Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,334,717	T→C	intergenic (+36/‑192)	ispB → / → sfsB	all‑trans‑octaprenyl‑diphosphate synthase/putative transcriptional regulator SfsB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,334,717	0	T	C	100.0%	72.0 / NA	21	intergenic (+36/‑192)	ispB/sfsB	all‑trans‑octaprenyl‑diphosphate synthase/putative transcriptional regulator SfsB
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (9/12);  total (9/12)

TCGCAGCGTTACAGGTGCTCCCGGACACCCCTTGGCGAGAAGCACTCATCGGCCTCGCGCACATCGCTGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCAGCGCGCGGGATGGTTCCAGTAAGTTCCATAAAAACACTTATTCAGCTCTATAACACCTGAAAAATAGGGATGTGCATCTTTATATATTCTGAATATTCACACTCTTTACAGGAACTTTTTAGAGCAATAGGCCATCAGGAGTATAGTGA  >  NC_000913/3334596‑3334852                                                                                                                          |                                                                                                                                        tCGCAGCGTTACAGGTGCTCCCGGACACCCCTTGGCGAGAAGCACTCATCGGCCTCGCGCACATCGCTGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCAGCGCGCGGGATGGCTCCAGTAAGTTCCATaaa                                                                                                                       <  2:159129/140‑1 (MQ=255)           aCAGGTGCTCCCGGACACCCCTTGGCGAGAAGCACTCATCGGCCTCGCGCACATCGCTGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCAGCGCGCGGGATGGCTCCAGTAAGTTCCATAAAAACACTTAtt                                                                                                             <  2:220271/140‑1 (MQ=255)               gTGCTCCCGGACACCCCTTGGCGAGAAGCACTCATCGGCCTCGCGCACATCGCTGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCAGCGCGCGGGATGGCTCCAGTAAGTTCCATAAAAACACTTATTCAGc                                                                                                         >  1:188108/1‑140 (MQ=255)                 gCTCCCGGACACCCCTTGGCGAGAAGCACTCATCGGCCTCGCGCACATCGCTGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCAGCGCGCGGGATGGCTCCAGTAAGTTCCATAAAAACACTTATTCAGctc                                                                                                       >  1:186852/1‑140 (MQ=255)                                      agaAGCACTCATCGGCCTCGCGCACATCGCTGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCAGCGCGCGGGATGGCTCCAGTAAGTTCCATAAAAACACTTATTCAGCTCTATAACACCTGAAAAATAggg                                                                                  <  2:188108/140‑1 (MQ=255)                                           cACTCATCGGCCTCGCGCACATCGCTGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCAGCGCGCGGGATGGCTCCAGTAAGTTCCATAAAAACACTTATTCAGCTCTATAACACCTGAAAAATAGGGAtgtg                                                                             <  2:340630/140‑1 (MQ=255)                                                        cgcgCACATCGCTGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCAGCGCGCTGGATGGCTCCAGTAAGTTCCATAAAAACACTTATTCAGCTCTATAACACCTGAAAAATAGGGAtgtg                                                                             >  2:7493/1‑127 (MQ=255)                                                        cgcgCACATCGCTGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCAGCGCGCTGGATGGCTCCAGTAAGTTCCATAAAAACACTTATTCAGCTCTATAACACCTGAAAAATAGGGAtgtg                                                                             <  1:7493/127‑1 (MQ=255)                                                        cgcgCACATCGCTGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCAGCGCGCGGGATGGCTCCAGTAAGTTCCATAAAAACACTTATTCAGCTCTATAACACCTGAAAAATAGGGATGTGCATc                                                                         >  1:263529/1‑131 (MQ=255)                                                        cgcgCACATCGCTGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCAGCGCGCGGGATGGCTCCAGTAAGTTCCATAAAAACACTTATTCAGCTCTATAACACCTGAAAAATAGGGATGTGCATc                                                                         <  2:263529/131‑1 (MQ=255)                                                            cacaTCGCTGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCAGCGCGCGGGATGGCTCCAGTAAGTTCCATAAAAACACTTATTCAGCTCTATAACACCTGAAAAATAGGGATGTGCATCTTTATATATTCTg                                                            <  2:196505/140‑1 (MQ=255)                                                                      ttCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCAGCGCGCGGGATGGCTCCAGTAAGTTCCATAAAAACACTTATTCAGCTCTATAACACCTGAAAAATAGGGATGTGCATCTTTATATATTCTGAATATTcaca                                                  <  2:186852/140‑1 (MQ=255)                                                                           cgcgATCGTTAATCCCCTCCCCTCATCCCGCGCAGCGCGCGGGATGGCTCCAGTAAGTTCCATAAAAACACTTATTCAGCTCTATAACACCTGAAAAATAGGGATGTGCATCTTTATATATTCTGAATATTCACACTCtt                                             >  2:252638/1‑140 (MQ=255)                                                                           cgcgATCGTTAATCCCCTCCCCTCATCCCGCGCAGCGCGCGGGATGGCTCCAGTAAGTTCCATAAAAACACTTATTCAGCTCTATAACACCTGAAAAATAGGGATGTGCATCTTTATATATTCTGAATATTCACACTCtt                                             >  2:31952/1‑140 (MQ=255)                                                                                           ctcccctcATCCCGCGCAGCGCGCGGGATGGCTCCAGTAAGTTCCATAAAAACACTTATTCAGCTCTATAACACCTGAAAAATAGGGATGTGCATCTTTATATATTCTGAATATTCACACTCTTTACAGGAACTTTTTag                             >  1:143794/1‑140 (MQ=255)                                                                                             cccctcATCCCGCGCAGCGCGCGGGATGGCTCCAGTAAGTTCCATAAAAACACTTATTCAGCTCTATAACACCTGAAAAATAGGGATGTGCATCTTTATATATTCTGAATATTCACACTCTTTACAGGAACTTTTTagag                           >  2:279405/1‑140 (MQ=255)                                                                                                ctcATCCCGCGCAGCGCGCGGGATGGCTCCAGTAAGTTCCATAAAAACACTTATTCAGCTCTATAACACCTGAAAAATAGGGATGTGCATCTTTATATATTCTGAATATTCACACTCTTTACAGGAACTTTTTAGAGCaa                        >  1:161333/1‑140 (MQ=255)                                                                                                      ccGCGCAGCGCGCGGGATGGCTCCAGTAAGTTCCATAAAAACACTTATTCAGCTCTATAACACCTGAAAAATAGGGATGTGCATCTTTATATATTCTGAATATTCACACTCTTTACAGGAACTTTTTAGAGCAATAGGcc                  <  1:31952/140‑1 (MQ=255)                                                                                                      ccGCGCAGCGCGCGGGATGGCTCCAGTAAGTTCCATAAAAACACTTATTCAGCTCTATAACACCTGAAAAATAGGGATGTGCATCTTTATATATTCTGAATATTCACACTCTTTACAGGAACTTTTTAGAGCAATAGGcc                  <  1:252638/140‑1 (MQ=255)                                                                                                        gcgcAGCGCGCGGGATGGCTCCAGTAAGTTCCATAAAAACACTTATTCAGCTCTATAACACCTGAAAAATAGGGATGTGCATCTTTATATATTCTGAATATTCACACTCTTTACAGGAACTTTTTAGAGCAATAGGCCAt                <  1:167788/140‑1 (MQ=255)                                                                                                                      aTGGCTCCAGTAAGTTCCATAAAAACACTTATTCAGCTCTATAACACCTGAAAAATAGGGATGTGCATCTTTATATATTCTGAATATTCACACTCTTTACAGGAACTTTTTAGAGCAATAGGCCATCAGGAGTATAGTGa  <  2:143794/140‑1 (MQ=255)                                                                                                                          |                                                                                                                                        TCGCAGCGTTACAGGTGCTCCCGGACACCCCTTGGCGAGAAGCACTCATCGGCCTCGCGCACATCGCTGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCAGCGCGCGGGATGGTTCCAGTAAGTTCCATAAAAACACTTATTCAGCTCTATAACACCTGAAAAATAGGGATGTGCATCTTTATATATTCTGAATATTCACACTCTTTACAGGAACTTTTTAGAGCAATAGGCCATCAGGAGTATAGTGA  >  NC_000913/3334596‑3334852

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑