Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,276,536	C→T	R356C (CGC→TGC)	pdeC →	c‑di‑GMP phosphodiesterase PdeC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,276,536	0	C	T	100.0%	33.5 / NA	11	R356C (CGC→TGC)	pdeC	c‑di‑GMP phosphodiesterase PdeC
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (8/3);  total (8/3)

CAGGGGCAAATAATGAATCCGGCAGAGTTTATTCCGCTGGCAGAAAAGGAGGGGATGATAGAACAGATAACTGATTATGTTATTGATAATGTCTTCCGCGATCTGGGCGATTACCTGGCAACACATGCAGATCGCTATGTTTCTATTAACCTGTCGGCCTCCGATTTTCATACGTCACGGTTGATAGCGCGAATCAATCAGAAAACAGAGCAATACGCGGTGCGTCCGCAGCAAATTAAATTTGAAGTGACTGAGCATGCATTTCT  >  NC_000913/4276404‑4276669                                                                                                                                     |                                                                                                                                      cAGGGGCAAATAATGAATCCGGCAGAGTTTATTCCGCTGGCAGAAAAGGAGGGGATGATAGAACAGATAACTGATTATGTTATTGATAATGTCTTCCGCGATCTGGGCGATTACCTGGCAACACATGCAGATTGCTATGt                                                                                                                                >  2:302626/1‑140 (MQ=255)                    cGGCAGAGTTTATTCCGCTGGCAGAAAAGGAGGGGATGATAGAACAGATAACTGATTATGTTATTGATAATGTCTTCCGCGATCTGGGCGATTACCTGGCAACACATGCAGATTGCTATGTTTCTATTAACCTGTCGGcc                                                                                                             >  2:162970/1‑140 (MQ=255)                                                    gggATGATAGAACAGATAACTGATTATGTTATTGATAATGTCTTCCGCGATCTGGGCGATTACCTGGCAACACATGCAGATTGCTATGTTTCTATTAACCTGTCGGCCTCCGATTTTCATACGTCACGGTTGATAGcgcg                                                                             >  2:57986/1‑140 (MQ=255)                                                                                          tGTCTTCCGCGATCTGGGCGATTACCTGGCAACACATGCAGATTGCTATGTTTCTATTAACCTGTCGGCCTCCGATTTTCATACGTCACGGTTGATAGCGCGAATCAATCAGAAAACAGAGCAATACGCGGTGCGTCCgc                                       >  2:101517/1‑140 (MQ=255)                                                                                          tGTCTTCCGCGATCTGGGCGATTACCTGGCAACACATGCAGATTGCTATGTTTCTATTAACCTGTCGGCCTCCGATTTTCATACGTCACGGTTGATAGCGCGAATCAATCAGAAAACAGAGCAATACGCGGTGCGTCCgc                                       >  2:83485/1‑140 (MQ=255)                                                                                                        tGGGCGATTACCTGGCAACACATGCAGATTGCTATGTTTCTATTAACCTGTCGGCCTCCGATTTTCATACGTCACGGTTGATAGCGCGAATCAATCAGAAAACAGAGCAATACGCGGTGCGTCCGCAGCAAATTAAAttt                         >  2:3426/1‑140 (MQ=255)                                                                                                              aTTACCTGGCAACACATGCAGATTGCTATGTTTCTATTAACCTGTCGGCCTCCGATTTTCATACGTCACGGTTGATAGCGCGAATCAATCAGAAAACAGAGCAATACGCGGTGCGTCCGCAGCAAATTAAATTTGAAGTg                   <  1:83485/140‑1 (MQ=255)                                                                                                                tACCTGGCAACACATGCAGATTGCTATGTTTCTATTAACCTGTCGGCCTCCGATTTTCATACGTCACGGTTGATAGCGCGAATCAATCAGAAAACAGAGCAATACGCGGTGCGTCCGCAGCAAATTAAATTTGAAGTGAc                 <  1:302626/140‑1 (MQ=255)                                                                                                                      gCAACACATGCAGATTGCTATGTTTCTATTAACCTGTCGGCCTCCGATTTTCATACGTCACGGTTGATAGCGCGAATCAATCAGAAAACAGAGCAATACGCGGTGCGTCCGCAGCAAATTAAATTTGAAGTGACTGAgca           <  1:57986/140‑1 (MQ=255)                                                                                                                            caTGCAGATTGCTATGTTTCTATTAACCTGTCGGCCTCCGATTTTCATACGTCACGGTTGATAGCGCGAATCAATCAGAAAACAGAGCAATACGCGGTGCGTCCGCAGCAAATTAAATTTGAAGTGACTGAGCATGCAtt     >  2:226623/1‑140 (MQ=255)                                                                                                                               gCAGATTGCTATGTTTCTACTAACCTGTCGGCCTCCGATTTTCATACGTCACGGTTGATAGCGCGAATCAATCAGAAAACAGAGCAATACGCGGTGCGTCCGCAGCAAATTAAATTTGAAGTGACTGAGCATGCATTTCt  >  2:217934/1‑140 (MQ=255)                                                                                                                                     |                                                                                                                                      CAGGGGCAAATAATGAATCCGGCAGAGTTTATTCCGCTGGCAGAAAAGGAGGGGATGATAGAACAGATAACTGATTATGTTATTGATAATGTCTTCCGCGATCTGGGCGATTACCTGGCAACACATGCAGATCGCTATGTTTCTATTAACCTGTCGGCCTCCGATTTTCATACGTCACGGTTGATAGCGCGAATCAATCAGAAAACAGAGCAATACGCGGTGCGTCCGCAGCAAATTAAATTTGAAGTGACTGAGCATGCATTTCT  >  NC_000913/4276404‑4276669

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑