Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	29,113	C→T	A247V (GCG→GTG)	dapB →	4‑hydroxy‑tetrahydrodipicolinate reductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	29,113	0	C	T	100.0%	76.2 / NA	23	A247V (GCG→GTG)	dapB	4‑hydroxy‑tetrahydrodipicolinate reductase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (12/11);  total (12/11)

TGGCACCATTGGTTTTGCCACCGTGCGTGCAGGTGACATCGTTGGTGAACATACCGCGATGTTTGCCGATATTGGCGAGCGTCTGGAGATCACCCATAAGGCGTCCAGCCGTATGACATTTGCTAACGGCGCGGTAAGATCGGCTTTGTGGTTGAGTGGTAAGGAAAGCGGTCTTTTTGATATGCGAGATGTACTTGATCTCAATAATTTGTAACCACAAAATATTTGTTATGGTGCAAAAATAACACATTTAATTTATTGATTATAA  >  NC_000913/28982‑29249                                                                                                                                    |                                                                                                                                         tgacACCATTGGTTTTGCCACCGTGCGTGCAGGTGACATCGTTGGTGAACATACCGCGATGTTTGCCGATATTGGCGAGCGTCTGGAGATCACCCATAAGGCGTCCAGCCGTATGACATTTGCTAACGGCGTGGTAAGAt                                                                                                                                  >  1:1805/4‑140 (MQ=255)              tttGCCACCGTGCGTGCAGGTGACATCGTTGGTGAACATACCGCGATGTTTGCCGATATTGGCGAGCGTCTGGAGATCACCCATAAGGCGTCCAGCCGTATGACATTTGCTAACGGCGTGGTAAGATCGGCTTTGTGGtt                                                                                                                     <  1:246787/140‑1 (MQ=255)              tttGCCACCGTGCGTGCAGGTGACATCGTTGGTGAACATACCGCGATGTTTGCCGATATTGGCGAGCGTCTGGAGATCACCCATAAGGCGTCCAGCCGTATGACATTTGCTAACGGCGTGGTAAGATCGGCTTTGTGGtt                                                                                                                     <  2:1805/140‑1 (MQ=255)                tGCCACCGTGCGTGCAGGTGACATCGTTGGTGAGCATACCGCGATGTTTGCCGATATTGGCGAGCGTCTGGAGATCACCCATAAGGCGTCCAGCCGTATGACATTTGCTAACGGCGTGGTAAGATCGGCTTTGTGGTTGa                                                                                                                   <  2:300857/140‑1 (MQ=255)                                ggTGACATCGTTGGTGAACATACCGCGATGTTTGCCGATATTGGCGAGCGTCTGGAGATCACCCATAAGGCGTCCAGCCGTATGACATTTGCTAACGGCGTGGTAAGATCGGCTTTGTGGTTGAGTGGTAAGGAAAGCgg                                                                                                   <  2:45797/140‑1 (MQ=255)                                 gTGACATCGTTGGTGAACATACCGCGATGTTTGCCGATATTGGCGAGCGTCTGGAGATCACCCATAAGGCGTCCAGCCGTATGACATTTGCTAACGGCGTGGTAAGATCGGCTTTGTGGTTGa                                                                                                                   >  1:254204/1‑123 (MQ=255)                                 gTGACATCGTTGGTGAACATACCGCGATGTTTGCCGATATTGGCGAGCGTCTGGAGATCACCCATAAGGCGTCCAGCCGTATGACATTTGCTAACGGCGTGGTAAGATCGGCTTTGTGGTTGa                                                                                                                   <  2:254204/123‑1 (MQ=255)                                    aCATCGTTGGTGAACATACCGCGATGTTTGCCGATATTGGCGAGCGTCTGGAGATCACCCATAAGGCGTCCAGCCGTATGACATTTGCTAACGGCGTGGTAAGATCGGCTTTGTGGTTGAGTGGTAAGGAAAGc                                                                                                     >  2:68976/1‑134 (MQ=255)                                    aCATCGTTGGTGAACATACCGCGATGTTTGCCGATATTGGCGAGCGTCTGGAGATCACCCATAAGGCGTCCAGCCGTATGACATTTGCTAACGGCGTGGTAAGATCGGCTTTGTGGTTGAGTGGTAAGGAAAGc                                                                                                     <  1:68976/134‑1 (MQ=255)                                          ttGGTGAACATACCGCGATGTTTGCCGATATTGGCGAGCGTCTGGAGATCACCCATAAGGCGTCCAGCCGTATGACATTTGCTAACGGCGTGGTAAGATCGGCTTTGTGGTTGAGTGGTAAGGAAAGCGGTCTTTTTGat                                                                                         <  1:254892/140‑1 (MQ=255)                                             gTGAACATACCGCGATGTTTGCCGATATTGGCGAGCGTCTGGAGATCACCCATAAGGCGTCCAGCCGTATGACATTTGCTAACGGCGTGGTAAGATCGGCTTTGTGGTTGAGTGGTAAGGAAAGCGGTCTTTTTGATATg                                                                                      <  1:64953/140‑1 (MQ=255)                                               gAACATACCGCGATGTTTGCCGATATTGGCGAGCGTCTGGAGATCACCCATAAGGCGTCCAGCCGTATGACATTTGCTAACGGCGTGGTAAGATCGGCTTTGTGGTTGAGTGGTAAGGAAAGCGGTCTTTTTGATATGCg                                                                                    <  2:126347/140‑1 (MQ=255)                                                    tACCGCGATGTTTGCCGATATTGGCGAGCGTCTGGAGATCACCCATAAGGCGTCCAGCCGTATGACATTTGCTAACGGCGTGGTAAGATCGGCTTTGTGGTTGAGTGGTAAGGAAAGCGGTCTTTTTGATATGCGAGATg                                                                               >  2:259718/1‑140 (MQ=255)                                                     aCCGCGATGTTTGCCGATATTGGCGAGCGTCTGGAGATCACCCATAAGGCGTCCAGCCGTATGACATTTGCTAACGGCGTGGTAAGATCGGCTTTGTGGTTGAGTGGTAAGGAAAGCGGTCTTTTTGATATGCGAGATGt                                                                              >  1:184594/1‑140 (MQ=255)                                                              tttGCCGATATTGGCGAGCGTCTGGAGATCACCCATAAGGCGTCCAGCCGTATGACATTTGCTAACGGCGTGGTAAGATCGGCTTTGTGGGTGAGTGGTAAGGAAAGCGGTCTTTTTGATATGCGAGATGTACTTGAtct                                                                     >  2:71789/1‑140 (MQ=255)                                                                                           cACCCATAAGGCGTCCAGCCGTATGACATTTGCTAACGGCGTGGTTAGATTGGGTTTGTGGGTTAGCGGTAAGGAAAGGGGGCTTTTTGGAATGCGGGATTTATTTGATCTCCATTAATTTTAAACACCAAATATTTGtt                                        >  2:98492/1‑140 (MQ=255)                                                                                           cACCCATAAGGCGTCCAGCCGTATGACATTTGCTAACGGCGTGGTAAGATCGGCTTTGTGGTTGAGTGGTAAGGAAAGCGGTCTTTTTGATATGCGAGATGTACTTGATCTCAATAATTTGTAACCACAAAATATTTGtt                                        >  2:166730/1‑140 (MQ=255)                                                                                                aTAAGGCGTCCAGCCGTACGACATTTGCTAACGGCGTGGTAAGATCGGCTTTGTGGTTGAGTGGGAAGGAAAGCGGTCTTTTTGATATGCGAGATGTACTTGATCTCAATAATTTGTAACCACAAAATATTTGTTATGGt                                   >  2:129905/1‑140 (MQ=255)                                                                                                   aGGCGTCCAGCCGTATGACATTTGCTAACGGCGTGGTAAGATCGGCTTTGTGGTTGAGTGGTAAGGAAAGCGGTCTTTTTGATATGCGAGATGTACTTGATCTCAATAATTTGTAACCACAAAATATTTGTTATGGTGCa                                <  1:259718/140‑1 (MQ=255)                                                                                                   aGGCGTCCAGCCGTATGACATTTGCTAACGGCGTGGGAAGATCGGCTTTGTGGTTGAGTGGTAAGGAAAGCGGTCTTTTTGATATGCGAGATGTACTTGATCTCAATAATTTGTAACCACAAAATATTTGTTATGGTGCa                                >  1:74242/1‑140 (MQ=255)                                                                                                                        ttGCTAACGGCGTGGTAAGATCGGCTTTGTGGTTGAGTGGTAAGGAAAGCGGTCTTTTTGATATGCGAGATGTACTTGATCTCAATAATTTGTAACCACAAAATATTTGTTATGGTGCAAAAATAACACATTTAATTTAt           >  1:30580/1‑140 (MQ=255)                                                                                                                        ttGCTAACGGCGTGGTAAGATCGGCTTTGTGGTTGAGTGGTAAGGAAAGCGGTCTTTTTGATATGCGAGATGTACTTGATCTCAATAATTTGTAACCACAAAATATTTGTTATGGTGCAAAAATAACACATTTAATTTAt           <  1:166730/140‑1 (MQ=255)                                                                                                                               cGGCGTGGTAAGATCGGCTTTGTGGTTGAGTGGTAAGGAAAGCGGGCTTTTTGATATGCGAGATGTACTTGATCTCAATAATTTGTAACCACAAAATATTTGTTATGGTGCAAAAATAACACATTTAATTTATTGATtat    >  2:296127/1‑140 (MQ=255)                                                                                                                                 gcgtGGTAAGATAGGCCTTGAGGTTGAGGGGTGAGGAAAGCGGGCTTTTTTGTATGCGAGATGTACTTGATCTCAATAATTTTTAACCACAAAATATTTGTTTTGGTGCGAAAATAAAACATTTAATTTATTGATTATaa  >  2:265864/1‑140 (MQ=255)                                                                                                                                    |                                                                                                                                         TGGCACCATTGGTTTTGCCACCGTGCGTGCAGGTGACATCGTTGGTGAACATACCGCGATGTTTGCCGATATTGGCGAGCGTCTGGAGATCACCCATAAGGCGTCCAGCCGTATGACATTTGCTAACGGCGCGGTAAGATCGGCTTTGTGGTTGAGTGGTAAGGAAAGCGGTCTTTTTGATATGCGAGATGTACTTGATCTCAATAATTTGTAACCACAAAATATTTGTTATGGTGCAAAAATAACACATTTAATTTATTGATTATAA  >  NC_000913/28982‑29249

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑