Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,350,364	G→A	S32S (AGC→AGT)	elbB ←	low activity glyoxalase ElbB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,350,364	0	G	A	100.0%	54.4 / NA	17	S32S (AGC→AGT)	elbB	low activity glyoxalase ElbB
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (11/6);  total (11/6)

AGGACGGATTTCACCGCGCGTTATTCGTGCCGCTTCAATCAGCACATTGCGCGTTTCCGTCATCGCTTCGCCAGTTAAATGGTTGATAACATCAACCTGCTGCTTATCCGGTGCAAAGCAGACCGCCTGCGCACCGCTGCGTGAAATAGCTAACAACGTCAACACCGCTTCATGAATTTCAGAACCGTCATAGACGCCGCATCCGCTCAGAATTACGCCAATTTTCTTCATTGTGATCATCCTTTCGCAATTGACTGAA  >  NC_000913/3350229‑3350487                                                                                                                                        |                                                                                                                            agGACGGATTTCACCGCGCGTTATTCGTGCCGCTTCAATCAGCACATTGCGCGTTTCCGTCATCGCTTCGCCAGTTAAATGGTTGATAACATCAACCTGCTGCTTATCCGGTGCAAAGCAGACCGCCTGCGCACCACTgt                                                                                                                         >  2:279956/1‑139 (MQ=255)                   cgTTATTCGTGCCGCTTCAATCAGCACATTGCGCGTTTCCGTCATCGCTTCGCCAGTTAAATGGTTGATAACATCAACCTGCTGCTTATCCGGTGCAAAGCAGACCGCCTGCGCACCACTGCGTGAAATAGCTAACAACg                                                                                                       >  1:33185/1‑140 (MQ=255)                        ttCGTGCCGCTTCAATCAGCACATTGCGCGTTTCCGTCATCGCTTCGCCAGTTAAATGGTTGATAACATCAACCTGCTGCTTATCCGGTGCAAAGCAGACCGCCTGCGCACCACTGCGTGAAATAGCTAACAACGTCAac                                                                                                  <  1:57565/140‑1 (MQ=255)                             gCCGCTTCAATCAGCACATTGCGCGTTTCCGTCATCGCTTCGCCAGTTAAATGGTTGATAACATCAACCTGCTGCTTATCCGGTGCAAAGCAGACCGCCTGCGCACCACTGCGTGAAATAGCTAACAACGTCAACACCGc                                                                                             >  1:291147/1‑140 (MQ=255)                                             caTTGCGCGTTTCCGTCATCGCTTCGCCAGTTAAATGGTTGATAACATCAACCTGCTGCTTATCCGGTGCAAAGCAGACCGCCTGCGCACCACTGCGTGAAATAGCTAACAACGTCAACACCGCTTCATGAATTTCAGaa                                                                             <  2:291147/140‑1 (MQ=255)                                             caTTGCGCGTTTCCGTCATCGCTTCGCCAGTTAAATGGTTGATAACATCAACCTGCTGCTTATCCGGTGCAAAGCAGACCGCCTGCGCACCACTGCGTGAAATAGCTAACAACGTCAACACCGCTTCATGAATTTCAGaa                                                                             <  2:16788/140‑1 (MQ=255)                                                         ccGTCATCGCTTCGCCAGTTAAATGGTTGATAACATCAACCTGCTGCTTATCCGGTGCAAAGCAGACCGCCTGCGCACCACTGCGTGAAATAGCTAACAACGTCAACACCGCTTCATGAATTTCAGAACCGTCATAGAcg                                                                 >  2:88409/1‑140 (MQ=255)                                                                                tGGTTGATAACATCAACCTGCTGCTTATCCGGTGCAAAGCAGACCGCCTGCGCACCACTGCGTGAAATAGCTAACAACGTCAACACCGCTTCATGAATTTCAGAACCGTCATAGACGCCGCATCCGCTCAGAATTACGc                                           >  2:269912/1‑139 (MQ=255)                                                                                tGGTTGATAACATCAACCTGCTGCTTATCCGGTGCAAAGCAGACCGCCTGCGCACCACTGCGTGAAATAGCTAACAACGTCAACACCGCTTCATGAATTTCAGAACCGTCATAGACGCCGCATCCGCTCAGAATTACGc                                           <  1:269912/139‑1 (MQ=255)                                                                                             cAACCTGCTGCTTATCCGGTGCAAAGCAGACCGCCTGCGCACCACTGCGTGAAATAGCTAACAACGTCAACACCGCTTCATGAATTTCAGAACCGTCATAGACGCCGCATCCGCTCAGAATTACGCCAATTTTCTTCAtt                             >  2:195946/1‑140 (MQ=255)                                                                                             cAACCTGCTGCTTATCCGGTGCAAAGCAGACCGCCTGCGCACCACTGCGTGAAATAGCTAACAACGTCAACACCGCTTCATGAATTTCAGAACCGTCATAGACGCCGCATCCGCTCAGAATTACGCCAATTTTCTTCAtt                             >  1:189554/1‑140 (MQ=255)                                                                                               aCCTGCTGCTTATCCGGTGCAAAGCAGACCGCCTGCGCACCACTGCGTGAAATAGCTAACAACGTCAACACCGCTTCATGAATTTCAGAACCGTCATAGACGCCGCATCCGCTCAGAATTACGCCAATTTTCTTCATtgt                           >  2:336454/1‑140 (MQ=255)                                                                                                    ctgctTATCCGGTGCAAAGCAGACCGCCTGCGCACCACTGCGTGAAATAGCTAACAACGTCAACACCGCTTCATGAATTTCAGAACCGTCATAGACGCCGCATCCGCTCAGAATTACGCCAATTTTCTTCATTGTGatca                      >  1:297127/1‑140 (MQ=255)                                                                                                               gTGCAAAGCAGACCGCCTGCGCACCACTGCGTGAAATAGCTAACAACGTCAACACCGCTTCATGAATTTCAGAACCGTCATAGACGCCGCATCCGCTCAGAATTACGCCAATTTTCTTCATTGTGATCATCCTTTCGCaa           <  1:88409/140‑1 (MQ=255)                                                                                                               gTGCAAAGCAGACCGCCTGCGCACCACTGCGTGAAATAGCTAACAACGTCAACACCGCTTCATGAATTTCAGAACCGTCATAGACGCCGCATCCGCTCAGAATTACGCCAATTTTCTTCATTGTGATCATCCTTTCGCaa           >  2:51960/1‑140 (MQ=255)                                                                                                                     aGCAGACCGCCTGCGCACCACTGCGTGAAATAGCTAACAACGTCAACACCGCTTCATGAATTTCAGAACCGTCATAGACGCCGCATCCGCTCAGAATTACGCCAATTTTCTTCATTGTGATCATCCTTTCGCAATTGACt     >  1:107100/1‑140 (MQ=255)                                                                                                                        aGACCGCCTGCGCACCACTGCGTGAAATAGCTAACAACGTCAACACCGCTTCATGAATTTCAGAACCGTCATAGACGCCGCATCCGCTCAGAATTACGCCAATTTTCTTCATTGTGATCATCCTTTCGCAATTGACTGaa  <  2:297127/140‑1 (MQ=255)                                                                                                                                        |                                                                                                                            AGGACGGATTTCACCGCGCGTTATTCGTGCCGCTTCAATCAGCACATTGCGCGTTTCCGTCATCGCTTCGCCAGTTAAATGGTTGATAACATCAACCTGCTGCTTATCCGGTGCAAAGCAGACCGCCTGCGCACCGCTGCGTGAAATAGCTAACAACGTCAACACCGCTTCATGAATTTCAGAACCGTCATAGACGCCGCATCCGCTCAGAATTACGCCAATTTTCTTCATTGTGATCATCCTTTCGCAATTGACTGAA  >  NC_000913/3350229‑3350487

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑