Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,231,341	A→G	intergenic (+102/‑324)	ygjK → / → fadH	glycoside hydrolase/2,4‑dienoyl‑CoA reductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,231,341	0	A	G	100.0%	237.8 / NA	66	intergenic (+102/‑324)	ygjK/fadH	glycoside hydrolase/2,4‑dienoyl‑CoA reductase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (30/36);  total (30/36)

ATGTTGTATAACGATTTTTTCCGTAAGCAATAATTTGCAACTGCTGGCGGATGCGGCGTAAACGCCTTATCCGCCCTACATGTGCAATCCTCCTGGCCGGATAAAACGCGGCAAGCGTTGCATCCGGCAACTGCACCGCGCCACTGGCGGATGCGGCGTGAACGCCTTATCCGCCCTACATGTGTGTTCCCGTAGGTCGGATAAGACGCGACAAGCGTCGCATCCGGCATCTGCACCGCGCCACTGGCGGATGCGGCGTGGACGCCTTATC  >  NC_000913/3231207‑3231477                                                                                                                                       |                                                                                                                                         atGTTGTATAACGATTTTTTCCGTAAGCAATAATTTGCAACTGCTGGCGGATGCGGCGTAAACGCCTTATCCGCCCTACATGTGCAATCCTCCTGGCCGGATAAAACGCGGCAAGCGTTGCATCCGGCAACTGCGCcgcg                                                                                                                                     <  2:406100/140‑1 (MQ=255)   gttgtATAACGATTTTTTCCGTAAGCAATAATTTGCAACTGCTGGCGGATGCGGCGTAAACGCCTTATCCGCCCTACATGTGCAATCCTCCTGGCCGGATAAAACGCGGCAAGCGTTGCATCCGGCAACTgcgccgcgcc                                                                                                                                   <  2:879054/140‑1 (MQ=255)     tgtATAACGATTTTTTCCGTAAGCAATAATTTGCAACTGCTGGCGGATGCGGCGTAAACGCCTTATCCGCCCTACATGTGCAATCCTCCTGGCCGGATAAAACGCGGCAAGCGTTGCATCCGGCAACTGCGCCGCGCCa                                                                                                                                  <  2:266072/139‑1 (MQ=255)     tgtATAACGATTTTTTCCGTAAGCAATAATTTGCAACTGCTGGCGGATGCGGCGTAAACGCCTTATCCGCCCTACATGTGCAATCCTCCTGGCCGGATAAAACGCGGCAAGCGTTGCATCCGGCAACTGCGCCGCGCCa                                                                                                                                  >  1:266072/1‑139 (MQ=255)     tgtATAACGATTTTTTCCGTAAGCAATAATTTGCAACTGCTGGCGGATGCGGCGTAAACGCCTTATCCGCCCTACATGTGCAATCCTCCTGGCCGGATAAAACGCGGCAAGCGTTGCATCCGGCAACTGCGCCGCGCCAc                                                                                                                                 >  1:1204483/1‑140 (MQ=255)     tgtATAACGATTTTTTCCGTAAGCAATAATTTGCAACTGCTGGCGGATGCGGCGTAAACGCCTTATCCGCCCTACATGTGCAATCCTCCTGGCCGGATAAAACGCGGCAAGCGTTGCATCCGGCAACTGCGCCGCGCCAc                                                                                                                                 >  2:482946/1‑140 (MQ=255)     tgtATAACGATTTTTTCCGTAAGCAATAATTTGCAACTGCTGGCGGATGCGGCGTAAACGCCTTATCCGCCCTACATGTGCAATCCTCCTGGCCGGATAAAACGCGGCAAGCGTTGCATCCGGCAACTGCGCCGCGCCAc                                                                                                                                 >  2:600023/1‑140 (MQ=255)     tgtATAACGATTTTTTCCGTAAGCAATAATTTGCAACTGCTGGCGGATGCGGCGTAAACGCCTTATCCGCCCTACATGTGCAATCCTCCTGGCCGGATAAAACGCGGCAAGCGTTGCATCCGGCAACTGCGCCGCGCCAc                                                                                                                                 >  1:987431/1‑140 (MQ=255)     tgtATAACGATTTTTTCCGTAAGCAATAATTTGCAACTGCTGGCGGATGCGGCGTAAACGCCTTATCCGCCCTACATGTGCAATCCTCCTGGCCGGATAAAACGCGGCAAGCGTTGCATCCGACAACTGCGCCGCGCCAc                                                                                                                                 >  2:781581/1‑140 (MQ=255)       tataACGATTTTTTCCGTAAGCAATAATTTGCAACTGCTGGCGGATGCGGCGTAAACGCCTTATCCGCCCTACATGTGCAATCCTCCTGGCCGGATAAAACGCGGCAAGCGTTGCATCCGGCAACTGCGCCGCGCCACTg                                                                                                                               >  1:355627/1‑140 (MQ=255)       tataACGATTTTTTCCGTAAGCAATAATTTGCAACTGCTGGCGGATGCGGCGTAAACGCCTTATCCGCCCTACATGTGCAATCCTCCTGGCCGGATAAAACGCGGCAAGCGTTGCATCCGGCAACTGCGCCGCGCCACTg                                                                                                                               >  1:337675/1‑140 (MQ=255)        ataACGATTTTTTCCGTAAGCAATAATTTGCAACTGCTGGCGGATGCGGCGTAAACGCCTTATCCGCCCTACATGTGCAATCCTCCTGGCCGGATAAAATGCGGCAAGCGTTGCATCCGGCAACTGCGCCGCGCCACTgg                                                                                                                              >  2:1304621/1‑140 (MQ=255)        ataACGATTTTTTCCGTAAGCAATAATTTGCAACTGCTGGCGGATGCGGCGTAAACGCCTTATCCGCCCTACATGTGCAATCCTCCTGGCCGGATAAAACGCGGCAAGCGTTCCATCCGGCAACTgcgccgcgcc                                                                                                                                   >  1:777672/1‑135 (MQ=255)        ataACGATTTTTTCCGTAAGCAATAATTTGCAACTGCTGGCGGATGCGGCGTAAACGCCTTATCCGCCCTACATGTGCAATCCTCCTGGCCGGATAAAACGCGGCAAGCGTTCCATCCGGCAACTgcgccgcgcc                                                                                                                                   <  2:777672/135‑1 (MQ=255)         taACGATTTTTTCCGTAAGCAATAATTTGCAACTGCTGGCGGATGCGGCGTAAACGCCTTATCCGCCCTACATGTGCAATCCTCCTGGCCGGATAAAACGCGGCAAGCGTTGCATCCGGCAACTGCGCCGCGCCACTGGc                                                                                                                             >  1:1369372/1‑140 (MQ=255)             gATTTTTTCCGTAAGCAATAATTTGCAACTGCTGGCGGATGCGGCGTAAACGCCTTATCCGCCCTACATGTGCAATCCTCCTGGCCGGATAAAACGCGGCAAGCGTTGCATCCGGCAACTGCGCCGCGCCACTGGCGGAt                                                                                                                         <  1:1222129/140‑1 (MQ=255)              aTTTTTTCCGTAAGCAATAATTTGCAACTGCTGGCGGATGCGGCGTAAACGCCTTATCCGCCCTACATGTGCAATCCTCCTGGCCGGATAAAACGCGGCAAGCGTTGCATCCGGCAACTGCGCCGCGCCACTGGCGGATg                                                                                                                        >  1:167125/1‑140 (MQ=255)                 ttttCCGTAAGCAATAATTTGCAACTGCTGGCGGATGCGGCGTAAACGCCTTATCCGCCCTACATGTGCAATCCTCCTGGCCGGATAAAACGCGGCAAGCGTTGCATCCGGCAACTGCGCCGCGCCACTGGCGGATgcgg                                                                                                                     >  1:16193/1‑140 (MQ=255)                     ccGTAAGCAATAATTTGCAACTGCTGGCGGATGCGGCGTAAACGCCTTATCCGCCCTACATGTGCAATCCTCCTGGCCGGATAAAACGCGGCAAGCGTTGCATCCGGCAACTGCGCCGCGCCACTGGCGGATGCGGCGTg                                                                                                                 <  1:700623/140‑1 (MQ=255)                     ccGTAAGCAATAATTTGCAACTGCTGGCGGATGCGGCGTAAACGCCTTATCCGCCCTACATGTGCAATCCTCCTGGCCGGATAAAACGCGGCAAGCGTTGCATCCGGCAACTGCGCCGCGCCACTGGCGGATGCGGCGTg                                                                                                                 <  1:701247/140‑1 (MQ=255)                         aaGCAATAATTTGCAACTGCTGGCGGATGCGGCGTAAACGCCTTATCCGCCCTACATGTGCAATCCTCCTGGCCGGATAAAACGCGGCAAGCGTTGCATCCGGCAACTGCGCCGCGCCACTGGCGGATGCGGCGTGAACg                                                                                                             >  2:344096/1‑140 (MQ=255)                         aaGCAATAATTTGCAACTGCTGGCAGATGCGGCGTAAACGCCTTATCCGCCCTACATGTGCAATCCTCCTGGCCGGATAAAACGCGGCAAGCGTTGCATCCGGCAACTGCGCCGCGCCACTGGCGGATGCGGCGtataca                                                                                                             >  2:875119/1‑135 (MQ=255)                              ataatTTGCAACTGCTGGCGGATGCGGCGTAAACGCCTTATCCGCCCTACATGTGCAATCCTCCTGGCCGGATAAAACGCGGCAAGCGTTGCATCCGGCAACTGCGCCGCGCCACTGGCGGATGCGGCGTGAACGCCTTa                                                                                                        <  1:875119/140‑1 (MQ=255)                               taatTTGCAACTGCTGGCGGATGCGGCGTAAACGCCTTATCCGCCCTACATGTGCAATCCTCCTGGCCGGATAAAACGCGGCAAGCGTTGCATCCGGCAACTGCGCCGCGCCACTGGCGGATGCGGCGTGAACGCCTTAt                                                                                                       <  2:1204483/140‑1 (MQ=255)                               taatTTGCAACTGCTGGCGGATGCGGCGTAAACGCCTTATCCGCCCTACATGTGCAATCCTCCTGGCCGGATAAAACGCGGCAAGCGTTGCATCCGGCAACTGCGCCGCGCCACTGGCGGATGCGGCGTGAACGCCTTAt                                                                                                       <  2:370431/140‑1 (MQ=255)                                aatTTGCAACTGCTGGCGGATGCGGCGTAAACGCCTTATCCGCCCTACATGTGCAATCCTCCTGGCCGGATAAAACGCGGCAAGCGTTGCATCCGGCAACTGCGCCGCGCCACTGGCGGATGCGGCGTGAACGCCTTATc                                                                                                      <  2:16193/140‑1 (MQ=255)                                aatTTGCAACTGCTGGCGGATGCGGCGTAAACGCCTTATCCGCCCTACATGTGCAATCCTCCTGGCCGGATAAAACGCGGCAAGCGTTGCATCCGGCAACTGCGCCGCGCCACTGGCGGATGCGGCGTGAACGCCTTATc                                                                                                      <  2:530379/140‑1 (MQ=255)                                aatTTGCAACTGCTGGCGGATGCGGCGTAAACGCCTTATCCGCCCTACATGTGCAACCCTCCTGGCCGGATAAAACGCGGCAAGTGTTGCATCGGGCAACTGCGCCCCGCCACTGGCGGATGCGGCGTGAACGCCTTATc                                                                                                      <  2:164834/140‑1 (MQ=18)                                  tttGTAACTGCTGGCGGATGCGGCGTAAACGCCTTATCCGCCCTACATGTGCAATCCTCCTGGCCGGATAAAACGCGGCAAGCGTTGCATCCGGCAACTGCGCCGCGCCACTGGCGGATGCGGCGTGAACGCCTTATCCg                                                                                                    <  2:1308079/140‑1 (MQ=255)                                   ttGCAACTGCTGGCGGATGCGGCGTAAACGCCTTATCCGCCCTACATGTGCAATCCTCCTGGCCGGATAAAACGCGGCAAGCGTTGCATCCGGCAACTGCGCCGCGCCACTGGCGGATGCGGCGTGAACGCCTTATCCGc                                                                                                   <  1:983678/140‑1 (MQ=255)                                    tGCAACTGCTGGCGGATGCGGCGTAAACGCCTTATCCGCCCTACATGTGCAATCCTCCTGGCCGGATAAAACGCGGCAAGCGTTGCATCCGGCAACTGCGCCGCGCCACTGGCGGATGCGGCGTGAACGCCTTATCCGcc                                                                                                  <  2:337675/140‑1 (MQ=255)                                    tGCAACTGCTGGCGGATGCGGCGTAAACGCCTTATCCGCCCTACATGTGCAATCCTCCTGGCCGGATAAAACGCGGCAAGCGTTGCATCCGGCAACTGCGCCGCGCCACTGGCGGATGCGGCGTGAACGCCTTATCCGcc                                                                                                  <  1:333594/140‑1 (MQ=255)                                     gCAACTGCTGGCGGATGCGGCGTAAACGCCTTATCCGCCCTACATGTGCAATCCTCCTGGCCGGATAAAATGCGGCAAGCGTTGCATCCGGCAACTGCGCCGCGCCACTGGCGGATGCGGCGTGAACGCCTTATCCGccc                                                                                                 <  1:1304621/140‑1 (MQ=255)                                     gCAACTGCTGGCGGATGCGGCGTAAACGCCTTATCCGCCCTACATGTGCAATCCTCCTGGCCGGATAAAACGCGGCAAGCGTTGCATCCGGCAACTGCGCCGCGCCACTGGCGGATGCGGCGTGAACGCCTTATCCGccc                                                                                                 >  1:941074/1‑140 (MQ=255)                                     gCAACTGCTGGCGGATGCGGCGTAAACGCCTTATCCGCCCTACATGTGCAATCCTCCTGGCCGGATAAAACGCGGCAAGCGTTGCATCCGGCAACTGCGCCGCGCCACTGGCGGATGCGGCGTGAACGCCTTATCCGccc                                                                                                 <  1:344096/140‑1 (MQ=255)                                     gCAACTGCTGGCGGATGCGGCGTAAACGCCTTATCCGCCCTACATGTGCAATCCTCCTGGCCGGATAAAACGCGGCAAGCGTTGCATCCGGCAACTGCGCCGCGCCACTGGCGGATGCGGCGTGAACGCCTTATCCGccc                                                                                                 >  1:1248292/1‑140 (MQ=255)                                           gctgGCGGATGCGGCGTAAACGCCTTATCCGCCCTACATGTGCAATCCTCCTGGCCGGATAAAACGCGGCAAGCGTTGCATCCGGCAACTGCGCCGCGCCACTGGCGGATGCGGCGGGGACGCCTTTTCCGCCCCAcctt                                                                                           >  2:1289279/1‑137 (MQ=17)                                            ctgGCGGATGCGGCGTAAACGCCTTATCCGCCCTACATGTGCAATCCTCCTGGCCGGATAAAACGCGGCAAGCGTTGCATCCGGCAACTGCGCCGCGCCACTGGCGGATGCGGCGTGAACGCCTTATCCGCCCTa                                                                                               <  2:594661/135‑1 (MQ=255)                                            ctgGCGGATGCGGCGTAAACGCCTTATCCGCCCTACATGTGCAATCCTCCTGGCCGGATAAAACGCGGCAAGCGTTGCATCCGGCAACTGCGCCGCGCCACTGGCGGATGCGGCGTGAACGCCTTATCCGCCCTa                                                                                               >  1:594661/1‑135 (MQ=255)                                               gCGGATGCGGCGTAAACGCCTTATCCGCCCTACATGTGCAATCCTCCTGGCCGGATAAAACGCGGCAAGCGTTGCATCCGGCAACTGCGCCGCGCCACTGGCGGATGCGGCGTGAACGCCTTATCCGCCCTACAtgtgtg                                                                                       <  1:482946/140‑1 (MQ=255)                                                      cggcgTAAACGCCTTATCCGCCCTACATGTGCAATCCTCCTGGCCGGATAAAACGCGGCAAGCGTTGCATCCGGCAACTGCGCCGCGCCACTGGCGGATGCGGCGTGAACGCCTTATCCGCCCTACATGTGTGTTCCCGt                                                                                <  2:1244696/140‑1 (MQ=255)                                                             aaCGCCTTATCCGCCCTACATGTGCAATCCTCCTGGCCGGATAAAACGCGGCAAGCGTTGCATCCGGCAACTGCGCCGCGCCACTGGCGGATGCGGCGTGAACGCCTTATCCGCCCTACATGTGTGTTCCCGTAGGTCgg                                                                         >  2:226203/1‑140 (MQ=255)                                                                   ttATCCGCCCTACATGTGCAATCCTCCTGGCCGGATAAAACGCGGCAAGCGTTGCATCCGGCAACTGCGCCGCGCCACTGGCGGATGCGGCGTGAACGCCTTATCCGCCCTACATGTGTGTTCCCGTAGGTCGGATAAGa                                                                   >  2:1310388/1‑140 (MQ=255)                                                                               cATGTGCAATCCTCCTGGCCGGATAAAACGCGGCAAGCGTTGCATCCGGCAACTGCGCCGCGCCACTGGCGGATGCGGCGTGAACGCCTTATCCGCCCTACATGTGTGTTCCCGTAGGTCGGATAAGACGCGACAAGCGt                                                       >  1:640189/1‑140 (MQ=255)                                                                                   tgCAATCCTCCTGGCCGGATAAAACGCGGCAAGCGTTGCATCCGGCAACTGCGCCGCGCCACTGGCGGATGCGGGGTGAACGCCTTATCCGCCCTACATGTGTGTTCCCGTAGGTCGGATAAGACGCGACAAGCGTCGCa                                                   <  2:1248292/140‑1 (MQ=255)                                                                                    gCAATCCTCCTGGCCGGATAAAACGCGGCAAGCGTTGCATCCGGCAACTGCGCCGCGCCACTGGCGGATGCGGCGTGAACGCCTTATCCGCCCTACATGTGTGTTCCCGTAGGTCGGATAAGACGCGACAAGCGTCGCAt                                                  >  2:904786/1‑140 (MQ=255)                                                                                     cAATCCTCCTGGCCGGATAAAACGCGGCAAGCGTTGCATCCGGCAACTGCGCCGCGCCACTGGCGGATGCGGCGTGAACGCCTTATCCGCCCTACATGTGTGTTCCCGTAGGTCGGATAAGACGCGACAAGCGTCGCATc                                                 >  2:1037866/1‑140 (MQ=255)                                                                                      aaTCCTCCTGGCCGGATAAAACGCGGCAAGCGTTGCATCCGGCAACTGCGCCGCGCCACTGGCGGATGCGGCGTGAACGCCTTATCCGCCCTACATGTGTGTTCCCGTAGGTCGGATAAGACGCGACAAGCGTCGCATcc                                                <  2:470944/140‑1 (MQ=255)                                                                                         cttcctCGCCGGATAAAACGCGGCAAGCGTTGCATCCGGCAACTGCGCCGCGCCACTGGCGGATGCGGCGTGAACGCCTTATCCGCCCTACATGTGTGGTCCCGTAGGTCGGATAAGACGCGACAAACGTCGCATCCGGc                                             >  2:843427/3‑140 (MQ=18)                                                                                         cctcctGGCCGGATAAAACGCGGCAAGCGTTGCATCCGGCAACTGCGCCGCGCCACTGGCGGATGCGGCGTGAACGCCCTTTCCGCCCTACATGTGTGTTCCCGTGGGACCGATAAGACGCGACAAGCGTCGCATCCGGc                                             >  2:530120/1‑140 (MQ=18)                                                                                         catcctGGCCGGATAAAACGCGGCAAGCGTTGCATCCGGCAACTGCGCCGCGCCACTGGCGGATGCGGCGTGAACGCCTTATCCGCCCTACATGTGTGTTCCCGTAGGTCGGATAAGACGCGACAAGCGTCGCATCCGGc                                             >  2:278066/3‑140 (MQ=255)                                                                                             ctGGCCGGATAAAACGCGGCAAGCGTTGCATCCGGCAACTGCGCCGCGCCACTGGCGGATGCGGCGTGAACGCCTTATCCGCCCTACATGTGTGTTCCCGTAGGTCGGATAAGACGCGACAAGCGTCGCATCCGGCATCt                                         >  2:1134136/1‑140 (MQ=255)                                                                                                gCCGGATAAAACGCGGCAAGCGTTGCATCCGGCAACTGCGCCGCGCCACTGGCGGATGCGGCGTGAACGCCTTATCCGCCCTACATGTGTGTTCCCGTAGGTCGGATAAGACGCGACAAGCGTCGCATCCGGCATCTGCa                                      >  2:194949/1‑140 (MQ=255)                                                                                                 ccGGATAAAACGCGGCAAGCGTTGCATCCGGCAACTGCGCCGCGCCACTGGCGGATGCGGCGTGAACGCCTTATCCGCCCTACATGTGTGTTCCCGTAGGTCGGATAAGACGCGACAAGCGTCGCATCCGGCATCTGCAc                                     <  1:1310388/140‑1 (MQ=255)                                                                                                  cGGATAAAACGCGGCAAGCGTTGCATCCGGCAACTGCGCCGCGCCACTGGCGGATGCGGCGTGAACGCCTTATCCGCCCTACATGTGTGTTCCCGTAGGTCGGATAAGACGCGACAAGCGTCGCATCCGGCATCTGCAcc                                    >  1:660755/1‑140 (MQ=255)                                                                                                    gATAAAACGCGGCAAGCGTTGCATCCGGCAACTGCGCCGCGCCACTGGCGGATGCGGCGTGAACGCCTTATCCGCCCTACATGTGTGTTCCCGTAGGTCGGATAAGACGCGACAAGCGTCGCATCCGGCATCTGCACcgc                                  >  2:735513/1‑140 (MQ=255)                                                                                                        aaaCGCGGCAAGCGTTGCATCCGGCAACTGCGCCGCGCCACTGGCGGATGCGGCGTGAACGCCTTATCCGCCCTACATGTGTGTTCCCGTAGGTCGGATAAGACGCGACAAGCGTCGCATCCGGCATCTGCACCGCGCCa                              <  1:226203/140‑1 (MQ=255)                                                                                                          aCGCGGCAAGCGTTGCATCCGGCAACTGCGCCGCGCCACTGGCGGATGCGGCGTGAACGCCTTATCCGCCCTACATGTGTGTTCCCGTAGGTTGGATAAGACGCGACAAGCGTCGCATCCGGCATCTGCACCGCGCCACt                            >  1:485918/1‑140 (MQ=255)                                                                                                                 aaGCGTTGCATCCGGCAACTGCGCCGCGCCACTGGCGGATGCGGCGTGAACGCCTTATCCGCCCTACATGTGTGTTCCCGTAGGTCGGATAAGACGCGACAAGCGTCGCATCCGGCATCTGCACCGCGCCACTGGCGGAt                     <  2:987431/140‑1 (MQ=255)                                                                                                                   gCGTTGCATCCGGCAACTGCGCCGCGCCACTGGCGGATGCGGCGTGAACGCCTTATCCGCCCTACATGTGTGTTCCCGTAGGTCGGATAAGACGCGACAAGCGTCGCATCCGGCATCTGCACCGCGCCACTGGCGGATgc                   <  2:987311/140‑1 (MQ=255)                                                                                                                    cGTTGCATCCGGCAACTGCGCCGCGCCACTGGCGGATGCGGCGTGAACGCCTTATCCGCCCTACATGTGTGTTCCCGTAGGTCGGATAAGACGCGACAAGCGTCGCATCCGGCATCTGCACCGCGCCACTGGCGGATgcg                  <  1:600023/140‑1 (MQ=255)                                                                                                                         cATCCGGCAACTGCGCCGCGCCACTGGCGGATGCGGCGTGAACGCCTTATCCGCCCTACATGTGTGTTCCCGTAGGTCGGATAAGACGCGACAAGCGTCGCATCCGGCATCTGCACCGCGCCACTGGCGGATGCGGCGTg             <  2:640189/140‑1 (MQ=255)                                                                                                                         cATCCGGCAACTGCGCCGCGCCACTGGCGGATGCGGCGTGAACGCCTTATCCGCCCTACATGTGTGTTCCCGTAGGTCGGATAAGACGCGACAAGCGTCGCATCCGGCATCTGCACCGCGCCACTGGCGGATGCGGCGTg             <  1:278066/140‑1 (MQ=255)                                                                                                                         cATCCGGCAACTGCGCCGCGCCACTGGCGGATGCGGCGTGAACGCCTTATCCGCCCTACATGTGTGTTCCCGTAGGGCGGATAAGACGCGACAAGCGTCGCATCCGGCATCTGCACCGCGCCACTGGCGGATGCGGCGTg             <  1:843427/140‑1 (MQ=255)                                                                                                                          aTCCGGCAACTGCGCCGCGCCACTGGCGGATGCGGCGTGAACGCCTTATCCGCCCTACATGTGTGTTCCCGTAGGTCGGATAAGACGCGACAAGCGTCGCATCCGGCATCTGCACCGCGCCACTGGCGGATGCGGCGTgg            <  2:182343/140‑1 (MQ=255)                                                                                                                              ggCAACTGCGCCGCGCCACTGGCGGATGCGGCGTGAACGCCTTATCCGCCCTACATGTGTGTTCCCGTAGGTCGGATAAGACGCGACAAGCGTCGCATCCGGCATCTGCACCGCGCCACTGGCGGATGCGGCGTGGACGc        <  1:1134136/140‑1 (MQ=255)                                                                                                                              ggCAACTGCGCCGCGCCACTGGCGGATGCGGCGTGAACGCCTTATCCGCCCTACATGTGTGTTCCCGTAGGTCGGATAAGACGCGACAAGCGTCGCATCCGGCATCTGCACCGCGCCACTGGCGGATGCGGCGTGGACGc        <  1:194949/140‑1 (MQ=255)                                                                                                                              ggCAACTGCGCCGCGCCACTGGCGGATGCGGCGTGAACGCCTTATCCGCCCTACATGTGTGTTCCCGTAGGTCGGATAAGACGCGACAAGCGTCGCATCCGGCATCTGCACCGCGCCACTGGCGGATGCGGCGTGGACGc        <  2:167125/140‑1 (MQ=255)                                                                                                                                  aCTGCGCCGCGCCACTGGCGGATGCGGCGTGAACGCCTTATCCGCCCTACATGTGTGTTCCCGTAGGTCGGATAAGACGCGACAAGCGTCGCATCCGGCATCTGCACCGCGCCACTGGCGGATGCGGCGTGGACGCCTTa    <  2:1369372/140‑1 (MQ=255)                                                                                                                                    tGCGCCGCGCCACTGGCGGATGCGGCGTGAACGCCTTATCCGCCCTACATGTGTGTTCCCGTAGGTCGGATAAGACGCGACAAGCGTCGCATCCGGCATCTGCACCGCGCCACTGGCGGATGCGGCGTGGACGCCTTATc  <  1:781581/140‑1 (MQ=255)                                                                                                                                       |                                                                                                                                         ATGTTGTATAACGATTTTTTCCGTAAGCAATAATTTGCAACTGCTGGCGGATGCGGCGTAAACGCCTTATCCGCCCTACATGTGCAATCCTCCTGGCCGGATAAAACGCGGCAAGCGTTGCATCCGGCAACTGCACCGCGCCACTGGCGGATGCGGCGTGAACGCCTTATCCGCCCTACATGTGTGTTCCCGTAGGTCGGATAAGACGCGACAAGCGTCGCATCCGGCATCTGCACCGCGCCACTGGCGGATGCGGCGTGGACGCCTTATC  >  NC_000913/3231207‑3231477

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑