Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,422,530	T→C	G115G (GGT→GGC)	ulaD →	3‑keto‑L‑gulonate‑6‑phosphate decarboxylase UlaD

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,422,530	0	T	C	100.0%	56.2 / NA	17	G115G (GGT→GGC)	ulaD	3‑keto‑L‑gulonate‑6‑phosphate decarboxylase UlaD
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (7/10);  total (7/10)

AATCCTTTCGCGTATGTGCTTCGAAGCCAACGCTGACTGGGTGACGGTAATTTGCTGTGCGGATATCAACACCGCCAAAGGCGCGCTGGACGTGGCAAAAGAGTTTAACGGCGACGTGCAGATCGAACTGACCGGTTACTGGACCTGGGAACAGGCGCAACAGTGGCGCGATGCAGGCATTGGGCAGGTGGTTTATCACCGCAGCCGTGACGCGCAGGCCGCAGGCGTGGCGTGGGGCGAAGCGGACATCACCGCG  >  NC_000913/4422395‑4422650                                                                                                                                        |                                                                                                                         aaTCCTTTCGCGTATGTGCTTCGAAGCCAACGCTGACTGGGTGACGGTAATTTGCTGTGCGGATATCAACACCGCCAAAGGCGCGCTGGACGTGGCAAAAGAGTTTAACGGCGACGTGCAGATCGAACTGACCGGCTACt                                                                                                                      <  2:93155/140‑1 (MQ=255)                              aCGCTGACTGGGTGACGGTAATTTGCTGTGCGGATATCAACACCGCCAAAGGCGCGCTGGACGTGGCAAAAGAGTTTAACGGCGACGTGCAGATCGAACTGACCGGCTACTGGACCTGGGAACAGGCGCAACAGTGgcgc                                                                                         <  2:304346/140‑1 (MQ=255)                                         gTGACGGTAATTTGCTGTGCGGATATCAACACCGCCAAAGGCGCGCTGGACGTGGCAAAAGAGTTTAACGGCGACGTGCAGATCGAACTGACCGGCTACTGGACCTGGGAACAGGCGCAACAGTGGCGCGATGc                                                                                    <  2:155059/134‑1 (MQ=255)                                         gTGACGGTAATTTGCTGTGCGGATATCAACACCGCCAAAGGCGCGCTGGACGTGGCAAAAGAGTTTAACGGCGACGTGCAGATCGAACTGACCGGCTACTGGACCTGGGAACAGGCGCAACAGTGGCGCGATGc                                                                                    >  1:155059/1‑134 (MQ=255)                                              ggTAATTTGCTGTGCGGATATCAACACCGCCAAAGGCGCGCTGGACGTGGCAAAAGAGTTTAACGGCGACGTGCAGATCGAACTGACCGGCTACTGGACCTGGGAACAGGCGCAACAGTGGCGCGATGCAGGCATTGGGc                                                                         >  1:74532/1‑140 (MQ=255)                                               gTAATTTGCTGTGCGGATATCAACACCGCCAAAGGCGCGCTGGACGTGGCAAAAGAGTTTAACGGCGACGTGCAGATCGAACTGACCGGCTACTGGACCTGGGAACAGGCGCAACAGTGGCGCGATGCAGGCATTGGGCa                                                                        <  1:91944/140‑1 (MQ=255)                                                 aaTTTGCTGTGCGGATATCAACACCGCCAAAGGCGCGCTGGACGTGGCAAAAGAGTTTAACGGCGACGTGCAGATCGAACTGACCGGCTACTGGACCTGGGAACAGGCGCAACAGTGGCGCGATGCAGGCATTGGGCAgg                                                                      <  2:74532/140‑1 (MQ=255)                                                          tgCGGATATCAACACCGCCAAAGGCGCGCTGGACGTGGCAAAAGAGTTTAACGGCGACGTGCAGATCGAACTGACCGGCTACTGGACCTGGGAACAGGCGCAACAGTGGCGCGATGCAGGCATTGGGCAGGTGGTTTATc                                                             >  2:145818/1‑140 (MQ=255)                                                            cGGATATCAACACCGCCAAAGGCGCGCTGGACGTGGCAAAAGAGTTTAACGGCGACGTGCAGATCGAACTGACCGGCTACTGGACCTGGGAACAGGCGCAACAGTGGCGCGATGCAGGCATTGGGCAgg                                                                      <  2:295358/129‑1 (MQ=255)                                                            cGGATATCAACACCGCCAAAGGCGCGCTGGACGTGGCAAAAGAGTTTAACGGCGACGTGCAGATCGAACTGACCGGCTACTGGACCTGGGAACAGGCGCAACAGTGGCGCGATGCAGGCATTGGGCAgg                                                                      >  1:295358/1‑129 (MQ=255)                                                                          gCCAAAGGCGCGCTGGACGTGGCAAAAGAGTTTAACGGCGACGTGCAGATCGAGCTGACCGGCTACTGGACCTGGGAACAGGCGCAACAGTGGCGCGATGCAGGCATTGGGCAGGTGGTTTATCACCGCAGCCGTGAcgc                                             >  1:40019/1‑140 (MQ=255)                                                                           ccAAAGGCGCGCTGGACGTGGCAAAAGAGTTTAACGGCGACGTGCAGATCGAACTGACCGGCTACTGGACCTGGGAACAGGCGCAACAGTGGCGCGATGCAGGCATTGGGCAGGTGGTTTATCACCGCAGCCGTGAcgcg                                            >  2:319640/1‑140 (MQ=255)                                                                                  cgcgcTGGACGTGGCAAAAGAGTTTAACGGCGACGTGCAGATCGAACTGACCGGCTACTGGACCTGGGAACAGGCGCAACAGTGGCGCGATGCAGGCATTGGGCAGGTGGTTTATCACCGCAGCCGTGACGcgcaggccg                                     <  2:116864/140‑1 (MQ=255)                                                                                                    agagTTTAACGGCGACGTGCAGATCGAACTGACCGGCTACTGGACCTGGGAACAGGCGCAACAGTGGCGCGATGCAGGCATTGGGCAGGTGGTTTATCACCGCAGCCGTGACGCGCAGGCCGCAGGCGTGGCGTGGGGCg                   <  2:285456/140‑1 (MQ=255)                                                                                                        tttAACGGCGACGTGCAGATCGAACTGACCGGCTACTGGACCTGGGAACAGGCGCAACAGTGGCGCGATGCAGGCATTGGGCAGGTGGTTTATCACCGCAGCCGTGACGCGCAGGCCGCAGGCGTGGCGTGGGGCGAAGc               >  1:42551/1‑140 (MQ=255)                                                                                                            aCGGCGACGTGCAGATCGAACTGACCGGCTACTGGACCTGGGAACAGGCGCAACAGTGGCGCGATGCAGGCATTGGGCAGGTGGTTTATCACCGCAGCCGTGACGCGCAGGCCGCAGGCGTGGCGTGGGGCGAAGCGGAc           <  1:319640/140‑1 (MQ=255)                                                                                                                     tGCAGATCGAGCTGACCGGCTACTGGACCTGGGAACAGGCGCAACAGTGGCGCGATGCAGGCATTGGGCAGGTGGTTTATCACCGCAGCCGTGACGCGCAGGCCGCAGGCGTGGCGTGGGGCGAAGCGGACATCACcgcg  <  2:40019/140‑1 (MQ=255)                                                                                                                                        |                                                                                                                         AATCCTTTCGCGTATGTGCTTCGAAGCCAACGCTGACTGGGTGACGGTAATTTGCTGTGCGGATATCAACACCGCCAAAGGCGCGCTGGACGTGGCAAAAGAGTTTAACGGCGACGTGCAGATCGAACTGACCGGTTACTGGACCTGGGAACAGGCGCAACAGTGGCGCGATGCAGGCATTGGGCAGGTGGTTTATCACCGCAGCCGTGACGCGCAGGCCGCAGGCGTGGCGTGGGGCGAAGCGGACATCACCGCG  >  NC_000913/4422395‑4422650

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑