Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,477,136	T→C	D95G (GAC→GGC)	fkpA ←	peptidyl‑prolyl cis‑trans isomerase FkpA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,477,136	0	T	C	100.0%	73.2 / NA	21	D95G (GAC→GGC)	fkpA	peptidyl‑prolyl cis‑trans isomerase FkpA
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (10/11);  total (10/11)

ACCTTTCTCTTTGGCAAATTTCTCGCGGTACTCTTTACCTTTTGCTTCGTTATCAGCCGCGTCTTTTTCCATCTTCGCCTGAGCAGAAGACTTCACGCGAGCTTCGAATGCTTGTAGAGTCTGTTCGATCTCTTGGTCGGAGAGTTTGCTCTTATCAGCAAATGCATCCTGAACACCAGCGATCAGCTGATCTTTATCCAGTTTGATGCCCAGTTTTTCTTGTTCTTTTAGAGAGTTTTCCATGTAACGACCCAGCGAGGCA  >  NC_000913/3477000‑3477261                                                                                                                                         |                                                                                                                              acCTTTCTCTTTGGCAAATTTCTCGCGGTACTCTTTACCTTTTGCTTCGTTATCAGCCGCGTCTTTTTCCATCTTCGCCTGAGCAGAAGACTTCACGCGAGCTTCGAATGCTTGTAGAGTCTGTTCGATCTCTTGGCCgg                                                                                                                            <  2:264177/140‑1 (MQ=255)      tctcTTTGGCAAATTTCTCGCGGTACTCTTTACCTTTTGCTTCGTTATCAGCCGCGTCTTTTTCCATCTTCGCCTGAGCAGAAGACTTCACGCGAGCTTCGAATGCTTGTAGAGTCTGTTCGATCTCTTGGCCGGAGAGt                                                                                                                       <  2:325422/140‑1 (MQ=255)         ctTTGGCAAATTTCTCGCGGTACTCTTTACCTTTTGCTTCGTTATCAGCCGCGTCTTTTTCCATCTTCGCCTGAGCAGAAGACTTCACGCGAGCTTCGAATGCTTGTAGAGTCTGTTCGATCTCTTGGCCGGAGAGTTTg                                                                                                                    <  2:144661/140‑1 (MQ=255)               cAAATTTCTCGCGGTACTCTTTACCTTTTGCTTCGTTATCAGCCGCGTCTTTTTCCATCTTCGCCTGAGCAGAAGACTTCACGCGAGCTTCGAATGCTTGTAGAGTCTGTTCGATCTCTTGGCCGGAGAGTTTGCTCTTa                                                                                                              <  1:162316/140‑1 (MQ=255)               cAAATTTCTCGCGGTACTCTTTACCTTTTGCTTCGTTATCAGCCGCGTCTTTTTCCATCTTCGCCTGAGCAGAAGACTTCACGCGAGCTTCGAATGCTTGTAGAGTCTGTTCGATCTCTTGGCCGGAGAGTTTGCTCTTa                                                                                                              >  2:171567/1‑140 (MQ=255)                   tttCTCGCGGTACTCTTTACCTTTTGCTTCGTTATCAGCCGCGTCTTTTTCCATCTTCGCCTGAGCAGAAGACTTCACGCGAGCTTCGAATGCTTGTAGAGTCTGTTCGATCTCTTGGCCGGAGAGTTTGCTCTTATCAg                                                                                                          <  1:120343/140‑1 (MQ=255)                        cgcgGTACTCTTTACCTTTTGCTTCGTTATCAGCCGCGTCTTTTTCCATCTTCGCCTGAGCAGAAGACTTCACGCGAGCTTCGAATGCTTGTAGAGTCTGTTCGATCTCTTGGCCGGAGAGTTTGCTCTTATCAGCAAAt                                                                                                     <  1:349310/140‑1 (MQ=255)                        cgcgGTACTCTTTACCTTTTGCTTCGTTATCAGCCGCGTCTTTTTCCATCTTCGCCTGAGCAGAAGACTTCACGCGAGCTTCGAATGCTTGTAGAGTCTGTTCGATCTCTTGGCCGGAGAGTTTGCTCTTATCAGCAAAt                                                                                                     <  2:71279/140‑1 (MQ=255)                                       cTTTTGCTTCGTTATCAGCCGCGTCTTTTTCCATCTTCGCCTGAGCAGAAGACTTCACGCGAGCTTCGAATGCTTGTAGAGTCTGTTCGATCTCTTGGCCGGAGAGTTTGCTCTTATCAGCAAATGCATCCTGAACACCa                                                                                      >  1:219697/1‑140 (MQ=255)                                                 gTTATCAGCCGCGTCTTTTTCCATCTTCGCCTGAGCAGAAGACTTCACGCGAGCTTCGAATGCTTGTAGAGTCTGTTCGATCTCTTGGCCGGAGAGTTTGCTCTTATCAGCAAATGCATCCTGAACACCAGCGATCAGCt                                                                            <  1:79888/140‑1 (MQ=255)                                                                                            ttCACGCGAGCTTCGAATGCTTGTAGAGTCTGTTCGATCTCTTGGCCGGAGAGTTTGCTCTTATCAGCAAATGCATCCTGAACACCAGCGATCAGCTGATCTTTATCCAGTTTGATGCCCAGTTTTTCTTGTTCTTTTag                                 >  1:162667/1‑140 (MQ=255)                                                                                                 gcgAGCTTCGAATGCTTGTAGAGTCTGTTCGATCTCTTGGCCGGAGAGTTTGCTCTTATCAGCAAATGCATCCTGAACACCAGCGATCAGCTGATCTTTATCCAGTTTGATGCCCAGTTTTTGTTGTTCTTTTAGAGAGt                            >  2:316428/1‑140 (MQ=255)                                                                                                 gcgAGCTTCGAATGCTTGTAGAGTCTGTTCGATCTCTTGGCCGGAGAGTTTGCTCTTATCAGCAAATGCATCCTGAACACCAGCGATCAGCTGATCTTTATCCAGTTTGATGCCCAGTTTTTCTTGTTCTTTTAGAGAGt                            >  2:178820/1‑140 (MQ=255)                                                                                                     gCTTCGAATGCTTGTAGAGTCTGTTCGATCTCTTGGCCGGAGAGTTTGCTCTTATCAGCAAATGCATCCTGAACACCAGCGATCAGCTGATCTTTATCCAGTTTGATGCCCAGTTTTTCTTGTTCTTTTAGAGAGTTTTc                        <  1:354247/140‑1 (MQ=255)                                                                                                      cTTCGAATGCTTGTAGAGTCTGTTCGATCTCTTGGCCGGAGAGTTTGCTCTTATCAGCAAATGCATCCTGAACACCAGCGATCAGCTGATCTTTATCCAGTTTGATGCCCAGTTTTTCTTGTTCTTTTAGAGAGtttt                         <  1:340088/138‑1 (MQ=255)                                                                                                      cTTCGAATGCTTGTAGAGTCTGTTCGATCTCTTGGCCGGAGAGTTTGCTCTTATCAGCAAATGCATCCTGAACACCAGCGATCAGCTGATCTTTATCCAGTTTGATGCCCAGTTTTTCTTGTTCTTTTAGAGAGtttt                         >  2:340088/1‑138 (MQ=255)                                                                                                                   tAGAGTCTGTTCGATCTCTTGGCCGGAGAGTTTGCTCTTATCAGCAAATGCATCCTGAACACCAGCGATCAGCTGATCTTTATCCAGTTTGATGCCCAGTTTTTCTTGTTCTTTTAGAGAGTTTTCCATGTAACGACCCa          >  1:132907/1‑140 (MQ=255)                                                                                                                   tAGAGTCTGTTCGATCTCTTGGCCGGAGAGTTTGCTCTTATCAGCAAATGCATCCTGAACACCAGCGATCAGCTGATCTTTATCCAGTTTGATGCCCAGTTTTTCTTGTTCTTTTAGAGAGTTTTCCATGTAACGACCCa          <  2:219635/140‑1 (MQ=255)                                                                                                                          tGTTCGATCTCTTGGCCGGAGAGTTTGCTCTTATCAGCAAATGCATCCTGAACACCAGCGATCAGCTGATCTTTATCCAGTTTGATGCCCAGTTTTTCTTGTTCTTTTAGAGAGTTTTCCATGTAACGACCCAGCGAGGc   >  1:337309/1‑140 (MQ=255)                                                                                                                           gTTCGATCTCTTGGCCGGAGAGTTTGCTCTTATCAGCAAATGCATCCTGAACACCAGCGATCAGCTGATCTTTATCCAGTTTGATGCCCAGTTTTTCTTGTTCTTTTAGAGAGTTTTCCATGTAACGACCCAGCGAGGCa  >  2:56207/1‑140 (MQ=255)                                                                                                                           gTTCGATCTCTTGGCCGGAGAGTTTGCTCTTATCAGCAAATGCATCCTGAACACCAGCGATCAGCTGATCTTTATCCAGTTTGATGCCCAGTTTTTCTTGTTCTTTTAGAGAGTTTTCCATGTAACGACCCAGCGAGGCa  >  2:94440/1‑140 (MQ=255)                                                                                                                                         |                                                                                                                              ACCTTTCTCTTTGGCAAATTTCTCGCGGTACTCTTTACCTTTTGCTTCGTTATCAGCCGCGTCTTTTTCCATCTTCGCCTGAGCAGAAGACTTCACGCGAGCTTCGAATGCTTGTAGAGTCTGTTCGATCTCTTGGTCGGAGAGTTTGCTCTTATCAGCAAATGCATCCTGAACACCAGCGATCAGCTGATCTTTATCCAGTTTGATGCCCAGTTTTTCTTGTTCTTTTAGAGAGTTTTCCATGTAACGACCCAGCGAGGCA  >  NC_000913/3477000‑3477261

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑