Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,336,470	G→A	P9S (CCA→TCA)	murA ←	UDP‑N‑acetylglucosamine 1‑carboxyvinyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,336,470	0	G	A	96.5%	87.8 / ‑3.8	29	P9S (CCA→TCA)	murA	UDP‑N‑acetylglucosamine 1‑carboxyvinyltransferase
Reads supporting (aligned to +/- strand):  ref base G (1/0);  new base A (14/14);  total (15/14)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.15e-01

TTTCAGTTTCGGGACGTTCTGGATCTCTACCGGTTCTTCCGCCAGTAGTGCGGCAAAAAGGATAGGCAGAGCAGCATTTTTAGCGCCGGAAATTGTGACTTCGCCCTGGAGCTTCGTTGGCCCCTGAACACGAAATTTATCCATTTAGTTTGTTCTCAGTTAACAATTCATATCCGCTACCGGCGAATCGCCCATAGCTCAAAAGCCGTTCAGTTTGCGATCGCGCGCCCACTCCGCAGGGGTATACGCTTTGAT  >  NC_000913/3336351‑3336605                                                                                                                        |                                                                                                                                        tttCAGTTTCGGGACGTTCTGGATCTCTACCGGTTCTTCCGCCAGTAGTGCGGCAAAAAGGATAGGCAGAGCAGCATTTTTAGCGCCGGAAATTGTGACTTCGCCCTGGAGCTTCGTTGACCCCTGAACACGAAATTTAt                                                                                                                     >  1:325964/1‑140 (MQ=255)   tCAGTTTCGGGACGTTCTGGATCTCTACCGGTTCTTCCGCCAGTAGTGCGGCAAAAAGGATAGGCAGAGCAGCATTTTTAGCGCCGGAAATTGTGACTTCGCCCTGGAGCTTCGTTGACCCCTGAACACGAAATTTATcc                                                                                                                   >  2:277315/1‑140 (MQ=255)                 ttCTGGATCTCTACCGGTTCTTCCGCCAGTAGTGCGGCAAAAAGGATAGGCAGAGCAGCATTTTTAGCGCCGGAAATTGTGACTTCGCCCTGGAGCTTCGTTGACCCCTGAACACGAAATTTATCCATTTAGTTTGTtct                                                                                                     >  2:61821/1‑140 (MQ=255)                            tACCGGTTCTTCCGCCAGTAGTGCGGCAAAAAGGATAGGCAGAGCAGCATTTTTAGCGCCGGAAATTGTGACTTCGCCCTGGAGCTTCGTTGACCCCTGAACACGAAATTTATCCATTTAGt                                                                                                            <  2:340013/122‑1 (MQ=255)                            tACCGGTTCTTCCGCCAGTAGTGCGGCAAAAAGGATAGGCAGAGCAGCATTTTTAGCGCCGGAAATTGTGACTTCGCCCTGGAGCTTCGTTGACCCCTGAACACGAAATTTATCCATTTAGt                                                                                                            >  1:340013/1‑122 (MQ=255)                            tACCGGTTCTTCCGCCAGTAGTGCGGCAAAAAGGATAGGCAGAGCAGCATTTTTAGCGCCGGAAATTGTGACTTCGCCCTGGAGCTTCGTTGACCCCTGAACACGAAATTTATCCATTTAGTTTGTTCTCAGTTaa                                                                                              >  2:99810/1‑136 (MQ=255)                            tACCGGTTCTTCCGCCAGTAGTGCGGCAAAAAGGATAGGCAGAGCAGCATTTTTAGCGCCGGAAATTGTGACTTCGCCCTGGAGCTTCGTTGACCCCTGAACACGAAATTTATCCATTTAGTTTGTTCTCAGTTaa                                                                                              <  1:99810/136‑1 (MQ=255)                            tACCGGTTCTTCCGCCAGTAGTGCGGCAAAAAGGATAGGCAGAGCAGCATTTTTAGCGCCGGAAATTGTGACTTCGCCCTGGAGCTTCGTTGACCCCTGAACACGAAATTTATCCATTTAGTTTGTTCTCAGTTAACAAt                                                                                          >  1:246905/1‑140 (MQ=255)                            tACCGGTTCTTCCGCCAGTAGTGCGGCAAAAAGGATAGGCAGAGCAGCATTTTTAGCGCCGGAAATTGTGACTTCGCCCTGGAGCTTCGTTGACCCCTGAACACGAAATTTATCCATTTAGTTTGTTCTCAGTTAACAAt                                                                                          >  1:302130/1‑140 (MQ=255)                                 gTTCTTCCGCCAGTAGTGCGGCAAAAAGGATAGGCAGAGCAGCATTTTTAGCGCCGGAAATTGTGACTTCGCCCTGGAGCTTCGTTGACCCCTGAACACGAAATTTATCCATTTAGTTTGTTCTCAGTTAACAATTCata                                                                                     <  2:325964/140‑1 (MQ=255)                                             gtagtGCGGCAAAAAGGATAGGCAGAGCAGCATTTTTAGCGCCGGAAATTGTGACTTCGCCCTGGAGCTTCGTTGACCCCTGAACACGAAATTTATCCATTTAGTTTGTTCTCAGTTAACAATTCATATCCGCTACCGGc                                                                         <  1:6666/140‑1 (MQ=255)                                              tagtGCGGCAAAAAGGATAGGCAGAGCAGCATTTTTAGCGCCGGAAATTGTGACTTCGCCCTGGAGCTTCGTTGACCCCTGAACACGAAATTTATCCATTTAGTTTGTTCTCAGTTAACAATTCATATCCGCTACCGGCg                                                                        >  1:20914/1‑140 (MQ=255)                                              tagtGCGGCAAAAAGGATAGGCAGAGCAGCATTTTTAGCGCCGGAAATTGTGACTTCGCCCTGGAGCTTCGTTGACCCCTGAACACGAAATTTATCCATTTAGTTTGTTCTCAGTTAACAATTCATATCCGCTACCGGCg                                                                        >  1:338124/1‑140 (MQ=255)                                                       aaaaaGGATAGGCAGAGCAGCATTTTTAGCGCCGGAAATTGTGACTTCGCCCTGGAGCTTCGTTGACCCCTGAACACGAAATTTATCCATTTAGTTTGTTCTCAGTTAACAATTCATATCCGCTACCGGCGAATCGCCCa                                                               >  1:21393/1‑140 (MQ=255)                                                                     gagCAGCATTTTTAGCGCCGGAAATTGTGACTTCGCCCTGGAGCTTCGTTGACCCCTGAACACGAAATTTATCCATTTAGTTTGTTCTCAGTTAACAATTCATATCCGCTACCGGCGAATCGCCCATAGCTCAAAAGCCg                                                 <  1:277315/140‑1 (MQ=255)                                                                       gcagcaTTTTTAGCGCCGGAAATTGTGACTTCGCCCTGGAGCTTCGTTGACCCCTGAACACGAAATTTATCCATTTAGTTTGTTCTCAGTTAACAATTCATATCCGCTACCGGCGAATCGCCCATAGCTCAAAAGCCGtt                                               <  1:304973/140‑1 (MQ=255)                                                                       gcagcaTTTTTAGCGCCGGAAATTGTGACTTCGCCCTGGAGCTTCGTTGACCCCTGAACACGAAATTTATCCATTTAGTTTGTTCTCAGTTAACAATTCATATCCGCTACCGGCGAATCGCCCATAGCTCAAAAGCCGtt                                               <  1:61821/140‑1 (MQ=255)                                                                       gcagcaTTTTTAGCACCGGAAATTGTGACTTCGCCCTGGAGCTTCGTTGACCCCTGAACACGAAATTTATCCATTTAGTTTGTTCTCAGTTAACAATTCATATCCGCTACCGGCGAATCGCCCATAGCTCAAAAGCCGtt                                               >  1:160733/1‑140 (MQ=255)                                                                           caTTTTTAGCGCCGGAAATTGTGACTTCGCCCTGGAGCTTCGTTGACCCCTGAACACGAAATTTATCCATTTAGTTTGTTCTCAGTTAACAATTCATATCCGCTACCGGCGAATCGCCCATAGCTCAAAAGCCGTTCAGt                                           >  2:343873/1‑140 (MQ=255)                                                                                          aaaTTGTGACTTCGCCCTGGAGCTTCGTTGACCCCTGAACACGAAATTTATCCATTTAGTTTGTTCTCAGTTAACAATTCATATCCGCTACCGGCGAATCGCCCATAGCTCAAAAGCCGTTCAGTTTGCGATCGCGCGcc                            <  2:20914/140‑1 (MQ=255)                                                                                            aTTGTGACTTCGCCCTGGAGCTTCGTTGACCCCTGAACACGAAATTTATCCATTTAGTTTGTTCTCAGTTAACAATTCATATCCGCTACCGGCGAATCGCCCATAGCTCAAAAGCCGTTCAGTTTGCGATCGCGCGCCCa                          <  1:343873/140‑1 (MQ=255)                                                                                                tgACTTCGCCCTGGAGCTTCGTT‑GACCCTGAACACGAAATTTATCCATTTAGTTTGTTCTCAGTTAACAATTCATATCCGCTACCGGCGAATCGCCCATAGCTCAAAAGCCGTTCAGTTTGCGATCGCGCGCCCACTCCg                     >  1:79573/1‑140 (MQ=255)                                                                                                 gACTTCGCCCTGGAGCTTCGTTGACCCCTGAACACGAAATTTATCCATTTAGTTTGTTCTCAGTTAACTATTCATATCCGCTACCGGCGAATCGCCCATAGCTCAAAAGCCGTTCAGTTTGCGATCGCGCGCCCACTCCg                     <  2:246905/140‑1 (MQ=255)                                                                                                   cTTCGCCCTGGAGCTTCGTTGACCCCTGAACACGAAATTTATCCATTTAGTTTGTTCTCAGTTAACAATTCATATCCGCTACCGGCGAATCGCCCATAGCTCAAAAGCCGTTCAGTTTGCGATCGCGCGCCCACTCCGCa                   <  1:239047/140‑1 (MQ=255)                                                                                                   cTTCGCCCTGGAGCTTCGTTGACCCCTGAACACGAAATTTATCCATTTAGTTTGTTCTCAGTTAACAATTCATATCCGCTACCGGCGAATCGCCCATAGCTCAAAAGCCGTTCAGTTTGCGATCGCGCGCCCACTCCGCa                   <  1:216314/140‑1 (MQ=255)                                                                                                    ttCGCCCTGGAGCTTCGTTGACCCCTGAACACGAAATTTATCCATTTAGTTTGTTCTCAGTTAACAATTCATATCCGCTACCGGCGAATCGCCCATAGCTCAAAAGCCGTTCAGTTTGCGATCGCGCGCCCACTCCGCAg                  >  2:331267/1‑140 (MQ=255)                                                                                                                  tCGTTGACCCCTGAACACGAAATTTATCCATTTAGTTTGTTCTCAGTTAACAATTCATATCCGCTACCGGCGAATCGCCCATAGCTCAAAAGCCGTTCAGTTTGCGATCGCGCGCCCACTCCGCAGGGGTATACGCTTTg    >  1:90210/1‑140 (MQ=255)                                                                                                                    gTTGACCCCTGAACACGAAATTTATCCATTTAGTTTGTTCTCAGTTAACAATTCATATCCGCTACCGGCGAATCGCCCATAGCTCAAAAGCCGTTCAGTTTGCGATCGCGCGCCCACTCCGCAGGGGTATACGCTTTGAt  <  2:338124/140‑1 (MQ=255)                                                                                                                    gTTGACCCCTGAACACGAAATTTATCCATTTAGTTTGTTCTCAGTTAACAATTCATATCCGCTACCGGCGAATCGCCCATAGCTCAAAAGCCGTTCAGTTTGCGATCGCGCGCCCACTCCGCAGGGGTATACGCTTTGAt  <  2:90210/140‑1 (MQ=255)                                                                                                                        |                                                                                                                                        TTTCAGTTTCGGGACGTTCTGGATCTCTACCGGTTCTTCCGCCAGTAGTGCGGCAAAAAGGATAGGCAGAGCAGCATTTTTAGCGCCGGAAATTGTGACTTCGCCCTGGAGCTTCGTTGGCCCCTGAACACGAAATTTATCCATTTAGTTTGTTCTCAGTTAACAATTCATATCCGCTACCGGCGAATCGCCCATAGCTCAAAAGCCGTTCAGTTTGCGATCGCGCGCCCACTCCGCAGGGGTATACGCTTTGAT  >  NC_000913/3336351‑3336605

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑