Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	650,305	C→T	A62A (GCG→GCA)	citE ←	citrate lyase beta subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	650,305	0	C	T	100.0%	49.7 / NA	16	A62A (GCG→GCA)	citE	citrate lyase beta subunit
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (8/8);  total (8/8)

ACGCACAACGTCCGCACCACCGCGAACGACGGCTTCCAGGTCGTTAACACCCCATTCGGAATCCAGCGCGTTGACACGCACAATGGTTTCAATATCGCGATACAGCGGATGTTGCAGCGCGTGGTAAACCATGCGGCGGGCGGTGTCTTTTTCACGCAATGCTACGGAGTCTTCGAGGTCAAACATCAGGGCATCAGCCGGGTAGATGAAGGAGTTGCTGACCATCGCGGCATTGGCACCAGGCACAA  >  NC_000913/650188‑650435                                                                                                                      |                                                                                                                                   aCGCACAACGTCCGCACCACCGCGAACGACGGCTTCCAGGTCGTTAACACCCCATTCGGAATCCAGCGCGTTGACACGCACAATGGTTTCAATATCGCGATACAGCGGATGTTGCAGTGCGTGGTAAACCATGCggcggg                                                                                                              >  2:265394/1‑140 (MQ=255) aCGCACAACGTCCGCACCACCGCGAACGACGGCTTCCAGGTCGTTAACACCCCATTCGGAATCCAGCGCGTTGACACGCACAATGGTTTCAATATCGCGATACAGCGGATGTTGCAGTGCGTGGTAAACCATGCggcggg                                                                                                              >  2:80023/1‑140 (MQ=255)  cGCACAACGTCCGCACCACCGCGAACGACGGCTTCCAGGTCGTTAACACCCCATTCGGAATCCAGCGCGTTGACACGCACAATGGTTTCAATATCGCGATACAGCGGATGTTGCAGTGCGTGGTAAACCATGCggcgggc                                                                                                             <  1:193661/140‑1 (MQ=255)   gCACAACGTCCGCACCACCGCGAACGACGGCTTCCAGGTCGTTAACACCCCATTCGGAATCCAGCGCGTTGACACGCACAATGGTTTCAATATCGCGATACAGCGGATGTTGCAGTGCGTGGTAAACCATGCggcgggcg                                                                                                            <  1:265394/140‑1 (MQ=255)                             acgGCTTCCAGGTCGTTAACACCCCATTCGGAATCCAGCGCGTTGACACGCACAATGGTTTCAATATCGCGATACAGCGGATGTTGCAGTGCGTGGTAAACCATGCGGCGGGCGGTGTCTTTTTCACGCAATGCTACGGa                                                                                  <  1:92416/140‑1 (MQ=255)                              cgGCTTCCAGGTCGTTAACACCCCATTCGGAATCCAGCGCGTTGACACGCACAATGGTTTCAATATCGCGATACAGCGGATGTTGCAGTGCGTGGTAAACCATGCGGCGGGCGGTGTCTTTTTCACGCAATGCTACg                                                                                    >  1:73597/1‑137 (MQ=255)                              cgGCTTCCAGGTCGTTAACACCCCATTCGGAATCCAGCGCGTTGACACGCACAATGGTTTCAATATCGCGATACAGCGGATGTTGCAGTGCGTGGTAAACCATGCGGCGGGCGGTGTCTTTTTCACGCAATGCTACg                                                                                    <  2:73597/137‑1 (MQ=255)                                                   cccATTCGGAATCCAGCGCGTTGACACGCACAATGGTTTCAATATCGCGATACAGCGGATGTTGCAGTGCGTGGTAAACCATGCGGCGGGCGGTGTCTTTTTCACGCAATGCTACGGAGTCTTCGAGGTCAAACATCAgg                                                            >  2:119090/1‑140 (MQ=255)                                                                 aGCGCGTTGACACGCACAATGGTTTCAATATCGCGATACAGCGGATGTTGCAGTGCGTGGTAAACCATGCGGCGGGCGGTGTCTTTTTCACGCAATGCTACGGAGTCTTCGAGGTCAAACATCAGGGCAt                                                        >  1:244964/1‑130 (MQ=255)                                                                 aGCGCGTTGACACGCACAATGGTTTCAATATCGCGATACAGCGGATGTTGCAGTGCGTGGTAAACCATGCGGCGGGCGGTGTCTTTTTCACGCAATGCTACGGAGTCTTCGAGGTCAAACATCAGGGCAt                                                        <  2:244964/130‑1 (MQ=255)                                                                                                cgcgATACAGCGGATGTTGCAGTGCGTGGTAAACCATGCGGCGGGCGGGGTCTTTTTCACGCAATGCTACGGAGTCTTCGAGGTCAAACATCAGGGCATCAGCCGGGTAGATGAAGGAGTTGCTGACCATCGCGGCATTg               >  2:340715/1‑140 (MQ=255)                                                                                                  cgATACAGCGGATGTTGCAGTGCGTGGTAAACCATGCGGCGGGCGGTGGCTTTTTCACGCAATGCGATGGGGTCTTTGAGGTCAAAAATCAGGGCATCAGCCGGGAAGATGAAGGGGGTGCTGACCCTCGCGGCATTGGc             >  2:223205/1‑140 (MQ=255)                                                                                                        aGCGGATGTTGCAGTGCGTGGTAAACCATGCGGCGGGCGGTGTCTTTTTCACGCAATGCTACGGAGTCTTCGAGGTCAAACATCAGGGCATCAGCCGGGTAGATGAAGGAGTTGCTGACCATCGCGGCATTGGCACCAgg       <  2:48699/140‑1 (MQ=255)                                                                                                            gATGTTGCAGTGCGTGGTAAACCATGCGGCGGGCGGTGTCTTTTTCACGCAATGCTACGGAGTCTTCGAGGTCAAACATCAGGGCATCAGCCGGGTAGATGAAGGAGTTGCTGACCATCGCGGCATTGGCACCAGGc      <  1:281669/137‑1 (MQ=255)                                                                                                            gATGTTGCAGTGCGTGGTAAACCATGCGGCGGGCGGTGTCTTTTTCACGCAATGCTACGGAGTCTTCGAGGTCAAACATCAGGGCATCAGCCGGGTAGATGAAGGAGTTGCTGACCATCGCGGCATTGGCACCAGGc      >  2:281669/1‑137 (MQ=255)                                                                                                             aTGTTGCAGTGCGTGGTAAACCATGCGGCGGGCGGTGTCTTTTTCACGCAATGCTACGGAGTCTTCGAGGTCAAACATCAGGGCATCAGCCGGGTAGATGAAGGAGTTGCTGACCATCGCGGCATTGGCACCAGGCacaa  <  1:80023/140‑1 (MQ=255)                                                                                                                      |                                                                                                                                   ACGCACAACGTCCGCACCACCGCGAACGACGGCTTCCAGGTCGTTAACACCCCATTCGGAATCCAGCGCGTTGACACGCACAATGGTTTCAATATCGCGATACAGCGGATGTTGCAGCGCGTGGTAAACCATGCGGCGGGCGGTGTCTTTTTCACGCAATGCTACGGAGTCTTCGAGGTCAAACATCAGGGCATCAGCCGGGTAGATGAAGGAGTTGCTGACCATCGCGGCATTGGCACCAGGCACAA  >  NC_000913/650188‑650435

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑