Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	505,624	A→G	A237A (GCA→GCG)	ushA →	5'‑nucleotidase/UDP‑sugar hydrolase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	505,624	0	A	G	100.0%	91.3 / NA	26	A237A (GCA→GCG)	ushA	5'‑nucleotidase/UDP‑sugar hydrolase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (14/12);  total (14/12)

CCGATGAAGCGAAGCTGGTGATTCAGGAGCTGCAACAGACAGAAAAGCCAGACATTATTATCGCGGCGACCCATATGGGGCATTACGATAATGGTGAGCACGGCTCTAACGCACCGGGCGATGTGGAGATGGCACGCGCGCTGCCTGCCGGATCGCTGGCGATGATCGTCGGTGGTCACTCGCAAGATCCGGTCTGCATGGCGGCAGAAAACAAAAAACAGGTCGATTACGTGCCGGGTACGCCATGCAAACCA  >  NC_000913/505491‑505744                                                                                                                                      |                                                                                                                         ccgATGAAGCGAAGCTGGTGATTCAGGAGCTGCAACAGACAGAAAAGCCAGACATTATTATCGCGGCGACCCATATGGGGCATTACGATAATGGTGAGCACGGCTCTAACGCACCGGGCGATGTGGAGATGGcgcgcgcg                                                                                                                    <  1:15643/140‑1 (MQ=255) ccgATGAAGCGAAGCTGGTGATTCAGGAGCTGCAACAGACAGAAAAGCCAGACATTATTATCGCGGCGACCCATATGGGGCATTACGATAATGGTGAGCACGGCTCTAACGCACCGGGCGATGTGGAGATGGcgcgcgcg                                                                                                                    <  1:342516/140‑1 (MQ=255)        agcgaagcTGGTGATTCAGGAGCTGCAACAGACAGAAAAGCCAGACATTATTATCGCGGCGACCCATATGGGGCATTACGATAATGGTGAGCACGGCTCTAACGCACCGGGCGATGTGGAGATGGCGCGCGCGctgcctg                                                                                                             >  2:287590/1‑140 (MQ=255)                               tGCAACAGACAGAAAAGCCAGACATTATTATCGCGGCGACCCATATGGGGCATTACGATAATGGTGAGCACGGCTCTAACGCACCGGGCGATGTGGAGATGGCGCGCGCGCTGCCTGCCGGATCGCTGGCGATGAtcgtc                                                                                      >  1:115042/1‑140 (MQ=255)                               tGCAACAGACAGAAAAGCCAGACATTATTATCGCGGCGACCCATATGGGGCATTACGATAATGGTGAGCACGGCTCTAACGCACCGGGCGATGTGGAGATGGCGCGCGCGCTGCCTGCCGGATCGCTGGCGATGAtcgtc                                                                                      >  1:263114/1‑140 (MQ=255)                                     agacagaAAAGCCAGACATTATTATCGCGGCGACCCATATGGGGCATTACGATAATGGTGAGCACGGCTCTAACGCACCGGGCGATGTGGAGATGGCGCGCGCGCTGCCTGCCGGATCGCTGGCGATGATCGTCggtggt                                                                                <  2:43251/140‑1 (MQ=255)                                     agacagaAAAGCCAGACATTATTATCGCGGCGACCCATATGGGGCATTACGATAATGGTGAGCACGGCTCTAACGCACCGGGCGATGTGGAGATGGCGCGCGCGCTGCCTGCCGGATCGCTGGCGATGATCGTCggtggt                                                                                <  1:287590/140‑1 (MQ=255)                                           aaaaGCCAGACATTATTATCGCGGCGACCCATATGGGGCATTACGATAATGGTGAGCACGGCTCTAACGCACCGGGCGATGTGGAGATGGCGCGCGCGCTGCCTGCCGGATCGCTGGCGATGATCGTCGGTGGTCACTCg                                                                          >  2:211893/1‑140 (MQ=255)                                           aaaaGCCAGACATTATTATCGCGACGACCCATATGGGGCATTACGATAATGGTGAGCACGGCTCTAACGCACCGGGCGATGTGGAGATGGCGCGCGCGCTGCCTGCCGGATCGCTGGCGATGATCGTCGGTGGTCACTCg                                                                          >  1:212834/1‑140 (MQ=255)                                                     cATTATTATCGCGGCGACCCATATGGGGCATTACGATAATGGTGAGCACGGCTCTAACGCACCGGGCGATGTGGAGATGGCGCGCGCGCTGCCTGCCGGATCGCTGGCGATGATCGTCGGTGGTCACTCGCAAGATCCgg                                                                >  1:7223/1‑140 (MQ=255)                                                      attattATCGCGGCGACCCATATGGGGCATTACGATAATGGTGAGCACGGCTCTAACGCACCGGGCGATGTGGAGATGGCGCGCGCGCTGCCTGCCGGATCGCTGGCGATGATCGTCGGTGGTCACTCGCAAGATCCGGt                                                               <  2:263114/140‑1 (MQ=255)                                                                               ggCATTACGATAATGGTGAGCACGGCTCTAACGCACCGGGCGATGTGGAGATGGCGCGCGCGCTGCCTGCCGGATCGCTGGCGATGATCGTCGGTGGTCACTCGCAAGATCCGGTCTGCATGGCGGCAGAAAACaaaaaa                                      >  2:22717/1‑140 (MQ=255)                                                                               ggCATTACGATAATGGTGAGCACGGCTCTAACGCACCGGGCGATGTGGAGATGGCGCGCGCGCTGCCTGCCGGATCGCTGGCGATGATCGTCGGTGGTCACTCGCAAGATCCGGTCTGCATGGCGGCAGAAAACaaaaaa                                      <  1:331549/140‑1 (MQ=255)                                                                                gCATTACGATAATGGTGAGCACGGCTCTAACGCACCGGGCGATGTGGAGATGGCGCGCGCGCTGCCTACCGGATCGCTGGCGATGATCGTCGGTGGTCACTCGCAAGATCCGGTCTGCATGGCGGCAGAAAACAAAAAAc                                     >  1:205621/1‑140 (MQ=255)                                                                                   ttACGATAATGGTGAGCACGGCTCTAACGCACCGGGCGATGTGGAGATGGCGCGCGCGCTGCCTGCCGGATCGCTGGCGATGATCGTCGGTGGTCACTCGCAAGATCCGGTCTGCATGGCGGCAGAAAACAAAAAACa                                    >  2:169014/1‑138 (MQ=255)                                                                                   ttACGATAATGGTGAGCACGGCTCTAACGCACCGGGCGATGTGGAGATGGCGCGCGCGCTGCCTGCCGGATCGCTGGCGATGATCGTCGGTGGTCACTCGCAAGATCCGGTCTGCATGGCGGCAGAAAACAAAAAACa                                    <  1:169014/138‑1 (MQ=255)                                                                                    tACGATAATGGTGAGCACGGCTCTAACGCACCGGGCGATGTGGAGATGGCGCGCGCGCTGCCTGCCGGATCGCTGGCGATGATCGTCGGTGGTCACTCGCAAGATCCGGTCTGCATGGCGGCAGAAAACAAAAAACAggg                                 >  1:184065/1‑139 (MQ=255)                                                                                    tACGATAATGGTGAGCACGGCTCTAACGCACCGGGCGATGTGGAGATGGCGCGCGCGCTGCCTGCCGGATCGCTGGCGATGATCGTCGGTGGTCACTCGCAAGATCCGGTCTGCATGGCGGCAGAAAACAAAAAACAGGt                                 >  1:294423/1‑140 (MQ=255)                                                                                    tACGATAATGGTGAGCACGGCTCTAACGCACCGGGCGATGTGGAGATGGCGCGCGCGCTGCCTGCCGGATCGCTGGCGATGATCGTCGGTGGTCACTCGCAAGATCCGGTCTGCATGGCGGCAGAAAACAAAAAACAGGt                                 >  1:288409/1‑140 (MQ=255)                                                                                     aCGATAATGGTGAGCACGGCTCTAACGCACCGGGCGATGTGGAGATGGCGCGCGCGCTGCCTGCCGGATCGCTGGCGATGATCGTCGGTGGTCACTCGCAAGATCCGGTCTGCATGGCGGCAGAAAACaaaaaa                                      <  2:184733/134‑1 (MQ=255)                                                                                     aCGATAATGGTGAGCACGGCTCTAACGCACCGGGCGATGTGGAGATGGCGCGCGCGCTGCCTGCCGGATCGCTGGCGATGATCGTCGGTGGTCACTCGCAAGATCCGGTCTGCATGGCGGCAGAAAACaaaaaa                                      >  1:184733/1‑134 (MQ=255)                                                                                              gTGAGCACGGCTCTAACGCACCGGGCGATGTGGAGATGGCGCGCGCGCTGCCTGCCGGATCGCTGGCGATGATCGTCGGTGGTCACTCGCAAGATCCGGTCTGCATGGCGGCAGAAAACAAAAAACAGGTCGATTACGTg                       <  2:294423/140‑1 (MQ=255)                                                                                              gTGAGCACGGCTCTAACGCACCGGGCGATGTGGAGATGGCGCGCGCGCTGCCTGCCGGATCGCTGGCGATGATCGTCGGTGGTCACTCGCAAGATCCGGTCTGCATGGCGGCAGAAAACAAAAAACAGGTCGATTACGTg                       <  2:7223/140‑1 (MQ=255)                                                                                                       gCTCTAACGCACCGGGCGATGTGGAGATGGCGCGCGCGCTGCCTGCCGGATCGCTGGCGATGATCGTCGGTGGTCACTCGCAAGATCCGGTCTGCATGGCGGCAGAAAACAAAAAACAGGTCGATTACGTGCCGGGTACg              <  2:212834/140‑1 (MQ=255)                                                                                                       gCTCTAACGCACCGGGCGATGTGGAGATGGCGCGCGCGCTGCCTGCCGGATCGCTGGCGATGATCGTCGGTGGTCACTCGCAAGATCCGGTCTGCATGGCGGCAGAAAACAAAAAACAGGTCGATTACGTGCCGGGTACg              <  2:205621/140‑1 (MQ=255)                                                                                                       gCTCTAACGCACCGGGCGATGTGGAGATGGCGCGCGCGCTGCCTGCCGGATCGCTGGCGATGATCGTCGGTGGTCACTCGCAAGATCCGGTCTGCATGGCGGCAGAAAACAAAAAACAGGTCGATTACGTGCCGGGTACg              <  2:184065/140‑1 (MQ=255)                                                                                                                 aCCGGGCGATGTGGAGATGGCGCGCGCGCTGCCTGCCGGATCGCTGGCGATGATCGTCGGTGGTCACTCGCAAGATCCGGTCTGCATGGCGGCAGAAAACAAAAAACAGGTCGATTACGTGCCGGGTACGCCATGCAAAc    <  1:22717/140‑1 (MQ=255)                                                                                                                   cGGGCGATGTGGAGATGGCGCGCGCGCTGCCTGCCGGATCGCTGGCGATGATCGTCGGTGGTCACTCGCAAGATCCGGTCTGCATGGCGGCAGAAAACAAAAAACAGGTCGATTACGTGCCGGGTACGCCATGCAAACCa  >  1:153927/1‑140 (MQ=255)                                                                                                                                      |                                                                                                                         CCGATGAAGCGAAGCTGGTGATTCAGGAGCTGCAACAGACAGAAAAGCCAGACATTATTATCGCGGCGACCCATATGGGGCATTACGATAATGGTGAGCACGGCTCTAACGCACCGGGCGATGTGGAGATGGCACGCGCGCTGCCTGCCGGATCGCTGGCGATGATCGTCGGTGGTCACTCGCAAGATCCGGTCTGCATGGCGGCAGAAAACAAAAAACAGGTCGATTACGTGCCGGGTACGCCATGCAAACCA  >  NC_000913/505491‑505744

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑