Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,685,542	(C)6→5	coding (1047/1404 nt)	fumC ←	fumarase C

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,685,537	0	C	.	100.0%	85.4 / NA	21	coding (1052/1404 nt)	fumC	fumarase C
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base . (9/12);  total (9/12)

TCGGTTCAATACCCACTGCGCAGTGTTTGTTAAAACTTTCCATGCCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGCGCCCCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTCACACTGTGTTGGGTTCACTTTCCCCGGCATGATTGAGCTGCCCGGCTCATTTTCC  >  NC_000913/1685409‑1685653                                                                                                                                 |                                                                                                                     tCGGTTCAATACCCACTGCGCAGTGTTTGTTAAAACTTTCCATGCCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGCG‑CCCCCATGTTGa                                                                                                          <  1:264329/140‑1 (MQ=255)                 tGCGCAGTGTTTGTTAAAACTTTCCATGCCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGCG‑CCCCCATGTTGATCGCCACGTCGTTccc                                                                                          <  2:227233/140‑1 (MQ=255)                  gcgcAGTGTTTGTTAAAACTTTCCATGCCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGCG‑CCCCCATGTTGATCGCCACGTCGTTccc                                                                                          >  1:329693/1‑139 (MQ=255)                  gcgcAGTGTTTGTTAAAACTTTCCATGCCATCTGCCAGCAACCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGCG‑CCCCCATGTTGATCGCCACGTCGTTccc                                                                                          <  2:329693/139‑1 (MQ=255)                        tgtttgttAAAACTTTCCATGCCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGCG‑CCCCCATGTTGATCGCCACGTCGTTCCCCATCAcc                                                                                   <  1:34001/140‑1 (MQ=255)                         gtttgttAAAACTTTCCATGCCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGCG‑CCCGCATGTTGATCGCCACGTCGTTCCCCATCACCt                                                                                  <  1:307920/140‑1 (MQ=255)                             gttAAAACTTTCCATGCCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGCG‑CCCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGAca                                                                              >  2:341402/1‑140 (MQ=255)                                  aaCTTTCCATGCCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGCG‑CCCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCaga                                                                         >  2:71807/1‑140 (MQ=255)                                  aaCTTTCCATGCCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGCG‑CCCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCaga                                                                         >  2:286875/1‑140 (MQ=255)                                     tttCCATGCCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGCG‑CCCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCa                                                                      <  1:222772/140‑1 (MQ=255)                                           tGCCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGCG‑CCCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTa                                                                <  1:71807/140‑1 (MQ=255)                                                     cAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGCG‑CCCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTcaca                                                      <  1:341402/140‑1 (MQ=255)                                                            cgcACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGCG‑CCCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTCACACTGTGtt                                               >  2:232829/1‑140 (MQ=255)                                                              cACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGCG‑CCCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTCACACTGTGTTgg                                             >  2:122765/1‑140 (MQ=255)                                                                          gAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGCG‑CCCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTCACACTGTGTTGGGTTCACTTTccc                                 <  1:232829/140‑1 (MQ=255)                                                                                           ttGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGCG‑CCCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTCACACTGTGTTGGGTTCACTTTCCCCGGCATGATTGAGCTGc                <  2:121278/140‑1 (MQ=255)                                                                                             ggACGGAAGACGTTCAGTTCAAAGTTACCGGAAGCG‑CCCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTCACACTGTGTTGGGTTCACTTTCCCCGGCATGATTGAGCTGccc              >  1:36503/1‑140 (MQ=255)                                                                                                   aaGACGTTCAGTTCAAAGTTACCGGAAGCG‑CCCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTCACACTGTGTTGGGTTCACTTTCCCCGGCATGATTGAGCTGCCCgg            >  2:324434/1‑136 (MQ=255)                                                                                                   aaGACGTTCAGTTCAAAGTTACCGGAAGCG‑CCCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTCACACTGTGTTGGGTTCACTTTCCCCGGCATGATTGAGCTGCCCgg            <  1:324434/136‑1 (MQ=255)                                                                                                   aaGACGTTCAGTTCAAAGTTACCGGAAGCG‑CCCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTCACACTGTGTTGGGTTCACTTTCCCCGGCATGATTGAGCTGCCCGGCTCa        >  1:55477/1‑140 (MQ=255)                                                                                                         ttcagttcaAAGTTACCGGAAGCG‑CCCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTCACACTGTGTTGGGTTCACTTTCCCCGGCATGATTGAGCTGCCCGGCTCATTTTcc  <  2:55477/140‑1 (MQ=255)                                                                                                                                 |                                                                                                                     TCGGTTCAATACCCACTGCGCAGTGTTTGTTAAAACTTTCCATGCCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGCGCCCCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTCACACTGTGTTGGGTTCACTTTCCCCGGCATGATTGAGCTGCCCGGCTCATTTTCC  >  NC_000913/1685409‑1685653

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑