Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,546,895	T→C	F113L (TTC→CTC)	gntT →	high‑affinity gluconate transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,546,895	0	T	C	100.0%	45.8 / NA	14	F113L (TTC→CTC)	gntT	high‑affinity gluconate transporter
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (6/8);  total (6/8)

CTGCGGTGGCGCACAACGTATCGCCACCACGCTGATTGCCAAATTTGGTAAAAAACACATCCAGTGGGCGGTGGTACTGACCGGTTTTACCGTTGGTTTTGCCCTGTTCTATGAAGTGGGCTTTGTGCTGATGCTGCCGCTGGTGTTTACCATCGCGGCTTCTGCGAATATTCCACTGCTGTATGTTGGTGTACCAATGGCGGCTGCACTGTCTGTGACCCACGGCTTCCTGCC  >  NC_000913/3546789‑3547022                                                                                                           |                                                                                                                                cTGCGGTGGCGCACAACGTATCGCCACCACGCTGATTGCCAAATTTGGTAAAAAACACATCCAGTGGGCGGTGGTACTGACCGGTTTTACCGTTGGTTTTGCCCTGCTCTATGAAGTGGGCTTTGTGCTGATGCTGCCGc                                                                                                <  1:186130/140‑1 (MQ=255)                        ccaccaCGCTGATTGCAAAATTTGGTAAAAAACACATCCAGTGGGCGGTGGTACTGACCGGTTTTACCGTTGGTTTTGCCCTGCTCTATGAAGTGGGCTTTGTGCTGATGCTGCCGCTGGTGTTTACCATCGCGGCTTCt                                                                         <  1:345521/140‑1 (MQ=255)                              cGCTGATTGCCAAATTTGGTAAAAAACACATCCAGTGGGCGGTGGTACTGACCGGTTTTACCGTTGGTTTTGCCCTGCTCTATGAAGTGGGCTTTGTGCTGATGCTGCCGCTGGTGTTTACCATCGCGGCTTCTGCGAat                                                                   >  2:50977/1‑140 (MQ=255)                                  gATTGCCAAATTTGGTAAAAAACACATCCAGTGGGCGGTGGTACTGACCGGTTTTACCGTTGGTTTTGCCCTGCTCTATGAAGTGGGCTTTGTGCTGATGCTGCCGCT‑GTGTTTACCATCGCGGCTTCTGCGAATATTcc                                                              <  2:100592/140‑1 (MQ=255)                                                                 tGGGCGGTGGTACTGACCGGTTTTACCGTTGGTTTTGCCCTGCTCTATGAAGTGGGCTTTGTGCTGATGCTGCCGCTGGTGTTTACCATCGCGGCTTCTGCGAATATTCCACTGCTGTATGt                                                  <  1:49999/122‑1 (MQ=255)                                                                 tGGGCGGTGGTACTGACCGGTTTTACCGTTGGTTTTGCCCTGCTCTATGAAGTGGGCTTTGTGCTGATGCTGCCGCTGGTGTTTACCATCGCGGCTTCTGCGAATATTCCACTGCTGTATGt                                                  >  2:49999/1‑122 (MQ=255)                                                                 tGGGCGGTGGTACTGACCGGTTTTACCGTTGGTTTTGCCCTGCTCTATGAAGTGGGCTTTGTGCTGATGCTGCCGCTGGTGTTTACCATCGCGGCTTCTGCGAATATTCCACTGCTGTATGTTGGTGTACCAATggcggc                                >  1:3000/1‑140 (MQ=255)                                                                     cGGTGGTACTGACCGGTTTTACCGTTGGTTTTGCCCTGCTCTATGAAGTGGGCTTTGTGCTGATGCTGCCGCTGGTGTTTACCATCGCGGCTTCTGCGAATATTCCACTGCTGTATGTTGGTGTACCAATGGCGGCTGCa                            <  1:50977/140‑1 (MQ=255)                                                                          gtACTGACCGGTTTTATCGTTGGTTTTGCCCTGCTCTATGAAGTGGGCTTTGTGCTGATGCTGCCGCTGGTGTTTACCATCGCGGCTTCTGCGAATATTCCACTGCTGTATGTTGGTGTACCAATGGCGGCTGCAc                           <  1:201337/136‑1 (MQ=255)                                                                          gtACTGACCGGTTTTATCGTTGGTTTTGCCCTGCTCTATGAAGTGGGCTTTGTGCTGATGCTGCCGCTGGTGTTTACCATCGCGGCTTCTGCGAATATTCCACTGCTGTATGTTGGTGTACCAATGGCGGCTGCAc                           >  2:201337/1‑136 (MQ=255)                                                                           tACTGACCGGTTTTACCGTTGGTTTTGCCCTGCTCTATGAAGTGGGCTTTGTGCTGATGCTGCCGCTGGTGTTTACCATCGCGGCTTCTGCGAATATTCCACTGCTGTATGTTGGTGTACCAATggcgg                                 <  1:43846/129‑1 (MQ=255)                                                                           tACTGACCGGTTTTACCGTTGGTTTTGCCCTGCTCTATGAAGTGGGCTTTGTGCTGATGCTGCCGCTGGTGTTTACCATCGCGGCTTCTGCGAATATTCCACTGCTGTATGTTGGTGTACCAATggcgg                                 >  2:43846/1‑129 (MQ=255)                                                                                              tGGTTTTGCCCTGCTCTATGAAGTGGGCTTTGTGCTGATGCTGCCGCTGGTGTTTACCATCGCGGCTTCTGCGAATATTCCACTGCTGTATGTTGGTGTACCAATGGCGGCTGCACTGTCTGTGACCCACGGCTTCCTGc   <  1:130013/140‑1 (MQ=255)                                                                                               ggTTTTGCCCTGCTCTATGAAGTGGGCTTTGTGCTGATGCTGCCGCTGGTGTTTACCATCGCGGCTTCTGCGAATATTCCACTGCTGTATGTTGGTGTACCAATGGCGGCTGCACTGTCTGTGACCCACGGCTTCCTGcc  >  1:199245/1‑140 (MQ=255)                                                                                                           |                                                                                                                                CTGCGGTGGCGCACAACGTATCGCCACCACGCTGATTGCCAAATTTGGTAAAAAACACATCCAGTGGGCGGTGGTACTGACCGGTTTTACCGTTGGTTTTGCCCTGTTCTATGAAGTGGGCTTTGTGCTGATGCTGCCGCTGGTGTTTACCATCGCGGCTTCTGCGAATATTCCACTGCTGTATGTTGGTGTACCAATGGCGGCTGCACTGTCTGTGACCCACGGCTTCCTGCC  >  NC_000913/3546789‑3547022

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑