Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,476,286	T→C	H52R (CAC→CGC)	yheO ←	putative DNA‑binding transcriptional regulator YheO

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,476,286	0	T	C	100.0%	83.3 / NA	24	H52R (CAC→CGC)	yheO	putative DNA‑binding transcriptional regulator YheO
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (14/10);  total (14/10)

TCCGCTCCCGTCATATCGTGCAGCATACGTAGCGCCAGGTCAGTAATTGGCGAACCAATCTTCCGGCCTGTATGTTCACCGTTAGCAATGCGAATGGCTGAACATTTTAGATCCTGCAAAGAGTGCAAAACGATTTCACAGTGGGAGCCAATAAGCATCGCTAACCCGTCCACCACCGCTTCGTAGGATTTCAGAATATCAAAATCGGTCTGGTCGAAAGGACGTTGATCCAGTAAATCCAACTCACTGGTTT  >  NC_000913/3476163‑3476415                                                                                                                            |                                                                                                                                  tCCGCTCCCGTCATATCGTGCAGCATACGTAGCGCCAGGTCAGTAATTGGCGAACCAATCTTCCGGCCTGTATGTTCACCGTTAGCAATGCGAATGGCTGAACATTTTAGATCCTGCAAAGAGCGCAAAACGATTTcaca                                                                                                                   <  2:64031/140‑1 (MQ=255)     cTCCCGTCATATCGTGCAGCATACGTAGCGCCAGGTCAGTAATTGGCGAACCAATCTTCCGGCCTGTATGTTCACCGTTAGCAATGCGAATGGCTGAACATTTTAGATCCTGCAAAGAGCGCAAAACGATTTCACAGTgg                                                                                                               <  1:238143/140‑1 (MQ=255)     cTCCCGTCATATCGTGCAGCATACGTAGCGCCAGGTAAGTAATTGGCGAACCAATCTTCCGGCCTGTATGTTCACCGTTAGCAATGCGAATGGCTGAACATTTTAGATCCTGCAAAGAGCGCAAAACGATTTCACAGTgg                                                                                                               >  2:48015/1‑140 (MQ=255)        ccGTCATATCGTGCAGCATACGTAGCGCCAGGTCAGTAATTGGCGAACCAATCTTCCGGCCTGTATGTTCACCGTTAGCAATGCGAATGGCTGAACATTTTAGATCCTGCAAAGAGCGCAAAACGAttt                                                                                                                       <  1:141549/129‑1 (MQ=255)        ccGTCATATCGTGCAGCATACGTAGCGCCAGGTCAGTAATTGGCGAACCAATCTTCCGGCCTGTATGTTCACCGTTAGCAATGCGAATGGCTGAACATTTTAGATCCTGCAAAGAGCGCAAAACGAttt                                                                                                                       >  2:141549/1‑129 (MQ=255)          gTCATATCGTGCAGCATACGTAGCGCCAGGTCAGTAATTGGCGAACCAATCTTCCGGCCTGTATGTTCACCGTTAGCAATGCGAATGGCTGAACATTTTAGATCCTGCAAAGAGCGCAAAACGATTTCACAGTGGGAGcc                                                                                                          >  1:73823/1‑140 (MQ=255)                  gTGCAGCATACGTAGCGCCAGGTCAGTAATTGGCGAACCAATCTTCCGGCCTGTATGTTCACCGTTAGCAATGCGAATGGCTGAACATTTTAGATCCTGCAAAGAGCGCAAAACGATTTCACAGTGGGAGCCAATAAGCa                                                                                                  >  1:52084/1‑140 (MQ=255)                        caTACGTAGCGCCAGGTCAGTAATTGGCGAACCAATCTTCCGGCCTGTATGTTCACCGTTAGCAATGCGAATGGCTGAACATTTTAGATCCTGCAAAGAGCGCAAAACGATTTGACAGTGGGAGCCAATAAg                                                                                                    >  1:248254/1‑132 (MQ=255)                        caTACGTAGCGCCAGGTCAGTAATTGGCGAACCAATCTTCCGGCCTGTATGTTCACCGTTAGCAATGCGAATGGCTGAACATTTTAGATCCTGCAAAGAGCGCAAAACGATTTCACAGTGGGAGCCAATAAg                                                                                                    <  2:248254/132‑1 (MQ=255)                             gTAGCGCCAGGTCAGTAATTGGCGAACCAATCTTCCGGCCTGTATGTTCACCGTTAGCAATGCGAATGGCTGAACATTTTAGATCCTGCAAAGAGCGCAAAACGATTTCACAGTGGGAGCCAATAAGCATCGCTAACCCg                                                                                       >  1:187877/1‑140 (MQ=255)                                   ccAGGTCAGTAAGTGGCGAACCAATCTTCCGGCCTGTATGTTCACCGCTAGCAATGCGTATGGCTGAACATTTGAGATCCTGCAAAGAGCGCTAACCGCTTTTACAGTGGGGGCCAATAAGCCTCGCTAACCCGCccacc                                                                                 >  2:219457/1‑140 (MQ=255)                                      ggTCAGTAATTGGCGAACCAATCTTCCGGCCTGTATGTTCACCGTTAGCAATGCGAATGGCTGAACATTTTAGATCCTGCAAAGAGCGCAAAACTATTTCACAGTGGGAGCCAATAAGCATCGCTAACCCGTCcaccac                                                                               <  1:111533/139‑1 (MQ=255)                                      ggTCAGTAATTGGCGAACCAATCTTCCGGCCTGTATGTTCACCGTTAGCAATGCGAATGGCTGAACATTTTAGATCCTGCAAAGAGCGCAAAACTATTTCACAGTGGGAGCCAATAAGCATCGCTAACCCGTCcaccac                                                                               >  2:111533/1‑139 (MQ=255)                                      ggTCAGTAATTGGCGAACCAATCTTCCGGCCTGTATGTTCACCGTTAGCAATGCGAATGGCTGAACATTTTAGATCCTGCAAAGAGCGCAAAACGATTTCACAGTGGGAGCCAATAAGCATCGCTAACCCGTc                                                                                     >  1:324495/1‑133 (MQ=255)                                      ggTCAGTAATTGGCGAACCAATCTTCCGGCCTGTATGTTCACCGTTAGCAATGCGAATGGCTGAACATTTTAGATCCTGCAAAGAGCGCAAAACGATTTCACAGTGGGAGCCAATAAGCATCGCTAACCCGTc                                                                                     <  2:324495/133‑1 (MQ=255)                                      ggTCAGTAATTGGCGAACCAATCTTCCGGCCTGTATGTTCACCGTTAGCAATGCGAATGGCTGAACATTTTAGATCCTGCAAAGAGCGCAAAACGATTTCACAGTGGGAGCCAATAAGCATCGCTAACCCGTCCaccacc                                                                              >  1:251259/1‑140 (MQ=255)                                                       ccAATCTTCCGGCCTGTATGTTCACCGTTAGCAATGCGAATGGCTGAACATTTTAGATCCTGCAAAGAGCGCAAAACGATTTCACAGTGGGAGCCAATAAGCATCGCTAACCCGTCCACCACCGCTTCGTAGGATTTCAg                                                             <  2:251259/140‑1 (MQ=255)                                                                           ttCACCGTTAGCAATGCGAATGGCTGAACATTTTAGATCCTGCAAAGAGCGCAAAACGATTTCACAGTGGGAGCCAATAAGCATCGCTAACCCGTCCACCACCGCTTCGTAGGATTTCAGAATATCAAAATCGGTCTGGt                                         <  2:73823/140‑1 (MQ=255)                                                                              aCCGTTAGCAATGCGAATGGCTGAACATTTTAGATCCTGCAAAGAGCGCAAAACGATTTCACAGTGGGAGCCAATAAGCATCGCTAACCCGTCCACCGCCGCTTCGTAGGATTTCAGAATATCAAAATCGGTCTGGTCGa                                      >  1:178004/1‑140 (MQ=255)                                                                               ccGTTAGCAATGCGAATGGCTGAACATTTTAGATCCTGCAAAGAGCGCAAAACGATTTCACAGTGGGAGCCAATAAGCATCGCTAACCCGTCCACCACCGCTTCGTAGGATTTCAGAATATCAAAATCGGTCTGGTCGaa                                     <  1:333487/140‑1 (MQ=255)                                                                                          gCGAATGGCTGAACATTTTAGATCCTGCAAAGAGCGCAAAACGATTTCACAGTGGGAGCCAATAAGCATCGCTAACCCGTCCACCACCGCTTCGTAGGATTTCAGAATATCAAAATCGGTCTGGTCGAAAGGACGTTGAt                          >  1:290182/1‑140 (MQ=255)                                                                                                   tGAACATTTTAGATCCTGCAAAGAGCGCAAAACGATTTCACAGTGGGAGCCAATAAGCATCGCTAACCCGTCCACCACCGCTTCGTAGGATTTCAGAATATCAAAATCGGTCTGGTCGAAAGGACGTTGATCCAGTAAAt                 >  1:60336/1‑140 (MQ=255)                                                                                                     aaCATTTTAGATCCTGCAAAGAGCGCAAAACGATTTCACAGTGGGAGCCAATAAGCATCGCTAACCCGTCCACCACCGCTTCGTAGGATTTCAGAATATCAAAATCGGTCTGGTCGAAAGGACGTTGATCCAGTAAATcc               <  2:312963/140‑1 (MQ=255)                                                                                                       cATTTTAGATCCTGCAAAGAGCGCAAAACGATTTCACAGTGGGAGCCAATAAGCATCGCTAACCCGTCCACCACCGCTTCGTAGGATTTCAGAATATCAAAATCGGTCTGGTCGAAAGGACGTTGATCCAGTAAATCCaa             >  1:333208/1‑140 (MQ=255)                                                                                                                  cTGCAAAGAGCGCAAAACGATTTCACAGTGGGAGCCAATAAGCATCGCTAACCCGTCCACCACCGCTTCGTAGGATTTCAGAATATCAAAATCGGTCTGGTCGAAAGGACGTTGATCCAGTAAATCCAACTCACTGGttt  >  1:183817/1‑140 (MQ=255)                                                                                                                            |                                                                                                                                  TCCGCTCCCGTCATATCGTGCAGCATACGTAGCGCCAGGTCAGTAATTGGCGAACCAATCTTCCGGCCTGTATGTTCACCGTTAGCAATGCGAATGGCTGAACATTTTAGATCCTGCAAAGAGTGCAAAACGATTTCACAGTGGGAGCCAATAAGCATCGCTAACCCGTCCACCACCGCTTCGTAGGATTTCAGAATATCAAAATCGGTCTGGTCGAAAGGACGTTGATCCAGTAAATCCAACTCACTGGTTT  >  NC_000913/3476163‑3476415

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑