Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,140,368	T→C	V144V (GTA→GTG)	hybE ←	hydrogenase 2‑specific chaperone

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,140,368	0	T	C	100.0%	278.9 / NA	75	V144V (GTA→GTG)	hybE	hydrogenase 2‑specific chaperone
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (42/33);  total (42/33)

GTGACGCGCTTAACATCGTGCTGCTCCGCCTGCCGTTGGATAATTTCAACGGCGCTCTGGCAAAGAGACAACTCATGCATTTTCGCCACTCCTGCGACCAAACAGCAGGGCGCGACGCCCTGCGTGTGGTACATCCGGATTCGTGACTGGCAGCGAAAGGATCATTCGTGCGCAGTCATCCGTCAGGCGTTGGCCCTCTTCAATCGACATGCTGTGCGAAAGCGGCGACATCAGCGAACAGGAGAGATATTGCGAAACA  >  NC_000913/3140239‑3140497                                                                                                                                  |                                                                                                                                  gTGACGCGCTTAACATCGTGCTGCTCCGCCTGCCGTTGGATAATTTCAACGGCGCTCTGGCAAAGAGACAACTCATGCATTTTCGCCACTCCTGCGACCAAACAGCAGGGCGCGACGCCCTGCGTGTGGCACATCCGGAt                                                                                                                         >  2:40558/1‑140 (MQ=255) gTGACGCGCTTAACATCGTGCTGCTCCGCCTGCCGTTGGATAATTTCAACGGCGCTCTGGCAAAGAGACAACTCATGCATTTTCGCCACTCCTGCGACCAAACAGCAGGGCGCGACGCCCTGCGTGTGGCACATCCGGAt                                                                                                                         >  2:251727/1‑140 (MQ=255)    aCGCGCTTAACATCGTGCTGCTCCGCCTGCCGTTGGATAATTTCAACGGCGCTCTGGCAAAGAGACAACTCATGCATTTTCGCCACTCCTGCGACCAAACAGCAGGGCGCGACGCCCTGCGTGTGGCACATCCGGATTCg                                                                                                                      >  2:608512/1‑140 (MQ=255)         cTTAACATCGTGCTGCTCCGCCTGCCGTTGGATAATTTCAACGGCGCTCTGGCAAAGAGACAACTCATGCATTTTCGCCACTCCTGCGACCAAACAGCAGGGCGCGACGCCCTGCGTGTGGCACATc                                                                                                                              <  1:373272/127‑1 (MQ=255)         cTTAACATCGTGCTGCTCCGCCTGCCGTTGGATAATTTCAACGGCGCTCTGGCAAAGAGACAACTCATGCATTTTCGCCACTCCTGCGACCAAACAGCAGGGCGCGACGCCCTGCGTGTGGCACATc                                                                                                                              >  2:373272/1‑127 (MQ=255)             aCATCGTGCTGCTCCGCCTGCCGTTGGATAATTTCAACGGCGCTCTGGCAAAGAGACAACTCATGCATTTTCGCCACTCCTGCGACCAAACAGCAGGGCGCGACGCCCTGCGTGTGGCACATCCGGATTCGTGACTGGCa                                                                                                             <  2:663953/140‑1 (MQ=255)                tCGTGCTGCTCCGCCTGCCGTTGGATAATTTCAACGGCGCTCTGGCAAAGAGACAACTCATGCATTTTCGCCACTCCTGCGACCAAACAGCAGGGCGCGACGCCCTGCGTGTGGCACATCCGGATTCGTGACTGGCAGCg                                                                                                          <  2:977188/140‑1 (MQ=255)                 cGTGCTGCTCCGCCTGCCGTTGGATAATTTCAACGGCGCTCTGGCAAAGAGACAACTCATGCATTTTCGCCACTCCTGCGACCAAACAGCAGGGCGCGACGCCCTGCGTGTGGCACATCCGGATTCGTGACTGGCAGCGa                                                                                                         >  1:151546/1‑140 (MQ=255)                   tgctgcTCCGCCTGCCGTTGGATAATTTCAACGGTGCTCTGGCAAAGAGACAACTCATGCATTTTCGCCACTCCTGCGACCAAACAGCAGGGCGCGACGCCCTGCGTGTGGCACATCCGGATTCGTGACTGGCAGCGaaa                                                                                                       >  1:1356445/1‑140 (MQ=255)                   tgctgcTCCGCCTGCCGTTGGATAATTTCAACGGCGCTCTGGCAAAGAGACAACTCATGCATTTTCGCCACTCCTGCGACCAAACAGCAGGGCGCGACGCCCTGCGTGTGGCACATCCGGATTCGTGACTgg                                                                                                               >  1:1158835/1‑132 (MQ=255)                   tgctgcTCCGCCTGCCGTTGGATAATTTCAACGGCGCTCTGGCAAAGAGACAACTCATGCATTTTCGCCACTCCTGCGACCAAACAGCAGGGCGCGACGCCCTGCGTGTGGCACATCCGGATTCGTGACTgg                                                                                                               <  2:1158835/132‑1 (MQ=255)                   tgctgcTCCGCCTGCCGTTGGATAATTTCAACGGCGCTCTGGCAAAGAGACAACTCATGCATTTTCGCCACTCCTGCGACCAAACAGCAGGGCGCGACGCCCTGCGTGTGGCACATCCGGATTCGTGACTGGCAGCGaaa                                                                                                       >  1:1295450/1‑140 (MQ=255)                    gctgctCCGCCTGCCGTTGGATAATTTCAACGGCGCTCTGGCAAAGAGACAACTCATGCATTTTCGCCACTCCTGCGACCAAACAGCAGGGCGCGACGCCCTGCGTGTGGCACATCCGGATTCGTGACTGGCAGCGAAAg                                                                                                      >  2:787150/1‑140 (MQ=255)                    gctgctCCGCCTGCCGTTGGATAATTTCAACGGCGCTCTGGCAAAGAGACAACTCATGCATTTTCGCCACTCCTGCGACCAAACAGCAGGGCGCGACGCCCTGCGTGTGGCACATCCGGATTCGTGACTGGCAGCGAAAg                                                                                                      >  1:1396914/1‑140 (MQ=255)                    gctgctCCGCCTGCCGTTGGATAATTTCAACGGCGCTCTGGCAAAGAGACAACTCATGCATTTTCGCCACTCCTGCGACCAAACAGCAGGGCGCGACGCCCTGCGTGTGGCACATCCGGATTCGTGACTGGCAGCGAAAg                                                                                                      >  1:1048194/1‑140 (MQ=255)                        ctCCGCCTGCCGTTGGATAATTTCAACGGCGCTCTGGCAAAGAGACAACTCATGCATTTTCGCCACTCCTGCGACCAAACAGCAGGGCGCGACGCCCTGCGTGTGGCACATCCGGATTCGTGACTGGCAGCGAAAGGATc                                                                                                  >  1:698839/1‑140 (MQ=255)                          ccGCCTGCCGTTGGATAATTTCAACGGCGCTCTGGCAAAGAGACAACTCATGCATTTTCGCCACTCCTGCGACCAAACAGCAGGGCGCGACGCCCTGCGTGTGGCACATCCGGATTCGTGACTGGCAGCGAAAGGATCAt                                                                                                >  1:1183028/1‑140 (MQ=255)                            gCCTGCCGTTGGATAATTTCAACGGCGCTCTGGCAAAGAGACAACTCATGCATTTTCGCCACTCCTGCGACCAAACAGCAGGGCGCGACGCCCTGCGTGTGGCACATCCGGATTCGTGACTGGCAGCGAAAGGATCATTc                                                                                              <  1:1228726/140‑1 (MQ=255)                             ccTGCCGTTGGATAATTTCAACGGCGCTCTGGCAAAGAGACAACTCATGCATTTTCGCCACTCCTGCGACCAAACAGCAGGGCGCGACGCCCTGCGTGTGGCACATCCGGATTCGTGACTGGCAGCGAAAGGATCATTCg                                                                                             >  2:15737/1‑140 (MQ=255)                                  cGTTGGATAATTTCAACGGCGCTCTGGCAAAGAGACAACTCATGCATTTTCGCCACTCCTGCGACCAAACAGCAGGGCGCGACGCCCTGCGTGTGGCACATCCGGATTCGTGACTGGCAGCGAAAGGATCATTCGTccgc                                                                                        >  2:1402097/1‑140 (MQ=255)                                            tttCAACGGCGCTCTGGCAAAGAGACAACTCATGCATTTTCGCCACTCCTGCGACCAAACAGCAGGGCGCGACGCCCTGCGTGTGGCACATCCGGATTCGTGACTGGCAGCGAAAGGATCATTCGTGCGCAGTCATCCGt                                                                              >  1:436995/1‑140 (MQ=255)                                                aaCGGCGCTCTGGCAAAGAGACAACTCATGCATTTTCGCCACTCCTGCGACCAAACAGCAGGGCGCGACGCCCTGCGTGTGGCACATCCGGATTCGTGACTGGCAGCGAAAGGATCATTCGTGCGCAGTCATCCGTCAgg                                                                          <  1:1202054/140‑1 (MQ=255)                                                 aCGGCGCTCTGGCAAAGAGACAACTCATGCATTTTCGCCACTCCTGCGACCAAACAGCAGGGCGCGACGCCCTGCGTGTGGCACATCCGGATTCGTGACTGGCAGCGAAAGGATCATTCGTGCGCAGTCATCCGTCAGGc                                                                         >  1:1004705/1‑140 (MQ=255)                                                      gcTCTGGCAAAGAGACAACTCATGCATTTTCGCCACTCCTGCGACCAAACAGCAGGGCGCGACGCCCTGCGTGTGGCACATCCGGATTCGTGACTGGCAGCGAAAGGATCATTCGTGCGCAGTCATCCGTCAGGCGTTgg                                                                    <  2:698839/140‑1 (MQ=255)                                                      gcTCTGGCAAAGAGACAACTCATGCATTTTCGCCACTCCTGCGACCAAACAGCAGGGCGCGACGCCCTGCGTGTGGCACATCCGGATTCGTGACTGGCAGCGAAAGGATCATTCGTGCGCAGTCATCCGTCAGGCGTTgg                                                                    >  1:635066/1‑140 (MQ=255)                                                      gcTCTGGCAAAGAGACAACTCATGCATTTTCGCCACTCCTGCGACCAAACAGCAGGGCGCGACGCCCTGCGTGTGGCACATCCGGATTCGTGACTGGCAGCGAAAGGATCATTCGTGCGCAGTCATCCGTCAGGCGTTgg                                                                    <  1:679443/140‑1 (MQ=255)                                                       ctctGGCAAAGAGACAACTCATGCATTTTCGCCACTCCTGCGACCAAACAGCAGGGCGCGACGCCCTGCGTGTGGCACATCCGGATTCGTGACTGGCAGCGAAAGGATCATTCGTGCGCAGTCATCCGTCAGGCGTTGGc                                                                   >  1:994927/1‑140 (MQ=255)                                                       ctctGGCAAAGAGACAACTCATGCATTTTCGCCACTCCTGCGACCAAACAGCAGGGCGCGACGCCCTGCGTGTGGCACATCCGGATTCGTGACTGGCAGCGAAAGGATCATTCGTGCGCAGTCATCCGTCAGGCGTTGGc                                                                   >  1:514870/1‑140 (MQ=255)                                                       ctctGGCAAAGAGACAACTCATGCATTTTCGCCACTCCTGCGACCAAACAGCAGGGCGCGACGCCCTGCGTGTGGCACATCCGGATTCGTGACTGGCAGCGAAAGGATCATTCGTGCGCAGTCATCCGTCAGGCGTTGGc                                                                   >  1:564535/1‑140 (MQ=255)                                                       ctctGGCAAAGAGACAACTCATGCATTTTCGCCACTCCTGCGACCAAACAGCAGGGCGCGACGCCCTGCGTGTGGCACATCCGGATTCGTGACTGGCAGCGAAAGGATCATTCGTGCGCAGTCATCCGTCAGGCGTTGGc                                                                   >  1:1173033/1‑140 (MQ=255)                                                       ctctGGCAAAGAGACAACTCATGCATTTTCGCCACTCCTGCGACCAAACAGCAGGGCGCGACGCCCTGCGTGTGGCACATCCGGATTCGTGACTGGCAGCGAAAGGATCATTCGTGCGCAGTCATCCGTCAGGCGTTGGc                                                                   >  2:123269/1‑140 (MQ=255)                                                            gCAAAGAGACAACTCATGCATTTTCGCCACTCCTGCGACCAAACAGCAGGGCGCGACGCCCTGCGTGTGGCACATCCGGATTCGTGACTGGCAGCGAAAGGATCATTCGTGCGCAGTCATCCGTCAGGCGTTGGCCctct                                                              >  2:891785/1‑140 (MQ=255)                                                             cAAAGAGACAACTCATGCATTTTCGCCACTCCTGCGACCAAACAGCAGGGCGCGACGCCCTGCGTGTGGCACATCCGGATTCGTGACTGGCAGCGAAAGGATCATTCGTGCGCAGTCATCCGTCAGGCGTTGGCCCTCtt                                                             <  2:1048194/140‑1 (MQ=255)                                                             cAAAGAGACAACTCATGCATTTTCGCCACTCCTGCGACCAAACAGCAGGGCGCGACGCCCTGCGTGTGGCACATCCGGATTCGTGACTGGCAGCGAAAGGATCATTCGTCCGCAGTCATCCGTCAGGCGTTGGCCCTCtt                                                             <  1:1402097/140‑1 (MQ=255)                                                              aaaGAGACAACTCATGCATTTTCGCCACTCCTGCGACCAAACAGCAGGGCGCGACGCCCTGCGTGTGGCACATCCGGATTCGTGACTGGCAGCGAAAGGATCATTCGTGCGCAGTCATCCGTCAGGCGTTGGCCCTCTTc                                                            <  1:1400619/140‑1 (MQ=255)                                                              aaaGAGACAACTCATGCATTTTCGCCACTCCTGCGACCAAACAGCAGGGCGCGACGCCCTGCGTGTGGCACATCCGGATTCGTGACTGGCAGCGAAAGGATCATTCGTGCGCAGTCATCCGTCAGGCGTTGGCCCTCTTc                                                            <  1:891785/140‑1 (MQ=255)                                                              aaaGAGACAACTCATGCATTTTCGCCACTCCTGCGACCAAACAGCAGGGCGCGACGCCCTGCGTGTGGCACATCCGGATTCGTGACTGGCAGCGAAAGGATCATTCGTGCGCAGTCATCCGTCAGGCGTTGGCCCTCTTc                                                            <  2:1396914/140‑1 (MQ=255)                                                              aaaGAGACAACTCATGCATTTTCGCCACTCCTGCGACCAAACAGCAGGGCGCGACGCCCTGCGTGTGGCACATCCGGATTCGTGACTGGCAGCGAAAGGATCATTCGTGCGCAGTCATCCGTCAGGCGTTGGCCCTCTTc                                                            <  1:15737/140‑1 (MQ=255)                                                                agagACAACTCATGCATTTTCGCCACTCCTGCGACCAAACAGCAGGGCGCGACGCCCTGCGTGTGGCACATCCGGATTCGTGACTGGCAGCGAAAGGATCATTCGTGCGCAGTCATCCGTCAGGCGTTGGCCCTCTTCaa                                                          <  2:151546/140‑1 (MQ=255)                                                                    aCAACTCATGCATTTTCGCCACTCCTGCGACCAAACAGCAGGGCGCGACGCCCTGCGTGTGGCACATCCGGATTCGTGACTGGCAGCGAAAGGATCATTCGTGCGCAGTCATCCGTCAGGCGTTGGCCCTCTTCAATCGa                                                      >  2:168367/1‑140 (MQ=255)                                                                     cAACTCATGCATTTTCGCCACTCCTGCGACCAAACAGCAGGGCGCGACGCCCTGCGTGTGGCACATCCGGATTCGTGACTGGCAGCGAAAGGATCATTCGTGCGCAGTCATCCGTCAGGCGTTGGCCCTCTTCAATCGAc                                                     <  1:787150/140‑1 (MQ=255)                                                                      aaCTCATGCATTTTCGCCACTCCTGCGACCAAACAGCAGGGCGCGACGCCCTGCGTGTGGCACATCCGGATTCGTGACTGGCAGCGAAAGGATCATTCGTGCGCAGTCATCCGTCAGGCGTTGGCCCTCTTCAATCGACa                                                    >  2:1039479/1‑140 (MQ=255)                                                                      aaCTCATGCATTTTCGCCACTCCTGCGACCAAACAGCAGGGCGCGACGCCCTGCGTGTGGCACATCCGGATTCGTGACTGGCAGCGAAAGGATCATTCGTGCGCAGTCATCCGTCAGGCGTTGGCCCTCTTCAATCGACa                                                    >  2:433519/1‑140 (MQ=255)                                                                      aaCTCATGCATTTTCGCCACTCCTGCGACCAAACAGCAGGGCGCGACGCCCTGCGTGTGGCACATCCGGATTCGTGACTGGCAGCGAAAGGATCATTCGTGCGCAGTCATCCGTCAGGCGTTGGCCCTCTTCAATCGACa                                                    >  1:642528/1‑140 (MQ=255)                                                                      aaCTCATGCATTTTCGCCACTCCTGCGACCAAACAGCAGGGCGCGACGCCCTGCGTGTGGCACATCCGGATTCGTGACTGGCAGCGAAAGGATCATTCGTGCGCAGTCATCCGTCAGGCGTTGGCCCTCTTCAATCGACa                                                    <  1:608512/140‑1 (MQ=255)                                                                      aaCTCATGCATTTTCGCCACTCCTGCGACCAAACAGCAGGGCGCGACGCCCTGCGTGTGGCACATCCGGATTCGTGACTGGCAGCGAAAGGATCATTCGTGCGCAGTCATCCGTCAGGCGTTGGCCCTCTTCAATCGACa                                                    >  1:1022240/1‑140 (MQ=255)                                                                           aTGCATTTTCGCCACTCCTGCGACCAAACAGCAGGGCGCGACGCCCTGCGTGTGGCACATCCGGATTCGTGACTGGCAGCGAAAGGATCATTCGTGCGCAGTCATCCGTCAGGCGTTGGCCCTCTTCAATCGACATGCtg                                               >  2:496555/1‑140 (MQ=255)                                                                                  ttCGCCACTCCTGCGACCAAACAGCAGGGCGCGACGCCCTGCGTGTGGCACATCCGGATTCGTGACTGGCAGCGAAAGGATCATTCGTGCGCAGTCATCCGTCAGGCGTTGGCCCTCTTCAATCGACATGCTGTGCGaaa                                        <  2:1356445/140‑1 (MQ=255)                                                                                  ttCGCCACTCCTGCGACCAAACAGCAGGGCGCGACGCCCTGCGTGTGGCACATCCGGATTCGTGACTGGCAGCGAAAGGATCATTCGTGCGCAGTCATCCGTCAGGCGTTGGCCCTCTTCAATCGACATGCTGTGCGaaa                                        >  1:154026/1‑140 (MQ=255)                                                                                   tCGCCACTCCTGCGACCAAACAGCAGGGCGCGACGCCCTGCGTGTGGCACATCCGGATTCGTGACTGGCAGCGAAAGGATCATTCGTGCGCAGTCATCCGTCAGGCGTTGGCCCTCTTCAATCGACATGCTGTGCGAAAg                                       >  2:421136/1‑140 (MQ=255)                                                                                   tCGCCACTCCTGCGACCAAACAGCAGGGCGCGACGCCCTGCGTGTGGCACATCCGGATTCGTGACTGGCAGCGAAAGGATCATTCGTGCGCAGTCATCCGTCAGGCGTTGGCCCTCTTCAATCGACATGCTGTGCGAAAg                                       >  1:757699/1‑140 (MQ=255)                                                                                         cTCCTGCGACCAAACAGCAGGGCGCGACGCCCTGCGTGTGGCACATCCGGATTCGTGACTGGCAGCGAAAGGATCATTCGTGCGCAGTCATCCGTCAGGCGTTGGCCCTCTTCAATCGACATGCTGTGCGAAAGCGGCGa                                 <  2:154026/140‑1 (MQ=255)                                                                                          tCCTGCGACCAAACAGCAGGGCGCGACGCCCTGCGTGTGGCACATCCGGATTCGTGACTGGCAGCGAAAGGATCATTCGTGCGCAGTCATCCGTCAGGCGTTGGCCCTCTTCAATCGACATGCTGTGCGAAAGCGGCGAc                                >  2:1376064/1‑140 (MQ=255)                                                                                              gCGACCAAACAGCAGGGCGCGACGCCCTGCGTGTGGCACATCCGGATTCGTGACTGGCAGCGAAAGGATCATTCGTGCGCAGTCATCCGTCAGGCGTTGGCCCTCTTCAATCGACATGCTGTGCGAAAGCGGCGACATCa                            <  2:757699/140‑1 (MQ=255)                                                                                              gCGACCAAACAGCAGGGCGCGACGCCCTGCGTGTGGCACATCCGGATTCGTGACTGGCAGCGAAAGGATCATTCGTGCGCAGTCATCCGTCAGGCGTTGGCCCTCTTCAATCGACATGCTGTGCGAAAGCGGCGACATCa                            <  2:994927/140‑1 (MQ=255)                                                                                                  ccAAACAGCAGGGCGCGACGCCCTGCGTGTGGCACATCCGGATTCGTGACTGGCAGCGAAAGGATCATTCGTGCGCAGTCATCCGTCAGGCGTTGGCCCTCTTCAATCGACATGCTGTGCGAAAGCGGCGACATCAGCGa                        <  1:168367/140‑1 (MQ=255)                                                                                                   cAAACAGCAGGGCGCGACGCCCTGCGTGTGGCACATCCGGATTCGTGACTGGCAGCGAAAGGATCATTCGTGCGCAGTCATCCGTCAGGCGTTGGCCCTCTTCAATCGACATGCTGTGCGAAAGCGGCGACATCAGCGaa                       <  2:1183028/140‑1 (MQ=255)                                                                                                    aaaCAGCAGGGCGCGACGCCCTGCGTGTGGCACATCCGGATTCGTGACTGGCAGCGAAAGGATCATTCGTGCGCAGTCATCCGTCAGGCGTTGGCCCTCTTCAATCGACATGCTGTGCGAAAGCGGCGACATCAGCGaa                       <  2:470976/139‑1 (MQ=255)                                                                                                    aaaCAGCAGGGCGCGACGCCCTGCGTGTGGCACATCCGGATTCGTGACTGGCAGCGAAAGGATCATTCGTGCGCAGTCATCCGTCAGGCGTTGGCCCTCTTCAATCGACATGCTGTGCGAAAGCGGCGACATCAGCGaa                       >  1:470976/1‑139 (MQ=255)                                                                                                    aaaCAGCAGGGCGCGACGCCCTGCGTGTGGCACATCCGGATTCGTGACTGGCAGCGAAAGGATCATTCGTGCGCAGTCATCCGTCAGGCGTTGGCCCTCTTCAATCGACATGCTGTGCGAAAGCGGCGACATCAGCGAAc                      <  2:1173033/140‑1 (MQ=255)                                                                                                      aCAGCAGGGCGCGACGCCCTGCGTGTGGCACATCCGGATTCGTGACTGGCAGCGAAAGGATCATTCGTGCGCAGTCATCCGTCAGGCGTTGGCCCTCTTCAATCGACATGCTGTGCGAAAGCGGCGACATCAGCGAACAg                    >  2:165928/1‑140 (MQ=255)                                                                                                        agcagGGCGCGACGCCCTGCGTGTGGCACATCCGGATTCGTGACTGGCAGCGAAAGGATCATTCGTGCGCAGTCATCCGTCAGGCGTTGGCCCTCTTCAATCGACATGCTGTGCGAAAGCGGCGACATCAGCGAACAGga                  <  1:40558/140‑1 (MQ=255)                                                                                                             ggCGCGACGCCCTGCGTGTGGCACATCCGGATTCGTGACTGGCAGCGAAAGGATCATTCGTGCGCAGTCATCCGTCAGGCGTTGGCCCTCTTCAATCGACATGCTGTGCGAAAGCGGCGACATCAGCGaa                       >  1:711495/1‑130 (MQ=255)                                                                                                             ggCGCGACGCCCTGCGTGTGGCACATCCGGATTCGTGACTGGCAGCGAAAGGATCATTCGTGCGCAGTCATCCGTCAGGCGTTGGCCCTCTTCAATCGACATGCTGTGCGAAAGCGGCGACATCAGCGaa                       <  2:711495/130‑1 (MQ=255)                                                                                                             ggCGCGACGCCCTGCGTGTGGCACATCCGGATTCGTGACTGGCAGCGAAAGGATCATTCGTGCGCAGTCATCCGTCAGGCGTTGGCCCTCTTCAATCGACATGCTGTGCGAAAGCGGCGACATCAGCGAACAGGAGAGat             >  1:135600/1‑140 (MQ=255)                                                                                                              gcgcGACGCCCTGCGTGTGGCACATCCGGATTCGTGACTGGCAGCGAAAGGATCATTCGTGCGCAGTCATCCGTCAGGCGTTGGCCCTCTTCAATCGACATGCTGTGCGAAAGCGGCGACATCAGCGAACAGGAGAGata            >  1:127308/1‑140 (MQ=255)                                                                                                              gcgcGACGCCCTGCGTGTGGCACATCCGGATTCGTGACTGGCAGCGAAAGGATCATTCGTGCGCAGTCATCCGTCAGGCGTTGGCCCTCTTCAATCGACATGCTGTGCGAAAGCGGCGACATCAGCGAACAGGAGAGata            <  1:1376064/140‑1 (MQ=255)                                                                                                               cgcgACGCCCTGCGTGTGGCACATCCGGATTCGTGACTGGCAGCGAAAGGATCATTCGTGCGCAGTCATCCGTCAGGCGTTGGCCCTCTTCAATCGACATGCTGTGCGAAAGCGGCGACATCAGCGaa                       <  1:791976/128‑1 (MQ=255)                                                                                                               cgcgACGCCCTGCGTGTGGCACATCCGGATTCGTGACTGGCAGCGAAAGGATCATTCGTGCGCAGTCATCCGTCAGGCGTTGGCCCTCTTCAATCGACATGCTGTGCGAAAGCGGCGACATCAGCGaa                       >  2:791976/1‑128 (MQ=255)                                                                                                                gcgACGCCCTGCGTGTGGCACATCCGGATTCGTGACTGGCAGCGAAAGGATCATTCGTGCGCAGTCATCCGTCAGGCGTTGGCCCTCTTCAATCGACATGCTGTGCGAAAGCGGCGACATCAGCGAACAGGAGAGata            >  2:642007/1‑138 (MQ=255)                                                                                                                gcgACGCCCTGCGTGTGGCACATCCGGATTCGTGACTGGCAGCGAAAGGATCATTCGTGCGCAGTCATCCGTCAGGCGTTGGCCCTCTTCAATCGACATGCTGTGCGAAAGCGGCGACATCAGCGAACAGGAGAGata            <  1:642007/138‑1 (MQ=255)                                                                                                                   aCGCCCTGCGTGTGGCACATCCGGATTCGTGACTGGCAGCGAAAGGATCATTCGTGCGCAGTCATCCGTCAGGCGTTGGCCCTCTTCAATCGACATGCTGTGCGAAAGCGGCGACATCAGCGAACAGGAGAGATATTGct       <  2:415876/140‑2 (MQ=255)                                                                                                                   aCGCCCTGCGTGTGGCACATCCGGATTCGTGACTGGCAGCGAAAGGATCATTCGTGCGCAGTCATCCGTCAGGCGTTGGCCCTCTTCAATCGACATGCTGTGCGAAAGCGGCGACATCAGCGAACAGGAGAGATATTGCg       <  1:251727/140‑1 (MQ=255)                                                                                                                   aCGCCCTGCGTGTGGCACATCCGGATTCGTGACTGGCAGCGAAAGGATCATTCGTGCGCAGTCATCCGTCAGGCGTTGGCCCTCTTCAATCGACATGCTGTGCGAAAGCGGCGACATCAGCGAACAGGAGAGATATTGCg       <  2:1295450/140‑1 (MQ=255)                                                                                                                        cTGCGTGTGGCACATCCGGATTCGTGACTGGCAGCGAAAGGATCATTCGTGCGCAGTCATCCGTCAGGCGTTGGCCCTCTTCAATCGACATGCTGTGCGAAAGCGGCGACATCAGCGAACAGGAGAGATATTGCGAAaca  >  1:483977/1‑140 (MQ=255)                                                                                                                                  |                                                                                                                                  GTGACGCGCTTAACATCGTGCTGCTCCGCCTGCCGTTGGATAATTTCAACGGCGCTCTGGCAAAGAGACAACTCATGCATTTTCGCCACTCCTGCGACCAAACAGCAGGGCGCGACGCCCTGCGTGTGGTACATCCGGATTCGTGACTGGCAGCGAAAGGATCATTCGTGCGCAGTCATCCGTCAGGCGTTGGCCCTCTTCAATCGACATGCTGTGCGAAAGCGGCGACATCAGCGAACAGGAGAGATATTGCGAAACA  >  NC_000913/3140239‑3140497

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑