Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,522,746	A→G	V450A (GTG→GCG)	yjhG ←	KpLE2 phage‑like element; D‑xylonate dehydratase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,522,746	0	A	G	100.0%	109.7 / NA	32	V450A (GTG→GCG)	yjhG	KpLE2 phage‑like element; D‑xylonate dehydratase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (16/16);  total (16/16)

CGCCCGCCTTGATCTTGTCATGTTTGATATCGTAAATCGCACTTTTCTCGGACAGATAAACCTTCGCCACACCTTTATGGTAATAGATACCTTGCTCATCAATCATCGAGGGGTCAATGGCGGTGGATTTGATCACCGAACCTTCTGGCGCAATATTGCCCACCGGGAAGGTGATAGTTGAGGTTAATCCGCGCGCTTTTGCTTGCTGCGGAGACATGATCACTTCGTCAGCGTTGATTTGTTCCTGATCGAGCAGGAGTTGC  >  NC_000913/4522612‑4522874                                                                                                                                       |                                                                                                                                 cgcccgccTTGATCTTGTCATGTTTGATATCGTAAATCGCACTTTTCTCGGACAGATAAACCTTCGCCACACCTTTATGGTAATAGATACCTTGCTCATCAATCATCGAGGGGTCAATGGCGGTGGATTTGATCGCCGaa                                                                                                                             <  2:302245/140‑1 (MQ=255)      gccTTGATCTTGTCATGTTTGATATCGTAAATCGCACTTTTCTCGGACAGATAAACCTTCGCCACACCTTTATGGTAATAGATACCTTGCTCATCAATCATCGAGGGGTCAATGGCGGTGGATTTGATCGCCGAACCTTc                                                                                                                        <  2:237368/140‑1 (MQ=255)      gccTTGATCTTGTCATGTTTGATATCGTAAATCGCACTTTTCTCGGACAGATAAACCTTCGCCACACCTTTATGGTAATAGATACCTTGCTCATCAATCATCGAGGGGTCAATGGCGGTGGATTTGATCGCCGAACCTTc                                                                                                                        <  2:326079/140‑1 (MQ=255)           gATCTTGTCATGTTTGATATCGTAAATCGCACTTTTCTCGGACAGATAAACCTTCGCCACACCTTTATGGTAATAGATACCTTGCTCATCAATCATCGAGGGGTCAATGGCGGTGGATTTGATCGCCGAACCTTCTGgcg                                                                                                                   <  2:320283/140‑1 (MQ=255)                 gTCATGTTTGATATCGTAAATCGCACTTTTCTCGGACAGATAAACCTTCGCCACACCTTTATGGTAATAGATACCTTGCTCATCAATCATCGAGGGGTCAATGGCGGTGGATTTGATCGCCGAACCTTCTGGCACAatat                                                                                                             >  2:166/1‑140 (MQ=255)                      gTTTGATATCGTAAATCGCACTTTTCTCGGACAGATAAACCTTCGCCACACCTTTATGGTAATAGATACCTTGCTCATCAATCATCGAGGGGTCAATGGCGGTGGATTTGATCGCCGAACCTTCTGGCGCAATATTGcc                                                                                                         <  2:281819/139‑1 (MQ=255)                      gTTTGATATCGTAAATCGCACTTTTCTCGGACAGATAAACCTTCGCCACACCTTTATGGTAATAGATACCTTGCTCATCAATCATCGAGGGGTCAATGGCGGTGGATTTGATCGCCGAACCTTCTGGCGCAATATTGcc                                                                                                         >  1:281819/1‑139 (MQ=255)                         tGATATCGTAAATCGCACTTTTCTCGGACAGATAAACCTTCGCCACACCTTTATGGTAATAGATACCTTGCTCATCAATCATCGAGGGGTCAATGGCGGTGGATTTGATCGCCGAACCTTCTGGCGCAATATTGCCCAcc                                                                                                     <  2:109831/140‑1 (MQ=255)                           atatCGTAAATCGCACTTTTCTCGGACAGATAAACCTTCGCCACACCTTTATGGTAATAGATACCTTGCTCATCAATCATCGAGGGGTCAATGGCGGTGGATTTGATCGCCGAACCTTCTGGCGCAATATTGCCCACCgg                                                                                                   <  1:75971/140‑1 (MQ=255)                            tatCGTAAATCGCACTTTTCTCGGACAGATAAACCTTCGCCACACCTTTATGGTAATAGATACCTTGCTCATCAATCATCGAGGGGTCAATGGCGGTGGATTTGATCGCCGAACCTTCTGGCGCAATATTGCCCACCggg                                                                                                  >  2:265633/1‑140 (MQ=255)                                  aaaTCGCACTTTTCTCGGACAGATAAACCTTCGCCACACCTTTATGGTAATAGATACCTTGCTCATCAATCATCGAGGGGTCAATGGCGGTGGATTTGATCGCCGAACCTTCTGGCACAATATTGCCCACCGGGAAGGTg                                                                                            <  1:166/140‑1 (MQ=255)                                   aaTCGCACTTTTCTCGGACAGATAAACCTTCGCCACACCTTTATGGTAATAGATACCTTGCTCATCAATCATCGAGGGGTCAATGGCGGTGGATTTGATCGCCGAACCTTCTGGCGCAATATTGCCCACCGGGAAGGGGa                                                                                           >  2:268193/1‑140 (MQ=255)                                      cGCACTTTTCTCGGACAGATAAACCTTCGCCACACCTTTATGGTAATAGATACCTTGCTCATCAATCATCGAGGGGTCAATGGCGGTGGATTTGATCGCCGAACCTTCTGGCGCAATATTGCCCACCGGGAAGGTGATAg                                                                                        >  2:306312/1‑140 (MQ=255)                                                        aTAAACCTTCGCCACACCTTTATGGTAATAGATACCTTGCTCATCAATCATCGAGGGGTCAATGGCGGTGGATTTGATCGCCGAACCTTCTGGCGCAATATTGCCCACCGGGAAGGTGATAGTTGAGGTTAATCcgcgcg                                                                      <  1:306312/140‑1 (MQ=255)                                                                       acCTTTATGGTAATAGATACCTTGCTCATCAATCATCGAGGGGTCAATGGCGGTGGATTTGATCGCCGAACCTTCTGGCGCAATATTGCCCACCGGGAAGGTGATAGTTGAGGTTAATCCGCGCGCTTTTGCTTGCTGCg                                                       <  1:355308/140‑1 (MQ=255)                                                                           ttATGGTAATAGATACCTTGCTCATCAATCATCGAGGGGTCAATGGCGGTGGATTTGATCGCCGAACCTTCTGGCGCAATATTGCCCACCGGGAAGGTGATAGTTGAGGTTAATCCGCGCGCTTTTGCTTGCTGCGgaga                                                   >  1:29470/1‑140 (MQ=255)                                                                                           cTTGCTCATCAATCATCGAGGGGTCAATGGCGGTGGATTTGATCGCCGAACCTTCTGGCGCAATATTGCCCACCGGGAAGGTGATAGTTGAGGTTAATCCGCGCGCTTTTGCTTGCTGCGGAGACATGATCACTTCGTCa                                   >  1:317912/1‑140 (MQ=255)                                                                                           cTTGCTCATCAATCATCGAGGGGTCAATGGCGGTGGATTTGATCGCCGAACCTTCTGGCGCAATATTGCCCACCGGGAAGGTGATAGTTGAGGTTAATCCGCGCGCTTTTGCTTGCTGCGGAGACATGATCACTTCGTCa                                   <  1:137758/140‑1 (MQ=255)                                                                                             tGCTCATCAATCTTCGAGGGGTCAATGGCGGTGGATTTGATCGCCGAACCTTCTGGCGCAATATTGCCCACCGGGGAGGTGATAGTTGAGGTTAATCCGCGCGCTTTTGCTTGCTGCGGAGACATGATCACTTCGTCAGc                                 >  2:351932/1‑140 (MQ=255)                                                                                             tGCTCATCAATCATCGAGGGGTCAATGGCGGTGGATTTGATCGCCGAACCTTCTGGCGCAATATTGCCCACCGGGAAGGTGATAGTTGAGGTTAATCCGCGCGCTTTTGCTTGCTGCGGAGACATGATCACTTCGt                                     <  1:70884/136‑1 (MQ=255)                                                                                             tGCTCATCAATCATCGAGGGGTCAATGGCGGTGGATTTGATCGCCGAACCTTCTGGCGCAATATTGCCCACCGGGAAGGTGATAGTTGAGGTTAATCCGCGCGCTTTTGCTTGCTGCGGAGACATGATCACTTCGt                                     >  2:70884/1‑136 (MQ=255)                                                                                               cTCATCAATCATCGAGGGGTCAATGGCGGTGGATTTGATCGCCGAACCTTCTGGCGCAATATTGCCCACCGGGAAGGTGATAGTTGAGGTTAATCCGCGCGCTTTTGCTTGCTGCGGAGACATGATCACTTCGTCAGCGt                               >  2:7547/1‑140 (MQ=255)                                                                                                  atcaatcaTCGAGGGGTCAATGGCGGTGGATTTGATCGCCGAACCTTCTGGCGCAATATTGCCCACCGGGAAGGTGATAGTTGAGGGTAATCCGCGCGCTTTTGCTTGCTGCGGAGACATGATCACTTCGTCAGCGTTGa                            >  1:324175/1‑140 (MQ=255)                                                                                                             aGGGGTCAATGGCGGTGGATTTGATCGCCGAACCTTCTGGCGCAATATTGCCCACCGGGAAGGTGATAGTTGAGGTTAATCCGCGCGCTTTTGCTTGCTGCGGAGACATGATCACTTCGTCAGCGTTGAt                           <  2:186936/130‑1 (MQ=255)                                                                                                             aGGGGTCAATGGCGGTGGATTTGATCGCCGAACCTTCTGGCGCAATATTGCCCACCGGGAAGGTGATAGTTGAGGTTAATCCGCGCGCTTTTGCTTGCTGCGGAGACATGATCACTTCGTCAGCGTTGAt                           >  1:186936/1‑130 (MQ=255)                                                                                                              ggggTCAATGGCGGTGGATTTGATCGCCGAACCTTCTGGCGCAATATTGCCCACCGGGAAGGTGATAGTTGAGGTTAATCCGCGCGCTTTTGCTTGCTGCGGAGACATGATCACTTCGTCAGCGTTGATTTGTTCCTGAt                <  2:29470/140‑1 (MQ=255)                                                                                                                 gTCAATGGCGGTGGATTTGATCGCCGAACCTTCTGGCGCAATATTGCCCACCGGGAAGGTGATAGTTGAGGTTAATCCGCGCGCTTTTGCTTGCTGCGGAGACATGATCACTTCGTCAGCGTTGATTTGTTCCTGATCGa             >  2:344250/1‑140 (MQ=255)                                                                                                                     aTGGCGGTGGATTTGATCGCCGAACCTTCTGGCGCAATATTGCCCACCGGGAAGGTGATAGTTGAGGTTAATCCGCGCGCTTTTGCTTGCTGCGGAGACATGATCACTTCGTCAGCGTTGATTTGTTCCTGATCGAGCAg         >  1:35552/1‑140 (MQ=255)                                                                                                                        gCGGTGGATTTGATCGCCGAACCTTCTGGCGCAATATTGCCCACCGGGAAGGTGATAGTTGAGGTTAATCCGCGCGCTTTTGCTTGCTGCGGAGACATGATCACTTCGTCAGCGTTGATTTGTTCCTGATCGAGCAGGAg      <  1:298090/140‑1 (MQ=255)                                                                                                                          ggTGGATTTGATCGCCGAACCTTCTGGCGCAATATTGCCCACCGGGAAGGTGATAGTTGAGGTTAATCCGCGCGCTTTTGCTTGCTGCGGAGACATGATCACTTCGTCAGCGTTGATTTGTTCCTGATCGAGCAGGAGtt    <  2:35552/140‑1 (MQ=255)                                                                                                                           gTGGATTTGATCGCCGAACCTTCTGGCGCAATATTGCCCACCGGGAAGGTGATAGTTGAGGTTAATCCGCGCGCTTTTGCTTGCTGCGGAGACATGATCACTTCGTCAGCGTTGATTTGTTCCTGATCGAGCAGGAGTTg   >  2:220847/1‑140 (MQ=255)                                                                                                                            tGGATTTGATCGCCGAACCTTCTGGCGCAATATTGCCCACCGGGAAGGTGATAGTTGAGGTTAATCCGCGCGCTTTTGCTTGCTGCGGAGACATGATCACTTCGTCAGCGTTGATTTGTTCCTGATCGAGCAGGAGTTGc  >  2:111002/1‑140 (MQ=255)                                                                                                                                       |                                                                                                                                 CGCCCGCCTTGATCTTGTCATGTTTGATATCGTAAATCGCACTTTTCTCGGACAGATAAACCTTCGCCACACCTTTATGGTAATAGATACCTTGCTCATCAATCATCGAGGGGTCAATGGCGGTGGATTTGATCACCGAACCTTCTGGCGCAATATTGCCCACCGGGAAGGTGATAGTTGAGGTTAATCCGCGCGCTTTTGCTTGCTGCGGAGACATGATCACTTCGTCAGCGTTGATTTGTTCCTGATCGAGCAGGAGTTGC  >  NC_000913/4522612‑4522874

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑