                                                                  <  2:1400970/140‑1 (MQ=255)                                                                         ctCACCCGATTTAACCATTGAGCAGTTAAAAAATCAGGGCTTCCACTATGTTCTGATTGCCACCAGCACTGATAAAAATAGCGGTGTGAAACTGGCGGGCGACAACCAAAATGTCTGGAAATCACTCCCCTTCCTGCGTg                                                               >  1:140921/1‑140 (MQ=255)                                                                         ctCACCCGATTTAACCATTGAGCAGTTAAAAAATCAGGGCTTCCACTATGTTCTGATTGCCACCAGCACTGATAAAAATAGCGGTGTGAAACTGGCGGGCGACAACCAAAATGTCTGGAAATCACTCCCCTTCCTGCGTg                                                               >  1:231683/1‑140 (MQ=255)                                                                         ctCACCCGATTTAACCATTGAGCAGTTAAAAAATCAGGGCTTCCACTATGTTCTGATTGCCACCAGCACTGATAAAAATAGCGGTGTGAAACTGGCGGGCGACAACCAAAATGTCTGGAAATCACTCCCCTTCCTGCGTg                                                               <  1:1325564/140‑1 (MQ=255)                                                                         ctCACCCGATTTAACCATTGAGCAGTTAAAAAATCAGGGCTTCCACTATGTTCTGATTGCCACCAGCACTGATAAAAATAGCGGTGTGAAACTGGCGGGCGACAACCAAAATGTCTGGAAATCACTCCCCTTCCTGCGTg                                                               >  1:878121/1‑140 (MQ=255)                                                                         ctCACCCGATTTAACCATTGAGCAGTTAAAAAATCAGGGCTTCCACTATGTTCTGATTGCCACCAGCACTGATAAAAATAGCGGTGTGAAACTGGCGGGCGACAACCAAAATGTCTGGAAATCACTCCCCTTCCTGCGTg                                                               >  2:215083/1‑140 (MQ=255)                                                                              ccGATTTAACCATTGAGCAGTTAAAAAATCAGGGCTTCCACTATGTTCTGATTGCCACCAGCACTGATAAAAATAGCGGTGTGAAACTGGCGGGCGACAACCAAAATGTCTGGAAATCACTCCCCTTCCTGCGt                                                                <  2:1349212/134‑1 (MQ=255)                                                                              ccGATTTAACCATTGAGCAGTTAAAAAATCAGGGCTTCCACTATGTTCTGATTGCCACCAGCACTGATAAAAATAGCGGTGTGAAACTGGCGGGCGACAACCAAAATGTCTGGAAATCACTCCCCTTCCTGCGt                                                                >  1:1349212/1‑134 (MQ=255)                                                                              ccGATTTAACCATTGAGCAGTTAAAAAATCAGGGCTTCCACTATGTTCTGATTGCCACCAGCACTGATAAAAATAGCGGTGTGAAACTGGCGGGCGACAACCAAAATGTCTGGAAATCACTCCCCTTCCTGCGTGAATac                                                          <  2:1280646/140‑1 (MQ=255)                                                                                gATTTAACCATTGAGCAGTTAAAAAATCAGGGCTTCCACTATGTTCTGATTGCCACCAGCACTGATAAAAATAGCGGTGTGAAACTGGCGGGCGACAACCAAAATGTCTGGAAATCACTCCCCTTCCTGCGTGAATacaa                                                        <  2:878121/140‑1 (MQ=255)                                                                                   ttAACCATTGAGCAGTTAAAAAATCAGGGCTTCCACTATGTTCTGATTGCCACCAGCACTGATAAAAATAGCGGTGTGAAACTGGCGGGCGACAACCAAAATGTCTGGAAATCACTCCCCTTCCTGCGTGAATAcaacaa                                                     >  1:670264/1‑140 (MQ=255)                                                                                   ttAACCATTGAGCAGTTAAAAAATCAGGGCTTCCACTATGTTCTGATTGCCACCAGCACTGATAAAAATAGCGGTGTGAAACTGGCGGGCGACAACCAAAATGTCTGGAAATCACTCCCCTTCCTGCGTGAATAcaacaa                                                     <  2:122622/140‑1 (MQ=255)                                                                                   ttAACCATTGAGCAGTTAAAAAATCAGGGCTTCCACTATGTTCTGATTGCCACCAGCACTGATAAAAATAGCGGTGTGAAACTGGCGGGCGACAACCAAAATGTCTGGAAATCACTCCCCTTCCTGCGTGAATAcaacaa                                                     <  1:126451/140‑1 (MQ=255)                                                                                        cATTGAGCAGTTAAAAAATCAGGGCTTCCACTATGTTCTGATTGCCACCAGCACTGATAAAAATAGCGGTGTGAAACTGGCGGGCGACAACCAAAATGTCTGGAAATCACTCCCCTTCCTGCGTGAATACAACAAGGGTa                                                <  2:995585/140‑1 (MQ=255)                                                                                        cATTGAGCAGTTAAAAAATCAGGGCTTCCACTATGTTCTGATTGCCACCAGCACTGATAAAAATAGCGGTGTGAAACTGGCGGGCGACAACCAAAATGTCTGGAAATCACTCCCCTTCCTGCGTGAATACAACAAGGGTa                                                >  2:475089/1‑140 (MQ=255)                                                                                           tGAGCAGTTAAAAAATCAGGGCTTCCACTATGTTCTGATTGCCACCAGCACTGATAAAAATAGCGGTGTGAAACTGGCGGGCGACAACCAAAATGTCTGGAAATCACTCCCCTTCCTGCGTGAATACAACAAGGGTACAg                                             >  2:678167/1‑140 (MQ=255)                                                                                            gAGCAGTTAAAAAATCAGGGCTTCCACTATGTTCTGATTGCCACCAGCACTGATAAAAATAGCGGTGTGAAACTGGCGGGCGACAACCAAAATGTCTGGAAATCACTCCCCTTCCTGCGTGAATACAACAAGGGTACAgc                                            >  2:601539/1‑140 (MQ=255)                                                                                            gAGCAGTTAAAAAATCAGGGCTTCCACTATGTTCTGATTGCCACCAGCACTGATAAAAATAGCGGTGTGAAACTGGCGGGCGACAACCAAAATGTCTGGAAATCACTCCCCTTCCTGCGTGAATACAACAAGGGTACAgc                                            >  1:1294257/1‑140 (MQ=255)                                                                                            gAGCAGTTAAAAAATCAGGGCTTCCACTATGTTCTGATTGCCACCAGCACTGATAAAAATAGCGGTGTGAAACTGGCGGGCGACAACCAAAATGTCTGGAAATCACTCCCCTTCCTGCGTGAATACAACAAGGGTACAgc                                            >  1:336505/1‑140 (MQ=255)                                                                                            gAGCAGTTAAAAAATCAGGGCTTCCACTATGTTCTGATTGCCACCAGCACTGATAAAAATAGCGGTGTGAAACTGGCGGGCGACAACCAAAATGTCTGGAAATCACTCCCCTTCCTGCGTGAATACAACAAGGGTACAgc                                            >  2:1281537/1‑140 (MQ=255)                                                                                             aGCAGTTAAAAAATCAGGGCTTCCACTATGTTCTGATTGCCACCAGCACTGATAAAAATAGCGGTGTGAAACTGGCGGGCGACAACCAAAATGTCTGGAAATCACTCCCCTTCCTGCGTGAATACAACAAGGGTACAgcg                                           >  2:126085/1‑140 (MQ=255)                                                                                             aGCAGTTAAAAAATCAGGGCTTCCACTATGTTCTGATTGCCACCAGCACTGATAAAAATAGCGGTGTGAAACTGGCGGGCGACAACCAAAATGTCTGGAAATCACTCCCCTTCCTGCGTGAATACAACAAGGGTACAgcg                                           >  1:819985/1‑140 (MQ=255)                                                                                             aGCAGTTAAAAAATCAGGGCTTCCACTATGTTCTGATTGCCACCAGCACTGATAAAAATAGCGGTGTGAAACTGGCGGGCGACAACCAAAATGTCTGGAAATCACTCCCCTTCCTGCGTGAATACAACAAGGGTACAgcg                                           >  1:408076/1‑140 (MQ=255)                                                                                                    aaaaaaTCAGGGCTTCCACTATGTTCTGATTGCCACCAGCACTGATAAAAATAGCGGTGTGAAACTGGCGGGCGACAACCAAAATGTCTGGAAATCACTCCCCTTCCTGCGTGAATACAACAAGGGTACAGCGCTCAAGc                                    >  2:206214/1‑140 (MQ=255)                                                                                                    aaaaaaTCAGGGCTTCCACTATGTTCTGATTGCCACCAGCACTGATAAAAATAGCGGTGTGAAACTGGCGGGCGACAACCAAAATGTCTGGAAATCACTCCCCTTCCTGCGTGAATACAACAAGGGTACAGCGCTCAAGc                                    >  1:522636/1‑140 (MQ=255)                                                                                                    aaaaaaTCAGGGCTTCCACTATGTTCTGATTGCCACCAGCACTGATAAAAATAGCGGTGTGAAACTGGCGGGCGACAACCAAAATGTCTGGAAATCACTCCCCTTCCTGCGTGAATACAACAAGGGTACAGCGCTCAAGc                                    >  1:353815/1‑140 (MQ=255)                                                                                                        aaTCAGGGCTTCCACTATGTTCTGATTGCCACCAGCACTGATAAAAATAGCGGTGTGAAACTGGCGGGCGACAACCAAAATGTCTGGAAATCACTCCCCTTCCTGCGTGAATACAACAAGGGTACAGCGCTCAAGCTggg                                <  1:215083/140‑1 (MQ=255)                                                                                                          tCAGGGCTTCCACTATGTTCTGATTGCCACCAGCACTGATAAAAATAGCGGTGTGAAACTGGCGGGCGACAACCAAAATGTCTGGAAATCACTCCCCTTCCTGCGTGAATACAACAAGGGTACAGCGCTCAAGCTGGGCa                              <  1:601539/140‑1 (MQ=255)                                                                                                            aGGGCTTCCACTATGTTCTGATTGCCACCAGCACTGATAAAAATAGCGGTGTGAAACTGGCGGGCGACAACCAAAATGTCTGGAAATCACTCCCCTTCCTGCGTGAATACAACAAGGGTACAGCGCTCAAGCt                                   >  1:378290/1‑133 (MQ=255)                                                                                                            aGGGCTTCCACTATGTTCTGATTGCCACCAGCACTGATAAAAATAGCGGTGTGAAACTGGCGGGCGACAACCAAAATGTCTGGAAATCACTCCCCTTCCTGCGTGAATACAACAAGGGTACAGCGCTCAAGCt                                   <  2:378290/133‑1 (MQ=255)                                                                                                            aGGGCTTCCACTATGTTCTGATTGCCACCAGCACTGATAAAAATAGCGGTGTGAAACTGGCGGGCGACAACCAAAATGTCTGGAAATCACTCCCCTTCCTGCGTGAATACAACAAGGGTACAGCGCTCAAGCTGGGCaaa                            >  1:288883/1‑140 (MQ=255)                                                                                                            aGGGCTTCCACTATGTTCTGATTGCCACCAGCACTGATAAAAATAGCGGTGTGAAACTGGCGGGCGACAACCAAAATGTCTGGAAATCACTCCCCTTCCTGCGTGAATACAACAAGGGTACAGCGCTCAAGCTGGGCaaa                            >  2:1309460/1‑140 (MQ=255)                                                                                                            aGGGCTTCCACTATGTTCTGATTGCCACCAGCACTGATAAAAATAGCGGTGTGAAACTGGCGGGCGACAACCAAAATGTCTGGAAATCACTCCCCTTCCTGCGTGAATACAACAAGGGTACAGCGCTCAAGCTGGGCaaa                            >  1:256684/1‑140 (MQ=255)                                                                                                            aGGGCTTCCACTATGTTCTGATTGCCACCAGCACTGATAAAAATAGCGGTGTGAAACTGGCGGGCGACAACCAAAATGTCTGGAAATCACTCCCCTTCCTGCGTGAATACAACAAGGGTACAGCGCTCAAGCTGGGCaaa                            >  1:716695/1‑140 (MQ=255)                                                                                                               gCTTCCACTATGTTCTGATTGCCACCAGCACTGATAAAAATAGCGGTGTGAAACTGGCGGGCGACAACCAAAATGTCTGGAAATCACTCCCCTTCCTGCGTGAATACAACAAGGGTACAGCGCTCAAGCTGGGCAAACAt                         <  1:475089/140‑1 (MQ=255)                                                                                                               gCTTCCACTATGTTCTGATTGCCACCAGCACTGATAAAAATAGCGGTGTGAAACTGGCGGGCGACAACCAAAATGTCTGGAAATCACTCCCCTTCCTGCGTGAATACAACAAGGGTACAGCGCTCAAGCTGGGCAAACAt                         <  2:860344/140‑1 (MQ=255)                                                                                                                  tCCACTATGTTCTGATTGCCACCAGCACTGATAAAAATAGCGGTGTGAAACTGGCGGGCGACAACCAAAATGTCTGGAAATCACTCCCCTTCCTGCGTGAATACAACAAGGGTACAGCGCTCAAGCTGGGCAAACAtgtg                      >  2:869864/1‑140 (MQ=255)                                                                                                                    cACTATGTTCTGATTGCCACCAGCACTGATAAAAATAGCGGTGTGAAACTGGCGGGCGACAACCAAAATGTCTGGAAATCACTCCCCTTCCTGCGTGAATACAACAAGGGTACAGCGCTCAAGCTGGGCAAACATGTGGt                    <  2:1103792/140‑1 (MQ=255)                                                                                                                    cACTATGTTCTGATTGCCACCAGCACTGATAAAAATAGCGGTGTGAAACTGGCGGGCGACAACCAAAATGTCTGGAAATCACTCCCCTTCCTGCGTGAATACAACAAGGGTACAGCGCTCAAGCTGGGCAAACATGTGGt                    <  1:126085/140‑1 (MQ=255)                                                                                                                          gTTCTGATTGCCACCAGCACTGATAAAAATAGCGGTGTGAAACTGGCGGGCGACAACCAAAATGTCTGGAAATCACTCCCCTTCCTGCGTGAATACAACAAGGGTACAGCGCTCAAGCTGGGCAAACATGTGGTCGTTGt              <  2:716695/140‑1 (MQ=255)                                                                                                                            tCTGATTGCCACCAGCACTGATAAAAATAGCGGTGTGAAACTGGCGGGCGACAACCAAAATGTCTGGAAATCACTCCCCTTCCTGCGTGAATACAACAAGGGTACAGCGCTCAAGCTGGGCAAACATGTGGTCGTTGTCg            <  1:778089/140‑1 (MQ=255)                                                                                                                            tCTGATTGCCACCAGCACTGATAAAAATAGCGGTGTGAAACTGGCGGGCGACAACCAAAATGTCTGGAAATCACTCCCCTTCCTGCGTGAATACAACAAGGGTACAGCGCTCAAGCTGGGCAAACATGTGGTCGTTGTCg            <  1:1060855/140‑1 (MQ=255)                                                                                                                              tGATTGCCACCAGCACTGATAAAAATAGCGGTGTGAAACTGGCGGGCGACAACCAAAATGTCTGGAAATCACTCCCCTTCCTGCGTGAATACAACAAGGGTACAGCGCTCAAGCTGGGCAAACATGTGGTCGTTGTCggg          <  1:1344411/140‑1 (MQ=255)                                                                                                                              tGATTGCCACCAGCACTGATAAAAATAGCGGTGTGAAACTGGCGGGCGACAACCAAAATGTCTGGAAATCACTCCCCTTCCTGCGTGAATACAACAAGGGTACAGCGCTCAAGCTGGGCAAACATGTGGTCGTTGTCggg          >  1:206655/1‑140 (MQ=255)                                                                                                                              tGATTGCCACCAGCACTGATAAAAATAGCGGTGTGAAACTGGCGGGCGACAACCAAAATGTCTGGAAATCACTCCCCTTCCTGCGTGAATACAACAAGGGTACAGCGCTCAAGCTGGGCAAACATGTGGTCGTTGTCggg          <  1:1281537/140‑1 (MQ=255)                                                                                                                              tGATTGCCACCAGCACTGATAAAAATAGCGGTGTGAAACTGGCGGGCGACAACCAAAATGTCTGGAAATCACTCCCCTTCCTGCGTGAATACAACAAGGGTACAGCGCTCAAGCTGGGCAAACATGTGGTCGTTGTCggg          >  2:509522/1‑140 (MQ=255)                                                                                                                              tGATTGCCACCAGCACTGATAAAAATAGCGGTGTGAAACTGGCGGGCGACAACCAAAATGTCTGGAAATCACTCCCCTTCCTGCGTGAATACAACAAGGGTACAGCGCTCAAGCTGGGCAAACATGTGGTCGTTGTCggg          >  2:666416/1‑140 (MQ=255)                                                                                                                              tGATTGCCACCAGCACTGATAAAAATAGCGGTGTGAAACTGGCGGGCGACAACCAAAATGTCTGGAAATCACTCCCCTTCCTGCGTGAATACAACAAGGGTACAGCGCTCAAGCTGGGCAAACATGTGGTCGTTGTCggg          >  2:668065/1‑140 (MQ=255)                                                                                                                                aTTGCCACCAGCACTGATAAAAATAGCGGTGTGAAACTGGCGGGCGACAACCAAAATGTCTGGAAATCACTCCCCTTCCTGCGTGAATACAACAAGGGTACAGCGCTCAAGCTGGGCAAACATGTGGTCGTTGTCGGGGc        >  2:209551/1‑140 (MQ=255)                                                                                                                                 ttGCCACCAGCACTGATAAAAATAGCGGTGTGAAACTGGCGGGCGACAACCAAAATGTCTGGAAATCACTCCCCTTCCTGCGTGAATACAACAAGGGTACAGCGCTCAAGCTGGGCAAACATGTGGTCGTTGTCGGGGCg       <  2:206655/140‑1 (MQ=255)                                                                                                                                 ttGCCACCAGCACTGATAAAAATAGCGGTGTGAAACTGGCGGGCGACAACCAAAATGTCTGGAAATCACTCCCCTTCCTGCGTGAATACAACAAGGGTACAGCGCTCAAGCTGGGCAAACATGTGGTCGTTGTCGGGGCg       <  1:1079435/140‑1 (MQ=255)                                                                                                                                   gCCACCAGCACTGATAAAAATAGCGGTGTGAAACTGGCGGGCGACAACCAAAATGTCTGGAAATCACTCCCCTTCCTGCGTGAATACAACAAGGGTACAGCGCTCAAGCTGGGCAAACATGTGGTCGTTGTCGGGGCggg     >  1:1085599/1‑140 (MQ=255)                                                                                                                                   gCCACCAGCACTGATAAAAATAGCGGTGTGAAACTGGCGGGCGACAACCAAAATGTCTGGAAATCACTCCCCTTCCTGCGTGAATACAACAAGGGTACAGCGCTCAAGCTGGGCAAACATGTGGTCGTTGTCGGGGCggg     >  1:1157887/1‑140 (MQ=255)                                                                                                                                   gCCACCAGCACTGATAAAAATAGCGGTGTGAAACTGGCGGGCGACAACCAAAATGTCTGGAAATCACTCCCCTTCCTGCGTGAATACAACAAGGGTACAGCGCTCAAGCTGGGCAAACATGTGGTCGTTGTCGGGGCggg     >  1:424201/1‑140 (MQ=255)                                                                                                                                   gCCACCAGCACTGATAAAAATAGCGGTGTGAAACTGGCGGGCGACAACCAAAATGTCTGGAAATCACTCCCCTTCCTGCGTGAATACAACAAGGGTACAGCGCTCAAGCTGGGCAAACATGTGGTCGTTGTCGGGGCggg     >  1:1059681/1‑140 (MQ=255)                                                                                                                                   gCCACCAGCACTGATAAAAATAGCGGTGTGAAACTGGCGGGCGACAACCAAAATGTCTGG‑AATCACTCCCCTTCCTGCGTGAATACAACAAGGGTACAGCGCTCAAGCTGGGCAAACATGTGGTCGTTGTCGGGGCgggg    >  1:1072852/1‑139 (MQ=255)                                                                                                                                    ccaccaGCACTGATAAAAATAGCGGTGTGAAACTGGCGGGCGACAACCAAAATGTCTGGAAATCACTCCCCTTCCTGCGTGAATACAACAAGGGTACAGCGCTCAAGCTGGGCAAACATGTGGTCGTTGTCGGGGCGGGt    <  1:274069/140‑1 (MQ=255)                                                                                                                                    ccaccaGCACTGATAAAAATAGCGGTGTGAAACTGGCGGGCGACAACCAAAATGTCTGGAAATCACTCCCCTTCCTGCGTGAATACAACAAGGGTACAGCGCTCAAGCTGGGCAAACATGTGGTCGTTGTCGGGGCGGGt    <  2:877296/140‑1 (MQ=255)                                                                                                                                     cACCAGCACTGATAAAAATAGCGGTGTGAAACTGGCGGGCGACAACCAAAATGTCTGGAAATCACTCCCCTTCCTGCGTGAATACAACAAGGGTACAGCGCTCAAGCTGGGCAAACATGTGGTCGTTGTCGGGGCGGGTa   >  1:1289646/1‑140 (MQ=255)                                                                                                                                      aCCAGCACTGATAAAAATAGCGGTGTGAAACTGGCGGGCGACAACCAAAATGTCTGGAAATCACTCCCCTTCCTGCGTGAATACAACAAGGGTACAGCGCTCAAGCTGGGCAAACATGTGGTCGTTGTCGGGGCGGGTaa  <  1:850900/140‑1 (MQ=255)                                                                                                                                         |                                                                                                                                         TATTCCTGCAGAGTTAATTCAGCACGATATCGATTTTGTTGCCGCTCACGGCGTGAAATTTGAGTATGGCTGCTCACCCGATTTAACCATTGAGCAGTTAAAAAATCAGGGCTTCCACTATGTTCTGATTGCCACCGGCACTGATAAAAATAGCGGTGTGAAACTGGCGGGCGACAACCAAAATGTCTGGAAATCACTCCCCTTCCTGCGTGAATACAACAAGGGTACAGCGCTCAAGCTGGGCAAACATGTGGTCGTTGTCGGGGCGGGTAA  >  NC_000913/3017850‑3018122

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑