Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,359,761	T→C	G188G (GGT→GGC)	gltD →	glutamate synthase subunit GltD

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,359,761	0	T	C	100.0%	308.6 / NA	84	G188G (GGT→GGC)	gltD	glutamate synthase subunit GltD
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (35/49);  total (35/49)

AAACAGACCGGTAAAAAAGTGGCGATTATCGGCGCAGGCCCGGCAGGTCTGGCGTGTGCGGATGTCCTGACGCGTAACGGCGTAAAAGCCGTTGTCTTCGACCGTCATCCAGAAATTGGCGGGCTGCTGACCTTCGGTATTCCGGCCTTCAAGCTGGAAAAAGAGGTAATGACGCGTCGCCGTGAAATCTTCACCGGCATGGGTATTGAATTCAAACTCAATACCGAAGTGGGCCGCGACGTACAGCTGGACGATCTGCTGAGTGATTACGATG  >  NC_000913/3359624‑3359897                                                                                                                                          |                                                                                                                                         aaaCAGACCGGTAAAAAAGTGGCGATTATCGGCGCAGGCCCGGCAGGTCTGGCGTGTGCGGATGTCCTGACGCGTAACGGCGTAAAAGCCGTTGTCTTCGACCGTCATCCAGAAATTGGCGGGCTGTTGACCTTCGGCAt                                                                                                                                        >  1:860365/1‑140 (MQ=255) aaaCAGACCGGTAAAAAAGTGGCGATTATCGGCGCAGGCCCGGCAGGTCTGGCGTGTGCGGATGTCCTGACGCGTAACGGCGTAAAAGCCGTTGTCTTCGACCGTCATCCAGAAATTGGCGGGCTGCTGACCTTCGGCAt                                                                                                                                        >  1:74444/1‑140 (MQ=255) aaaCAGACCGGTAAAAAAGTGGCGATTATCGGCGCAGGCCCGGCAGGTCTGGCGTGTGCGGATGTCCTGACGCGTAACGGCGTAAAAGCCGTTGTCTTCGACCGTCATCCAGAAATTGGCGGGCTGCTGACCTTCGGCAt                                                                                                                                        >  2:442740/1‑140 (MQ=255) aaaCAGACCGGTAAAAAAGTGGCGATTATCGGCGCAGGCCCGGCAGGTCTGGCGTGTGCGGATGTCCTGACGCGTAACGGCGTAAAAGCCGTTGTCTTCGACCGTCATCCAGAAATTGGCGGGCTGCTGACCTTCGGCAt                                                                                                                                        >  1:157363/1‑140 (MQ=255)  aaCAGACCGGTAAAAAAGTGGCGATTATCGGCGCAGGCCCGGCAGGTCTGGCGTGTGCGGATGTCCTGACGCGTAACGGCGTAAAAGCCGTTGTCTTCGACCGTCATCCAGAAATTGGCGGGCTGCTGACCTTCGGCAtt                                                                                                                                       <  1:972365/140‑1 (MQ=255)     aGACCGGTAAAAAAGTGGCGATTATCGGCGCAGGCCCGGCAGGTCTGGCGTGTGCGGATGTCCTGACGCGTAACGGCGTAAAAGCCGTTGTCTTCGACCGTCATCCAGAAATTGGCGGGCTGCTGACCTTCGGCATTCCg                                                                                                                                    <  1:946458/140‑1 (MQ=255)       aCCGGTAAAAAAGTGGCGATTATCGGCGCAGGCCCGGCAGGTCTGGCGTGTGCGGATGTCCTGACGCGTAACGGCGTAAAAGCCGTTGTCTTCGACCGTCATCCAGAAATTGGCGGGCTGCTGACCTTCGGCATTCCGGc                                                                                                                                  >  2:596336/1‑140 (MQ=255)       aCCGGTAAAAAAGTGGCGATTATCGGCGCAGGCCCGGCAGGTCTGGCGTGTGCGGATGTCCTGACGCGTAACGGCGTAAAAGCCGTTGTCTTCGACCGTCATCCAGAAATTGGCGGGCTGCTGACCTTCGGCATTCCGGc                                                                                                                                  >  1:325432/1‑140 (MQ=255)       aCCGGTAAAAAAGTGGCGATTATCGGCGCAGGCCCGGCAGGTCTGGCGTGTGCGGATGTCCTGACGCGTAACGGCGTAAAAGCCGTTGTCTTCGACCGTCATCCAGAAATTGGCGGGCTGCTGACCTTCGGCATTCCGGc                                                                                                                                  >  2:359716/1‑140 (MQ=255)         cGGTAAAAAAGTGGCGATTATCGGCGCAGGCCCGGCAGGTCTGGCGTGTGCGGATGTCCTGACGCGTAACGGCGTAAAAGCCGTTGTCTTCGACCGTCATCCAGAAATTGGCGGGCTGCTGACCTTCGGCATTCCGGCCt                                                                                                                                <  2:441361/140‑1 (MQ=255)             aataaaGTGGCGATTATCGGCGCAGGCCCGGCAGGTCTGGCGTGTGCGGTTGTCCTGACGCGTAACGGCGTAAAAGCCGTTGTCTTCGACCGTCATCCAGAAATTGGCGGGCTGCTGCCCTTCGGCATTCCGGCCTTCaa                                                                                                                            <  2:958943/137‑1 (MQ=255)             aaaaaaGTGGCGATTATCGGCGCAGGCCCGGCAGGTCTGGCGTGTGCGGATGTCCTGACGCGTAACGGCGTAAAAGCCGTTGTCTTCGACCGTCATCCAGAAATTGGCGGGCTGCTGACCTTCGGCATTCCGGCCTTCaa                                                                                                                            <  1:361379/140‑1 (MQ=255)                aaaGTGGCGATTATCGGCGCAGGCCCGGCAGGTCTGGCGTGTGCGGATGTCCTGACGCGTAACGGCGTAAAAGCCGTTGTCTTCGACCGTCATCCAGAAATTGGCGGGCTGCTGACCTTCGGCATTCCGGCCTTCAAGCt                                                                                                                         <  1:596336/140‑1 (MQ=255)                 aaGTGGCGATTATCGGCGCAGGCCCGGCAGGTCTGGCGTGTGCGGATGTCCTGACGCGTAACGGCGTAAAAGCCGTTGTCTTCGACCGTCATCCAGAAATTGGCGGGCTGCTGACCTTCGGCATTCCGGCCTTCAAGCTg                                                                                                                        >  2:382804/1‑140 (MQ=255)                  aGTGGCGATTATCGGCGCAGGCCCGGCAGGTCTGGCGTGTGCGGATGTCCTGACGCGTAACGGCGTAAAAGCCGTTGTCTTCGACCGTCATCCAGAAATTGGCGGGCTGCTGACCTTCGGCATTCCGGCCTTCAAGCt                                                                                                                         >  2:196119/1‑138 (MQ=255)                  aGTGGCGATTATCGGCGCAGGCCCGGCAGGTCTGGCGTGTGCGGATGTCCTGACGCGTAACGGCGTAAAAGCCGTTGTCTTCGACCGTCATCCAGAAATTGGCGGGCTGCTGACCTTCGGCATTCCGGCCTTCAAGCt                                                                                                                         <  1:196119/138‑1 (MQ=255)                      gCGATTATCGGCGCAGGCCCGGCAGGTCTGGCGTGTGCGGATGTCCTGACGCGTAACGGCGTAAAAGCCGTTGTCTTCGACCGTCATCCAGAAATTGGCGGGCTGCTGACCTTCGGCATTCCGGCCTTCAAGCTGGaaaa                                                                                                                   <  1:137760/140‑1 (MQ=255)                      gCGATTATCGGCGCAGGCCCGGCAGGTCTGGCGTGTGCGGATGTCCTGACGCGTAACGGCGTAAAAGCCGTTGTCTTCGACCGTCATCCAGAAATTGGCGGGCTGCTGACCTTCGGCATTCCGGCCTTCAAGCTGGaaaa                                                                                                                   >  2:105230/1‑140 (MQ=255)                      gCGATTATCGGCGCAGGCCCGGCAGGTCTGGCGTGTGCGGATGTCCTGACGCGTAACGGCGTAAAAGCCGTTGTCTTCGACCGTCATCCAGAAATTGGCGGGCTGCTGACCTTCGGCATTCCGGCCTTCAAGCTGGaaaa                                                                                                                   <  2:74444/140‑1 (MQ=255)                      gCGATTATCGGCGCAGGCCCGGCAGGTCTGGCGTGTGCGGATGTCCTGACGCGTAACGGCGTAAAAGCCGTTGTCTTCGACCGTCATCCAGAAATTGGCGGGCTGCTGACCTTCGGCATTCCGGCCTTCAAGCTGGaaaa                                                                                                                   >  1:1139183/1‑140 (MQ=255)                         aTTATCGGCGCAGGCCCGGCAGGTCTGGCGTGTGCGGATGTCCTGACGCGTAACGGCGTAAAAGCCGTTGTCTTCGACCGTCATCCAGAAATTGGCGGGCTGCTGACCTTCGGCATTCCGGCCTTCAAGCTGGAAAAaga                                                                                                                <  1:382804/140‑1 (MQ=255)                                   cAGGCCCGGCAGGTCTGGCGTGTGCGGATGTCCTGACGCGTAACGGCGTAAAAGCCGTTGTCTTCGACCGTCATCCAGAAATTGGCGGGCTGCTGACCTTCGGCATTCCGGCCTTCAAGCTGGAAAAAGAGGTAATGAcg                                                                                                      >  1:1122383/1‑140 (MQ=255)                                   cAGGCCCGGCAGGTCTGGCGTGTGCGGATGTCCTGACGCGTAACGGCGTAAAAGCCGTTGTCTTCGACCGTCATCCAGAAATTGGCGGGCTGCTGACCTTCGGCATTCCGGCCTTCAAGCTGGAAAAAGAGGTAATGAcg                                                                                                      >  2:314491/1‑140 (MQ=255)                                     ggCCCGGCAGGTCTGGCGTGTGCGGATGTCCTGACGCGTAACGGCGTAAAAGCCGTTGTCTTCGACCGTCATCCAGAAATTGGCGGGCTGTTGACCTTCGGCATTCCGGCCTTCAAGCTGGAAAAAGAGGTAATGAcgcg                                                                                                    <  2:860365/140‑1 (MQ=255)                                           gCAGGTCTGGCGTGTGCGGATGTCCTGACGCGTAACGGCGTAAAAGCCGTTGTCTTCGACCGTCATCCAGAAATTGGCGGGCTGCTGACCTTCGGCATTCCGGCCTTCAAGCTGGAAAAAGAGGTAATGACGCGTCGCCg                                                                                              >  1:894617/1‑140 (MQ=255)                                           gCAGGTCTGGCGTGTGCGGATGTCCCGACGCGTAACGGCGTAAAAGCCGTTGTCTTCGACCGTCATCCAGAAATTGGCGGGCTGCTGACCTTCGGCATTCCGGCCTTCAAGCTGGAAAAAGAGGTAATGACGCGTCGCCg                                                                                              >  2:1026561/1‑140 (MQ=255)                                             aGGTCTGGCGTGTGCGGATGTCCTGACGCGTAACGGCGTAAAAGCCGTTGTCTTCGACCGTCATCCAGAAATTGGCGGGCTGCTGACCTTCGGCATTCCGGCCTTCAAGCTGGAAAAAGAGGTAATGACGCGTCGCCGTg                                                                                            <  2:1139183/140‑1 (MQ=255)                                              ggTCTGGCGTGTGCGGATGTCCTGACGCGTAACGGCGTAAAAGCCGTTGTCTTCGACCGTCATCCAGAAATTGGCGGGCTGCTGACCTTCGGCATTCCGGCCTTCAAGCTGGAAAAAGAGGTAATGACGCGTCGCCGTGa                                                                                           <  1:756516/140‑1 (MQ=255)                                                tCTGGCGTGTGCGGATGTCCTGACGCGTAACGGCGTAAAAGCCGTTGTCTTCGACCGTCATCCAGAAATTGGCGGGCTGCTGACCTTCGGCATTCCGGCCTTCAAGCTGGAAAAAGAGGTAATGACGCGTCGCCGTGaaa                                                                                         >  2:608217/1‑140 (MQ=255)                                                  tGGCGTGTGCGGATGTCCTGACGCGTAACGGCGTAAAAGCCGTTGTCTTCGACCGTCATCCAGAAATTGGCGGGCTGCTGACCTTCGGCATTCCGGCCTTCAAGCTGGAAAAAGAGGTAATGACGCGTCGCCGTg                                                                                            >  1:598072/1‑135 (MQ=255)                                                  tGGCGTGTGCGGATGTCCTGACGCGTAACGGCGTAAAAGCCGTTGTCTTCGACCGTCATCCAGAAATTGGCGGGCTGCTGACCTTCGGCATTCCGGCCTTCAAGCTGGAAAAAGAGGTAATGACGCGTCGCCGTg                                                                                            <  2:598072/135‑1 (MQ=255)                                                    gCGTGTGCGGATGTCCTGACGCGTAACGGCGTAAAAGCCGTTGTCTTCGACCGTCATCCAGAAATTGGCGGGCTGCTGACCTTCGGCATTCCGGCCTTCAAGCTGGAAAAAGAGGTAATGACGCGTCGCCGTGAAATCtt                                                                                     >  2:61174/1‑140 (MQ=255)                                                      gtgtGCGGATGTCCTGACGCGTAACGGCGTAAAAGCCGTTGTCTTCGACCGTCATCCAGAAATTGGCGGGCTGCTGACCTTCGGCATTCCGGCCTTCAAGCTGGAAAAAGAGGTAATGACGCGTCGCCGTGAAATCTTCa                                                                                   <  2:1417348/140‑1 (MQ=255)                                                      gtgtGCGGATGTCCTGACGCGTAACGGCGTAAAAGCCGTTGTCTTCGACCGTCATCCAGAAATTGGCGGGCTGCTGACCTTCGGCATTCCGGCCTTCAAGCTGGAAAAAGAGGTAATGACGCGTCGCCGTGAAATCTTCa                                                                                   <  2:633037/140‑1 (MQ=255)                                                      gtgtGCGGATGTCCTGACGCGTAACGGCGTAAAAGCCGTTGTCTTCGACCGTCATCCAGAAATTGGCGGGCTGCTGACCTTCGGCATTCCGGCCTTCAAGCTGGAAAAAGAGGTAATGACGCGTCGCCGTGAAATCTTCa                                                                                   <  1:1166577/140‑1 (MQ=255)                                                      gtgtGCGGATGTCCTGACGCGTAACGGCGTAAAAGCCGTTGTCTTCGACCGTCATCCAGAAATTGGCGGGCTGCTGACCTTCGGCATTCCGGCCTTCAAGCTGGAAAAAGAGGTAATGACGCGTCGCCGTGAAATCTTCa                                                                                   <  2:836049/140‑1 (MQ=255)                                                        ttgCGGATGTCCTGACGCGTAACGGCGTAAAAGCCGTTGTCTTCGACCGTCATCCAGAAATTGGCGGGCTGCTGACCTTCGGCATTCCGGCCTTCAAGCTGGAAAAAGAGGTAATGACGCGTCGCCGTGAAATCTTCAcc                                                                                 <  1:1026561/139‑1 (MQ=255)                                                        gtgCGGATGTCCTGACGCGTAACGGCGTAAAAGCCGTTGTCTTCGACCGTCATCCAGAAATTGGCGGGCTGCTGACCTTCGGCATTCCGGCCTTCAAGCTGGAAAAAGAGGTAATGACGCGTCGCCGTGAAATCTTCAcc                                                                                 <  2:325432/140‑1 (MQ=255)                                                        gtgCGGATGTCCTGACGCGTAACGGCGTAAAAGCCGTTGTCTTCGACCGTCATCCAGAAATTGGCGGGCTGCTGACCTTCGGCATTCCGGCCTTCAAGCTGGAAAAAGAGGTAATGACGCGTCGCCGTGAAATCTTCAcc                                                                                 <  1:314491/140‑1 (MQ=255)                                                        gtgCGGATGTCCTGACGCGTAACGGCGTAAAAGCCGTTGTCTTCGACCGTCATCCAGAAATTGGCGGGCTGCTGACCTTCGGCATTCCGGCCTTCAAGCTGGAAAAAGAGGTAATGACGCGTCGCCGTGAAATCTTCAcc                                                                                 <  1:1374985/140‑1 (MQ=255)                                                         tgCGGATGTCCTGACGCGTAACGGCGTAAAAGCCGTTGTCTTCGACCGTCATCCAGAAATTGGCGGGCTGCTGACCTTCGGCATTCCGGCCTTCAAGCTGGAAAAAGAGGTAATGACGCGTCGCCGTGAAATCTTCACCg                                                                                >  1:718678/1‑140 (MQ=255)                                                         tgCGGATGTCCTGACGCGTAACGGCGTAAAAGCCGTTGTCTTCGACCGTCATCCAGAAATTGGCGGGCTGCTGACCTTCGGCATTCCGGCCTTCAAGCTGGAAAAAGAGGTAATGACGCGTCGCCGTGAAATCTTCACCg                                                                                >  2:197597/1‑140 (MQ=255)                                                         tgCGGATGTCCTGACGCGTAACGGCGTAAAAGCCGTTGTCTTCGACCGTCATCCAGAAATTGGCGGGCTGCTGACCTTCGGCATTCCGGCCTTCAAGCTGGAAAAAGAGGTAATGACGCGTCGCCGTGAAATCTTCACCg                                                                                >  2:910945/1‑140 (MQ=255)                                                         tgCGGATGTCCTGACGCGTAACGGCGTAAAAGCCGTTGTCTTCGACCGTCATCCAGAAATTGGCGGGCTGCTGACCTTCGGCATTCCGGCCTTCAAGCTGGAAAAAGAGGTAATGACGCGTCGCCGTGAAATCTTCACCg                                                                                >  2:820729/1‑140 (MQ=255)                                                          gCGGATGTCCTGACGCGTAACGGCGTAAAAGCCGTTGTCTTCGACCGTCATCCAGAAATTGGCGGGCTGCTGACCTTCGGCATTCCGGCCTTCAAGCTGGAAAAAGAGGTAATGACGCGTCGCCGTGAAATCTTCACCgg                                                                               <  2:894617/140‑1 (MQ=255)                                                          gCGGATGTCCTGACGCGTAACGGCGTAAAAGCCGTTGTCTTCGACCGTCATCCAGAAATTGGCGGGCTGCTGACCTTCGGCATTCCGGCCTTCAAGCTGGAAAAAGAGGTAATGACGCGTCGCCGTGAAATCTTCACCgg                                                                               <  2:1104887/140‑1 (MQ=255)                                                           cGGATGTCCTGACGCGTAACGGCGTAAAAGCCGTTGTCTTCGACCGTCATCCAGAAATTGGCGGGCTGCTGACCTTCGGCATTCCGGCCTTCAAGCTGGAAAAAGAGGTAATGACGCGTCGCCGTGAAATCTTCACCGGc                                                                              <  1:197597/140‑1 (MQ=255)                                                           cGGATGTCCTGACGCGTAACGGCGTAAAAGCCGTTGTCTTCGACCGTCATCCAGAAATTGGCGGGCTGCTGACCTTCGGCATTCCGGCCTTCAAGCTGGAAAAAGAGGTAATGACGCGTCGCCGTGAAATCTTCACCGGc                                                                              <  1:820729/140‑1 (MQ=255)                                                            ggATGTCCTGACGCGTAACGGCGTAAAAGCCGTTGTCTTCGACCGTCATCCAGAAATTGGCGGGCTGCTGACCTTCGGCATTCCGGCCTTCAAGCTGGAAAAAGAGGTAATGACGCGTCGCCGTGAAATCTTCACCGGCa                                                                             <  2:1179404/140‑1 (MQ=255)                                                              aTGTCCTGACGCGTAACGGCGTAAAAGCCGTTGTCTTCGACCGTCATCCAGAAATTGGCGGGCTGCTGACCTTCGGCATTCCGGCCTTCAAGCTGGAAAAAGAGGTAATGACGCGTCGCCGTGAAATCTTCAcc                                                                                 <  2:106603/134‑1 (MQ=255)                                                              aTGTCCTGACGCGTAACGGCGTAAAAGCCGTTGTCTTCGACCGTCATCCAGAAATTGGCGGGCTGCTGACCTTCGGCATTCCGGCCTTCAAGCTGGAAAAAGAGGTAATGACGCGTCGCCGTGAAATCTTCAcc                                                                                 >  1:106603/1‑134 (MQ=255)                                                              aTGTCCTGACGCGTAACGGCGTAAAAGCCGTTGTCTTCGACCGTCATCCAGAAATTGGCGGGCTGCTGACCTTCGGCATTCCGGCCTTCAAGCTGGAAAAAGAGGTAATGACGCGTCGCCGTGAAATCTTCACCGGCATg                                                                           >  2:65091/1‑140 (MQ=255)                                                                  ccTGACGCGTAACGGCGTAAAAGCCGTTGTCTTCGACCGTCATCCAGAAATTGGCGGGCTGCTGACCTTCGGCATTCCGGCCTTCAAGCTGGAAAAAGAGGTAATGACGCGTCGCCGTGAAATCTTCACCGGCATGGGTa                                                                       <  1:65091/140‑1 (MQ=255)                                                                           tAACGGCGTAAAAGCCGTTGTCTTCGACCGTCATCCAGAAATTGGCGGGCTGCTGACCTTCGGCATTCCGGCCTTCAAGCTGGAAAAAGAGGTAATGACGCGTCGCCGTGAAATCTTCACCGGCATGGGTATTGAATTCa                                                              >  2:847279/1‑140 (MQ=255)                                                                           tAACGGCGTAAAAGCCGTTGTCTTCGACCGTCATCCAGAAATTGGCGGGCTGCTGACCTTCGGCATTCCGGCCTTCAAGCTGGAAAAAGAGGTAATGACGCGTCGCCGTGAAATCTTCACCGGCATGGGTATTGAATTCa                                                              >  1:912084/1‑140 (MQ=255)                                                                            aaCGGCGTAAAAGCCGTTGTCTTCGACCGTCATCCAGAAATTGGCGGGCTGCTGACCTTCGGCATTCCGGCCTTCAAGCTGGAAAAAGAGGTAATGACGCGTCGCCGTGAAATCTTCACCGGCATGGGTATTGAATTCaa                                                             >  2:996435/1‑140 (MQ=255)                                                                             aCGGCGTAAAAGCCGTTGTCTTCGACCGTCATCCAGAAATTGGCGGGCTGCTGACCTTCGGCATTCCGGCCTTCAAGCTGGAAAAAGAGGTAATGACGCGTCGCCGTGAAATCTTCACCGGCATGGGTATTGAATTCaaa                                                            <  1:1208418/140‑1 (MQ=255)                                                                               ggCGTAAAAGCCGTTGTCTTCGACCGTCATCCAGAAATTGGCGGGCTGCTGACCTTCGGCATTCCGGCCTTCAAGCTGGAAAAAGAGGTAATGACGCGTCGCCGTGAAATCTTCACCGGCATGGGTATTGAATTCAAACt                                                          <  1:989561/140‑1 (MQ=255)                                                                                 cGTAAAAGCCGTTGTCTTCGACCGTCATCCAGAAATTGGCGGGCTGCTGACCTTCGGCATTCCGGCCTTCAAGCTGG‑AAAAGAGGTAATGACGCGTTGCCGTGAAATCTTCACCGGCATGGGTATTGAATTCAAACTCaa                                                       >  1:389311/1‑140 (MQ=255)                                                                                  gTAAAAGCCGTTGTCTTCGACCGTCATCCAGAAATTGGCGGGCTGCTGACCTTCGGCATTCCGGCCTTCAAGCTGGAAAAAGAGGTAATGACGCGTCGCCGTGAAATCTTCACCGGCATGGGTATTGAATTCAAACTCaa                                                       >  1:1183010/1‑140 (MQ=255)                                                                                   tAAAAGCCGTTGTCTTCGACCGTCATCCAGAAATTGGCGGGCTGCTGACCTTCGGCATTCCGGCCTTCAAGCTGGAAAAAGAGGTAATGACGCGTCGCCGTGAAATCTTCACCGGCATGGGTATTGAATTCAAACTCAAt                                                      >  1:1012433/1‑140 (MQ=255)                                                                                            ttGTCTTCGACCGTCATCCAGAAATTGGCGGGCTGCTGACCTTCGGCATTCCGGCCTTCAAGCTGGAAAAAGAGGTAATGACGCGTCGCCGTGAAATCTTCACCGGCATGGGTATTGAATTCAAACTCAATACCGAAGTg                                             >  1:973840/1‑140 (MQ=255)                                                                                             tGTCTTCGACCGTCATCCAGAAATTGGCGGGCTGCTGACCTTCGGCATTCCGGCCTTCAAGCTGGAAAAAGAGGTAATGACGCGTCGCCGTGAAATCTTCACCGGCATGGGTATTGAATTCAAACTCAATACCGAAGTgg                                            >  2:143284/1‑140 (MQ=255)                                                                                                 ttCGACCGTCATCCAGAAATTGGCGGGCTGCTGACCTTCGGCATTCCGGCCTTCAAGCTGGAAAAAGAGGTAATGACGCGTCGCCGTGAAATCTTCACCGGCATGGGTATTGAATTCAAACTCAATACCGAAGTGGGCcg                                        >  2:307995/1‑140 (MQ=255)                                                                                                   cGACCGTCATCCAGAAATTGGCGGGCTGCTGACCTTCGGCATTCCGGCCTTCAAGCTGGAAAAAGAGGTAATGACGCGTCGCCGTGAAATCTTCACCGGCATGGGTATTGAATTCAAACTCAATACCGAAGTGGGCcgcg                                      <  1:442740/140‑1 (MQ=255)                                                                                                   cGACCGTCATCCAGAAATTGGCGGGCTGCTGACCTTCGGCATTCCGGCCTTCAAGCTGGAAAAAGAGGTAATGACGCGTCGCCGTGAAATCTTCACCGGCATGGGTATTGAATTCAAACTCAATACCGAAGTGGGCcgcg                                      <  1:847279/140‑1 (MQ=255)                                                                                                      ccGTCATCCAGAAATTGGCGGGCTGCTGACCTTCGGCATTCCGGCCTTCAAGCTGGAAAAAGAGGTAATGACGCGTCGCCGTGAAATCTTCACCGGCATGGGTATTGAATTCAAACTCAATACCGAAGTGGGCCGCGACg                                   <  2:157363/140‑1 (MQ=255)                                                                                                      ccGTCATCCAGAAATTGGCGGGCTGCTGACCTTCGGCATTCCGGCCTTCAAGCTGGAAAAAGAGGTAATGACGCGTCGCCGTGAAATCTTCACCGGCATGGGTATTGAATTCAAACTCAATACCGAAGTGGGCCGCGACg                                   <  2:757617/140‑1 (MQ=255)                                                                                                        gTCATCCAGAAATTGGCGGGCTGCTGACCTTCGGCATTCCGGCCTTCAAGCTGGAAAAAGAGGTAATGACGCGTCGCCGTGAAATCTTCACCGGCATGGGTATTGAATTCAAACTCAATACCGAAGTGGGCCGCGACGTa                                 <  1:307995/140‑1 (MQ=255)                                                                                                             ccAGAAATTGGCGGGCTGCTGACCTTCGGCATTCCGGCCTTCAAGCTGGAAAAAGAGGTAATGACGCGTCGCCGTGAAATCTTCACCGGCATGGGTATTGAATTCAAACTCAATACCGAAGTGGGCCGCGACGTACAGCt                            <  1:910945/140‑1 (MQ=255)                                                                                                             ccAGAAATTGGCGGGCTGCTGACCTTCGGCATTCCGGCCTTCAAGCTGGAAAAAGAGGTAATGACGCGTCGCCGTGAAATCTTCACCGGCATGGGTATTGAATTCAAACTCAATACCGAAGTGGGCCGCGACGTACAGCt                            <  1:996435/140‑1 (MQ=255)                                                                                                               aGAAATTGGCGGGCTGCTGACCTTCGGCATTCCGGCCTTCAAGCTGGAAAAAGAGGTAATGACGCGTCGCCGTGAAATCTTCACCGGCATGGGTATTGAATTCAAACTCAATACCGAAGTGGGCCGCGACGTACAGCTgg                          <  2:1012433/140‑1 (MQ=255)                                                                                                               aGAAATTGGCGGGCTGCTGACCTTCGGCATTCCGGCCTTCAAGCTGGAAAAAGAGGTAATGACGCGTCGCCGTGAAATCTTCACCGGCATGGGTATTGAATTCAAACTCAATACCGAAGTGGGCCGCGACGTACAGCTgg                          <  1:359716/140‑1 (MQ=255)                                                                                                                 aaaTTGGCGGGCTGCTGACCTTCGGCATTCCGGCCTTCAAGCTGGAAAAAGAGGTAATGACGCGTCGCCGTGAAATCTTCACCGGCATGGGTATTGAATTCAAACTCAATACCGAAGTGGGCCGCGACGTACAGCTGGAc                        <  2:718678/140‑1 (MQ=255)                                                                                                                   aTTGGCGGGCTGCTGACCTTCGGCATTCCGGCCTTCAAGCTGGAAAAAGAGGTAATGACGCGTCGCCGTGAAATCTTCATCGGCATGGGTATTGAATTCAAACTCAATACCGAAGTGGGCCGCGACGTACAGCTGGACGa                      >  1:928355/1‑140 (MQ=255)                                                                                                                    ttGGCGGGCTGCTGACCTTCGGCATTCCGGCCTTCAAGCTGGAAAAAGAGGTAATGACGCGTCGCCGTGAAATCTTCACCGGCATGGGTATTGAATTCAAACTCAATACCGAAGTGGGCCGCGACGTACAGCTGGACGAt                     <  2:1183010/140‑1 (MQ=255)                                                                                                                    ttGGCGGGCTGCTGACCTTCGGCATTCCGGCCTTCAAGCTGGAAAAAGAGGTAATGACGCGTCGCCGTGAAATCTTCACCGGCATGGGTATTGAATTCAAACTCAATACCGAAGTGGGCCGCGACGTACAGCTGGACGAt                     >  1:1221645/1‑140 (MQ=255)                                                                                                                     tGGCGGGCTGCTGACCTTCGGCATTCCAGCCTTCAAGCTGGAAAAAGAGGTAATGACGCGTCGCCGTGAAATCTTCACCGGCATGGGTATTGAATTCAAACTCAATACCGAAGTGGGCCGCGACGTACAGCTGGACGATc                    >  2:1072580/1‑140 (MQ=255)                                                                                                                      ggCGGGCTGCTGACCTTCGGCATTCCGGCCTTCAAGCTGGAAAAAGAGGTAATGACGCGTCGCCGTGAAATCTTCACCGGCATGGGTATTGAATTCAAACTCAATACCGAAGTGGGCCGCGACGTACAGCTGGACGATct                   >  2:812791/1‑140 (MQ=255)                                                                                                                         gggCTGCTGACCTTCGGCATTCCGGCCTTCAAGCTGGAAAAAGAGGTAATGACGCGTCGCCGTGAAATCTTCGCCGGCATGGGTATTGAATTCAAACTCAATACCGAAGTGGGCCGCGACGTACAGCTGGACGATctgct                <  2:1122383/140‑1 (MQ=255)                                                                                                                         gggCTGCTGACCTTCGGCATTCCGGCCTTCAAGCTGGAAAAAGAGGTAATGACGCGTCGCCGTGAAATCTTCACCGGCATGGGTATTGAATTCAAACTCAATACCGAAGTGGGCCGCGACGTACAGCTGGACGATctgct                <  1:1328446/140‑1 (MQ=255)                                                                                                                          ggCTGCTGACCTTCGGCATTCCGGCCTTCAAGCTGGAAAAAGAGGTAATGACGCGTCGCCGTGAAATCTTCACCGGCATGGGTATTGAATTCAAACTCAATACCGAAGTGGGCCGCGACGTACAGCTGGACGATctgctg               <  2:973840/140‑1 (MQ=255)                                                                                                                           gctgctGACCTTCGGCATTCCGGCCTTCAAGCTGGAAAAAGAGGTAATGACGCGTCGCCGTGAAATCTTCACCGGCATGGGTATTGAATTCAAACTCAATACCGAAGTGGGCCGCGACGTACAGCTGGACGATCTGCTGa              >  1:333560/1‑140 (MQ=255)                                                                                                                           gctgctGACCTTCGGCATTCCGGCCTTCAAGCTGGAAAAAGAGGTAATGACGCGTCGCCGTGAAATCTTCACCGGCATGGGTATTGAATTCAAACTCAATACCGAAGTGGGCCGCGACGTACAGCTGGACGATCTGCTGa              <  1:573552/140‑1 (MQ=255)                                                                                                                           gctgctGACCTTCGGCATTCCGGCCTTCAAGCTGGAAAAAGAGGTAATGACGCGTCGCCGTGAAATCTTCACCGGCATGGGTATTGAATTCAAACTCAATACCGAAGTGGGCCGCGACGTACAGCTGGACGATCTGCTGa              <  1:61174/140‑1 (MQ=255)                                                                                                                                    cTTCGGCATTCCGGCCTTCAAGCTGGAAAAAGAGGTAATGACGCGTCGCCGTGAAATCTTCACCGGCATGGGTATTGAATTCAAACTCAATACCGAAGTGGGCCGCGACGTACAGCTGGACGATCTGCTGAGTGATTACg     <  2:912084/140‑1 (MQ=255)                                                                                                                                       cGGCATTCCGGCCTTCAAGCTGGAAAAAGAGGTAATGACGCGTCGCCGTGAAATCTTCACCGGCATGGGTATTGAATTCAAACTCAATACCGAAGTGGGCCGCGACGTACAGCTGGACGATCTGCTGAGTGATTACGATg  <  1:608217/140‑1 (MQ=255)                                                                                                                                          |                                                                                                                                         AAACAGACCGGTAAAAAAGTGGCGATTATCGGCGCAGGCCCGGCAGGTCTGGCGTGTGCGGATGTCCTGACGCGTAACGGCGTAAAAGCCGTTGTCTTCGACCGTCATCCAGAAATTGGCGGGCTGCTGACCTTCGGTATTCCGGCCTTCAAGCTGGAAAAAGAGGTAATGACGCGTCGCCGTGAAATCTTCACCGGCATGGGTATTGAATTCAAACTCAATACCGAAGTGGGCCGCGACGTACAGCTGGACGATCTGCTGAGTGATTACGATG  >  NC_000913/3359624‑3359897

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑