Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	736,692	T→A	I83N (ATC→AAC)	ybfQ →	inactive transposase YbfQ

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	736,692	0	T	A	100.0%	112.7 / NA	33	I83N (ATC→AAC)	ybfQ	inactive transposase YbfQ
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base A (14/19);  total (14/19)

TTTCTGGTGCAGAAGGTTGGGAAGATATAGAGGATTTCGGGGAAACACATCTCGATTTTTTGAAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACGATACCATTGCCAGAGTTGTATCCCAGGGAAAGATCACGTAACGCTACTTTTTTGTACTAAATAATTCGCATTTTATGTTTAAAAATTGAGATATTCCTTATTACCTAAAGCTGTTTTTCATAGCTTATACATGATCAAATACTCCTTACATAAATAAGGTGAACA  >  NC_000913/736557‑736823                                                                                                                                        |                                                                                                                                    tttCTGGTGCAGAAGGTTGGGAAGATATAGAGGATTTCGGGGAAACACATCTCGATTTTTTGAAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACGATACCATTGCCAGAGTTGTATCCCAGGGAAAGAACACg                                                                                                                                 <  1:245862/140‑1 (MQ=255)           aGAAGGTTGGGAAGATATAGAGGATTTCGGGGAAACACATCTCGATTTTTTGAAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACGATACCATTGCCAGAGTTGTATCCCAGGGAAAGAACACGTAACGCTACt                                                                                                                       >  1:265725/1‑140 (MQ=255)                  tGGGAAGATATAGAGGATTTCGGGGAAACACATCTCGATTTTTTGAAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACGATACCATTGCCAGAGTTGTATCCCAGGGAAAGAACACGTAACGCTACtttt                                                                                                                    <  1:136985/136‑1 (MQ=255)                  tGGGAAGATATAGAGGATTTCGGGGAAACACATCTCGATTTTTTGAAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACGATACCATTGCCAGAGTTGTATCCCAGGGAAAGAACACGTAACGCTACtttt                                                                                                                    >  2:136985/1‑136 (MQ=255)                   gggAAGATATAGAGGATTTCGGGGAAACACATCTCGATTTTTTGAAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACGATACCATTGCCAGAGTTGTATCCCAGGGAAAGAACACGTAACGCTACTTTTTTGTa                                                                                                               <  2:132516/140‑1 (MQ=255)                         atatAGAGGATTTCGGGGAAACACATCTCGATTTTTTGAAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACGATACCATTGCCAGAGTTGTATCCCAGGGAAAGAACACGTAACGCTACTTTTTTGTACTAaat                                                                                                         >  2:143202/1‑140 (MQ=255)                         atatAGAGGATTTCGGGGAAACACATCTCGATTTTTTGAAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACGATACCATTGCCAGAGTTGTATCCCAGGGAAAGAACACGTAACGCTACTTTTTTGTACTAaat                                                                                                         >  2:107739/1‑140 (MQ=255)                              gagGATTTCGGGGAAACACATCTCGATTTTTTGAAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACGATACCATTGCCAGAGTTGTATCCCAGGGAAAGAACACGTAACGCTACTTTTTTGTACTAAATAATTc                                                                                                    >  2:65060/1‑140 (MQ=255)                                      cGGGGAAACACATCTCGATTTTTTGAAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACGATACCATTGCCAGAGTTGTATCCCAGGGAAAGAACACGTAACGCTACTTTTTTGTACTAAATAATTCGCATTTTa                                                                                            <  1:124986/140‑1 (MQ=255)                                           aaaCACATCTCGATTTTTTGAAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACGATACCATTGCCAGAGTTGTATCCCAGGGAAAGAACACGTAACGCTACTTTTTTGTACTAAATAATTCGCATTTTATGttt                                                                                       <  2:129563/140‑1 (MQ=255)                                                 aTCTCGATTTTTTGAAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACGATACCATTGCCAGAGTTGTATCCCAGGGAAAGAACACGTAACGCTACTTTTTTGTACTAAATAATTCGCATTTTATGTTTAAAAAt                                                                                 <  1:65060/140‑1 (MQ=255)                                                         tttttGAAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACGATACCATTGCCAGAGTTGTATCCCAGGGAAAGAACACGTAACGCTACTTTTTTGTACTAAATAATTCGCATTTTATGTTTAAAAATTGAGatat                                                                         <  2:52153/140‑1 (MQ=255)                                                          ttttGAAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACGATACCATTGCCAGAGTTGTATCCCAGGGAAAGAACACGTAACGCTACTTTTTTGTACTAAATAATTCGCATTTTATGTTTAAAAATTGAGATAtt                                                                        <  2:265725/140‑1 (MQ=255)                                                           tttGAAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACGATACCATTGCCAGAGTTGTATCCCAGGGAAAGAACACGTAACGCTACTTTTTTGTACTAAATAATTCGCATTTTATGTTTAAAAATTGAGATATTc                                                                       >  2:252667/1‑140 (MQ=255)                                                                          tGATTTTGAAAATGGTATTCCTGTTCACGATACCATTGCCAGAGTTGTATCCCAGGGAAAGAACACGTAACGCTACTTTTTTGTACTAAATAATTCGCATTTTATGTTTAAAAATTGAGATATTCCTTATTACCTAAAGc                                                        >  1:111755/1‑140 (MQ=255)                                                                          tGATTTTGAAAATGGTATTCCTGTTCACGATACCATTGCCAGAGTTGTATCCCAGGGAAAGAACACGTAACGCTACTTTTTTGTACTAAATAATTCGCATTTTATGTTTAAAAATTGAGATATTCCTTATTACCTAAAGc                                                        >  2:176114/1‑140 (MQ=255)                                                                           gATTTTGAAAATGGTATTCCTGTTCACGATACCATTGCCAGAGTTGTATCCCAGGGAAAGAACACGTAACGCTACTTTTTTGTACTAAATAATTCGCATTTTATGTTTAAAAATTGAGATATTCCTTATTACCTAAAGCt                                                       <  2:176757/140‑1 (MQ=255)                                                                            aTTTTGAAAATGGTATTCCTGTTCACGATACCATTGCCAGAGTTGTATCCCAGGGAAAGAACACGTAACGCTACTTTTTTGTACTAAATAATTCGCATTTTATGTTTAAAAATTGAGATATTCCTTATTACCTAAAGCTg                                                      <  2:354100/140‑1 (MQ=255)                                                                                  aaaaTGGTATTCCTGTTCACGATACCATTGCCAGAGTTGTATTCCAGGGAAAGAACACGTAACGCTACTTTTTTGTACTAAATAATTCGCATTTTATGTTTAAAAATTGAGATATTCCTTATTACCTAAAGCTGTTTTTc                                                >  2:113574/1‑140 (MQ=255)                                                                                  aaaaTGGTATTCCTGTTCACGATACCATTGCCAGAGTTGTATCCCAGGGAAAGAACACGTAACGCTACTTTTTTGTACTAAATAATTCGCATTTTATGTTTAAAAATTGAGATATTCCTTATTACCTAAAGCTGTTTTTc                                                <  2:297667/140‑1 (MQ=255)                                                                                   aaaTGGTATTCCTGTTCACGATACCATTGCCAGAGTTGTATCCCAGGGAAAGAACACGTAACGCTACTTTTTTGTACTAAATAATTCGCATTTTATGTTTAAAAATTGAGATATTCCTTATTACCTAAAGCTGTTTTTCa                                               <  1:180035/140‑1 (MQ=255)                                                                                   aaaTGGTATTCCTGTTCACGATACCATTGCCAGAGTTGTATCCCAGGGAAAGAACACGTAACGCTACTTTTTTGTACTAAATAATTCGCATTTTATGTTTAAAAATTGAGATATTCCTTATTACCTAAAGCTGTTTTTCa                                               <  1:74781/140‑1 (MQ=255)                                                                                       ggTATTCCTGTTCACGATACCATTGCCAGAGTTGTATCCCAGGGAAAGAACACGTAACGCTACTTTTTTGTACTAAATAATTCGCATTTTATGTTTAAAAATTGAGATATTCCTTATTACCTAAAGCTGTTTTTCATAGc                                           <  1:176114/140‑1 (MQ=255)                                                                                          aTTCCTGTTCACGATACCATTGCCAGAGTTGTATCCCAGGGAAAGAACACGTAACGCTACTTTTTTGTACTAAATAATTCGCATTTTATGTTTAAAAATTGAGATATTCCTTATTACCTAAAGCTGTTTTTCATAGCTta                                        <  2:33999/140‑1 (MQ=255)                                                                                                     cGATACCATTGCCAGAGTTGTATCCCAGGGAAAGAACACGTAACGCTACTTTTTTGTACTAAATAATTCGCATTTTATGTTTAAAAATTGAGATATTCCTTATTACCTAAAGCTGTTTTTCATAGCTTATACATGATCaa                             >  1:252130/1‑140 (MQ=255)                                                                                                      gATACCATTGCCAGAGTTGTATCCCAGGGAAAGAACACGTAACGCTACTTTTTTGTACTAAATAATTCGCATTTTATGTTTAAAAATTGAGATATTCCTTATTACCTAAAGCTGTTTTTCATAGCTTATACATGATCaaa                            <  2:25497/140‑1 (MQ=255)                                                                                                             ttGCCAGAGTTGTATCCCAGGGAAAGAACACGTAACGCTACTTTTTTGTACTAAATAATTCGCATTTTATGTTTAAAAATTGAGATATTCCTTATTACCTAAAGCTGTTTTTCATAGCTTATACATGATCAAATACTCCt                     <  2:230004/140‑1 (MQ=255)                                                                                                              tGCCAGAGTTGTATCCCAGGGAAAGAACACGTAACGCTACTTTTTTGTACTAAATAATTCGCATTTTATGTTTAAAAATTGAGATATTCCTTATTACCTAAAGCTGTTTTTCATAGCTTATACATGATCAAATACTCCtt                    >  1:199520/1‑140 (MQ=255)                                                                                                                ccAGAGTTGTATCCCAGGGAAAGAACACGTAACGCTACTTTTTTGTACTAAATAATTCGCATTTTATGTTTAAAAATTGAGATATTCCTTATTACCTAAAGCTGTTTTTCATAGCTTATACATGATCAAATACTCCTTa                   <  2:211764/139‑1 (MQ=255)                                                                                                                ccAGAGTTGTATCCCAGGGAAAGAACACGTAACGCTACTTTTTTGTACTAAATAATTCGCATTTTATGTTTAAAAATTGAGATATTCCTTATTACCTAAAGCTGTTTTTCATAGCTTATACATGATCAAATACTCCTTa                   >  1:211764/1‑139 (MQ=255)                                                                                                                       tGTATCCCAGGGAAAGAACACGTAACGCTACTTTTTTGTACTAAATAATTCGCATTTTATGTTTAAAAATTGAGATATTCCTTATTACCTAAAGCTGTTTTTCATAGCTTATACATGATCAAATACTCCTTACataaata           <  2:287408/140‑1 (MQ=255)                                                                                                                                gggAAAGAACACGTAACGCTACTTTTTTGTACTAAATAATTCGCATTTTATGTTTAAAAATTGAGATATTCCTTATTACCTAAAGCTGTTTTTCATAGCTTATACATGATCAAATACTCCTTACATAAATAAGGTGAACa  >  1:146072/1‑140 (MQ=255)                                                                                                                                gggAAAGAACACGTAACGCTACTTTTTTGTACTAAATAATTCGCATTTTATGTTTAAAAATTGAGATATTCCTTATTACCTAAAGCTGTTTTTCATAGCTTATACATGATCAAATACTCCTTACATAAATAAGGTGAACa  >  1:295519/1‑140 (MQ=255)                                                                                                                                        |                                                                                                                                    TTTCTGGTGCAGAAGGTTGGGAAGATATAGAGGATTTCGGGGAAACACATCTCGATTTTTTGAAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACGATACCATTGCCAGAGTTGTATCCCAGGGAAAGATCACGTAACGCTACTTTTTTGTACTAAATAATTCGCATTTTATGTTTAAAAATTGAGATATTCCTTATTACCTAAAGCTGTTTTTCATAGCTTATACATGATCAAATACTCCTTACATAAATAAGGTGAACA  >  NC_000913/736557‑736823

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑