Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,312,788	(T)6→5	intergenic (‑39/‑296)	ompC ← / → micF	outer membrane porin C/small regulatory RNA MicF

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,312,783	0	T	.	100.0%	86.1 / NA	21	intergenic (‑34/‑301)	ompC/micF	outer membrane porin C/small regulatory RNA MicF
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base . (8/13);  total (8/13)

GATCTAATTTGTTGCCGTCTTTGTTGTAAACTTCAGCAGCGTTTGCTGCGCCTGCTACCAGCAGAGCTGGGACCAGGAGGGACAGTACTTTAACTTTCATGTTATTAACCCTCTGTTATATGCCTTTATTTGCTTTTTTATGCCACTGCATACTGATTAACCCTCATTAATCAGTCGGCAAGTCCATTCTCCCCAAAAATGCAGAATAATCCAACACGAATATGATACTAAAACTTTTAAGATGTTTCATTTAT  >  NC_000913/2312650‑2312903                                                                                                                                      |                                                                                                                         gATCTAATTTGTTGCCGTCTTTGTTGTAAACTTCAGCAGCGTTTGCTGCGCCTGCTACCAGCAGAGCTGGGACCAGGAGGGACAGTACTTTAACTTTCATGTTATTAACCCTCTGTTATATGCCTTTATTTGCtttttat                                                                                                                    >  2:81335/1‑138 (MQ=255)  aTCTAATTTGTTGCCGTCTTTGTTGTAAACTTCAGCAGCGTTTGCTGCGCCTGCTACCAGCAGAGCTGGGACCAGGAGGGACAGTACTTTAACTTTCATGTTATTAACCCTCTGTTATATGCCTTTATTTGCtttttatg                                                                                                                   >  1:165841/1‑137 (MQ=255)  aTCTAATTTGTTGCCGTCTTTGTTGTAAACTTCAGCAGCGTTTGCTGCGCCTGCTACCAGCAGAGCTGGGACCAGGAGGGACAGTACTTTAACTTTCATGTTATTAACCCTCTGTTATATGCCTTTATTTGCtttttatg                                                                                                                   >  1:195665/1‑137 (MQ=255)  aTCTAATTTGTTGCCGTCTTTGTTGTAAACTTCAGCAGCGTTTGCTGCGCCTGCTACCAGCAGAGCTGGGACCAGGAGGGACAGTACTTTAACTTTCATGTTATTAACCCTCTGTTATATGCCTTTATTTGCtttttatg                                                                                                                   <  1:203663/140‑4 (MQ=255)  aTCTAATTTGTTGCCGTCTTTGTTGTAAACTTCAGCAGCGTTTGCTGCGCCTGCTACCAGCAGAGCTGGGACCAGGAGGGACAGTACTTTAACTTTCATGTTATTAACCCTCTGTTATATGCCTTTATTTGCtttttatg                                                                                                                   <  2:244398/140‑4 (MQ=255)  aTCTAATTTGTTGCCGTCTTTGTTGTAAACTTCAGCAGCGTTTGCTGCGCCTGCTACCAGCAGAGCTGGGACCAGGAGGGACAGTACTTTAACTTTCATGTTATTAACCCTCTGTTATATGCCTTTATTTGCtttttatg                                                                                                                   >  1:207846/1‑137 (MQ=255)  aTCTAATTTGTTGCCGTCTTTGTTGTAAACTTCAGCAGCGTTTGCTGCGCCTGCTACCAGCAGAGCTGGGACCAGGAGGGACAGTACTTTAACTTTCATGTTATTAACCCTCTGTTATATGCCTTTATTTGCtttttatg                                                                                                                   <  2:258290/140‑4 (MQ=255)     tAATTTGTTGCCGTCTTTGTTGTAAACTTCAGCAGCGTTTGCTGCGCCTGCTACCAGCAGAGCTGGGACCAGGAGGGACAGTACTTTAACTTTCATGTTATTAACCCTCTGTTATATGCCTTTATTTGC‑TTTTTATGCCa                                                                                                               <  1:128911/140‑1 (MQ=255)            ttgCCGTCTTTGTTGTAAACTTCAGCAGCGTTTGCTGCGCCTGCTACCAGCAGAGCTGGGACCAGGAGGGACAGTACTTTAACTTTCATGTTATTAACCCTCTGTTATATGCCTTTATTTGC‑TTTTTATGCCACTGCATa                                                                                                        <  2:165841/140‑1 (MQ=255)                 gTCTTTGTTGTAAACTTCAGCAGCGTTTGCTGCGCCTGCTACCAGCAGAGCTGGGACCAGGAGGGACAGTACTTTAACTTTCATGTTATTAACCCTCTGTTATATGCCTTTATTTGC‑TTTTTATGCCACTGCATACTGAt                                                                                                   <  2:219395/140‑1 (MQ=255)                 gTCTTTGTTGTAAACTTCAGCAGCGTTTGCTGCGCCTGCTACCAGCAGAGCTGGGACCAGGAGGGACAGTACTTTAACTTTCATGTTATTAACCCTCTGTTATATGCCTTTATTTGC‑TTTTTATGCCACTGCATACTGAt                                                                                                   <  1:252158/140‑1 (MQ=255)                    tttGTTGTAAACTTCAGCAGCGTTTGCTGCGCCTGCTACCAGCAGAGCTGGGACCAGGAGGGACAGTACTTTAACTTTCATGTTATTAACCCTCTGTTATATGCCTTTATTTGC‑TTTTTATGCCACTGCATACTGATTaa                                                                                                <  2:195665/140‑1 (MQ=255)                                  cagcagCGTTTGCTGCGCCTGCTACCAGCAGAGCGGGGACCAGGAGGGACAGTACTTTAACTTTCATGTTATTAACCCTCTGTTATATGCCTTTATTTGC‑TTTTTATGCCACTGCATACTGATTAACCCTCATTAATCAg                                                                                  >  2:314711/1‑140 (MQ=255)                                     cagcGTTTGCTGCGCCTGCTACCAGCAGAGCTGGGACCAGGAGGGACAGTACTTTAACTTTCATGTTATTAACCCTCTGTTATATGCCTTTATTTGC‑TTTTTATGCCACTGCATACTGATTAACCCTCATTAATCAGTCg                                                                               >  1:88983/1‑140 (MQ=255)                                      agcGTTTGCTGCGCCTGCTACCAGCAGAGCTGGGACCAGGAGGGACAGTACTTTAACTTTCATGTTATTAACCCTCTGTTATATGCCTTTATTTGC‑TTTTTATGCCACTGCATACTGATTAACCCTCATTAATCAGTCgg                                                                              <  1:314711/140‑1 (MQ=255)                                      agcGTTTGCTGCGCCTGCTACCAGCAGAGCTGGGACCAGGAGGGACAGTACTTTAACTTTCATGTTATTAACCCTCTGTTATATGCCTTTATTTGC‑TTTTTATGCCACTGCATACTGATTAACCCTCATTAATCAGTCgg                                                                              <  2:207846/140‑1 (MQ=255)                                         gTTTGCTGCGCCTGCTACCAGCAGAGCTGGGACCAGGAGGGACAGTACTTTAACTTTCATGTTATTAACCCTCTGTTATATGCCTTTATTTGC‑TTTTTATGCCACTGCATACTGATTAACCCTCATTAATCAGTCGGCaa                                                                           >  2:239928/1‑140 (MQ=255)                                          tttGCTGCGCCTGCTACCAGCAGAGCTGGGACCAGGAGGGACAGTACTTTAACTTTCATGTTATTAACCCTCTGTTATATGCCTTTATTTGC‑TTTTTATGCCACTGCATACTGATTAACCCTCATTAATCAGTCGGCAAg                                                                          >  2:207425/1‑140 (MQ=255)                                           ttGCTGCGCCTGCTACCAGCAGAGCTGGGACCAGGAGGGACAGTACTTTAACTTTCATGTTATTAACCCTCTGTTATATGCCTTTATTTGC‑TTTTTATGCCACTGCATACTGATTAACCCTCATTAATCAGTCGGCAAGt                                                                         <  1:207425/140‑1 (MQ=255)                                                                 agCTGGGACCAGGAGGGACAGTACTTTAACTTTCATGTTATTAACCCTCTGTTATATGCCTTTATTTGC‑TTTTTATGCCACTGCATACTGATTAACCCTCATTAATCAGTCGGCAAGTCCATTCTCCCCAAAAATGCAGa                                                   >  1:136276/1‑140 (MQ=255)                                                                          cAGGAGGGACAGTACTTTAACTTTCATGTTATTAACCCTCTGTTATATGCCTTTATTTGC‑TTTTTATGCCACTGCATACTGATTAACCCTCATTAATCAGTCGGCAAGTCCATTCTCCCCAAAAATGCAGAATAATCCaa                                          <  2:6281/140‑1 (MQ=255)                                                                                           tAACTTTCATGTTATTAACCCTCTGTTATATGCCTTTATTTGC‑TTTTTATGCCACTGCATACTGATTAACCCTCATTAATCAGTCGGCAAGTCCATTCTCCCCAAAAATGCAGAATAATCCAACACGAATATGATACTaa                         >  2:213072/1‑140 (MQ=255)                                                                                           tAACTTTCATGTTATTAACCCTCTGTTATATGCCTTTATTTGC‑TTTTTATGCCACTGCATACTGATTAACCCTCATTAATCAGTCGGCAAGTCCATTCTCCCCAAAAATGCAGAATAATCCAACACGAATATGATACTaa                         >  2:196719/1‑140 (MQ=255)                                                                                            aaCTTTCATGTTATTAACCCTCTGTTATATGCCTTTATTTGC‑TTTTTATGCCACTGCATACTGATTAACCCTCATTAATCAGTCGGCAAGTCCATTCTCCCCAAAAATGCAGAATAATCCAACACGAATATGATACTaaa                        <  1:213072/140‑1 (MQ=255)                                                                                            aaCTTTCATGTTATTAACCCTCTGTTATATGCCTTTATTTGC‑TTTTTATGCCACTGCATACTGATTAACCCTCATTAATCAGTCGGCAAGTCCATTCTCCCCAAAAATGCAGAATAATCCAACACGAATATGATACTaaa                        <  1:196719/140‑1 (MQ=255)                                                                                                                 ctGTTATATGCCTTTATTTGC‑TTTTTATGCCACTGCATACTGATTAACCCTCATTAATCAGTCGGCAAGTCCATTCTCCCCAAAAATGCAGAATAATCCAACACGAATATGATACTAAAACTTTTAAg               >  2:242787/1‑128 (MQ=255)                                                                                                                 ctGTTATATGCCTTTATTTGC‑TTTTTATGCCACTGCATACTGATTAACCCTCATTAATCAGTCGGCAAGTCCATTCTCCCCAAAAATGCAGAATAATCCAACACGAATATGATACTAAAACTTTTAAg               <  1:242787/128‑1 (MQ=255)                                                                                                                  tGTTATATGCCTTTATTTGC‑TTTTTATGCCACTGCATACTGATTAACCCTCATTAATCAGTCGGCAAGTCCATTCTCCCCAAAAATGCAGAATAATCCAACACGAATATGATACTAAAACTTTTAAGATGTTTCATTTAt  <  2:88983/140‑1 (MQ=255)                                                                                                                                      |                                                                                                                         GATCTAATTTGTTGCCGTCTTTGTTGTAAACTTCAGCAGCGTTTGCTGCGCCTGCTACCAGCAGAGCTGGGACCAGGAGGGACAGTACTTTAACTTTCATGTTATTAACCCTCTGTTATATGCCTTTATTTGCTTTTTTATGCCACTGCATACTGATTAACCCTCATTAATCAGTCGGCAAGTCCATTCTCCCCAAAAATGCAGAATAATCCAACACGAATATGATACTAAAACTTTTAAGATGTTTCATTTAT  >  NC_000913/2312650‑2312903

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑