Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,445,549	A→G	T480A (ACC→GCC)	tamB →	translocation and assembly module subunit TamB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,445,549	0	A	G	100.0%	107.6 / NA	31	T480A (ACC→GCC)	tamB	translocation and assembly module subunit TamB
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (11/20);  total (11/20)

ACAGGCCATTAGTTGGCGCGGTGAGCTAACGCTTAACGGCATTAACACCGCCAAAGAGTTCCCGGACTGGCCGTCGAAACTCAATGGCTTGATTAAAACCCGCGGTAGCCTGTACGGCGGCACCTGGCAGATGGACGTGCCGGAGTTGAAGCTGACCGGTAACGTTAAACAGAACAAAGTGAACGTTGACGGCACGCTGAAAGGCAACAGTTATATGCAGTGGATGATCCCAGGGCTTCATCTGGAACTCGGGCCAAA  >  NC_000913/4445428‑4445685                                                                                                                          |                                                                                                                                         aCAGGCCATTAGTTGGCGCGGTGAGCTAACGCTTAACGGCATTAACACCGCCAAAGAGTTCCCGGACTGGCCGTCGAAACTCAATGGCTTGATTAAAACCCGCGGTAGCCTGTACGGCGGCGCCTGGCAGATGGACGTGc                                                                                                                        <  2:92666/140‑1 (MQ=255)     gCCATTAGTTGGCGCGGTGAGCTAACGCTTAACGGCATTAACACCGCCAAAGAGTTCCCGGACTGGCCGTCGAAACTCAATGGCTTGATTAAAACCCGCGGTAGCCTGTACGGCGGCGCCTGGCAGATgg                                                                                                                              >  2:352777/1‑130 (MQ=255)     gCCATTAGTTGGCGCGGTGAGCTAACGCTTAACGGCATTAACACCGCCAAAGAGTTCCCGGACTGGCCGTCGAAACTCAATGGCTTGATTAAAACCCGCGGTAGCCTGTACGGCGGCGCCTGGCAGATgg                                                                                                                              <  1:352777/130‑1 (MQ=255)        aTTAGTTGGCGCGGTGAGCTAACGCTTAACGGCATTAACACCGCCAAAGAGTTCCCGGACTGGCCGTCGAAACTCAATGGCTTGATTAAAACCCGCGGTAGCCTGTACGGCGGCGCCTGGCAGATGGACGTGCCGGAGtt                                                                                                                 >  2:265042/1‑140 (MQ=255)                 cgcgGTGAGCTAACGCTTAACGGCATTAACACCGCCAAAGAGTTCCCGGACTGGCCGTCGAAACTCAATGGCTTGATTAAAACCCGCGGTAGCCTGTACGGCGGCGCCTGGCAGATGGACGTGCCGGAGTTGAAGCTGAc                                                                                                        <  2:222593/140‑1 (MQ=255)                 cgcgGTGAGCTAACGCTTAACGGCATTAACACCGCCAAAGAGTTCCCGGACTGGCCGTCGAAACTCAATGGCTTGATTAAAACCCGCGGTAGCCTGTACGGCGGCGCCTGGCAGATGGACGTGCCGGAGTTGAAGCTGAc                                                                                                        <  1:286512/140‑1 (MQ=255)                    ggTGAGCTAACGCTTAACGGCATTAACACCGCCAAAGAGTTCCCGGACTGGCCGTCGAAACTCAATGGCTTGATTAAAACCCGCGGTAGCCTGTACGGCGGCGCCTGGCAGATGGACGTGCCGGAGTTGAAGCTGACCgg                                                                                                     <  2:63408/140‑1 (MQ=255)                              cGCTTAACGGCATTAACACCGCCAAAGAGTTCCCGGACTGGCCGTCGAAACTCAATGGCTTGATTAAAACCCGCGGTAGCCTGTACGGCGGCGCCTGGCAGATGGACGTGCCGGAGTTGAAGCTGACCGGTAACGTTaaa                                                                                           >  2:253513/1‑140 (MQ=255)                               gCTTAACGGCATTAACACCGCCAAAGAGTTCCCGGACTGGCCGTCGAAACTCAATGGCTTGATTAAAACCCTCGGTAGCCTGTACGGCGGCGCCTGGCAGATGGACGTGCCGGAGTTGAAGCTGACCGGTAACGTTAAAc                                                                                          <  2:224345/140‑1 (MQ=255)                                cTTAACGGCATTAACACCGCCAAAGAGTTCCCGGACTGGCCGTCGAAACTCAATGGCTTGATTAAAACCCGCGGTAGCCTGTACGGCGGCGCCTGGCAGATGGACGTGCCGGAGTTGAAGCTGACCGATAACGTTAAACa                                                                                         >  2:260762/1‑140 (MQ=255)                                    aCGGCATTAACACCGCCAAAGAGTTCCCGGACTGGCCGTCGAAACTCAATGGCTTGATTAAAACCCGCGGTAGCCTGTATGGCGGCGCCTGGCAGATGGACGTGCCGGAGTTGAAGCTGACCGGTAACGTTAAACAGAAc                                                                                     >  2:164499/1‑140 (MQ=255)                                       gCATTAACACCGCCAAAGAGTTCCCGGACTGGCCGTCGAAACTCAATGGCTTGATTAAAACCCGCGGTAGCCTGTACGGCGGCGCCTGGCAGATGGACGTGCCGGAGTTGAAGCTGACCGGTAACGTTAAACAGAACaaa                                                                                  >  1:20473/1‑140 (MQ=255)                                         aTTAACACCGCCAAAGAGTTCCCGGACTGGCCGTCGAAACTCAATGGCTTGATTAAAACCCGCGGTAGCCTGTACGGCGGCGCCTGGCAGATGGACGTGCCGGAGTTGAAGCTGACCGGTAACGTTAAACAGAACAAAGt                                                                                >  1:176204/1‑140 (MQ=255)                                                    cAAAGAGTTCCCGGACTGGCCGTCGAAACTCAATGGCTTGATTAAAACCCGCGGTAGCCTGTACGGCGGCGCCTGGCAGATGGACGTGCCGGAGTTGAAGCTGACCGGTAACGTTAAACAGAACAAAGTGAACGTTGACg                                                                     <  2:176204/140‑1 (MQ=255)                                                                    tGGCCGTCGAAACTCAATGGCTTGATTAAAACCCGCGGTAGCCTGTACGGCGGCGCCTGGCAGATGGACGTGCCGGAGTTGAAGCTGACCGGTAACGTTAAACAGAACAAAGTGAACGTTGACGGCACGCTGAAAGGCaa                                                     <  1:333477/140‑1 (MQ=255)                                                                    tGGCCGTCGAAACTCAATGGCTTGATTAAAACCCGCGGTAGCCTGTACGGCGGCGCCTGGCAGATGGACGTGCCGGAGTTGAAGCTGACCGGTAACGTTAAACAGAACAAAGTGAACGTTGACGGCACGCTGAAAGGCaa                                                     <  1:103947/140‑1 (MQ=255)                                                                     ggCCGTCGAAACTCAATGGCTTGATTAAAACCCGCGGTAGCCTGTACGGCGGCGCCTGGCAGATGGACGTGCCGGAGTTGAAGCTGACCGGTAACGTTAAACAGAACAAAGTGAACGTTGACGGCACGCTGAAAGGCAAc                                                    >  2:98757/1‑140 (MQ=255)                                                                      gCCGTCGAAACTCAATGGCTTGATTAAAACCCGCGGTAGCCTGTATGGCGGCGCCTGGCAGATGGACGTGCCGGAGTTGAAGCTGACCGGTAACGTTAAACAGAACAAAGTGAACGTTGACGGCACGCTGAAAGGCAACa                                                   <  1:164499/140‑1 (MQ=255)                                                                        cGTCGAAACTCAATGGCTTGATTAAAACCCGCGGTAGCCTGTACGGCGGCGCCTGGCAGATGGACGTGCCGGAGTTGAAGCTGACCGGTAACGTTAAACAGAACAAAGTGAACGTTGACGGCACGCTGAAAGGCaa                                                     >  2:129319/1‑136 (MQ=255)                                                                        cGTCGAAACTCAATGGCTTGATTAAAACCCGCGGTAGCCTGTACGGCGGCGCCTGGCAGATGGACGTGCCGGAGTTGAAGCTGACCGGTAACGTTAAACAGAACAAAGTGAACGTTGACGGCACGCTGAAAGGCaa                                                     <  1:129319/136‑1 (MQ=255)                                                                         gTCGAAACTCAATGGCTTGATTAAAACCCGCGGTAGCCTGTACGGCGGCGCCTGGCAGATGGACGTGCCGGAGTTGAAGCTGACCGATAACGTTAAACAGAACAAAGTGAACGTTGACGGCACGCTGAAAGGCAACAGtt                                                <  1:260762/140‑1 (MQ=255)                                                                            gAAACTCAATGGCTTGATTAAAACCCGCGGTAGCCTGTACGGCGGCGCCTGGCAGATGGACGTGCCGGAGTTGAAGCTGACCGGTAACGTTAAACAGAACAAAGTGAACGTTGACGGCACGCTGAAAGGCAACAGTtata                                             <  1:321380/140‑1 (MQ=255)                                                                             aaaCTCAATGGCTTGATTAAAACCCGCGGTAGCCTGTACGGCGGCGCCTGGCAGATGGACGTGCCGGAGTTGAAGCTGACCGGTAACGTTAAACAGAACAAAGTGAACGTTGACGGCACGCTGAAAGGCAACAGTTatat                                            <  1:98757/140‑1 (MQ=255)                                                                                     tGGCTTGATTAAAACCCGCGGTAGCCTGTACGGCGGCGCCTGGCAGATGGACGTGCCGGAGTTGAAGCTGACCGGTAACGTTAAACAGAACAAAGTGAACGTTGACGGCACGCTGAAAGGCAACAGTTATATGCAGTGga                                    <  2:16054/140‑1 (MQ=255)                                                                                      ggCTTGATTAAAACCCGCGGTAGCCTGTACGGCGGCGCCTGGCAGATGGACGTGCCGGAGTTGAAGCTGACCGGTAACGTTAAACAGAACAAAGTGAACGTTGACGGCACGCTGAAAGGCAACAGTTATATGCAGTGgat                                   <  1:121591/140‑1 (MQ=255)                                                                                           gATTAAAACCCGCGGTAGCCTGTACGGCGGCGCCTGGCAGATGGACGTGCCGGAGTTGAAGCTGACCGGTAACGTTAAACAGAACAAAGTGAACGTTGACGGCACGCTGAAAGGCAACAGTTATATGCAGTGGATGATcc                              <  2:225466/140‑1 (MQ=255)                                                                                                             ccTGTACGGCGGCGCCTGGCAGATGGACGTGCCGGAGTTGAAGCTGACCGGTAACGTTAAACAGAACAAAGTGAACGTTGACGGCACGCTGAAAGGCAACAGTTATATGCAGTGGATGATCCCAGGGCTTCATCTGGAAc            <  1:253513/140‑1 (MQ=255)                                                                                                                  aCGGCGGCGCCTGGCAGATGGACGTGCCGGAGTTGAAGCTGACCGGTAACGTTAAACAGAACAAAGTGAACGTTGACGGCACGCTGAAAGGCAACAGTTATATGCAGTGGATGATCCCAGGGCTTCATCTGGAACTCggg       >  2:301360/1‑140 (MQ=255)                                                                                                                      cggcGCCTGGCAGATGGACGTGCCGGAGTTGAAGCTGACCGGTAACGTTAAACAGAACAAAGTGAACGTTGACGGCACGCTGAAAGGCAACAGTTATATGCAGTGGATGATCCCAGGGCTTCATCTGGAACTCGGGCCaa   >  1:223562/1‑140 (MQ=255)                                                                                                                       ggcGCCTGGCAGATGGACGTGCCGGAGTTGAAGCTGACCGGTAACGTTAAACAGAACAAAGTGAACGTTGACGGCACGCTGAAAGGCAACAGTTATATGCAGTGGATGATCCCAGGGCTTCATCTGGAACTCGGGCCaaa  <  2:231516/140‑1 (MQ=255)                                                                                                                       ggcGCCTGGCAGATGGACGTGCCGGAGTTGAAGCTGACCGGTAACGTTAAACAGAACAAAGTGAACGTTGACGGCACGCTGAAAGGCAACAGTTATATGCAGTGGATGATCCCAGGGCTTCATCTGGAACTCGGGCCaaa  <  2:20473/140‑1 (MQ=255)                                                                                                                          |                                                                                                                                         ACAGGCCATTAGTTGGCGCGGTGAGCTAACGCTTAACGGCATTAACACCGCCAAAGAGTTCCCGGACTGGCCGTCGAAACTCAATGGCTTGATTAAAACCCGCGGTAGCCTGTACGGCGGCACCTGGCAGATGGACGTGCCGGAGTTGAAGCTGACCGGTAACGTTAAACAGAACAAAGTGAACGTTGACGGCACGCTGAAAGGCAACAGTTATATGCAGTGGATGATCCCAGGGCTTCATCTGGAACTCGGGCCAAA  >  NC_000913/4445428‑4445685

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑