Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,999,267	A→G	T429A (ACA→GCA)	uvrD →	ssDNA translocase and dsDNA helicase ‑ DNA helicase II

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,999,267	0	A	G	100.0%	42.7 / NA	13	T429A (ACA→GCA)	uvrD	ssDNA translocase and dsDNA helicase ‑ DNA helicase II
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (9/4);  total (9/4)

GCCTGATTGCCAACCGCAACGACGACGCGGCCTTTGAGCGTGTGGTGAATACGCCAACGCGGGGTATTGGTGACCGGACGCTGGACGTGGTACGTCAGACATCGCGCGATCGCCAGTTAACACTCTGGCAGGCATGTCGTGAGCTGTTGCAGGAAAAAGCCCTCGCCGGGCGAGCTGCCAGCGCCTTGCAGCGATTTATGGAATTAATCGACGCCTTAGCGCAGG  >  NC_000913/3999169‑3999393                                                                                                   |                                                                                                                               gcCTGATTGCCAACCGCAACGACGACGCGGCCTTTGAGCGTGTGGTGAATACGCCAACGCGGGGTATTGGTGACCGGACGCTGGACGTGGTACGTCAGGCATCGCGCGATCGCCAGTTAACACTCTggcaggc                                                                                              <  1:320681/133‑1 (MQ=255) gcCTGATTGCCAACCGCAACGACGACGCGGCCTTTGAGCGTGTGGTGAATACGCCAACGCGGGGTATTGGTGACCGGACGCTGGACGTGGTACGTCAGGCATCGCGCGATCGCCAGTTAACACTCTggcaggc                                                                                              >  2:320681/1‑133 (MQ=255)  ccTGATTGCCAACCGCAACGACGACGCGGCCTTTGAGCGTGTGGTGAATACGCCAACGCGGGGTATTGGTGACCGGACGCTGGACGTGGTACGTCAGGCATCGCGCGATCGCCAGTTAACACTCTGGCAGGCATGTCGTg                                                                                      >  1:326003/1‑140 (MQ=255)             aCCGCAACGACGACGCGGCCTTTGAGCGTGTGGTGAATACGCCAACGCGGGGTATTGGTGACCGGACGCTGGACGTGGTACGTCAGGCATCGCGCGATCGCCAGTTAACACTCTGGCAGGCATGTCGTGAGCTGTTGCAg                                                                           >  2:337052/1‑140 (MQ=255)                    cgacgacgCGGCCTTTGAGCGTGTGGTGAATACGCCAACGCGGGGTATTGGTGACCGGACGCTGGACGTGGTACGTCAGGCATCGCGCGATCGCCAGTTAACACTCTGGCAGGCATGTCGTGAGCTGTTGCAGGAAAAAg                                                                    <  2:326003/140‑1 (MQ=255)                          cgcgGCCTTTGAGCGTGTGGTGAATACGCCAACGCGGGGTATTGGTGACCGGACGCTGGACGTGGTACGTCAGGCATCGCGCGATCGCCAGTTAACACTCTGGCAGGCATGTCGTGAGCTGTTGCAGGAAAAAGCCCTCg                                                              <  1:337052/140‑1 (MQ=255)                                         tgtgGTGAATACGCCAACGCGGGGTATTGGTGACCGGACGCTGGACGTGGTACGTCAGGCATCGCGCGATCGCCAGTTAACACTCTGGCAGGCATGTCGTGAGCTGTTGCAGGAAAAAGCCCTCGCCGGGCGAGCTGCCa                                               >  1:181185/1‑140 (MQ=255)                                                    cGCCAACGCGGGGTATTGGTGACCGGACGCTGGACGTGGTACGTCAGGCATCGCGCGATCGCCAGTTAACACTCTGGCAGGCATGTCGTGAGCTGTTGCAGGAAAAAGCCCTCGCCGGGCGAGCTGCCAGCGCCTTGCAg                                    >  2:13089/1‑140 (MQ=255)                                                          cgcgGGGTATTGGTGACCGGACGCTGGACGTGGTACGTCAGGCATCGCGCGATCGCCAGTTAACACTCTGGCAGGCATGTCGTGAGCTGTTGCAGGAAAAAGCCCTCGCCGGGCGAGCTGCCAGCGCCTTGCAGCGAttt                              >  2:230996/1‑140 (MQ=255)                                                                         aCCGGACGCTGGACGTGGTACGTCAGGCATCGCGCGATCGCCAGTTAACACTCTGGCAGGCATGTCGTGAGCTGTTGCAGGAAAAAGCCCTCGCCGGGCGAGCTGCCAGCGCCTTGCAGCGATTTATGGAATTAATCGAc               >  1:220064/1‑140 (MQ=255)                                                                           cGGACGCTGGACGTGGTACGTCAGGCATCGCGCGATCGCCAGTTAACACTCTGGCAGGCATGTCGTGAGCTGTTGCAGGAAAAAGCCCTCGCCGGGCGAGCTGCCAGCGCCTTGCAGCGATTTATGGAATTAATCGACGc             >  1:36983/1‑140 (MQ=255)                                                                              aCGCTGGACGTGGTACGTCAGGCATCGCGCGATCGCCAGTTAACACTCTGGCAGGCATGTCGTGAGCTGTTGCAGGAAAAAGCCCTCGCCGGGCGAGCTGCCAGCGCCTTGCAGCGATTTATGGAATTAATCGACGCCtt          >  1:49901/1‑140 (MQ=255)                                                                                      cGTGGTACGTCAGGCATCGCGCGATCGCCAGTTAACACTCTGGCAGGCATGTCGTGAGCTGTTGCAGGAAAAAGCCCTCGCCGGGCGAGCTGCCAGCGCCTTGCAGCGATTTATGGAATTAATCGACGCCTTAGCGCAgg  <  1:13089/140‑1 (MQ=255)                                                                                                   |                                                                                                                               GCCTGATTGCCAACCGCAACGACGACGCGGCCTTTGAGCGTGTGGTGAATACGCCAACGCGGGGTATTGGTGACCGGACGCTGGACGTGGTACGTCAGACATCGCGCGATCGCCAGTTAACACTCTGGCAGGCATGTCGTGAGCTGTTGCAGGAAAAAGCCCTCGCCGGGCGAGCTGCCAGCGCCTTGCAGCGATTTATGGAATTAATCGACGCCTTAGCGCAGG  >  NC_000913/3999169‑3999393

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑