Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,430,919	T→C	C352C (TGT→TGC)	cycA →	serine/alanine/glycine/cycloserine:H(+)symporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,430,919	0	T	C	100.0%	48.9 / NA	15	C352C (TGT→TGC)	cycA	serine/alanine/glycine/cycloserine:H(+)symporter
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (8/7);  total (8/7)

TCTTCCGCTAACAGCGGCGTCTTCTCTACCAGCCGTATGCTGTTTGGTCTGGCGCAGGAAGGTGTGGCACCGAAAGCGTTCGCTAAACTTTCTAAGCGCGCAGTACCCGCGAAAGGGCTGACGTTCTCGTGTATCTGTCTGCTCGGCGGCGTGGTGATGTTGTATGTGAATCCTAGTGTGATTGGCGCGTTCACGATGATTACAACCGTTTCCGCGATTCTGTTTATGTTCGTCTGGACGATTATCCTTTGCTC  >  NC_000913/4430788‑4431041                                                                                                                                    |                                                                                                                           tCTTCCGCTAACAGCGGCGTCTTCTCTACCAGCCGTATGCTGTTTGGTCTGGCGCAGGAAGGTGTGGCACCGAAAGCGTTCGCTAAACTTTCTAAGCGCGCAGTACCCGCGAAAGGGCTGACGTTCTCGTGCAtctgtct                                                                                                                    >  2:25850/1‑140 (MQ=255)            cAGCGGCGTCTTCTCTACCAGCCGTATGCTGTTTGGTCTGGCGCAGGAAGGTGTGGCACCGAAAGCGTTCGCTAAACTTTCTAAGCGCGCAGTACCCGCGAAAGGGCTGACGTTCTCGTGCATCTGTCTGCTCGgcggcg                                                                                                         >  2:333004/1‑140 (MQ=255)              gcggcgTCTTCTCTACCAGCCGTATGCTGTTTGGTCTGGCGCAGGAAGGTGTGGCACCGAAAGCGTTCGCTAAACTTTCTAAGCGCGCAGTACCCGCGAAAGGGCTGACGTTCTCGTGCATCTGTCTGCTCGGCGGCgtg                                                                                                       <  2:104533/140‑1 (MQ=255)                            aCCAGCCGTATGCTGTTTGGTCTGGCGCAGGAAGGTGTGGCACCGAAAGCGTTCGCTAAACTTTCTAAGCGCGCAGTACCCGCGAAAGGGCTGACGTTCTCGTGCATCTGTCTGCTCGGCGGCGTGGTGATGTTGTAtgt                                                                                         <  1:333004/140‑1 (MQ=255)                            aCCAGCCGTATGCTGTTTGGTCTGGCGCAGGAAGGTGTGGCACCGAAAGCGTTCGCTAAACTTTCTAAGCGCGCAGTACCCGCGAAAGGGCTGACGTTCTCGTGCATCTGTCTGCTCGGCGGCGTGGTGATGTTGTAtgt                                                                                         <  2:274/140‑1 (MQ=255)                                 ccGTATGCTGTTTGGTCTGGCGCAGGAAGGTGTGGCACCGAAAGCGTTCGCTAAACTTTCTAAGCGCGCAGTACCCGCGAAAGGGCTGACGTTCTCGTGCATCTGTCTGCTCGGCGGCGTGGTGATGTTGTATGTGAATc                                                                                    >  1:84524/1‑140 (MQ=255)                                     aTGCTGTTTGGTCTGGCGCAGGAAGGTGTGGCACCGAAAGCGTTCGCTAAACTTTCTAAGCGCGCAGTACCCGCGAAAGGGCTGACGTTCTCGTGCATCTGTCTGCTCGGCGGCGTGGTGATGTTGTATGTGAATCCTAg                                                                                >  2:119172/1‑140 (MQ=255)                                                               tgtgGCACCGAAAGCGTTCGCTAAACTTTCTAAGCGCGCAGTACCCGCGAAAGGGCTGACGTTCTCGTGCATCTGTCTGCTCGGCGGCGTGGTGATGTTGTATGTGAATCCTAGTGTGATTGGCGCGTTCACGATGATTa                                                      >  1:37237/1‑140 (MQ=255)                                                                    cACCGAAAGCGTTCGCTAAACTTTCTAAGCGCGCAGTACCCGCGAAAGGGCTGATGTTCTCGTGCATCTGTCTGCTCGGCGGCGTGGTGATGTTGTATGTGAATCCTAGTGTGATTGGCGCGTTCACGATGATTACAAcc                                                 >  1:95974/1‑140 (MQ=255)                                                                    cACCGAAAGCGTTCGCTAAACTTTCTAAGCGCGCAGTACCCGCGAAAGGGCTGACGTTCTCGTGCATCTGTCTGCTCGGCGGCGTGGTGATGTTGTATGTGAATCCTAGTGTGATTGGCGCGTTCACGATGATTACAAcc                                                 >  2:338806/1‑140 (MQ=255)                                                                                                   cgcAGTACCCGCGAAAGGGCTGACGTTCTCGTGCATCTGTCTGCTCGGCGGCGTGGTGATGTTGTATGTGAATCCTAGTGTGATTGGCGCGTTCACGATGATTACAACCGTTTCCGCGATTCTGTTTATGTTCGTCTGGa                  <  2:84524/140‑1 (MQ=255)                                                                                                       gTACCCGCGAAAGGGCTGACGTTCTCGTGCATCTGTCTGCTCGGCGGCGTGGTGATGTTGTATGTGAATCCTAGTGTGATTGGCGCGTTCACGATGATTACAACCGTTTCCGCGATTCTGTTTATGTTCGTCTGGACGAt              <  1:119172/140‑1 (MQ=255)                                                                                                                aaaGGGCTGACGTTCTCGTGCATCTGTCTGCTCGGCGGCGTGGTGATGTTGTATGTGAATCCTAGTGTGATTGGCGCGTTCACGATGATTACAACCGTTTCCGCGATTCTGTTTATGTTCGTCTGGACGATTATCCTTTg     >  1:130762/1‑140 (MQ=255)                                                                                                                   gggCTGACGTTCTCGTGCATCTGTCTGCTCGGCGGCGTGGTGATGTTGTATGTGAATCCTAGTGTGATTGGCGCGTTCACGATGATTACAACCGTTTCCGCGATTCTGTTTATGTTCGTCTGGACGATTATCCTTTGCTc  <  1:100158/140‑1 (MQ=255)                                                                                                                   gggCTGACGTTCTCGTGCATCTGTCTGCTCGGCGGCGTGGTGATGTTGTATGTGAATCCTAGTGTGATTGGCGCGTTCACGATGATTACAACCGTTTCCGCGATTCTGTTTATGTTCGTCTGGACGATTATCCTTTGCTc  <  1:225983/140‑1 (MQ=255)                                                                                                                                    |                                                                                                                           TCTTCCGCTAACAGCGGCGTCTTCTCTACCAGCCGTATGCTGTTTGGTCTGGCGCAGGAAGGTGTGGCACCGAAAGCGTTCGCTAAACTTTCTAAGCGCGCAGTACCCGCGAAAGGGCTGACGTTCTCGTGTATCTGTCTGCTCGGCGGCGTGGTGATGTTGTATGTGAATCCTAGTGTGATTGGCGCGTTCACGATGATTACAACCGTTTCCGCGATTCTGTTTATGTTCGTCTGGACGATTATCCTTTGCTC  >  NC_000913/4430788‑4431041

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑