Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,390,086	A→G	V92A (GTT→GCT)	psd ←	phosphatidylserine decarboxylase proenzyme

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,390,086	0	A	G	100.0%	99.3 / NA	28	V92A (GTT→GCT)	psd	phosphatidylserine decarboxylase proenzyme
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (15/13);  total (15/13)

TAAGTGGTCACAAACGTACCGTTGCGGAACAGGTCCGCCATCAGATAGTTGCCTGCCAGCAGGGCTTCGAGGCTGTAGTTGTGGCCTTTGGCTTGCAGGATTTTATCTTCTTCGATTTTACCCAACTGGCTGATAACGCCATCGGCAGGCATGACCAGTACATTCGGATCGGTATCGATTGGGCGTACTTCGTCACGCAGCGGACGGACAAAGAATTCGTTAAAGGTGCGGTAGCTGGCGGTGTCCGGCTTTTGCGCCTCTTTCATGTCGA  >  NC_000913/4389951‑4390221                                                                                                                                        |                                                                                                                                        tAAGTGGTCACAAACGTACCGTTGCGGAACAGGTCCGCCATCAGATAGTTGCCTGCCAGCAGGGCTTCGAGGCTGTAGTTGTGGCCTTTGGCTTGCAGGATTTTATCTTCTTCGATTTTACCCAACTGGTTGATAGCGcc                                                                                                                                     <  1:185128/140‑1 (MQ=255) tAAGTGGTCACAAACGTACCGTTGCGGAACAGGTCCGCCATCAGATAGTTGCCTGCCAGCAGGGCTTCGAGGCTGTAGTTGTGGCCTTTGGCTTGCAGGATTTTATCTTCTTCGATTTTACCCAACTGGCTGATAGCGcc                                                                                                                                     <  2:128489/140‑1 (MQ=255)    gTGGTCACAAACGTACCGTTGCGGAACAGGTCCGCCATCAGATAGTTGCCTGCCAGCAGGGCTTCGAGGCTGTAGTTGTGGCCTTTGGCTTGCAGGATTTTATCTTCTTCGATTTTACCCAACTGGCTGATAGCGCCATc                                                                                                                                  >  2:249222/1‑140 (MQ=255)      ggTCACAAACGTACCGTTGCGGAACAGGTCCGCCATCAGATAGTTGCCTGCCAGCAGGGCTTCGAGGCTGTAGTTGTGGCCTTTGGCTTGCAGGATTTTATCTTCTTCGATTTTACCCAACTGGCTGATAGCGCCATCgg                                                                                                                                <  1:64274/140‑1 (MQ=255)        tCACAAACGTACCGTTGCGGAACAGGTCCGCCATCAGATAGTTGCCTGCCAGCAGGGCTTCGAGGCTGTAGTTGTGGCCTTTGGCTTGCAGGATTTTATCTTCTTCGATTTTACCCAACTGGCTGATAGCGcc                                                                                                                                     <  2:62828/133‑1 (MQ=255)        tCACAAACGTACCGTTGCGGAACAGGTCCGCCATCAGATAGTTGCCTGCCAGCAGGGCTTCGAGGCTGTAGTTGTGGCCTTTGGCTTGCAGGATTTTATCTTCTTCGATTTTACCCAACTGGCTGATAGCGcc                                                                                                                                     >  1:62828/1‑133 (MQ=255)        tCACAAACGTACCGTTGCGGAACAGGTCCGCCATCAGATAGTTGCCTGCCAGCAGGGCTTCGAGGCTGTAGTTGTGGCCTTTGGCTTGCAGGATTTTATCTTCTTCGATTTTACCCAACTGGCTGATAGCGCCATCggca                                                                                                                              >  2:273425/1‑140 (MQ=255)             aaCGTACCGTTGCGGAACAGGTCCGCCATCAGATAGTTGCCTGCCAGCAGGGCTTCGAGGCTGTAGTTGTGGCCTTTGGCTTGCAGGATTTTATCTTCTTCGATTTTACCCAACTGGCTGATAGCGCCATCGGCAGGCAt                                                                                                                         <  1:96940/140‑1 (MQ=255)                  aCCGTTGCGGAACAGGTCCGCCATCAGATAGTTGCCTGCCAGCAGGGCTTCGAGGCTGTAGTTGTGGCCTTTGGCTTGCAGGATTTTATCTTCTTCGATTTTACCCAACTGGCTGATAGCGCCATCGGCAGGCATGACCa                                                                                                                    >  2:278808/1‑140 (MQ=255)                         cGGAACAGGTCCGCCATCAGATAGTTGCCTGCCAGCAGGGCTTCGAGGCTGTAGTTGTGGCCTTTGGCTTGCAGGATTTTATCTTCTTCGATTTTACCCAACTGGCTGATAGCGCCATCGGCAGGCATGACCAGTACAtt                                                                                                             <  1:249222/140‑1 (MQ=255)                                 gTCCGCCATCAGATAGTTGCCTGCCAGCAGGGCTTCGAGGCTGTAGTTGTGGCCTTTGGCTTGCAGGATTTTATCTTCTTCGATTTTACCCAACTGGCTGATAGCGCCATCGGCAGGCATGACCAGTACATTCGGATCgg                                                                                                     >  1:276438/1‑140 (MQ=255)                                        aTCAGATAGTTGCCTGCCAGCAGGGCTTCGAGGCTGTAGTTGTGGCCTTTGGCTTGCAGGATTTTATCTTCTTCGATTTTACCCAACTGGCTGATAGCGCCATCGGCAGGCATGACCAGTACATTCGGATCGGTATCGAt                                                                                              <  1:278808/140‑1 (MQ=255)                                           aGATAGTTGCCTGCCAGCAGGGCTTCGAGGCTGTAGTTGTGGCCTTTGGCTTGCAGGATTTTATCTTCTTCGATTTTACCCAACTGGCTGATAGCGCCATCGGCAGGCATGACCAGTACATTCGGATCGGt                                                                                                    <  1:316933/131‑1 (MQ=255)                                           aGATAGTTGCCTGCCAGCAGGGCTTCGAGGCTGTAGTTGTGGCCTTTGGCTTGCAGGATTTTATCTTCTTCGATTTTACCCAACTGGCTGATAGCGCCATCGGCAGGCATGACCAGTACATTCGGATCGGt                                                                                                    >  2:316933/1‑131 (MQ=255)                                                 ttGCCTGCCAGCAGGGCTTCGAGGCTGTAGTTGTGGCCTTTGGCTTGCAGGATTTTATCTTCTTCGATTTTACCCAACTGGCTGATAGCGCCATCGGCAGGCATGACCAGTACATTCGGATCGGTATCGATTGGGCGTAc                                                                                     >  2:123763/1‑140 (MQ=255)                                                 ttGCCTGCCAGCAGGGCTTCGAGGCTGTAGTTGTGGCCTTTGGCTTGCAGGATTTTATCTTCTTCGATTTTACCCAACTGGCTGATAGCGCCATCGGCAGGCATGACCAGTACATTCGGATCGGTATCGATTGGGCGTAc                                                                                     >  1:162125/1‑140 (MQ=255)                                                   gcctgccAGCAGGGCTTCGAGGCTGTAGTTGTGGCCTTTGGCTTGCAGGATTTTATCTTCTTCGATTTTACCCAACTGGCTGATAGCGCCATCGGCAGGCATGACCAGTACATTCGGATCGGTATCGATTGGGCGTACtt                                                                                   >  1:155958/1‑140 (MQ=255)                                                             agGGCTTCGAGGCTGTAGTTGTGGCCTTTGGCTTGCAGGATTTTATCTTCTTCGATTTTACCCAACTGGCTGATAGCGCCATCGGCAGGCATGACCAGTACATTCGGATCGGTATCGATTGGGCGTACTTCGTCACGCAg                                                                         <  1:273425/140‑1 (MQ=255)                                                                      aGGCTGTAGTTGTGGCCTTTGGCTTGCAGGATTTTATCTTCTTCGATTTTACCCAACTGGCTGATAGCGCCATCGGCAGGCATGACCAGTACATTCGGATCGGTATCGATTGGGCGTACTTCGTCACGCAGCggacggac                                                                >  1:123987/1‑140 (MQ=255)                                                                                    gCCTTTGGCTTGCAGGATTTTATCTTCTTCGATTTTACCCAACTGGCTGATAGCGCCATCGGCAGGCATGACCAGTACATTCGGATCGGTATCGATTGGGCGTACTTCGTCACGCAGCGGACGGACAAAGAATTCGTTaa                                                  >  2:268148/1‑140 (MQ=255)                                                                                         tGGCTTGCAGGATTTTATCTTCTTCGATTTTACCCAACTGGCTGATAGCGCCATCGGCAGGCATGACCAGTACATTCGGATCGGTATCGGTTGGGCGTACTTCGTCACGCAGCGGACGGACAAAGAATTCGTTAAAGGTg                                             >  1:250167/1‑140 (MQ=255)                                                                                         tGGCTTGCAGGATTTTATCTTCTTCGATTTTACCCAACTGGCTGATAGCGCCATCGGCAGGCATGACCAGTACATTCGGATCGGTATCGATTGGGCGTACTTCGTCACGCAGCGGACGGACAAAGAATTCGTTAAAGGTg                                             >  2:186874/1‑140 (MQ=255)                                                                                                    aTTTTATCTTCTTCGATTTTACCCAACTGGCTGATAGCGCCATCGGCAGGCATGACCAGTACATTCGGATCGGTATCGATTGGGCGTACTTCGTCACGCAGCGGACGGACAAAGAATTCGTTAAAGGTGCGGTAGCTGGc                                  <  2:162125/140‑1 (MQ=255)                                                                                                               ttcGATTTTACCCAACTGGCTGATAGCGCCATCGGCAGGCATGACCAGTACATTCGGATCGGTATCGATTGGGCGTACTTCGTCACGCAGCGGACGGACAAAGAATTCGTTAAAGGTGCGGTAGCTGGCGGTGTCCGGCt                       >  1:130941/1‑140 (MQ=255)                                                                                                                      ttACCCAACTGGCTGATAGCGCCATCGGCAGGCATGACCAGTACATTCGGATCGGTATCGATTGGGCGTACTTCGTCACGCAGCGGACGGACAAAGAATTCGTTAAAGGTGCGGTAGCTGGCGGTGTCCGGCTTTTgcgc                <  2:130941/140‑1 (MQ=255)                                                                                                                          ccAACTGGCTGATAGCGCCATCGGCAGGCATGACCAGTACATTCGGATCGGTATCGATTGGGCGTACTTCGTCACGCAGCGGACGGACAAAGAATTCGTTAAAGGTGCGGTAGCTGGCGGTGTCCGGCTTTTGCGCctct            <  2:123987/140‑1 (MQ=255)                                                                                                                              cTGGCTGATAGCGCCATCGGCAGGCATGACCAGTACATTCGGATCGGTATCGATTGGGCGTACTTCGTCACGCAGCGGACGGACAAAGAATTCGTTAAAGGTGCGGTAGCTGGCGGTGTCCGGCTTTTGCGCCTCTTTCa        >  1:209585/1‑140 (MQ=255)                                                                                                                                    gATAGCGCCATCGGCAGGCATGACCAGTACATTCGGATCGGTATCGATTGGGCGTACTTCGTCACGCAGCGGACGGACAAAGAATTCGTTAAAGGTGCGGTAGCTGGCGGTGTCCGGCTTTTGCGCCTCTTTCATGTCGa  <  2:107242/140‑1 (MQ=255)                                                                                                                                        |                                                                                                                                        TAAGTGGTCACAAACGTACCGTTGCGGAACAGGTCCGCCATCAGATAGTTGCCTGCCAGCAGGGCTTCGAGGCTGTAGTTGTGGCCTTTGGCTTGCAGGATTTTATCTTCTTCGATTTTACCCAACTGGCTGATAACGCCATCGGCAGGCATGACCAGTACATTCGGATCGGTATCGATTGGGCGTACTTCGTCACGCAGCGGACGGACAAAGAATTCGTTAAAGGTGCGGTAGCTGGCGGTGTCCGGCTTTTGCGCCTCTTTCATGTCGA  >  NC_000913/4389951‑4390221

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑