Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,350,361	C→T	T434T (ACG→ACA)	dcuS ←	sensory histidine kinase DcuS

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,350,361	0	C	T	100.0%	70.9 / NA	22	T434T (ACG→ACA)	dcuS	sensory histidine kinase DcuS
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (11/11);  total (11/11)

ATCCCCGGTCCATCATCATTAACTTCACAGTGCAGCCAGCCGTGACGGTAGTGCAATGTTACGCTAATTTCGCCTCCGGGTTCCGGCCCTAATGCCTCCAGCGCGTTTTCTATCAGATTTCCCAACGTGGTAATCAGCGTCGCGACCTGGTCCTCACTGCCGCTGTCTGGCAGCTGGCTTTCACTGTTTAAAATCAGCGTATGGCCTAAATCGGTCGCGCGGTTAATCTTGCTGATTAAAAAACCAGC  >  NC_000913/4350224‑4350471                                                                                                                                          |                                                                                                               aTCCCCGGTCCATCATCATTAACTTCACAGTGCAGCCAGCCGTGACGGTAGTGCAATGTTACGCTAATTTCGCCTCCGGGTTCCGGCCCTAATGCCTCCAGCGCGTTTTCTATCAGATTTCCCAACGTGGTAATCAgtgt                                                                                                              >  2:137926/1‑140 (MQ=255)        gTCCATCATCATTAACTTCACAGTGCAGCCAGCCGTGACGGTAGTGCAATGTTACGCTAATTTCGCCTCCGGGTTCCGGCCCTAATGCCTCCAGCGCGTTTTCTATCAGATTTCCCAACGTGGTAATCAGTGTCGCGAcc                                                                                                       <  1:137926/140‑1 (MQ=255)               atcatTAACTTCACAGTGCAGCCAGCCGTGACGGTAGTGCAATGTTACGCTAATTTCGCCTCCGGGTTCCGGCCCTAATGCCTCCAGCGCGTTTTCTATCAGATTTCCCAACGTGGTAATCAGTGTCGCGACCTGGTCCt                                                                                                <  2:136794/140‑1 (MQ=255)                   ttAACTTCACAGTGCAGCCAGCCGTGACGGTAGTGCAATGTTACGCTAATTTCGCCTCCGGGTTCCGGCCCTAATGCCTCCAGCGCGTTTTCTATCAGATTTCCCAACGTGGTAATCAGTGTCGCGACCTGGTc                                                                                                  <  1:259409/134‑1 (MQ=255)                   ttAACTTCACAGTGCAGCCAGCCGTGACGGTAGTGCAATGTTACGCTAATTTCGCCTCCGGGTTCCGGCCCTAATGCCTCCAGCGCGTTTTCTATCAGATTTCCCAACGTGGTAATCAGTGTCGCGACCTGGTc                                                                                                  >  2:259409/1‑134 (MQ=255)                   ttAACTTCACAGTGCAGCCAGCCGTGACGGTAGTGCAATGTTACGCTAATTTCGCCTCCGGGTTCCGGCCCTAATGCCTCCAGCGCGTTTTCTATCAGATTTCCCAACGTGGTAATCAGTGTCGCGACCTGGTCCTCACt                                                                                            >  1:265850/1‑140 (MQ=255)                           acaGTGCAGCCAGCCGTGACGGTAGTGCAATGTTACGCTAATTTCGCCTCCGGGTTCCGGCCCTAATGCCTCCAGCGCGTTTTCTATCAGATTTCCCAACGTGGTAATCAGTGTCGCGACCTGGTCCTCACTGCCGCTGt                                                                                    >  1:246203/1‑140 (MQ=255)                           acaGTGCAGCCAGCCGTGACGGTAGTGCAATGTTACGCTAATTTCGCCTCCGGGTTCCGGCCCTAATGCCTCCAGCGCGTTTTCTATCAGATTTCCCAACGTGGTAATCAGTGTCGCGACCTGGTCCTCACTGCCGCTGt                                                                                    >  2:159211/1‑140 (MQ=255)                                  agccagccGTGACGGTAGTGCAATGTTACGCTAATTTCGCCTCCGGGTTCCGGCCCTAATGCCTCCAGCGCGTTTTCTATCAGATTTCCCAACGTGGTAATCAGTGTCGCGACCTGGTCCTCACTGCCGCTGTCTGGCAg                                                                             <  2:109249/140‑1 (MQ=255)                                  agccagccGTGACGGTAGTGCAATGTTACGCTAATTTCGCCTCCGGGTTCCGGCCCTAATGCCTCCAGCGCGTTTTCTATCAGATTTCCCAACGTGGTAATCAGTGTCGCGACCTGGTCCTCACTGCCGCTGTCTGGCAg                                                                             >  1:109249/1‑140 (MQ=255)                                        ccGTGACGGTAGTGCAATGTTACGCTAATTTCGCCTCCGGGTTCCGGCCCTAATGCCTCCAGCGCGTTTTCTATCAGATTTCCCAACGTGGTAATCAGTGTCGCGACCTGGTCCTCACTGCCGCTGTCTGGCAGCTGGCt                                                                       <  2:233561/140‑1 (MQ=255)                                                           ttACGCTAATTTCGCCTCCGGGTTCCGGCCCTAATGCCTCCAGCGCGTTTTCTATCAGATTTCCCAACGTGGTAATCAGTGTCGCGACCTGGTCCTCACTGCCGCTGTCTGGCAGCTGGCTTTCACTGTTTAAAATCAGc                                                    >  1:149477/1‑140 (MQ=255)                                                              cGCTAATTTCGCCTCCGGGTTCCGGCCCTAATGCCTCCAGCGCGTTTTCTATCAGATTTCCCAACGTGGTAATCAGTGTCGCGACCTGGTCCTCACTGCCGCTGTCTGGCAGCTGGCTTTCACTGTTTAAAATCAGCGTa                                                 <  2:265850/140‑1 (MQ=255)                                                                 tAATTTCGCCTCCGGGTTCCGGCCCTAATGCCTCCAGCGCGTTTTCTATCAGATTTCCCAACGTGGTAATCAGTGTCGCGACCTGGTCCTCACTGCCGCTGTCTGGCAGCTGGCTTTCACTGTTTAAAATCAGCGTATgg                                              <  1:151922/140‑1 (MQ=255)                                                                 tAATTTCGCCTCCGGGTTCCGGCCCTAATGCCTCCAGCGCGTTTTCTATCAGATTTCCCAACGTGGTAATCAGTGTCGCGACCTGGTCCTCACTGCCGCTGTCTGGCAGCTGGCTTTCACTGTTTAAAATCAGCGTATgg                                              >  2:25753/1‑140 (MQ=255)                                                                   aTTTCGCCTCCGGGTTCCGGCCCTAATGCCTCCAGCGCGTTTTCTATCAGATTTCCCAACGTGGTAATCAGTGTCGCGACCTGGTCCTCACTGCCGCTGCCTGGCAGCTGGCTTTCACTGTTTAAAATCAGCGt                                                  <  2:66945/134‑1 (MQ=255)                                                                   aTTTCGCCTCCGGGTTCCGGCCCTAATGCCTCCAGCGCGTTTTCTATCAGATTTCCCAACGTGGTAATCAGTGTCGCGACCTGGTCCTCACTGCCGCTGCCTGGCAGCTGGCTTTCACTGTTTAAAATCAGCGt                                                  >  1:66945/1‑134 (MQ=255)                                                                              gggTTCCGGCCCTAATGCCTCCAGCGCGTTTTCTATCAGATTTCCCAACGTGGTAATCAGTGTCGCGACCTGGTCCTCACTGCCGCTGTCTGGCAGCTGGCTTTCACTGTTTAAAATCAGCGTATGGCCTAAATCGGTcg                                 <  2:149477/140‑1 (MQ=255)                                                                                           aaTGCCTCCAGCGCGTTTTCTATCAGATTTCCCAACGTGGTAATCAGTGTCGCGACCTGGTCCTCACTGCCGCTGTCTGGCAGCTGGCTTTCACTGTTTAAAATCAGCGTATGGCCTAAATCGGTCGCGCGGTTAATCtt                    >  2:72316/1‑140 (MQ=255)                                                                                              gCCTCCAGCGCGTTTTCTATCAGATTTCCCAACGTGGTAATCAGTGTCGCGACCTGGTCCTCACTGCCGCTGTCTGGCAGCTGGCTTTCACTGTTTAAAATCAGCGTATGGCCTAAATCGGTCGCGCGGTTAATCTTGCt                 <  1:159211/140‑1 (MQ=255)                                                                                                  ccAGCGCGTTTTCTATCAGATTTCCCAACGTGGTAATCAGTGTCGCGACCTGGTCCTCACTGCCGCTGTCTGGCAGCTGGCTTTCACTGTTTAAAATCAGCGTATGGCCTAAATCGGTCGCGCGGTTAATCTTGCTGAtt             >  2:48009/1‑140 (MQ=255)                                                                                                      cgcgTTTTCTATCAGATTTCCCAACGTGGTAATCAGTGTCGCGACCTGGTCCTCACTGCCGCTGTCTGGCAGCTGGCTTTCACTGTTTAAAATCAGCGTATGGCCTAAATCGGTCGCGCGGTTAATCTTGCTGATTaaaa         >  2:239737/1‑140 (MQ=255)                                                                                                             tCTATCAGATTTCCCAACGTGGTAATCAGTGTCGCGACCTGGTCCTCACTGCCGCTGTCTGGCAGCTGGCTTTCACTGTTTAAAATCAGCGTATGGCCTAAATCGGTCGCGCGGTTAATCTTGCTGATTAAAAAACCAGc  <  2:246203/140‑1 (MQ=255)                                                                                                                                          |                                                                                                               ATCCCCGGTCCATCATCATTAACTTCACAGTGCAGCCAGCCGTGACGGTAGTGCAATGTTACGCTAATTTCGCCTCCGGGTTCCGGCCCTAATGCCTCCAGCGCGTTTTCTATCAGATTTCCCAACGTGGTAATCAGCGTCGCGACCTGGTCCTCACTGCCGCTGTCTGGCAGCTGGCTTTCACTGTTTAAAATCAGCGTATGGCCTAAATCGGTCGCGCGGTTAATCTTGCTGATTAAAAAACCAGC  >  NC_000913/4350224‑4350471

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑