Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,303,077	T→C	C197R (TGC→CGC)	yhbV →	putative peptidase YhbV

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,303,077	0	T	C	100.0%	259.4 / NA	70	C197R (TGC→CGC)	yhbV	putative peptidase YhbV
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (29/41);  total (29/41)

ATCAGTGCGATGAGCTGGGCATTCGTAACCAGTTTGAAGTGGAAGTCCTGAGCTACGGTCATCTGCCGCTGGCCTACTCCGCCCGCTGCTTTACCGCGCGTTCGGAAGACCGCCCGAAAGATGAGTGTGAAACCTGCTGCATTAAGTATCCGAACGGGCGCAACGTGCTGTCGCAGGAAAACCAACAAGTGTTTGTACTCAATGGCATTCAGACCATGAGCGGCTACGTTTACAACCTCGGTAACGAGCTGGCATCCATGCAGGGCCTGGTTG  >  NC_000913/3302940‑3303212                                                                                                                                          |                                                                                                                                        aTCAGTGCGATGAGCTGGGCATTCGTAACCAGTTTGAAGTGGAAGTCCTGAGCTACGGTCATCTGCCGCTGGCCTACTCCGCCCGCTGCTTTACCGCGCGTTCGGAAGACCGCCCGAAAGATGAGTGTGAAACCtgccgc                                                                                                                                       >  1:1279132/1‑140 (MQ=255)  tCAGTGCGATGAGCTGGGCATTCGTAACCAGTTTGAAGTGGAAGTCCTGAGCTACGGTCATCTGCCGCTGGCCTACTCCGCCCGCTGCTTTACCGCGCGTTCGGAAGACCGCCCGAAAGATGAGTGTGAAACCTGCCGCa                                                                                                                                      <  2:1042059/140‑1 (MQ=255)  tCAGTGCGATGAGCTGGGCATTCGTAACCAGTTTGAAGTGGAAGTCCTGAGCTACGGTCATCTGCCGCTGGCCTACTCCGCCCGCTGCTTTACCGCGCGTTCGGAAGACCGCCCGAAAGATGAGTGTGAAACCTGCCGCa                                                                                                                                      <  1:553945/140‑1 (MQ=255)  tCAGTGCGATGAGCTGGGCATTCGTAACCAGTTTGAAGTGGAAGTCCTGAGCTACGGTCATCTGCCGCTGGCCTACTCCGCCCGCTGCTTTACCGCGCGTTCGGAAGACCGCCCGAAAGATGAGTGTGAAACCTGCCGCa                                                                                                                                      <  1:814939/140‑1 (MQ=255)    aGTGCGATGAGCTGGGCATTCGTAACCAGTTTGAAGTGGAAGTCCTGAGCTACGGTCATCTGCCGCTGGCCTACTCCGCCCGCTGCTTTACCGCGCGTTCGGAAGACCGCCCGAAAGATGAGTGTGAAACCTGCCGCAtt                                                                                                                                    <  1:816372/140‑1 (MQ=255)       gCGATGAGCTGGGCATTCGTAACCAGTTTGAAGTGGAAGTCCTGAGCTACGGTCATCTGCCGCTGGCCTACTCCGCCCGCTGCTTTACCGCGCGTTCGGAAGACCGCCCGAAAGATGAGTGTGAAACCTGCCGCATTAAg                                                                                                                                 >  2:1404222/1‑140 (MQ=255)       gCGATGAGCTGGGCATTCGTAACCAGTTTGAAGTGGAAGTCCTGAGCTACGGTCATCTGCCGCTGGCCTACTCCGCCCGCTGCTTTACCGCGCGTTCGGAAGACCGCCCGAAAGATGAGTGTGAAACCTGCCGCATTAAg                                                                                                                                 >  1:1148623/1‑140 (MQ=255)       gCGATGAGCTGGGCATTCGTAACCAGTTTGAAGTGGAAGTCCTGAGCTACGGTCATCTGCCGCTGGCCTACTCCGCCCGCTGCTTTACCGCGCGTTCGGAAGACCGCCCGAAAGATGAGTGTGAAACCTGCCGCATTAAg                                                                                                                                 >  2:1207570/1‑140 (MQ=255)             aGCTGGGCATTCGTAACCAGTTTGAAGTGGAAGTCCTGAGCTACGGTCATCTGCCGCTGGCCTACTCCGCCCGCTGCTTTACCGCGCGTTCGGAAGACCGCCCGAAAGATGAGTGTGAAACCTGCCGCATTAAGTATCCg                                                                                                                           <  2:732218/140‑1 (MQ=255)               cTGGGCATTCGTAACCAGTTTGAAGTGGAAGTCCTGAGCTACGGTCATCTGCCGCTGGCCTACTCCGCCCGCTGCTTTACCGCGCGTTCGGAAGACCGCCCGAAAGATGAGTGTGAAACCTGCCGCATTAAGTATCCGaa                                                                                                                         <  2:753327/140‑1 (MQ=255)                  ggCATTCGTAACCAGTTTGAAGTGGAAGTCCTGAGCTACGGTCATCTGCCGCTGGCCTACTCCGCCCGCTGCTTTACCGCGCGTTCGGAAGACCGCCCGAAAGATGAGTGTGAAACCTGCCGCATTAAGTATCCGAACgg                                                                                                                      <  2:646284/140‑1 (MQ=255)                       tCGTAACCAGTTTGAAGTGGAAGTCCTGAGCTACGGTCATCTGCCGCTGGCCTACTCCGCCCGCTGCTTTACCGCGCGTTCGGAAGACCGCCCGAAAGATGAGTGTGAAACCTGCCGCATTAAGTATCCGAACGGGCGCa                                                                                                                 <  1:675079/140‑1 (MQ=255)                       tCGTAACCAGTTTGAAGTGGAAGTCCTGAGCTACGGTCATCTGCCGCTGGCCTACTCCGCCCGCTGCTTTACCGCGCGTTCGGAAGACCGCCCGAAAGATGAGTGTGAAACCTGCCGCATTAAGTATCCGAACGGGCGCa                                                                                                                 >  2:174957/1‑140 (MQ=255)                         gTAACCAGTTTGAAGTGGAAGTCCTGAGCTACGGTCATCTGCCGCTGGCCTACTCCGCCCGCTGCTTTACCGCGCGTTCGGAAGACCGCCCGAAAGATGAGTGTGAAACCTGCCGCATTAAGTATCCGAACGGGCGCAAc                                                                                                               >  1:1271076/1‑140 (MQ=255)                         gTAACCAGTTTGAAGTGGAAGTCCTGAGCTACGGTCATCTGCCGCTGGCCTACTCCGCCCGCTGCTTTACCGCGCGTTCGGAAGACCGCCCGAAAGATGAGTGTGAAACCTGCCGCATTAAGTATCCGAACGGGCGCAAc                                                                                                               >  1:1199460/1‑140 (MQ=255)                            aCCAGTTTGAAGTGGAAGTCCTGAGCTACGGTCATCTGCCGCTGGCCTACTCCGCCCGCTGCTTTACCGCGCGTTCGGAAGACCGCCTGAAAGATGAGTGTGAAACCTGCCGCATTAAGTATCCGAACGGGCGCAACGTg                                                                                                            <  2:1109264/140‑1 (MQ=255)                            aCCAGTTTGAAGTGGAAGTCCTGAGCTACGGTCATCTGCCGCTGGCCTACTCCGCCCGCTGCTTTACCGCGCGTTCGGAAGACCGCCCGAAAGATGAGTGTGAAACCTGCCGCATTAAGTATCCGAACGGGCGCAACGTg                                                                                                            <  1:174957/140‑1 (MQ=255)                                gTTTGAAGTGGAAGTCCTGAGCTACGGTCATCTGCCGCTGGCCTACTCCGCCCGCTGCTTTACCGCGCGTTCGGAAGACCGCCCGAAAGATGAGTGTGAAACCTGCCGCATTAAGTATCCGAACGGGCGCAACGTGCTGt                                                                                                        <  2:1271076/140‑1 (MQ=255)                                 tttGAAGTGGAAGTCCTGAGCTACGGTCATCTGCCGCTGGCCTACTCCGCCCGCTGCTTTACCGCGCGTTCGGAAGACCGCCCGAAAGATGAGTGTGAAACCTGCCGCATTAAGTATCCGAACGGGCGCAACGTGCTGTc                                                                                                       >  1:337825/1‑140 (MQ=255)                                 tttGAAGTGGAAGTCCTGAGCTACGGTCATCTGCCGCTGGCCTACTCCGCCCGCTGCTTTACCGCGCGTTCGGAAGACCGCCCGAAAGATGAGTGTGAAACCTGCCGCATTAAGTATCCGAACGGGCGCAACGTGCTGTc                                                                                                       >  1:578734/1‑140 (MQ=255)                                       gTGGAAGTCCTGAGCTACGGTCATCTGCCGCTGGCCTACTCCGCCCGCTGCTTTACCGCGCGTTCGGAAGACCGCCCGAAAGATGAGTGTGAAACCTGCCGCATTAAGTATCCGAACGGGCGCAACGTGCTGTCGCAGGa                                                                                                 <  1:1404222/140‑1 (MQ=255)                                       gTGGAAGTCCTGAGCTACGGTCATCTGCCGCTGGCCTACTCCGCCCGCTGCTTTACCGCGCGTTCGGAAGACCGCCCGAAAGATGAGTGTGAAACCTGCCGCATTAAGTATCCGAACGGGCGCAACGTGCTGTCGCAGGa                                                                                                 <  2:337825/140‑1 (MQ=255)                                         ggAAGTCCTGAGCTACGGTCATCTGCCGCTGGCCTACTCCGCCCGCTGCTTTACCGCGCGTTCGGAAGACCGCCCGAAAGATGAGTGTGAAACCTGCCGCATTAAGTATCCGAACGGGCGCAACGTGCTGTCGCAGGaaa                                                                                               <  2:4454/140‑1 (MQ=255)                                                cTGAGCTACGGTCATCTGCCGCTGGCCTACTCCGCCCGCTGCTTTACCGCGCGTTCGGAAGACCGCCCGAAAGATGAGTGTGAAACCTGCCGCATTAAGTATCCGAACGGGCGCAACGTGCTGTCGCAGGAAAACcaaca                                                                                        <  2:12056/140‑1 (MQ=255)                                                cTGAGCTACGGTCATCTGCCGCTGGCCTACTCCGCCCGCTGCTTTACCGCGCGTTCGGAAGACCGCCCGAAAGATGAGTGTGAAACCTGCCGCATTAAGTATCCGAACGGGCGCAACGTGCTGTCGCAGGAAAACcaaca                                                                                        <  2:1199460/140‑1 (MQ=255)                                                cTGAGCTACGGTCATCTGCCGCTGGCCTACTCCGCCCGCTGCTTTACCGCGCGTTCGGAAGACCGCCCGAAAGATGAGTGTGAAACCTGCCGCATTAAGTATCCGAACGGGCGCAACGTGCTGTCGCAGGAAAACcaaca                                                                                        <  1:1207570/140‑1 (MQ=255)                                                  gAGCTACGGTCATCTGCCGCTGGCCTACTCCGCCCGCTGCTTTACCGCGCGTTCGGAAGACCGCCCGAAAGATGAGTGTGAAACCTGCCGCATTAAGTATCCGAACGGGCGCAACGTGCTGTCGCAGGAAAACCAACAAg                                                                                      <  1:205523/140‑1 (MQ=255)                                                     cTACGGTCATCTGCCGCTGGCCTACTCCGCCCGCTGCTTTACCGCGCGTTCGGAAGACCGCCCGAAAGATGAGTGTGAAACCTGCCGCATTAAGTATCCGAACGGGCGCAACGTGCTGTCGCAGGAAAACCAACAAgtgt                                                                                   <  2:366601/140‑1 (MQ=255)                                                          gTCATCTGCCGCTGGCCTACTCCGCCCGCTGCTTTACCGCGCGTTCGGAAGACCGCCCGAAAGATGAGTGTGAAACCTGCCGCATTAAGTATCCGAACGGGCGCAACGTGCTGTCGCAGGAAAACCAACAAGTGTTTGTa                                                                              <  2:869690/140‑1 (MQ=255)                                                               cTGCCGCTGGCCTACTCCGCCCGCTGCTTTACCGCGCGTTCGGAAGACCGCCCGAAAGATGAGTGTGAAACCTGCCGCATTAAGTATCCGAACGGGCGCAACGTGCTGTCGCAGGAAAACCAACAAGTGTTTGTACTCaa                                                                         >  1:892295/1‑140 (MQ=255)                                                                tGCCGCTGGCCTACTCCGCCCGCTGCTTTACCGCGCGTTCGGAAGACCGCCCGAAAGATGAGTGTGAAACCTGCCGCATTAAGTATCCGAACGGGCGCAACGTGCTGTCGCAGGAAAACCAACAAGTGTTTGTACTCAAt                                                                        <  1:1336161/140‑1 (MQ=255)                                                                 gCCGCTGGCCTACTCCGCCCGCTGCTTTACCGCGCGTTCGGAAGACCGCCCGAAAGATGAGTGTGAAACCTGCCGCATTAAGTATCCGAACGGGCGCAACGTGCTGTCGCAGGAAAACCAACAAGTGTTTGTACTCAATg                                                                       <  2:1343857/140‑1 (MQ=255)                                                                      tGGCCTACTCCGCCCGCTGCTTTACCGCGCGTTCGGAAGACCGCCCGAAAGATGAGTGTGAAACCTGCCGCATTAAGTATCCGAACGGGCGCAACGTGCTGTCGCAGGAAAACCAACAAGTGTTTGTACTCAATGGCAtt                                                                  <  2:892295/140‑1 (MQ=255)                                                                                  cccgcTGCTTTACCGCGCGTTCGGAAGACCGCCCGAAAGATGAGTGTGTAACCTGCCGCATTAAGTATCCGAACGGGCGCAACGTGCTGTCGCAGGAAAACCAACAAGTGTTTGTACTCAAt                                                                        <  2:1235208/122‑1 (MQ=255)                                                                                  cccgcTGCTTTACCGCGCGTTCGGAAGACCGCCCGAAAGATGAGTGTGAAACCTGCCGCATTAAGTATCCGAACGGGCGCAACGTGCTGTCGCAGGAAAACCAACAAGTGTTTGTACTCAAt                                                                        >  1:1235208/1‑122 (MQ=255)                                                                                  cccgcTGCTTTACCGCGCGTTCGGAAGACCGCCCGAAAGATGAGTGTGAAACCTGCCGCATTAAGTATCCGAACGGGCGCAACGTGCTGTCGCAGGAAAACCAACAAGTGTTTGTACTCAATGGCATTCAGACCATGAGc                                                      >  2:171815/1‑140 (MQ=255)                                                                                  cccgcTGCTTTACCGCGCGTTCGGAAGACCGCCCGAAAGATGAGTGTGAAACCTGCCGCATTAAGTATCCGAACGGGCGCAACGTGCTGTCGCAGGAAAACCAACAAGTGTTTGTACTCAATGGCATTCAGACCATGAGc                                                      >  2:32924/1‑140 (MQ=255)                                                                                  cccgcTGCTTTACCGCGCGTTCGGAAGACCGCCCGAAAGATGAGTGTGAAACCTGCCGCATTAAGTATCCGAACGGGCGCAACGTGCTGTCGCAGGAAAACCAACAAGTGTTTGTACTCAATGGCATTCAGACCATGAGc                                                      >  1:827583/1‑140 (MQ=255)                                                                                  cccgcTGCTTTACCGCGCGTTCGGAAGACCGCCCGAAAGATGAGTGTGAAACCTGCCGCATTAAGTATCCGAACGGGCGCAACGTGCTGTCGCAGGAAAACCAACAAGTGTTTGTACTCAATGGCATTCAGACCATGAGc                                                      >  2:491462/1‑140 (MQ=255)                                                                                  cccgcTGCTTTACCGCGCGTTCGGAAGACCGCCCGAAAGATGAGTGTGAAACCTGCCGCATTAAGTATCCGAACGGGCGCAACGTGCTGTCGCAGGAAAACCAACAAGTGTTTGTACTCAATGGCATTCAGACCATGAGc                                                      >  1:1406009/1‑140 (MQ=255)                                                                                  cccgcTGCTTTACCGCGCGTTCGGAAGACCGCCCGAAAGATGAGTGTGAAACCTGCCGCATTAAGTATCCGAACGGGCGCAACGTGCTGTCGCAGGAAAACCAACAAGTGTTTGTACTCAATGGCATTCAGACCATGAGc                                                      >  1:1011538/1‑140 (MQ=255)                                                                                  cccgcTGCTTTACCGCGCGTTCGGAAGACCGCCCGAAAGATGAGTGTGAAACCTGCCGCATTAAGGATCCGAACGGGCGCAACGTGCTGTCGCAGGAAAACCAACAAGTGTTTGTACTCAATGGCATTCAGACCATGAGc                                                      >  1:1240356/1‑140 (MQ=255)                                                                                   ccgcTGCTTTACCGCGCGTTCGGAAGACCGCCCGAAAGATGAGTGTGAAACCTGCCGCATTAAGTATCCGAACGGGCGCAACGTGCTGTCGCAGGAAAACCAACAAGTGTTTGTACTCAATGGCATTCAGACCATGAGCg                                                     <  1:171815/140‑1 (MQ=255)                                                                                     gctgctTTACCGCGCGTTCGGAAGACCGCCCGAAAGATGAGTGTGAAACCTGCCGCATTAAGTATCCGAACGGGCGCAACGTGCTGTCGCAGGAAAACCAACAAGTGTTTGTACTCAATGGCATTCAGACCATGAGCGGc                                                   <  2:1011538/140‑1 (MQ=255)                                                                                      ctgctTTACCGCGCGTTCGGAAGACCGCCCGAAAGATGAGTGTGAAACCTGCCGCATTAAGTATCCGAACGGGCGCAACGTGCTGTCGCAGGAAAACCAACAAGTGTTTGTACTCAATGGCATTCAGACCATGAGCGGCt                                                  <  1:32924/140‑1 (MQ=255)                                                                                       tgctTTACCGCGCGTTCGGAAGACCGCCGGAAAGATGAGTGTGAAACCTGCCGCATTAAGTATCCGAACGGGCGCAACGTGCTGTCGCAGGAAAACCAACAAGTGTTTGTACTCAATGGCATTCAGACCATGAGCGGCTa                                                 <  2:739303/140‑1 (MQ=255)                                                                                        gctTTACCGCGCGTTCGGAAGACCGCCCGAAAGATGAGTGTGAAACCTGCCGCATTAAGTATCCGAACGGGCGCAACGTGCTGTCGCAGGAAAACCAACAAGTGTTTGTACTCAATGGCATTCAGACCATGAGCGGCTAc                                                <  2:1240356/140‑1 (MQ=255)                                                                                         ctTTACCGCGCGTTCGGAAGACCGCCCGAAAGATGAGTGTGAAACCTGCCGCATTAAGTATCCGAACGGGCACAACGTGCTGTCGCAGGAAAACCAACAAGTGTTTGTACTCAATGGCATTCAGACCATGAGCGGCTACg                                               <  1:849368/140‑1 (MQ=255)                                                                                          tttACCGCGCGTTCGGAAGACCGCCCGAAAGATGAGTGTGAAACCTGCCGCATTAAGTATCCGAACGGGCGCAACGTGCTGTCGCAGGAAAACCAACAAGTGTTTGTACTCAATGGCATTCAGACCATGAGCGGCTACGt                                              >  2:799240/1‑140 (MQ=255)                                                                                              ccGCGCGTTCGGAAGACCGCCCGAAAGATGAGTGTGAAACCTGCCGCATTAAGTATCCGAACGGGCGCAACGTGCTGTCGCAGGAAAACCAACAAGTGTTTGTACTCAATGGCATTCAGACCATGAGCGGCTACGTTTAc                                          <  1:1082133/140‑1 (MQ=255)                                                                                                 cgcgTTCGGAAGACCGCCCGAAAGATGAGTGTGAAACCTGCCGCATTAAGTATCCGAACGGGCGCAACGTGCTGTCGCAGGAAAACCAACAAGTGTTTGTACTCAATGGCATTCAGACCATGAGCGGCt                                                  >  1:64194/1‑129 (MQ=255)                                                                                                 cgcgTTCGGAAGACCGCCCGAAAGATGAGTGTGAAACCTGCCGCATTAAGTATCCGAACGGGCGCAACGTGCTGTCGCAGGAAAACCAACAAGTGTTTGTACTCAATGGCATTCAGACCATGAGCGGCt                                                  <  2:64194/129‑1 (MQ=255)                                                                                                 cgcgTTCGGAAGACCGCCCGAAAGATGAGTGTGAAACCTGCCGCATTAAGTATCCGAACGGGCGCAACGTGCTGTCGCAGGAAAACCAACAAGTGTTTGTACTCAATGGCATTCAGACCATGAGCGGCTACGTTTACAAc                                       >  2:437759/1‑140 (MQ=255)                                                                                                          aaGACCGCCCGAAAGATGAGTGTGAAACCTGCCGCATTAAGTATCCGAACGGGCGCAACGTGCTGTCGCAGGAAAACCAACAAGTGTTTGTACTCAATGGCATTCAGACCATGAGCGGCTACGTTTACAACCTCGGTAAc                              <  2:1148623/140‑1 (MQ=255)                                                                                                           aGACCGCCCGAAAGATGAGTGTGAAACCTGCCGCATTAAGTATCCGAACGGGCGCAACGTGCTGTCGCAGGAAAACCAACAAGTGTTTGTACTCAATGGCATTCAGACCATGAGCGGCTACGTTTACAACCTCGGTAACg                             <  2:578734/140‑1 (MQ=255)                                                                                                               cGCCCGAAAGATGAGTGTGAAACCTGCCGCATTAAGTATCCGAACGGGCGCAACGTGCTGTCGCAGGAAAACCAACAAGTGTTTGTACTCAATGGCATTCAGACCATGAGCGGCTACGTTTACAACCTCGGTAACGAGCt                         >  1:867885/1‑140 (MQ=255)                                                                                                                gCCCGAAAGATGAGTGTGAAACCTGCCGCATTAAGTATCCGAACGGGCGCAACGTGCTGTCGCAGGAAAACCAACAAGTGTTTGTACTCAATGGCATTCAGACCATGAGCGGCTACGTTTACAACCTCGGTAACGAGCTg                        >  2:16880/1‑140 (MQ=255)                                                                                                                gCCCGAAAGATGAGTGTGAAACCTGCCGCATTAAGTATCCGAACGGGCGCAACGTGCTGTCGCAGGAAAACCAACAAGTGTTTGTACTCAATGGCATTCAGACCATGAGCGGCTACGTTTACAACCTCGGTAACGAGCTg                        >  1:69397/1‑140 (MQ=255)                                                                                                                 cccGAAAGATGAGTGTGAAACCTGCCGCATTAAGTATCCGAACGGGCGCAACGTGCTGTCGCAGGAAAACCAACAAGTGTTTGTACTCAATGGCATTCAGACCATGAGCGGCTACGTTTACAACCTCGGTAACGAGCTgg                       >  2:1413295/1‑140 (MQ=255)                                                                                                                 cccGAAAGATGAGTGTGAAACCTGCCGCATTAAGTATCCGAACGGGCGCAACGTGCTGTCGCAGGAAAACCAACAAGTGTTTGTACTCAATGGCATTCAGACCATGAGCGGCTACGTTTACAACCTCGGTAACGAGCTgg                       <  2:827583/140‑1 (MQ=255)                                                                                                                 cccGAAAGATGAGTGTGAAACCTGCCGCATTAAGTATCCGAACGGGCGCAACGTGCTGTCGCAGGAAAACCAACAAGTGTTTGTACTCAATGGCATTCAGACCATGAGCGGCTACGTTTACAACCTCGGTAACGAGCTgg                       >  2:1052445/1‑140 (MQ=255)                                                                                                                  ccGAAAGATGAGTGTGAAACCTGCCGCATTAAGTATCCGAACGGGCGCAACGTGCTGTCGCAGGAAAACCAACAAGTGTTTGTACTCAATGGCATTCAGACCATGAGCGGCTACGTTTACAACCTCGGTAAc                              <  1:268250/132‑1 (MQ=255)                                                                                                                  ccGAAAGATGAGTGTGAAACCTGCCGCATTAAGTATCCGAACGGGCGCAACGTGCTGTCGCAGGAAAACCAACAAGTGTTTGTACTCAATGGCATTCAGACCATGAGCGGCTACGTTTACAACCTCGGTAAc                              >  2:268250/1‑132 (MQ=255)                                                                                                                    gAAAGATGAGTGTGAAACCTGCCGCATTAAGTATCCGAACGGGCGCAACGTGCTGTCGCAGGAAAACCAACAAGTGTTTGTACTCAATGGCATTCAGACCATGAGCGGCTACGTTTACAACCTCGGTAACGAGCTGGCAt                    <  1:1404814/140‑1 (MQ=255)                                                                                                                    gAAAGATGAGTGTGAAACCTGCCGCATTAAGTATCCGAACGGGCGCAACGTGCTGTCGCAGGAAAACCAACAAGTGTTTGTACTCAATGGCATTCAGACCATGAGCGGCTACGTTTACAACCTCGGTAACGAGCTGGCAt                    <  1:1294657/140‑1 (MQ=255)                                                                                                                    gAAAGATGAGTGTGAAACCTGCCGCATTAAGTATCCGAACGGGCGCAACGTGCTGTCGCAGGAAAACCAACAAGTGTTTGTACTCAATGGCATTCAGACCATGAGCGGCTACGTTTACAACCTCGGTAACGAGCTGGCAt                    >  1:1126654/1‑140 (MQ=255)                                                                                                                     aaaGATGAGTGTGAAACCTGCCGCATTAAGTATCCGAACGGGCGCAACGTGCTGTCGCAGGAAAACCAACAAGTGTTTGTACTCAATGGCATTCAGACCATGAGCGGCTACGTTTACAACCTCGGTAACGAGCTGGCATc                   <  2:1129944/140‑1 (MQ=255)                                                                                                                              tgtgAAACCTGCCGCATTAAGTATCCGAACGGGCGCAACGTGCTGTCGCAGGAAAACCAACAAGTGTTTGTACTCAATGGCATTCAGACCATGAGCGGCTACGTTTACAACCTCGGTAACGAGCTGGCATCCATGCAggg          <  2:1126654/140‑1 (MQ=255)                                                                                                                              tgtgAAACCTGCCGCATTAAGTATCCGAACGGGCGCAACGTGCTGTCGCAGGAAAACCAACAAGTGTTTGTACTCAATGGCATTCAGACCATGAGCGGCTACGTTTACAACCTCGGTAACGAGCTGGCATCCATGCAggg          <  1:491462/140‑1 (MQ=255)                                                                                                                              tgtgAAACCTGCCGCATTAAGTATCCGAACGGGCGCAACGTGCTGTCGCAGGAAAACCAACAAGTGTTTGTACTCAATGGCATTCAGACCATGAGCGGCTACGTTTACAACCTCGGTAACGAGCTGGCATCCATGCAggg          >  1:196605/1‑140 (MQ=255)                                                                                                                              tgtgAAACCTGCCGCATTAAGTATCCGAACGGGCGCAACGTGCTGTCGCAGGAAAACCAACAAGTGTTTGTACTCAATGGCATTCAGACCATGAGCGGCTACGTTTACAACCTCGGTAACGAGCTGGCATCCATGCAggg          <  2:867885/140‑1 (MQ=255)                                                                                                                                  aaaCCTGCCGCATTAAGTATCCGAACGGGCGCAACGTGCTGTCGCAGGAAAACCAACAAGTGTTTGTACTCAATGGCATTCAGACCATGAGCGGCTACGTTTACAACCTCGGTAACGAGCTGGCATCCATGCAGGGCCTg      >  2:694467/1‑140 (MQ=255)                                                                                                                                      ctgccgCATTAAGTATCCGAACGGGCGCAACGTGCTGTCGCAGGAAAACCAACAAGTGTTTGTACTCAATGGCATTCAGACCATGAGCGGCTACGTTTACAACCTCGGTAACGAGCTGGCATCCATGCAGGGCCTGGTTg  >  2:459984/1‑140 (MQ=255)                                                                                                                                          |                                                                                                                                        ATCAGTGCGATGAGCTGGGCATTCGTAACCAGTTTGAAGTGGAAGTCCTGAGCTACGGTCATCTGCCGCTGGCCTACTCCGCCCGCTGCTTTACCGCGCGTTCGGAAGACCGCCCGAAAGATGAGTGTGAAACCTGCTGCATTAAGTATCCGAACGGGCGCAACGTGCTGTCGCAGGAAAACCAACAAGTGTTTGTACTCAATGGCATTCAGACCATGAGCGGCTACGTTTACAACCTCGGTAACGAGCTGGCATCCATGCAGGGCCTGGTTG  >  NC_000913/3302940‑3303212

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑