Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	399,946	A→G	S326S (AGT→AGC)	ddlA ←	D‑alanine‑‑D‑alanine ligase A

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	399,946	0	A	G	100.0%	283.9 / NA	76	S326S (AGT→AGC)	ddlA	D‑alanine‑‑D‑alanine ligase A
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (40/36);  total (40/36)

TATTATCGGGCGTTAAAATATTACATTGTGGTTTTCAATGCGTTATCCGCAGCGTGACGCTCCAGCGCCAGTTCAATCAAACGTGTGATCAGATCGGTGTAACCCAGACCGCTGGCTTGCCACAGCTTCGGATACATACTGATATTAGTGAAGCCAGGCAGGGTGTTGATCTCGTTGATCACCACTTCGTTCTCTGGGGTTAAAAACACGTCTACACGCGCCATGCCTGCGCATCCCAACGTTTGATAAGCCTGAACGGCAATCGCCCGGAT  >  NC_000913/399809‑400080                                                                                                                                          |                                                                                                                                       tattatCGGGCGTTAAAATATTACATTGTGGTTTTCAATGCGTTATCCGCAGCGTGACGCTCCAGCGCCAGTTCAATCAAACGTGTGATCAGATCGGTGTAACCCAGACCGCTGGCTTGCCACAGCTTCGGATACATGCt                                                                                                                                      >  2:679032/1‑140 (MQ=255)   ttatCGGGCGTTAAAATATTACATTGTGGTTTTCAATGCGTTATCCGCAGCGTGACGCTCCAGCGCCAGTTCAATCAAACGTGTGATCAGATCGGTGTAACCCAGACCGCTGGCTTGCCACAGCTTCGGATACATGCTGa                                                                                                                                    >  2:247889/1‑140 (MQ=255)   ttatCGGGCGTTAAAATATTACATTGTGGTTTTCAATGCGTTATCCGCAGCGTGACGCTCCAGCGCCAGTTCAATCAAACGTGTGATCAGATCGGTGTAACCCAGACCGCTGGCTTGCCACAGCTTCGGATACATGCTGa                                                                                                                                    >  1:694787/1‑140 (MQ=255)      tCGGGCGTTAAAATATTACATTGTGGTTTTCAATGCGTTATCCGCAGCGTGACGCTCCAGCGCCAGTTCAATCAAACGTGTGATCAGATCGGTGTAACCCAGACCGCTGGCTTGCCACAGCTTCGGATACATGCTGatat                                                                                                                                 >  1:190907/1‑140 (MQ=255)         ggCGTTAAAATATTACATTGTGGTTTTCAATGCGTTATCCGCAGCGTGACGCTCCAGCGCCAGTTCAATCAAACGTGTGATCAGATCGGTGTAACCCAGACCGCTGGCTTGCCACAGCTTCGGATACATGCTGATATTAg                                                                                                                              >  1:531175/1‑140 (MQ=255)         ggCGTTAAAATATTACATTGTGGTTTTCAATGCGTTATCCGCAGCGTGACGCTCCAGCGCCAGTTCAATCAAACGTGTGATCAGATCGGTGTAACCCAGACCGCTGGCTTGCCACAGCTTCGGATACATGCTGATATTAg                                                                                                                              >  2:680866/1‑140 (MQ=255)         ggCGTTAAAATATTACATTGTGGTTTTCAATGCGTTATCCGCAGCGTGACGCTCCAGCGCCAGTTCAATCAAACGTGTGATCAGATCGGTGTAACCCAGACCGCTGGCTTGCCACAGCTTCGGATACATGCTGATATTAg                                                                                                                              >  1:732691/1‑140 (MQ=255)          gCGTTAAAATATTACATTGTGGTTTTCAATGCGTTATCCGCAGCGTGACGCTCCAGCGCCAGTTCAATCAAACGTGTGATCAGATCGGTGTAACCCAGACCGCTGGCTTGCCACAGCTTCGGATACATGCTGATATTAGt                                                                                                                             <  2:1063036/140‑1 (MQ=255)           cGTTAAAATATTACATTGTGGTTTTCAATGCGTTATCCGCAGCGTGACGCTCCAGCGCCAGTTCAATCAAACGTGTGATCAGATCGGTGTAACCCAGACCGCTGGCTTGCCACAGCTTCGGATACATGCTGATATTAGTg                                                                                                                            >  2:211978/1‑140 (MQ=255)           cGTTAAAATATTACATTGTGGTTTTCAATGCGTTATCCGCAGCGTGACGCTCCAGCGCCAGTTCAATCAAACGTGTGATCAGATCGGTGTAACCCAGACCGCTGGCTTGCCACAGCTTCGGATACATGCTGATATTAGTg                                                                                                                            <  2:1416031/140‑1 (MQ=255)             ttAAAATATTACATTGTGGTTTTCAATGCGTTATCCGCAGCGTGACGCTCCAGCGCCAGTTCAATCAAACGTGTGATCAGATCGGTGTAACCCAGACCGCTGGCTTGCCACAGCTTCGGATACATGCTGATATTAg                                                                                                                              >  2:180740/1‑136 (MQ=255)             ttAAAATATTACATTGTGGTTTTCAATGCGTTATCCGCAGCGTGACGCTCCAGCGCCAGTTCAATCAAACGTGTGATCAGATCGGTGTAACCCAGACCGCTGGCTTGCCACAGCTTCGGATACATGCTGATATTAg                                                                                                                              <  1:180740/136‑1 (MQ=255)                   tatTACATTGTGGTTTTCAATGCGTTATCCGCAGCGTGACGCTCCAGCGCCAGTTCAATCAAACGTGTGATCAGATCGGTGTAACCCAGACCGCTGGCTTGCCACAGCTTCGGATACATGCTGATATTAGTGAAGCcagg                                                                                                                    <  1:940565/140‑1 (MQ=255)                    atTACATTGTGGTTTTCAATGCGTTATCCGCAGCGTGACGCTCCAGCGCCAGTTCAATCAAACGTGTGATCAGATCGGTGTAACCCAGACCGCTGGCTTGCCACAGCTTCGGATACATGCTGATATTAGTGAAGCcaggc                                                                                                                   >  2:664328/1‑140 (MQ=255)                     ttACATTGTGGTTTTCAATGCGTTATCCGCAGCGTGACGCTCCAGCGCCAGTTCAATCAAACGTGTGATCAGATCGGTGTAACCCAGACCGCTGGCTTGCCACAGCTTCGGATACATGCTGATATTAGTGAAGCcaggca                                                                                                                  <  2:732691/140‑1 (MQ=255)                     ttACATTGTGGTTTTCAATGCGTTATCCGCAGCGTGACGCTCCAGCGCCAGTTCAATCAAACGTGTGATCAGATCGGTGTAACCCAGACCGCTGGCTTGCCACAGCTTCGGATACATGCTGATATTAGTGAAGCcaggca                                                                                                                  <  1:569125/140‑1 (MQ=255)                     ttACATTGTGGTTTTCAATGCGTTATCCGCAGCGTGACGCTCCAGCGCCAGTTCAATCAAACGTGTGATCAGATCGGTGTAACCCAGACCGCTGGCTTGCCACAGCTTCGGATACATGCTGATATTAGTGAAGCcaggca                                                                                                                  <  1:774474/140‑1 (MQ=255)                      tACATTGTGGTTTTCAATGCGTTATCCGCAGCGTGACGCTCCAGCGCCAGTTCAATCAAACGTGTGATCAGATCGGTGTAACCCAGACCGCTGGCTTGCCACAGCTTCGGATACATGCTGATATTAGTGAAGCcaggcag                                                                                                                 >  2:1179362/1‑140 (MQ=255)                       aCATTGTGGTTTTCAATGCGGTATCCGCAGCGTGCCGCTCCAGCGCCAGTTCCATCAAACGTGTGATCAGATCGGTGTAGCCCAGACCGCTGGCTTGCCACAGCTTCGGATACATGCTGGTATTAGGGGGGCcaggcagg                                                                                                                >  2:940565/1‑140 (MQ=255)                        cATTGTGGTTTTCAATGCGTTATCCGCAGCGTGACGCTCCAGCGCCAGTTCAATCAAACGTGTGATCAGATCGGTGTAACCCAGACCGCTGGCTTGCCACAGCTTCGGATACATGCTGATATTAGTGAAGCCAGGCAggg                                                                                                               >  2:799724/1‑140 (MQ=255)                         aTTGTGGTTTTCAATGCGTTATCCGCAGCGTGACGCTCCAGCGCCAGTTCAATCAAACGTGTGATCAGATCGGTGTAACCCAGACCGCTGGCTTGCCACAGCTTCGGATACATGCTGATATTAGTGAAGCcaggcaggtt                                                                                                              <  2:919890/140‑3 (MQ=255)                           tgtgGTTTTCAATGCGTTATCCGCAGCGTGACGCTCCAGCGCCAGTTCAATCAAACGTGTGATCAGATCGGTGTAACCCAGACCGCTGGCTTGCCACAGCTTCGGATACATGCTGATATTAGTGAAGCCAGGCAGGgtgt                                                                                                            >  2:824402/1‑140 (MQ=255)                            gtgGTTTTCAATGCGTTATCCGCAGCGTGACGCTCCAGCGCCAGTTCAATCAAACGTGTGATCAGATCGGTGTAACCCAGACCGCTGGCTTGCCACAGCTTCGGATACATGCTGATATTAGTGAAGCCAGGCATGGTGtt                                                                                                           >  2:1111328/1‑140 (MQ=255)                            gtgGTTTTCAATGCGTTATCCGCAGCGTGACGCTCCAGCGCCAGTTCAATCAAACGTGTGATCAGATCGGTGTAACCCAGACCGCTGGCTTGCCACAGCTTCGGATACATGCTGATATTAGTGAAGCCAGGCAGGGTGtt                                                                                                           >  1:436100/1‑140 (MQ=255)                             tgGTTTTCAATGCGTTATCCGCAGCGTGACGCTCCAGCGCCAGTTCAATCAAACGTGTGATCAGATCGGTGTAACCCAGACCGCTGGCTTGCCACAGCTTCGGATACATGCTGATATTAGTGAAGc                                                                                                                        <  1:101080/126‑1 (MQ=255)                             tgGTTTTCAATGCGTTATCCGCAGCGTGACGCTCCAGCGCCAGTTCAATCAAACGTGTGATCAGATCGGTGTAACCCAGACCGCTGGCTTGCCACAGCTTCGGATACATGCTGATATTAGTGAAGc                                                                                                                        >  2:101080/1‑126 (MQ=255)                             tgGTTTTCAATGCGTTATCCGCAGCGTGACGCTCCAGCGCCAGTTCAATCAAACGTGTGATCAGATCGGTGTAACCCAGACCGCTGGCTTGCCACAGCTTCGGATACATGCTGATATTAGTGAAGCCAGGCAGGGTGTTg                                                                                                          >  2:829598/1‑140 (MQ=255)                             tgGTTTTCAATGCGTTATCCGCAGCGTGACGCTCCAGCGCCAGTTCAATCAAACGTGTGATCAGATCGGTGTAACCCAGACCGCTGGCTTGCCACAGCTTCGGATACATGCTGATATTAGTGAAGCCAGGCAGGGTGTTg                                                                                                          >  1:528918/1‑140 (MQ=255)                             tgGTTTTCAATGCGTTATCCGCAGCGTGACGCTCCAGCGCCAGTTCAATCAAACGTGTGATCAGATCGGTGTAACCCAGACCGCTGGCTTGCCACAGCTTCGGATACATGCTGATATTAGTGAAGCCAGGCAGGGTGTTg                                                                                                          >  1:820201/1‑140 (MQ=255)                             tgGTTTTCAATGCGTTATCCGCAGCGTGACGCTCCAGCGCCAGTTCAATCAAACGTGTGATCAGATCGGTGTAACCCAGACCGCTGGCTTGCCACAGCTTCGGATACATGCTGATATTAGTGAAGCCAGGCAGGGTGTTg                                                                                                          <  1:1179362/140‑1 (MQ=255)                                 tttCAATGCGTTATCCGCAGCGTGACGCTCCAGCGCCAGTTCAATCAAACGTGTGATCAGATCGGTGTAACCCAGACCGCTGGCTTGCCACAGCTTCGGATACATGCTGATATTAGTGAAGCCAGGCAGGGTGTTGAtct                                                                                                      <  1:247889/140‑1 (MQ=255)                                   tCAATGCGTTATCCGCAGCGTGACGCTCCAGCGCCAGTTCAATCAAACGTGTGATCAGATCGGTGTAACCCAGACCGCTGGCTTGCCACAGCTTCGGATACATGCTGATATTAGTGAAGCCAGGCAGGGTGTTGATCTCg                                                                                                    <  1:1015910/140‑1 (MQ=255)                                        gCGTTATCCGCAGCGTGACGCTCCAGCGCCAGTTCAATCAAACGTGTGATCAGATCGGTGTAACCCAGACCGCTGGCTTGCCACAGCTTCGGATACATGCTGATATTAGTGAAGCCAGGCAGGGTGTTGATCTCGTTGAt                                                                                               <  2:694787/140‑1 (MQ=255)                                          gTTATCCGCAGCGTGACGCTCCAGCGCCAGTTCAATCAAACGTGTGATCAGATCGGTGTAACCCAGACCGCTGGCTTGCCACAGCTTCGGATACATGCTGATATTAGTGAAGCCAGGCAGGGTGTTGATCTCGTTGATca                                                                                             <  1:1072202/140‑1 (MQ=255)                                                 gCAGCGTGACGCTCCAGCGCCAGTTCAATCAAACGTGTGATCAGATCGGTGTAACCCAGACCGCTGGCTTGCCACAGCTTCGGATACATGCTGATATTAGTGAAGCCAGGCAGGGTGTTGATCTCGTTGATCACCACTTc                                                                                      <  1:824402/140‑1 (MQ=255)                                                     cGTGACGCTCCAGCGCCAGTTCAATCAAACGTGTGATCAGATCGGTGTAACCCAGACCGCTGGCTTGCCACAGCTTCGGATACATGCTGATATTAGTGAAGCCAGGCAGGGTGTTGATCTCGTTGATca                                                                                             >  2:474174/1‑129 (MQ=255)                                                     cGTGACGCTCCAGCGCCAGTTCAATCAAACGTGTGATCAGATCGGTGTAACCCAGACCGCTGGCTTGCCACAGCTTCGGATACATGCTGATATTAGTGAAGCCAGGCAGGGTGTTGATCTCGTTGATca                                                                                             <  1:474174/129‑1 (MQ=255)                                                         aCGCTCCAGCGCCAGTTCAATCAAACGTGTGATCAGATCGGTGTAACCCAGACCGCTGGCTTGCCACAGCTTCGGATACATGCTGATATTAGTGAAGCCAGGCATGGTGTTGATCTCGTTGATCACCACTTCGTTCTCTg                                                                              <  1:1111328/140‑1 (MQ=255)                                                           gCTCCAGCGCCAGTTCAATCAAACGTGTGATCAGATCGGTGTAACCCAGACCGCTGGCTTGCCACAGCTTCGGATACATGCTGATATTAGTGAAGCCAGGCAGGGTGTTGATCTCGTTGATCACCACTTCGTTCTCTggg                                                                            <  2:820201/140‑1 (MQ=255)                                                            cTCCAGCGCCAGTTCAATCAAACGTGTGATCAGATCGGTGTAACCCAGACCGCTGGCTTGCCACAGCTTCGGATACATGCTGATATTAGTGAAGCCAGGCAGGGTGTTGATCTCGTTGATCACCACTTCGTTCTCTgggg                                                                           <  1:664328/140‑1 (MQ=255)                                                              ccAGCGCCAGTTCAATCAAACGTGTGATCAGATCGGTGTAACCCAGACCGCTGGCTTGCCACAGCTTCGGATACATGCTGATATTAGTGAAGCCAGGCAGGGTGTTGATCTCGTTGATCACCACTTCGTTCTCTGGGGtt                                                                         <  1:799724/140‑1 (MQ=255)                                                                   gcCAGTTCAATCAAACGTGTGATCAGATCGGTGTAACCCAGACCGCTGGCTTGCCACAGCTTCGGATACATGCTGATATTAGTGAAGCCAGGCAGGGTGTTGATCTCGTTGATCACCACTTCGTTCTCTGGGGTTaaaaa                                                                    <  1:680866/140‑1 (MQ=255)                                                                             tcaaACGTGTGATCAGATCGGTGTAACCCAGACCGCTGGCTTGCCACAGCTTCGGATACATGCTGATATTAGTGAAGCCAGGCAGGGTGTTGATCTCGTTGATCACCACTTCGTTCTCTGGGGTTAAAAACACGTCTaca                                                          <  2:772537/140‑1 (MQ=255)                                                                                  cGTGTGATCAGATCGGTGTAACCCAGACCGCTGGCTTGCCACAGCTTCGGATACATGCTGATATTAGTGAAGCCAGGCAGGGTGTTGATCTCGTTGATCACCACTTCGTTCTCTGGGGTTAAAAACACGTCTACACgcgc                                                     <  2:531175/140‑1 (MQ=255)                                                                                    tgtgATCAGATCGGTGTAACCCAGACCGCTGGCTTGCCACAGCTTCGGATACATGCTGATATTAGTGAAGCCAGGCAGGGTGTTGATCTCGTTGATCACCACTTCGTTCTCTGGGGTTAAAAACACGTCTACACGCGCCa                                                   >  2:860720/1‑140 (MQ=255)                                                                                         tCAGATCGGTGTAACCCAGACCGCTGGCTTGCCACAGCTTCGGATACATGCTGATATTAGTGAAGCCAGGCAGGGTGTTGATCTCGTTGATCACCACTTCGTTCTCTGGGGTTAAAAACACGTCTACACGCGCCATGCCt                                              >  2:757704/1‑140 (MQ=255)                                                                                           aGATCGGTGTAACCCAGACCGCTGGCTTGCCACAGCTTCGGATACATGCTGATATTAGTGAAGCCAGGCAGGGTGTTGATCTCGTTGATCACCACTTCGTTCTCTGGGGTTAAAAACACGTCTACACGCGCCATGCCTgc                                            >  1:127860/1‑140 (MQ=255)                                                                                            gATCGGTGTAACCCAGACCGCTGGCTTGCCACAGCTTCGGATACATGCTGATATTAGTGAAGCCAGGCAGGGTGTTGATCTCGTTGATCACCACTTCGTTCTCTGGGGTTAAAAACACGTCTACACGCGCCATGCCTgcg                                           <  1:860720/140‑1 (MQ=255)                                                                                            gATCGGTGTAACCCAGACCGCTGGCTTGCCACAGCTTCGGATACATGCTGATATTAGTGAAGCCAGGCAGGGTGTTGATCTCGTTGATCACCACTTCGTTCTCTGGGGTTAAAAACACGTCTACACGCGCCATGCCTgcg                                           <  1:829598/140‑1 (MQ=255)                                                                                            gATCGGTGTAACCCAGACCGCTGGCTTGCCACAGCTTCGGATACATGCTGATATTAGTGAAGCCAGGCAGGGTGTTGATCTCGTTGATCACCACTTCGTTCTCTGGGGTTAAAAACACGTCTACACGCGCCATGCCTgcg                                           <  2:1120193/140‑1 (MQ=255)                                                                                             aTCGGTGTAACCCAGACCGCTGGCTTGCCACAGCTTCGGATACATGCTGATATTAGTGAAGCCAGGCAGGGTGTTGATCTCGTTGATCACCACTTCGTTCTCTGGGGTTAAAAACACGTCTACACGCGCCATGCCTgcgc                                          >  2:1303385/1‑140 (MQ=255)                                                                                             aTCGGTGTAACCCAGACCGCTGGCTTGCCACAGCTTCGGATACATGCTGATATTAGTGAAGCCAGGCAGGGTGTTGATCTCGTTGATCACCACTTCGTTCTCTGGGGTTAAAAACACGTCTACACGCGCCATGCCTgcgc                                          >  1:310967/1‑140 (MQ=255)                                                                                              tCGGTGTAACCCAGACCGCTGGCTTGCAACAGCTTCGGATACATGCTGATATTAGTGAAGCCAGGCAGGGTGTTGATCTCGTTGATCACCACTTCGTTCTCTGGGGCTAAAAACACGTCTACACTCGCCATCCCTGCGCa                                         >  2:1172613/1‑140 (MQ=255)                                                                                                  tgtAACCCAGACCGCTGGCTTGCCACAGCTTCGGATACATGCTGATATTAGTGAAGCCAGGCAGGGTGTTGATCTCGTTGATCACCACTTCGTTCTCTGGGGTTAAAAACACGTCTACACGCGCCATGCCTGCGCATccc                                     >  2:529606/1‑140 (MQ=255)                                                                                                  tgtAACCCAGACCGCTGGCTTGCCACAGCTTCGGATACATGCTGATATTAGTGAAGCCAGGCAGGGTGTTGATCTCGTTGATCACCACTTCGTTCTCTGGGGTTAAAAACACGTCTACACGCGCCATGCCTGCGCATccc                                     <  1:928831/140‑1 (MQ=255)                                                                                                  tgtAACCCAGACCGCTGGCTTGCCACAGCTTCGGATACATGCTGATATTAGTGAAGCCAGGCAGGGTGTTGATCTCGTTGATCACCACTTCGTTCTCTGGGGTTAAAAACACGTCTACACGCGCCATGCCTGCGCATccc                                     <  1:1045517/140‑1 (MQ=255)                                                                                                  tgtAACCCAGACCGCTGGCTTGCCACAGCTTCGGATACATGCTGATATTAGTGAAGCCAGGCAGGGTGTTGATCTCGTTGATCACCACTTCGTTCTCTGGGGTTAAAAACACGTCTACACGCGCCATGCCTGCGCATccc                                     <  2:310967/140‑1 (MQ=255)                                                                                                      aCCCAGACCGCTGGCTTGCCACAGCTTCGGATACATGCTGATATTAGTGAAGCCAGGCAGGGTGTTGATCTCGTTGATCACCACTTCGTTCTCTGGGGTTAAAAACACGTCTACACGCGCCATGCCTGCGCATCCCAACg                                 <  1:211978/140‑1 (MQ=255)                                                                                                      aCCCAGACCGCTGGCTTGCCACAGCTTCGGATACATGCTGATATTAGTGAAGCCAGGCAGGGTGTTGATCTCGTTGATCACCACTTCGTTCTCTGGGGTTAAAAACACGTCTACACGCGCCATGCCTGCGCATCCCAACg                                 <  1:757704/140‑1 (MQ=255)                                                                                                      aCCCAGACCGCTGGCTTGCCACAGCTTCGGATACATGCTGATATTAGTGAAGCCAGGCAGGGTGTTGATCTCGTTGATCACCACTTCGTTCTCTGGGGTTAAAAACACGTCTACACGCGCCATGCCTGCGCATCCCAACg                                 <  2:216584/140‑1 (MQ=255)                                                                                                       cccAGACCGCTGGCTTGCCACAGCTTCGGATACATGCTGATATTAGTGAAGCCAGGCAGGGTGTTGATCTCGTTGATCACCACTTCGTTCTCTGGGGTTAAAAACACGTCTACACGCGCCATGCCTGCGCATCCCAACGt                                >  1:261890/1‑140 (MQ=255)                                                                                                       cccAGACCGCTGGCTTGCCACAGCTTCGGATACATGCTGATATTAGTGAAGCCAGGCAGGGTGTTGATCTCGTTGATCACCACTTCGTTCTCTGGGGTTAAAAACAAGTCTACACGCGCCATGCCTGCGCATCCCAACGt                                >  1:273271/1‑140 (MQ=255)                                                                                                           gACCGCTGGCTTGCCACAGCTTCGGATACATGCTGATATTAGTGAAGCCAGGCAGGGTGTTGATCTCGTTGATCACCACTTCGTTCTCTGGGGTTAAAAACACGTCTACACGCGCCATGCCTGCGCATCCCAACGTTTGa                            <  2:436100/140‑1 (MQ=255)                                                                                                            actgCTGGCTTGCCACAGCTTCGGATACATGCTGATATTAGTGAAGCCAGGCAGGGTGTTGATCTCGTTGATCACCACTTCGTTCTCTGGGGTTAAAAACACGTCTACACGCGCCATGCCTGCGCATCCCAACg                                 <  2:938916/131‑1 (MQ=255)                                                                                                            actgCTGGCTTGCCACAGCTTCGGATACATGCTGATATTAGTGAAGCCAGGCAGGGTGTTGATCTCGTTGATCACCACTTCGTTCTCTGGGGTTAAAAACACGTCTACACGCGCCATGCCTGCGCATCCCAACg                                 >  1:938916/4‑134 (MQ=255)                                                                                                            aCCGCTGGCTTGCCACAGCTTCGGATACATGCTGATATTAGTGAAGCCAGGCAGGGTGTTGATCTCGTTGATCACCACTTCGTTCTCTGGGGTTAAAAACACGTCTACACGCGCCATGCCTGCGCATCCCAACGTTTGAt                           >  2:474051/1‑140 (MQ=255)                                                                                                            aCCGCTGGCTTGCCACAGCTTCGGATACATGCTGATATTAGTGAAGCCAGGCAGGGTGTTGATCTCGTTGATCACCACTTCGTTCTCTGGGGTTAAAAACACGTCTACACGCGCCATGCCTGCGCATCCCAACGTTTGAt                           >  1:115851/1‑140 (MQ=255)                                                                                                            aCCGCTGGCTTGCCACAGCTTCGGATACATGCTGATATTAGTGAAGCCAGGCAGGGTGTTGATCTCGTTGATCACCACTTCGTTCTCTGGGGTTAAAAACACGTCTACACGCGCCATGCCTGCGCATCCCAACGTTTGAt                           >  2:1151179/1‑140 (MQ=255)                                                                                                            aCCGCTGGCTTGCCACAGCTTCGGATACATGCTGATATTAGTGAAGCCAGGCAGGGTGTTGATCTCGTTGATCACCACTTCGTTCTCTGGGGTTAAAAACACGTCTACACGCGCCATGCCTGCGCATCCCAACGTTTGAt                           >  2:1068487/1‑140 (MQ=255)                                                                                                            aCCGCTGGCTTGCCACAGCTTCGGATACATGCTGATATTAGTGAAGCCAGGCAGGGTGTTGATCTCGTTGATCACCACTTCGTTCTCTGGGGTTAAAAACACGTCTACACGCGCCATGCCTGCGCATCCCAACGTTTGAt                           >  2:770037/1‑140 (MQ=255)                                                                                                            aCCGCTGGCTTGCCACAGCTTCGGATACATGCTGATATTAGTGAAGCCAGGCAGGGTGTTGATCTCGTTGATCACCACTTCGTTCTCTGGGGTTAAAAACACGTCTACACGCGCCATGCCTGCGCATCCCAACGTTTGAt                           >  1:1078987/1‑140 (MQ=255)                                                                                                                 tGGCTTGCCACAGCTTCGGATACATGCTGATATTAGTGAAGCCAGGCAGGGTGTTGATCTCGTTGATCACCACTTCGTTCTCTGGGGTTAAAAACACGTCTACACGCGCCATGCCTGCGCATCCCAACGTTTGATAAGcc                      >  1:1035530/1‑140 (MQ=255)                                                                                                                 tGGCTTGCCACAGCTTCGGATACATGCTGATATTAGTGAAGCCAGGCAGGGTGTTGATCTCGTTGATCACCACTTCGTTCTCTGGGGTTAAAAACACGTCTACACGCGCCATGCCTGCGCATCCCAACGTTTGATAAGcc                      >  2:1420852/1‑140 (MQ=255)                                                                                                                 tGGCTTGCCACAGCTTCGGATACATGCTGATATTAGTGAAGCCAGGCAGGGTGTTGATCTCGTTGATAACCACTTCGTTCTCTGGGGTTAAAAACACGTCTACACGCGCCATGCCTGCGCATCCCAACGTTTGATAAGcc                      >  1:1247197/1‑140 (MQ=255)                                                                                                                        ccACAGCTTCGGATACATGCTGATATTAGTGAAGCCAGGCAGGGTGTTGATCTCGTTGATCACCACTTCGTTCTCTGGGGTTAAAAACACGTCTACACGCGCCATGCCTGCGCATCCCAACGTTTGATAAGCCTGAACgg               >  1:265042/1‑140 (MQ=255)                                                                                                                            aGCTTCGGATACATGCTGATATTAGTGAAGCCAGGCAGGGTGTTGATCTCGTTGATCACCACTTCGTTCTCTGGGGTTAAAAACACGTCTACACGCGCCATGCCTGCGCATCCCAACGTTTGATAAGCCTGAACGGCAAt           <  1:1151179/140‑1 (MQ=255)                                                                                                                               ttCGGATACATGCTGATATTAGTGAAGCCAGGCAGGGTGTTGATCTCGTTGATCACCACTTCGTTCTCTGGGGTTAAAAACAAGTCTACACGCGCCATGCCTGCGCATCCCAACGTTTGATAAGCCTGAACGGCAATCGc        <  2:273271/140‑1 (MQ=255)                                                                                                                                  ggATACATGCTGATATTAGTGAAGCCAGGCAGGGTGTTGATCTCGTTGATCACCACTTCGTTCTCTGGGGTTAAAAACACGTCTACACGCGCCATGCCTGCGCATCCCAACGTTTGATAAGCCTGAACGGCAATCGCCCg     >  2:1260544/1‑140 (MQ=255)                                                                                                                                     tacatgcTGATATTAGTGAAGCCAGGCAGGGTGTTGATCTCGTTGATCACCACTTCGTTCTCTGGGGTTAAAAACACGTCTACACGCGCCATGCCTGCGCATCCCAACGTTTGATAAGCCTGAACGGCAATCGCCCGGAt  <  1:1068487/140‑1 (MQ=255)                                                                                                                                          |                                                                                                                                       TATTATCGGGCGTTAAAATATTACATTGTGGTTTTCAATGCGTTATCCGCAGCGTGACGCTCCAGCGCCAGTTCAATCAAACGTGTGATCAGATCGGTGTAACCCAGACCGCTGGCTTGCCACAGCTTCGGATACATACTGATATTAGTGAAGCCAGGCAGGGTGTTGATCTCGTTGATCACCACTTCGTTCTCTGGGGTTAAAAACACGTCTACACGCGCCATGCCTGCGCATCCCAACGTTTGATAAGCCTGAACGGCAATCGCCCGGAT  >  NC_000913/399809‑400080

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑