Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	656,905	A→G	T117A (ACC→GCC)	pagP →	Lipid IVA palmitoyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	656,905	0	A	G	100.0%	79.4 / NA	23	T117A (ACC→GCC)	pagP	Lipid IVA palmitoyltransferase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (9/14);  total (9/14)

GACCGTGGGGTGGCGGTTTTGGCCTGTCGCGTTGGGATGAAAAAGGAAACTGGCATGGCCTGTATGCCATGGCATTTAAGGACTCGTGGAACAAATGGGAACCGATTGCCGGATACGGATGGGAAAGTACCTGGCGACCGCTGGCGGATGAAAATTTTCATTTAGGTCTGGGATTCACCGCTGGCGTAACGGCACGCGATAACTGGAATTACATCCCTCTCCCGGTTCTACTGCCATTGGCCTCCGTGGGTTATGGCCCAGT  >  NC_000913/656777‑657038                                                                                                                                 |                                                                                                                                      gACCGTGGGGTGGCGGTTTTGGCCTGTCGCGTTGGGATGAAAAAGGAAACTGGCATGGCCTGTATGCCATGGCATTTAAGGACTCGTGGAACAAATGGGAACCGATTGCCGGATACGGATGGGAAAGTGCCTGGCGACCg                                                                                                                            >  1:60211/1‑140 (MQ=255) gACCGTGGGGTGGCGGTTTTGGCCTGTCGCGTTGGGATGAAAAAGGAAACTGGCATGGCCTGTATGCCATGGCATTTAAGGACTCGTGGAACAAATGGGAACCGATTGCCGGATACGGATGGGAAAGTGCCTGGCGACCg                                                                                                                            >  1:37062/1‑140 (MQ=255)   ccGTGGGGTGGCGGTTTTGGCCTGTCGCGTTGGGATGAAAAAGGAAACTGGCATGGCCTGTATGCCATGGCATTTAAGGACTCGTGGAACAAATGGGAACCGATTGCCGGATACGGATGGGAAAGTGCCTGGCGACCGCt                                                                                                                          <  1:279839/140‑1 (MQ=255)                       ccTGTCGCGTTGGGATGAAAAAGGAAACTGGCATGGCCTGTATGCCATGGCATTTAAGGACTCGTGGAACAAATGGGAACCGATTGCCGGATACGGATGGGAAAGTGCCTGGCGACCGCTGGCGGATGAAAATTTTCAtt                                                                                                      <  2:60211/140‑1 (MQ=255)                           tCGCGTTGGGATGAAAAAGGAAACTGGCATGGCCTGTATGCCATGGCATTTAAGGACTCGTGGAACAAATGGGAACCGATTGCCGGATACGGATGGGAAAGTGCCTGGCGACCGCTGGCGGATGAAAATTTTCATTTAgg                                                                                                  >  1:297808/1‑140 (MQ=255)                                     aTGAAAAAGGAAACTGGCATGGCCTGTATGCCATGGCATTTAAGGACTCGTGGAACAAATGGGAACCGATTGCCGGATACGGATGGGAAAGTGCCTGGCGACCGCTGGCGGATGAAAATTTTCATTTAGGTCTGGGATTc                                                                                        >  1:333488/1‑140 (MQ=255)                                          aaaGGAAACTGGCATGGCCTGTATGCCATGGCATTTAAGGACTCGTGGAACAAATGGGAACCGATTGCCGGATACGGATGGGAAAGTGCCTGGCGACCGCTGGCGGATGAAAATTTTCCTTTAGGTCTGGGATTCACCGc                                                                                   <  2:251972/140‑1 (MQ=255)                                          aaaGGAAACTGGCATGGCCTGTATGCCATGGCATTTAAGGACTCGTGGAACAAATGGGAACCGATTGCCGGATACGGATGGGAAAGTGCCTGGCGACCGCTGGCGGATGAAAATTTTCATTTAGGTCTGGGATTCACCGc                                                                                   <  1:194302/140‑1 (MQ=255)                                           aaGGAAACTGGCATGGCCTGTATGCCATGGCATTTAAGGACTCGTGGAACAAATGGGAACCGATTGCCGGATACGGATGGGAAAGTGCCTGGCGACCGCTGGCGGATGAAAATTTTCATTTAGGTCTGGGATTCACCGCt                                                                                  <  1:21614/140‑1 (MQ=255)                                                           ccTGTATGCCATGGCATTTAAGGACTCGTGGAACAAATGGGAACCGATTGCCGGATACGGATGGGAAAGTGCCTGGCGACCGCTGGCGGATGAAAATTTTCATTTAGGTCTGGGATTCACCGCTGGCGTAACGGCAcgcg                                                                  <  2:37062/140‑1 (MQ=255)                                                                aTGCCATGGCATTTAAGGACTCGTGGAACAAATGGGAACCGATTGCCGGATACGGATGGGAAAGTGCCTGGCGACCGCTGGCGGATGAAAATTTTCATTTAGGTCTGGGATTCACCGCTGGCGTAACGGCACGCGATAAc                                                             <  1:233930/140‑1 (MQ=255)                                                                    cATGGCATTTAAGGACTCGTGGAACAAATGGGAACCGATTGCCGGATACGGATGGGAAAGTGCCTGGCGACCGCTGGCGGATGAAAATTTTCATTTAGGTCTGGGATTCACCGCTGGCGTAACGGCACGCGATAACTGGa                                                         >  1:243791/1‑140 (MQ=255)                                                                    cATGGCATTTAAGGACTCGTGGAACAAATGGGAACCGATTGCCGGATACGGATGGGAAAGTGCCTGGCGACCGCTGGCGGATGAAAATTTTCATTTAGGTCTGGGATTCACCGCTGGCGTAACGGCACGCGATAACTGGa                                                         >  2:254860/1‑140 (MQ=255)                                                                         cATTTAAGGACTCGTGGAACAAATGGGAACCGATTGCCGGATACGGATGGGAAAGTGCCTGGCGACCGCTGGCGGATGAAAATTTTCATTTAGGTCTGGGATTCACCGCTGGCGTAACGGCACGCGATAACTGGAATTAc                                                    >  1:146873/1‑140 (MQ=255)                                                                         cATTTAAGGACTCGTGGAACAAATGGGAACCGATTGCCGGATACGGATGGGAAAGTGCCTGGCGACCGCTGGCGGATGAAAATTTTCATTTAGGTCTGGGATTCACCGCTGGCGTAACGGCACGCGATAACTGGAATTAc                                                    >  2:219060/1‑140 (MQ=255)                                                                                   cTCGTGGAACAAATGGGAACCGATTGCCGGATACGGATGGGAAAGTGCCTGGCGACCGCTGGCGGATGAAAATTTTCATTTAGGTCTGGGATTCACCGCTGGCGTAACGGCACGCGATAACTGGAATTACATCCCTCTcc                                          <  1:254860/140‑1 (MQ=255)                                                                                   cTCGTGGAACAAATGGGAACCGATTGCCGGATACGGATGGGAAAGTGCCTGGCGACCGCTGGCGGATGAAAATTTTCATTTAGGTCTGGGATTCACCGCTGGCGTAACGGCACGCGATAACTGGAATTACATCCCTCTcc                                          <  2:333488/140‑1 (MQ=255)                                                                                      gTGGAACAAATGGGAACCGATTGCCGGATACGGATGGGAAAGTGCCTGGCGACCGCTGGCGGATGAAAATTTTCATTTAGGTCTGGGATTCACCGCTGGCGTAACGGCACGCGATAACTGGAATTACATCCCTCTCCCgg                                       >  1:265745/1‑140 (MQ=255)                                                                                            cAAATGGGAACCGATTGCCGGATACGGATGGGAAAGTGCCTGGCGACCGCTGGCGGATGAAAATTTTCATTTAGGTCTGGGATTCACCGCTGGCGTAACGGCACGCGATAACTGGAATTACATCCCTCTCCCGGTTCTAc                                 <  2:146873/140‑1 (MQ=255)                                                                                                  ggAACCGATTGCCGGATACGGATGGGAAAGTGCCTGGCGACCGCTGGCGGATGAAAATTTTCATTTAGGTCTGGGATTCACCGCTGGCGTAACGGCACGCGATAACTGGAATTACATCCCTCTCCCGGTTCTACTGCCAt                           <  2:265745/140‑1 (MQ=255)                                                                                                           tGCCGGATACGGATGGGAAAGTGCCTGGCGACCGCTGGCGGATGAAAATTTTCATTTAGGTCTGGGATTCACCGCTGGCGTAACGGCACGCGATAACTGGAATTACATCCCTCTCCCGGTTCTACTGCCATTGGCCTCCg                  <  2:243791/140‑1 (MQ=255)                                                                                                                           gAAAGTGCCTGGCGACCGCTGGCGGATGAAAATTTTCATTTAGGTCTGGGATTCACCGCTGGCGTAACGGCACGCGATAACTGGAATTACATCCCTCTCCCGGTTCTACTGCCATTGGCCTCCGTGGGTTATGGCCCAGt  <  2:209225/140‑1 (MQ=255)                                                                                                                           gAAAGTGCCTGGCGACCGCTGGCGGATGAAAATTTTCATTTAGGTCTGGGATTCACCGCTGGCGTAACGGCACGCGATAACTGGAATTACATCCCTCTCCCGGTTCTACTGCCATTGGCCTCCGTGGGTTATGGCCCAGt  <  2:140778/140‑1 (MQ=255)                                                                                                                                 |                                                                                                                                      GACCGTGGGGTGGCGGTTTTGGCCTGTCGCGTTGGGATGAAAAAGGAAACTGGCATGGCCTGTATGCCATGGCATTTAAGGACTCGTGGAACAAATGGGAACCGATTGCCGGATACGGATGGGAAAGTACCTGGCGACCGCTGGCGGATGAAAATTTTCATTTAGGTCTGGGATTCACCGCTGGCGTAACGGCACGCGATAACTGGAATTACATCCCTCTCCCGGTTCTACTGCCATTGGCCTCCGTGGGTTATGGCCCAGT  >  NC_000913/656777‑657038

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑