Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,845,884	C→T	A428A (GCG→GCA)	uhpT ←	hexose‑6‑phosphate:phosphate antiporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,845,884	0	C	T	100.0%	24.3 / NA	9	A428A (GCG→GCA)	uhpT	hexose‑6‑phosphate:phosphate antiporter
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (5/4);  total (5/4)

CTGTCAACTGCTGAATTTTTTTCTCGCGGCGGATTTTGCGTTCTTCCATTACCGCCACTATCGCCATCAGGCAGATACAACCAATCGCGGCGATATCCAGCGCGGCGAAGGTGCCTGCCCAGCCGGTAAGGCCGAATACCGGCGTCCCATCGGCAATCATTCCCAGACCTAACTTGGCAAAGCTGTCACCAATCAGGTAAGCAAAGGTGCCTTTAATACCATCGGC  >  NC_000913/3845784‑3846009                                                                                                     |                                                                                                                              cTGTCAACTGCTGAATTTTTTTCTCGCGGCGGATTTTGCGTTCTTCCATTACCGCCACTATCGCCATCAGGCAGATACAACCAATCGCGGCGATATCCAGTGCGGCGAAGGTGCCTGCCCAGCCGGTAAGGCCGAATAcc                                                                                        >  1:81034/1‑140 (MQ=255)  tGTCAACTGCTGAATTTTTTTCTCGCGGCGGATTTTGCGTTCTTCCATTACCGCCACTATCGCCATCAGGCAGATACAACCAATCGCGGCGATATCCAGTGCGGCGAAGGTGCCTGCCCAGCCGGTAAGGCCGAATACCg                                                                                       >  2:215516/1‑140 (MQ=255)                               gttttttGCTTTCTTCATTTACCGCCACTATCGCCATCAGGCAGATACAACCAATCGCGGCGATATCCAGTGCGGCGAAGGTGCCTGCCCAGCCGGTAAGGCCGAATACCGGCGTCCCATCGGCAATCATTCCCAGACCt                                                          <  2:81034/137‑1 (MQ=255)                                        tttctccCATTCCCGCCACTATCGCCATCAGGCAGATACAACCAATCGCGGCGATATCCAGTGCGGCGAAGGTGCCTGCCCAGCCGGTAAGGCCGAATACCGGCGTCCCATCGGCAATCATTCCCAGACCTAACTTGGCa                                                 <  1:276726/139‑1 (MQ=255)                                                           tATCGCCATCAGGCAGATACAACCAATCGCGGCGATATCCAGTGCGGCGAAGGTGCCTGCCCAGCCGGTAAGGCCGAATACCGGCGTCCCATCGGCAATCATTCCCAGACCTAACTTGGCAAAGCTGTCACCAATCAGGt                              >  1:194065/1‑140 (MQ=255)                                                                            tACAACCAATCGCGGCGATATCCAGTGCGGCGAAGGTGCCTGCCCAGCCGGTAAGGCCGAATACCGGCGTCCCATCGGCAATCATTCCCAGACCTAACTTGGCAAAGCTGTCACCAATCAGGTAAGCAAAGGTGCCTTTa             <  2:194065/140‑1 (MQ=255)                                                                                     tCGCGGCGATATCCAGTGCGGCGAAGGTGCCTGCCCAGCCGGTAAGGCCGAATACCGGCGTCCCATCGGCAATCATTCCCAGACCTAACTTGGCAAAGCTGTCACCAATCAGGTAAGCAAAGGTGCCTTTa             >  1:108539/1‑131 (MQ=255)                                                                                     tCGCGGCGATATCCAGTGCGGCGAAGGTGCCTGCCCAGCCGGTAAGGCCGAATACCGGCGTCCCATCGGCAATCATTCCCAGACCTAACTTGGCAAAGCTGTCACCAATCAGGTAAGCAAAGGTGCCTTTa             <  2:108539/131‑1 (MQ=255)                                                                                       gcggcgATATCCAGTGCGGCGAAGGTGCCTGCCCAGCCGGTAAGGCCGAATACCGGCGTCCCATCGGCAATCATTCCCAGACCTAACTTGGCAAAGCTGTCACCAATCAGGTAAGCAAAGGTGCCTTTAATACCATCGGc  >  2:293749/1‑140 (MQ=255)                                                                                                     |                                                                                                                              CTGTCAACTGCTGAATTTTTTTCTCGCGGCGGATTTTGCGTTCTTCCATTACCGCCACTATCGCCATCAGGCAGATACAACCAATCGCGGCGATATCCAGCGCGGCGAAGGTGCCTGCCCAGCCGGTAAGGCCGAATACCGGCGTCCCATCGGCAATCATTCCCAGACCTAACTTGGCAAAGCTGTCACCAATCAGGTAAGCAAAGGTGCCTTTAATACCATCGGC  >  NC_000913/3845784‑3846009

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑