Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,222,236	C→T	G257G (GGC→GGT)	ebgR →	DNA‑binding transcriptional repressor EbgR

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,222,236	0	C	T	100.0%	196.1 / NA	57	G257G (GGC→GGT)	ebgR	DNA‑binding transcriptional repressor EbgR
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (27/30);  total (27/30)

GCGCGGCGGTTTTTCCAGTTCGTCGGGTTATGAACTGGCAAAACAAATGCTGGCGCGGGAAGACTATCCGAAGGCACTGTTTGTTGCTTCCGATTCCATTGCTATCGGCGTACTGCGGGCAATTCATGAACGAGGCCTGAACATCCCACAGGATATTTCGCTTATCAGCGTTAACGATATCCCCACCGCGCGATTTACCTTTCCGCCGCTCTCCACCGTGCGCATCCATTCCGAGATGATGGGAAGTCAGGGCGTTAACCTGGTGTATGAAA  >  NC_000913/3222101‑3222372                                                                                                                                        |                                                                                                                                         gcgcGGCGGTTTTTCCAGTTCGTCGGGTTATGAACTGGCAAAACAAATGCTGGCGCGGGAAGACTATCCGAAGGCACTGTTTGTTGCTTCCGATTCCATTGCTATCGGCGTACTGCGGGCAATTCATGAACGAGGTCTGa                                                                                                                                      >  1:393134/1‑140 (MQ=255) gcgcGGCGGTTTTTCCAGTTCGTCGGGTTATGAACTGGCAAAACAAATGCTGGCGCGGGAAGACTATCCGAAGGCACTGTTTGTTGCTTCCGATTCCATTGCTATCGGCGTACTGCGGGCAATTCATGAACGAGGTCTGa                                                                                                                                      >  2:62111/1‑140 (MQ=255)   gcggcgGTTTTTCCAGTTCGTCGGGGTATGAACTGGCAAAACAAATGCTGGCGCGGGAAGACTATCCGAAGGCACTGTTTGTTGCTTCCGATTCCATTGCTATCGGCGTACTGCGGGCAATTCATGAACGAGGTCTGaaa                                                                                                                                    >  2:950203/1‑139 (MQ=255)    cggcggTTTTTCCAGTTCGTCGGGTTATGAACTGGCAAAACAAATGCTGGCGCGGGAAGACTATCCGAAGGCACTGTTTGTTGCTTCCGATTCCATTGCTATCGGCGTACTGCGGGCAATTCATGAACGAGGTCTGAACa                                                                                                                                   <  2:789781/140‑1 (MQ=255)     ggcggTTTTTCCAGTTCGTCGGGTTATGAACTGGCAAAACAAATGCTGGCGCGGGAAGACTATCCGAAGGCACTGTTTGTTGCTTCCGATTCCATTGCTATCGGCGTACTGCGGGCAATTCATGAACGAGGTCTGAACAt                                                                                                                                  >  1:811724/1‑140 (MQ=255)      gcggTTTTTCCAGTTCGTCGGGTTATGAACTGGCAAAACAAATGCTGGCGCGGGAAGACTATCCGAAGGCACTGTTTGTTGCTTCCGATTCCATTGCTATCGGCGTACTGCGGGCAATTCATGAACGAGGTCTGAACATc                                                                                                                                 >  2:74545/1‑140 (MQ=255)      gcggTTTTTCCAGTTCGTCGGGTTATGAACTGGCAAAACAAATGCTGGCGCGGGAAGACTATCCGAAGGCACTGTTTGTTGCTTCCGATTCCATTGCTATCGGCGTACTGCGGGCAATTCATGAACGAGGTCTGAACATc                                                                                                                                 <  1:62111/140‑1 (MQ=255)       cggTTTTTCCAGTTCGTCGGGTTATGAACTGGCAAAACAAATGCTGGCGCGGGAAGACTATCCGAAGGCACTGTTTGTTGCTTCCGATTCCATTGCTATCGGCGTACTGCGGGCAATTCATGAACGAGGTCTGAACATcc                                                                                                                                >  2:1140471/1‑140 (MQ=255)        ggTTTTTCCAGTTCGTCGGGTTATGAACTGGCAAAACAAATGCTGGCGCGGGAAGACTATCCGAAGGCACTGTTTGTTGCTTCCGATTCCATTGCTATCGGCGTACTGCGGGCAATTCATGAACGAGGTCTGAACATccc                                                                                                                               <  1:700251/140‑1 (MQ=255)         gTTTTTCCAGTTCGTCGGGTTATGAACTGGCAAAACAAATGCTGGCGCGGGAAGACTATCCGAAGGCACTGTTTGTTGCTTCCGATTCCATTGCTATCGGCGTACTGCGGGCAATTCATGAACGAGGTCTGAACATCCca                                                                                                                              <  2:1164468/140‑1 (MQ=255)                      gtcgGGTTATGAACTGGCAAAACAAATGCTGGCGCGGGAAGACTATCCGAAGGCACTGTTTGTTGCTTCCGATTCCATTGCTATCGGCGTACTGCGGGCAATTCATGAACGAGGTCTGAACATCCCACAGGATATTTCGc                                                                                                                 >  2:439926/1‑140 (MQ=255)                        cgGGTTATGAACTGGCAAAACAAATGCTGGCGCGGGAAGACTATCCGAAGGCACTGTTTGTTGCTTCCGATTCCATTGCTATCGGCGTACTGCGGGCAATTCATGAACGAGGTCTGAACATCCCACAGGATATTTCGCtt                                                                                                               >  1:732580/1‑140 (MQ=255)                           gTTATGAACTGGCAAAACAAATGCTGGCGCGGGAAGACTATCCGAAGGCACTGTTTGTTGCTTCCGATTCCATTGCTATCGGCGTACTGCGGGCAATTCATGAACGAGGTCTGAACATCCCACAGGATATTTCGCTTATc                                                                                                            <  1:551053/140‑1 (MQ=255)                           gTTATGAACTGGCAAAACAAATGCTGGCGCGGGAAGACTATCCGAAGGCACTGTTTGTTGCTTCCGATTCCATTGCTATCGGCGTACTGCGGGCAATTCATGAACGAGGTCTGAACATCCCACAGGATATTTCGCTTATc                                                                                                            <  1:37283/140‑1 (MQ=255)                                gAACTGGCAAAACAAATGCTGGCGCGGGAAGACTATCCGAAGGCACTGTTTGTTGCTTCCGATTCCATTGCTATCGGCGTACTGCGGGCAATTCATGAACGAGGTCTGAACATCCCACAGGATATTTCGCTTATCAGCGt                                                                                                       <  1:636649/140‑1 (MQ=255)                                 aaCTGGCAAAACAAATGCTGGCGCGGGAAGACTATCCGAAGGCACTGTTTGTTGCTTCCGATTCCATTGCTATCGGCGTACTGCGGGCAATTCATGAACGAGGTCTGAACATCCCACAGGATATTTCGCTTATCAGCGtt                                                                                                      <  1:371950/140‑1 (MQ=255)                                    tGGCAAAACAAATGCTGGCGCGGGAAGACTATCCGAAGGCACTGTTTGTTGCTTCCGATTCCATTGCTATCGGCGTACTGCGGGCAATTCATGAACGAGGTCTGAACATCCCACAGGATATTTCGCTTATCAGCGTTAAc                                                                                                   <  1:74545/140‑1 (MQ=255)                                    tGGCAAAACAAATGCTGGCGCGGGAAGACTATCCGAAGGCACTGTTTGTTGCTTCCGATTCCATTGCTATCGGCGTACTGCGGGCAATTCATGAACGAGGTCTGAACATCCCACAGGATATTTCGCTTATCAGCGTTAAc                                                                                                   <  1:1001957/140‑1 (MQ=255)                                     ggCAAAACAAATGCTGGCGCGGGAAGACTATCCGAAGGCACTGTTTGTTGCTTCCGATTCCATTGCTATCGGCGTACTGCGGGCAATTCATGAACGAGGTCTGAACATCCCACAGGATATTTCGCTTATCAGCGTTAACg                                                                                                  >  1:526714/1‑140 (MQ=255)                                              aaTGCTGGCGCGGGAAGACTATCCGAAGGCACTGTTTGTTGCTTCCGATTCCATTGCTATCGGCGTACTGCGGGCAATTCATGAACGAGGTCTGAACATCCCACAGGATATTTCGCTTATCAGCGTTAACGATATCCCCa                                                                                         <  1:68185/140‑1 (MQ=255)                                                tGCTGGCGCGGGAAGACTATCCGAAGGCACTGTTTGTTGCTTCCGATTCCATTGCTATCGGCGTACTGCGGGCAATTCATGAACGAGGTCTGAACATCCCGCAGGATATTTCGCTTATCAGCGTTAACGATATCCCCAcc                                                                                       >  2:904374/1‑140 (MQ=255)                                                tGCTGGCGCGGGAAGACTATCCGAAGGCACTGTTTGTTGCTTCCGATTCCATTGCTATCGGCGTACTGCGGGCAATTCATGAACGAGGTCTGAACATCCCACAGGATATTTCGCTTATCAGCGTTAACGATATCCCCAcc                                                                                       >  1:537186/1‑140 (MQ=255)                                                 gCTGGCGCGGGAAGACTATCCGAAGGCACTGTTTGTTGCTTCCGATTCCATTGCTATCGGCGTACTGCGGGCAATTCATGAACGAGGTCTGAACATCCCACAGGATATTTCGCTTATCAGCGTTAACGATATCCCCACcg                                                                                      <  2:565765/140‑1 (MQ=255)                                                 gCTGGCGCGGGAAGACTATCCGAAGGCACTGTTTGTTGCTTCCGATTCCATTGCTATCGGCGTACTGCGGGCAATTCATGAACGAGGTCTGAACATCCCACAGGATATTTCGCTTATCAGCGTTAACGATATCCCCACcg                                                                                      <  2:732580/140‑1 (MQ=255)                                                       gcgGGAAGACTATCCGAAGGCACTGTTTGTTGCTTCCGATTCCATTGCTATCGGCGTACTGCGGGCAATTCATGAACGAGGTCTGAACATCCCACAGGATATTTCGCTTATCAGCGTTAACGATATCCCCACCGCGCGAt                                                                                >  2:408688/1‑140 (MQ=255)                                                                  aTCCGAAGGCACTGTTTGTTGCTTCCGATTCCATTGCTATCGGCGTACTGCGGGCAATTCATGAACGAGGTCTGAACATCCCACAGGATATTTCGCTTATCAGCGTTAACGATATCCCCACCGCGCGATTTACCTTTccg                                                                     <  1:1296484/140‑1 (MQ=255)                                                                   tCCGAAGGCACTGTTTGTTGCTTCCGATTCCATTGCTATCGGCGTACTGCGGGCAATTCATGAACGAGGTCTGAACATCCCACAGGATATTTCGCTTATCAGCGTTAACGATATCCCCACcgcg                                                                                    <  2:388596/124‑1 (MQ=255)                                                                   tCCGAAGGCACTGTTTGTTGCTTCCGATTCCATTGCTATCGGCGTACTGCGGGCAATTCATGAACGAGGTCTGAACATCCCACAGGATATTTCGCTTATCAGCGTTAACGATATCCCCACcgcg                                                                                    >  1:388596/1‑124 (MQ=255)                                                                    ccGAAGGCACTGTTTGTTGCTTCCGATTCCATTGCTATCGGCGTACTGCGGGCAATTCATGAACGAGGTCTGAACATCCCACAGGATATTTCGCTTATCAGTGTTAACGATATCCCCACCGCGCGATTTACCTTTccgcc                                                                   <  1:865366/140‑1 (MQ=255)                                                                           cACTGTTTGTTGCTTCCGATTCCATTGCTATCGGCGTACTGCGGGCAATTCATGAACGAGGTCTGAACATCCCACAGGATATTTCGCTTATCAGCGTTAACGATATCCCCACCGCGCGATTTACCTTTCCGCCGCTCTcc                                                            <  2:691386/140‑1 (MQ=255)                                                                             cTGTTTGTTGCTTCCGATTCCATTGCTATCGGCGTACTGCGGGCAATTCATGAACGAGGTCTGAACATCCCACAGGATATTTCGCTTATCAGCGTTAACGATATCCCCACCGCGCGATTTACCTTTCCGCCGCTCTCCAc                                                          <  2:526714/140‑1 (MQ=255)                                                                                     tgCTTCCGATTCCATTGCTATCGGCGTACTGCGGGCAATTCATGAACGAGGTCTGAACATCCCACAGGATATTTCGCTTATCAGCGTTAACGATATCCCCACCGCGCGATTTACCTTTCCTCCGCTCTCCACCGTGCGCa                                                  >  1:406452/1‑140 (MQ=255)                                                                                      gCTTCCGATTCCATTGCTATCGGCGTACTGCGGGCAATTCATGAACGAGGTCTGAACATCCCACAGGATATTTCGCTTATCAGCGTTAACGATATCCCCACCGCGCGATTTACCTTTCCGCCGCTCTCCACCGTGCGCAt                                                 <  2:537186/140‑1 (MQ=255)                                                                                          ccGATTCCATTGCTATCGGCGTACTGCGGGCAATTCATGAACGAGGTCTGAACATCCCACAGGATATTTCGCTTATCAGCGTTAACGATATCCCCACCGCGCGATTTACCTTTCCGCCGCTCTCCACCGTGCGCATCCAt                                             >  2:1030816/1‑140 (MQ=255)                                                                                          ccGATTCCATTGCTATCGGCGTACTGCGGGCAATTCATGAACGAGGTCTGAACATCCCACAGGATATTTCGCTTATCAGCGTTAACGATATCCCCACCGCGCGATTTACCTTTCCGCCGCTCTCCACCGTGCGCATCCAt                                             >  2:467586/1‑140 (MQ=255)                                                                                             aTTCCATTGCTATCGGCGTACTGCGGGCAATTCATGAACGAGGTCTGAACATCCCACAGGATATTTCGCTTATCAGCGTTAACGATATCCCCACCGCGCGATTTACCTTTCCGCCGCTCTCCACCGTGCGCATCCATTcc                                          <  1:1140471/140‑1 (MQ=255)                                                                                                   ttGCTATCGGCGTACTGCGGGCAATTCATGATCGAGGTCTGAACATCCCACAGGATATTTCGCTTATCAGCGTTAACGATATCCCCACCGCGCGATTTACCTTTCCGCCGCTCTCCACCGTGCGCATCCATTCCGAgatg                                    >  1:616569/1‑140 (MQ=255)                                                                                                    tGCTATCGGCGTACTGCGGGCAATTCATGAACGAGGTCTGAACATCCCACAGGATATTTCGCTTATCAGCGTTAACGATATCCCCACCGCGCGATTTACCTTTCCGCCGCTCTCCACCGTGCGCATCCATTCCGAgatga                                   >  1:529099/1‑140 (MQ=255)                                                                                                        aTCGGCGTACTGCGGGCAATTCATGAACGAGGTCTGAACATCCCACAGGATATTTCGCTTATCAGCGTTAACGATATCCCCACCGCGCGATTTACCTTTCCGCCGCTCTCCACCGTGCGCATCCATTCCGAGATGATggg                               >  2:1139114/1‑140 (MQ=255)                                                                                                           ggCGTACTGCGGGCAATTCATGAACGAGGTCTGAACATCCCACAGGATATTTCGCTTATCAGCGTTAACGATATCCCCACCGCGCGATTTACCTTTCCGCCGCTCTCCACCGTGCGCATCCATTCCGAGATGATGGGAAg                            >  2:756158/1‑140 (MQ=255)                                                                                                            gCGTACTGCGGGCAATTCATGAACGAGGTCTGAACATCCCACAGGATATTTCGCTTATCAGCGTTAACGATATCCCCACCGCGCGATTTACCTTTCCGCCGCTCTCCACCGTGCGCATCCATTCCGAGATGATGGGAAGt                           >  2:1190553/1‑140 (MQ=255)                                                                                                               tACTGCGGGCAATTCATGAACGAGGTCTGAACATCCCACAGGATATTTCGCTTATCAGCGTTAACGATATCCCCACCGCGCGATTTACCTTTCCGCCGCTCTCCACCGTGCGCATCCATTCCGAGATGATGGGAAGTCAg                        <  1:439926/140‑1 (MQ=255)                                                                                                                 cTGCGGGCAATTCATGAACGAGGTCTGAACATCCCACAGGATATTTCGCTTATCAGCGTTAACGATATCCCCACCGCGCGATTTACCTTTCCGCCGCTCTCCACCGTGCGCATCCATTCCGAGATGATGGGAAGTCAggg                      >  1:245191/1‑140 (MQ=255)                                                                                                                   gCGGGCAATTCATGATCGAGGTCTGAACATCCCACAGGATATTTCGCTTATCAGCGTTAACGATATCCCCACCGCGCGATTTACCTTTCCGCCGCTCTCCACCGTGCGCATCCATTCCGAGATGATGGGAAGTCAGGGCg                    <  2:616569/140‑1 (MQ=255)                                                                                                                   gCGGGCAATTCATGAACGAGGTCTGAACATCCCACAGGATATTTCGCTTATCAGCGTTAACGATATCCCCACCGCGCGATTTACCTTTCCGCCGCTCTCCACCGTGCGCATCCATTCCGAGATGATGGGAAGTCAGGGCg                    >  2:634300/1‑140 (MQ=255)                                                                                                                    cGGGCAATTCATGAACGAGGTCTGAACATCCCACAGGATATTTCGCTTATCAGCGTTAACGATATCCCCACCGCGCGATTTACCTTTCCGCCGCTCTCCACCGTGCGCATCCATTCCGAGATGATGGGAAGTCAGGGCGt                   <  1:467586/140‑1 (MQ=255)                                                                                                                       gCAATTCATGAACGAGGTCTGAACATCCCACAGGATATTTCGCTTATCAGCGTTAACGATATCCCCACCGCGCGATTTACCTTTCCGCCGCTCTCCACCGTGCGCATCCATTCCGAGATGATGGGAAGTCAGGGCGTTaa                >  1:170376/1‑140 (MQ=255)                                                                                                                       gCAATTCATGAACGAGGTCTGAACATCCCACAGGATATTTCGCTTATCAGCGTTAACGATATCCCCACCGCGCGATTTACCTTTCCGCCGCTCTCCACCGTGCGCATCCATTCCGAGATGATGGGAAGTCAGGGCGTTaa                <  1:756158/140‑1 (MQ=255)                                                                                                                         aaTTCATGAACGAGGTCTGAACATCCCACAGGATATTTCGCTTATCAGCGTTAACGATATCCCCACCGCGCGATTTACCTTTCCGCCGCTCTCCACCGTGCGCATCCATTCCGAGATGATGGGAAGTCAGGGCGTTAAcc              <  2:245191/140‑1 (MQ=255)                                                                                                                         aaTTCATGAACGAGGTCTGAACATCCCACAGGATATTTCGCTTATCAGCGTTAACGATATCCCCACCGCGCGATTTACCTTTCCGCCGCTCTCCACCGTGCGCATCCATTCCGAGATGATGGGAAGTCAGGGCGTTAAcc              >  1:769260/1‑140 (MQ=255)                                                                                                                           ttCATGAACGAGGTCTGAACATCCCGCAGGATATTTCGCTTATCAGCGTTAACGATATCCCCACCGCGCGATTTACCTTTCCGCCGCTCTCCACCGTGCGCATCCATTCCGAGATGATGGGAAGTCAGGGCGTTAACCTg            <  1:904374/140‑1 (MQ=255)                                                                                                                           ttCATGAACGAGGTCTGAACATCCCACAGGATATTTCGCTTATCAGCGTTAACGATATCCCCACCGCGCGATTTACCTTTCCGCCGCTCTCCACCGTGCGCATCCATTCCGAGATGATGGGAAGTCAGGGCGTTAACCTg            <  2:393134/140‑1 (MQ=255)                                                                                                                           ttCATGAACGAGGTCTGAACATCCCACAGGATATTTCGCTTATCAGCGTTAACGATATCCCCACCGCGCGATTTACCTTTCCGCCGCTCTCCACCGTGCGCATCCATTCCGAGATGATGGGAAGTCAGGGCGTTAACCTg            <  1:1030816/140‑1 (MQ=255)                                                                                                                                   cGAGGTCTGAACATCCCACAGGATATTTCGCTTATCAGCGTTAACGATATCCCCACCGCGCGATTTACCTTTCCGCCGCTCTCCACCGTGCGCATCCATTCCGAGATGATGGGAAGTCAGGGCGTTAAcc              >  2:825987/1‑130 (MQ=255)                                                                                                                                   cGAGGTCTGAACATCCCACAGGATATTTCGCTTATCAGCGTTAACGATATCCCCACCGCGCGATTTACCTTTCCGCCGCTCTCCACCGTGCGCATCCATTCCGAGATGATGGGAAGTCAGGGCGTTAAcc              <  1:825987/130‑1 (MQ=255)                                                                                                                                     aGGTCTGAACATCCCACAGGATATTTCGCTTATCAGCGTTAACGATATCCCCACCGCGCGATTTACCTTTCCGCCGCTCTCCACCGTGCGCATCCATTCCGAGATGATGGGAAGTCAGGGCGTTAAcc              >  2:1177552/1‑128 (MQ=255)                                                                                                                                     aGGTCTGAACATCCCACAGGATATTTCGCTTATCAGCGTTAACGATATCCCCACCGCGCGATTTACCTTTCCGCCGCTCTCCACCGTGCGCATCCATTCCGAGATGATGGGAAGTCAGGGCGTTAAcc              <  1:1177552/128‑1 (MQ=255)                                                                                                                                     aGGTCTGAACATCCCACAGGATATTTCGCTTATCAGCGTTAACGATATCCCCACCGCGCGATTTACCTTTCCGCCGCTCTCCACCGTGCGCATCCATTCCGAGATGATGGGAAGTCAGGGCGTTAACCTGGTGTATGaaa  >  2:192294/1‑140 (MQ=255)                                                                                                                                        |                                                                                                                                         GCGCGGCGGTTTTTCCAGTTCGTCGGGTTATGAACTGGCAAAACAAATGCTGGCGCGGGAAGACTATCCGAAGGCACTGTTTGTTGCTTCCGATTCCATTGCTATCGGCGTACTGCGGGCAATTCATGAACGAGGCCTGAACATCCCACAGGATATTTCGCTTATCAGCGTTAACGATATCCCCACCGCGCGATTTACCTTTCCGCCGCTCTCCACCGTGCGCATCCATTCCGAGATGATGGGAAGTCAGGGCGTTAACCTGGTGTATGAAA  >  NC_000913/3222101‑3222372

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑