Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,209,866	G→A	G226G (GGC→GGT)	tsaD ←	N(6)‑L‑threonylcarbamoyladenine synthase, TsaD subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,209,866	0	G	A	100.0%	192.2 / NA	57	G226G (GGC→GGT)	tsaD	N(6)‑L‑threonylcarbamoyladenine synthase, TsaD subunit
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (35/22);  total (35/22)

TAGCACTCACGCCGCCCGCCATGACCAGTCGCTTAAAGCCCGTCTGATCCAGCGCCCGCTTGCACTTAATCATCAGCGTATCGACCACCGCATCTTCAAAGGCGCGGGCGATATCAGCACGCGTCTGGTCGTCGGTGCCGTTGTCACGAATGGTATTTGCCGCGAAGGTTTTCAGGCCGGAGAAGCTGAAATCCAGCCCCGGACGGTCGGTCATCGGACGCGGGAAGACAAAGCGCCCGGCAGTACCCTGAGCCGCCATTTTCGACAGTAACG  >  NC_000913/3209730‑3210002                                                                                                                                         |                                                                                                                                         tAGCACTCACGCCGCCCGCCATGACCAGTCGCTTAAAGCCCGTCTGATCCAGCGCCCGCTTGCACTTAATCATCAGCGTATCGACCACCGCATCTTCAAAGGCGCGGGCGATATCAGCACGCGTCTGGTCGTCGGTACCg                                                                                                                                       >  1:1339830/1‑140 (MQ=255)   gcccccACGCCGCCCGCCATGACCAGTCGCTTAAAGCCCGTCTGATCCAGCGCCCGCTTGCACTTAATCATCAGCGTATCGACCACCGCATCTTCAAAGGCGCGGGCGATATCAGCACGCGTCTGGTCGTCGGTACCGtt                                                                                                                                     <  1:583253/135‑1 (MQ=255)        cACGCCGCCCGCCATGACCAGTCGCTTAAAGCCCGTCTGATCCAGCGCCCGCTTGCACTTAATCATCAGCGTATCGACCACCGCATCTTCAAAGGCGCGGGCGATATCAGCACGCGTCTGGTCGTCGGTACCGTTGTCAc                                                                                                                                >  2:233758/1‑140 (MQ=255)          cgccgcCCGCCATGACCAGTCGCTTAAAGCCCGTCTGATCCAGCGCCCGCTTGCACTTAATCATCAGCGTATCGACCACCGCATCTTCAAAGGCGCGGGCGATATCAGCACGCGTCTGGTCGTCGGTACCGTTGTCACGa                                                                                                                              >  2:370998/1‑140 (MQ=255)          cgccgcCCGCCATGACCAGTCGCTTAAAGCCCGTCTGATCCAGCGCCCGCTTGCACTTAATCATCAGCGTATCGACCACCGCATCTTCAAAGGCGCGGGCGATATCAGCACGCGTCTGGTCGTCGGTACCGTTGTCACGa                                                                                                                              >  2:35407/1‑140 (MQ=255)                             tCGCTTAAAGCCCGTCTGATCCAGCGCCCGCTTGCACTTAATCATCAGCGTATCGACCACCGCATCTTCAAAGGCGCGGGCGATATCAGCACGCGTCTGGTCGTCGGTACCGTTGTCACCAATGGTATTTGCCGCGAAgg                                                                                                           >  1:929599/1‑140 (MQ=255)                                      gCCCGTCTGATCCAGCGCCCGCTTGCACTTAATCATCAGCGTATCGACCACCGCATCTTCAAAGGCGCGGGCGATATCAGCACGCGTCTGGTCGTCGGTACCGTTGTCACGAATGGTATTTGCCGCGAAGGTTTTCAGGc                                                                                                  <  2:1339830/140‑1 (MQ=255)                                           tCTGATCCAGCGCCCGCTTGCACTTAATCATCAGCGTATCGACCACCGCATCTTCAAAGGCGCGGGCGATATCAGCACGCGTCTGGTCGTCGGTACCGTTGTCACGAATGGTATTTGCCGCGAAGGTTTTCAGGCCGgag                                                                                             >  2:1081075/1‑140 (MQ=255)                                            cTGATCCAGCGCCCGCTTGCACTTAATCATCAGCGTATCGACCACCGCATCTTCAAAGGCGCGGGCGATATCAGCACGCGTCTGGTCGTCGGTACCGTTGTCACGAATGGTATTTGCCGCGAAGGTTTTCAGGCCGgaga                                                                                            <  2:526884/140‑1 (MQ=255)                                                  cAGCGCCCGCTTGCACTTAATCATCAGCGTATCGACCACCGCATCTTCAAAGGCGCGGGCGATATCAGCACGCGTCTGGTCGTCGGTACCGTTGTCACGAATGGTATTTGCCGCGAAGGTTTTCAGGCCGGAGAAGCTGa                                                                                      >  1:1405041/1‑140 (MQ=255)                                                     cgcCCGCTTGCACTTAATCATCAGCGTATCGACCACCGCATCTTCAAAGGCGCGGGCGATATCAGCACGCGTCTGGTCGTCGGTACCGTTGTCACGAATGGTATTTGCCGCGAAGGTTTTCAGGCCGGAGAAGCTGAAAt                                                                                   <  1:35407/140‑1 (MQ=255)                                                           cTTGCACTTAATCATCAGCGTATCGACCACCGCATCTTCAAAGGCGCGGGCGATATCAGCACGCGTCTGGTCGTCGGTACCGTTGTCACGAATGGTATTTGCCGCGAAGGTTTTCAGGCCGGAGAAGCTGAAATCCAGcc                                                                             >  2:283187/1‑140 (MQ=255)                                                                  ttAATCATCAGCGTATCGACCACCGCATCTTCAAAGGCGCGGGCGATATCAGCACGCGTCTGGTCGTCGGTACCGTTGTCACGAATGGTATTTGCCGCGAAGGTTTTCAGGCCGGAGAAGCTGAAATCCAGCCCCGGAcg                                                                      >  1:17868/1‑140 (MQ=255)                                                                    aaTCATCAGCGTATCGACCACCGCATCTTCAAAGGCGCGGGCGATATCAGCACGCGTCTGGTCGTCGGTACCGTTGTCACGAATGGTATTTGCCGCGAAGGTTTTCAGGCCGGAGAAGCTGAAATCCAGCCCCGGAcggt                                                                    >  2:1060130/1‑140 (MQ=255)                                                                    aaTCATCAGCGTATCGACCACCGCATCTTCAAAGGCGCGGGCGATATCAGCACGCGTCTGGTCGTCGGTACCGTTGTCACGAATGGTATTTGCCGCGAAGGTTTTCAGGCCGGAGAAGCTGAAATCCAGCCCCGGAcggt                                                                    <  1:99924/140‑1 (MQ=255)                                                                    aaTCATCAGCGTATCGACCACCGCATCTTCAAAGGCGCGGGCGATATCAGCACGCGTCTGGTCGTCGGTACCGTTGTCACGAATGGTATTTGCCGCGAAGGTTTTCAGGCCGGAGAAGCTGAAATCCAGCCCCGGAcggt                                                                    <  1:370998/140‑1 (MQ=255)                                                                          caGCGTATCGACCACCGCATCTTCAAAGGCGCGGGCGATATCAGCACGCGTCTGGTCGTCGGTACCGTTGTCACGAATGGTATTTGCCGCGAAGGTTTTCAGGCCGGAGAAGCTGAAATCCAGCCCCGGACGGTCGGTCa                                                              >  1:893014/1‑140 (MQ=255)                                                                            gCGTATCGACCACCGCATCTTCAAAGGCGCGGGCGATATCAGCACGCGTCTGGTCGTCGGTACCGTTGTCACGAATGGTATTTGCCGCGAAGGTTTTCAGGCCGGAGAAGCTGAAATCCAGccc                                                                            <  2:1283037/124‑1 (MQ=255)                                                                            gCGTATCGACCACCGCATCTTCAAAGGCGCGGGCGATATCAGCACGCGTCTGGTCGTCGGTACCGTTGTCACGAATGGTATTTGCCGCGAAGGTTTTCAGGCCGGAGAAGCTGAAATCCAGccc                                                                            >  1:1283037/1‑124 (MQ=255)                                                                                aTCGACCACCGCATCTTCAAAGGCGCGGGCGATATCAGCACGCGTCTGGTCGTCGGTACCGTTGTCACGAATGGTATTTGCCGCGAAGGTTTTCAGGCCGGAGAAGCTGAAATCCAGCCCCGGAcggt                                                                    <  2:1210988/128‑1 (MQ=255)                                                                                aTCGACCACCGCATCTTCAAAGGCGCGGGCGATATCAGCACGCGTCTGGTCGTCGGTACCGTTGTCACGAATGGTATTTGCCGCGAAGGTTTTCAGGCCGGAGAAGCTGAAATCCAGCCCCGGAcggt                                                                    >  1:1210988/1‑128 (MQ=255)                                                                                    accaccGCATCTTCAAAGGCGCGGGCGATATCAGCACGCGTCTGGTCGTCGGTACCGTTGTCACGAATGGTATTTGCCGCGAAGGTTTTCAGGCCGGAGAAGCTGAAATCCAGCCCCGGACGGTCGGTCATCGGACGCgg                                                    >  2:186696/1‑140 (MQ=255)                                                                                         cGCATCTTCAAAGGCGCGGGCGATATCAGCACGCGTCTGGTCGTCGGTACCGTTGTCACGAATGGTATTTGCCGCGAAGGTTTTCAGGCCGGAGAAGCTGAAATCCAGCCCCGGACGGTCGGTCATCGGACGCGGGAAGa                                               >  1:881153/1‑140 (MQ=255)                                                                                           cATCTTCAAAGGCGCTGGCGATATCAGCACGCGTCTGGTCGTCGGTACCGTTGTCACGAATGGTATTTGCCGCGAAGGTTTTCAGGCCGGAGAAGCTGAAATCCAGCCCCGGACGGTCGGTCATCGGACGCGGGAAGACa                                             >  1:989325/1‑140 (MQ=255)                                                                                           cATCTTCAAAGGCGCGGGCGATATCAGCACGCGTCTGGTCGTCGGTACCGTTGTCACGAATGGTATTTGCCGCGAAGGTTTTCAGGCCGGAGAAGCTGAAATCCAGCCCCGGACGGTCGGTCATCGGACGCAGGAAGACa                                             >  2:1421680/1‑140 (MQ=255)                                                                                                tCAAAGGCGCGGGCGATATCAGCACGCGTCTGGTCGTCGGTACCGTTGTCACGAATGGTATTTGCCGCGAAGGTTTTCAGGCCGGAGAAGCTGAAATCCAGCCCCGGACGGTCGGTCATCGGACGCGGGAAGACAAAgcg                                        >  1:184535/1‑140 (MQ=255)                                                                                                 cAAAGGCGCGGGCGATATCAGCACGCGTCTGGTCGTCGGTACCGTTGTCACGAATGGTATTTGCCGCGAAGGTTTTCAGGCCGGAGAAGCTGAAATCCAGCCCCGGACGGTCGGTCATCGGACGCGGGAAGACAAAgcgc                                       <  2:893014/140‑1 (MQ=255)                                                                                                  aaaGGCGCGGGCGATATCAGCACGCGTCTGGTCGTCGGTACCGTTGTCACGAATGGTATTTGCCGCGAAGGTTTTCAGGCCGGAGAAGCTGAAATCCAGCCCCGGACGGTCGGTCATCGGACGCGGGAAGACAAAGCGcc                                      <  2:1405041/140‑1 (MQ=255)                                                                                                  aaaGGCGCGGGCGATATCAGCACGCGTCTGGTCGTCGGTACCGTTGTCACGAATGGTATTTGCCGCGAAGGTTTTCAGGCCGGAGAAGCTGAAATCCAGCCCCGGACGGTCGGTCATCGGACGCGGGAAGACAAAGCGcc                                      <  1:1060130/140‑1 (MQ=255)                                                                                                  aaaGGCGCGGGCGATATCAGCACGCGTCTGGTCGTCGGTACCGTTGTCACGAATGGTATTTGCCGCGAAGGTTTTCAGGCCGGAGAAGCTGAAATCCAGCCCCGGACGGTCGGTCATCGGACGCAGGAAGACAAAGCGcc                                      <  1:1421680/140‑1 (MQ=255)                                                                                                   aaGGCGCGGGCGATATCAGCACGCGTCTGGTCGTCGGTACCGTTGTCACGAATGGTATTTGCCGCGAAGGTTTTCAGGCCGGAGAAGCTGAAATCCAGCCCCGGACGGTCGGTCATCGGACGCGGGAAGACAAAgcgc                                       <  1:1340141/138‑1 (MQ=255)                                                                                                   aaGGCGCGGGCGATATCAGCACGCGTCTGGTCGTCGGTACCGTTGTCACGAATGGTATTTGCCGCGAAGGTTTTCAGGCCGGAGAAGCTGAAATCCAGCCCCGGACGGTCGGTCATCGGACGCGGGAAGACAAAgcgc                                       >  2:1340141/1‑138 (MQ=255)                                                                                                     ggCGCGGGCGATATCAGCACGCGTCTGGTCGTCGGTACCGTTGTCACGAATGGTATTTGCCGCGAAGGTTTTCAGGCCGGAGAAGCTGAAATCCAGCCCCGGACGGTCGGTCATCGGACGCGGGAAGACAAAGCGCCCgg                                   <  2:17868/140‑1 (MQ=255)                                                                                                      gcgcGGGCGATATCAGCACGCGTCTGGTCGTCGGTACCGTTGTCACGAATGGTATTTGCCGCGAAGGTTTTCAGGCCGGAGAAGCTGAAATCCAGCCCCGGACGGTCGGTCATCGGACGCGGGAAGACAAAGCGCCCGGc                                  >  1:1080600/1‑140 (MQ=255)                                                                                                      gcgcGGGCGATATCAGCACGCGTCTGGTCGTCGGTACCGTTGTCACGAATGGTATTTGCCGCGAAGGTTTTCAGGCCGGAGAAGCTGAAATCCAGCCCCGGACGGTCGGTCATCGGACGCGGGAAGACAAAGCGCCCGGc                                  >  1:120394/1‑140 (MQ=255)                                                                                                       cgcgGGCGATATCAGCACGCGTCTGGTCGTCGGTACCGTTGTCACGAATGGTATTTGCCGCGAAGGTTTTCAGGCCGGAGAAGCTGAAATCCAGCCCCGGACGGTCGGTCATCGGACGCGGGAAGACAAAGCGCCCGGCa                                 <  1:1081075/140‑1 (MQ=255)                                                                                                       cgcgGGCGATATCAGCACGCGTCTGGTCGTCGGTACCGTTGTCACGAATGGTATTTGCCGCGAAGGTTTTCAGGCCGGAGAAGCTGAAATCCAGCCCCGGACGGTCGGTCATCGGACGCGGGAAGACAAAGCGCCCGGCa                                 >  1:1176686/1‑140 (MQ=255)                                                                                                       cgcgGGCGATATCAGCACGCGTCTGGTCGTCGGTACCGTTGTCACCAATGGTATTTGCCGCGAAGGTTTTCAGGCCGGAGAAGCTGAAATCCAGCCCCGGACGGTCGGTCATCGGACGCGGGAAGACAAAGCGCCCGGCa                                 <  2:929599/140‑1 (MQ=255)                                                                                                        gcgGGCGATATCAGCACGCGTCTGGTCGTCGGTACCGTTGTCACGAATGGTATTTGCCGCGAAGGTTTTCAGGCCGGAGAAGCTGAAATCCAGCCCCGGACGGTCGGTCATCGGACGCGGGAAGACAAAGCGCCCGGCAg                                >  2:676042/1‑140 (MQ=255)                                                                                                         cgGGCGATATCAGCACGCGTCTGGTCGTCGGTACCGTTGTCACGAATGGTATTTGCCGCGAAGGTTTTCAGGCCGGAGAAGCTGAAATCCAGCCCCGGACGGTCGGTCATCGGACGCGGGAAGACAAAGCGcc                                      <  1:1139868/133‑1 (MQ=255)                                                                                                         cgGGCGATATCAGCACGCGTCTGGTCGTCGGTACCGTTGTCACGAATGGTATTTGCCGCGAAGGTTTTCAGGCCGGAGAAGCTGAAATCCAGCCCCGGACGGTCGGTCATCGGACGCGGGAAGACAAAGCGcc                                      >  2:1139868/1‑133 (MQ=255)                                                                                                         cgGGCGATATCAGCACGCGTCTGGTCGTCGGTACCGTTGTCACGAATGGTATTTGCCGCGAAGGTTTTCAGGCCGGAGAAGCTGAAATCCAGCCCCGGACGGTCGGTCATCGGACGCGGGAAGACAAAGCGCCCGGCAGt                               >  1:1108107/1‑140 (MQ=255)                                                                                                                  tCAGCACGCGTCTGGTCGTCGGTACCGTTGTCACGAATGGTATTTGCCGCGAAGGTTTTCAGGCCGGAGAAGCTGAAATCCAGCCCCGGACGGTCGGTCATCGGACGCGGGAAGACAAAGCGCCCGGCAGTACCCTGAgc                      <  2:858388/140‑1 (MQ=255)                                                                                                                      cACGCGTCTGGTCGTCGGTACCGTTGTCACGAATGGTATTTGCCGCGAAGGTTTTCAGGCCGGAGAAGCTGAAATCCAGCCCCGGACGGTCGGTCATCGGACGCGGGAAGACAAAGCGCCCGGCAGTACCCTGAgccgcc                  >  2:85815/1‑140 (MQ=255)                                                                                                                      cACGCGTCTGGTCGTCGGTACCGTTGTCACGAATGGTATTTGCCGCGAAGGTTTTCAGGCCGGAGAAGCTGAAATCCAGCCCCGGACGGTCGGTCATCGGACGCGGGAAGACAAAGCGCCCGGCAGTACCCTGAgccgcc                  >  2:771893/1‑140 (MQ=255)                                                                                                                       aCGCGTCTGGTCGTCGGTACCGTTGTCACGAATGGTATTTGCCGCGAAGGTTTTCAGGCCGGAGAAGCTGAAATCCAGCCCCGGACGGTCGGTCATCGGACGCGGGAAGACAAAGCGCCCGGCAGTACCCTGAGCCGCCa                 <  1:233758/140‑1 (MQ=255)                                                                                                                        cgcgTCTGGTCGTCGGTACCGTTGTCACGAATGGTATTTGCCGCGAAGGTTTTCAGGCCGGAGAAGCTGAAATCCAGCCCCGGACGGTCGGTCATCGGACGCGGGAAGACAAAGCGCCCGGCAGTACCCTGAGCCGCCAt                >  2:769264/1‑140 (MQ=255)                                                                                                                          cgTCTGGTCGTCGGTACCGTTGTCACGAATGGTATTTGCCGCGAAGGTTTTCAGGCCGGAGAAGCTGAAATCCAGCCCCGGACGGTCGGTCATCGGACGCGGGAAGACAAAGCGCCCGGCAGTACCCTGAGCCGCCAttt              >  2:139382/1‑140 (MQ=255)                                                                                                                          cgTCTGGTCGTCGGTACCGTTGTCACGAATGGTATTTGCCGCGAAGGTTTTCAGGCCGGAGAAGCTGAAATCCAGCCCCGGACGGTCGGTCATCGGACGCGGGAAGACAAAGCGCCCGGCAGTACCCTGAGCCGCCAttt              <  1:125106/140‑1 (MQ=255)                                                                                                                          cgTCTGGTCGTCGGTACCGTTGTCACGAATGGTATTTGCCGCGAAGGTTTTCAGGCCGGAGAAGCTGAAATCCAGCCCCGGACGGTCGGTCATCGGACGCGGGAAGACAAAGCGCCCGGCAGTACCCTGAGCCGCCAttt              >  1:104495/1‑140 (MQ=255)                                                                                                                          cgTCTGGTCGTCGGTACCGTTGTCACGAATGGTATTTGCCGCGAAGGTTTTCAGGCCGGAGAAGCTGAAATCCAGCCCCGGACGGTCGGTCATCGGACGCGGGAAGACAAAGCGCCCGGCAGTACCCTGAGCCGCCAttt              >  2:1098864/1‑140 (MQ=255)                                                                                                                           gTCTGGTCGTCGGTACCGTTGTCACGAATGGTATTTGCCGCGAAGGTTTTCAGGCCGGAGAAGCTGAAATCCAGCCCCGGACGGTCGGTCATCGGACGCGGGAAGACAAAGCGCCCGGCAGTACCCTGAGCCGCCAtttt             >  1:129287/1‑140 (MQ=255)                                                                                                                            tCTGGTCGTCGGTACCGTTGTCACGAATGGTATTTGCCGCGAAGGTTTTCAGGCCGGAGAAGCTGAAATCCAGCCCCGGACGGTCGGTCATCGGACGCGGGAAGACAAAGCGCCCGGCAGTACCCTGAGCCGCCATTTTc            >  2:245993/1‑140 (MQ=255)                                                                                                                            tCTGGTCGTCGGTACCGTTGTCACGAATGGTATTTGCCGCGAAGGTTTTCAGGCCGGAGAAGCTGAAATCCAGCCCCGGACGGTCGGTCATCGGACGCGGGAAGACAAAGCGCCCGGCAGTACCCTGAGCCGCCATTTTc            >  2:1285625/1‑140 (MQ=255)                                                                                                                              tGGTCGTCGGTCCCGTTGTAACGAATGGTTTTTGCCGCGAAGGTTTTCAGGCCGGAGAAGCTGAAATCCATCCCCGGACGGTCGGTCATCGGACGGGGGAAGACAAAGCGCCCGGCGGTACCCTGAGCAGCCATTTTCgc          <  2:881153/140‑2 (MQ=255)                                                                                                                              tGGTCGTCGGTACCGTTGTCACGAATGGTATTTGCCGCGAAGGTTTTCAGGCCGGAGAAGCTGAAATCCAGCCCCGGACGGTCGGTCATCGGACGCGGGAAGACAAAGCGCCCGGCAGTACCCTGAGCCGCCATTTTCGa          <  2:120394/140‑1 (MQ=255)                                                                                                                                     cgGTACCGTTGTCACGAATGGTATTTGCCGCGAAGGTTTTCAGGCCGGAGAAGCTGAAATCCAGCCCCGGACGGTCGGTCATCGGACGCGGGAAGACAAAGCGCCCGGCAGTACCCTGAGCCGCCATTTTCGACAGTAAc   >  1:382514/1‑140 (MQ=255)                                                                                                                                      ggTACCGTTGTCACGAATGGTATTTGCCGCGAAGGTTTTCAGGCCGGAGAAGCTGAAATCCAGCCCCGGACGGTCGGTCATCGGACGCGGGAAGACAAAGCGCCCGGCAGTACCCTGAGCCGCCATTTTCGACAGTAACg  <  2:184535/140‑1 (MQ=255)                                                                                                                                         |                                                                                                                                         TAGCACTCACGCCGCCCGCCATGACCAGTCGCTTAAAGCCCGTCTGATCCAGCGCCCGCTTGCACTTAATCATCAGCGTATCGACCACCGCATCTTCAAAGGCGCGGGCGATATCAGCACGCGTCTGGTCGTCGGTGCCGTTGTCACGAATGGTATTTGCCGCGAAGGTTTTCAGGCCGGAGAAGCTGAAATCCAGCCCCGGACGGTCGGTCATCGGACGCGGGAAGACAAAGCGCCCGGCAGTACCCTGAGCCGCCATTTTCGACAGTAACG  >  NC_000913/3209730‑3210002

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑