Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,727,522	C→T	G76D (GGT→GAT)	yiaB ←	conserved inner membrane protein YiaB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,727,522	0	C	T	100.0%	94.6 / NA	28	G76D (GGT→GAT)	yiaB	conserved inner membrane protein YiaB
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (13/15);  total (13/15)

TGTAAGAGCTTTATGTTGCTCATGCCGAGCGAAACAAACGCATTTGACCAAACAAGCAGACAAAAAAGGCGATGGGATAGATCACCATTTCATAAGTATTGATAGGTGCGTTTAAAACACCGATAAACAAGAGACCGATAGTGATTAACGCAACTAACTGGCAGATATGGGTAAAAAAATCATCCAGTTGCCCGAGTTTTTCTGCGCGAAGATATGCATAGTTGCCAAAAGTTGCTGTCATTAACACGCCAAGAAAATAGCCTTTTCC  >  NC_000913/3727388‑3727655                                                                                                                                       |                                                                                                                                      tGTAAGAGCTTTATGTTGCTCATGCCGAGCGAAACAAACGCATTTGACCAAACAAGCAGACAAAAAAGGCGATGGGATAGATCACCATTTCATAAGTATTGATAGGTGCGTTTAAAACACCGATAAACAAGAGATCGATa                                                                                                                                  <  1:190226/140‑1 (MQ=255)       agCTTTATGTTGCTCATGCCGAGCGAAACAAACGCATTTGACCAAACAAGCAGACAAAAAAGGCGATGGGATAGATCACCATTTCATAAGTATTGATAGGTGCGTTTAAAACACCGATAAACAAGAGATCGATAGTGAtt                                                                                                                            >  1:43721/1‑140 (MQ=255)         cTTTATGTTGCTCATGCCTAGCGAAACAAACGCATTTGACCAAACAAGCAGACAAAAAAGGCGATGGGATAGATCACCATTTCATAAGTATTGATAGGTGCGTTTAAAACACCGATAAACAAGAGATCGATAGTGATTaa                                                                                                                          >  1:43694/1‑140 (MQ=255)              tGTTGCTCATGCCGAGCGAAACAAACGCATTTGACCAAACAAGCAGACAAAAAAGGCGATGGGATAGATCACCATTTCATAAGTATTGATAGGTGCGTTTAAAACACCGATAAACAAGAGATCGATAGTGATTAACGCaa                                                                                                                     <  2:292940/140‑1 (MQ=255)                   cTCATGCCGAGCGAAACAAACGCATTTGACCAAACAAGCAGACAAAAAAGGCGATGGGATAGATCACCATTTCATAAGTATTGATAGGTGCGTTTAAAACACCGATAAACAAGAGATCGATAGTGATTAACGCaactaac                                                                                                                >  2:162091/1‑140 (MQ=255)                            aGCGAAACAAACGCATTTGACCAAACAAGCAGACAAAAAAGGCGATGGGATAGATCACCATTTCATAAGTATTGATAGGTGCGTTTAAAACACCGATAAACAAGAGATCGATAGTGATTAACGCAACTAACTGGCAGata                                                                                                       >  2:319911/1‑140 (MQ=255)                             gCGAAACAAACGCATTTGACCAAACAAGCAGACAAAAAAGGCGATGGGATAGATCACCATTTCATAAGTATTGATAGGTGCGTTTAAAACACCGATAAACAAGAGATCGATAGTGATTAACGCAACTAACTGGCAGatat                                                                                                      >  1:349811/1‑140 (MQ=255)                              cGAAACAAACGCATTTGACCAAACAAGCAGACAAAAAAGGCGATGGGATAGATCACCATTTCATAAGTATTGATAGGTGCGTTTAAAACACCGATAAACAAGAGATCGATAGTGATTAACGCAACTAACTGGCAGATATg                                                                                                     >  2:92695/1‑140 (MQ=255)                                                 cAAACAAGCAGACAAAAAAGGCGATGGGATAGATCACCATTTCATAAGTATTGATAGGTGCGTTTAAAACACCGATAAACAAGAGATCGATAGTGATTAACGCAACTAACTGGCAGATATGGGTAAAAAAATCATCCAGt                                                                                  >  2:44034/1‑140 (MQ=255)                                                                   aGGCGATGGGATAGATCACCATTTCATAAGTATTGATAGGTGCGTTTAAAACACCGATAAACAAGAGATCGATAGTGATTAACGCAACTAACTGGCAGATATGGGTAAAAAAATCATCCAGTTGCCCGAGTTTTTCTgcg                                                                <  1:92695/140‑1 (MQ=255)                                                                   aGGCGATGGGATAGATCACCATTTCATAAGTATTGATAGGTGCGTTTAAAACACCGATAAACAAGAGATCGATAGTGATTAACGCAACTAACTGGCAGATATGGGTAAAAAAATCATCCAGTTGCCCGAGTTTTTCTgcg                                                                <  2:274914/140‑1 (MQ=255)                                                                       gATGGGATAGATCACCATTTCATAAGTATTGATAGGTGCGTTTAAAACACCGATAAACAAGAGATCGATAGTGATTAACGCAACTAACTGGCAGATATGGGTAAAAAAATCATCCAGTTGCCCGAGTTTTTCTGCGCGaa                                                            <  2:43694/140‑1 (MQ=255)                                                                       gATGGGATAGATCACCATTTCATAAGTATTGATAGGTGCGTTTAAAACACCGATAAACAAGAGATCGATAGTGATTAACGCAACTAACTGGCAGATATGGGTAAAAAAATCATCCAGTTGCCCGAGTTTTTCTGCGCGaa                                                            <  2:349811/140‑1 (MQ=255)                                                                       gATGGGATAGATCACCATTTCATAAGTATTGATAGGTGCGTTTAAAACACCGATAAACAAGAGATCGATAGTGATTAACGCAACTAACTGGCAGATATGGGTAAAAAAATCATCCAGTTGCCCGAGTTTTTCTGCGCGaa                                                            <  2:188897/140‑1 (MQ=255)                                                                        aTGGGATAGATCACCATTTCATAAGTATTGATAGGTGCGTTTAAAACACCGATAAACAAGAGATCGATAGTGATTAACGCAACTAACTGGCAGATATGGGTAAAAAAATCATCCAGTTGCCCGAGTTTTTCTGCGCGAAg                                                           <  2:43721/140‑1 (MQ=255)                                                                                      cATTTCATAAGTATTGATAGGTGCGTTTAAAACACCGATAAACAAGAGATCGATAGTGATTAACGCAACTAACTGGCAGATATGGGTAAAAAAATCATCCAGTTGCCCGAGTTTTTCTGCGCGaa                                                            <  1:17000/125‑1 (MQ=255)                                                                                      cATTTCATAAGTATTGATAGGTGCGTTTAAAACACCGATAAACAAGAGATCGATAGTGATTAACGCAACTAACTGGCAGATATGGGTAAAAAAATCATCCAGTTGCCCGAGTTTTTCTGCGCGaa                                                            >  2:17000/1‑125 (MQ=255)                                                                                          tCATAAGTATTGATAGGTGCGTTTAAAACACCGAGAAATAAGAGATCGATAGTGATTAACGCAACTAACTGGCAGATATGGGTAAAAAAATCATCCAGTTGCCCGAGTTTTTCTGCGCGAAGATATGCATAGTTGCCaaa                                         >  2:314895/1‑140 (MQ=255)                                                                                               aGTATTGATAGGTGCGTTTAAAACACCGATAAACAAGAGATCGATAGTGATTAACGCAACTAACTGGCAGATATGGGTAAAAAAATCATCCAGTTGCCCGAGTTTTTCTGCGCGAAGATATGCATAGTTGCCAAAAGTTg                                    <  1:97631/140‑1 (MQ=255)                                                                                               aGTATTGATAGGTGCGTTTAAAACACCGATAAACAAGAGATCGATAGTGATTAACGCAACTAACTGGCAGATATGGGTAAAAAAATCATCCAGTTGCCCGAGTTTTTCTGCGCGAAGATATGCATAGTTGCCAAAAGTTg                                    <  1:319911/140‑1 (MQ=255)                                                                                               aGTATTGATAGGTGCGTTTAAAACACCGATAAACAAGAGATCGATAGTGATTAACGCAACTAACTGGCAGATATGGGTAAAAAAATCATCCAGTTGCCCGAGTTTTTCTGCGCGAAGATATGCATAGTTGCCAAAAGTTg                                    <  1:162091/140‑1 (MQ=255)                                                                                                   ttGATAGGTGCGTTTAAAACACCGATAAACAAGAGATCGATAGTTATTAACGCAACTAACTGGCAGATATGGGTAAAAAAATCATCCAGTTGCCCGAGTTTTTCTGCGCGAAGATATGCATAGTTGCCAAAAGTTGCTGt                                >  2:32091/1‑140 (MQ=255)                                                                                                         ggTGCGTTTAAAACACCGATAAACAAGAGATCGATAGTGATTAACGCAACTAACTGGCAGATATGGGTAAAAAAATCATCCAGTTGCCCGAGTTTTTCTGCGCGAAGATATGCATAGTTGCCAAAAGTTGCTGTCATTaa                          <  1:44034/140‑1 (MQ=255)                                                                                                             cGTTTAAAACACCGATAAACAAGAGATCGATAGTGATTAACGCAACTAACTGGCAGATATGGGTAAAAAAATCATCCAGTTGCCCGAGTTTTTCTGCGCGAAGATATGCATAGTTGCCAAAAGTTGCTGTCATTAACACg                      >  1:83401/1‑140 (MQ=255)                                                                                                                ttAAAACACCGATAAACAAGAGATCGATAGTGATTAACGCAACTAACTGGCAGATATGGGTAAAAAAATCATCCAGTTGCCCGAGTTTTTCTGCGCGAAGATATGCATAGTTGCCAAAAGTTGCTGTCATTAACACGGCa                   >  2:217381/1‑140 (MQ=255)                                                                                                                               aCAAGAGATCGATAGTTATTAACGCAACTAACTGGCAGATATGGGTAAAAAAATCATCCAGTTGCCCGAGTTTTTCTGCGCGAAGATATGCATAGTTGCCAAAAGTTGCTGTCATTAACACGCCAAGAAAATAGCCtttt    <  1:32091/140‑1 (MQ=255)                                                                                                                               aCAAGAGATCGATAGTGATTAACGCAACTAACTGGCAGATATGGGTAAAAAAATCATCCAGTTGCCCGAGTTTTTCTGCGCGAAGATATGCATAGTTGCCAAAAGTTGCTGTCATTAACACGCCAAGAAAATAGCCtttt    <  2:298670/140‑1 (MQ=255)                                                                                                                                 aaGAGATCGATAGTGATTAACGCAACTAACTGGCAGATATGGGTAAAAAAATCATCCAGTTGCCCGAGTTTTTCTGCGCGAAGATATGCATAGTTGCCAAAAGTTGCTGTCATTAACACGCCAAGAAAATAGCCTTTTcc  >  2:175130/1‑140 (MQ=255)                                                                                                                                       |                                                                                                                                      TGTAAGAGCTTTATGTTGCTCATGCCGAGCGAAACAAACGCATTTGACCAAACAAGCAGACAAAAAAGGCGATGGGATAGATCACCATTTCATAAGTATTGATAGGTGCGTTTAAAACACCGATAAACAAGAGACCGATAGTGATTAACGCAACTAACTGGCAGATATGGGTAAAAAAATCATCCAGTTGCCCGAGTTTTTCTGCGCGAAGATATGCATAGTTGCCAAAAGTTGCTGTCATTAACACGCCAAGAAAATAGCCTTTTCC  >  NC_000913/3727388‑3727655

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑