Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	112,064	(G)5→6	coding (536/744 nt)	zapD ←	cell division factor ZapD

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	112,059	1	.	G	100.0%	27.6 / NA	9	F181L (TTC→CTC)	zapD	cell division factor ZapD
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base G (3/6);  total (3/6)

ATAAAACGAATGGCAAAACGGCTCTTATGTCCGGAAATTTGCGGATAAAGCTGTGAATCGAGCGACAGATTCAGGCGCAGCAAGTCGGCATCGCCACCGTTATCCTGATAAAAACCATTCAGGCTGGTTTGTTTACGGAA‑GGGGGCCGACTGGCGAATTAAATCCAGCACCATGGTAAGTGCCTGGGTGAGCGGGTTCAGGCTGGCAATCCAGGTTTCTACCTGGCTGTCGCGCTGCGCCTGGGGT  >  NC_000913/111920‑112165                                                                                                                                             |                                                                                                           aTAAAACGAATGGCAAAACGGCTCTTATGTACGGAAATTTGCGGATAAAGCTGTGAATCGAGCGACAGATTCAGGCGCAGCAAGTCGGCATCGCCACCGTTATCCTGATAAAAACCATTCAGGCTGGTTTGTTTACGGaa                                                                                                             <  1:269983/140‑1 (MQ=255)    aaaCGAATGGCAAAACGGCTCTTATGTCCGGAAATTTGCGGATAAAGCTGTGAATCGAGCGACAGATTCAGGCGCAGCAAGTCGGCATCGCCACCGTTATCCTGATAAAAACCATTCAGGCTGGTTTGTTTACGGAA‑ggg                                                                                                         >  1:216565/1‑140 (MQ=255)                        ctTATGTCCGGAAATTTGCGGATAAAGCTGTGAATCGAGCGACAGATTCAGGCGCAGCAAGTCGGCATCGCCACCGTTATCCTGATAAAAACCATTCAGGCTGGTTTGTTTACGGAAGGGGGGCCGACTGGCGAAtt                                                                                         >  1:344278/1‑137 (MQ=255)                        ctTATGTCCGGAAATTTGCGGATAAAGCTGTGAATCGAGCGACAGATTCAGGCGCAGCAAGTCGGCATCGCCACCGTTATCCTGATAAAAACCATTCAGGCTGGTTTGTTTACGGAAGGGGGGCCGACTGGCGAAtt                                                                                         <  2:344278/137‑1 (MQ=255)                                  gAAATTTGCGGATAAAGCTGTGAATCGAGCGACAGATTCAGGCGCAGCAAGTCGGCATCGCCACCGTTATCCTGACAAAAACCATTCAGGCTGGTTTGTTTACGGAAGGGGGGCCGACTGGCGAATTAAATCCAGCACCa                                                                            <  2:243002/140‑1 (MQ=255)                                                     gtgAATCGAGCGACAGATTCAGGCGCAGCAAGTCGGCATCGCCACCGTTATCCTGATAAAAACCATTCAGGCTGGTTTGTTTACGGAAGGGGGGCCGACTGGCGAATTAAATCCAGCACCATGGTAAGTGCCTGGGTGAg                                                         <  2:284164/140‑1 (MQ=255)                                                           cGAGCGTCAGATTCAGGCGCAGCAAGTCGGCATCGCCACCGTTATCCTGATAAAAACCATTCAGGCTGGTTTGTTTACGGAA‑GGGGGCCGACTGGCGAATTAAATCCAGCACCATGGTAAGTGCCTGGGTGAGCGGGTTc                                                  <  2:309203/140‑1 (MQ=255)                                                                         aGGCGCAGCAAGTCGGCATCGCCACCGTTATCCTGATAAAAACCATTCAGGCTGGTTTGTTTACGGAAGGGGGGCCGACTGGCGAATTAAATCCAGCACCATGGTAAGTGCCTGGGTGAGCGGGTTCAGGCTGGCAATcc                                     <  2:42804/140‑1 (MQ=255)                                                                               agcaAGTCGGCATCGCCACCGTTATCCTGATAAAAACCATTCAGGCTGGTTTGTTTACGGAAGGGGGGCCGACTGGCGAATTAAATCCAGCACCATGGTAAGTGCCTGGGTGAGCGGGTTCAGGCTGGCAATCCAGGttt                               <  1:266030/140‑1 (MQ=255)                                                                                               cACCGTTATCCTGATAAAAACCATTCAGGCTGGTTTGTTTACGGAAGGGGGGCCGACTGGCGAATTAAATCCAGCACCATGGTAAGTGCCTGGGTGAGCGGGTTCAGGCTGGCAATCCAGGTTTCTACCTGGCTGTtgcg               >  1:2584/1‑140 (MQ=255)                                                                                                      aTCCTGATAAAAACCATTCAGGCTGGTTTGTTTACGGAAGGGGGGCCGACTGGCGAATTAAATCCAGCACCATGGTAAGTGCCTGGGTGAGCGGGTTCAGGCTGGCAATCCAGGTTTCTACCTGGCTGTTGCGCTGCGcc        <  2:2584/140‑1 (MQ=255)                                                                                                            aTAAAAACCATTCAGGCTGGTTTGTTTACGGAAGGGGGGCCGACTGGCGAATTAAATCCAGCACCATGGTAAGTGCCTGGGTGAGCGGGTTCAGGCTGGCAATCCAGGTTTCTACCTGGCTGTCGCGCTGCGCCTGGGGt  >  1:205047/1‑140 (MQ=255)                                                                                                                                             |                                                                                                           ATAAAACGAATGGCAAAACGGCTCTTATGTCCGGAAATTTGCGGATAAAGCTGTGAATCGAGCGACAGATTCAGGCGCAGCAAGTCGGCATCGCCACCGTTATCCTGATAAAAACCATTCAGGCTGGTTTGTTTACGGAA‑GGGGGCCGACTGGCGAATTAAATCCAGCACCATGGTAAGTGCCTGGGTGAGCGGGTTCAGGCTGGCAATCCAGGTTTCTACCTGGCTGTCGCGCTGCGCCTGGGGT  >  NC_000913/111920‑112165

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑